CN104187584A - Edible fungus multi-mushroom powder product and preparing method thereof - Google Patents
Edible fungus multi-mushroom powder product and preparing method thereof Download PDFInfo
- Publication number
- CN104187584A CN104187584A CN201410344665.4A CN201410344665A CN104187584A CN 104187584 A CN104187584 A CN 104187584A CN 201410344665 A CN201410344665 A CN 201410344665A CN 104187584 A CN104187584 A CN 104187584A
- Authority
- CN
- China
- Prior art keywords
- enzymolysis
- powder
- mushroom
- temperature
- pleurotus nebrodensis
- Prior art date
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- Granted
Links
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/02—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
Abstract
The invention discloses an edible fungus multi-mushroom powder product and belongs to the field of edible fungus deep processing. The edible fungus multi-mushroom powder comprises following components by weight: 5-15 parts of morel enzymolysis powder, 10-20 parts of pleurotus nebrodensis-lentinus edodes enzymolysis powder and 2-5 parts of animal protein hydrolysis powder. The product is prepared by weighing the raw materials according to the formula, mixing uniformly, performing microwave sterilization and packaging. The product has characteristics of outstanding mushroom fragrance, proper taste and rich nutrition. The product can be used as a seasoning and can be eaten together with rice or bread separately. Through enzymolysis treatment, proteins in sporocarp tissues are degraded to a certain degree, the total content of amino acids is increased by 20-37% than the content of amino acids before the enzymolysis treatment. A special high-temperature centrifugation process is adopted, so that the edible-fungus polysaccharide content of the product is increased by 16-35%. By good compatibility of various nutrition components generated by the enzymolysis, the product has a good seasoning function and good nutrition and nourishing functions.
Description
Technical field
The invention belongs to edible mushroom deep processing field, particularly a kind of edible fungus and preparation method thereof.
Background technology
Pleurotus nebrodensis is a kind of rare edible mushroom, according to St Foods Q Supervision & Testing Center, analyzes, and Pleurotus nebrodensis protein content, up to 14.7%, contains 18 seed amino acids in protein, and wherein 8 seed amino acids of needed by human all contain.Every gram of Pleurotus nebrodensis is containing 190 milligrams of polysaccharide.In amino acid, lysine is 569 milligrams/100 grams, is 2.8 times of general flat mushroom; 790.2 milligrams/100 grams of leucines are 2.6 times of general flat mushroom; Isoleucine is 470.1 milligrams/100 grams, is 2.5 times of general flat mushroom; 674.6 milligrams/100 grams of valines, for 2.2 times of general flat mushrooms, other human body essential amino acid content also than the high 1.9-2.7 of general flat mushroom doubly, especially glutamic acid and arginine content are high especially, these are all the nutritional factors that is conducive to intelligence development, more taller than the asparagus content that is called in the world intelligence mushroom.Pleurotus nebrodensis also contains a large amount of multivitamins and multi mineral element.The contained a large amount of mushroom polysaccharide of Pleurotus nebrodensis, can strengthen the immunologic function of human body.Cholesterol in its unrighted acid and blood of human body is combined into cholesterol ester, reduces blood pressure, prevents arteriosclerotic effect.The often edible Pleurotus nebrodensis effect of disease dissipating blood stasis and clearing away heat removing toxic substances that disappears in addition.The nutritive value that Pleurotus nebrodensis is surprising and health care have caused the very big concern of scientific circles.
Hickory chick is under the jurisdiction of Ascomycotina (Ascomycotina), discomycete, Pezizale, Morchellaceae, morchella, and hickory chick has many small ruts with its cap surface, and the outward appearance utmost point is gained the name like sheep tripe.It is a kind of wild famous and precious edible and medical fungi, includes the earliest the > in Li Shizhen (1518-1593 A.D.) < < Compendium of Materia Medica >.The traditional Chinese medical science is used as medicine with Morchella esculenta (L.) Pers sporophore.Hickory chick is foremost delicious edible mushroom in sac fungus, and hickory chick contains protein 22.1%, fat 3.8%, polysaccharide 16.3%; And the unrighted acid in fat is 5 to 3 with the ratio of saturated fatty acid, and the useful linoleic acid of human body is accounted for to 56% of fatty acid total amount.The amino acid of needed by human is complete, and its content is 5 times of Hericium erinaceus.The element that also contains the needed by human such as abundant potassium, phosphorus, calcium, iron, magnesium, zinc and selenium.Wherein the content of potassium, phosphorus is respectively 4 times and 2 times of Cordyceps sinensis, and zinc content is 9 times of Cordyceps sinensis.Selenium can change carcinogenic metabolism direction and anti-oxidant, thereby hickory chick is described as " anticancer star ".
Hickory chick property is flat, and taste is sweet cold, nontoxic, has the function that beneficial stomach, digestion help food, phlegm-eliminiating and qi-regulating, kidney tonifying, establishing-Yang, benefit brain, refresh oneself.Research finds that hickory chick has reducing blood lipid, regulates immunity, antifatigue, anti-radiation, antitumor action, and can alleviate the toxic and side effect that cancer patient's chemicotherapy causes.
Mushroom, claims again mushroom, dried mushroom, is a kind of edible mushroom that human body is had to stronger nutrition health-care functions.According to one's analysis, in 100g fresh mushroom, contain protein 18.4g, fatty 4.8g, carbohydrate 71g (wherein sugar accounts for 35.1g), and contain calcium 120mg, phosphorus 420mg, iron 25mg, vitaminB10 .07mg, vitamin B2 1.13mg, and vitamin C, 30 plurality of enzymes and 16 seed amino acids, also contain in addition rare ergosterol in general vegetables (generating the precursor substance of vitamin D in human body) and there is the compositions such as double stranded RNA, lentinan of immunologic function.
In mushroom leachate, containing the mushroom essence (Lenthionine) of the more 5'-GMP that can produce delicate flavour (5 '-GMP) and generation fragrance, several amino acids and these product taste compositions are used mutually, have just produced the distinctive delicate flavour of mushroom and fragrance.Mushroom contains water-soluble delicate flavour material, can be used as food seasoning, so mushroom important edible and medical fungus and the flavouring that be people.Utilize mushroom raw material, be equipped with suitable auxiliary material and also can process various delicious condiment, so both enriched the kind of food of fungi, improved again the economic benefit of mushroom.
At present to Mushroom production, the research of mushroom processing comes into one's own day by day, < < food and biotechnology journal > > volume the 2nd phase < < Study on Liquid Fermentation Technology of Morchella esculenta > > March the 26th in the 2007th has done to dissolve in research to hickory chick liquid deep layer fermenting, the best medium proportioning of selecting is: corn flour 4.0g/dL, glucose 1.0g/dL, analysis for soybean powder 2.0g/dL, dusty yeast 0.3g/dL, KH
2pO
40.2g/dL, MgSO
40.1g/dL, CaSO
40.1g/dL, selection optimal culture conditions is: 24 ℃, and initial pH5.8, the shaking flask liquid amount of 250mL is 100mL, inoculum concentration 10mL, shaking flask rotating speed 140r/min, fermentation time is 108h.On market, the kind of mushroom converted products mainly concentrates on fresh goods, dried product and three aspects of canned pack, publication number is that China's invention < < mushroom powder and the production technology > > thereof of CN1136408A discloses the edible mushroom powder that is solid powdery, production technology is: fresh mushroom, rinsing, fragmentation, breaking-wall cell, separation, ultrafiltration, oven dry, its technique is simple but nutrition and the reservation of delicate flavour composition are not fine.Publication number is that a China invention < < compound mushroom flavoring of CN101617808A and preparation method thereof > > discloses the flavoring that a kind of primary raw material is agaricus bisporus powder, straw mushroom powder, sodium glutamate etc., wherein the preparation method of mushroom powder is: by after fresh mushroom rinsing, boiling, pulverizing, defibrination, homogeneous, through concentrated, spraying, be dried and make again, it also exists raw material extraction and application rate not high, and nutrition and delicate flavour retain bad problem.Publication number is that the edible fungus composition > > of a CN101455332A China invention < < disease preventing and treating discloses a kind of effectively disease preventing and treating, the edible fungus composition taking good care of one's health, raw material by following percentage by weight forms: auricularia auriculajudae 8-10%, straw mushroom 9-11%, mushroom 7-9%, mushroom 9-11%, asparagus 11-13%, white fungus 10-12%, glossy ganoderma 12-14%, flat mushroom 7-9%, hedgehog hydnum 8-10%, spice 2-3%, flavouring 2-3% and water 4-6%, total amount 100%.First, by auricularia auriculajudae, straw mushroom, mushroom, mushroom, asparagus, white fungus, glossy ganoderma, flat mushroom and hedgehog hydnum cutting slivering or piece, then add spice, flavouring and water, place in pressure cooker, high temperature 120-150 ℃, boiling 5-10 minute, lets cool, tinning or bottling or pack.China invention < < antitumor health care mushroom congee > > that publication number is CN103494180A provides a kind of manufacture craft of antitumor health care mushroom congee, this mushroom congee comprises Hericium erinaceus, northern hickory chick, asparagus, mushroom, white fungus, flat mushroom, pleurotus eryngii, seafood mushroom, crab flavour mushroom, tomato, the seed of Job's tears, rice etc., each raw material is allocated in proportion, selected ingredient requirement green high quality, without disease and pest; Preparation flow comprises the screening of raw material and pretreatment, boiling, tinning, sterilizing etc., and manufacturing process is not added any anticorrisive agent and food additives; It take the edible mushroom with antitumor efficacy is primary raw material, and raw material is fresh fruit body of edible fungi, uses with antitumor vegetables, cereal collocation, has strengthened value and the nutritive value of various raw materials.This mushroom congee comprises various active material as hickory chick element, Flammulin, lentinacin etc., and mushroom taste is strong, nutritious, and enhancing immunity, pre-preventing tumor are had to obvious prevention and health-care effect; And technological operation is simple, suitable batch production is produced.The China invention < < instant edible mushroom that publication number is CN101238878A and preparation method thereof > > provides a kind of preparation method of instant edible mushroom, the flavoring that is characterised in that use comprises mushroom powder, this mushroom powder comprises matsutake dry powder 9-11 by mass parts, Boletus luridus dry powder 18-22, flower mushroom dry powder 9-11, HEIHUZHANG fungus dry powder 9-11, hickory chick dry powder 9-11, Termitomyces albuminosus dry powder 9-11, Boletus aereus dry powder 90-110, Hericium erinaceus dry powder 9-11, there is preparation method's technological process simple, with low cost, the instant product mouthfeel of making is good, and non-greasy has the feature of the strong natural fresh perfume (or spice) of mushroom.Described mushroom powder obtains granularity 40-120 order by corresponding fresh feed bacterium natural air drying to water content lower than 12% rear grinding.
How to make full use of mushroom nutritious, delicious in taste, be rich in the feature of various nutritional labelings, improve raw material extraction, utilization rate, exploitation has the flavoring products of good nutrition health care, meet and enrich the growing material requisite of people, developing edible mushroom new application is edible mushroom deep processing field problem demanding prompt solution.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of many mushrooms of edible mushroom powder, and the preparation method of this many mushrooms of edible mushroom powder is provided.
Described many mushrooms of edible mushroom powder, comprises following parts by weight component: hickory chick enzymolysis powder 5-15, Pleurotus nebrodensis mushroom enzymolysis powder 10-20, animal protein hydrolysis powder 2-5.
Preferably, the ingredients weight parts number of described many mushrooms of edible mushroom powder is composed as follows: hickory chick enzymolysis powder 5-15, Pleurotus nebrodensis mushroom enzymolysis powder 10-20, animal protein hydrolysis powder 2-5, salt 0.05-0.2;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 40-60 ℃ after fermentation ends, adds the cellulase degradation of zymotic fluid weight 0.5-2% and processes, temperature 30-50 ℃, pH4-5, time 0.5-1 hour; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3-1%, hydrolysis temperature 45-50 ℃, pH value 6-7, enzymolysis time 1-2 hour; Adjust temperature to 90-110 ℃ of maintenance 5-10 minute, maintain this temperature centrifugation, keep rotating speed more than 4000rpm; The dry hickory chick enzymolysis powder that obtains of centrifuged supernatant.
Described Pleurotus nebrodensis mushroom enzymolysis powder preparation process is as follows: mushroom, Pleurotus nebrodensis sporophore, after Radix Isatidis and Radix Angelicae Sinensis are pulverized, crushed material mixes with the ratio of 1:3-6 with water, adjustment temperature is 40-60 ℃, pH is 5.0-7.0, add behind mushroom and Pleurotus nebrodensis sporophore quality 0.1-0.3% cellulase degradation 1-1.5 hour, adjust temperature and keep 2-5 minute to 80-95 ℃, adjust again temperature to 50 ℃, add subsequently mushroom and Pleurotus nebrodensis sporophore quality 0.1-0.3% flavor protease carries out enzymolysis, hydrolysis temperature 40-50 ℃, pH value 6-7, enzymolysis time 1-2 hour, control temperature and obtain supernatant 90-95 ℃ of centrifugation, the dry Pleurotus nebrodensis mushroom enzymolysis powder that obtains of supernatant spraying.Described mushroom, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are all crushed to particle diameter below 0.5mm.The parts by weight of mushroom, Pleurotus nebrodensis, Radix Isatidis and Radix Angelicae Sinensis are respectively mushroom 5-8, Pleurotus nebrodensis 3-4, Radix Isatidis 0.2-0.5, Radix Angelicae Sinensis 0.3-1;
Described animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing is mixed with the ratio of 1:5.1-6 with water and through milling treatment of colloid, the gap of adjusting colloid mill stator and rotor is 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour, it is 40-60 ℃ that animal flesh after milling treatment of colloid is adjusted to temperature, pH is 5.0-7.0, the papain enzymolysis 60-100 minute that adds animal flesh quality 0.12-0.25%, be cooled to 5-10 ℃ to keep 1.5-2 hour, increase the temperature to 85-95 ℃ and keep 10-25 minute, control temperature to 40-50 ℃, add animal flesh quality 0.12-0.25% flavor protease and carry out enzymolysis, hydrolysis temperature 40-50 ℃, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, control temperature and keep 2-3 minute at 90 ℃, adjust temperature at 50 ℃ of centrifugal acquisition supernatants, centrifugal rotational speed 4000-5000rpm, centrifugation time 1-2 minute, the dry animal protein hydrolysis powder that obtains of centrifuged supernatant spraying.
Described animal flesh is chicken;
The enzyme preparation of adding in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
In toadstool ferment culture medium, add the root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10-20%;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method are as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized to 100 mesh sieves, crushed mixture is added to 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40-60 ℃, pH is 5.0-7.0, keeps 30-60 minute, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
Product preparation process of the present invention is as follows:
By formula, take each raw material, mix rear microwave disinfection, packing is product.
Product of the present invention can directly eat or be dissolved in water edible, also can add in other food and use.
Beneficial effect
It is outstanding that this product has mushroom fragrance, and taste is suitable, and nutritious feature both can be made flavoring, can help separately food again, is the good merchantable brand of house tourism.By enzymolysis processing, make protein in its fructification tissue obtain degraded to a certain extent, utilize amino acid analyzer to measure, total amino acid content has improved 20-37% than non-enzymolysis processing; And owing to adopting special high temperature centrifugal separation process to effectively raise the water-soluble effect of product of the present invention, make edible fungi polysaccharide content in product improve 16-35% simultaneously, effectively improved product quality; Owing to adding animal protein factor digest, make product of the present invention delicious in taste, the while, due to the good compatibility of the various nutritional labelings of enzymolysis generation, makes product of the present invention not only have good seasoning function but also has good nutrition nourishing function.
The preparation of animal protein hydrolysis powder, adopt high-pressure homogeneous, enzymolysis high-temperature process in mid-term subsection enzymolysis treatment process to combine, being beneficial to enzyme plays a role, improve enzymolysis efficiency and nutriment stripping, nutritive value and the good taste of product have been improved, this product is good mouthfeel under the condition without flavouring, and taste appraisal score value is used flavouring product higher than related category.
This product special flavour is delicious, is rich in multiple nutrients functional component, is a kind of edible mushroom deep processed product that collects nutrition, nourishing, adjusting immunity of human body function.This product instant, can be used as common food flavoring or chafing dish bottom flavorings edible, also direct-edible.
The specific embodiment
The following examples can make the present invention of those skilled in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
Many mushrooms of edible mushroom powder, parts by weight are composed as follows: hickory chick enzymolysis powder 10, Pleurotus nebrodensis mushroom enzymolysis powder 15, animal protein hydrolysis powder 3, salt 0.2;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 45 ℃ after fermentation ends, adds the cellulase degradation of zymotic fluid weight 1% and processes, temperature 45 C, pH5,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.5%, 50 ℃ of hydrolysis temperatures, pH value 7, enzymolysis time 2 hours; Adjust temperature to 100 ℃ and keep 6 minutes, maintain this temperature centrifugation, keep rotating speed at 4000rpm; The dry hickory chick enzymolysis powder that obtains of centrifuged supernatant.
Described Pleurotus nebrodensis mushroom enzymolysis powder preparation process is as follows: mushroom, Pleurotus nebrodensis sporophore, after Radix Isatidis and Radix Angelicae Sinensis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjusting temperature is 50 ℃, pH is 6.0, add the cellulase degradation of mushroom and Pleurotus nebrodensis sporophore quality 0.2% after 1 hour, adjusting temperature to 90 ℃ keeps 3 minutes, adjust again temperature to 50 ℃, add subsequently mushroom and Pleurotus nebrodensis sporophore quality 0.1% flavor protease carries out enzymolysis, 40 ℃ of hydrolysis temperatures, pH value 6, enzymolysis time 1 hour, control temperature and obtain supernatant 95 ℃ of centrifugations, the dry Pleurotus nebrodensis mushroom enzymolysis powder that obtains of supernatant spraying.Described mushroom, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are all crushed to particle diameter below 0.5mm.The parts by weight of mushroom, Pleurotus nebrodensis, Radix Isatidis and Radix Angelicae Sinensis are respectively mushroom 6, Pleurotus nebrodensis 3, Radix Isatidis 0.5, Radix Angelicae Sinensis 0.3;
Described animal protein hydrolysis powder, preparation method thereof is as follows: get animal flesh and rub through meat grinder, animal flesh after rubbing is mixed with the ratio of 1:6 with water and through milling treatment of colloid, the gap of adjusting colloid mill stator and rotor is 105 microns, and colloid mill flow-control is 0.3 ton/hour, it is 50 ℃ that animal flesh after milling treatment of colloid is adjusted to temperature, pH is 6.0, the papain enzymolysis 80 minutes that adds animal flesh quality 0.20%, be cooled to 8 ℃ to keep 1.5 hours, increasing the temperature to 90 ℃ keeps 15 minutes, control temperature to 45 ℃, add animal flesh quality 0.2% flavor protease and carry out enzymolysis, 50 ℃ of hydrolysis temperatures, pH value 7, enzymolysis time 1 hour, obtain animal protein factor digest, control temperature and keep 2-3 minute at 90 ℃, adjust temperature at 50 ℃ of centrifugal acquisition supernatants, centrifugal rotational speed 4500rpm, centrifugation time 2 minutes, the dry animal protein hydrolysis powder that obtains of centrifuged supernatant spraying.
Described animal flesh is chicken;
The preparation method of hickory chick is as follows:
(1) preparation of described fermented morel liquid: adopt slant strains to spread cultivation step by step and obtain hickory chick fungal fermented filtrate;
(1) first order seed is cultivated: hickory chick slant strains is accessed in 500 ml shake flasks to 100 milliliters of fermentation medium loading amounts, 180 revs/min of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of fermentation medium loading amounts, 100 revs/min of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 10% inoculum concentration access total measurement (volume) is 150L, fermentation medium loading amount 100L, 24 ℃ of cultivation temperature, 150 revs/min of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed tank is cultivated: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification be take to 10% inoculum concentration access total measurement (volume), 1 ton of fermentation medium loading amount, and condition of culture is identical with first class seed pot;
(6) fermentation tank culture: the bacterial classification in secondary seed tank be take to the fermentation tank that 10% inoculum concentration access total measurement (volume) is 13 tons, 10 tons of fermentation medium loading amounts, 24 ℃ of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:0.4, tank pressure 0.05Mpa, incubation time 72 hours.
Described above-mentioned fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn, 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 15%; Insufficient section water is supplied, pH7.0-7.2,121 ℃ of high pressure steam sterilization 20min.
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method are as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized to 100 orders, crushed mixture is added to 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjusting temperature is 60 ℃, pH is 7.0, keeps 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
The enzyme preparation of adding in the present invention is purchased from letter enzyme preparation company of Novi.
Product preparation process of the present invention is as follows:
By formula, take each raw material, mix rear microwave disinfection, packing is product.
It is outstanding that this product has mushroom fragrance, and taste is suitable, and total amino acid content has improved 20% than non-enzymolysis processing; And due in conjunction with adopting high temperature centrifugal separation process to effectively raise the water-soluble effect of product of the present invention, make edible fungi polysaccharide content in product improve 18% simultaneously, effectively improved product quality; Owing to adding animal protein factor digest, make product of the present invention delicious in taste, the good compatibility of while due to the various nutritional labelings that enzymolysis produces makes product of the present invention not only have good seasoning function but also has good nutrition nourishing function.
Embodiment 2
Many mushrooms of edible mushroom powder, parts by weight are composed as follows: hickory chick enzymolysis powder 15, Pleurotus nebrodensis mushroom enzymolysis powder 10, animal protein hydrolysis powder 2, salt 0.05;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 60 ℃ after fermentation ends, adds the cellulase degradation of zymotic fluid weight 2% and processes, temperature 50 C, pH4.5,0.5 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 1%, 45 ℃ of hydrolysis temperatures, pH value 6.5, enzymolysis time 1 hour; Adjust temperature to 100 ℃ and keep 5 minutes, maintain this temperature centrifugation, keep rotating speed at 4500rpm; The dry hickory chick enzymolysis powder that obtains of centrifuged supernatant.
Pleurotus nebrodensis mushroom enzymolysis powder preparation process is as follows: mushroom, Pleurotus nebrodensis sporophore, after Radix Isatidis and Radix Angelicae Sinensis are pulverized, crushed material mixes with the ratio of 1:6 with water, adjusting temperature is 60 ℃, pH is 7.0, add the cellulase degradation of mushroom and Pleurotus nebrodensis sporophore quality 0.2% after 1.5 hours, adjusting temperature to 80 ℃ keeps 5 minutes, adjust again temperature to 50 ℃, add subsequently mushroom and Pleurotus nebrodensis sporophore quality 0.3% flavor protease carries out enzymolysis, 50 ℃ of hydrolysis temperatures, pH value 7, enzymolysis time 2 hours, control temperature and obtain supernatant 90 ℃ of centrifugations, the dry Pleurotus nebrodensis mushroom enzymolysis powder that obtains of supernatant spraying.Described mushroom, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are all crushed to particle diameter below 0.5mm.The parts by weight of mushroom, Pleurotus nebrodensis, Radix Isatidis and Radix Angelicae Sinensis are respectively mushroom 8, Pleurotus nebrodensis 4, Radix Isatidis 0.2, Radix Angelicae Sinensis 0.5;
Described animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing is mixed with the ratio of 1:5.4 with water and through milling treatment of colloid, the gap of adjusting colloid mill stator and rotor is 105 microns, and colloid mill flow-control is 0.3 ton/hour, it is 60 ℃ that animal flesh after milling treatment of colloid is adjusted to temperature, pH is 7.0, the papain enzymolysis 100 minutes that adds animal flesh quality 0.12%, be cooled to 5 ℃ to keep 2 hours, increasing the temperature to 95 ℃ keeps 25 minutes, control temperature to 50 ℃, add animal flesh quality 0.12% flavor protease and carry out enzymolysis, 45 ℃ of hydrolysis temperatures, pH value 6, enzymolysis time 21 hours, obtain animal protein factor digest, controlling temperature keeps 2 minutes at 90 ℃, adjust temperature at 50 ℃ of centrifugal acquisition supernatants, centrifugal rotational speed 5000rpm, centrifugation time 1 minute, the dry animal protein hydrolysis powder that obtains of centrifuged supernatant spraying.
Described animal flesh is chicken;
The preparation method of hickory chick is as follows:
(1) preparation of described fermented morel liquid: adopt slant strains to spread cultivation step by step and obtain hickory chick fungal fermented filtrate;
(1) first order seed is cultivated: hickory chick slant strains is accessed in 500 ml shake flasks to 100 milliliters of fermentation medium loading amounts, 180 revs/min of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of fermentation medium loading amounts, 100 revs/min of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 10% inoculum concentration access total measurement (volume) is 150L, fermentation medium loading amount 100L, 24 ℃ of cultivation temperature, 150 revs/min of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed tank is cultivated: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification be take to 10% inoculum concentration access total measurement (volume), 1 ton of fermentation medium loading amount, and condition of culture is identical with first class seed pot;
(6) fermentation tank culture: the bacterial classification in secondary seed tank be take to the fermentation tank that 10% inoculum concentration access total measurement (volume) is 13 tons, 10 tons of fermentation medium loading amounts, 24 ℃ of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:0.4, tank pressure 0.05Mpa, incubation time 72 hours.
Described above-mentioned fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn, 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 10%; Insufficient section water is supplied, pH7.0-7.2,121 ℃ of high pressure steam sterilization 20min.
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method are as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized to 100 orders, crushed mixture is added to 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjusting temperature is 60 ℃, pH is 7.0, keeps 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
The enzyme preparation of adding in the present invention is purchased from letter enzyme preparation company of Novi.
Product preparation process of the present invention is as follows:
By formula, take each raw material, mix rear microwave disinfection, packing is product.
It is outstanding that this product has mushroom fragrance, and taste is suitable, and total amino acid content has improved 20% than non-enzymolysis processing; And owing to adopting special high temperature centrifugal separation process to effectively raise the water-soluble effect of product of the present invention, make edible fungi polysaccharide content in product improve 18% simultaneously, effectively improved product quality; Owing to adding animal protein factor digest, make product of the present invention delicious in taste, simultaneously due to enzymolysis Radix Isatidis and, Radix Angelicae Sinensis, the root of bidentate achyranthes and the bark of eucommia effectively discharge the active ingredient of Chinese medicine, improved wherein the result of extraction of plant polyose, in product, plant polyose is processed through enzymolysis and zymotechnique, and in hickory chick enzymolysis powder, the content of plant polyose improves than contrast (there is no the enzymolysis processing root of bidentate achyranthes and the bark of eucommia) and improved 8-19%; Therefore, the good compatibility of the various nutritional labelings of product of the present invention makes product of the present invention not only have good seasoning function but also has good nutrition nourishing function.
Embodiment 3
Basic skills is with example 1 and example 2;
Many mushrooms of edible mushroom powder, parts by weight are composed as follows: hickory chick enzymolysis powder 5, Pleurotus nebrodensis mushroom enzymolysis powder 20, animal protein hydrolysis powder 5, salt 0.1;
Described fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn, 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 20%; Insufficient section water is supplied, pH7.0-7.2,121 ℃ of high pressure steam sterilization 20min.
Product preparation process of the present invention is as follows:
By formula, take each raw material, mix rear microwave disinfection, packing is product.
The embodiment of the present invention 1 product selects Beijing area 100 ages in the elderly's edible January continuously of weak easy catching a cold of 60-75 year in the winter time, edible 100 grams of every day, to eat front and edible rear effect comparison, the results are shown in Table 1, result shows that this product has positive effect to raising the elderly resistance, enhancing body immunity, and product has good edible and nutritious tonifying effect.Simultaneously can significantly improve sleep quality, improvement rate reaches 40%, and (take have quality be the length of one's sleep assessment according to) many continuously edible this product old men reflect that to take for a long time effect better, and have no interest the whole year, and health is better;
The table 1 eating effect table of comparisons
Sensory evaluation experiment
Invite 30 personnel to carry out sense organ marking to the product of the embodiment of the present invention 1 preparation, each 30 of personnel; Marking comprises outward appearance (20 minutes), quality (25 minutes), local flavor (30 minutes), chews four aspects of rear residue sense (25 minutes), and marking personnel independently carry out, and are independent of each other, accurate to guarantee judging result.To judging result, add up, all score value is got approximation, retains integer, specifically in Table 1:
Table 1 sensory evaluation statistics
Note: show significant difference (P < 0.05) with marking different lowercase alphabets in a line, mark different capitalizations and represent difference extremely significantly (P < 0.01), indicate same letter and represent difference not remarkable (P > 0.05).
Above result shows, product prepared by the present invention has good sensory manifestation from outward appearance, quality, local flavor and mouthfeel.
Claims (9)
1. many mushrooms of edible mushroom powder, comprises following parts by weight component: hickory chick enzymolysis powder 5-15, Pleurotus nebrodensis mushroom enzymolysis powder 10-20, animal protein hydrolysis powder 2-5.
2. many mushrooms of edible mushroom powder according to claim 1, is characterized in that, preferred, the ingredients weight parts number of described many mushrooms of edible mushroom powder is composed as follows: hickory chick enzymolysis powder 5-15, Pleurotus nebrodensis mushroom enzymolysis powder 10-20, animal protein hydrolysis powder 2-5, salt 0.05-0.2.
3. according to many mushrooms of edible mushroom powder described in claim 1 or 2, it is characterized in that, the preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, after fermentation ends, adjust to 40-60 ℃, adding the cellulase degradation of zymotic fluid weight 0.5-2% processes, temperature 30-50 ℃, pH4-5, time 0.5-1 hour; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3-1%, hydrolysis temperature 45-50 ℃, pH value 6-7, enzymolysis time 1-2 hour; Adjust temperature to 90-110 ℃ of maintenance 5-10 minute, maintain this temperature centrifugation, keep rotating speed more than 4000rpm; The dry hickory chick enzymolysis powder that obtains of centrifuged supernatant.
4. many mushrooms of edible mushroom powder according to claim 3, it is characterized in that, described Pleurotus nebrodensis mushroom enzymolysis powder preparation process is as follows: mushroom, Pleurotus nebrodensis sporophore, after Radix Isatidis and Radix Angelicae Sinensis are pulverized, crushed material mixes with the ratio of 1:3-6 with water, adjustment temperature is 40-60 ℃, pH is 5.0-7.0, add behind mushroom and Pleurotus nebrodensis sporophore quality 0.1-0.3% cellulase degradation 1-1.5 hour, adjust temperature and keep 2-5 minute to 80-95 ℃, adjust again temperature to 50 ℃, add subsequently mushroom and Pleurotus nebrodensis sporophore quality 0.1-0.3% flavor protease carries out enzymolysis, hydrolysis temperature 40-50 ℃, pH value 6-7, enzymolysis time 1-2 hour, control temperature and obtain supernatant 90-95 ℃ of centrifugation, the dry Pleurotus nebrodensis mushroom enzymolysis powder that obtains of supernatant spraying, described mushroom, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are all crushed to particle diameter below 0.5mm, the parts by weight of mushroom, Pleurotus nebrodensis, Radix Isatidis and Radix Angelicae Sinensis are respectively mushroom 5-8, Pleurotus nebrodensis 3-4, Radix Isatidis 0.2-0.5, Radix Angelicae Sinensis 0.3-1.
5. according to many mushrooms of edible mushroom powder described in claim 1 or 2, it is characterized in that, described animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing is mixed with the ratio of 1:5.1-6 with water and through milling treatment of colloid, the gap of adjusting colloid mill stator and rotor is 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour, it is 40-60 ℃ that animal flesh after milling treatment of colloid is adjusted to temperature, pH is 5.0-7.0, the papain enzymolysis 60-100 minute that adds animal flesh quality 0.12-0.25%, be cooled to 5-10 ℃ to keep 1.5-2 hour, increase the temperature to 85-95 ℃ and keep 10-25 minute, control temperature to 40-50 ℃, add animal flesh quality 0.12-0.25% flavor protease and carry out enzymolysis, hydrolysis temperature 40-50 ℃, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, control temperature and keep 2-3 minute at 90 ℃, adjust temperature at 50 ℃ of centrifugal acquisition supernatants, centrifugal rotational speed 4000-5000rpm, centrifugation time 1-2 minute, the dry animal protein hydrolysis powder that obtains of centrifuged supernatant spraying.
6. many mushrooms of edible mushroom powder according to claim 3, is characterized in that, adds the root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10-20% in described toadstool ferment culture medium; The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method are as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized to 100 orders, crushed mixture is added to 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40-60 ℃, pH is 5.0-7.0, keeps 30-60 minute, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
7. many mushrooms of edible mushroom powder according to claim 2, is characterized in that, parts by weight are composed as follows: hickory chick enzymolysis powder 10, Pleurotus nebrodensis mushroom enzymolysis powder 15, animal protein hydrolysis powder 3, salt 0.2.
8. according to the preparation method of many mushrooms of edible mushroom powder described in the arbitrary claim of claim 1-7, it is characterized in that, by formula, take each raw material, mix rear microwave disinfection, packing is product.
9. the preparation method of many mushrooms of edible mushroom powder according to claim 7, is characterized in that, described many mushrooms of edible mushroom powder parts by weight are composed as follows: hickory chick enzymolysis powder 10, Pleurotus nebrodensis mushroom enzymolysis powder 15, animal protein hydrolysis powder 3, salt 0.2;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 45 ℃ after fermentation ends, adds the cellulase degradation of zymotic fluid weight 1% and processes, temperature 45 C, pH5,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.5%, 50 ℃ of hydrolysis temperatures, pH value 7, enzymolysis time 2 hours; Adjust temperature to 100 ℃ and keep 6 minutes, maintain this temperature centrifugation, keep rotating speed at 4000rpm; The dry hickory chick enzymolysis powder that obtains of centrifuged supernatant;
Described Pleurotus nebrodensis mushroom enzymolysis powder preparation process is as follows: mushroom, Pleurotus nebrodensis sporophore, after Radix Isatidis and Radix Angelicae Sinensis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjusting temperature is 50 ℃, pH is 6.0, add the cellulase degradation of mushroom and Pleurotus nebrodensis sporophore quality 0.2% after 1 hour, adjusting temperature to 90 ℃ keeps 3 minutes, adjust again temperature to 50 ℃, add subsequently mushroom and Pleurotus nebrodensis sporophore quality 0.1% flavor protease carries out enzymolysis, 40 ℃ of hydrolysis temperatures, pH value 6, enzymolysis time 1 hour, control temperature and obtain supernatant 95 ℃ of centrifugations, the dry Pleurotus nebrodensis mushroom enzymolysis powder that obtains of supernatant spraying, described mushroom, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are all crushed to particle diameter below 0.5mm, the parts by weight of mushroom, Pleurotus nebrodensis, Radix Isatidis and Radix Angelicae Sinensis are respectively mushroom 6, Pleurotus nebrodensis 3, Radix Isatidis 0.5, Radix Angelicae Sinensis 0.3,
Described animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing is mixed with the ratio of 1:6 with water and through milling treatment of colloid, the gap of adjusting colloid mill stator and rotor is 105 microns, and colloid mill flow-control is 0.3 ton/hour, it is 50 ℃ that animal flesh after milling treatment of colloid is adjusted to temperature, pH is 6.0, the papain enzymolysis 80 minutes that adds animal flesh quality 0.20%, be cooled to 8 ℃ to keep 1.5 hours, increasing the temperature to 90 ℃ keeps 15 minutes, control temperature to 45 ℃, add animal flesh quality 0.2% flavor protease and carry out enzymolysis, 50 ℃ of hydrolysis temperatures, pH value 7, enzymolysis time 1 hour, obtain animal protein factor digest, control temperature and keep 2-3 minute at 90 ℃, adjust temperature at 50 ℃ of centrifugal acquisition supernatants, centrifugal rotational speed 4500rpm, centrifugation time 2 minutes, the dry animal protein hydrolysis powder that obtains of centrifuged supernatant spraying,
Described animal flesh is chicken;
The preparation method of hickory chick is as follows:
(1) preparation of described fermented morel liquid: adopt slant strains to spread cultivation step by step and obtain hickory chick fungal fermented filtrate;
(1) first order seed is cultivated: hickory chick slant strains is accessed in 500 ml shake flasks to 100 milliliters of fermentation medium loading amounts, 180 revs/min of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of fermentation medium loading amounts, 100 revs/min of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 10% inoculum concentration access total measurement (volume) is 150L, fermentation medium loading amount 100L, 24 ℃ of cultivation temperature, 150 revs/min of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed tank is cultivated: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification be take to 10% inoculum concentration access total measurement (volume), 1 ton of fermentation medium loading amount, and condition of culture is identical with first class seed pot;
(6) fermentation tank culture: the bacterial classification in secondary seed tank be take to the fermentation tank that 10% inoculum concentration access total measurement (volume) is 13 tons, 10 tons of fermentation medium loading amounts, 24 ℃ of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:0.4, tank pressure 0.05Mpa, incubation time 72 hours;
Described above-mentioned fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn, 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 15%; Insufficient section water is supplied, pH7.0-7.2,121 ℃ of high pressure steam sterilization 20min;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method are as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized to 100 orders, crushed mixture is added to 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjusting temperature is 60 ℃, pH is 7.0, keeps 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
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