CN104137845A - Preparation for preventing and treating trichoderma and application method of preparation - Google Patents
Preparation for preventing and treating trichoderma and application method of preparation Download PDFInfo
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- CN104137845A CN104137845A CN201310713818.3A CN201310713818A CN104137845A CN 104137845 A CN104137845 A CN 104137845A CN 201310713818 A CN201310713818 A CN 201310713818A CN 104137845 A CN104137845 A CN 104137845A
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- trichoderma
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- preventing
- treating
- bacteriostatic agent
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Abstract
The invention provides a preparation for preventing and treating trichoderma and an application method of the preparation. The existing method in which the whole bottle of culture is completely abandoned once the trichoderma is discovered is changed and an appropriate bacteriostat is created, thiophanate methyl, carbendazim or chlorothalonil is diluted by 1000 times through distilled water, and then the solution can be sprinkled. The preparation prepared by use of thiophanate methyl, carbendazim or chlorothalonil is capable of effectively controlling the generation and immediate killing of the trichoderma completely and guaranteeing all-nutrient absorption and rapid growth of the cordyceps fungus. As a result, no trichoderma is generated in the whole culture process and the trichoderma can be killed immediately with total effects, and the cordyceps fungus can be protected. At last, the purposes of improving the yield and the merit factor of culturing the cordyceps militaris and increasing the content of the active ingredients of the cordyceps can be achieved.
Description
Denomination of invention: a kind of preparation and using method of preventing and treating Trichoderma
Technical field: the present invention relates to a kind of preparation and using method thereof of preventing and treating Trichoderma.Be applicable to technology and technique that the dark cultivation stage control of artificial culture Cordyceps militaris Trichoderma infects.
Technical background: utilize routine techniques to cultivate Cordyceps militaris, after inoculation and enter dark cultivation stage, until light cultivation stage, culture medium of Cordyceps militaris very easily infects Trichoderma.This Trichoderma starts and aweto fungus association, covers very soon aweto fungus growth district, finally replaces aweto fungus.Once there is this Trichoderma, will endanger the growth of aweto fungus, the lighter affects output capacity and the factor of merit of aweto fungus, and Chinese caterpillar fungus active constituent content, as low inferior in formed cordycepin content.In the time of conventional cultivation technique, once find to occur Trichoderma in incubator the way that often adopts whole bottle culture all to give up.This method has improved cultivation cost virtually, causes economic benefit not good enough.
Therefore, prevent and treat in time and kill Trichoderma, for improving, Cordyceps militaris cultivation effect is most important.
Goal of the invention: once change the way that existing discovery Trichoderma just adopts whole bottle culture all to give up; innovate a kind of suitable bacteriostatic agent of selecting; control in time and preparation and the using method thereof of effectively killing Trichoderma; reach in full incubation and do not produce Trichoderma and can entirely imitate and kill in time, can protect again aweto fungus not encroached on.Finally reach the object that improves output capacity, the factor of merit and the Chinese caterpillar fungus active constituent content of cultivating Cordyceps militaris.
Summary of the invention: the present invention is through the open paper with reference to about suppressing Trichoderma inhibitor.Adopt thiophanate methyl or carbendazim or stone bacterium clear, 1000 times of distilled water solutions of prepared and diluted, implement to spray.All can 100% effectively control Trichoderma and produced and kill in time, ensured full Nutrient Absorption and the Fast Growth of aweto fungus.Make the Cordyceps militaris yield rate of cultivating reach 90-92%, the factor of merit reaches 85-90%, and cordycepin content, by 0.08% left and right of traditional approach, reaches 0.12-0.18%.
Technical scheme of the present invention: the present invention divides three steps to implement technical scheme.
The first step, utilizes thiophanate methyl or carbendazim or stone bacterium clear, and 1000 times of distilled water solutions of prepared and diluted (as accompanying drawing) are stand-by.
Second step, completes after inoculation the enforcement sprinkling of culture base-material surface.
The 3rd step, dark cultivation stage afterwards, once find that Trichoderma generates, implements to spray to Trichoderma formation zone in time.
Described sprinkling adopts syringe hydrojet, must not adopt spray apparatus.
Tool of the present invention has the following advantages:
1. can effectively prevent and treat Cordyceps militaris and cultivate the infection of overall process Trichoderma.
2. the preparation adopting, pollution-free aweto fungus hidden danger.
3. once overcome the way that existing discovery Trichoderma just adopts whole bottle culture all to give up, reduced production cost.
4. can effectively improve the content of the active ingredient such as yield rate, the factor of merit and cordycepin that Cordyceps militaris cultivates.Be conducive to improve the producer's economic benefit.
Brief description of the drawings:
Accompanying drawing name: suppress Trichoderma preparation preparation schematic diagram;
In figure: being 1. thiophanate methyl or carbendazim or the clear dry powder of stone bacterium, is 2. distilled water (1000 times of amounts), is 3. liquid filled container, 4. for leaving standstill and homogeneous program.
Concrete enforcement example, and by reference to the accompanying drawings, the present invention is further described.
Embodiment:
First prepare bacteriostatic agent clear liquid.
The first step, gets thiophanate methyl or carbendazim or the clear dry powder of stone bacterium (as schemed 1.), is placed in glass container, add distilled water, dilute 1000 times (as schemed 2.), make the aqueous solution (as schemed 3.), stir, (as schemed 4.), for subsequent use.
This antibacterial liquid can be placed by normal temperature, and homogeneous and dissolving program are shorter, and matching while using also can.
This bacteriostatic agent is to using thing to limit without usage ratio.
Second step, with the bacteriostatic agent wiping incubator inwall preparing; Also can rinse bottle with bacteriostatic agent;
The 3rd step, packs the culture base-material preparing into incubator;
The 4th step, by the bacteriostatic agent sprinkling media surface preparing, is sprayed onto comprehensively;
The 5th step, generates district to the Trichoderma of timely discovery and adopts syringe to spray this bacteriostatic agent liquid;
Thereby make the present invention effectively reach aforesaid goal of the invention.
Claims (5)
1. prevent and treat preparation and the using method of Trichoderma for one kind.Comprise whole pharmaceutical formulations and using method.
2. a kind of preparation of preventing and treating Trichoderma as claimed in claim 1, is characterized in that selecting thiophanate methyl or carbendazim or stone bacterium clear, 1000 times of distilled water solution preparations of prepared and diluted.
3. as a kind of preparation of preventing and treating Trichoderma under claim 1,2 belongs to the aqueous solution that distilled water stirs.
4. a kind of preparation and using method of preventing and treating Trichoderma as claimed in claim 1 is with the bacteriostatic agent wiping incubator inwall preparing; Also can rinse bottle with bacteriostatic agent; Then pack the culture base-material preparing into incubator; Spray media surface with the bacteriostatic agent preparing again; The Trichoderma of timely discovery is generated to district adopts syringe to spray this bacteriostatic agent liquid.
5. the present invention includes but be not limited to preparation control Trichoderma preparation and use said preparation to prevent from suppressing in Cordyceps militaris cultivation the embodiment of Trichoderma method, as long as in essential scope of the present invention, variation, modification that above-described embodiment is done all will drop within the scope of claim of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310713818.3A CN104137845A (en) | 2013-12-23 | 2013-12-23 | Preparation for preventing and treating trichoderma and application method of preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310713818.3A CN104137845A (en) | 2013-12-23 | 2013-12-23 | Preparation for preventing and treating trichoderma and application method of preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104137845A true CN104137845A (en) | 2014-11-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310713818.3A Pending CN104137845A (en) | 2013-12-23 | 2013-12-23 | Preparation for preventing and treating trichoderma and application method of preparation |
Country Status (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155634A (en) * | 2017-05-20 | 2017-09-15 | 天津市东方中滨农业科技有限公司 | Fruiting bodies of cordyceps militaris growth period has additional nutrients and prevented the breeding method that miscellaneous bacteria is spread |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2409570A2 (en) * | 2010-06-29 | 2012-01-25 | Basf Se | Fungicidal mixtures based on pyrazolopyridine compounds |
| CN102342216A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Method by combining physical method and chemical method to improve quality of cordyceps militaris |
| CN102533564A (en) * | 2011-11-21 | 2012-07-04 | 滨州职业学院 | Method for screening bio-control trichoderma in corn seedling stage root rot period |
| WO2015011615A1 (en) * | 2013-07-22 | 2015-01-29 | Basf Corporation | Mixtures comprising a trichoderma strain and a pesticide |
-
2013
- 2013-12-23 CN CN201310713818.3A patent/CN104137845A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2409570A2 (en) * | 2010-06-29 | 2012-01-25 | Basf Se | Fungicidal mixtures based on pyrazolopyridine compounds |
| CN102342216A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Method by combining physical method and chemical method to improve quality of cordyceps militaris |
| CN102533564A (en) * | 2011-11-21 | 2012-07-04 | 滨州职业学院 | Method for screening bio-control trichoderma in corn seedling stage root rot period |
| WO2015011615A1 (en) * | 2013-07-22 | 2015-01-29 | Basf Corporation | Mixtures comprising a trichoderma strain and a pesticide |
Non-Patent Citations (5)
| Title |
|---|
| 张萍华: ""5种杀菌剂对灵芝菌丝及绿色木霉生长的影响"", 《浙江林学院学报》, vol. 1, no. 18, 30 March 2001 (2001-03-30) * |
| 朱婷婷等: ""不同杀菌剂对平菇及有害菌木霉的毒力测定"", 《安徽农业科学》, vol. 39, no. 3, 20 January 2011 (2011-01-20) * |
| 江英成: ""香菇杂菌绿色木霉室内抑制试验初探"", 《河北林果研究》, vol. 16, no. 1, 30 March 2001 (2001-03-30) * |
| 王建华等: ""五种杀菌剂对绿色木霉菌丝生长的影响"", 《河南科技学院学报（自然科学版）》, vol. 34, no. 3, 30 September 2006 (2006-09-30) * |
| 王进忠等: ""6种常用杀菌剂对木霉菌生长发育的影响"", 《中国农学通报》, vol. 21, no. 6, 30 June 2005 (2005-06-30), pages 308 - 311 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155634A (en) * | 2017-05-20 | 2017-09-15 | 天津市东方中滨农业科技有限公司 | Fruiting bodies of cordyceps militaris growth period has additional nutrients and prevented the breeding method that miscellaneous bacteria is spread |
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| PB01 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141112 |