CN104161768A - Veterinary compound tilmicosin injection and preparation method thereof - Google Patents
Veterinary compound tilmicosin injection and preparation method thereof Download PDFInfo
- Publication number
- CN104161768A CN104161768A CN201310391361.9A CN201310391361A CN104161768A CN 104161768 A CN104161768 A CN 104161768A CN 201310391361 A CN201310391361 A CN 201310391361A CN 104161768 A CN104161768 A CN 104161768A
- Authority
- CN
- China
- Prior art keywords
- injection
- tilmicosin
- preparation
- veterinary
- florfenicol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a veterinary compound tilmicosin injection and a preparation method thereof. 100ml of the veterinary compound tilmicosin injection comprises 15-30g of tilmicosin, 4-10g of florfenicol, 0.5-1.0g of an anti-oxidant, 3.3-8.0g of a cosolvent and the balance injection water. The veterinary compound tilmicosin injection having stable performances is prepared from tilmicosin and florfenicol as main raw materials and the anti-oxidant and the cosolvent as auxiliary materials. After intramuscular injection, the veterinary compound tilmicosin injection can be easily absorbed by animals and even completely absorbed, improves bioavailability, has long drug active ingredient action time, can be used for clinical treatment on livestock and poultry respiratory tract system disease caused by a plurality of pathogenic bacteria, has good curative effects and is convenient for use. The preparation method of the veterinary compound tilmicosin injection has simple processes, is convenient for operation and is suitable for large-scale industrial production.
Description
Technical field
The invention belongs to veterinary drug technical field, relate to a kind of animal compound tilmicosin injection, also relate to a kind of preparation method of animal compound tilmicosin injection simultaneously.
Background technology
Tilmicosin is a kind of by the semisynthetic macrolide antibiotics of tylosin, is the special-purpose antibiotic of poultry, medicinal its phosphate.Tilmicosin was succeeded in developing the eighties in 20th century, and preparation has tilmicosin soluble powder, tilmicosin pre-mixing agent (20%), tilmicosin injection etc.Tilmicosin all has inhibitory action to gram positive bacteria, some gram negative bacteria, mycoplasma, spirillum etc.; Pleuropneumonia actinomycetes, pasteurellosis bacillus are had to the antibacterial activity stronger than tylosin, the clinical mastitis prevented and treated domestic animal pneumonia (being caused by infection such as Actinobacillus pleuropneumoniae, pasteurellosis bacillus, mycoplasmas), avian mycoplasmas disease and lactogenic animal etc. that are mainly used in.But, tilmicosin injection unstable properties in the market, animal absorbs not exclusively, and bioavailability is not high, and effective ingredient is short action time, and its curative effect can't reach instructions for use.
Summary of the invention
The object of this invention is to provide a kind of animal compound tilmicosin injection, animal easily absorbs, long action time, good effect.
Second object of the present invention is to provide a kind of preparation method of animal compound tilmicosin injection.
In order to realize above object, the technical solution adopted in the present invention is: a kind of animal compound tilmicosin injection, in every 100ml injection, contain tilmicosin 15～30g, florfenicol 4～10g, antioxidant 0.5～1.0g, cosolvent 3.3～8.0g, surplus is water for injection.
Described antioxidant is any one or its combination in sodium formaldehyde sulphoxylate, sodium sulfite, disodiumedetate (EDTA-2Na).
Described cosolvent is any one or the combination in benzoic acid, sodium benzoate, salicylic acid, sodium salicylate, para-amino benzoic acid.
A preparation method for above-mentioned veterinary tilmicosin injection, comprises the following steps:
1) get 30ml water for injection, add antioxidant, cosolvent, stirring and dissolving, obtains solution A;
2) in step 1) gained solution A, add tilmicosin, florfenicol, stirring and dissolving, obtains solution B;
3) solution B is settled to 100ml with water for injection, after mix homogeneously, filtration, sterilizing, obtain.
Described in step 3), be filtered into the filter membrane that aperture is 0.45 μ m and filter.
Sterilizing described in step 3) is steam sterilization.
The vapor (steam) temperature of described steam sterilization is 115 ℃, and sterilization time is 20min.
The use amount of animal compound tilmicosin injection of the present invention is 10～20mg/kg body weight, in the quality of tilmicosin sterling.
Animal compound tilmicosin injection of the present invention, take tilmicosin, florfenicol is primary raw material, is aided with antioxidant, cosolvent, makes the compound recipe tilmicosin injection of stable performance, after animal intramuscular injection, easily absorb and easily absorb completely, having improved bioavailability; Effective ingredient long action time, is clinically used for the treatment of the livestock and poultry respiratory systemic disease that various pathogens causes, good effect is easy to use.The preparation method of animal compound tilmicosin injection of the present invention, technique is simple, easy to operate, is applicable to large-scale industrial production.
The specific embodiment
Below in conjunction with the specific embodiment, the present invention is further illustrated.
Embodiment 1
The animal compound tilmicosin injection of the present embodiment, contains tilmicosin 20g, florfenicol 5g, sodium formaldehyde sulphoxylate 0.5g, sodium benzoate 3.5g in every 100ml injection, surplus is water for injection.
A preparation method for above-mentioned veterinary tilmicosin injection, comprises the following steps:
1) get 30ml water for injection, add 0.5g sodium formaldehyde sulphoxylate, 3.5g sodium benzoate, stirring and dissolving, obtains solution A;
2) in step 1) gained solution A, add 20g tilmicosin, 5g florfenicol, stirring and dissolving, obtains solution B;
3) solution B is settled to 100ml with water for injection, after mix homogeneously, through the membrane filtration of 0.45 μ m, ampoule subpackage, 115 ℃ of steam sterilization 20min, obtain.
Embodiment 2
The animal compound tilmicosin injection of the present embodiment, contains tilmicosin 25g, florfenicol 8g, sodium sulfite 0.7g, salicylic acid 5.0g in every 100ml injection, surplus is water for injection.
A preparation method for above-mentioned veterinary tilmicosin injection, comprises the following steps:
1) get 30ml water for injection, add 0.7g sodium sulfite, 5.0g salicylic acid, stirring and dissolving, obtains solution A;
2) in step 1) gained solution A, add 25g tilmicosin, 8g florfenicol, stirring and dissolving, obtains solution B;
3) solution B is settled to 100ml with water for injection, after mix homogeneously, through the membrane filtration of 0.45 μ m, ampoule subpackage, 115 ℃ of steam sterilization 20min, obtain.
Embodiment 3
The animal compound tilmicosin injection of the present embodiment, contains tilmicosin 30g, florfenicol 10g, disodiumedetate 1.0g, sodium salicylate 8.0g in every 100ml injection, surplus is water for injection.
A preparation method for above-mentioned veterinary tilmicosin injection, comprises the following steps:
1) get 30ml water for injection, add 1.0g disodiumedetate, 8.0g sodium salicylate, stirring and dissolving, obtains solution A;
2) in step 1) gained solution A, add 30g tilmicosin, 10g florfenicol, stirring and dissolving, obtains solution B;
3) solution B is settled to 100ml with water for injection, after mix homogeneously, through the membrane filtration of 0.45 μ m, ampoule subpackage, 115 ℃ of steam sterilization 20min, obtain.
Experimental example 1
This experimental example be take pig and embodiment 1～3 gained veterinary tilmicosin injection is carried out to animal clinical trial to check its curative effect as experimental subject.
The sick initial stage fervescence of certain cultivation factory pig, spirit is depressed, loss of appetite, occur asthma, dysphoria, indivedual mouth and nose flow out foam, mental weariness, refusing to eat is slow-witted vertical, walking does not rise unstable or sleepingly, occurs individually sitting position of dog gesture, sometimes occurs cough, the basal part of the ear, veutro and extremity etc. are located skin and are occurred reddish violet speckle, while pressing with hands, fade, sick just feces is dry, becomes dry excrement ball later.Cut open 2 pigs that die of illness of inspection, trachea and bronchus inner product has large quantity of fluid and foam, and lymph nodes of body as a whole is congested, enlargement, and whole body mucosa has hemorrhage speckle; Lung has ecchymosis, and with edema and edema due to disorder of QI, spleen has petechia, hepatohemia, and gallbladder enlargement, cyst wall thicken.Comprehensive clinical manifestation and pathological change, cause of disease inspection, be diagnosed as swine pasteurellosis.
Choose 1200 of above-mentioned sick pigs, be equally divided into 6 groups, 200 every group.Infecting matched group feeds by normal condition and drinks water, not administration; Test group 1～3 is injected respectively embodiment 1～3 gained veterinary tilmicosin injection, and injection volume is 10mg/kg body weight (in the quality of tilmicosin sterling), feeds with drinking-water situation with infecting matched group; Comparative example 1 and comparative example 2 are injected respectively commercially available tilmicosin injection, and (injection volume is 10mg/kg body weight, take the quality of tilmicosin sterling) and commercially available florfenicol injection (injection volume is 10mg/kg body weight, quality in florfenicol sterling), feed with drinking-water situation with infecting matched group.Experimental result is as shown in table 1:
The examination of curative effect result of table 1 experimental example 1
The sick pig of test group 1～3, use after embodiment 1～3 gained veterinary tilmicosin injection injection once, taking a turn for the better appears in symptom, through the continual cure of 3 days, as shown in table 1, swinery was almost recovered, cure rate is more than 91%, and administration future trouble ill domestic animal is without any Novel presentation, and after curing, swinery diet is normal, grows fine; Compare with comparative example 2 with comparative example 1, the cure rate of embodiment 1～3 gained veterinary tilmicosin injection is apparently higher than independent use tilmicosin or florfenicol injection, and have advantages of onset rapidly, short treating period, easy to use.
Experimental example 2
This experimental example be take chicken and embodiment 1～3 gained veterinary tilmicosin injection is carried out to animal clinical trial to check its curative effect as experimental subject.
Certain plant's fowl mass-sending is sick, and chickling nose liquid increases, and flows out slurry property and viscosity nose liquid, is just transparent water sample, and rear flavescence is denseer thick, and nostril, common one or both sides is stopped up, and sick chicken dyspnea, shakes the head again and again, sneeze; Cockscomb, wattle are blue, breathe rale, and night is more obvious; Spirit and appetite still can, later stage poor appetite or do not eat young chicken growth retardation; The sick chicken having sheds tears, eyelid swelled; Become the symptom of chicken substantially similar to young chicken, but relax; Sick chicken inappetence, torpescence,, there is tracheal rale at a many slow-witted vertical a corner, stream nose liquid and cough.Pathology cuts open the visible chicken chest of inspection and belly bladder film thickens, muddiness, has the nodositas focus of the cheesy exudate of yellowish white or hypertrophy on blister cavities or cyst membrane, and size is differed in size by Semen Sesami to Semen Glycines; Serious chronic disease chicken, hole mucosa inflammation now, conjunctival congestion, blepharoedema or the mutual adhesion of upper palpebra inferior, one or both sides ophthalmic has pus sample or cheesy exudate, and laying hen, is also shown in salpingitis.In conjunction with clinical pathology, change, be diagnosed as avian mycoplasmas and infect.
Choose 1200 of above-mentioned sick chickens, be equally divided into 6 groups, 200 every group.Infecting matched group feeds by normal condition and drinks water, not administration; Test group 1～3 is injected respectively embodiment 1～3 gained veterinary tilmicosin injection, and injection volume is 0.5ml/, feeds and the same matched group that infects of drinking-water situation; Comparative example 1 and comparative example 2 are injected respectively commercially available tilmicosin injection and commercially available florfenicol injection, and injection volume is 0.5ml/, feed and the same matched group that infects of drinking-water situation.Experimental result is as shown in table 1:
Table 2 embodiment 1～3 gained veterinary tilmicosin injection examination of curative effect result
The sick chicken of test group 1～3, use after embodiment 1～3 gained veterinary tilmicosin injection injection secondary, taking a turn for the better obviously appears in symptom, through the continual cure of 3 days, as shown in table 2, chicken group be almost recovered, cure rate is more than 91%, and after administration, ill fowl is without any Novel presentation, and after curing, chicken group diet is normal, grows fine; Compare with comparative example, the cure rate of embodiment 1～3 gained veterinary tilmicosin injection is apparently higher than independent use tilmicosin or florfenicol injection.
Claims (7)
1. an animal compound tilmicosin injection, is characterized in that: in every 100ml injection, contain tilmicosin 15～30g, florfenicol 4～10g, antioxidant 0.5～1.0g, cosolvent 3.3～8.0g, surplus is water for injection.
2. animal compound tilmicosin injection according to claim 1, is characterized in that: described antioxidant is any one or its combination in sodium formaldehyde sulphoxylate, sodium sulfite, disodiumedetate.
3. animal compound tilmicosin injection according to claim 1, is characterized in that: described cosolvent is any one or the combination in benzoic acid, sodium benzoate, salicylic acid, sodium salicylate, para-amino benzoic acid.
4. a preparation method for veterinary tilmicosin injection as claimed in claim 1, is characterized in that: comprise the following steps:
1) get 30ml water for injection, add antioxidant, cosolvent, stirring and dissolving, obtains solution A;
2) in step 1) gained solution A, add tilmicosin, florfenicol, stirring and dissolving, obtains solution B;
3) solution B is settled to 100ml with water for injection, after mix homogeneously, filtration, sterilizing, obtain.
5. the preparation method of veterinary tilmicosin injection according to claim 4, is characterized in that: described in step 3), be filtered into the filter membrane that aperture is 0.45 μ m and filter.
6. the preparation method of veterinary tilmicosin injection according to claim 4, is characterized in that: sterilizing described in step 3) is steam sterilization.
7. the preparation method of veterinary tilmicosin injection according to claim 6, is characterized in that: the vapor (steam) temperature of described steam sterilization is 115 ℃, and sterilization time is 20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310391361.9A CN104161768A (en) | 2013-08-30 | 2013-08-30 | Veterinary compound tilmicosin injection and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310391361.9A CN104161768A (en) | 2013-08-30 | 2013-08-30 | Veterinary compound tilmicosin injection and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104161768A true CN104161768A (en) | 2014-11-26 |
Family
ID=51905894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310391361.9A Pending CN104161768A (en) | 2013-08-30 | 2013-08-30 | Veterinary compound tilmicosin injection and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104161768A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106074375A (en) * | 2016-07-21 | 2016-11-09 | 塔里木大学 | A kind of animal compound tilmicosin and florfenicol solid lipid nano suspension injection |
| CN108853019A (en) * | 2018-08-27 | 2018-11-23 | 广东三水正大康畜牧发展有限公司 | Water-soluble Tilmicosin Florfenicol compound injection and preparation method and application |
| CN116172952A (en) * | 2023-04-21 | 2023-05-30 | 浙江大学 | Tilmicosin xylitol compound injection and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101983632A (en) * | 2010-10-11 | 2011-03-09 | 西北农林科技大学 | Compound Tilmicosin nanoemulsion antibacterial agent and preparation method thereof |
| CN102133213A (en) * | 2011-03-25 | 2011-07-27 | 湖北武当动物药业有限责任公司 | Veterinary compound florfenicol injection and preparation method thereof |
| CN102657607A (en) * | 2012-05-25 | 2012-09-12 | 鼎正动物药业（天津）有限公司 | Tilmicosin stabilizing agent and preparation method thereof |
-
2013
- 2013-08-30 CN CN201310391361.9A patent/CN104161768A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101983632A (en) * | 2010-10-11 | 2011-03-09 | 西北农林科技大学 | Compound Tilmicosin nanoemulsion antibacterial agent and preparation method thereof |
| CN102133213A (en) * | 2011-03-25 | 2011-07-27 | 湖北武当动物药业有限责任公司 | Veterinary compound florfenicol injection and preparation method thereof |
| CN102657607A (en) * | 2012-05-25 | 2012-09-12 | 鼎正动物药业（天津）有限公司 | Tilmicosin stabilizing agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 傅超美等: "《药用辅料学》", 31 October 2008, article "液体制剂用辅料", pages: 45-46 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106074375A (en) * | 2016-07-21 | 2016-11-09 | 塔里木大学 | A kind of animal compound tilmicosin and florfenicol solid lipid nano suspension injection |
| CN108853019A (en) * | 2018-08-27 | 2018-11-23 | 广东三水正大康畜牧发展有限公司 | Water-soluble Tilmicosin Florfenicol compound injection and preparation method and application |
| CN116172952A (en) * | 2023-04-21 | 2023-05-30 | 浙江大学 | Tilmicosin xylitol compound injection and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102973489B (en) | Florfenicol solid dispersoid and preparation method thereof | |
| CN101468032A (en) | Soluble powder for treating respiratory illness of livestock and poultry | |
| CN104161768A (en) | Veterinary compound tilmicosin injection and preparation method thereof | |
| CN101450067A (en) | Premixing agent for treating and preventing livestock and poultry respiratory disease | |
| CN105534912A (en) | High-stability valnemulin hydrochloride soluble powder and uses thereof | |
| CN105709081B (en) | Pharmaceutical composition for treating infectious rhinitis of chicken, application and feed thereof | |
| CN101966178A (en) | Composition for treating chicken proventriculitis and preparation method thereof | |
| CN101628109A (en) | Compound neomycin sulphate solution and preparation method thereof | |
| CN102813909B (en) | Compound doxycycline hydrochloride composition for preventing and treating colibacillosis and chronic respiratory disease of poultry as well as preparation method thereof | |
| CN103169652B (en) | Veterinary alkaline lincomycin injection as well as preparation method and use thereof | |
| CN101450061A (en) | Soluble powder for treating poultry bacterial infection | |
| CN102743407B (en) | Oral liquid for treating infectious air sacculitis of chicken and preparation method thereof | |
| CN102755337B (en) | Compound florfenicol injection and preparation method thereof | |
| CN104586875A (en) | Preparation method for compound tilmicosin enteric-coated granules | |
| CN107970272A (en) | The purposes of pithecellobium clypearia or extract in treatment and/or the bronchopneumonic medicine of auxiliary treatment livestock and poultry is prepared | |
| CN102847160B (en) | Compound quinolone injection for livestock, and preparation method thereof | |
| CN104188987A (en) | Formula and preparation method of veterinary injectant for preventing and treating endometritis | |
| CN103830254A (en) | Soluble powder for treating respiratory diseases of livestock and poultry | |
| CN106236758B (en) | Compound preparation for treating chicken respiratory tract bacterial and virus mixed infection and preparation method thereof | |
| CN101468030A (en) | Soluble powder for treating digestive system infection of livestock and poultry | |
| CN101112377A (en) | Medicinal composition for treating avian mixed infection and method for preparing the same | |
| CN101879179A (en) | Compound Ofloxacin solution and preparation method thereof | |
| CN103432153B (en) | Novel prescription of injection for treating infectious pleuropneumonia of livestock and preparation technology thereof | |
| CN104587445A (en) | Pharmaceutical composition for treating nephrosis caused by avian infectious bronchitis nephritis and application method of pharmaceutical composition | |
| CN105418704A (en) | Tilmicosin-saccharin compound and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141126 |