CN104152503A - 一种提高异养下微藻脂肪酸产率的方法 - Google Patents
一种提高异养下微藻脂肪酸产率的方法 Download PDFInfo
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- CN104152503A CN104152503A CN201410427641.5A CN201410427641A CN104152503A CN 104152503 A CN104152503 A CN 104152503A CN 201410427641 A CN201410427641 A CN 201410427641A CN 104152503 A CN104152503 A CN 104152503A
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract description 17
- 239000000194 fatty acid Substances 0.000 title abstract description 17
- 229930195729 fatty acid Natural products 0.000 title abstract description 17
- 150000004665 fatty acids Chemical class 0.000 title abstract description 17
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 37
- 229910052698 phosphorus Inorganic materials 0.000 claims description 37
- 239000011574 phosphorus Substances 0.000 claims description 37
- 241000195493 Cryptophyta Species 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 13
- 150000002632 lipids Chemical class 0.000 claims description 11
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 claims description 10
- 229960004642 ferric ammonium citrate Drugs 0.000 claims description 10
- 239000004313 iron ammonium citrate Substances 0.000 claims description 10
- 235000000011 iron ammonium citrate Nutrition 0.000 claims description 10
- LTUDISCZKZHRMJ-UHFFFAOYSA-N potassium;hydrate Chemical compound O.[K] LTUDISCZKZHRMJ-UHFFFAOYSA-N 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 9
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 7
- 240000009108 Chlorella vulgaris Species 0.000 claims description 7
- 235000007089 Chlorella vulgaris Nutrition 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 4
- 239000013543 active substance Substances 0.000 claims description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 230000001651 autotrophic effect Effects 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 24
- 229910052757 nitrogen Inorganic materials 0.000 description 12
- 229910021654 trace metal Inorganic materials 0.000 description 12
- 239000002028 Biomass Substances 0.000 description 9
- 238000011534 incubation Methods 0.000 description 6
- 238000012856 packing Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 239000002551 biofuel Substances 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 239000007789 gas Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical compound COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- WETWJCDKMRHUPV-UHFFFAOYSA-N acetyl chloride Chemical compound CC(Cl)=O WETWJCDKMRHUPV-UHFFFAOYSA-N 0.000 description 1
- 239000012346 acetyl chloride Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000011430 maximum method Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229940095102 methyl benzoate Drugs 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000020238 sunflower seed Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
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CN201410427641.5A CN104152503B (zh) | 2014-08-27 | 2014-08-27 | 一种提高异养下微藻脂肪酸产率的方法 |
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CN201410427641.5A CN104152503B (zh) | 2014-08-27 | 2014-08-27 | 一种提高异养下微藻脂肪酸产率的方法 |
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CN104152503A true CN104152503A (zh) | 2014-11-19 |
CN104152503B CN104152503B (zh) | 2017-05-10 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107201314A (zh) * | 2017-07-08 | 2017-09-26 | 东北师范大学 | 一种同时提高生物量和油脂含量的微藻异养培养方法 |
WO2021058695A1 (de) * | 2019-09-25 | 2021-04-01 | Subitec Gmbh | Verfahren und vorrichtung zur aufreinigung von raum- oder stadtluft unter bereitstellung mindestens einer wertstofffraktion |
CN113512567A (zh) * | 2021-06-29 | 2021-10-19 | 安徽师范大学 | 一种提高光异养体系微藻产油率的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103952313A (zh) * | 2013-12-31 | 2014-07-30 | 上海交通大学 | 一株淡水藻Chlorella sorokiniana HN01及其应用 |
-
2014
- 2014-08-27 CN CN201410427641.5A patent/CN104152503B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103952313A (zh) * | 2013-12-31 | 2014-07-30 | 上海交通大学 | 一株淡水藻Chlorella sorokiniana HN01及其应用 |
Non-Patent Citations (3)
Title |
---|
A. ROOPNARAIN ET AL: "Phosphorus limitation and starvation effects on cell growth and lipid accumulation in Isochrysis galbana U4 for biodiesel production", 《BIORESOURCE TECHNOLOGY》 * |
FEI-FEI CHU ET AL: "Phosphorus plays an important role in enhancing biodiesel productivity of Chlorella vulgaris under nitrogen deficiency", 《BIORESOURCE TECHNOLOGY》 * |
XIAO-FEI SHEN ET AL: "Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation", 《WATER RESEARCH》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107201314A (zh) * | 2017-07-08 | 2017-09-26 | 东北师范大学 | 一种同时提高生物量和油脂含量的微藻异养培养方法 |
CN107201314B (zh) * | 2017-07-08 | 2019-11-19 | 东北师范大学 | 一种同时提高生物量和油脂含量的微藻异养培养方法 |
WO2021058695A1 (de) * | 2019-09-25 | 2021-04-01 | Subitec Gmbh | Verfahren und vorrichtung zur aufreinigung von raum- oder stadtluft unter bereitstellung mindestens einer wertstofffraktion |
CN113512567A (zh) * | 2021-06-29 | 2021-10-19 | 安徽师范大学 | 一种提高光异养体系微藻产油率的方法 |
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Inventor after: Zeng Jianxiong Inventor after: Shen Xiaofei Inventor after: Xia Xiuyang Inventor after: Chu Feifei Inventor after: Yu Shijin Inventor after: Hu Hao Inventor before: Zeng Jianxiong Inventor before: Shen Xiaofei Inventor before: Chu Feifei Inventor before: Yu Shijin Inventor before: Hu Hao |
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Free format text: CORRECT: INVENTOR; FROM: CENG JIANXIONG SHEN XIAOFEI CHU FEIFEI YU SHIJIN HU HAO TO: CENG JIANXIONG SHEN XIAOFEI XIA XIUYANG CHU FEIFEI YU SHIJIN HU HAO |
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Granted publication date: 20170510 |