CN104108962A - Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast - Google Patents
Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast Download PDFInfo
- Publication number
- CN104108962A CN104108962A CN201410313901.6A CN201410313901A CN104108962A CN 104108962 A CN104108962 A CN 104108962A CN 201410313901 A CN201410313901 A CN 201410313901A CN 104108962 A CN104108962 A CN 104108962A
- Authority
- CN
- China
- Prior art keywords
- selenium
- yeast rich
- fertilizer
- rich
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fertilizers (AREA)
Abstract
The invention relates to a method for preparing an organic selenium fertilizer through hydrolysis of selenium-enriched yeast. The method comprises the following steps: with selenium-enriched yeast as a raw material, allowing selenium-enriched yeast cells to undergone wall breaking and selenoprotein to undergone hydrolysis under acidic and thermal conditions so as to obtain selenoamino acid and oligopeptides thereof; carrying out cooling to room temperature; and adjusting the pH value of hydrolysate to allow the hydrolysate to be neutral, thereby obtaining the micromolecular organic selenium fertilizer. The method has the advantages of easiness, convenient operation and easy realization of industrial production. The prepared organic selenium fertilizer has the main components--selenoamino acid and oligopeptides thereof, has a small molecular weight and can be easily absorbed by plants. When the micromolecular organic selenium fertilizer is applied to crops, the contents of organic selenium in fruit and seeds are greatly increased, the outputs of the crops are increased, the added values of agricultural products are enhanced, and economic benefits of the crops are improved.
Description
Technical field
The present invention relates to a kind of preparation method of organic selenium fertilizer, be specifically related to a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed.
Background technology
Selenium is the essential trace element of humans and animals, and scarce selenium can have a strong impact on the healthy of people.1972, Roetueh confirmed that selenium is the activeconstituents of Selenoperoxidase (GPX), and from then on, selenium is acknowledged as the necessary trace element of life.
Selenium is the essential nutritive element of higher plant growth, and according to the study, 0.1～1.0 μ g/g can promote growth, the increase output of paddy rice and increase the selenium content in seed.The selenium of 1.0 μ g/g can obviously improve the activity of the Selenoperoxidase (GPX) in the improving activity of root system of rice seedling and tillering phase, boot stage, and the selenium content in seed is apparently higher than control group.
Inorganic selenium enters to be got rid of after human body externally very soon by excretion pathway, human absorptivity is very low, so its toxicity is greater than organoselenium, and activity is lower than organoselenium, is conventionally not suitable for directly adding in food.Human body is mended selenium and need to from the foods such as rice, wheat-flour, vegetables, fruit, be absorbed.Organic selenium fertilizer is the effective means that improves the Se contents such as grain, fruit, vegetables.
Yeast has the selenium rich ability of height, inorganic selenium can be changed into organoselenium, and organoselenium wherein exists mainly with the form of seleno-protein and selenium polysaccharide greatly, because its molecular weight is large, is not easy to be absorbed fast by plant.
It is raw material that application number 01113654.5 be take plant cake or plant protein, is hydrolyzed, then adds selenide and make containing selenoamino acid under high mild acidic conditions.Essence is the mixture of amino acid and inorganic selenium, non-organoselenium, and effect and the security of use are under suspicion.
Application number 201110440135.6 by yeast rich in selenium under alkaline condition with after supersonic wave wall breaking, extract seleno-protein for foodstuff additive.Do not belong to a field with the present invention, the molecular weight of product is also much bigger than the present invention.Because its molecular weight can not be absorbed fast by plant greatly, inconvenience is done organic selenium fertilizer quick-acting fertilizer and is used, and its complex process, and manufacturing cost is higher.
Application number CN102219586 A makes organic selenium fertilizer by fertilizer and Sodium Selenite mixing, has a large amount of inorganic seleniums in its product, cannot well be absorbed by plant.
In view of this, need to invent a kind of preparation method who is conducive to improve the organic selenium fertilizer of farm crop organic selenium content.
Summary of the invention
The object of the invention is to overcome the defect of prior art, a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed is provided, the method step is simple, and process stabilizing can obtain a kind of as the widely used small molecules organic selenium fertilizer of farm crop.
For achieving the above object, the technical solution used in the present invention is:
Be hydrolyzed yeast rich in selenium and prepare a method for organic selenium fertilizer, it specifically comprises the steps:
(1) prepare yeast rich in selenium liquid
First by following either method, prepare yeast rich in selenium liquid:
A mixes yeast rich in selenium and water, is prepared into the mass volume ratio containing yeast rich in selenium 3%～20%() bacteria suspension; Or
B is used yeast rich in selenium fermenation raw liquid;
(2) hydrolysis broken wall
The yeast rich in selenium liquid pH value that step (1) is obtained is adjusted to acidity with acid, and heating is hydrolyzed into containing the acid solution that is rich in seleno-amino acids and oligopeptides thereof;
(3) preparation of organic selenium fertilizer
By resulting adjusting PH with base to 6.0～8.0 for acid solution of step (2), both the small molecules organic selenium fertilizer of seleno-amino acids and oligopeptides thereof.
Acid in above-mentioned steps (2) is selected from hydrochloric acid or sulfuric acid.
The acidity of above-mentioned steps (2) refers to that pH is 0.5～4.0.
In above-mentioned steps (2), the temperature of heating is 105～160 ℃.
Alkali in above-mentioned steps (3) is selected from potassium hydroxide, ammoniacal liquor or calcium hydroxide.
Preferably, the acid in above-mentioned steps (2) is hydrochloric acid.
Preferably, the acidity in above-mentioned steps (2) refers to that pH is 1.0～3.0.
Preferably, in above-mentioned steps (2), the temperature of heating is 140～160 ℃.
Preferably, the alkali in above-mentioned steps (3) is selected from calcium hydroxide.
Preferably, the pH in above-mentioned steps (3) is 6.5～7.2.
Preferably, the selenium in selenium enriched yeast in above-mentioned steps (1) is 500～2000ppm.
Preferably, the selenium in selenium enriched yeast in above-mentioned steps (1) is 1000～2000ppm.
As a kind of the solution of the present invention, be a kind ofly hydrolyzed the method that yeast rich in selenium is prepared organic solarization fertilizer, it specifically comprises the steps:
(1) prepare yeast rich in selenium liquid
First by following either method, prepare yeast rich in selenium liquid:
A mixes yeast rich in selenium and water, is prepared into containing yeast rich in selenium 3%～20% bacteria suspension; Or
B is used yeast rich in selenium fermenation raw liquid;
(2) hydrolysis broken wall
The yeast rich in selenium liquid that step (1) is obtained, with hydrochloric acid adjust pH to 0.5～4.0, is heated to 105～160 ℃, is incubated 0.5～8 hour, is hydrolyzed into containing the acid solution that is rich in seleno-amino acids and oligopeptides thereof;
(3) preparation of organic selenium fertilizer
The resulting acid solution of step (2) is adjusted to pH to 6.0～8.0 with calcium hydroxide, both obtain the small molecules organic selenium fertilizer of seleno-amino acids and oligopeptides thereof.
As preferred scheme, be a kind ofly hydrolyzed the method that yeast rich in selenium is prepared organic selenium fertilizer, it specifically comprises the steps:
(1) prepare yeast rich in selenium liquid
First by following either method, prepare yeast rich in selenium liquid:
A mixes yeast rich in selenium and water, is prepared into containing yeast rich in selenium 3%～20% bacteria suspension; Or
B is used yeast rich in selenium fermenation raw liquid;
(2) hydrolysis broken wall
The yeast rich in selenium liquid that step (1) is obtained, with hydrochloric acid adjust pH to 1～3, is heated to 140～160 ℃, and insulation 0.5-8 hour is hydrolyzed into containing the acid solution that is rich in seleno-amino acids and oligopeptides thereof;
(3) preparation of organic selenium fertilizer
The resulting acid solution of step (2) is adjusted to pH to 6.5～7.2 with calcium hydroxide, both obtain the small molecules organic selenium fertilizer of seleno-amino acids and oligopeptides thereof.
Described yeast rich in selenium fermenation raw liquid is prepared by the following method: yeast is seeded in the sterilized liquid nutrient medium containing inorganic selenium, at a certain temperature, makes the nutrient solution of gained after yeast amount reproduction.
Acid in step (2) is concentrated acid, if its concentration >=25% of hydrochloric acid.Its concentration >=70% of sulfuric acid if.
Compared with prior art, the beneficial effect that the present invention obtains is:
(1) the present invention is by the optimization to hydrolysising condition, make the sporoderm-broken rate of yeast rich in selenium high, seleno proteolysis becomes the molecular weight of selenium-rich amino acid and oligopeptides thereof little, a kind of widely used small molecules organic selenium fertilizer of farm crop that can be used as is provided, comparing the fertile toxicity of inorganic selenium reduces greatly, being convenient to plant absorbs fast, for producing rich selenium grain, the high yield of rich selenium fruit, rich selenium vegetables and farm crop provides better selection.
(2) by method of the present invention, produce small molecules organic selenium fertilizer, production technique is simple, and facility investment is little, low in the pollution of the environment, is easy to industry and promotes.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in more detail, the molecular weight of the resulting organic selenium fertilizer of following embodiment is after testing between 168 ~ 500.
Embodiment 1
Taking 5 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 50ml purified water, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 2.0, put into autoclaving still, with being steam heated to 140 ℃, be incubated 6 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Embodiment 2
Taking 5 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 50ml purified water, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 3.0, put into autoclaving still, with being steam heated to 140 ℃, be incubated 6 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Embodiment 3
Taking 5 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 50ml purified water, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 4.0, put into autoclaving still, with being steam heated to 140 ℃, be incubated 6 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Embodiment 4
Taking 5 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 50ml purified water, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 3.0, put into autoclaving still, with being steam heated to 150 ℃, be incubated 5 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Embodiment 5
Taking 5 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 50ml purified water, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 3.0, put into autoclaving still, with being steam heated to 160 ℃, be incubated 4 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Embodiment 6
Taking 6 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 200ml purified water, fully shake up, make bacteria suspension, with 70% concentrated hydrochloric acid, regulating pH is 0.5, put into autoclaving still, with being steam heated to 105 ℃, be incubated 8 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% potassium hydroxide aqueous solution, regulate PH to 8.0, obtain small molecules organic selenium fertilizer.
Embodiment 7
Taking 6 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 200ml purified water, fully shake up, make bacteria suspension, with 70% vitriol oil, regulating pH is 4.0, put into autoclaving still, with being steam heated to 160 ℃, be incubated 3 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 8.0, obtain small molecules organic selenium fertilizer.
Embodiment 8
Taking 5 grams of selenium contents is that the rich selenium dry yeast of 2000ppm is put in the 500ml triangular flask that adds in advance 50ml purified water, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 1.0, put into autoclaving still, with being steam heated to 150 ℃, be incubated 4 hours, yeast rich in selenium is hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Embodiment 9
Yeast is seeded in the sterilized liquid nutrient medium containing inorganic selenium, at a certain temperature, makes to obtain yeast rich in selenium stoste after yeast amount reproduction.Get 50ml and join in 500ml triangular flask, fully shake up, make bacteria suspension, with 30% concentrated hydrochloric acid, regulating pH is 1.0, puts into autoclaving still, with being steam heated to 150 ℃, be incubated 4 hours, yeast rich in selenium be hydrolyzed into the small molecules organoselenium of seleno-amino acids and oligopeptides thereof; When temperature is down to after room temperature, with 10% calcium hydroxide aqueous solution, regulate PH to 6.5-7.2, obtain small molecules organic selenium fertilizer.
Effect example 1
Utilize the organic selenium fertilizer that the embodiment of the present invention 1 prepares to carry out wheatland test.
Material:
Test fertilizer is the resulting organic selenium fertilizer of the present embodiment 1
30 mu of wheatland areas, the sowing control measures such as apply fertilizer to the subsoil, water are consistent, and wheat booting period in 2014 and filling stage are divided into the Er Ge district controlled trial of applying fertilizer:
Test site: test fertile 2 Kg/ mus; Dilute 100 times, all foliage-spray at twice
Clear area: do not apply fertilizer
Result shows: use the fertile wheat of test and the wheat comparison of not applying fertilizer, leaf is thick and large, and wheat fruit fringe is praised greatly, quality better, and each index is in Table 1:
Table 1
| Project | Selenium content (μ g/Kg) | Stimulation ratio |
| Test site | 207 | 22.4% |
| Clear area | 23 | —— |
Conclusion: small molecules organic selenium fertilizer provided by the present invention, can significantly promote the absorption of farm crop to selenium, improve farm crop organic selenium content, meet the demand of people to selenium content in grain.
The above embodiment is only the preferred embodiments of the present invention, and is not the exhaustive of the feasible enforcement of the present invention.For persons skilled in the art, any apparent change of under the prerequisite that does not deviate from the principle of the invention and spirit, it having been done, within all should being contemplated as falling with claim protection domain of the present invention.
Claims (10)
1. be hydrolyzed yeast rich in selenium and prepare a method for organic selenium fertilizer, it is characterized in that: it specifically comprises the steps:
(1) prepare yeast rich in selenium liquid
First by following either method, prepare yeast rich in selenium liquid:
A mixes yeast rich in selenium and water, is prepared into containing yeast rich in selenium 3%～20% bacteria suspension; Or
B is used yeast rich in selenium fermenation raw liquid;
(2) hydrolysis broken wall
The yeast rich in selenium liquid pH value that step (1) is obtained is adjusted to acidity with acid, and heating is hydrolyzed into containing the acid solution that is rich in seleno-amino acids and oligopeptides thereof;
(3) preparation of organic selenium fertilizer
By resulting adjusting PH with base to 6.0～8.0 for acid solution of step (2), both the small molecules organic selenium fertilizer of seleno-amino acids and oligopeptides thereof.
2. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: the acid in described rapid (2) is selected from hydrochloric acid or sulfuric acid; Hydrochloric acid preferably.
3. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: the acidity of described step (2) refers to that pH is 0.5～4.0; Preferably, the acidity of described step (2) refers to that pH is 1.0～3.0.
4. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: in described step (2), the temperature of heating is 105～160 ℃; Preferably, in described step (2), the temperature of heating is 140～160 ℃.
5. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: the alkali in described step (3) is selected from potassium hydroxide, ammoniacal liquor or calcium hydroxide; Preferably, the alkali in described step (3) is calcium hydroxide.
6. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: the pH in described step (3) is 6.5～7.2.
7. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: the selenium in selenium enriched yeast in described step (1) is 500～2000ppm.
8. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 1, is characterized in that: the selenium in selenium enriched yeast in described step (1) is 1000～2000ppm.
9. according to a kind of described in claim 1-8 any one, be hydrolyzed the method that yeast rich in selenium is prepared organic selenium fertilizer, it is characterized in that: it specifically comprises the steps:
(1) prepare yeast rich in selenium liquid
First by following either method, prepare yeast rich in selenium liquid:
A mixes yeast rich in selenium and water, is prepared into containing yeast rich in selenium 3%～20% bacteria suspension; Or
B is used yeast rich in selenium fermenation raw liquid;
(2) hydrolysis broken wall
The yeast rich in selenium liquid that step (1) is obtained, with hydrochloric acid adjust pH to 0.5～4.0, is heated to 105～160 ℃, and insulation 0.5-8 hour is hydrolyzed into containing the acid solution that is rich in seleno-amino acids and oligopeptides thereof;
(3) preparation of organic selenium fertilizer
The resulting acid solution of step (2) is adjusted to pH to 6.0～8.0 with calcium hydroxide, both obtain the small molecules organic selenium fertilizer of seleno-amino acids and oligopeptides thereof.
10. a kind of method that yeast rich in selenium is prepared organic selenium fertilizer that is hydrolyzed according to claim 9, is characterized in that: it specifically comprises the steps:
(1) prepare yeast rich in selenium liquid
First by following either method, prepare yeast rich in selenium liquid:
A mixes yeast rich in selenium and water, is prepared into containing yeast rich in selenium 3%～20% bacteria suspension; Or
B is used yeast rich in selenium fermenation raw liquid;
(2) hydrolysis broken wall
The yeast rich in selenium liquid that step (1) is obtained, with hydrochloric acid adjust pH to 1～3, is heated to 140～160 ℃, and insulation 0.5-8 hour is hydrolyzed into containing the acid solution that is rich in seleno-amino acids and oligopeptides thereof;
(3) preparation of organic selenium fertilizer
The resulting acid solution of step (2) is adjusted to pH to 6.5～7.2 with calcium hydroxide, both obtain the small molecules organic selenium fertilizer of seleno-amino acids and oligopeptides thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410313901.6A CN104108962A (en) | 2014-07-03 | 2014-07-03 | Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410313901.6A CN104108962A (en) | 2014-07-03 | 2014-07-03 | Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104108962A true CN104108962A (en) | 2014-10-22 |
Family
ID=51705975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410313901.6A Pending CN104108962A (en) | 2014-07-03 | 2014-07-03 | Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104108962A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521550A (en) * | 2014-12-01 | 2015-04-22 | 云南金雨庄生物科技有限责任公司 | Selenium-rich grifola frondosa cultivation method |
| CN105198676A (en) * | 2015-11-09 | 2015-12-30 | 中南林业科技大学 | Selenium-rich biological organic fertilizer for camellia oleifera as well as preparation method and application of selenium-rich biological organic fertilizer |
| CN105272412A (en) * | 2015-10-23 | 2016-01-27 | 广西锦翰环保科技有限公司 | Organic-Se nutrient solution for crops as well as preparation method and application of organic-Se nutrient solution |
| CN107586190A (en) * | 2017-08-03 | 2018-01-16 | 浙江省农业科学院 | A kind of tea tree charcoal base selenium-enriched fertilizer |
| CN105130531B (en) * | 2015-07-31 | 2018-11-20 | 中国农业科学院农产品加工研究所 | A kind of compound organism organic selenium fertilizer and its preparation method and application |
| CN109336687A (en) * | 2018-11-19 | 2019-02-15 | 黑龙江八农垦大学 | Hydrolyzed yeast zinc foliar fertilizer, production method and application |
| CN109485490A (en) * | 2018-11-19 | 2019-03-19 | 黑龙江八农垦大学 | Broken wall yeast zinc foliar fertilizer, production method and application |
| CN109534893A (en) * | 2018-11-19 | 2019-03-29 | 黑龙江八农垦大学 | Hydrolyzed yeast selenium foliar fertilizer, production method and application |
| CN112079658A (en) * | 2020-09-02 | 2020-12-15 | 王雪薇 | Preparation and application of plant nutrient solution rich in glucose tolerance factor |
| CN112851412A (en) * | 2021-03-01 | 2021-05-28 | 上海交通大学 | Novel organic selenium fertilizer and preparation method thereof |
| CN113383688A (en) * | 2021-06-16 | 2021-09-14 | 黑龙江植今农业科技有限责任公司 | Production method of selenium-rich rice |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008015334A2 (en) * | 2006-07-28 | 2008-02-07 | Ithec (Innovation Technique Expansion Commerciale) | Method for treating crop plants to enrich same with selenium and use of plants so treated |
| CN102550795A (en) * | 2011-12-26 | 2012-07-11 | 苏州硒谷科技有限公司 | Method for extracting selenium-containing protein from selenium-enriched yeast |
| CN103626587A (en) * | 2013-12-03 | 2014-03-12 | 广西新方向化学工业有限公司 | Preparation of sugar alcohol organic selenium-rich foliar fertilizer by fermentation method |
| CN103708862A (en) * | 2013-12-31 | 2014-04-09 | 苏州科大微龙信息技术有限公司 | Ferment humic acid organic selenium fertilizer and preparation method thereof |
-
2014
- 2014-07-03 CN CN201410313901.6A patent/CN104108962A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008015334A2 (en) * | 2006-07-28 | 2008-02-07 | Ithec (Innovation Technique Expansion Commerciale) | Method for treating crop plants to enrich same with selenium and use of plants so treated |
| CN102550795A (en) * | 2011-12-26 | 2012-07-11 | 苏州硒谷科技有限公司 | Method for extracting selenium-containing protein from selenium-enriched yeast |
| CN103626587A (en) * | 2013-12-03 | 2014-03-12 | 广西新方向化学工业有限公司 | Preparation of sugar alcohol organic selenium-rich foliar fertilizer by fermentation method |
| CN103708862A (en) * | 2013-12-31 | 2014-04-09 | 苏州科大微龙信息技术有限公司 | Ferment humic acid organic selenium fertilizer and preparation method thereof |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521550A (en) * | 2014-12-01 | 2015-04-22 | 云南金雨庄生物科技有限责任公司 | Selenium-rich grifola frondosa cultivation method |
| CN105130531B (en) * | 2015-07-31 | 2018-11-20 | 中国农业科学院农产品加工研究所 | A kind of compound organism organic selenium fertilizer and its preparation method and application |
| CN105272412A (en) * | 2015-10-23 | 2016-01-27 | 广西锦翰环保科技有限公司 | Organic-Se nutrient solution for crops as well as preparation method and application of organic-Se nutrient solution |
| CN105198676A (en) * | 2015-11-09 | 2015-12-30 | 中南林业科技大学 | Selenium-rich biological organic fertilizer for camellia oleifera as well as preparation method and application of selenium-rich biological organic fertilizer |
| CN107586190A (en) * | 2017-08-03 | 2018-01-16 | 浙江省农业科学院 | A kind of tea tree charcoal base selenium-enriched fertilizer |
| CN109336687A (en) * | 2018-11-19 | 2019-02-15 | 黑龙江八农垦大学 | Hydrolyzed yeast zinc foliar fertilizer, production method and application |
| CN109485490A (en) * | 2018-11-19 | 2019-03-19 | 黑龙江八农垦大学 | Broken wall yeast zinc foliar fertilizer, production method and application |
| CN109534893A (en) * | 2018-11-19 | 2019-03-29 | 黑龙江八农垦大学 | Hydrolyzed yeast selenium foliar fertilizer, production method and application |
| CN112079658A (en) * | 2020-09-02 | 2020-12-15 | 王雪薇 | Preparation and application of plant nutrient solution rich in glucose tolerance factor |
| CN112851412A (en) * | 2021-03-01 | 2021-05-28 | 上海交通大学 | Novel organic selenium fertilizer and preparation method thereof |
| CN113383688A (en) * | 2021-06-16 | 2021-09-14 | 黑龙江植今农业科技有限责任公司 | Production method of selenium-rich rice |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104108962A (en) | Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast | |
| CN103508824A (en) | Soilless culture nutrient solution and preparation method thereof | |
| CN103319271B (en) | A kind of indoor practical straw mushroom medium and collocation method thereof | |
| US11246313B2 (en) | Synergist for improving crop stress resistance and fertilizer absorption capacity | |
| CN105585361A (en) | Selenium-enriched mushroom grass culture medium and method for producing selenium-enriched shiitake mushrooms | |
| CN103876055A (en) | Selenium-enrichment malt flour and production method thereof | |
| CN103772011B (en) | The liquid bacterial manure of a kind of cereal special composite biological | |
| CN101773229A (en) | Production method of organic selenium-enriched malt powder | |
| CN104557304A (en) | Selenium-rich shii-take cultivation material and preparation method thereof | |
| CN104115668A (en) | Production method for oyster mushrooms rich in selenium and zinc | |
| CN104892177A (en) | Special microbial manure for apple trees and preparation method thereof | |
| CN104172137A (en) | Production process of selenium-rich edible fungus powder | |
| CN106365810A (en) | Nutrient solution for soilless culture of strawberries | |
| CN103159536A (en) | Potash fertilizer comprising phosphorus decomposition and potassium decomposition factor | |
| CN103641604A (en) | Nutrient solution for water-cultured seedling of strawberry | |
| CN103288528A (en) | Seedling culture substrate for producing organic selenium-rich tomato | |
| CN102173947B (en) | Bio-organic fertilizer and preparation method thereof | |
| CN102487725A (en) | Method for culturing hypsizygus marmoreus by using corn byproduct | |
| CN103864526A (en) | Oyster mushroom dreg culture base stock and production method thereof | |
| CN107197673A (en) | The cultural method and nutrient solution of a kind of peppermint | |
| CN104774049A (en) | Organic fertilizer for crops | |
| CN104788150A (en) | Formula of special fertilizer for crop | |
| CN104788149A (en) | Preparation technology of organic fertilizer for crop | |
| CN105130581A (en) | Matrix for improving Osmunda japonica spore propagation prothallium amount | |
| CN104892100A (en) | Soil improvement formula selenium fertilizer dedicated to selenium enriched rice cultivation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141022 |