CN104068306A - Health food for improving bone density and preparation method of health food - Google Patents

Health food for improving bone density and preparation method of health food Download PDF

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Publication number
CN104068306A
CN104068306A CN201410274352.6A CN201410274352A CN104068306A CN 104068306 A CN104068306 A CN 104068306A CN 201410274352 A CN201410274352 A CN 201410274352A CN 104068306 A CN104068306 A CN 104068306A
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calcium
health food
bone density
parts
mix
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CN104068306B (en
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陈伟豪
王祝彬
吴见
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Vilnius guangsai (Guangdong) biological Polytron Technologies Inc
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GUANGZHOU SAIJIAN BIO-TECH Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses health food for improving bone density and a preparation method of the health food. The health food comprises active ingredients and auxiliary materials, wherein the active ingredients comprise the following components by weight parts: 30-48 parts of calcium acetate, 5-20 parts of casein calcium peptide, 10-35 parts of glucosamine hydrochloride, 10-30 parts of chondroitin sulfate and 1-5 parts of vitamin C. The preparation method of tablets comprises the following steps: mixing raw materials, granulating, drying, arranging granules, mixing arranged granules with vitamin C and magnesium stearate, tabletting, and coating. The health food is very high in absorption rate; by adding casein calcium peptide with a specific ratio, the calcium absorption of a human body is promoted; casein calcium peptide is short in peptide chain, small in molecular weight, good in water solubility, excellent in thermal stability and can be easily absorbed by a human body; due to casein calcium peptide with the specific ratio, very high calcium absorption capability of the human body can be kept, the calcium absorption capability of middle-aged and elderly people with relatively poor digestion-absorption function can be increased, and the bone density improvement effect is remarkable.

Description

A kind of health food that increases bone density and preparation method thereof
Technical field
The invention belongs to field of health care food, relate to a kind of health food that increases bone density and preparation method thereof.
Background technology
Osteoporosis (osteoporosis, OP) is a kind of general osteopathy that bone amount is low, bone micro-structure destroys, bone fragility increases, easily generation fracture is feature of take [1].Osteoporosis and the fracture incidence causing thereof occupy the 7th of common disease, and China's patients with osteoporosis has 6,000 ten thousand～8,000 ten thousand.Along with the aggravation of aging population and the increase of resident's life expectancy, osteoporosis with its high incidence, high disability rate, high mortality and having a strong impact on resident's life and health, becomes a serious public health problem gradually.
Modern study shows that the elderly is due to loss of tooth and gastric acid secretion deficiency or gastric anacidity, and digestive function reduces, and the ability of bone received is poor, and the picked-up of nutrition reduces, and how nutritious shortage, causes calcium, electrolytes and minerals insufficiency of intake.And China's custom meals belong to low calcium recipe, calcium source mainly relies on cereal and vegetables, the elderly's absence of tooth is more, vegetables, fruit, lean meat are difficult for chewing, intake reduces, and presents " negative calcium balance ", and feedback parathyroid hormone (PTH) secretion is risen, cause bone calcium to dissolve, blood calcium rises.Serium inorganic phosphorus content and age are obvious negative, and the elderly, because serium inorganic phosphorus reduces, increases the ratio of calcium phosphorus, causes the reduction of osteogenic action.Due to above these features, the elderly is lower to calcareous absorptivity, is osteoporosis group of people at high risk.In recent years on market, the health food or the pharmaceutical preparation that occur a large amount of preventions, improvement or auxiliary curing osteoporosis, but there is no these features for the elderly, the absorptivity of general product is not high or side effect is larger, and too much the calcareous of picked-up may be combined containing careless acid and form calcinm oxalate calculus with vegetables of taking in etc. owing to not being fully absorbed, larger to harm.Enhancing bone density product on market, one class is directly to replenish the calcium into the body of assigning to calcareous absorption, thereby reach the object that strengthens bone density, absorptivity is generally all lower, this type of strengthens bone density product has considerable part all to adopt calcium carbonate etc. need to consume the calcareous of hydrochloric acid in gastric juice, poorly water-soluble, be unfavorable for absorbing, and can make the elderly's hydrochloric acid in gastric juice more lack.One class is to supplement calcium or do not supplement calcium, and by adjusting endocrine, promote the absorption of calcium, this type of strengthens bone density product and adopts the drug ingedients such as estrogen, Chinese medicine, and the elderly poor for physique, endocrine disturbance easily occurs is also improper, has certain side effect.One class is supplement calcium, supplement sorbefacient simultaneously, this type of strengthens bone density product and is selecting under suitable calcareous and prerequisite that optimization of C/C composites forms, absorptivity is higher, but because the suitable calcium source of pin the elderly's not feature selecting and calcium promote absorbent, as a lot of enhancing bone density products, adopt the poorly water-solubles such as calcium carbonate, consume the calcareous of hydrochloric acid in gastric juice; As adopt vitamin D as accelerating agent of calcium absorption, because the metabolism time of vitamin D in human body is longer, and a lot of the elderly's metabolic function is relatively poor, easily accumulates in vivo, causes poisoningly, more harm than good equally, and side effect easily occurs.Therefore, need a kind of with strong points, absorptivity is high, have no side effect, be applicable to the enhancing bone density product that the elderly takes.
CPP (CPP), is to take bovine casein as raw material, and what by biotechnology, make has a bioactive polypeptide.Experimental results demonstrate that CPP can effectively promote the absorption of human body to calcium.Chinese patent (CN102178933A) discloses a kind of preparation for Prevention and Treatment of Osteoporosis and osteoarthropathy, wherein adopts calcium carbonate, aminoglucose hydrochloride, chondroitin sulfate, CPP, ossein, neo dohyfral D3 isoreactivity composition.Chinese patent (CN102133395A) discloses a kind of skeleton health-care product or pharmaceutical composition and preparation method thereof, said composition comprises calcium carbonate, neo dohyfral D3, CPP, chondroitin sulfate, aminoglucose hydrochloride and ossein isoreactivity composition and alleviates bone pain recovery bone function raising immunity for increasing bone density, obviously solves the symptoms such as osteoporosis.Chinese patent (CN101904866A) discloses a kind of osteoporotic pharmaceutical composition and preparation method thereof for the treatment of, and said composition contains D-Glucosamine Hydrochloride, calcium carbonate, neo dohyfral D3 and chondroitin sulfate isoreactivity composition.Chinese patent (CN101322551A) discloses a kind of for strengthening the composition of bone density and repairing articular cartilage, and wherein said composition contains chondroitin sulfate, Glucosamine and calcium agent isoreactivity composition.As can be seen here, CPP (CPP) is the active component of common increase bone density.
Through large quantity research, discovery CCP (CCP) is compared and can more effectively be promoted human body to the combination of calcium, absorption with CPP (CPP).And through formula screening, select the CCP (CCP) of specified quantitative, its formula effect is very obvious, and effect significantly.CCP (CCP) is a kind of phosphoric acid silk amino acid residue biologically active polypeptide that contains equally, is caseic calcium salt (calcium caseinate) refining forming after enzyme hydrolysis in cow's milk.When CCP enters after small intestine, the phosphorus in structure can be combined and make calcium keep solvable state with the calcium of little ilium, to promote the absorption of calcium and the formation of sclerotin.The peptide chain of CCP (CCP) for being formed by phosphoserine in casein, peptide chain average molecular mass is less by approximately 3000, and peptide chain is shorter, soluble in water, is conducive to absorption of human body.Can more effectively promote the absorption of human body to calcium, guarantee higher calcium absorptivity.In addition, CCP (CCP) heat endurance is strong, at 120 ℃ of aqueous solution heating, 30min, without precipitation, can guarantee, under high temperature reconstitutes mode, to keep its activity.CCP (CCP) and CPP (CPP) can promote the absorption of calcium equally, effect is suitable, CCP (CCP) is compared with CPP (CPP), peptide chain is shorter, molecular weight is less, water-soluble better, heat endurance is stronger.Therefore in increasing bone density health food, the alternative CPP (CPP) of available CCP (CCP) can more effectively promote human body to the combination of calcium, absorption.CCP (CCP) safe without toxic side effect in addition.Therefore, CCP (CCP) can apply to strengthen in the health food of bone density, strengthens the elderly to calcareous absorption.The application that CCP (CCP) strengthens bone density product the elderly is less.Chinese patent (CN102824627A) discloses a kind of snake bone calcium capsule pulvis and preparation method thereof, and snake bone calcium capsule pulvis contains that the compositions such as pallas pit viper vertebrae, black high and steep snake vertebrae, CCP can be used for course of therapy and prevention of osteoporosis is evident in efficacy.But in recent years, find to come supplement calcium easily to cause arsenic poisoning with Animal Bone, be not suitable for the elderly, have certain risk.
Summary of the invention
The present invention is in order to solve above-described variety of issue and defect, the invention provides a kind of with strong points, absorptivity is high, have no side effect, be applicable to the enhancing bone density product that the elderly takes.
The object of the present invention is to provide a kind of health food that increases bone density.
Another object of the present invention is to provide a kind of preparation method who increases the health food of bone density.
The technical solution used in the present invention is:
Increase a health food for bone density, active ingredient and auxiliary material, consist of, its active ingredient is comprised of the raw material of following parts by weight: 30～48 parts of calcium acetates; 5～20 parts of CCPs; 10～35 parts of aminoglucose hydrochlorides; 10～30 parts of chondroitin sulfates; 1～5 part of vitamin C.
Further, a kind of health food that increases bone density, is comprised of active ingredient and auxiliary material, and its active ingredient is comprised of the raw material of following parts by weight: 44 parts of calcium acetates; 8 parts of CCPs; 20 parts of aminoglucose hydrochlorides; 10 parts of chondroitin sulfates; 3 parts of vitamin Cs.
Further, the formulation of above-mentioned health food is tablet, pulvis, granule, medicinal tea, hard shell capsules, soft capsule or oral liquid.
Further, above-mentioned auxiliary material is gross weight 1～35%, and it is selected from least one in filler, adhesive, sweetener, acid, flavoring essence, food coloring, lubricant, anticorrisive agent, suspending agent, diluent and plasticizer, antioxidant.
Further, above-mentioned filler is selected from least one in fructose, antierythrite, hydroxyl isomaltulose, sweet mellow wine, starch, dextrin;
Above-mentioned adhesive is selected from least one in hydroxypropyl methylcellulose, pregelatinized starch, PVP;
Above-mentioned sweetener be selected from Sucralose, steviol glycoside, 3,4-Dihydro-6-methyl-1,2,3-oxathiazin-4-one 2,2-dioxide potassium salt, Aspartame, mogroside, knob sweet at least one;
Above-mentioned acid is selected from least one in citric acid, lactic acid, tartaric acid, fumaric acid;
Above-mentioned flavoring essence is selected from least one in orange essence, lemon extract, strawberry essence, blueberry essence, flavoring apple essence;
Above-mentioned food coloring is selected from least one in burnt sugar coloring, lemon yellow pigment, light blue pigment, carmine pigment, sunset yellow, allured red pigment, ginger color, Gardenia Yellow;
Above-mentioned lubricant is selected from least one in talcum powder, dolomol, silica, stearic acid, magnesium fumarate;
The above-mentioned anticorrisive agent of stating is selected from least one in Sodium Benzoate, benzoic acid, sorbic acid, potassium sorbate, metagin class;
Above-mentioned suspending agent is selected from least one in sodium carboxymethylcellulose, sodium alginate, polyethylene glycol, PVP K30, beeswax;
Above-mentioned diluent is selected from least one in water, edible vegetable oil, polyethylene glycol;
Above-mentioned plasticizer is selected from least one in glycerine, sorbierite;
Above-mentioned antioxidant is selected from least one in vitamin C, vitamin E etc., Co-Q10, beta carotene, lutein, epiphysin, luteole, angle xanthin, tuna flavine, astaxanthin, haematococcus pluvialis extract.
The preparation method of tablet who increases the health food of bone density, comprises the following steps:
1) by above-mentioned formula, take each raw material;
2) mix: after CCP, pregelatinized starch are fully mixed, then add calcium acetate, aminoglucose hydrochloride, chondroitin sulfate, mix;
3) granulate: the ethanolic solution that is 40～60% by volumetric concentration spray in above-mentioned mixed powder until hold mixture can be agglomerating, finger is light presses loosely, in every kilogram of mixed powder, sprays 0.1～0.20kg volumetric concentration and be 40～60% ethanolic solution; Then with 16 eye mesh screens, granulate;
4) dry: the wet granular that previous step is made is dried, and baking temperature is 55～65 ℃, when pellet moisture content is 3～4.5%, takes out dry particle, is cooled to room temperature;
5) whole grain: dried dry particle is crossed to 16 mesh sieves with pelletizing machine and carry out whole grain;
6) always mixed: first the part particle after whole grain to be increased progressively and mixed with vitamin C equivalent, then add particle and the dolomol after the whole grain of residue, mix, obtain always mixed material;
7) compressing tablet: will always mix material and carry out obtaining plain sheet after compressing tablet with tablet press machine;
8) dressing: Hydroxypropyl methylcellulose and Macrogol 4000 are made into the film coating liquid of 10% (w/v), more plain sheet is carried out to dressing with coating solution in coating pan, gained coating tablet is the health food that increases bone density.
The invention has the beneficial effects as follows:
1. crucial innovative point of the present invention is that product absorptivity of the present invention is very high, by adding the CCP of specific proportioning, promote the absorption of human body to calcium, CCP peptide chain is short, molecular weight is little, good water solubility, heat endurance are strong, be easy to absorption of human body, the calcium caseinate Toplink of specified quantitative makes the absorbability that human body is very high to calcareous maintenance, can make weak the elderly of digestion and absorption function originally strengthen calcareous absorbability, the absorptivity very high to calcareous maintenance, strengthens bone density successful.
2. crucial innovative point of the present invention is that product of the present invention is with strong points.The elderly that the present invention is gastric anacidity from the feature of middle-aged and old physique etc. provides formula more targetedly, makes the elderly unaffected to the absorption effect of calcium, better reaches the effect that strengthens bone density.
The present invention formula safely, have no side effect, without medicine, Animal Bone etc., bring side effect.
4. the present invention takes stopgap measures and effects a permanent cure, and by combining, carrys out the syndromes such as ostalgia that releasing osteoporosis brings, inflammation with the conventional safe drugs composition chondroitin sulfate of osteoporosis, aminoglucose hydrochloride.In the time of for the elderly's prevention or releasing osteoporosis disease, effectively lower the senses of discomfort such as pain.
The present invention pointed strong, absorptivity is high, have no side effect, take stopgap measures and effect a permanent cure, be applicable to the feature that the elderly takes.
6. its composition of mainly filling a prescription of the present invention comprises calcium acetate, CCP, chondroitin sulfate, aminoglucose hydrochloride.The present invention carrys out the picked-up of specific aim strengthening the elderly to calcium by not consuming the calcium acetate of hydrochloric acid in gastric juice, good water solubility as calcium source, by adding the CCP of specified quantitative to promote the absorption of the elderly to calcium, guarantee higher calcium absorptivity, calcium acetate and CCP synergy increase bone density; And combine with osteoporosis common drug composition chondroitin sulfate, aminoglucose hydrochloride and carry out the syndromes such as ostalgia that releasing osteoporosis brings, inflammation.The present invention takes stopgap measures and effects a permanent cure, pointed strong, absorptivity is high, have no side effect, be applicable to the feature that the elderly takes.Be exactly more than the good technique effect that the present invention produces, for the elderly's prevention or releasing osteoporosis disease, strengthen bone density.
The specific embodiment
Increase a health food for bone density, its raw material by following parts by weight is made: active ingredient and auxiliary material, consist of, its active ingredient is comprised of the raw material of following parts by weight: 30～48 parts of calcium acetates; 5～20 parts of CCPs; 10～35 parts of aminoglucose hydrochlorides; 10～30 parts of chondroitin sulfates; 1～5 part of vitamin C.
Preferably, a kind of health food that increases bone density, is comprised of active ingredient and auxiliary material, and its active ingredient is comprised of the raw material of following parts by weight: 44 parts of calcium acetates; 8 parts of CCPs; 20 parts of aminoglucose hydrochlorides; 10 parts of chondroitin sulfates; 3 parts of vitamin Cs.
Increase a health food for bone density, its formulation is tablet, pulvis, granule, medicinal tea, hard shell capsules, soft capsule or oral liquid.
Preferably, described auxiliary material is gross weight 1～35%, and it is selected from least one in filler, adhesive, sweetener, acid, flavoring essence, food coloring, lubricant, anticorrisive agent, suspending agent, diluent and plasticizer, antioxidant.
Described filler is preferably at least one in fructose, antierythrite, hydroxyl isomaltulose, sweet mellow wine, starch, dextrin;
Described adhesive is preferably at least one in hydroxypropyl methylcellulose, pregelatinized starch, PVP;
Described sweetener be preferably Sucralose, steviol glycoside, 3,4-Dihydro-6-methyl-1,2,3-oxathiazin-4-one 2,2-dioxide potassium salt, Aspartame, mogroside, knob sweet at least one;
Described acid is preferably at least one in citric acid, lactic acid, tartaric acid, fumaric acid;
Described flavoring essence is preferably at least one in orange essence, lemon extract, strawberry essence, blueberry essence, flavoring apple essence;
Described food coloring is preferably at least one in burnt sugar coloring, lemon yellow pigment, light blue pigment, carmine pigment, sunset yellow, allured red pigment, ginger color, Gardenia Yellow;
Described lubricant is preferably at least one in talcum powder, dolomol, silica, stearic acid, magnesium fumarate;
Described anticorrisive agent is preferably at least one in Sodium Benzoate, benzoic acid, sorbic acid, potassium sorbate, metagin class;
Described suspending agent is preferably at least one in sodium carboxymethylcellulose, sodium alginate, polyethylene glycol, PVP K30, beeswax;
Described diluent is preferably at least one in water, edible vegetable oil, polyethylene glycol;
Described plasticizer is preferably at least one in glycerine, sorbierite;
Described antioxidant is preferably at least one in vitamin C, vitamin E etc., Co-Q10, beta carotene, lutein, epiphysin, luteole, angle xanthin, tuna flavine, astaxanthin, haematococcus pluvialis extract.
The preparation method of tablet who increases the health food of bone density, comprises the following steps:
Step 1: take each raw material by above-mentioned formula;
Step 2: mix
After CCP, pregelatinized starch are fully mixed, then add calcium acetate, aminoglucose hydrochloride, chondroitin sulfate, mix.
Step 3: granulate
The ethanolic solution that is 40～60% by volumetric concentration spray in above-mentioned mixed powder until hold mixture can be agglomerating, finger is light presses loosely, in every kilogram of mixed powder, sprays 0.1～0.20kg volumetric concentration and be 40～60% ethanolic solution; Then with 16 eye mesh screens, granulate.
Step 4: dry
The wet granular that previous step is made is dried, and baking temperature is 55～65 ℃, when pellet moisture content is 3～4.5%, takes out dry particle, is cooled to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with pelletizing machine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, then add remainder particulate and dolomol, mix, obtain always mixed material.
Step 7: compressing tablet
To always mix material carries out obtaining plain sheet after compressing tablet with tablet press machine.
Step 8: dressing
Hydroxypropyl methylcellulose and Macrogol 4000 are made into the film coating liquid of 10% (w/v), more plain sheet is carried out to dressing with coating solution in coating pan, gained coating tablet is the health food that increases bone density.
The using method of the increase bone density health food of casein containing protein calcium peptide: a specification 1g/ sheet, every day twice, sooner or later respectively once, once two, oral.
Below in conjunction with specific experiment example, the present invention is further illustrated, but be not limited to this.
Embodiment 1:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 44%; CCP 8%; Aminoglucose hydrochloride 20%; Chondroitin sulfate 10%; Vitamin C 3%; Pregelatinized starch 12%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: take raw material
Meter takes following raw material by weight: 44 parts of calcium acetates; 8 parts of CCPs; 20 parts of aminoglucose hydrochlorides; 10 parts of chondroitin sulfates; 3 parts of vitamin Cs; 12 parts of pregelatinized starch; 1 part of dolomol; 1.5 parts of Hydroxypropyl methylcelluloses; 0.5 part of Macrogol 4000.
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix and mix for 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By volumetric concentration be 50% ethanolic solution spray in above-mentioned mixed powder until hold mixture can be agglomerating, finger is light presses loosely, in every kilogram of mixed powder, sprays 0.1～0.20kg concentration and be 50% ethanolic solution; Then with oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
The wet granular that previous step is made is placed in CTCI heated-air circulation oven and is dried, and it is 55～65 ℃ that baking temperature is controlled, and when pellet moisture content is 3～4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
Particle after the whole grain of vitamin C and equivalent is mixed, then add remainder particulate and dolomol, and be placed in three-dimensional motion mixer always mixed 20 minutes, it is fully mixed, obtain always mixed material.
Step 7: compressing tablet
To always mix material carries out obtaining plain sheet after compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
Film coating material Hydroxypropyl methylcellulose and Macrogol 4000 are slowly joined to the film coating liquid that is made into 10% (w/v) in the purified water under stirring, again plain sheet is added in coating pan, open hot blast, preheating 10min, start coating pan, retention tab bed tempertaure sprays into coating solution at 40～45 ℃.Gained coating tablet is the health food of increase bone density of the present invention.
Embodiment 2:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 42.3%; CCP 7.5%; Aminoglucose hydrochloride 22%; Chondroitin sulfate 17%; Catergen .8%; Pregelatinized starch 2%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 3:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 44.8%; CCP 8.4%; Aminoglucose hydrochloride 23%; Chondroitin sulfate 16%; Catergen .8%; Pregelatinized starch 2%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 4:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 40%; CCP 9.5%; Aminoglucose hydrochloride 19%; Chondroitin sulfate 20%; Catergen .2%; Pregelatinized starch 8.3%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 5:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 47.6%; CCP 5%; Aminoglucose hydrochloride 22%; Chondroitin sulfate 18%; Catergen .4%; Pregelatinized starch 2%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 6:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 30%: CCP 7.5%; Aminoglucose hydrochloride 35%; Chondroitin sulfate 18%; Vitamin C 4%; Pregelatinized starch 2.5%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 7:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 48%; CCP 6.8%; Aminoglucose hydrochloride 23.2%; Chondroitin sulfate 14%; Vitamin C 3%; Pregelatinized starch 2%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 8:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 41%; CCP 8.3%; Aminoglucose hydrochloride 23%; Chondroitin sulfate 17%; Catergen %; Pregelatinized starch 5.7%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
Embodiment 9:
An increase bone density health food for casein containing protein calcium peptide, its primary raw material formula is by weight percentage as follows: calcium acetate 44%; CCP 8%; Aminoglucose hydrochloride 25%; Chondroitin sulfate 15%; Vitamin C 3%; Pregelatinized starch 2%; Dolomol 1%; Hydroxypropyl methylcellulose 1.5%; Macrogol 4000 0.5%.
A preparation method for the increase bone density health food of casein containing protein calcium peptide, comprises the following steps:
Step 1: weigh
Step 2: mix
CCP, pregelatinized starch are placed in trough type mixing machine, mix 10 minutes, then calcium acetate, aminoglucose hydrochloride, chondroitin sulfate are dropped in trough type mixing machine, mix 10 minutes, mix.
Step 3: granulate
By 50% ethanolic solution, spray into softwood processed in above-mentioned mixed powder, until that softwood is held is agglomerating, finger is light press loose; With oscillating granulator, filling 16 eye mesh screens granulates.
Step 4: dry
Wet granular is placed in to CTCI heated-air circulation oven and is dried, it is 55 ℃～65 ℃ that baking temperature is controlled, and pellet moisture is controlled at 3%-4.5%, takes out dry particle, lets cool to room temperature.
Step 5: whole grain
Dried dry particle is crossed to the whole grain of 16 mesh sieves with Fastgranulatemachine.
Step 6: always mixed
First the part particle after whole grain is increased progressively and mixed with vitamin C equivalent, more always mix 20 minutes with remainder particulate, dolomol and the above-mentioned three-dimensional motion mixer that is placed in, obtain always mixed material.
Step 7: compressing tablet
To always mix material and carry out compressing tablet with Highspeedrotarytabletpress.
Step 8: dressing
By film coating material Hydroxypropyl methylcellulose and Macrogol 4000, slowly join the film coating liquid that is made into 10% in the purified water under stirring.Plain sheet is added in coating pan again, open hot blast, preheating 10min, starts coating pan, and retention tab bed tempertaure sprays into coating solution at 40-45 ℃.Coating tablet be the increase bone density tablet health food of a kind of casein containing protein calcium of the present invention peptide.
The health food of the increase bone density of below being prepared by the present invention is made further effect detection.
One, the impact on bone density, bone mass and calcium content of bone
1 materials and methods
1.1 animals used as test: 110 of the clean level female sd inbred rats of weight (301.2 ± 6.9) g.
The sample (1g/ sheet) that 1.2 tested materials: embodiment 1～8 is prepared, human oral RD is every day 2 times, each two, become body mass press 60kg calculating, amounting to dosage is 0.0667gkg -1.
1.3 instruments: SD-1000c bone mineral measuring instrument
1.4 method
With reference to the method for FDA Food and Drug Administration (FDA) and the World Health Organization (WHO) recommendation research osteoporosis, i.e. castration method modeling.The modeling of castration method is the best approach of the research osteoporosis of FDA Food and Drug Administration (FDA) and the World Health Organization (WHO) recommendation.
1.4.1 dosage is selected to give mode with tested material
13 groups are established in experiment: sham-operation group, solvent control group (oophorectomize+solvent control group), calcium carbonate control group (oophorectomize+calcium carbonate control group), calcium acetate control group (oophorectomize+calcium acetate control group), calcium caseinate control group (oophorectomize+calcium caseinate control group), 1 group of embodiment (oophorectomize+embodiment 1 sample sets), 2 groups of embodiment (oophorectomize+embodiment 2 sample sets), 3 groups of embodiment (oophorectomize+embodiment 3 sample sets), 4 groups of embodiment (oophorectomize+embodiment 4 sample sets), 5 groups of embodiment (oophorectomize+embodiment 5 sample sets), 6 groups of embodiment (oophorectomize+embodiment 6 sample sets), 7 groups of embodiment (oophorectomize+embodiment 7 sample sets), 8 groups of embodiment (oophorectomize+embodiment 8 sample sets).
According to the oral recommended amounts of human body, establish low middle high dose and be respectively 0.33,0.66,2.00gkg -1(be equivalent to respectively human body RD 5,10,30 times), test dose select in dosage 0.66gkg -1.
During sample preparation, embodiment group: get respectively corresponding embodiment sample 6.6g, grind, add deionized water to 100mL;
Calcium acetate control group: in the sample formula of hello use,, except not containing CCP, it is the same with embodiment 1 formula that other compositions are, and the quantity reducing according to CCP, the quantity of pregelatinized starch in corresponding adjustment formula, sample thief 6.6g, grinds, and adds deionized water to 100mL;
CCP control group, in the sample formula of hello use, except not containing calcium acetate, it is the same with embodiment 1 formula that other compositions are, and the quantity reducing according to calcium acetate, the quantity of pregelatinized starch in corresponding adjustment formula, sample thief 6.6g, grind, add deionized water to 100mL;
Calcium carbonate control group: per os gives 0.50gkg -1calcium carbonate, get 5g calcium carbonate (calcium content is 40%) and add deionized water to 100mL;
Sham-operation group and solvent control group: give isopyknic deionized water, give respectively animal subject gavage, every day gavage once, every 100g weight rat oral gavage volume is 1.0mL.
From on-test, each treated animal all single cage is raised, and feeds and raises identical feed, and gavage gives the test solution that is subject to separately, drinks deionized water, records food-intake.Totally 12 weeks.
Above-mentioned feed formula (mass percent): low calcium casein 23.0%, DL-METHIONINE 0.3%, cornstarch 32.0%, sucrose 30.0%, fiber 5.0%, mixed mineral salt 3.5%, mixed vitamin 1.0%, two choline tartrate 0.2%.
1.4.2 oophorectomize: rat is with 30mgkg -1dosage lumbar injection 1% amobarbital sodium solution, after anesthesia, carry out Bilateral oophorectomy, the penicillin of postoperative muscle injection 20,000 units, continuously 3d.
Sham-operation group: only excise the fat about 0.5g after opening abdominal cavity, retain bilateral ovaries.5d after rat ovary excision, carries out vaginal smear examination, rejects the incomplete rat of oophorectomize.
1.4.3 animal grouping: above-mentioned female castrated rats is divided into 1～9 group, sham-operation group, solvent control group, calcium carbonate control group, calcium acetate control group, calcium caseinate control group and embodiment sample at random by weight, amount to 13 groups, every group 10, single cage is fed, and feeds 12 weeks.
1.4.4 growth of animal index Weighing body quality and height weekly.
1.4.5 femur dry weight and bone densitometry: when experiment finishes, rat is put to death in cervical vertebra dislocation, peels off right femur, through 105 ℃, toast 8h to constant weight, be cooled to after room temperature, weigh key heavy and bone length, and measure the bone density (BMD) of femur midpoint and distal end.
1.4.6 calcium content of bone is measured: weigh after the gross mass of femur, through high-temperature baking, femur is made to bone ash, use 6molL -1after dissolving with hydrochloric acid, with EDTA titration measuring calcium content of bone.
1.4.7 statistical procedures method: carry out variance analysis with SPPS (11.0) software, result represents with mean ± standard deviation
2 results
2.1 impacts of health product of the present invention on rat weight
Body weight to the rat after above-mentioned different disposal is measured, and measurement result, as shown in table 1 and continued 1, can be found out the inchoate aspect mass discrepancy not statistically significant (P > 0.05) of respectively organizing rat before experiment from table 1 and continued 1.Difference between the body weight of respectively organizing rat of latter the 4th week of experiment, the 8th weekend, experiment latter stage (the 12nd weekend) is also not obvious, not statistically significant (P > 0.05).
According to the body weight of table different disposal group rat, calculate and respectively organize the weight gain situation of rat when latter stage (the 12nd weekend) is tested in processing, result of calculation is as shown in continued 1, respectively organize as can be seen from the table the weight gain difference of rat when latter stage (the 12nd weekend) is tested in processing not obvious, not statistically significant (P > 0.05).
The impact of table 1 different disposal on rat body weight
Note: with the comparison of solvent control group, *p > 0.05.
The impact of continued 1 different disposal on rat body weight
Note: with model control group comparison, *p > 0.05.
2.2 impacts of health product of the present invention on the length of rat femur and bone density
To processing length and the bone density of the femur of the rat in (experiment latter stage) after 12 weeks, measure, measurement result is as shown in table 2, therefrom can find out the femur length difference not statistically significant (P > 0.05) of respectively organizing rat.
In bone density measurement result (table 2), the femur center bone density of sham-operation group and calcium carbonate control group, femur distal end bone density are all higher than solvent control group, but difference is not remarkable, not statistically significant (P > 0.05);
The femur center bone density of calcium acetate control group and calcium caseinate control group and the difference of solvent control group are not obvious, not statistically significant (P > 0.05); And this femur distal end bone density of two groups is all higher than solvent control group, there is significant difference, P < 0.05;
The rat femur center bone density of each embodiment group, femur distal end bone density, all higher than solvent control group, have significant difference, P < 0.05; But compare with solvent control group, only has the rat femur center bone density of 1 group of embodiment, the amplitude of femur distal end bone density increase is maximum.
The above results explanation, only have the health food of increase bone density of the present invention rat femur center bone density and femur distal end bone density all to be there is to the effect of increase, obviously be better than the effect of other each control groups, and there is best increase bone density effect with the health food that in embodiment 1 prepared by specific proportioning.
The impact of table 2 different disposal on rat femur length and bone density
Note: with the comparison of solvent control group, *p < 0.05, *p < 0.01.
2.3 impacts of health product of the present invention on rat femur quality and calcium content of bone
To processing femoral shaft quality and the bone calcium of the rat in (experiment latter stage) after 12 weeks, measure, measurement result is as shown in table 3.
The impact of table 3 different disposal on rat femur quality and calcium content of bone
Note: with model control group comparison, *p < 0.05, *p < 0.01.。
As can be seen from Table 3:
Femoral shaft quality, the calcium content of bone of sham-operation group all, higher than solvent control group, have significant difference, P < 0.05;
The femoral shaft quality of calcium carbonate control group and calcium caseinate control group and the difference of solvent control group are not obvious, not statistically significant (P > 0.05); And this calcium content of bone of two groups is higher than solvent control group, there is significant difference, P < 0.05;
Femoral shaft quality, the calcium content of bone of calcium acetate control group all, higher than solvent control group, have significant difference, P < 0.05;
Rat femur dry mass, the calcium content of bone of each embodiment group all, higher than solvent control group, have significant difference, P < 0.05; But only have the rat bone calcium content of 1 group of embodiment to be more significantly higher than solvent control group, there is utmost point significant difference, P < 0.01.
The above results explanation, the health food of increase bone density of the present invention all has the effect of increase to rat femur dry mass, calcium content of bone, the effect that is better than calcium carbonate control group, calcium caseinate control group and calcium acetate control group, and with the health food that in embodiment 1 prepared by specific proportioning, there is the effect of best lifting rat bone calcium content.Illustrate that embodiment 1 sample increases rat bone density effect more obviously, effectively.
From above-mentioned experiment, the product of the present invention that main formula is made for calcium acetate, CCP, aminoglucose hydrochloride, chondroitin sulfate has the function of enhancing degree bone density, and the formula of specific proportioning composition, and its effect more obviously, effectively.
Two, calcium absorption experiment
1. principle
Under stable state, measure the calcium amount of discharging in calcium amount that rat takes in and ight soil, both differences are apparent absorption rate (%)=(the taking in calcium-excrement calcium) of the calcium calcium of apparent absorption/take in calcium * 100%.
2. instrument and reagent
Instrument: metabolism mouse cage, animal balance, atomic absorption spectrophotometer (tool flame method determinator deuterium lamp background deduction);
Reagent: perchloric acid (top grade is pure);
2% lanthana solution: take and analyze pure zirconia lanthanum (content > 99.99%) 25 grams, add 75% top grade pure hydrochloric acid, add deionized water to 1000ml;
Calcium standard solution: take 1.2486 grams and analyze calcium carbonate (purity is greater than 99.99%), add 50ml deionized water, add hydrochloric acid and make it to dissolve.Move in 1000ml volumetric flask, add 2% lanthana solution to scale.This solution 1.00ml is equivalent to 500ug calcium.Be stored in polyethylene bottle, 4 ℃ of preservations, face used time dilution.
3 experimental techniques
3.1 animals used as test: be born about 4 weeks ablactation rat.
3.2 basal feeds: low calcium formula (mass percent): low calcium casein 23.0%, DL-METHIONINE 0.3%, cornstarch 32.0%, sucrose 30.0%, fiber 5.0%, mixed mineral salt 3.5%, mixed vitamin 1.0%, two choline tartrate 0.2%.
3.3. zoopery grouping and dosage setting
Three dosage groups are established in experiment, and low middle high dose is respectively 0.33,0.66,2.00gkg -1(be equivalent to respectively human body RD 5,10,30 times), establish low calcium control group (150mg/100g feed) and the identical calcium carbonate control group of tested material level corresponding with dosage group simultaneously.By low calcium basal feed preparation given the test agent each dosage group and calcium carbonate control group.
Low calcium basal feed, the i.e. feed in low calcium source, as food source, also as the tested material of low calcium control group, when tested material cannot gavage, feed can be used as the carrier of other given the test agent of gavage, and tested material is mixed in feed, is used for preparing each dosage group of given the test agent and each dosage calcium carbonate control group.
Low calcium control group: add appropriate amount of deionized water with low calcium basal feed, per os gives gavage
1 group of basic, normal, high dose delivery example: the quantity according to starch in the low calcium basal feed of the corresponding adjustment of the quantity of the given the test agent mixing, add appropriate amount of deionized water, per os gives gavage
Basic, normal, high dosage calcium carbonate control group: the quantity according to starch in the low calcium basal feed of the corresponding adjustment of the quantity of the calcium carbonate mixing, add appropriate amount of deionized water, per os gives gavage.
3.4 metabolism
Birth surrounding ablactation rat after one week laundering period, sub-cage rearing 4 weeks.Every group has do not move 8 of things of homogeneity.Drink deionized water, test and after 3 weeks, carry out calcium metabolism experiment in 3 days.Record 3 days food-intakes, collect 72 hours ight soil, measure calcium content in feed and ight soil.
The assay method of calcium in 3.5 fodder machine ight soil: atomic absorption spectrophotometry is measured.
3.6 calculate
Take in calcium (mg/d)=Calcium Content in Foodstuff (mg/g) * feed consumption (g/d)
Calcium content (mg/g) * ight soil discharge rate (g/d) in excrement calcium (mg/d)=ight soil
3.7 data processing
Statistical procedures for method SPPS (11.0) software carry out variance analysis, result represents with mean ± standard deviation
4 results
Test calculating embodiment 1 product calcium apparent absorption rate after 3 weeks, result of calculation is as shown in table 4, therefrom can find out that each dosage group apparent absorption rate of embodiment 1 sample and low calcium control group difference are little, the calcium carbonate control group of each dosage group apparent absorption rate of embodiment 1 sample and same calcium absorption level has significant difference (p < 0.05), each dosage group apparent absorption rate of embodiment 1 sample is all significantly higher than par calcium carbonate group, the results are shown in Table 4.
Table 4 embodiment 1 sample calcium apparent absorption rate
Note: with the comparison of low dosage calcium carbonate control group, *p < 0.05; With the comparison of middle dosage calcium carbonate control group, *p < 0.05; With the comparison of high dose calcium carbonate control group, * *p < 0.05.
The formula of experimental result prompting embodiment 1 its specific proportioning of sample forms, and its calcium absorption efficiency is high.Solution provided by the invention, provides calcium acetate, CCP, the aminoglucose hydrochloride of certain content, the health food of chondroitin sulfate, has the health care of remarkable enhancing degree density.

Claims (6)

1. increase a health food for bone density, by active ingredient and auxiliary material, formed, it is characterized in that: its active ingredient is comprised of the raw material of following parts by weight: 30～48 parts of calcium acetates; 5～20 parts of CCPs; 10～35 parts of aminoglucose hydrochlorides; 10～30 parts of chondroitin sulfates; 1～5 part of vitamin C.
2. increase a health food for bone density, by active ingredient and auxiliary material, formed, it is characterized in that: its active ingredient is comprised of the raw material of following parts by weight: 44 parts of calcium acetates; 8 parts of CCPs; 20 parts of aminoglucose hydrochlorides; 10 parts of chondroitin sulfates; 3 parts of vitamin Cs.
3. a kind of health food that increases bone density according to claim 1 and 2, is characterized in that: its formulation is tablet, pulvis, granule, medicinal tea, hard shell capsules, soft capsule or oral liquid.
4. a kind of health food that increases bone density according to claim 1 and 2, it is characterized in that: described auxiliary material is gross weight 1～35%, it is selected from least one in filler, adhesive, sweetener, acid, flavoring essence, food coloring, lubricant, anticorrisive agent, suspending agent, diluent and plasticizer, antioxidant.
5. a kind of health food that increases bone density according to claim 4, is characterized in that:
Described filler is selected from least one in fructose, antierythrite, hydroxyl isomaltulose, sweet mellow wine, starch, dextrin;
Described adhesive is selected from least one in hydroxypropyl methylcellulose, pregelatinized starch, PVP;
Described sweetener be selected from Sucralose, steviol glycoside, 3,4-Dihydro-6-methyl-1,2,3-oxathiazin-4-one 2,2-dioxide potassium salt, Aspartame, mogroside, knob sweet at least one;
Described acid is selected from least one in citric acid, lactic acid, tartaric acid, fumaric acid;
Described flavoring essence is selected from least one in orange essence, lemon extract, strawberry essence, blueberry essence, flavoring apple essence;
Described food coloring is selected from least one in burnt sugar coloring, lemon yellow pigment, light blue pigment, carmine pigment, sunset yellow, allured red pigment, ginger color, Gardenia Yellow;
Described lubricant is selected from least one in talcum powder, dolomol, silica, stearic acid, magnesium fumarate;
Described anticorrisive agent is selected from least one in Sodium Benzoate, benzoic acid, sorbic acid, potassium sorbate, metagin class;
Described suspending agent is selected from least one in sodium carboxymethylcellulose, sodium alginate, polyethylene glycol, PVP K30, beeswax;
Described diluent is selected from least one in water, edible vegetable oil, polyethylene glycol;
Described plasticizer is selected from least one in glycerine, sorbierite;
Described antioxidant is selected from least one in vitamin C, vitamin E etc., Co-Q10, beta carotene, lutein, epiphysin, luteole, angle xanthin, tuna flavine, astaxanthin, haematococcus pluvialis extract.
6. the preparation method of the tablet of the arbitrary described a kind of health food that increases bone density of claim 1～5, is characterized in that: comprise the following steps:
1) by formula claimed in claim 1, take each raw material;
2) mix: after CCP, pregelatinized starch are fully mixed, then add calcium acetate, aminoglucose hydrochloride, chondroitin sulfate, mix;
3) granulate: the ethanolic solution that is 40～60% by volumetric concentration spray in above-mentioned mixed powder until hold mixture can be agglomerating, finger is light presses loosely, in every kilogram of mixed powder, sprays the ethanolic solution that 0.1～0.20kg concentration volume is 40～60%; Then with 16 eye mesh screens, granulate;
4) dry: the wet granular that previous step is made is dried, and baking temperature is 55 ~ 65 ℃, when pellet moisture content is 3～4.5%, takes out dry particle, is cooled to room temperature;
5) whole grain: dried dry particle is crossed to 16 mesh sieves with pelletizing machine and carry out whole grain;
6) always mixed: first the part particle after whole grain to be increased progressively and mixed with vitamin C equivalent, then add particle and the dolomol after the whole grain of residue, mix, obtain always mixed material;
7) compressing tablet: will always mix material and carry out obtaining plain sheet after compressing tablet with tablet press machine;
8) film coating liquid dressing: Hydroxypropyl methylcellulose and Macrogol 4000 are made into 10%(w/v), more plain sheet is carried out to dressing with coating solution in coating pan, gained coating tablet is the health food that increases bone density.
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CN104273541A (en) * 2014-10-29 2015-01-14 成都健怡营销策划有限公司 Health product for increasing bone mineral density
CN104543674A (en) * 2015-01-04 2015-04-29 北京世纪合辉医药科技股份有限公司 Health food for improving bone and joint health as well as preparation method thereof
CN105661509A (en) * 2016-01-18 2016-06-15 烟台新时代健康产业有限公司 Krill oil softgel for protecting bone health as well as preparation method and application thereof
CN108719970A (en) * 2018-04-24 2018-11-02 海南久惠生物科技有限公司 A kind of composition, health products and preparation method thereof comprising it increasing bone density
CN114634901A (en) * 2022-05-18 2022-06-17 微康益生菌(苏州)股份有限公司 Lactobacillus casei LC16 for promoting bone health and culture method and application thereof
CN115804759A (en) * 2022-12-09 2023-03-17 江苏扬新生物医药有限公司 A granule containing coenzyme Q10 and calcium

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CN104273541A (en) * 2014-10-29 2015-01-14 成都健怡营销策划有限公司 Health product for increasing bone mineral density
CN104543674A (en) * 2015-01-04 2015-04-29 北京世纪合辉医药科技股份有限公司 Health food for improving bone and joint health as well as preparation method thereof
CN105661509A (en) * 2016-01-18 2016-06-15 烟台新时代健康产业有限公司 Krill oil softgel for protecting bone health as well as preparation method and application thereof
CN108719970A (en) * 2018-04-24 2018-11-02 海南久惠生物科技有限公司 A kind of composition, health products and preparation method thereof comprising it increasing bone density
CN114634901A (en) * 2022-05-18 2022-06-17 微康益生菌(苏州)股份有限公司 Lactobacillus casei LC16 for promoting bone health and culture method and application thereof
CN114634901B (en) * 2022-05-18 2022-08-30 微康益生菌(苏州)股份有限公司 Lactobacillus casei LC16 for promoting bone health and culture method and application thereof
CN115804759A (en) * 2022-12-09 2023-03-17 江苏扬新生物医药有限公司 A granule containing coenzyme Q10 and calcium
CN115804759B (en) * 2022-12-09 2024-05-10 江苏扬新生物医药有限公司 Granule containing coenzyme Q10 and calcium

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