CN104127439A - Natural composite amino acid raw material preparation method and natural composite amino acid raw material - Google Patents
Natural composite amino acid raw material preparation method and natural composite amino acid raw material Download PDFInfo
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Abstract
The invention discloses a natural composite amino acid raw material preparation method. The method comprises the following steps: 1, taking cryopreserved fresh pig brain, unfreezing, cleaning, homogenating, adding the above obtained slurry to concentrated hydrochloric acid, uniformly stirring, and centrifuging to obtain precipitated brain proteins; 2, adding the precipitated proteins to sulfuric acid, hydrolyzing, adjusting the pH value to 7.5-8.5, and filtering to obtain an acid hydrolysate; 3, heating the acid hydrolysate, adding active carbon, separating, collecting the above obtained supernatant, and adjusting the pH value to 2.5-4.5 to obtain a crude extract liquid; 4, adding the crude extract liquid to a cation exchange column, adjusting the pH value to 6.5-7.5, eluting with ammonia water, collecting the obtained eluate, concentrating to remove ammonia water, adjusting the pH value to 6.5-7.5, and preserving at -20DEG C to obtain a frozen liquid; and 5, naturally unfreezing the frozen liquid, centrifuging, removing tyrosine and related impurities, collecting the newly obtained supernatant, carrying out ultrafiltration on the supernatant, collecting the obtained filtrate, and drying to obtain the desired natural composite amino acid raw material.
Description
Technical field
The present invention relates to a kind of preparation method of natural compound amino-acid raw material, also relate to a kind of natural compound amino-acid raw material.
Background technology
Aminoacid (Amino acid) is the ultimate unit that forms protein, gives protein specific molecular morphosis, makes his molecule have biochemical activity.Protein is important bioactive molecule in organism, comprises the metabolic ferment of catalysis and enzyme.Aminoacid is to form organism protein and with vital movement the most basic relevant material, is the ultimate unit that forms in vivo protein molecule, has close relationship with biological vital movement.It has special physiological function in antibody, is one of indispensable nutritional labeling in organism.
Aminoacid roughly can be divided three classes:
1, essential amino acids: refer to that human body or other vertebrates can not be synthesized or aggregate velocity is far not suitable with the needs of body, must be supplied with by food proteins, these aminoacid are called essential amino acids.The requirement of adult's essential amino acids is about the 20-37% of protein requirement.Have 8 kinds, respectively: lysine, promote brain development, be the constituent of liver and gallbladder, can promote lipid metabolism, regulate pineal gland, mammary gland, corpus luteum and ovary, prevent cell degradation; Tryptophan: promote the generation of gastric juice and pancreatic juice; Phenylalanine: participate in eliminating the loss of kidney and bladder function; Methionine (methionine): participate in forming hemoglobin, tissue and serum, have the function that promotes spleen, pancreas and lymph; Threonine: some aminoacid that changes reaches the function of balance; Isoleucine: participate in adjusting and the metabolism of thymus, spleen and infracerebral gland; Infracerebral gland belongs to general headquarter and acts on thyroid, gonad; Leucine: effect balance isoleucine; Valine: act on corpus luteum, mammary gland and ovary.
2, semi-dispensable amino acid and conditionally essential amino acid
:arginine: the compound formulation that arginine and deoxycholic acid are made (Ming Nuofen) is to cure mainly the sick active drug such as syphilis, viral jaundice; Histidine: can be used as biochemical reagents and medicament, also can be used for treating heart disease, anemia, the medicine of rheumatic arthritis etc.Though human body can synthesize arginine and histidine, conventionally can not meet normal needs, therefore, be otherwise known as semi-dispensable amino acid or conditionally essential amino acid, be essential amino acids these two kinds of physical growth of children phases.Human body declines along with the increase at age to the requirement of essential amino acids, and adult is than the remarkable decline of baby.
3, non essential amino acid: refer to that people or other vertebrates oneself can be synthesized by simple precursor, the aminoacid that need to not obtain, aminoacid such as glycine, alanine from food.
If human body lacks any essential amino acids, just can cause physiological function abnormal, affect normally carrying out of organism metabolism, finally cause disease.Equally, if lack some non essential amino acid in human body, can produce organism metabolism obstacle.Arginine and citrulline are very important to forming carbamide; Cystine Deficiency of Intake will cause that insulin reduces, blood sugar increasing.And for example after wound, cystine and arginic requirement increase, as lacked, even heat energy abundance synthetic protein smoothly still.In a word, aminoacid can be brought into play more following effects by metabolism in human body: 1. synthetic tissue protein; 2. become acid, hormone, antibody, creatine etc. containing ammoniacal substance; 3. change carbohydrate and fat into; 4. be oxidized to carbon dioxide and water and carbamide, produce power.Therefore, the existence of aminoacid in human body, not only provides the important source material of synthetic protein, and for promoting growth, carry out homergy, sustaining life provides material base.If human body lacks or minimizing is wherein a certain, the normal life metabolism of human body will be subject to obstacle, even causes the generation of various diseases or vital movement to stop.So human body needs constantly to supplement aminoacid, some diseases need to rely on aminoacid treatment.
The normal aminoacid product using of people is non-natural Hausmam Amin 20 of synthetic now.This Hausmam Amin 20 has the human body protein of promotion Metabolism of Normal, corrects negative nitrogen balance, supplements protein, the effect of accelerating wound.Amino acid transfusion, the in the situation that of Power supply abundance, can enter histiocyte, participates in the anabolism of protein, obtains positive nitrogen balance, and generates enzyme, hormone, antibody, structural protein, promotes organization healing, recovers normal physiological function.But existing compounded amino acid product has very large side effect, as Amino Acid Compound Injection can cause the anaphylaxis of rash sample, once occur to discontinue medication; Occasionally have nauseating, vomiting, uncomfortable in chest, cardiopalmus, feel cold, heating or headache etc.
People are the security concern of existing non-natural compounded amino acid product.Those skilled in the art catch at and a kind ofly use safe compounded amino acid product, but never solve this technical problem.
Summary of the invention
The technical problem to be solved in the present invention is just to provide a kind of preparation method of natural compound amino-acid raw material, and this natural compound amino-acid raw material can be used in preparation and uses safe compounded amino acid product.A kind of natural compound amino-acid raw material is also provided.
In order to solve the problems of the technologies described above, the preparation method of natural compound amino-acid raw material of the present invention comprises the steps:
A. get frozen fresh Medulla sus domestica, thaw, clean, homogenate, adds serosity that concentrated hydrochloric acid stirs evenly, then centrifugalize, removes upper strata emulsion, and collecting precipitation thing, must precipitate brain albumen;
B. add sulphuric acid to be hydrolyzed the protein precipitation obtaining through above-mentioned steps, filter, obtain acid hydrolysis liquid;
C. by the acid hydrolysis liquid heating obtaining through above-mentioned steps, add active carbon, separation, collects supernatant, and adjust pH is 2.5-4.5, obtains crude extract;
D. by cation exchange column on the crude extract obtaining through above-mentioned steps, adjust pH is 6.5-7.5, uses ammonia eluting, collects eluent, concentrated deammoniation water, and adjust pH is 6.5-7.5,20 ℃ of preservations of ﹣, obtain freezing liquid;
E. will naturally thaw through above-mentioned steps gained freezing liquid, centrifugalize, removes tyrosine and related impurities, collects supernatant, by supernatant ultrafiltration, collects filtrate, dry, obtains required natural compound amino-acid raw material.
As further improvement, described a step, get frozen fresh Medulla sus domestica, soaking nature thaws, water cleans again, then the colloid mill homogenate 15min for purified water that adds the heavy 2-4 times of weight of Medulla sus domestica, homogenate gained serosity is poured into after being heated to 70-100 ℃ in extraction pot and is incubated 10-30min, in the heavy every 1kg of Medulla sus domestica, adding the ratio of 2-5 ml to add concentration is again that the concentrated hydrochloric acid of 12mol/L stirs evenly, is cooled to 10-50 ℃, again with the speed centrifugalize of 3000-4000r/min, then remove upper strata emulsion, collecting precipitation thing, must precipitate brain albumen.
As further improvement, described b step, by adding the ratio of 750-1500ml to add concentration through a step gained protein precipitation in every 1kg, be the sulphuric acid of 18.4mol/L, make the sulfuric acid solution that concentration is 6-12mol/L, be heated to 110-121 ℃, hydrolysis 6-9h, with weight ratio 5-10 water dilution doubly, with aqua calcis adjust pH, be 7.5-8.5 again, then use 200-300 order filter-cloth filtering, remove calcium sulfate precipitation thing, obtain acid hydrolysis liquid.
As further improvement, described c step, will be heated to 75-80 ℃ through b step gained acid hydrolysis liquid, the active carbon that adds again acid hydrolysis liquid weight 0.5-1%, is heated to 80-90 ℃, insulation 15min, while hot with the speed centrifugalize 5min of 3000-4000r/min, collecting supernatant, is 2.5-4.5 with oxalic acid solution adjust pH, standing 1-3h, get supernatant, precipitate with the speed centrifugalize 5min of 3000-4000r/min, is removed calcium oxalate precipitation thing again, merges supernatant and obtains crude extract.
As further improvement, described d step, will cross cation exchange column through c step gained crude extract, then with purified water rinse to pH value be 6.5-7.5, remove residue oxalic acid and calcium ion, then use the ammonia eluting of 1.8-2mol/L, collect eluent, tune pH value is 8-12, with per kilogram Medulla sus domestica, add the ratio of 1-3g to add sodium hydroxide concentrating under reduced pressure deammoniation water again, be concentrated into the amount of the heavy 1/5-1/3 of Medulla sus domestica, then with the hydrochloric acid of 6mol/L, adjust ph to 6.6-7.5, with 20 ℃ of ﹣, preserve 24h, obtain freezing liquid.
As further improvement, described e step, d step gained freezing liquid is thawed naturally, then with the speed centrifugalize of 3000-4000r/min, remove tyrosine and related impurities, collect supernatant, supernatant is carried out to ultrafiltration with 10000 Dalton molecular weight filter membranes, collect filtrate, then be drying to obtain natural compound amino-acid raw material with 60 ℃ of vacuum drying ovens of ﹣ or spraying.
Described natural compound amino-acid raw material is extracted and is obtained by pig brain tissue.
The natural compound amino-acid raw material of preparing according to method of the present invention, through fixed molten, by the stock solution of allocating after stock solution standard test content, through calibrating: character: for light yellow to brown color liquid; Differentiate: need testing solution chromatogram and reference substance solution chromatogram are basically identical; PH value is 6.5-7.5.Qi You pig brain tissue extracts and obtains, and contains 15 seed amino acids such as Aspartic Acid, serine, glutamic acid, glycine, histidine, arginine, threonine, alanine, proline, valine, methionine, lysine, isoleucine, leucine and phenylalanine.Through clinical trial, product prepared by natural compound amino-acid raw material of the present invention, overcome the defect of existing non-natural compound amino acid product, there is no the side effect of existing non-natural Hausmam Amin 20 completely, there is no other untoward reaction yet, safe and reliable, can be used in preparation and use safe compounded amino acid product to substitute existing non-natural compound amino acid product, to meet the need of market.
Natural compound amino-acid raw material of the present invention can be for the preparation of the various compounded amino acid products that use safety, as medical infusion solutions, injection, freeze-dried powder and oral formulations etc.; Also can be used for preparing health of masses food and health product, as additive, use etc., there is wide market prospect.
Method of the present invention can realize object of the present invention, can prepare natural compound amino-acid raw material for the various compounded amino acid products of production and application safety.
Method of the present invention, hydrochloric acid and sodium hydroxide use amount to sodium salt exist the content in stock solution few, after sulphuric acid hydrolysis, by calcium hydroxide and oxalic acid neutralisation of sulphuric acid, form calcium sulfate and calcium oxalate precipitation and remove partial impurities, through chromatography, can thoroughly remove calcium sulfate and calcium oxalate again, make 15 kinds of acid-hydrolyzed compounded amino acid starting materials of pure natural, refer to accompanying drawing.Method of the present invention is simple and reliable, cost is low, can a large amount of animal brains of disposable processing as Medulla sus domestica, during purification, applied sample amount is large, can carry out the large-scale production of natural compound amino-acid raw material, meets market demand.
Accompanying drawing explanation
Fig. 1 is the HPLC detection figure of 15 kinds of pure natural composite amino-acids of the inventive method gained.
The specific embodiment
Describe the embodiment of the inventive method below in detail, but be not limited only to following examples.
Embodiment mono-
The preparation method of this natural compound amino-acid raw material of the present invention comprises the steps:
A. get frozen fresh Medulla sus domestica 50kg, soaking nature thaws, water cleans again, then add colloid mill homogenate 15min for 100kg purified water, homogenate gained serosity is poured into after being heated to 80 ℃ in extraction pot and is incubated 15min, then add the concentrated hydrochloric acid that 250 ml concentration are 12mol/L to stir evenly, be cooled to 40 ℃, again with the speed centrifugalize of 3800r/min, then remove upper strata emulsion, collecting precipitation thing, must precipitate brain albumen 2.3kg;
B. by add 3000ml concentration through a step gained protein precipitation, be that the sulphuric acid of 18.4mol/L is hydrolyzed, make the sulfuric acid solution that concentration is 12mol/L, be heated to 121 ℃, hydrolysis 8h, again with the water dilution of 10 times of weight ratios, with aqua calcis adjust pH, be that 7.5(aqua calcis used adds the dissolving of 5000ml purified water with 2800g calcium hydroxide and obtains), then use 300 order filter-cloth filterings, remove calcium sulfate precipitation thing, obtain acid hydrolysis liquid;
C. decolouring, soon through b step gained acid hydrolysis liquid, be heated to 75 ℃, the active carbon that adds again acid hydrolysis liquid weight 1%, be heated to 80 ℃, insulation 15min, while hot with the speed centrifugalize 5min of 3800r/min, collect supernatant, with oxalic acid solution adjust pH, be that 3.5(oxalic acid solution used adds 2000ml purified water heating for dissolving with 750g oxalic acid and obtains), standing 3h, gets supernatant, and precipitate is again with the speed centrifugalize 5min of 3800r/min, remove calcium oxalate precipitation thing, merge supernatant and obtain crude extract;
D. will cross cation exchange column through c step gained crude extract, then with purified water rinse to pH value be 6.5, remove residue oxalic acid and calcium ion, then use the ammonia eluting of 1.8mol/L, collect eluent, adjusting pH value is 12, add again 150g sodium hydroxide concentrating under reduced pressure deammoniation water, be concentrated into the amount of 30g, then with the hydrochloric acid of 6mol/L, adjust ph to 7.2, with 20 ℃ of ﹣, preserve 24h, obtain freezing liquid;
E. d step gained freezing liquid is thawed naturally, then, with the speed centrifugalize of 3800r/min, remove tyrosine and related impurities, collect supernatant, supernatant is carried out to ultrafiltration with 10000 Dalton molecular weight filter membranes, collect filtrate, then spraying is drying to obtain natural compound amino-acid raw material.
Gained natural compound amino-acid raw material packet contains the aminoacid of following weight portion: Aspartic Acid 4.87, serine 2.08, glutamic acid 5.92, glycine 2.55, histidine 0.94, arginine 1.77, threonine 1.58, alanine 2.81, proline 2.42, valine 1.77, methionine 0.91, lysine 3.81, isoleucine 1.20, leucine 3.08, phenylalanine 1.24.15 seed amino acid total amounts 36.95.Through fixed molten, by the stock solution of allocating after stock solution standard test content, through calibrating: character: for light yellow to brown color liquid; Differentiate: need testing solution chromatogram and reference substance solution chromatogram are basically identical; PH value is 7.5.
Embodiment bis-
A. get frozen fresh Medulla sus domestica 50kg, soaking nature thaws, water cleans again, then add colloid mill homogenate 15min for 200kg purified water, homogenate gained serosity is poured into after being heated to 70 ℃ in extraction pot and is incubated 10min, then add the concentrated hydrochloric acid that 100 ml concentration are 12mol/L to stir evenly, be cooled to 10 ℃, again with the speed centrifugalize of 3000r/min, then remove upper strata emulsion, collecting precipitation thing, must precipitate brain albumen 2.4kg;
B. by add 2500ml concentration through a step gained protein precipitation, be that the sulphuric acid of 18.4mol/L is hydrolyzed, make the sulfuric acid solution that concentration is 6mol/L, be heated to 110 ℃, hydrolysis 6h, again with the water dilution of 5 times of weight ratios, with aqua calcis adjust pH, be that 8(aqua calcis used adds the dissolving of 5000ml purified water with 2500g calcium hydroxide and obtains), then use 200 order filter-cloth filterings, remove calcium sulfate precipitation thing, obtain acid hydrolysis liquid;
C. decolouring, soon through b step gained acid hydrolysis liquid, be heated to 75 ℃, the active carbon that adds again acid hydrolysis liquid weight 0.5%, be heated to 85 ℃, insulation 15min, while hot with the speed centrifugalize 5min of 3000r/min, collect supernatant, with oxalic acid solution adjust pH, be that 2.5(oxalic acid solution used adds 3000ml purified water heating for dissolving with 150g oxalic acid and obtains), standing 1h, gets supernatant, and precipitate is again with the speed centrifugalize 5min of 4000r/min, remove calcium oxalate precipitation thing, merge supernatant and obtain crude extract;
D. will cross cation exchange column through c step gained crude extract, then with purified water rinse to pH value be 7.5, remove residue oxalic acid and calcium ion, then use the ammonia eluting of 2mol/L, collect eluent, adjusting pH value is 8, add again 50g sodium hydroxide concentrating under reduced pressure deammoniation water, be concentrated into the amount of 50g, then with the hydrochloric acid of 6mol/L, adjust ph to 6.6, with 20 ℃ of ﹣, preserve 24h, obtain freezing liquid;
E. d step gained freezing liquid is thawed naturally, then with the speed centrifugalize of 3000r/min, remove tyrosine and related impurities, collect supernatant, supernatant is carried out to ultrafiltration with 10000 Dalton molecular weight filter membranes, collect filtrate, then be drying to obtain natural compound amino-acid raw material with 60 ℃ of vacuum drying ovens of ﹣.
Above-mentionedly exemplify 2 typical embodiment, but be not limited to these embodiment.Applicant obtains method of the present invention through repetition test, and has carried out test repeatedly according to the method, can obtain natural compound amino-acid raw material of the present invention.Gained natural compound amino-acid raw material is extracted and is obtained by pig brain tissue, contains 15 seed amino acids.Through calibrating, character: for light yellow to brown color liquid; Differentiate: need testing solution chromatogram and reference substance solution chromatogram are basically identical; PH value is 6.5-7.5.
Claims (7)
1. a preparation method for natural compound amino-acid raw material, is characterized in that comprising the steps:
A. get frozen fresh Medulla sus domestica, thaw, clean, homogenate, adds serosity that concentrated hydrochloric acid stirs evenly, then centrifugalize, removes upper strata emulsion, and collecting precipitation thing, must precipitate brain albumen;
B. add concentrated sulphuric acid to be hydrolyzed the protein precipitation obtaining through above-mentioned steps, filter, obtain acid hydrolysis liquid;
C. by the acid hydrolysis liquid heating obtaining through above-mentioned steps, add active carbon, separation, collects supernatant, and adjust pH is 2.5-4.5, obtains crude extract;
D. by cation exchange column on the crude extract obtaining through above-mentioned steps, adjust pH is 6.5-7.5, uses ammonia eluting, collects eluent, concentrated deammoniation water, and adjust pH is 6.5-7.5,20 ℃ of preservations of ﹣, obtain freezing liquid;
E. will naturally thaw through above-mentioned steps gained freezing liquid, centrifugalize, removes tyrosine and related impurities, collects supernatant, by supernatant ultrafiltration, collects filtrate, dry, obtains required natural compound amino-acid raw material.
2. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterized in that: described a step, get frozen fresh Medulla sus domestica, soaking nature thaws, water cleans again, then the colloid mill homogenate 15min for purified water that adds the heavy 2-4 times of weight of Medulla sus domestica, homogenate gained serosity is poured into after being heated to 70-100 ℃ in extraction pot and is incubated 10-30min, in the heavy every 1kg of Medulla sus domestica, adding the ratio of 2-5 ml to add concentration is again that the concentrated hydrochloric acid of 12mol/L stirs evenly, be cooled to 10-50 ℃, again with the speed centrifugalize of 3000-4000r/min, then remove upper strata emulsion, collecting precipitation thing, must precipitate brain albumen.
3. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterized in that: described b step, by adding the ratio of 750-1500ml to add concentration through a step gained protein precipitation in every 1kg, be the sulphuric acid of 18.4mol/L, make the sulfuric acid solution that concentration is 6-12mol/L, be heated to 110-121 ℃, hydrolysis 6-9h, again with weight ratio 5-10 water dilution doubly, with aqua calcis adjust pH, be 7.5-8.5, then use 200-300 order filter-cloth filtering, remove calcium sulfate precipitation thing, obtain acid hydrolysis liquid.
4. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterized in that: described c step, to be heated to 75-80 ℃ through b step gained acid hydrolysis liquid, the active carbon that adds again acid hydrolysis liquid weight 0.5-1%, be heated to 80-90 ℃, insulation 15min, while hot with the speed centrifugalize 5min of 3000-4000r/min, collecting supernatant, is 2.5-4.5 with oxalic acid solution adjust pH, standing 1-3h, get supernatant, precipitate with the speed centrifugalize 5min of 3000-4000r/min, is removed calcium oxalate precipitation thing again, merges supernatant and obtains crude extract.
5. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterized in that: described d step, to cross cation exchange column through c step gained crude extract, then with purified water rinse to pH value be 6.5-7.5, remove residue oxalic acid and calcium ion, use again the ammonia eluting of 1.8-2mol/L, collect eluent, tune pH value is 8-12, with per kilogram Medulla sus domestica, add the ratio of 1-3g to add sodium hydroxide concentrating under reduced pressure deammoniation water again, be concentrated into the amount of the heavy 1/5-1/3 of Medulla sus domestica, then with the hydrochloric acid of 6mol/L, adjust ph to 6.6-7.5, with 20 ℃ of ﹣, preserve 24h, obtain freezing liquid.
6. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterized in that: described e step, d step gained freezing liquid is thawed naturally, then with the speed centrifugalize of 3000-4000r/min, remove tyrosine and related impurities, collect supernatant, supernatant is carried out to ultrafiltration with 10000 Dalton molecular weight filter membranes, collect filtrate, then be drying to obtain natural compound amino-acid raw material with 60 ℃ of vacuum drying ovens of ﹣ or spraying.
7. a natural compound amino-acid raw material, is characterized in that: described natural compound amino-acid raw material is extracted and obtained by pig brain tissue.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108096233A (en) * | 2018-01-04 | 2018-06-01 | 广东药科大学 | The application of succinic acid, lipoic acid in resisting stress visceral injury drug is prepared |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS606178A (en) * | 1983-06-21 | 1985-01-12 | Yasutsugu Uechi | Processed animal brain |
| CN101912598A (en) * | 2010-06-02 | 2010-12-15 | 刘燎原 | Method for preparing brain protein hydrolysate for injection and preparation thereof |
-
2014
- 2014-07-16 CN CN201410335822.5A patent/CN104127439A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS606178A (en) * | 1983-06-21 | 1985-01-12 | Yasutsugu Uechi | Processed animal brain |
| CN101912598A (en) * | 2010-06-02 | 2010-12-15 | 刘燎原 | Method for preparing brain protein hydrolysate for injection and preparation thereof |
Non-Patent Citations (2)
| Title |
|---|
| 谢瑞娟等: "丝素肽的制备", 《丝绸》 * |
| 陈合等: "《新型食品原料制备技术与应用》", 29 February 2004, 化学工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108096233A (en) * | 2018-01-04 | 2018-06-01 | 广东药科大学 | The application of succinic acid, lipoic acid in resisting stress visceral injury drug is prepared |
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