CN103027081A - Artificial Chinese eaglewood forming agent - Google Patents
Artificial Chinese eaglewood forming agent Download PDFInfo
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- CN103027081A CN103027081A CN201210569056XA CN201210569056A CN103027081A CN 103027081 A CN103027081 A CN 103027081A CN 201210569056X A CN201210569056X A CN 201210569056XA CN 201210569056 A CN201210569056 A CN 201210569056A CN 103027081 A CN103027081 A CN 103027081A
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- chinese eaglewood
- aquilaria sinensis
- microorganisms
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- agalloch eaglewood
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Abstract
The invention relates to an artificial Chinese eaglewood forming agent which consists of microorganisms and kali salt solution according to a volume ratio of 1:2 to 1:32. The microorganisms are selected from the following materials:(1) wood flour of a wound surface of a wounded aquilaria sinensis trunk; (2) ground powder on which Chinese eaglewood is formed; (3) dust particles around aquilaria sinensis trees, which has radius of 0.5 to 1 m; and (4) soil around the aquilaria sinensis trees, which has radius of 0.5 to 1 m. The kali salt solution is KCl solution with concentration of 2 percent. The microorganisms have the following culture conditions: the inoculation amount is 1 to 2 percent; a swing bed has rotating speed of 100 to 150 rpm; the temperature is 30 to 35 DEG C; a potato dextrose bouillon culture medium with pH of 7.0 is adopted. A method for preparing the artificial Chinese eaglewood forming agent comprises the following steps : (1) after culturing the microorganisms which are from different sources for 48h, respectively obtaining bacterium suspension; and (2) mixing the obtained microorganism bacterium suspensions and the kali salt solution according to proportion to obtain the artificial Chinese eaglewood forming agent. According to the invention, the defense reaction of aquilaria sinensis is utilized, Chinese eaglewood forming rate of Chinese eaglewood is improved and Chinese eaglewood forming time is shortened.
Description
Technical field:
The present invention relates to the artificial Edgeworthia chrysantha agent of a kind of agalloch eaglewood field, be specifically related to a kind of microorganism in conjunction with the artificial Edgeworthia chrysantha agent field of sylvite.
Background technology:
Suspension culture of Aquilaria sinensis is unique plant origin that China produces famous and precious aromatics medicinal material agalloch eaglewood.Suspension culture of Aquilaria sinensis self can not form agalloch eaglewood, only produces defense reaction when being subject to extraneous invasion and attack, and the secretion resin forms agalloch eaglewood through long accumulation.At present, the method for man made perfume (or spice) mainly contains and utilizes natural fungi invasion trunk; Next is to bake with iron wire boring scald, fire, makes resin ooze out.
But existing man made perfume method has agalloch eaglewood and makes the low characteristics with becoming fragrant time length of fragrant rate.
Summary of the invention:
Patent purpose of the present invention is the deficiency for present stage agalloch eaglewood man made perfume method, and the artificial Edgeworthia chrysantha agent of a kind of agalloch eaglewood and preparation method are provided, and can improve the fragrant rate of making of agalloch eaglewood and shorten into the fragrant time.
The artificial Edgeworthia chrysantha agent of a kind of agalloch eaglewood is characterized in that: by microorganism and potassium salt soln by volume 1:2-1:32 form.The source of microorganism has: the dust granules around (1) suspension culture of Aquilaria sinensis tree in the radius 0.5-1m; (2) soil in the radius 0.5-1m around the suspension culture of Aquilaria sinensis trunk.Condition of culture is: inoculum concentration 1-2%, shaking speed 100-150rpm, 30-35 ℃, PDB medium.Potassium salt soln is 2% KCl solution.The preparation method after (1) cultivates 48h with the microorganism of separate sources, obtains respectively bacteria suspension; (2) the microbial bacteria suspension that obtains is mixed in proportion with potassium salt soln, and get final product.
The principle of the invention: alternately be injected in the suspension culture of Aquilaria sinensis plant corpus in conjunction with the form of sylvite with drop by from agalloch eaglewood, isolating microorganism, be injected into suspension culture of Aquilaria sinensis tree body with sylvite first, be changed to the microorganism injection after 4-5 hour, in proper order the time interval is alternately injected sylvite and microorganism injection, and the defense reaction by suspension culture of Aquilaria sinensis is accelerated suspension culture of Aquilaria sinensis tree Edgeworthia chrysantha speed.
Beneficial effect is:
The present invention utilizes the defense reaction of suspension culture of Aquilaria sinensis, improves making fragrant rate and shortening into the fragrant time of agalloch eaglewood.
Embodiment:
PDB medium and PDA flat-plate solid medium are given birth to the worker available from Shanghai; Relate to " % " among the embodiment and be the quality percent by volume.
Dust granules around the embodiment 1. suspension culture of Aquilaria sinensis tree in the radius 0.5-1m: collection method: layer overlay plastic film around the suspension culture of Aquilaria sinensis tree, occupy PDA flat-plate solid medium at film, behind the 24h, flat board is regained, put into constant incubator and cultivate 48h, after growing bacterium colony on the flat board, will there be the place of bacterium colony to scrape with transfer needle; In the access PDB medium, shaking table is cultivated 48h, obtains bacteria suspension; Condition of culture is: inoculum concentration 1-2%, shaking speed 100-150rpm, 30-35 ℃.Potassium salt soln is 2% KCl solution.The preparation method after (1) microorganism is cultivated 48h, obtains bacteria suspension; (2) the microbial bacteria suspension that obtains is mixed in proportion with potassium salt soln, namely by microorganism and potassium salt soln 1:2-1:32 by volume, make the artificial Edgeworthia chrysantha agent of agalloch eaglewood.On comparatively sturdy and root near trunk, the artificial Edgeworthia chrysantha agent of the agalloch eaglewood that makes is packed in the infusion bag of drop syringe, another is packed, and to enter concentration be 2% KCl solution, two syringe needles of drop syringe are put into respectively root hole, be injected into suspension culture of Aquilaria sinensis tree body with sylvite first, be changed to the artificial Edgeworthia chrysantha agent of agalloch eaglewood of the present invention after 4-5 hour, alternately inject sylvite and the artificial Edgeworthia chrysantha agent of agalloch eaglewood of the present invention with this time interval.
Soil around the embodiment 2 suspension culture of Aquilaria sinensis trunks in the radius 0.5-1m, collection method: adopt suspension culture of Aquilaria sinensis tree root soil on every side, after moisture volatilizes, soil is put into the conical flask that sterile water is housed, and add the bead that bacterium is crossed in death of monks or nuns, shaking table 30min, so that thalline effectively separates with soil, after the soil precipitation, the liquid of getting the clarification part is coated with flat board, wherein medium is PDA flat-plate solid medium, flat board is put into constant incubator cultivate 48h, after bacterium colony grows, will have the place of bacterium colony to scrape with transfer needle, put into PDB medium shaking table and cultivate 48h, obtain bacteria suspension.Condition of culture is: inoculum concentration 1-2%, shaking speed 100-150rpm, 30-35 ℃.Potassium salt soln is 2% KCl solution.The preparation method after (1) microorganism is cultivated 48h, obtains bacteria suspension; (2) the microbial bacteria suspension that obtains is mixed in proportion with potassium salt soln, namely by microorganism and potassium salt soln 1:2-1:32 by volume, make the artificial Edgeworthia chrysantha agent of agalloch eaglewood.On comparatively sturdy and root near trunk, the artificial Edgeworthia chrysantha agent of the agalloch eaglewood that makes is packed in the infusion bag of drop syringe, another is packed, and to enter concentration be 2% KCl solution, two syringe needles of drop syringe are put into respectively root hole, be injected into suspension culture of Aquilaria sinensis tree body with sylvite first, be changed to the artificial Edgeworthia chrysantha agent of agalloch eaglewood of the present invention after 4-5 hour, alternately inject sylvite and the artificial Edgeworthia chrysantha agent of agalloch eaglewood of the present invention with this time interval.
Embodiment 5
Press embodiment 1-2 and implement, above embodiment respectively needs 48 in suspension culture of Aquilaria sinensis tree, produces agalloch eaglewood after half a year.
Claims (4)
1. artificial Edgeworthia chrysantha agent of agalloch eaglewood is characterized in that: by microorganism and potassium salt soln by volume 1:2-1:32 form.
2. the as requested artificial Edgeworthia chrysantha agent of 1 described a kind of agalloch eaglewood, it is characterized in that: described microorganism has following source: the dust granules around (1) suspension culture of Aquilaria sinensis tree in the radius 0.5-1m; (2) soil in the radius 0.5-1m around the suspension culture of Aquilaria sinensis trunk.
3. the as requested artificial Edgeworthia chrysantha agent of 2 described a kind of agalloch eaglewood, it is characterized in that: the condition of culture of described microorganism is: inoculum concentration 1-2%, shaking speed 100-150rpm, 30-35 ℃, the potato glucose broth bouillon of pH7.0.
4. the as requested artificial Edgeworthia chrysantha agent of 1 described a kind of agalloch eaglewood, it is characterized in that: described potassium salt soln is 2% KCl solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210569056XA CN103027081A (en) | 2012-12-25 | 2012-12-25 | Artificial Chinese eaglewood forming agent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210569056XA CN103027081A (en) | 2012-12-25 | 2012-12-25 | Artificial Chinese eaglewood forming agent |
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| CN103027081A true CN103027081A (en) | 2013-04-10 |
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| CN201210569056XA Pending CN103027081A (en) | 2012-12-25 | 2012-12-25 | Artificial Chinese eaglewood forming agent |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651151A (en) * | 2013-11-21 | 2014-03-26 | 中国医学科学院药用植物研究所海南分所 | Fungus for promoting aquilaria plants to generate agilawood and application of fungus |
| CN104082058A (en) * | 2014-06-17 | 2014-10-08 | 傅京亮 | Bionic agilawood bearing method of natural agilawood |
| CN104823742A (en) * | 2015-05-20 | 2015-08-12 | 林励 | Compound and method for stimulating overall coagulation of aquilaria sinensis |
| CN105340601A (en) * | 2015-09-29 | 2016-02-24 | 中国医学科学院药用植物研究所海南分所 | Microorganism liquid infusion method-based agilawood induction method and application |
| CN105340602A (en) * | 2015-09-29 | 2016-02-24 | 中国医学科学院药用植物研究所海南分所 | Dot-like and/or dot-line-like aligawood producing method and application |
| CN105830765A (en) * | 2016-04-26 | 2016-08-10 | 唐文君 | Method for generating fragrance of agilawood through natural fungi |
| CN106967617A (en) * | 2017-03-23 | 2017-07-21 | 伍亭亦 | A kind of agalloch eaglewood Edgeworthia chrysantha endogenetic fungus separation and numerous use culture medium and preparation method thereof soon |
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| CN102511329A (en) * | 2011-12-13 | 2012-06-27 | 徐景辉 | Artificial induction agarwood production method from tree of Aquilaria sinensis (Lour.) Gilg by high-pressure injection |
| CN102577856A (en) * | 2012-01-20 | 2012-07-18 | 中国医学科学院药用植物研究所 | Infusion set for producing lignum aquilariae resinatum and production method of lignum aquilariae resinatum |
| CN102726224A (en) * | 2012-04-17 | 2012-10-17 | 唐文 | Agilawood producing on aquilaria senensis gilg trees by using stick inserting method |
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- 2012-12-25 CN CN201210569056XA patent/CN103027081A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511329A (en) * | 2011-12-13 | 2012-06-27 | 徐景辉 | Artificial induction agarwood production method from tree of Aquilaria sinensis (Lour.) Gilg by high-pressure injection |
| CN102577856A (en) * | 2012-01-20 | 2012-07-18 | 中国医学科学院药用植物研究所 | Infusion set for producing lignum aquilariae resinatum and production method of lignum aquilariae resinatum |
| CN102726224A (en) * | 2012-04-17 | 2012-10-17 | 唐文 | Agilawood producing on aquilaria senensis gilg trees by using stick inserting method |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651151A (en) * | 2013-11-21 | 2014-03-26 | 中国医学科学院药用植物研究所海南分所 | Fungus for promoting aquilaria plants to generate agilawood and application of fungus |
| CN103651151B (en) * | 2013-11-21 | 2015-05-27 | 中国医学科学院药用植物研究所海南分所 | Fungus for promoting aquilaria plants to generate agilawood and application of fungus |
| CN104082058A (en) * | 2014-06-17 | 2014-10-08 | 傅京亮 | Bionic agilawood bearing method of natural agilawood |
| CN104082058B (en) * | 2014-06-17 | 2016-02-10 | 傅京亮 | A kind of bio-imitability Edgeworthia chrysantha method of natural agilawood |
| CN104823742A (en) * | 2015-05-20 | 2015-08-12 | 林励 | Compound and method for stimulating overall coagulation of aquilaria sinensis |
| CN105340601A (en) * | 2015-09-29 | 2016-02-24 | 中国医学科学院药用植物研究所海南分所 | Microorganism liquid infusion method-based agilawood induction method and application |
| CN105340602A (en) * | 2015-09-29 | 2016-02-24 | 中国医学科学院药用植物研究所海南分所 | Dot-like and/or dot-line-like aligawood producing method and application |
| CN105830765A (en) * | 2016-04-26 | 2016-08-10 | 唐文君 | Method for generating fragrance of agilawood through natural fungi |
| CN106967617A (en) * | 2017-03-23 | 2017-07-21 | 伍亭亦 | A kind of agalloch eaglewood Edgeworthia chrysantha endogenetic fungus separation and numerous use culture medium and preparation method thereof soon |
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Application publication date: 20130410 |