CN104171643B - Get rid of neat lay heavily laying hen 16 week old-2% laying rate rejection crown material of hat - Google Patents
Get rid of neat lay heavily laying hen 16 week old-2% laying rate rejection crown material of hat Download PDFInfo
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Abstract
This divisional application discloses gets rid of the neat lay heavily laying hen 16 week old 2% laying rate rejection crown material of hat, during laying hen 16 week old 2% laying rate, promotes organ of multiplication to grow and body weight increases, belong to fodder compound field.Described rejection crown material is mainly made up of the raw material of following parts by weight: Semen Maydis 54.365 parts, 3 parts of Testa oryzae, Testa Tritici 2.5 parts, bean cake 14 parts, fermented bean cake 3 parts, Semen arachidis hypogaeae dregs 3 parts, sunflower meal 2.8 parts, rapeseed meal 2 parts, DDGS3.5 part, corn bran 3 parts, 1.0 parts of Oleum Glycines, stone powder 5.2 parts, calcium hydrogen phosphate 0.8 part, Sal 0.35 part, methionine 0.13 part, 70 lysine sulfate 0.12 part, probiotics 0.05 part, compound enzymic preparation 0.05 part, phytase 0.012 part, compound premix 1 part.Can reaching to get rid of the hat phase, to get rid of hat neat, and initial stage egg size of laying eggs is big, and that lays eggs on peak is fast, lay heavily effect.
Description
The application is divisional application, original application application number: 201310061331.1, the applying date: 2013-02-25, denomination of invention:
Uniform-crowning high-egg-yield laying hen nutritional set feed application method and feedstuff thereof.
Technical field
The invention belongs to fodder compound and raising field, (16 week old 2% are laid eggs to be used for promoting laying hen more particularly to one
Rate) organ of multiplication grow and body weight increase fodder compound and feeding method.
Background technology
Commodity egg has body weight to increase the special physiological features such as fast, organ of multiplication growth is fast in the 16-28 week old stage, there is physiology
The anorexia phase, feed intake is inadequate, and the nutrition of the needs such as growth of laying eggs, body weight increase, organ of multiplication maturation rapidly is high, thus produces
Raw huge stress, causes Five problems:
First: on once, sick (nutrition deposit is not) is come on peak；
Change lay eggs peak material at the second: one, and chicken begins to diarrhoea.Medication takes a turn for the better, and drug withdrawal is just violated, and is difficult to cure, result eggshell
Tarnish, lays eggs without peak (transition)；
3rd: slow on peak, peak is lasting, egg size undesirable (adjust, lay in)；
4th: initial stage paralysis lower limb of laying eggs, suppress egg, double-yolked egg too much, lay eggs 3,4 months after the chicken many (marrow bone development) of paralysis lower limb；
5th: baffled death (is grown, laid in), experience stress be big, and death rate is high.
Problem above causes laying hen egg yield low, economic benefit extreme difference.With developed country's ratio, the product of one chicken during Chinese 72 week old
Egg amount is 16-18 kilogram, and developed country is 19-20 kilogram, the low 2 kilograms of eggs of chicken, loses 8 yuans, ten thousand chicken houses
The concealed loss of 1 year is exactly 80,000 yuan.The reason producing gap is not only that feed nutrition concentration is inadequate, also has feeding and management rank
Section divides thin, and some feed factory 16 week old Direct-feds are laid eggs peak material, and scale of feeding is accurate not, and searching for food, it is the most former to wait not
Cause.
Egg Production of Laying Hens peak feedstuff in the market is essentially all 16 week old and begins to use the laying hen material to 72 week old undercarriages,
Divided stages is not thin, owing to calcium height causes just laying hen diarrhoea phenomenon serious；Do not meet laying hen and open antenatal and upper peak special physiological
The demand in stage, causes chicken farm hidden loss huge, shows that death rate is high, and egg production is low, and breeding cycle is short, 65 had
Week old is just eliminated.According to the breeding layer chicken amount of China, this hidden loss and foodstuff waste are immeasurable.
Summary of the invention
The present invention seeks to allow laying hen open antenatal and peak early stage of laying eggs, rational nutrition transition (mainly calcium level), fully
Search for food high nutrition, high digestibility daily ration, rich in improving the additive of reproductive performance, promote that organ of multiplication is grown rapidly, maximum limit
Degree body weight increases, and guarantees that body constitution is strong, and premunition anti-stress ability is strong, promotes body maturation growth Tong Bu with sexual maturity, improves
Egg size, peak of laying eggs be rapidly reached 95% even more than, establish good basis for maintaining peak persistency to improve lifelong egg production.
To achieve these goals, the technical solution used in the present invention is: get rid of the most lay heavily 168 laying hen nutrition set meal application of hat
Pattern.This set meal is divided into two stages and corresponding two kinds of feedstuffs, and one is rejection crown material: 16 week old are to 2% laying rate；Two is to lay eggs
Peak early stage material: 2% laying rate is to 28 week old.
During 16 16 week old, chicken group 20-30% cockscomb starts to feed rejection crown material when reddening, and every 1000 chickens are accumulative uses about 1.6 tons,
Reach 2% to laying rate to start to feed peak early stage material；8 peak early stages use, and 2% laying rate-28 week old feeds height of laying eggs
Peak early stage mixed feed, every 1000 chickens are accumulative uses about 8 tons.
Described rejection crown material is made up of the raw material of following parts by weight:
Semen Maydis 54.365 parts, 3 parts of Testa oryzae, Testa Tritici 2.5 parts, bean cake 14 parts, fermented bean cake 3 parts, Semen arachidis hypogaeae dregs 3 parts, certain herbaceous plants with big flowers
The flower dregs of rice 2.8 parts, rapeseed meal 2 parts, DDGS3.5 part, corn bran 3 parts, 1.0 parts of Oleum Glycines, stone powder 5.2 parts, calcium hydrogen phosphate
0.8 part, Sal 0.35 part, methionine 0.13 part, 70 lysine sulfate 0.12 part, probiotics 0.05 part, compound enzyme system
Agent 0.05 part, phytase 0.012 part, compound premix 1 part, choline chloride 0.1 part, yeast selenium 0.002 part；
Nutritive index is designed as metabolizable energy 2700 kcal/kg, crude protein 17.0%, apparent digestible lysine 0.72%, can digest egg
+ Guang 0.64%, calcium 2.2%, total phosphorus 0.55%.
Described peak early stage material of laying eggs is made up of the raw material of following parts by weight:
Semen Maydis 55.945 parts, 2 parts of Testa oryzae, bean cake 15 parts, fermented bean cake 1.5 parts, Semen arachidis hypogaeae dregs 1 part, sunflower meal 3.1 parts,
Rapeseed meal 2.5 parts, DDGS2.7 part, corn bran 4 parts；1.0 parts of Oleum Glycines, stone powder 8.8 parts, Sal 0.35 part, methionine
0.12 part, 70 lysine sulfate 0.15 part, probiotics 0.05 part, compound enzymic preparation 0.05 part, phytase 0.012 part,
Compound premix 1 part, choline chloride 0.1 part, yeast selenium 0.002 part；
Nutritive index is designed as metabolizable energy 2700 kcal/kg, crude protein 16.5%, apparent digestible lysine 0.70%, can digest egg
+ Guang 0.63%, calcium 3.5%, total phosphorus 0.52%.
In described compound premix, the parts by weight content of each component is as follows:
Zinc gluconate 15 parts, Fe Lys 30 parts；Methionine chelate copper 2 parts；Copper chloride hydroxide 2 parts, aminoacid
Chelated Manganese 40 parts；Chromium picolinate 1.5 parts；Yeast selenium 1.5 parts；Calcium iodate 4 parts；Cobaltous chloride 0.5 part.Vitamin A accounts for
9 parts, vitamin D33 parts, vitamin E 11 parts, vitamin K3Account for 1 part, vitamin B10.4 part, vitamin B22 parts,
Vitamin B61.5 parts, vitamin B120.5 part, nicotiamide 16 parts, calcium pantothenate 4 parts, biotin 0.5 part.
In described compound enzymic preparation, the parts by weight content of each component is as follows: xylanase 2-3 part, mannase 1-4 part,
Protease 3-4 parts, amylase 3-5 part；
The enzyme activity unit of above-mentioned enzyme is as follows: xylanase 20000-30000U/g, mannase 1000-40000U/g, egg
White enzyme 3000-5000U/g, amylase 3000-6000U/g.
The parts by weight content of each component of described probiotics is as follows: bacillus subtilis microbial agent 1-3 part, lactobacillus rhamnosus microbial inoculum
6-10 part, saccharomyces cerevisiae microbial inoculum 1-3 part.
Preparation method: by above-mentioned raw materials by after proportioning feeding, be sufficiently mixed granulation, reach between granularity 1.0-2.5mm 50% with
On.Then granule and probiotics are mixed.
Prepared by bacillus subtilis microbial agent: from inclined-plane, bacillus subtilis is cultivated in switching, and the seed liquor after spreading cultivation step by step is transferred into sending out
In ferment tank, controlling temperature and be 28-30 DEG C, aerlbic culture 19-24 hour, ventilation is 2.0m3/ minute；Ferment complete centrifugal point
From obtaining wet thallus, adding protective agent lyophilization and obtain microbial inoculum, protective agent consists of 10% defatted milk, 5% lactose, 5% sweet
Oil.
Prepared by saccharomyces cerevisiae bacteria agent: slant strains through conventional multistage spread cultivation technique obtain yeast starter liquid, transfer in fermentation tank,
Controlling temperature is 28 DEG C, early stage aerlbic culture 14 hours, and ventilation controls as 2.0m3/ minute, later stage Anaerobic culturel 15 hours；
Fermentation liquid cryoconcentration, after mixing with carrier, prepares through fluid bed drying, and vehicle group becomes CaCO340 parts, 20 parts of dextrin,
Zein powder 20 parts.
Prepared by lactobacillus rhamnosus: slant culture lactobacillus rhamnosus seed liquor is transferred in fermentation tank, controls temperature and is 28-32 DEG C,
Anaerobic culturel 22 hours, ventilation is 2.0m3/ minute；Complete centrifugation of fermenting obtains wet thallus, and addition consists of 10% and takes off
Fat milk, 5% trehalose, 5% glycerol and the protective agent of 5% glucose, obtain microbial inoculum by lyophilization, and moisture is less than 10%.
Beneficial effect:
The present invention, by demonstration and laying hen laboratory screening repeatedly, develops with Semen Maydis, Semen Tritici aestivi, Zein powder, bean cake, certain herbaceous plants with big flowers
The flower dregs of rice, corn byproducts, Oleum Glycines, enzyme preparation, microorganism formulation, vitamin E, organic selenium, organic chromium and organic zinc etc. are
The feedstuff of primary raw material；Repetition test finds out the feeding method of appropriate trophic level simultaneously, is divided into two stages: get rid of the hat phase 16
Week old 2% laying rate and peak early stage 2% laying rate 28 week old of laying eggs, and two stage design trophic levels are different, former
Material use has certain difference, fully meets organ of multiplication growth, body weight growth, lay heavily demand.Change conventional indistinction
Feeding method, is effectively increased feeding effect.
Science of the present invention compounds bacillus subtilis microbial agent, lactobacillus rhamnosus microbial inoculum and saccharomyces cerevisiae microbial inoculum, lactobacillus rhamnosus
Microbial inoculum and saccharomyces cerevisiae microbial inoculum have been respectively adopted CGMCC4430 and CGMCC4429.Product of the present invention by bacillus subtilis,
Saccharomyces cerevisiae microbial inoculum and lactobacillus rhamnosus microbial inoculum achieve organic assembling, at chicken gastric, due under the effect of body temperature factor, and enzyme
Preparation starts to have an effect, and decomposes wherein nutrient substance；Lactobacillus rhamnosus body fluid and around trophic factors is (especially after digestion
It is glucose) start growth and breeding and produce lactic acid, the distinctive strong galactopoiesis acidity of lactobacillus rhamnosus is played, the product of lactic acid
The raw digestive environments effectively promoting and being formed stomach, promotes digesting and assimilating of nutrient substance；Product bacillus subtilis of the present invention
Can effectively reduce antibiotics usage quantity in letting animals feed Deng probiotics factor entrance intestinal, improve letting animals feed
Immunity, improves the safety of animal meat product, improves the food utilization efficiency of animal, reduces antibiotic and medicine during routine is raised
The use of product, strengthens the resistivity of animal, improves raise benefit.
The target that the present invention reaches is to fully ensure that laying hen organ of multiplication is grown the most rapidly, and body weight increases fast, it is ensured that body constitution is strong,
Take a firm foundation for stable high yield；It is in particular in and gets rid of hat together, lay heavily.
Getting rid of the neat i.e. hen secondary sex characteristics cockscomb of hat to grow and red look fast soon, what wattle was the reddest looks fast soon, and whole chicken group's cockscomb is fast
Speed reddens greatly, and growing with organ of multiplication has medium above dependency soon and well.The initial stage egg size that reaches to lay eggs is big, height of laying eggs
Fast on peak, lay heavily effect；The present invention grows at initial stage largest body weightening finish of laying eggs, maximum organ of multiplication, it is ensured that body deposit
(energy and calcium phosphorus), the peak that makes to lay eggs maintains persistently, and reach more than 7 months to lay eggs peak, and 72 week old are laid eggs and reached 308 pieces
Egg, egg production more than 19.5 kilograms, produce a desired effect.
The present invention uses science compound feed, coordinates the organic assembling of accurate scale of feeding, fully ensures that laying hen organ of multiplication is the most fast
Speed is grown, and body weight increases fast, it is ensured that body constitution is strong, body maturation growth Tong Bus with sexual maturity, thus reach to open lay eggs great, height
Fast on peak, on height, and maintain peak of laying eggs lasting.Reach effect 28 average egg weight at weekend 61.3 grams, compare average level
Improve 3.03%；Laying rate reaches more than 95%；Egg production, from 48.2 grams/only/day, brings up to 50.1 grams/only/day；16 week old
28 week old stage death rates are reduced to 0.75%, and every chicken lays eggs 3 pieces more, 10,000 chicken many creation economic benefits 1.8 ten thousand yuan.
It is an object of the present invention to fully ensure that laying hen organ of multiplication is grown the most rapidly, body weight increases fast, it is ensured that body constitution is strong, for
Stable high yield is taken a firm foundation；It is in particular in and gets rid of hat together, lay heavily.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is this
Method well known to skilled person.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention,
The spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, real without departing substantially from the present invention
On the premise of matter and scope, the various changes or the change that carry out the material component in these embodiments and consumption fall within this
Bright protection domain.
Lactobacillus provided by the present invention is lactobacillus rhamnosus (Lactobacillus rhamnosus), and it is micro-that this bacterial strain is preserved in China
Biological inoculum preservation administration committee's common micro-organisms center, deposit number is CGMCC No.4430, preservation address: Beijing
North Star West Road, Chaoyang District 1 institute 3, Institute of Microorganism, Academia Sinica, postcode 100101.Preservation date 2010 12
The moon 08.
This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is shaft-like, and width is less than 1 μm, and 2 to 3 bacillus are prone to
It is linked to be and links together；On solid medium, this bacterium bacterium colony is milky, smooth surface, and moistening, thickness, edge is more whole
Together.Compared with original bacteria, this mutagenic strain is morphologically significantly less than starting strain.
It is the most micro-that starting strain lactobacillus rhamnosus CGMCC No.1.2134 is purchased from China Committee for Culture Collection of Microorganisms
Bio-Centers.
Lactobacillus rhamnosus of the present invention uses following flow process to carry out selection-breeding:
The original strain that sets out → test tube activation → high temperature acclimation → dithyl sulfate (DES) mutation → height sugar plate screening
→ nitrosoguanidine (NTG) mutagenesis screening → high temperature bacterium screening → shaking flask sieves → mitotic stability test → 5L fermentation again
Tank is tested
Bacterial strain CGMCC No.4430 hereditary stability result shows: through continuous passage ten times, property indices all compares surely
Fixed, heritability is preferable, and character is not replied, the purpose bacterial strain therefore bacterial strain CGMCC No.4430 obtained as selection-breeding.
Purpose bacterial strain CGMCC No.4430 does the experiment of 5L lactic acid fermentation tank, and result shows: compared with starting strain, CGMCC
No.4430 glucose-tolerant concentration can reach 270g/L, improves 95 parts compared with original bacteria；After fermentation ends, lactic acid content
For 60g/L, improve 158% compared with original bacteria.
Saccharomyces cerevisiae provided by the present invention (Saccharomyces cerevisiae), this bacterial strain is preserved in Chinese microorganism strain and protects
Hiding administration committee's common micro-organisms center, deposit number is CGMCC No.4429, preservation address: the Chaoyang District, Beijing City North Star
West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode 100101.Preservation date December in 2010 08 day.
This bacterial strain feature is as follows:
Examining under a microscope, the cell of this bacterial strain is avette, one end budding, and size is about 1 × 5 μm；On solid medium,
This bacterium bacterium colony is milky, smooth surface, and moistening, thickness, edge is more neat and medium bigger than normal.Compared with original bacteria, this lures
Become bacterial strain and be morphologically significantly less than starting strain.
Starting strain saccharomyces cerevisiae CICC31481 is purchased from Chinese industrial Microbiological Culture Collection administrative center.
Yeast strain of the present invention uses following flow process to carry out selection-breeding:
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutation → height oozes plate screening → nitrosoguanidine
(NTG) mutagenesis screening → height ooze flat board primary dcreening operation → shaking flask sieve again → mitotic stability test
The present invention first uses DES that starting strain is carried out mutation, oozes flat board (150g/L KCl) by beerwort height and cultivate after mutation
Base primary dcreening operation, then proceeds NTG mutation to the bacterial strain selected, and oozes flat board (200g/L KCl) by beerwort height and cultivates
Base primary dcreening operation, then uses 250mL triangular flask to sieve again, and then the yeast strain that selection-breeding is excellent is cooked passage assays, evaluate its heredity steady
Qualitative, and measure the metabolite content in fermentation liquid with liquid chromatograph, gas chromatograph, gas chromatography mass spectrometry.
Bacterial strain CGMCC No.4429 hereditary stability result shows: through continuous passage ten times, property indices all compares surely
Fixed, heritability is preferable, and character is not replied, the purpose bacterial strain therefore bacterial strain CGMCC No.4429 obtained as selection-breeding.
Purpose bacterial strain CGMCC No.4429 does the experiment of 7L fermentation tank, and result shows: compared with starting strain, CGMCC
No.4429 initial glucose tolerable concentration can reach 300g/L, improves 50% compared with original bacteria；After fermentation ends, residual sugar
It is 1g/L for 0.5g/L, glycerol content, improves 10% compared with original bacteria；Acetic acid content is 0.8g/L, lactic acid content is 0.4g/L,
Basic and original bacteria is suitable；Concentration of alcohol is 175g/L, improves 108% compared with original bacteria.
Bacillus subtilis CGMCC1.210, saccharomyces cerevisiae CGMCC4429, lactobacillus rhamnosus CGMCC4430.
Embodiment 1
(1) the most lay heavily 168 laying hen nutrition set meal application models of hat are got rid of
16 rejection crown materials, operational phase is 16 week old---2% laying rate, 1.6 tons/thousand chickens of scale of feeding.Described rejection crown material by with
The raw material composition of lower parts by weight:
Semen Maydis 54.365 parts, 3 parts of Testa oryzae, Testa Tritici 2.5 parts, bean cake 14 parts, fermented bean cake 3 parts, Semen arachidis hypogaeae dregs 3 parts, certain herbaceous plants with big flowers
The flower dregs of rice 2.8 parts, rapeseed meal 2 parts, DDGS3.5 part, corn bran 3 parts；1.0 parts of Oleum Glycines, stone powder 5.2 parts, calcium hydrogen phosphate
0.8 part, Sal 0.35 part, methionine 0.13 part, 70 lysine sulfate 0.12 part, probiotics 0.05 part, compound enzyme system
Agent 0.05 part, phytase 0.012 part, compound premix 1 part, choline chloride 0.1 part, yeast selenium 0.002 part；
Nutritive index is designed as metabolizable energy 2700 kcal/kg, crude protein 17.0%, apparent digestible lysine 0.72%, can digest egg
+ Guang 0.64%, calcium 2.2%, total phosphorus 0.55%.
8 peak early stage material, operational phase 2% laying rate-28 week old, scale of feeding is 8 tons/thousand chickens.
Described peak early stage material is made up of following parts by weight raw material:
Semen Maydis 55.945 parts, 2 parts of Testa oryzae, bean cake 15 parts, fermented bean cake 1.5 parts, Semen arachidis hypogaeae dregs 1 part, sunflower meal 3.1 parts,
Rapeseed meal 2.5 parts, DDGS2.7 part, corn bran 4 parts；1.0 parts of Oleum Glycines, stone powder 8.8 parts, Sal 0.35 part, methionine
0.12 part, 70 lysine sulfate 0.15 part, probiotics 0.05 part, compound enzymic preparation 0.05 part, phytase 0.012 part,
Compound premix 1 part, choline chloride 0.1 part, yeast selenium 0.002 part；
In described compound premix, the parts by weight content of each component is as follows:
Zinc gluconate 15 parts, Fe Lys 30 parts；Methionine chelate copper 2 parts；Copper chloride hydroxide 2 parts, aminoacid
Chelated Manganese 40 parts；Chromium picolinate 1.5 parts；Yeast selenium 1.5 parts；Calcium iodate 4 parts；Cobaltous chloride 0.5 part.Vitamin A accounts for
9 parts, vitamin D33 parts, vitamin E 11 parts, vitamin K3Account for 1 part, vitamin B10.4 part, vitamin B22 parts,
Vitamin B61.5 parts, vitamin B120.5 part, nicotiamide 16 parts, calcium pantothenate 4 parts, biotin 0.5 part.
In described compound enzymic preparation, the parts by weight content of each component is as follows: xylanase 2 parts, mannase 3 parts, albumen
Enzyme 3 parts, amylase 4 parts；
The enzyme activity unit of above-mentioned enzyme is as follows: xylanase 30000U/g, mannase 40000U/g, protease 5000U/g,
Amylase 6000U/g.
The parts by weight content of each component of described probiotics is as follows: bacillus subtilis microbial agent 2 parts, lactobacillus rhamnosus microbial inoculum 8
Part, saccharomyces cerevisiae microbial inoculum 2 parts.
Choline chloride: Zhong Xin bio tech ltd, Liaoning, main component choline, registered trade mark is sky wing.
Phytase: Beijing Smistyle Science & Technology Development Co., Ltd., main component phytase.Registered trade mark is sunrise ocean
Chroma-Pak: chromium comes U.S. 990, and a new Chemical Co., Ltd. of U.S. of Mianyang, Sichuan city, effective ingredient is chromium picolinate；
The preparation of bacillus subtilis microbial agent:
1. the acquisition of fermentation liquid: use slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid；
(1) first order seed is cultivated: bacillus subtilis slant strains accessed in 500 milliliters of shaking flasks, culture medium loading amount 100 milli
Rise, rotary shaker 180 revs/min, cultivation temperature 30 DEG C, incubation time 24 hours；
(2) secondary seed is cultivated: accesses in 500 milliliters of secondary seed shaking flasks by first order seed according to the inoculum concentration of 10%, cultivates
Condition is identical with first order seed；
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, culture medium
Loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 30 DEG C, incubation time 24 hours；
(4) first class seed pot is cultivated: with 10% inoculum concentration, three grades of seeds are accessed the total measurement (volume) first class seed pot as 150L, sends out
Ferment culture medium loading amount 100L, cultivation temperature 28 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa,
Incubation time 24 hours；
(5) fermentation culture: with 10% inoculum concentration, first class seed pot strain is accessed total measurement (volume) is 1.5 tons of secondary seed tanks, fermentation
Culture medium loading amount 1 ton, condition of culture cultivation temperature 28 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:0.5, tank pressure
0.05Mpa, incubation time 24 hours, cultivate and terminate bacteria concentration 5.0 × 108Individual/ml.
(6) centrifugation: use centrifuge to separate and obtain thalline.
(7) vacuum lyophilization: use drying process with atomizing to process after adding protective agent and obtain dry bacteria；Protective agent consists of
Defatted milk 10%, lactose 5%, glycerol 5%.Vacuum freeze-drying technique sees Institute of Microorganism, Academia Sinica and writes strain
Preservation handbook, publishes 610-653 in 1980.
Culture medium forms: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO3 1%, pH6.8.
The preparation method of lactobacillus rhamnosus agent:
(1) first order seed is cultivated: accessed by lactobacillus rhamnosus strain in 500 milliliters of shaking flasks, culture medium loading amount 100 milliliters,
Cultivation temperature 30 DEG C, incubation time 24 hours；
(2) secondary seed is cultivated: accesses in 500 milliliters of secondary seed shaking flasks by first order seed according to the inoculum concentration of 10%, cultivates
Condition is identical with first order seed；
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, culture medium
Loading amount 1000 milliliters, cultivation temperature 30 DEG C, incubation time 24 hours；
(4) first class seed pot is cultivated: with 5% inoculum concentration, three grades of seeds are accessed the total measurement (volume) first class seed pot as 150L, sends out
Ferment culture medium loading amount 100L, cultivation temperature 30 DEG C, tank pressure 0.05Mpa, incubation time 18 hours；
(5) fermentor cultivation: with 5% inoculum concentration, first class seed pot strain is accessed total measurement (volume) is 3 tons of secondary seed tanks, fermentation
Culture medium loading amount 2 tons, condition of culture cultivation temperature 30 DEG C, tank pressure 0.05Mpa, incubation time 24 hours, cultivate and terminate bacteria concentration
4.0 × 108Individual/ml.
(6) centrifugation: use centrifuge to separate and obtain thalline.
(7) vacuum lyophilization: adding protective agent and use vacuum freezing to be dried process technique, protective agent consists of defatted milk
10%, trehalose 5%, glycerol 5% and the aqueous solution of glucose 5%.Vacuum freeze-drying technique sees Chinese Academy of Sciences's microorganism
Institute writes culture presevation handbook, 1980.610-653.
Culture medium consists of: casein peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate
0.5%, citric acid diamidogen 0.2%, Tween 80 0.1%, K2HPO40.2%, MgSO4.7H2O 0.02%, MnSO4.H2O
0.005%, CaCO32%, agar 1.5%, pH6.8.
Bacillus subtilis CGMCC1.210, saccharomyces cerevisiae CGMCC4429, lactobacillus rhamnosus CGMCC4430；
(2) preparation method: after above-mentioned raw materials by weight feeding, be sufficiently mixed, reach between granularity 1.0-2.5mm
More than 50%.Then granule and probiotics are mixed.
(3) using method: 16 week old start to use when 20-30% cockscomb reddens rejection crown material, every thousand chicken scales of feeding 1.6 tons,
Lay eggs peak early stage material to starting transition during laying rate 2%, transition 6 days, feed 28 week old, before every thousand chickens feed peak
Phase expects 8 tons.
Use above-mentioned case study on implementation gets rid of rejection crown material and peak early stage in the most lay heavily 168 laying hen nutrition set meal application models of hat
Material, feeds according to set meal application model, does following checking:
(1) test:
Test and within 29th, carry out at Huan Ren Xinhua chicken house in JIUYUE in 2011 December on the 22nd.Matched group is peak material of commonly laying eggs,
Test group gets rid of rejection crown material and peak early stage material in the most lay heavily 168 laying hen nutrition set meal application models of hat for feeding the present invention.
Test chicken is grouped: test uses single factor experiment design, and experiment material selects 1152 blue brown 126 age in days laying hens in sea, point
Being two groups, often group sets 6 repetitions, 96 chickens of each repetition；
Fed control group and feedstuff group of the present invention respectively.
Feeding method: free choice feeding；Matched group, normally feeds according to market ordinary circumstance, the most stage by stage；Test group 16 week old
2% laying rate feeds 1.6 tons/thousand chickens of rejection crown material, all changes peak early stage material of laying eggs into 2% laying rate, during to 28 week old
Every thousand chickens feed 8 tons of peak early stage material of laying eggs.
Test temperature: using longitudinal ventilation, hen house temperature controls at 20-25 DEG C.Strictly control according to the blue brown feeding and management in sea；
Testing index includes: body weight start and at the end of twice, feed intake, survival rate.Laying rate, egg size, feedstuff-egg ratio,
Egg Quality and quality of egg etc., the results are shown in Table 1, table 2.
Table 1 present invention impact on laying hen expected date of confinement production performance
Table 2 present invention impact on laying hen 2% laying rate 28 week old egg laying performance
Note: colleague's shoulder marking-up mother's difference represents significant difference (P ＜ 0.05).
From Tables 1 and 2 result of the test it will be seen that get rid of hat phase and peak early stage of laying eggs, feedstuff of the present invention is at egg size, product
The aspects such as egg rate, feedstuff-egg ratio, feed intake and survival rate have good behaviour, are all better than common material group, have absolutely proved the present invention
The science of product and advance；Test shows: test group reduces by 75.3% than matched group antibiotic dosage.Use 10 points simultaneously
The hair color of chicken is given a mark by standard processed, and marking value is 9.6 points, and matched group is 7.3 points.Result shows that product of the present invention can improve
Body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.
Claims (5)
1. feed during getting rid of neat lay heavily laying hen 16 week old-2% laying rate rejection crown material of hat, 16 week old-2% laying rate, every 1000
Chicken is accumulative uses about 1.6 tons；Described rejection crown material is made up of the raw material of following parts by weight:
Semen Maydis 54.365 parts, 3 parts of Testa oryzae, Testa Tritici 2.5 parts, bean cake 14 parts, fermented bean cake 3 parts, Semen arachidis hypogaeae dregs 3 parts, certain herbaceous plants with big flowers
The flower dregs of rice 2.8 parts, rapeseed meal 2 parts, DDGS3.5 part, corn bran 3 parts；1.0 parts of Oleum Glycines, stone powder 5.2 parts, calcium hydrogen phosphate
0.8 part, Sal 0.35 part, methionine 0.13 part, 70% lysine sulfate 0.12 part, probiotics 0.05 part, compound enzyme system
Agent 0.05 part, phytase 0.012 part, compound premix 1 part, choline chloride 0.1 part, yeast selenium 0.002 part；
The parts by weight content of each component of described probiotics is as follows: bacillus subtilis microbial agent 1-3 part, lactobacillus rhamnosus microbial inoculum
6-10 part, saccharomyces cerevisiae microbial inoculum 1-3 part；
Described bacillus subtilis is CGMCC1.210, and saccharomyces cerevisiae is CGMCC4429, and lactobacillus rhamnosus is
CGMCC4430；
In described compound premix, the parts by weight content of each component is as follows:
Zinc gluconate 15 parts, Fe Lys 30 parts, methionine chelate copper 2 parts, copper chloride hydroxide 2 parts, aminoacid
Chelated Manganese 40 parts, chromium picolinate 1.5 parts, yeast selenium 1.5 parts, calcium iodate 4 parts, cobaltous chloride 0.5 part, vitamin A 9
Part, vitamin D33 parts, vitamin E 11 parts, vitamin K31 part, vitamin B10.4 part, vitamin B22 parts, dimension
Raw element B61.5 parts, vitamin B120.5 part, nicotiamide 16 parts, calcium pantothenate 4 parts, biotin 0.5 part.
Rejection crown material the most according to claim 1, it is characterised in that in described compound enzymic preparation, the parts by weight content of each component is such as
Under: xylanase 2-3 part, mannase 1-4 part, protease 3-4 parts, amylase 3-5 part.
Rejection crown material the most according to claim 1, it is characterised in that the preparation method of described lactobacillus rhamnosus microbial inoculum is as follows:
Slant culture lactobacillus rhamnosus seed liquor is transferred in fermentation tank, controls temperature and is 28-32 DEG C, Anaerobic culturel 22 hours,
Ventilation is 2.0m3/ minute；Complete centrifugation of fermenting obtain wet thallus, add consist of 10% defatted milk, 5% trehalose,
5% glycerol and the protective agent of 5% glucose, obtain microbial inoculum by lyophilization, and moisture is less than 10%.
Rejection crown material the most according to claim 1, it is characterised in that the preparation method of described bacillus subtilis microbial agent is as follows:
From inclined-plane, bacillus subtilis is cultivated in switching, and the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature and is
28-30 DEG C, aerlbic culture 19-24 hour, ventilation is 2.0m3/ minute；Complete centrifugation of fermenting obtains wet thallus, adds
Protective agent lyophilization obtains microbial inoculum, and protective agent consists of 10% defatted milk, 5% lactose, 5% glycerol.
Rejection crown material the most according to claim 1, it is characterised in that the preparation method of described saccharomyces cerevisiae microbial inoculum is as follows:
Slant strains through conventional multistage spread cultivation technique obtain yeast starter liquid, transfer in fermentation tank, control temperature be 28 DEG C, front
Phase aerlbic culture 14 hours, ventilation controls as 2.0m3/ minute, later stage Anaerobic culturel 15 hours；Fermentation liquid cryoconcentration,
After mixing with carrier, preparing through fluid bed drying, vehicle group becomes CaCO340 parts, 20 parts of dextrin, Zein powder 20
Part.
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| CN201310061331.1A CN103250678B (en) | 2013-02-25 | 2013-02-25 | Uniform-crowning high-egg-yield laying hen nutritional set feed application method and feed thereof |
| CN201410354307.1A CN104171643B (en) | 2013-02-25 | 2013-02-25 | Get rid of neat lay heavily laying hen 16 week old-2% laying rate rejection crown material of hat |
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| CN105432954B (en) * | 2015-10-30 | 2020-01-14 | 辽宁禾丰牧业股份有限公司 | Granularity-adjustable laying hen feed and preparation method thereof |
| CN107484914B (en) * | 2017-09-14 | 2021-02-02 | 四川圣迪乐村生态食品股份有限公司 | Laying hen nutrition enhancer for shortening peak time from laying to egg laying, preparation method and application |
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| CN101606634B (en) * | 2009-07-21 | 2012-03-21 | 辽宁众友饲料有限公司 | High-efficacy environment-friendly compound feed for layer hens |
| CN102028111B (en) * | 2010-09-06 | 2013-02-06 | 济南和美华动物营养品有限公司 | 817 miscellaneous chicken feed |
| CN102415506A (en) * | 2011-10-19 | 2012-04-18 | 辽宁禾丰牧业股份有限公司 | Expected delivery date compound feed for improving breeding performance |
| CN102318781B (en) * | 2011-10-20 | 2014-06-25 | 辽宁禾丰牧业股份有限公司 | Special laying hen later-period compound feed for improving commodity egg rate |
| CN102488113B (en) * | 2011-12-15 | 2013-05-22 | 天津工业大学 | Microbial feed additive |
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