CN104189007B - The preparation method of natural compound amino-acid raw material - Google Patents
The preparation method of natural compound amino-acid raw material Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of natural compound amino-acid raw material, comprise the following steps：A. take and freeze fresh pig brain, thaw, clean, be homogenized, precipitation brain albumen is collected in separation；B. by protein precipitation drying and crushing, desiccation protein powder is obtained；C. desiccation protein powder is hydrolyzed, diluted, it is 79 to adjust pH value, filtering obtains acid hydrolysis liquid；D. acid hydrolysis liquid is added into activated carbon, separated, collect supernatant, it is 2.5 4.5 to adjust pH value, and precipitation is separated again, merged supernatant and obtain crude extract；E. by cation exchange column on crude extract, it is 6.5 7.5 to be rinsed with water to pH value, and ammoniacal liquor elution collects eluent, is hydrogenated with sodium oxide molybdena, adjusts pH value to 6.6 7.5, ﹣, 20 DEG C of preservations, obtains freezing liquid；F. by freezing liquid naturally to thaw, separation collects supernatant, then ultrafiltration, collects filtered fluid, be drying to obtain natural compound amino-acid raw material.
Description
Technical field
The present invention relates to a kind of preparation method of natural compound amino-acid raw material.
Background technology
Amino acid is the base unit for constituting protein, assigns the specific molecular morphosis of protein, makes his molecule
With biochemical activity.Protein is bioactive molecule important in organism, includes the ferment and enzyme of catalysis metabolism.Amino acid
It is to constitute biological body protein and the most basic material relevant with vital movement, is to constitute protein molecule in vivo
Base unit, has close relationship with biological vital movement.It has special physiological function in antibody, is organism
One of interior indispensable nutritional ingredient.Amino acid can play some following effects in human body by metabolism：1. synthesis group
Textured protein；2. acid, hormone, antibody, creatine etc. are become containing ammoniacal substance；3. it is changed into carbohydrate and fat；4. it is oxidized to
Carbon dioxide and water and urea, produce energy.Therefore, presence of the amino acid in human body, provide not only synthetic protein
Important source material, and for promoting to grow, carry out eubolism, sustain life there is provided material base.
Amino acid can substantially be divided into three classes：1st, essential amino acid：Referring to human body or other vertebrates can not synthesize or close
The need for being far not suitable with body into speed, it is necessary to supplied by food proteins, these amino acid are referred to as essential amino acid.Adult is required
Amount is about the 20-37% of protein requirement the need for amino acid.8 kinds are had, is respectively：Lysine, promotes brain development, is
Liver and the constituent of courage, can promote fat metabolism, regulation pineal body, mammary gland, corpus luteum and ovary prevent cell degradation；Color ammonia
Acid：Promote the generation of gastric juice and pancreatic juice；Phenylalanine：Participate in eliminating the loss of kidney and bladder function；Methionine（Methionine）：
Composition hemoglobin, tissue and serum are participated in, there is the function of promoting spleen, pancreas and lymph；Threonine：Change some amino
Acid reaches the function of balance；Isoleucine：Participate in the regulation and metabolism of thymus gland, spleen and infracerebral gland；Infracerebral gland belongs to commander-in-chief
Portion's effect Thyreoidine, sexual gland；Leucine：Effect balance isoleucine；Valine：Act on corpus luteum, mammary gland and ovary.2nd, half
Essential amino acid and conditionally essential amino acid：Arginine：The compound formulation that arginine is made with deoxycholic acid（Ming Nuofen）It is main
Control the active drug of the disease such as syphilis, viral jaundice；Histidine：Biochemical reagents and medicament can be used as, it may also be used for treatment heart
Disease, anaemia, the medicine of rheumatic arthritis etc..Though human body can synthesize arginine and histidine, it generally can not meet normal
The need for, therefore, be otherwise known as semi-dispensable amino acid or conditionally essential amino acid, and in the physical growth of children phase, both are required amino
Acid.Human body to essential amino acid the need for measure and decline with the increase at age, adult is remarkably decreased than baby.3rd, nonessential ammonia
Base acid：Referring to people or other vertebrates oneself can be synthesized by simple precursor, it is not necessary to the amino acid obtained from food, for example
The amino acid such as glycine, alanine.If human body lacks any essential amino acid, so that it may cause physiological function abnormal, shadow
Being normally carried out for organism metabolism is rung, disease is finally resulted in.Equally, if lacking some nonessential amino acid in human body, it can produce
Organism metabolism obstacle.Arginine and citrulling are particularly significant to forming urea；Cystine insufficiency of intake will cause insulin to subtract
It is few, blood glucose rise.And for example cystine and arginic requirement increase after wound, such as lack, even if heat energy abundance still can not be suitable
Sharp synthetic protein.If human body lacks or reduces wherein a certain, the normal life metabolism of human body will be by obstacle, even
The generation or vital movement for causing various diseases are terminated.So, human body needs to be continuously replenished amino acid, and some diseases need to rely on
Amino acid is treated.
The amino acid products that present people often use are artificial synthesized non-natural Hausmam Amin 20s.This compound
Amino acid transfusion, which has, promotes human body protein Metabolism of Normal, corrects negative nitrogen balance, supplements protein, accelerates the work of wound healing
With.Amino acid transfusion can enter histocyte, participate in the anabolism of protein, obtain in the case where energy supply is sufficient
Positive nitrogen equilibrium, and enzyme, hormone, antibody, structural proteins are generated, promotion organization healing recovers normal physiological function.But it is existing
Compounded amino acid product has very big side effect, and such as Amino Acid Compound Injection can cause rash sample allergic reaction, often has nausea, vomits
Tell, uncomfortable in chest, palpitaition, situations such as feel cold, generate heat or have a headache, using dangerous.
Natural compound amino-acid can overcome the drawbacks described above of artificial synthesized non-natural amino acid, using more pacifying
Entirely.But, existing preparation method total amino acid yield is low, and cost is high.Those skilled in the art catch at a kind of amino acid
The preparation method of the low natural compound amino-acid product of high income, cost, but never solve this technical problem.
The content of the invention
The technical problem to be solved in the present invention is just to provide the low natural compound amino-acid of a kind of amino acid high income, cost
The preparation method of product.
In order to solve the above-mentioned technical problem, the preparation method of natural compound amino-acid raw material of the invention includes following step
Suddenly：
A. take and freeze fresh pig brain, thaw, clean, slurries addition concentrated hydrochloric acid is stirred evenly, is then centrifuged for separation, gone by homogenate
Except upper strata emulsion, sediment is collected, precipitation brain albumen is produced；
B. the protein precipitation obtained through above-mentioned steps dry, pulverize, obtains desiccation protein powder；
C. the desiccation protein powder obtained through above-mentioned steps is added into sulphuric acid hydrolysis, then diluted, tune pH value is 7-9, after
Filter, obtains acid hydrolysis liquid；
D. activated carbon will be added through acid hydrolysis liquid obtained by above-mentioned steps, centrifuged, and collect supernatant, tune pH value is 2.5-4.
5, clear liquid is drawn up, remaining precipitation is centrifuged again, remove calcium oxalate precipitation, merged supernatant and obtain crude extract；
E. will be through cation exchange column on crude extract obtained by above-mentioned steps, it is 6.5-7.5 to be rinsed with water to pH value, is removed
Remaining oxalic acid and calcium ion, are eluted with ammoniacal liquor, collect eluent, are added sodium hydroxide and are concentrated under reduced pressure ammoniacal liquor, then adjust pH value extremely
6.6-7.5, with 20 DEG C of preservations of ﹣, obtains freezing liquid；
F. it will be centrifuged through freezing liquid naturally to thaw obtained by above-mentioned steps, remove tyrosine and related impurities, collected
Supernatant, then milipore filter ultrafiltration is used, filtered fluid is collected, natural compound amino-acid raw material is drying to obtain.
The a steps, take and freeze fresh pig brain, soak naturally to thaw, then are cleaned with water, then add pig brain weight 2-
The purified water of 4 times of weight is homogenized 15min with colloid mill, and homogenate gained slurries are poured into extractor and are heated to protecting after 70-100 DEG C
Warm 10-30min, then the ratio that 2-5 ml are added per 1kg in pig brain weight add concentration and stir evenly, cool down for 12mol/L concentrated hydrochloric acid
5min is separated to 10-50 DEG C, then with 3000-4000r/min centrifugation, upper strata emulsion is then removed, collects sediment,
Produce precipitation brain albumen.
The b step, will be laid on smooth clean flat board rustless steel container through protein precipitation obtained by a steps, smooth
Surface, is allowed to thickness for 1 centimetre, is placed in air dry oven, dries 15-20 hours with airtight heating under the conditions of 78-85 DEG C, so
After crush, obtain desiccation protein powder.
The step c, the ratio of 2000ml sulfuric acid will be added through desiccation protein powder obtained by b step in desiccation protein powder 1kg
Concentration 6mol/L sulfuric acid is added, 8-12h is hydrolyzed under the conditions of 110-121 DEG C, then it is dilute with desiccation protein powder 2-5 times of water of weight
Release, then adjust pH value to be 7.5-8.5 with aqua calcis, then with 200-300 mesh filter-cloth filterings, remove calcium sulfate precipitation, obtain
Acid hydrolysis liquid, the aqua calcis is that the ratio for adding 40-60g calcium hydroxides and 100ml purified waters in 1kg pig brains dissolves
Gained.
The Step d, will be heated to 75-80 DEG C through acid hydrolysis liquid obtained by step c, then add 0.5-1% activated carbons, continue to add
Then heat be incubated 15min to 80-90 DEG C, and 5min is separated with 3000-4000r/min centrifugations, collects supernatant, uses oxalic acid
Solution adjusts pH value to be 2.5-4.5, stands 1-3h, draws up clear liquid, and remaining precipitation separates 5min with 3000-4000r/min centrifugations,
Calcium oxalate precipitation is removed, merges supernatant and obtains crude extract, the oxalic acid solution adds 10-15g oxalic acid and 100ml with every 1kg pig brains
The ratio of purified water, which is dissolved by heating, to be made.
The step e, by cation exchange column on crude extract obtained by Step d, it is 6.5-7.5 to be rinsed with water to pH value, is gone
Eluted except remaining oxalic acid and calcium ion, then with 1.8-2mol/L ammoniacal liquor, collect eluent, it is 8.0-12.0 to make pH value, then by 1kg
The ratio that pig brain adds 1-3g sodium hydroxides adds sodium hydroxide and is concentrated under reduced pressure ammoniacal liquor, is concentrated into pig brain weight 1/5-1/3, then
PH value is adjusted to 6.6-7.5 with 6mol hydrochloric acid, then preserves 24h with 20 DEG C of conditions of ﹣, obtains freezing liquid.
The f steps, by freezing liquid naturally to thaw obtained by step e, under the conditions of 0 DEG C with 3000-4000r/min speed from
The heart is separated, and removes tyrosine and related impurities, collects supernatant, then with 10000 molecular weight milipore filter ultrafiltration, collects filtered fluid,
Then with 60 DEG C of vacuum drying chambers of ﹣ or spray drying, natural compound amino-acid raw material is obtained.
The method of the present invention will precipitation brain albumen be first dried, desiccation protein powder is made in crushing, adds sulphuric acid hydrolysis, can pole
The earth improves the yield of total amino acid.
The method of the present invention is applicant by obtained by development test repeatedly.
In test, operated according to uniform requirement, meet acid hydrolysis conditions, other conditions are identical, only to precipitation brain egg
The dry state of white spirit is different, and last gained total nitrogen and amino acid content result are as follows：
As can be seen here, the drying steps of purifying protein have a significant impact to finished product yield, same process condition and processing
In time, because degree of drying is different or unseasoned, the intermediary outcomes control of whole process is influenceed after hydrolysis, water content is big
When, obtain finished product consuming cost difference less, but the decline of total amino acid yield is extracted, it is not easy to stability contorting；Increase drying
After step, after especially size-reduced obtained desiccation protein powder, then hydrolyze, the yield of total amino acid can be greatly improved.It is thus relative to drop
Low preparation cost.
Natural compound amino-acid raw material prepared by the inventive method can be used in preparing to be produced using the compound amino acid of safety
Product, are used and health of masses food and health products such as food available for medicine such as parenteral solution, freeze-dried powder and oral formulations are prepared
Additive etc..
The method of the present invention is applied to be prepared on a large scale pure natural composite amino-acid（Have 15 kinds of amino acid）Raw material, method
Simple controllable, by disposably handling a large amount of pig brain tissues, applied sample amount is big during purifying, can carry out natural compound amino-acid raw material
Large-scale production, suitable for heavy industrialization application.
Embodiment
The following detailed description of the embodiment of the inventive method, but it is not limited only to following examples.
Embodiment one
The preparation method step of the natural compound amino-acid raw material of the present invention：
A. take and freeze fresh pig brain 50kg, soak naturally to thaw, then cleaned with water, then add 100kg purified waters
15min is homogenized with colloid mill, homogenate gained slurries is poured into extractor after being heated to 75 DEG C and is incubated 15min, add 250
Ml concentration stirs evenly for 12mol/L concentrated hydrochloric acid, is cooled to 10 DEG C, then separates 5min with 3000r/min centrifugation, then goes
Except upper strata emulsion, sediment is collected, precipitation brain protein 23 .3kg is produced；
B. it will be laid in through protein precipitation obtained by a steps on smooth clean flat board rustless steel container, flat surface makes
Thickness be 1 centimetre, be placed in air dry oven, with the conditions of 78 DEG C airtight heating dry 15 hours, then crush, obtain drying
Albumen powder 1.1kg；
C. 2.2kg concentration 6mol/L sulfuric acid will be added through desiccation protein powder obtained by b step, is hydrolyzed under the conditions of 110 DEG C
8h, then diluted with 4.4kg water, then adjust pH value to be 7.5 with aqua calcis, then with 200 mesh filter-cloth filterings, remove calcium sulfate
Precipitation, obtains acid hydrolysis liquid, and the aqua calcis is the ratio that 40g calcium hydroxides and 100ml purified waters are added in 1kg pig brains
Dissolving gained；
D. 75 DEG C will be heated to through acid hydrolysis liquid obtained by step c, then adds 0.5% activated carbon, continues to be heated to 80 DEG C, then
15min is incubated, 5min is separated with 3000r/min centrifugations, supernatant is collected, adjusts pH value to be 3.5 with oxalic acid solution, stands
3h, draws up clear liquid, and remaining precipitation separates 5min with 3000r/min centrifugations, removes calcium oxalate precipitation, and merging supernatant, which is obtained, slightly to be carried
Liquid, it is obtained that the oxalic acid solution adds the ratio of 10g oxalic acid and 100ml purified waters to dissolve by heating with every 1kg pig brains；
E. by cation exchange column on crude extract obtained by Step d, it is 6.5 to be rinsed with water to pH value, remove remaining oxalic acid and
Calcium ion, then eluted with 1.8mol/L ammoniacal liquor, eluent is collected, it is 8 to make pH value, adds 50g sodium hydroxides and is concentrated under reduced pressure
Ammoniacal liquor, is concentrated into 17kg, then adjusts pH value to 6.6 with 6mol hydrochloric acid, then preserves 24h with 20 DEG C of conditions of ﹣, obtains freezing liquid；
F. by freezing liquid naturally to thaw obtained by step e, separated, removed with 3000r/min centrifugations under the conditions of 0 DEG C
Tyrosine and related impurities, collect supernatant, then with 10000 molecular weight milipore filter ultrafiltration, collect filtered fluid, then spray dry
It is dry, obtain natural compound amino-acid raw material.
Embodiment two
A. take and freeze fresh pig brain 50kg, soak naturally to thaw, then cleaned with water, then add 150kg purified waters
15min is homogenized with colloid mill, homogenate gained slurries is poured into extractor after being heated to 85 DEG C and is incubated 15min, add 250
Ml concentration stirs evenly for 12mol/L concentrated hydrochloric acid, is cooled to 30 DEG C, then separates 5min with 3500r/min centrifugation, then goes
Except upper strata emulsion, sediment is collected, precipitation brain protein 23 .1kg is produced；
B. it will be laid in through protein precipitation obtained by a steps on smooth clean flat board rustless steel container, flat surface makes
Thickness be 1 centimetre, be placed in air dry oven, with the conditions of 80 DEG C airtight heating dry 15 hours, then crush, obtain drying
Albumen powder 1.099kg；
C. 2.2kg concentration 6mol/L sulfuric acid will be added through desiccation protein powder obtained by b step, is hydrolyzed under the conditions of 115 DEG C
8h, then diluted with 4.4kg water, then adjust pH value to be 8.5 with aqua calcis, then with 300 mesh filter-cloth filterings, remove calcium sulfate
Precipitation, obtains acid hydrolysis liquid, and the aqua calcis is the ratio that 50g calcium hydroxides and 100ml purified waters are added in 1kg pig brains
Dissolving gained；
D. 78 DEG C will be heated to through acid hydrolysis liquid obtained by step c, then adds 0.8% activated carbon, continues to be heated to 85 DEG C, then
15min is incubated, 5min is separated with 3500r/min centrifugations, supernatant is collected, adjusts pH value to be 3.5 with oxalic acid solution, stands
3h, draws up clear liquid, and remaining precipitation separates 5min with 3500r/min centrifugations, removes calcium oxalate precipitation, and merging supernatant, which is obtained, slightly to be carried
Liquid, it is obtained that the oxalic acid solution adds the ratio of 15g oxalic acid and 100ml purified waters to dissolve by heating with every 1kg pig brains；
E. by cation exchange column on crude extract obtained by Step d, it is 7 to be rinsed with water to pH value, removes remaining oxalic acid and calcium
Ion, then eluted with 1.9mol/L ammoniacal liquor, eluent is collected, it is 10 to make pH value, adds 100g sodium hydroxides and is concentrated under reduced pressure
Ammoniacal liquor, is concentrated into 12.5kg, then adjusts pH value to 7 with 6mol hydrochloric acid, then preserves 24h with 20 DEG C of conditions of ﹣, obtains freezing liquid；
F. by freezing liquid naturally to thaw obtained by step e, separated, removed with 3800r/min centrifugations under the conditions of 0 DEG C
Tyrosine and related impurities, collect supernatant, then with 10000 molecular weight milipore filter ultrafiltration, collect filtered fluid, then spray dry
It is dry, obtain natural compound amino-acid raw material.
Embodiment three
A. take and freeze fresh pig brain 50kg, soak naturally to thaw, then cleaned with water, then add 200kg purified waters
15min is homogenized with colloid mill, homogenate gained slurries is poured into extractor after being heated to 100 DEG C and is incubated 30min, add 250
Ml concentration stirs evenly for 12mol/L concentrated hydrochloric acid, is cooled to 50 DEG C, then separates 5min with 4000r/min centrifugation, then goes
Except upper strata emulsion, sediment is collected, precipitation brain 4 protein 22 .9kg is produced；
B. it will be laid in through protein precipitation obtained by a steps on smooth clean flat board rustless steel container, flat surface makes
Thickness be 1 centimetre, be placed in air dry oven, with the conditions of 85 DEG C airtight heating dry 20 hours, then crush, obtain drying
Albumen powder 1.098kg；
C. 2.2kg concentration 6mol/L sulfuric acid will be added through desiccation protein powder obtained by b step, is hydrolyzed under the conditions of 121 DEG C
12h, then diluted with 4.4kg water, then adjust pH value to be 8.5 with aqua calcis, then with 300 mesh filter-cloth filterings, go sulfuric acid
Calcium precipitate, obtains acid hydrolysis liquid, and the aqua calcis is to add 50g calcium hydroxides and the ratio of 100ml purified waters by 1kg pig brains
Example dissolving gained；
D. 80 DEG C will be heated to through acid hydrolysis liquid obtained by step c, then adds 1% activated carbon, continues to be heated to 90 DEG C, Ran Houbao
Warm 15min, 5min is separated with 4000r/min centrifugations, collects supernatant, is adjusted pH value to be 4.5 with oxalic acid solution, is stood 3h,
Clear liquid is drawn up, remaining precipitation separates 5min with 4000r/min centrifugations, removes calcium oxalate precipitation, merges supernatant and obtain crude extract,
It is obtained that the oxalic acid solution adds the ratio of 15g oxalic acid and 100ml purified waters to dissolve by heating with every 1kg pig brains；
E. by cation exchange column on crude extract obtained by Step d, it is 7.5 to be rinsed with water to pH value, remove remaining oxalic acid and
Calcium ion, then eluted with 2mol/L ammoniacal liquor, eluent is collected, it is 12 to make pH value, adds 150g sodium hydroxides and is concentrated under reduced pressure
Ammoniacal liquor, is concentrated into 10kg, then adjusts pH value to 7.5 with 6mol hydrochloric acid, then preserves 24h with 20 DEG C of conditions of ﹣, obtains freezing liquid；
F. by freezing liquid naturally to thaw obtained by step e, separated, removed with 4000r/min centrifugations under the conditions of 0 DEG C
Tyrosine and related impurities, collect supernatant, then with 10000 molecular weight milipore filter ultrafiltration, collect filtered fluid, then with 60 DEG C of ﹣
Vacuum drying chamber is dried, and obtains natural compound amino-acid raw material.
Natural compound amino-acid raw material obtained by the above method is fixed molten, by as allotment is former after stoste standard test content
Liquid.Through examining and determine above-mentioned stoste, quality standard is complied fully with：(1) character is light yellow to brown color liquid；(2) reflect
Not, need testing solution chromatogram and reference substance solution chromatogram are basically identical；(3) pH values, 6.5-7.5；(4) naturally it is combined ammonia
Base acid content meets standard regulation, and during total nitrogen 5.49-6.71mg/ml, 15 kinds of compound amino acids are respectively L-aminobutanedioic acid 3.84-
5.76th, serine 1.82-3.38, glutamic acid 4.96-7.44, glycine 1.92-2.88, histidine 0.72-1.08, arginine
0.30-1.90, threonine 0.84-1.86, alanine 2.00-3.00, proline 1.6-2.5, valine 1.28-1.92, first sulphur
Propylhomoserin 2.96-4.44, lysine 2.96-4.44, isoleucine 0.88-1.32, leucine 2.24-3.36, phenylalanine 0.96-
1.44,15 kinds of natural compound amino-acid total amounts are 28.08-43.62mg/ml.
Claims (5)
1. a kind of preparation method of natural compound amino-acid raw material, it is characterised in that comprise the following steps：
A. take and freeze fresh pig brain, thaw, clean, slurries addition concentrated hydrochloric acid is stirred evenly, is then centrifuged in separation, removal by homogenate
Layer emulsion, collects sediment, produces precipitation brain albumen；
B. the precipitation brain albumen obtained through above-mentioned steps dry, pulverize, obtains desiccation protein powder, will be through being precipitated obtained by a steps
Albumen is laid on smooth clean flat board rustless steel container, flat surface, is allowed to thickness for 1 centimetre, is placed in air dry oven
It is interior, dried 15-20 hours with airtight heating under the conditions of 78-85 DEG C, then crush, obtain desiccation protein powder；
C. the desiccation protein powder obtained through above-mentioned steps is added into sulphuric acid hydrolysis, then diluted, tune pH value is 7-9, is refiltered,
Obtain acid hydrolysis liquid；
D. activated carbon will be added through acid hydrolysis liquid obtained by above-mentioned steps, centrifuged, and collect supernatant, tune pH value is 2.5-4. 5,
Clear liquid is drawn up, remaining precipitation is centrifuged again, remove calcium oxalate precipitation, merged supernatant and obtain crude extract；
E. will be through cation exchange column on crude extract obtained by above-mentioned steps, it is 6.5-7.5 to be rinsed with water to pH value, is removed remaining
Oxalic acid and calcium ion, are eluted with ammoniacal liquor, collect eluent, are added sodium hydroxide and are concentrated under reduced pressure ammoniacal liquor, then adjust pH value to 6.6-
7.5, with 20 DEG C of preservations of ﹣, obtain freezing liquid；
F. it will be centrifuged through freezing liquid naturally to thaw obtained by above-mentioned steps, remove tyrosine and related impurities, collect supernatant
Liquid, then milipore filter ultrafiltration is used, filtered fluid is collected, natural compound amino-acid raw material is drying to obtain.
2. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterised in that：The a steps, take jelly
Fresh pig brain is deposited, naturally to thaw is soaked, then is cleaned with water, the purified water colloid of pig brain 2-4 times of weight of weight is then added
Mill homogenate 15min, homogenate gained slurries are poured into extractor and are heated to after 70-100 DEG C being incubated 10-30min, then by pig brain weight
The ratio addition concentration that 2-5 ml are added per 1kg stirs evenly for 12mol/L concentrated hydrochloric acid, is cooled to 10-50 DEG C, then with 3000-
4000r/min centrifugation separation 5min, then removes upper strata emulsion, collects sediment, produce precipitation brain albumen.
3. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterised in that：The step c, will be through
Desiccation protein powder obtained by b step is added concentration 6mol/L sulfuric acid in the desiccation protein powder 1kg ratios for adding 2000ml sulfuric acid,
8-12h is hydrolyzed under the conditions of 110-121 DEG C, then is diluted with 2-5 times of water of desiccation protein powder weight, then pH is adjusted with aqua calcis
It is worth for 7.5-8.5, then with 200-300 mesh filter-cloth filterings, removes calcium sulfate precipitation, obtain acid hydrolysis liquid, the aqua calcis
To add 40-60g calcium hydroxides and the ratio dissolving gained of 100ml purified waters in 1kg pig brains.
4. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterised in that：The Step d, will be through
Acid hydrolysis liquid obtained by step c is heated to 75-80 DEG C, then adds 0.5-1% activated carbons, continues to be heated to 80-90 DEG C, is then incubated
15min, 5min is separated with 3000-4000r/min centrifugations, collects supernatant, adjusts pH value to be 2.5-4.5 with oxalic acid solution,
1-3h is stood, clear liquid is drawn up, remaining precipitation separates 5min with 3000-4000r/min centrifugations, removes calcium oxalate precipitation, merges
Supernatant obtains crude extract, and the oxalic acid solution adds the ratio of 10-15g oxalic acid and 100ml purified waters to dissolve by heating with every 1kg pig brains
It is made.
5. the preparation method of natural compound amino-acid raw material according to claim 1, it is characterised in that：The step e, by d
Cation exchange column on crude extract obtained by step, it is 6.5-7.5 to be rinsed with water to pH value, removes remaining oxalic acid and calcium ion, then
Eluted with 1.8-2mol/L ammoniacal liquor, collect eluent, it is 8.0-12.0 to make pH value, then adds 1-3g sodium hydroxides by 1kg pig brains
Ratio add sodium hydroxide and be concentrated under reduced pressure ammoniacal liquor, be concentrated into pig brain weight 1/5-1/3, then adjust pH value extremely with 6mol hydrochloric acid
6.6-7.5, then 24h is preserved with 20 DEG C of conditions of ﹣, obtain freezing liquid.
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| 脑蛋白水解工艺的研究;吕晓明;《科技创新导报》;20091231;第6-7页 * |
| 脑蛋白水解新工艺的研究;白云起等;《化工时刊》;20031231;第17卷(第12期);第30-33页，尤其是第30页左栏倒数第1行至右栏第1段第1-4行，第31页3.4.1脑蛋白粉的制备项下，第32页3.4.5微波对水解的影响项下 * |
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