CN104145019A - 棉花植物事件a26-5以及用于其检测的引物和方法 - Google Patents
棉花植物事件a26-5以及用于其检测的引物和方法 Download PDFInfo
- Publication number
- CN104145019A CN104145019A CN201280001629.7A CN201280001629A CN104145019A CN 104145019 A CN104145019 A CN 104145019A CN 201280001629 A CN201280001629 A CN 201280001629A CN 104145019 A CN104145019 A CN 104145019A
- Authority
- CN
- China
- Prior art keywords
- cotton
- sequence
- seq
- primer
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title abstract description 8
- 240000002024 Gossypium herbaceum Species 0.000 title abstract description 5
- 235000004341 Gossypium herbaceum Nutrition 0.000 title abstract description 5
- 229920000742 Cotton Polymers 0.000 claims abstract description 91
- 230000009466 transformation Effects 0.000 claims abstract description 56
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 241000255967 Helicoverpa zea Species 0.000 claims abstract description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims description 25
- 108020004414 DNA Proteins 0.000 claims description 20
- 241000607479 Yersinia pestis Species 0.000 claims description 12
- 230000009261 transgenic effect Effects 0.000 claims description 12
- 238000009396 hybridization Methods 0.000 claims description 9
- 239000000523 sample Substances 0.000 claims description 9
- 239000012634 fragment Substances 0.000 claims description 7
- 238000012216 screening Methods 0.000 claims description 7
- 238000012408 PCR amplification Methods 0.000 claims description 5
- 239000000969 carrier Substances 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000013598 vector Substances 0.000 claims description 5
- 239000012472 biological sample Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 241000589158 Agrobacterium Species 0.000 claims description 3
- 239000012620 biological material Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 238000002372 labelling Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 238000013467 fragmentation Methods 0.000 claims 1
- 238000006062 fragmentation reaction Methods 0.000 claims 1
- 241000219146 Gossypium Species 0.000 abstract description 79
- 238000003780 insertion Methods 0.000 abstract description 5
- 230000037431 insertion Effects 0.000 abstract description 5
- 108091029865 Exogenous DNA Proteins 0.000 abstract description 3
- 210000000349 chromosome Anatomy 0.000 abstract description 3
- 239000003147 molecular marker Substances 0.000 abstract 1
- 239000013615 primer Substances 0.000 description 32
- 241000196324 Embryophyta Species 0.000 description 29
- 241000238631 Hexapoda Species 0.000 description 26
- 230000003321 amplification Effects 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 238000003556 assay Methods 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 230000004087 circulation Effects 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 230000010354 integration Effects 0.000 description 5
- 229930027917 kanamycin Natural products 0.000 description 5
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical class O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 238000002105 Southern blotting Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 108700019146 Transgenes Proteins 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 239000003973 paint Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000011536 extraction buffer Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 108010052418 (N-(2-((4-((2-((4-(9-acridinylamino)phenyl)amino)-2-oxoethyl)amino)-4-oxobutyl)amino)-1-(1H-imidazol-4-ylmethyl)-1-oxoethyl)-6-(((-2-aminoethyl)amino)methyl)-2-pyridinecarboxamidato) iron(1+) Proteins 0.000 description 1
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 241000511343 Chondrostoma nasus Species 0.000 description 1
- 241001148495 Cibotium barometz Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 235000009429 Gossypium barbadense Nutrition 0.000 description 1
- 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 101000957437 Homo sapiens Mitochondrial carnitine/acylcarnitine carrier protein Proteins 0.000 description 1
- 108700001097 Insect Genes Proteins 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102100038738 Mitochondrial carnitine/acylcarnitine carrier protein Human genes 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- 101710183280 Topoisomerase Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000004176 ammonification Methods 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- WOWHHFRSBJGXCM-UHFFFAOYSA-M cetyltrimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+](C)(C)C WOWHHFRSBJGXCM-UHFFFAOYSA-M 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 238000003200 chromosome mapping Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 101150095418 crop gene Proteins 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000012869 germination medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
- 229960001225 rifampicin Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- CMJCEVKJYRZMIA-UHFFFAOYSA-M thallium(i) iodide Chemical compound [Tl]I CMJCEVKJYRZMIA-UHFFFAOYSA-M 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 235000018322 upland cotton Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Insects & Arthropods (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2012/000673 WO2013170398A1 (zh) | 2012-05-16 | 2012-05-16 | 棉花植物事件a26-5以及用于其检测的引物和方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104145019A true CN104145019A (zh) | 2014-11-12 |
Family
ID=49582955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280001629.7A Pending CN104145019A (zh) | 2012-05-16 | 2012-05-16 | 棉花植物事件a26-5以及用于其检测的引物和方法 |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN104145019A (enrdf_load_stackoverflow) |
| IN (1) | IN2014MN02540A (enrdf_load_stackoverflow) |
| WO (1) | WO2013170398A1 (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103003429A (zh) * | 2012-05-16 | 2013-03-27 | 创世纪转基因技术有限公司 | 棉花植物事件a2-6以及用于其检测的引物和方法 |
| CN108699552A (zh) * | 2016-08-25 | 2018-10-23 | 创世纪种业有限公司 | 棉花事件n15-5以及用于其检测的引物和方法 |
| CN109929850A (zh) * | 2017-12-15 | 2019-06-25 | 中国种子集团有限公司 | 抗线虫棉花转化事件ghp10 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2933673T3 (es) | 2015-09-11 | 2023-02-13 | BASF Agricultural Solutions Seed US LLC | Variantes de HPPD y métodos de uso |
| MA44716A (fr) | 2016-04-20 | 2019-02-27 | Bayer Cropscience Lp | Événement élite ee-gh7, procédés et kits d'identification de cet événement dans des échantillons biologiques |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134981A (zh) * | 1995-12-28 | 1996-11-06 | 中国农业科学院生物技术研究中心 | 携带编码杀虫蛋白质融合基因的表达载体及其转基因植物 |
| WO2000006757A1 (en) * | 1998-07-31 | 2000-02-10 | Mycogen Plant Science, Inc. | Improved plant transformation process by scaffold attachment regions (sar) |
| CN1463175A (zh) * | 2001-06-11 | 2003-12-24 | 孟山都技术有限公司 | 棉花事件mon15985以及检测它的组合物和方法 |
| CN101679996A (zh) * | 2007-04-05 | 2010-03-24 | 拜尔生物科学公司 | 抗虫棉花植物及其鉴定方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5152600A (en) * | 1999-05-20 | 2000-12-12 | Board Of Regents Of The University Of Nebraska, The | Method for identifying components involved in signal transduction pathways in higher plants |
-
2012
- 2012-05-16 IN IN2540MUN2014 patent/IN2014MN02540A/en unknown
- 2012-05-16 CN CN201280001629.7A patent/CN104145019A/zh active Pending
- 2012-05-16 WO PCT/CN2012/000673 patent/WO2013170398A1/zh active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134981A (zh) * | 1995-12-28 | 1996-11-06 | 中国农业科学院生物技术研究中心 | 携带编码杀虫蛋白质融合基因的表达载体及其转基因植物 |
| WO2000006757A1 (en) * | 1998-07-31 | 2000-02-10 | Mycogen Plant Science, Inc. | Improved plant transformation process by scaffold attachment regions (sar) |
| CN1463175A (zh) * | 2001-06-11 | 2003-12-24 | 孟山都技术有限公司 | 棉花事件mon15985以及检测它的组合物和方法 |
| CN101679996A (zh) * | 2007-04-05 | 2010-03-24 | 拜尔生物科学公司 | 抗虫棉花植物及其鉴定方法 |
Non-Patent Citations (2)
| Title |
|---|
| BAO HONG ZHANG,ET AL: "Recent progress in cotton biotechnology and genetic engineering in China", 《CURRENT SCIENCE》 * |
| YU J.Z，等: "BV679272", 《EMBL》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103003429A (zh) * | 2012-05-16 | 2013-03-27 | 创世纪转基因技术有限公司 | 棉花植物事件a2-6以及用于其检测的引物和方法 |
| CN108699552A (zh) * | 2016-08-25 | 2018-10-23 | 创世纪种业有限公司 | 棉花事件n15-5以及用于其检测的引物和方法 |
| CN108699552B (zh) * | 2016-08-25 | 2022-07-29 | 创世纪种业有限公司 | 棉花事件n15-5以及用于其检测的引物和方法 |
| CN109929850A (zh) * | 2017-12-15 | 2019-06-25 | 中国种子集团有限公司 | 抗线虫棉花转化事件ghp10 |
| CN109929850B (zh) * | 2017-12-15 | 2022-08-19 | 中国种子集团有限公司 | 抗线虫棉花转化事件ghp10 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013170398A1 (zh) | 2013-11-21 |
| IN2014MN02540A (enrdf_load_stackoverflow) | 2015-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104830847B (zh) | 用于检测玉米植物dbn9936的核酸序列及其检测方法 | |
| CN104878091B (zh) | 用于检测玉米植物dbn9978的核酸序列及其检测方法 | |
| CN104878094B (zh) | 用于检测除草剂耐受性玉米植物dbn9888的核酸序列及其检测方法 | |
| CN104946631A (zh) | 玉米转化事件“双抗12-5”及其特异性鉴定方法 | |
| CN112273114B (zh) | 昆虫抗性和除草剂耐受性大豆事件pDAB9582.816.15.1 | |
| CN103003429B (zh) | 棉花植物事件a2‑6以及用于其检测的引物和方法 | |
| CN106086011B (zh) | 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法 | |
| KR102771580B1 (ko) | 대두 식물 dbn8002를 검출하기 위한 핵산 서열 및 이의 검출 방법 | |
| Ling et al. | Development of marker-free insect-resistant indica rice by Agrobacterium tumefaciens-mediated co-transformation | |
| BR112020014816A2 (pt) | Molécula de ácido nucleico, sonda, par de oligonucleotídeos iniciadores e kit ou microarranjo para detectar um evento de transformação de milho, produto, e, métodos para detectar um evento de transformação de milho, para reprodução de milho, para controlar uma população de pragas de lepidóptera, para eliminar pragas de lepidóptera e para reduzir dano de pragas de lepidóptera em milho. | |
| WO2020207125A1 (zh) | 用于检测玉米植物dbn9501的核酸序列及其检测方法 | |
| CN109971880B (zh) | 用于检测玉米植物dbn9508的核酸序列及其检测方法 | |
| CN111247255B (zh) | 用于检测大豆植物dbn8007的核酸序列及其检测方法 | |
| CN112852801A (zh) | 转基因玉米事件lp007-1及其检测方法 | |
| CN116574724B (zh) | 抗虫耐草甘膦转基因玉米事件kj1003及其检测方法 | |
| CN107090464B (zh) | 抗虫抗除草剂玉米转化事件及其创制方法和检测方法 | |
| CN114787389A (zh) | 用于检测大豆植物dbn8205的核酸序列及其检测方法 | |
| CN104878096B (zh) | 用于检测除草剂耐受性玉米植物dbn9868的核酸序列及其检测方法 | |
| CN104878092B (zh) | 用于检测玉米植物dbn9953的核酸序列及其检测方法 | |
| CN104145019A (zh) | 棉花植物事件a26-5以及用于其检测的引物和方法 | |
| US10626411B2 (en) | Gene for encoding Bacillus thuringiensis crystal proteins, and use thereof | |
| CN104830983B (zh) | 用于检测玉米植物dbn9968的核酸序列及其检测方法 | |
| JP2007527694A (ja) | ワタアブラムシ耐性遺伝子 | |
| CN104846084B (zh) | 用于检测玉米植物dbn9927的核酸序列及其检测方法 | |
| CN108018369B (zh) | 玉米转化事件zm2-104的创制、检测与应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 518048, the 308 tower of Oriental Pearl science and technology building, Sha Mo Industrial Zone, Shenzhen, Guangdong, Futian District, 4 Applicant after: Biocentury Seed Industry Co., Ltd. Address before: 518048, the 308 tower of Oriental Pearl science and technology building, Sha Mo Industrial Zone, Shenzhen, Guangdong, Futian District, 4 Applicant before: Biocentury Transgene Co., Ltd. |
|
| COR | Change of bibliographic data | ||
| AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20180713 |
|
| AD01 | Patent right deemed abandoned |