CN104145027A - 用于室温原位检测生物样品中的目标核酸的方法和试剂盒 - Google Patents

用于室温原位检测生物样品中的目标核酸的方法和试剂盒 Download PDF

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Publication number
CN104145027A
CN104145027A CN201280069016.7A CN201280069016A CN104145027A CN 104145027 A CN104145027 A CN 104145027A CN 201280069016 A CN201280069016 A CN 201280069016A CN 104145027 A CN104145027 A CN 104145027A
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CN
China
Prior art keywords
nucleic acid
target nucleic
sample
probe
degrees celsius
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Pending
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CN201280069016.7A
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English (en)
Chinese (zh)
Inventor
J·奥里驰-科斯塔
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CELLAY Inc
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CELLAY Inc
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Application filed by CELLAY Inc filed Critical CELLAY Inc
Priority to CN201510835512.4A priority Critical patent/CN105385757B/zh
Publication of CN104145027A publication Critical patent/CN104145027A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201280069016.7A 2011-12-12 2012-12-12 用于室温原位检测生物样品中的目标核酸的方法和试剂盒 Pending CN104145027A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510835512.4A CN105385757B (zh) 2011-12-12 2012-12-12 用于室温原位检测生物样品中的目标核酸的方法和试剂盒

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161569656P 2011-12-12 2011-12-12
US61/569,656 2011-12-12
PCT/US2012/069178 WO2013090386A2 (en) 2011-12-12 2012-12-12 Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201510835512.4A Division CN105385757B (zh) 2011-12-12 2012-12-12 用于室温原位检测生物样品中的目标核酸的方法和试剂盒

Publications (1)

Publication Number Publication Date
CN104145027A true CN104145027A (zh) 2014-11-12

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CN201280069016.7A Pending CN104145027A (zh) 2011-12-12 2012-12-12 用于室温原位检测生物样品中的目标核酸的方法和试剂盒
CN201510835512.4A Expired - Fee Related CN105385757B (zh) 2011-12-12 2012-12-12 用于室温原位检测生物样品中的目标核酸的方法和试剂盒

Family Applications After (1)

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Country Status (7)

Country Link
US (2) US9145584B2 (https=)
EP (1) EP2802671B1 (https=)
JP (1) JP2015505241A (https=)
CN (2) CN104145027A (https=)
BR (1) BR112014014307A2 (https=)
DK (1) DK2802671T3 (https=)
WO (1) WO2013090386A2 (https=)

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Publication number Priority date Publication date Assignee Title
CN107406877A (zh) * 2015-02-04 2017-11-28 博洛尼亚大学 用于加快杂交反应的添加剂
CN108779490A (zh) * 2016-01-08 2018-11-09 雅培分子公司 包含硫氰酸胍的杂交缓冲液

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US9145584B2 (en) 2011-12-12 2015-09-29 Cellay, Inc. Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
US20140161972A1 (en) * 2012-12-09 2014-06-12 National Sun Yat-Sen University Method for forming conductive film at room temperature
BR112016010354B1 (pt) 2013-11-07 2019-09-17 Cellay, Inc. Métodos e kits para detectar um carcinoma de próstata e prever prognósticos de doença para cânceres de próstata
US10577645B2 (en) * 2016-03-18 2020-03-03 Norgen Biotek Corp. Methods and kits for improving global gene expression analysis of human blood, plasma and/or serum derived RNA
CN109996875B (zh) * 2016-08-24 2023-11-28 注入技术公司 使用dtbp浓缩微生物或提取核酸的方法
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
KR20240094019A (ko) * 2019-09-23 2024-06-24 엘리먼트 바이오사이언스, 인크. 세포적으로 처리가능한 핵산 서열분석 방법
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids

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WO2006108627A1 (en) * 2005-04-14 2006-10-19 Merck Patent Gmbh Anti-egfr antibody therapy based on an increased copy number of the egfr gene in tumor tissues
CN101970451A (zh) * 2007-07-26 2011-02-09 塞雷公司 高度可见的染色体特异性探针及相关方法

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WO2006108627A1 (en) * 2005-04-14 2006-10-19 Merck Patent Gmbh Anti-egfr antibody therapy based on an increased copy number of the egfr gene in tumor tissues
CN101970451A (zh) * 2007-07-26 2011-02-09 塞雷公司 高度可见的染色体特异性探针及相关方法

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107406877A (zh) * 2015-02-04 2017-11-28 博洛尼亚大学 用于加快杂交反应的添加剂
CN107406877B (zh) * 2015-02-04 2021-06-18 博洛尼亚大学 用于加快杂交反应的添加剂
CN108779490A (zh) * 2016-01-08 2018-11-09 雅培分子公司 包含硫氰酸胍的杂交缓冲液

Also Published As

Publication number Publication date
CN105385757A (zh) 2016-03-09
US20150368701A1 (en) 2015-12-24
WO2013090386A3 (en) 2013-08-22
US9145584B2 (en) 2015-09-29
DK2802671T3 (en) 2018-11-05
EP2802671A2 (en) 2014-11-19
US20130149705A1 (en) 2013-06-13
CN105385757B (zh) 2020-04-03
WO2013090386A2 (en) 2013-06-20
EP2802671B1 (en) 2018-07-18
US10400275B2 (en) 2019-09-03
BR112014014307A2 (pt) 2019-09-24
JP2015505241A (ja) 2015-02-19

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Application publication date: 20141112