KR20100056329A - Calcium compound and calcium compound fertilized rice for the prevention and treatment of osteoporosis - Google Patents

Abstract

PURPOSE: A calcium compound for preventing and treating osteoporosis and a calcium compound fertilized rice are provided to improve calcium absorption and to enhance blood osteocalcin. CONSTITUTION: A composition for preventing and treating osteoporosis contains ionized calcium as an active ingredient. The ionized calcium is shell ionized calcium. A method for producing the ionized calcium comprises: a step of pulverizing shell in a size of 1.5-10cm; a step of loading shell in a plasticizing path; a step of plasticizing by 1200°C; a step of blocking heat to maintain enhanced temperature inside the plasticizing path; a step of gradually cooling by 200-400°C; a step of cooling by 100-150°C; and a step of taking out the shell and cooling by room temperature.

Description

Calcium Compound and Calcium Compound Fertilized Rice for the Prevention and Treatment of Osteoporosis}

The present invention relates to calcium compounds and calcium compound fertilized rice for the prevention and treatment of osteoporosis, and specifically to the use of shell ionized calcium having excellent calcium absorption in vivo, or for use in the prevention and treatment of osteoporosis using rice cultivated using the same as fertilizer. It is about.

As the economy grows and our diet becomes more abundant, our meat intake increases and our daily use of instant foods becomes more busy, so our bodies gradually become acidic and indispensable to our bodies. Lack of calcium leads to poor skeletal growth in adolescents, women in pregnant and lactating women, discord of body rhythms, and fatal osteoporosis in postmenopausal women. Many kinds of calcium supplements are currently on the market for supplementation of the insufficient calcium, but most of them are not completely absorbed in the body, and there is a problem in that the content does not reach an appropriate level.

Calcium is mainly contained in bone or teeth in combination with phosphoric acid in the body, and is also involved in important physiological functions. Especially, calcium reduces the incidence of chronic diseases such as osteoporosis and hypercholesterolemia, arteriosclerosis, hyperlipidemia or hypertension. It plays an important role in ensuring that proper calcium nutrition is maintained at all times. The daily recommended amount of calcium is about 0.8 g. Intake of calcium through food is very important, but in fact, the absorption of calcium in the body is very low even when eating foods containing calcium. Only up to 45% of the situation is ingested. In order for calcium to be absorbed into the body, it must be present in soluble form when passing through mucosal cells in the small intestine, which is the site where calcium is absorbed. Will be lowered.

Calcium is the most abundant mineral in the human body. The average adult has about 1,200 g of body weight, which is about 2% of the body weight. 99% of the body's calcium forms skeleton and teeth. Only about 1% is responsible for regulating physiological activities such as muscle contraction and relaxation, regular heartbeat, blood coagulation, enzyme activation, intracellular stimulation and excitability delivery (Einhorn, TA, et al., Nutrition and Bone.Ortho.Clin.Nor.Am . , 21, 43-50, 1990; Allen, LH, et al., In modern Nutrition in Health and Diseases. 8th.ed., 144-163, 1994). In addition, its role as a regulator of cell membrane mass transfer and a second messenger in cells has long been important. Most of the calcium in the body (99%) is present in the skeleton and teeth, and only a few are present in cells and fluids inside and outside the cells to perform the body's physiological control functions.

Ingested calcium is mostly solubilized in the stomach and moved to the small intestine, mainly in the upper small intestine is absorbed by active transport (passive diffusion) in the lower small intestine. Absorption efficiency is relatively low and may be promoted or inhibited by the body's physiology, body demand, intake and various substances present in the small intestine, and may also be affected by the various dietary ingredients taken together. In general adults, the apparent absorption rate is in the range of 10 to 40% and does not exceed 50 to 60% even if the body demand is high. The concentration of calcium in serum, which is responsible for various body control functions, is always maintained around 10 mg / dL (Frances, JZ, et al., Application of Clin. Nutrition, Prentice Hall, Englewood Cliffs, New Jersey). , 1988).

Calcium escapes, osteoporosis, rickets. Deficiencies such as tetany are induced (Allen, LH, et al., In modern Nutrition in Health and Diseases . 8th.ed., 144-163, 1994; Scalmati A., et al., Clinics in Applied Nutrition , Andover Med. , 67-74, 1992; McCarren, DA, et al., Am. J. Clin. Nutr. , 65, 712-716, 1997), resulting in bones with low calcium concentrations that tend to fracture ( Chung, et al., J. Korean Soc.Food.Sci.Nutr. , 28, 677-684, 1999).

 In recent years, many countries around the world focus attention on many nutritional problems such as bone disease, osteoporosis, fracture, circulatory disease, hypertension, arteriosclerosis or hyperlipidemia, as well as bone growth and maintenance of nutritional problems due to lack of calcium intake. In particular, the importance of calcium intake is emphasized in maintaining the health of the skeleton and the prevention and treatment of skeletal diseases caused by aging.

As a natural food, calcium absorption through calcium salt is reported to be absorbed only about 50% of the calcium contained in milk, 25% for anchovies and shellfish, 20% for vegetables. It is known to be absorbed. This is due to the fact that calcium that can be absorbed in the body is limited to a substance having a calcium oligopeptide structure that sustains physiological activity.

On the other hand, calcium from food is absorbed by the intestine, but its absorption rate is relatively low, and it is influenced by other forms of calcium such as calcium presence, intake and vitamins (Allen, LH, et al., In modern Nutrition in Health). and Diseases . 8th.ed., 144-163, 1994). The absorption rate of calcium in the diet of Korea is reported to be 12 ~ 46%, and it is generally known that about 20 to 80% of the intake is excreted in feces. Recently, the daily intake of calcium by adults in Korea is 511 mg, which is less than the recommended daily dose of 70 mg, so the interest and consumption of calcium supplements is gradually increasing (Ministry of Health and Welfare, National Nutrition Survey Report, 1998) And 2000).

In general, calcium is one of the essential nutrients essential to the formation of bones and teeth, a lack of calcium intake may cause rickets, and in severe cases may deform the bone shape. In addition, calcium is an important factor involved in vital activities, such as bone constituents in addition to the components that form bone, the movement of substances through the cell membrane, hormones, digestive fluid secretion, blood coagulation of the wound.

In recent years, various efforts have been made to reinforce calcium in foods to alleviate the shortage of calcium intake. In particular, more calcium is added to milk, which is known to contain relatively large amounts of calcium, and other juices and milk powders. Foods such as calcium is added and sold.

As described above, the method of adding calcium to food has been invented for a long time. The method of adding calcium to food, particularly beverages, is added by using water-soluble calcium preparations such as calcium lactate or calcium chloride, and calcium carbonate and calcium phosphate. Or it can classify by the method of adding using water-insoluble calcium preparations, such as eggshell calcium. However, when water-soluble calcium, such as calcium lactate or calcium chloride, is added to milk or soy milk, when calcium is added at 0.02% or more, calcium ions and proteins react during the heat sterilization process, causing precipitation of the protein and poor flavor. There is a limit to the addition can adversely affect the original taste.

Accordingly, recently, methods of atomizing calcium carbonate or calcium phosphate, which are water-insoluble calcium, and dispersing them in foods by adding a dispersant have been invented. As an example, as a method of adding calcium carbonate, crystal cellulose is added to milk together with calcium carbonate to maintain calcium carbonate in the network structure of cellulose. In this case, however, as the crystalline cellulose is excessively added to the milk, the original viscosity of the milk is increased, and the flavor is not good, which is not suitable for use in milk and soy milk.

On the other hand, in order to use calcium carbonate in water-insoluble calcium as a food additive, synthetic calcium carbonate obtained by dehydrating and drying the slurry of calcium carbonate obtained by injecting carbonic acid gas into an aqueous suspension of 5-30% calcium hydroxide (lime oil) is used. do. However, the average particle size of the calcium carbonate slurry produced by such a reaction process is 0.04 ~ 1 ㎛, the average particle size is 1 ~ 10 ㎛ because the particles of calcium carbonate agglomerated again during the dehydration and drying process to powder It becomes difficult to use in food.

In order to solve this problem, Japanese Patent Application Laid-Open No. 64-69513 adds sucrose fatty acid ester, which is a hydrophilic emulsifier of HLB 10 or more, to a 10% calcium carbonate slurry without going through a powdering process in the manufacturing process of calcium carbonate, and then ultrasonically A method of improving water dispersibility by atomizing calcium carbonate by treating with has been proposed. However, using this method, it is possible to produce a low concentration calcium carbonate slurry but not to produce a high concentration calcium carbonate slurry, so that an excess calcium carbonate slurry should be added to the food. The disadvantage is that it takes.

In addition, Korean Patent No. 123539 and Japanese Patent Laid-Open Nos. Hei 6-56423 and 7-138018 disclose that 10 to 20% of calcium carbonate is wet-pulverized to add a certain amount of sucrose fatty acid ester, which is a hydrophilic emulsifier of HLB 10 or more, to a solid content of calcium carbonate. How to do it was introduced. However, in this method, when 0.5% or more of sucrose fatty acid ester is added in water, the gelation occurs severely, and the viscosity of the calcium carbonate slurry rapidly rises, resulting in very poor water dispersibility and low calcium carbonate concentration. It takes a lot of distribution cost in the slurry, there is a disadvantage that the amount of the input into the beverage may have a bad effect on the flavor of the product. In addition, when the powder is manufactured and powdered in this manner, there is an inconvenience in that the powdered product is not easily dissolved in water, and thus, the beverage should be added after the beverage is warmed.

In addition, Japanese Patent Application Laid-open No. Hei 10-70966 proposes a method of using a dispersant by grinding calcium carbonate and mixing a sucrose fatty acid ester with an anionic surfactant. In this case, however, the viscosity of the dispersant is lowered, but there is a problem that the unique taste of the organic acid affects the food by adding an anionic organic fatty acid ester as the dispersant, which is undesirable.

In addition, Korean Patent Nos. 0491425 and 0492656, filed and registered by Japanese Maruo Calcium Co., Ltd., add Arabic gum or arabinogalactan as a stabilizer to improve water dispersibility and stability due to the addition of such water-insoluble calcium agent. It can be said. However, even with these stabilizers, sufficient water dispersibility and stability have not been obtained. Therefore, continuous development and review is required.

Recently, the average life expectancy of humans has been prolonged with the advent of an aging society, but patients suffering from diseases such as osteoporosis, rheumatism or degenerative arthritis, which are called lifestyle diseases, are increasing rapidly. In addition, it is pointed out that the lack of calcium intake is remarkable in the growing children and the elderly, and to solve this problem, water-soluble inorganic or organic acid calcium, calcium carbonate, calcium phosphate, eggshell calcium, right bone calcium, etc. Various calcium fortified foods are added to the market with the addition of inorganic calcium, which is insoluble in water, such as whey calcium, coral calcium and seaweed calcium. However, water-soluble organic calcium carbonate has a phenomenon that the absorption is lowered compared to the intake due to side effects such as free calcium ions combine with the protein in the food to form a network structure and precipitate, thereby inhibiting the stability of the protein. In particular, in the case of milk protein, most of the calcium is present in the combined state with casein, which is formed by the macromolecules, the absorption amount is dropped due to the formation of macromolecules are being sought in various ways.

To date, various inventions have been developed to promote absorption of calcium. A method comprising treating calcium carbonate with a hydrophilic emulsifier to safely suspend calcium insoluble in water (Japanese Patent Publication No. Hei 2-31942), stirring a water-soluble calcium salt to obtain an average particle size of 1 μm. After preparing a calcium salt having a method comprising the addition of a hydrophilic emulsifier (Japanese Patent Laid-Open No. Hei 8-107772), by treating a mixture of calcium carbonate and emulsifier with a high pressure homogenizer and then dispersing it ( Japanese Patent Application Laid-Open No. Hei 8-205820) and the like, but all of these inventions are improved methods of changing the form of dispersion or solubilizing, and are not intended to be directly involved in calcium absorption.

In addition, a method of promoting calcium absorption in a form that supports calcium well in a product containing calcium (Japanese Patent Publication No. 56-117753) and a method of improving dispersibility of calcium carbonate by irradiating with ultrasonic waves (Japan Patent Application Publication No. 64-69513) and the like, but these inventions are not a method of using a medium that promotes absorption of calcium. In other words, it would not be an exaggeration to say that the focus is on improving the emulsion state of the solution containing calcium, improving the dispersion method, or stabilizing the dispersion.

In addition, in Korea, at least 1 in the group consisting of sucrose fatty acid ester having 10 HLB (Hydrophilic Lipophlic Balance) or calcium carbonate mixture (Reg. No. 10-123539), calcium carbonate, calcium phosphate and ferric pyrophosphate Wet mixtures in which Arabic gum and a lipophilic emulsifier were added to at least one of the group consisting of propylene glycol ester (PGA) added to the type (Reg. No. 10-262379), calcium carbonate, calcium phosphate and ferric pyrophosphate. The method of preparing calcium carbonate dispersion by wet milling with a grinder (see Korean Patent No. 2000-76185) is also a method of stabilizing calcium by changing the properties of the calcium mixture, and thus, there is a limit to the direct absorption promoting activity. can do.

In addition, as a method for promoting calcium absorption, there is a method of using a low molecular weight phosphorylated chitosan oligosaccharide (Patent No. 2002-93721) and a method of preparing high-efficiency foods with improved calcium absorption (Public Patent No. 2002-19992), but oligosaccharides or oligos It can be said to improve calcium absorption by maximizing peptide production. These can all be said to utilize the power of oligosaccharides produced through methods such as acid treatment or enzymatic digestion. Similarly, there is a composition containing easily absorbable calcium and a method for preparing the same (Public Patent No. 1996-40195), which includes chondroitin sulfate, which can also be said to utilize the binding force of oligosaccharides.

As described above, most of the conventional techniques are related to stabilization of calcium solution or increase of absorption through it, and adsorption of calcium using hydroxyl groups of various oligosaccharides and increased absorption using the same.

These stabilization of the calcium solution is very different depending on the emulsion state, there is a limit to increase the actual absorption of calcium because the stability may be greatly reduced by gastric acid and the like. In addition, the method using oligosaccharides has various problems such as the question of whether a stable binding force can be exhibited and the instability of oligosaccharides by various degrading enzymes, the stability due to strong acid added during the production of oligosaccharides, and the formation of an unpleasant taste.

Therefore, the inventors of the present invention, while studying the method of treating osteoporosis using a compound that promotes the absorption of calcium in vivo, animals orally administered shell-ionized calcium or rice cultivated using the same as fertilizer orally administered a general diet or general rice By increasing the concentration of calcium, osteocalcin and parathyroid hormone (PTH) in the blood and increasing the concentration of calcium and phosphorus in the femur compared to animals, the findings can be used to prevent and treat osteoporosis. The present invention has been completed.

An object of the present invention is to provide a composition for the prevention and treatment of osteoporosis containing calcium ion, the rice wine functional food for preventing and improving osteoporosis, and the rice for preventing and improving osteoporosis cultivated by fertilizing the calcium ion in rice.

In order to provide the above object, the present invention provides a composition for the prevention and treatment of osteoporosis containing calcium ion as an active ingredient.

The present invention also provides a method for preventing or treating osteoporosis, comprising administering to a subject a pharmaceutically effective amount of the composition.

The present invention also provides a health functional food for preventing and improving osteoporosis containing calcium ion as an active ingredient.

In addition, the present invention provides a rice having an osteoporosis prevention and improvement effect prepared by fertilizing calcium ionized rice.

In addition, the present invention provides a method for preventing or improving osteoporosis, comprising orally administering a rice having an osteoporosis prevention and improvement effect prepared by fertilizing calcium ion to rice.

In another aspect, the present invention provides a method for producing rice having an effect of preventing and improving osteoporosis, comprising the step of fertilizing the calcium ionized powder as fertilizer in rice.

In addition, the present invention provides a dietary supplement for osteoporosis prevention and improvement containing rice as an active ingredient, which has the effect of preventing and improving osteoporosis prepared by fertilizing calcium ionized with fertilizer to rice.

The calcium compound and calcium compound fertilized rice of the present invention increases the concentration of calcium in the blood, increases the concentration of osteocalcin (osteocalcin), which is an indicator of bone formation, and reduces the concentration of parathyroid hormone (PTH), a calcium regulatory hormone, By increasing the concentration of calcium and phosphorus in the femur, it can be usefully used as a composition for preventing and treating osteoporosis, or as a dietary supplement for preventing and improving osteoporosis.

Hereinafter, terms used in the present invention are defined.

As used herein, the term "prevention" means any action that inhibits the formation or delays the progression of osteoporosis by administration of the composition of the present invention.

As used herein, the terms "treatment" and "improvement" refer to any action in which the symptoms of osteoporosis improve or benefit altered by administration of the composition of the present invention.

As used herein, the term "administration" means providing a subject with any of the compositions of the present invention in any suitable manner.

As used herein, the term "individual" means any animal, such as a human, monkey, dog, goat, pig or rat, having a disease in which the symptoms of osteoporosis can be improved by administering the composition of the present invention.

As used herein, the term “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit or risk ratio applicable to medical treatment, which means the type of disease, the severity, the activity of the drug, the drug Sensitivity to, time of administration, route of administration and rate of administration, duration of treatment, factors including drug used concurrently, and other factors well known in the medical arts.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for the prevention and treatment of osteoporosis containing calcium ion as an active ingredient.

The calcium ion is preferably prepared by a method consisting of the following steps, but is not limited thereto:

1) crushing the shell to a size of 1.5 to 10 cm;

2) after the crushed shell is loaded into the firing furnace, the temperature is gradually raised to 1200 ° C. to fire the shell;

3) The heat supply for heating of the kiln is cut off, and the cooled shell is gradually cooled while keeping the heated shell inside the kiln, until the temperature inside the kiln is 200 to 400 ° C, and then the door of the kiln is gradually opened to the kiln. Cooling the furnace while gradually entering the outside air until the internal temperature of the kiln reaches 100 to 150 ° C, and then taking the shell out of the kiln and cooling it in the air until it reaches room temperature (see Korean Patent No. 10-0707737). ).

The ionized calcium of the present invention is preferably shell ionized calcium extracted from pearl, coral, scallops shell, but is not limited thereto.

Calcium ionization of the present invention is preferably prepared to facilitate absorption in vivo by ionizing calcium in order to provide high purity calcium to the human body and agricultural and livestock products under various purposes, such as edible, medical or agricultural livestock administration. Do not.

Since the calcium ionization of the present invention has a high degree of ionization, it is preferable that calcium absorption is much higher for the donor, but is not limited thereto.

The calcium ionization of the present invention is preferably a calcium compound that naturally contains beneficial elements such as calcium as well as various essential minerals, but is not limited thereto.

The calcium ionization of the present invention has high biocalcium absorption, and thus can be usefully used for the prevention and treatment of osteoporosis.

In the present invention, the shell ionized calcium prepared according to the above method is diluted in distilled water, prepared in emulsion form, and then orally administered to the rat, and blood is collected to calculate calcium concentration, blood phosphorus (P) concentration, and blood osteocalcin. Osteocalcin concentration and parathyroid hormone (PTH) concentration were measured, respectively. As a result, in the shell-ionized calcium-administered group of the present invention, the blood calcium concentration was increased by about 4 to 6%, the blood phosphorus concentration was reduced by about 10% to 16%, and the blood osteocalcin concentration was increased by about 25 to 27% compared to the control group. Parathyroid hormone levels in blood decreased by about 16 to 18%.

Calcium (Ca) is present in the body of about 1kg of which 99.9% is in the bone, 0.1% is in equilibrium in the extracellular fluid. Calcium in the extracellular fluid is in constant balance with the Ca pool of bone. In other words, the blood calcium concentration is generally kept constant. Therefore, it can be seen that the shell ionized calcium of the present invention is very effective for absorption of calcium in vivo.

Osteocalcin is a substance that is tightly bound to hydroxyapatite (HAp) and calcium in the structure of bone and is an bone formation index that indirectly reflects the activity of osteoblasts in forming new bone. It is a diagnostic marker of osteoporosis and an increase in the level indicates a small risk of osteoporosis. In addition, osteocalcin is a protein produced only in bone and promoted synthesis by vitamin D, and its specificity is high, which is highly valuable for evaluating the activity of osteoblasts and examining the degree of bone formation. In other words, the concentration of osteocalcin in the blood has a significant correlation with bone growth, and its increase means an increase in bone formation due to increased activity of osteoblasts. Therefore, it can be seen that the shell ionized calcium of the present invention is very effective for osteoporosis.

Parathyroid hormone (PTH) is a hormone that maintains the homeostasis of serum calcium levels. The calcium level is high in primary hyperthyroidism and low in primary parathyroidism. In addition, in order to maintain homeostasis against external factors, the concentration of parathyroid hormone acts to normalize calcium concentration, so it rises for hypocalcemia and decreases for hypercalcemia. Therefore, it can be seen that the shell ionized calcium of the present invention is very effective for absorption of calcium in vivo.

In addition, in the present invention, the ionized calcium prepared according to the above method is diluted in distilled water, prepared in an emulsion form, and orally administered to the rat, followed by dissecting the femur of the rat to remove the adherents such as muscle, and then, in hydrochloric acid and nitric acid solution. After dissolution, the concentrations of calcium and phosphorus in the skeleton were measured, respectively. As a result, the concentration of calcium in the skeleton was increased about 30 to 31%, the concentration of phosphorus in the skeleton was increased by about 15% to 16% compared to the control group. Therefore, it can be seen that the calcium ionization of the present invention is very effective in the absorption of calcium in vivo, reducing the risk of osteoporosis.

When using the calcium ionization of this invention as a pharmaceutical, it may further contain 1 or more types of active ingredients which show the same or similar function.

The calcium ion can be administered orally or parenterally during clinical administration and can be used in the form of a general pharmaceutical formulation.

In other words, the calcium ionization of the present invention may be administered in various oral and parenteral dosage forms in actual clinical administration, and when formulated, diluents such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. that are commonly used, or Formulated using excipients. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, Calcium carbonate, Sucrose (Lucose) or lactose (Lactose), gelatin and the like are mixed and prepared. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The dosage of the calcium ionization of the present invention varies depending on the body weight, age, sex, health condition, diet, time of administration, administration method, excretion rate and severity of the disease, the daily dosage of the shell ionized calcium The amount is 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg, and may be administered 1 to 6 times a day.

The calcium ionization of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers.

The present invention also provides a method for preventing or treating osteoporosis, comprising administering to a subject a pharmaceutically effective amount of the composition.

The composition may contain one or more active ingredients exhibiting the same or similar functions in addition to the calcium ionization of the present invention.

The composition may be administered orally or parenterally during clinical administration and may be used in the form of a general pharmaceutical formulation.

The dosage of the composition varies depending on the weight, age, sex, health condition, diet, time of administration, method of administration, excretion rate and severity of the disease of the patient, the daily dosage is based on the amount of shell ionized calcium 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg, and may be administered 1 to 6 times a day.

The method can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers.

The present invention also provides a health functional food for preventing and improving osteoporosis containing calcium ion as an active ingredient.

The ionized calcium is preferably shell ionized calcium extracted from pearls, corals, scallops shells, but is not limited thereto.

The ionized calcium is preferably prepared to facilitate absorption in vivo by ionizing calcium in order to provide high purity calcium to the human body and agricultural and livestock products under various purposes such as edible, medical or agricultural livestock administration.

The ionized calcium has a high degree of ionization, it is preferable that the calcium absorption rate is significantly higher for the donor, but is not limited thereto.

The calcium ion is excellent in the absorption of calcium in vivo to increase the blood calcium concentration, increase the concentration of osteocalcin, a bone formation marker, decrease the concentration of parathyroid hormone, a calcium regulatory hormone, calcium and phosphorus concentration in the femur By increasing it can be usefully used as an active ingredient in health functional foods for the prevention and improvement of osteoporosis.

In the case of using the calcium ion as a food additive, the shell calcium ion may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). In general, the shell calcium ion of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the total raw materials in the manufacture of food or beverage. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and includes all health foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid. Carbonating agents and the like used in beverages. In addition, the composition of the present invention may contain a pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a rice having an osteoporosis prevention and improvement effect prepared by fertilizing calcium ionized rice, and a method for producing the osteoporosis prevention and improvement rice.

The ionized calcium is preferably shell ionized calcium extracted from pearls, corals, scallops shells, but is not limited thereto.

The calcium ion is preferably manufactured by a known method (see Korean Patent No. 10-0707737), but is not limited thereto.

Osteoporosis prevention and improvement of the rice of the present invention is preferably prepared by a method comprising the step of cultivating the calcium ionized powder as a fertilizer, but is not limited thereto.

In the cultivation of the rice for preventing and improving osteoporosis of the present invention, it is preferable to apply the calcium ionized powder in the fertilizer form three times from 4 weeks after planting rice to just before sprouting, and three times from harvesting until harvesting, but not limited thereto. .

Osteoporosis prevention and improvement of the rice of the present invention can be usefully used for the prevention and improvement of osteoporosis by having a high biological calcium absorption.

Rice cultivated by administering the calcium compound of the present invention in the form of fertilizers also has a higher calcium content (300 mg / Kg, ordinary rice 45 mg / Kg) than ordinary rice, and thus has high biocalcium absorption compared to ordinary rice. Therapeutic efficacy.

In the present invention, the rice prepared according to the above method was orally administered to rats, and blood of the rats was collected to measure concentrations of calcium, phosphorus, osteocalcin and parathyroid hormone in the blood, respectively. As a result, in the rice administration group of the present invention, the blood calcium concentration was increased by about 4 to 6%, the blood phosphorus concentration was reduced by about 10% to 16%, and the blood osteocalcin concentration was increased by about 25 to 27%, compared to the control group. Parathyroid hormone levels in blood decreased by about 16 to 18%.

In addition, in the present invention, the rice prepared according to the above method is orally administered to the rat, and then dissecting the femur of the rat to remove the adherents, such as muscle, and then dissolved in a hydrochloric acid and nitric acid solution to the concentration of calcium and phosphorus in the skeleton, respectively Measured. As a result, the concentration of calcium in the skeleton was increased about 30 ~ 31%, the concentration of phosphorus in the skeleton increased about 15% ~ 16% compared to the control group of the rice administration group of the present invention.

Therefore, the rice of the present invention is excellent in the absorption of calcium in vivo to increase the blood calcium concentration, increase the concentration of osteocalcin, a bone formation marker, decrease the concentration of parathyroid hormone, a calcium regulatory hormone, calcium and phosphorus in the femur By increasing the concentration can be usefully used as a functional rice for the prevention and improvement of osteoporosis.

In addition, the present invention provides a method for preventing or improving osteoporosis, comprising orally administering a rice having an osteoporosis prevention and improvement effect prepared by fertilizing calcium ion to rice.

Osteoporosis prevention or improvement of the rice of the present invention can be used in conjunction with the rice alone as well as other food or food ingredients when administered orally, can be suitably used according to a conventional intake method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). Even in the case of long-term intake for health and hygiene purposes or health control purposes, the rice is not limited to a specific range of amounts because there is no problem in terms of safety.

In addition, the present invention provides a dietary supplement for osteoporosis prevention and improvement containing rice as an active ingredient having a prevention and improvement effect of osteoporosis prepared by fertilizing calcium ion in rice.

The ionized calcium is preferably shell ionized calcium extracted from pearls, corals, scallops shells, but is not limited thereto.

The calcium ion is preferably manufactured by a known method (see Korean Patent No. 10-0707737), but is not limited thereto.

Rice of the present invention is excellent in the absorption of calcium in vivo to increase blood calcium concentration, increase the concentration of osteocalcin, a bone formation marker, decrease the concentration of parathyroid hormone, a calcium regulatory hormone, calcium and phosphorus concentration in the femur By increasing the can be usefully used as an active ingredient in health functional foods for the prevention and improvement of osteoporosis.

Osteoporosis prevention and improvement of the rice of the present invention is preferably prepared by a method comprising the step of cultivating the calcium ionized powder as a fertilizer, but is not limited thereto.

In the cultivation of the rice for preventing and improving osteoporosis of the present invention, it is preferable to apply the shell ionized powder in fertilizer form three times from 4 weeks after planting rice to just before sprouting, and three times from harvesting until harvesting, but not limited thereto. Do not.

When the rice of the present invention is used as a food additive, the rice may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). In general, the rice of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the total raw material in the production of food or beverage. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid. Carbonating agents and the like used in beverages. In addition, the composition of the present invention may contain a pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Preparation Examples.

However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples, Experimental Examples, and Preparation Examples.

Example 1 Preparation of Calcium Compound Formulations

100 g of shell ionized calcium (Nucarion, Korea) prepared according to the manufacturing method described in Korean Patent No. 10-0707737 was diluted to 10% concentration in distilled water to give a total volume of 1000 ml. Prepared in the form.

Example 2 Preparation of Calcium Compound Fertilized Rice Formulation

The calcium compound powder prepared in <Example 1> was administered in the form of fertilizer, and then the cultivated rice was pulverized. After mixing to form a pellet (pellet) using 20 kg of water to prepare a formulation dried in the oven.

<Example 3> Calcium compound and calcium compound fertilized rice administration animal experiment

<3-1> Preparation of Experiment Animal

Fifty male Sprague-Dawley rats (central laboratory animals) weighing 270-300 g at 8 weeks of age were subjected to temperature 21 ± 3 ° C, relative humidity 50 ± 5%, and roughness 200 to 300 lux for 7 days. After adapting to the laboratory of conditions, only 25 healthy animals closest to the standard body weight were selected and used for testing. During the adjustment period, tap water was freely consumed as drinking water and experimental animal feed was freely used as feed. The composition of the experimental animal feed was shown in Table 1 below.

turn nutrient Content rate (%) One Crude protein 22.1 or later 2 Low fat 3.5 or more 3 fibrin 5.0 or less 4 Ash 8.0 or less 5 calcium 0.6 or more 6 sign 1.2 or less

<3-2> Distribution of Experimental Group

25 animals selected in Example <3-1> were randomly divided into 5 groups of 5 animals and used for the test. Specifically, as shown in Table 2 below, the first group was fed a general rice and a super feed mixed feed (general rice: super feed feed = 3: 1) for 8 weeks as a negative control. Group 2, 3, 4 and 5 are the test group, the second group is a calcium fertilized rice and super feed mixed feed prepared in Example 2 (calcium fertilized rice: super feed feed = 3 : 1) was fed. In the third group, the calcium compound preparation prepared in Example 1 was diluted in distilled water by 10% by weight while administering a general rice and a super feed mixed feed (general rice: super feed feed = 3: 1) as feed. Rats were orally administered in the form of 1 ml / Kg at 8-week intervals once daily. The fourth group is the calcium prepared in <Example 1> while administering the calcium fertilized rice and super feed mixed feed (calcium fertilized rice: super feed feed = 3: 1) prepared in <Example 2> as a feed Compound formulations were diluted in distilled water at a 10% weight ratio and administered orally to rats in emulsion form at a 1 ml / Kg dose once daily for 8 weeks.

Experimental group feed Oral administration First group Normal rice: Superfeed feed = 3: 1 Distilled water 2nd group Calcium Fertilized Rice: Superfeed Feed = 3: 1 Distilled water 3rd group Normal rice: Superfeed feed = 3: 1 Calcium Compound Formulation 4th group Calcium Fertilized Rice: Superfeed Feed = 3: 1 Calcium Compound Formulation

<3-3> Sampling

After oral administration of feed and calcium preparations as in Example <3-2>, each experimental group was fasted for 12 hours and sacrificed after 8 weeks, and the heart was directly inserted into the heart (heart pucture). Blood was collected from. Samples were immediately rotated at 3,000 rpm in a centrifuge and only serum was collected and sent to the medical center to measure various test items. In addition, the skeleton dissected the femur of the rat to remove the adherents such as muscle, and then immediately sent to the analytical research center to dissolve in hydrochloric acid and nitric acid solution.

Experimental Example 1 Measurement of Blood Calcium Concentration

The present inventors measured the calcium concentration in blood using the o-cresol phenolphtalein complexone (OCPC) end point colorimetric method from the blood sampled in the above <3-3>. Specifically, calcium ions combine with the complex of o-cresolphthalein to form a purple complex in basic culture, and calcium (Ca) is combined with the o-cresolphenolphthalein complex and alkaline. The reddish violet complex salt is formed under the conditions. At this time, the degree of calcium was determined by measuring the absorbance of the reddish violet color. The conditions used for the experiments were as follows: Reference values were 8.2-10.8 mg / dL (human serum), reagent used was Ca (Simens, U.S.A), and the measuring instrument was ADVIA 1650 (Simens, U.S.A).

Experimental group Calcium in blood (mg / dl) (average value for each group) First group 10.05 2nd group 10.60 3rd group 10.50 4th group 10.80

As a result, as shown in Table 3, the blood calcium concentration was increased by about 4.5% to 5.5% in the calcium fertilized rice diet and the calcium oral administration group compared to the general US diet group, and the fourth group showed synergistic effects. Is shown (Table 3). Therefore, the blood calcium concentration remains almost constant, but it can be seen that the calcium fertilized rice diet and oral administration of calcium preparations are very effective for calcium absorption.

Experimental Example 2 Measurement of Phosphorus Concentration in Blood

The present inventors measured the concentration of phosphorus in blood by using the Molybdate-UV colorimetric method from the blood sampled in the above <3-3>. Specifically, since inorganic phosphorus reacted with ammonium molybdate in the presence of sulfuric acid to form a phosphomolybdate complex, the concentration was determined by measuring at 340 nm. The conditions used in the experiment were as follows: Reference values were 2.5 to 5.5 mg / dL (human serum), reagent used was phosphorus (Simens, U.S.A), and the measuring instrument was ADVIA 1650 (Simens, U.S.A).

Experimental group Blood phosphorus (mg / dl) (average value for each group) First group 8.25 2nd group 7.50 3rd group 6.95 4th group 6.85

As a result, as shown in Table 4, the blood phosphorus concentration was reduced by about 10% to 16% in the calcium fertilized rice diet and the calcium oral administration group compared to the general diet group, and the fourth group showed synergistic effects. Is shown (Table 4). It can be seen that the calcium fertilized rice diet and oral administration of calcium preparations are very effective for calcium absorption.

Experimental Example 3 Measurement of Osteocalcin Concentration in Blood

The present inventors measured the concentration of osteocalcin in the blood using ECLIA (Electrochemiluminescent Immunoassay) from the blood sampled in Example <3-3>. Specifically, quantification of osteocalcin was measured using electrochemiluninescence (ECLA) in serum or plasma using E-170 osteocalcin analysis. The conditions used in the experiments were as follows: Reference values are 24 to 70 ng / mL (human serum) between 18 and 29 years old for men, 14 to 42 ng / mL for 30 and 49 years old, and 50 to 70 years. 14 to 46 ng / mL at age, 20 to 11 ng / mL for premenopausal women, 15 to 46 ng / mL for postmenopausal (No HRT), and premenopausal women (> 20 years). 11 to 43 ng / ml for postmenopausal women and 15 to 46 ng / ml for postmenopausal women (without hormone replacement, no HRT), and the reagent used is Osteocalcin (Roche, Switzerland). E-modular 170 (Roche, Switzerland).

Experimental group Osteocalcin (ng / ml) (average value for each group) First group 57.075 2nd group 70.075 3rd group 72.075 4th group 73.075

As a result, as shown in Table 5, the calcium fertilized rice administration group (Group 2) increased the osteocalcin concentration by about 22.8% compared to the general US administration group (Group 1). Osteocalcin concentration was increased by about 26.3% compared to group 1), and the group administered with oral administration of calcium fertilized rice and calcium preparation (Group 4) also increased about 28.0% compared to the general untreated group (Group 1) (Table 5). This reflects a reduced state of osteoporosis risk in the experimental group, it can be seen that due to the high calcium absorption of calcium fertilized rice and calcium preparations.

Experimental Example 4 Measurement of Parathyroid Hormone (PTH) Level in Blood

The present inventors measured the concentration of parathyroid hormone in the blood by using ECLIA (Electrochemiluminescent Immunoassay) from the blood sampled in Example <3-3>. Specifically, the quantification of PTH was measured using electrochemiluninescence (ECLA) in serum or plasma using Elecsys PTH analysis. The conditions used in the experiment were as follows: Reference value was 15-65 pg / mL (human serum), reagent used was PTH reagent (Roche, Switzerland), and the measuring instrument was E-Modular 170 (Roche, Switzerland).

Experimental group PTH (pg / ml) (average value for each group) First group 2.32 2nd group 2.045 3rd group 1.925 4th group 1.830

As a result, as shown in Table 6, the calcium fertilized rice administration group (Group 2) had a PTH concentration of about 12% less than that of the general US administration group (Group 1). The PTH concentration was reduced by about 17% compared to the first group, and the group that received oral administration of calcium fertilized rice and calcium preparation (Group 4) also reduced the synergy effect by about 21% compared to the general untreated group (Group 1). Is shown (Table 6). This reflects the high calcium state of the experimental group, it can be seen that due to the high calcium absorption of calcium fertilized rice and calcium preparations.

Experimental Example 5 Measurement of Calcium and Phosphorus Concentrations in the Femur

The present inventors use ICP (Inductively Coupled Plasma) (ICP-MS SYSTEM, PerkinElmer) to calculate the concentration of calcium and phosphorus in the skeleton by dissolving the skeleton obtained in Example <3-3> in hydrochloric acid and nitric acid solution. It was measured by.

Experimental group Calcium (mg / g) (average value for each group) Phosphorus (mg / g) (average value for each group) First group 95.1 42.5 2nd group 124.5 49.2 3rd group 149.5 54.0 4th group 151.5 55.5

As a result, as shown in Table 7, the calcium fertilized rice administration group (Group 2) increased calcium and phosphorus concentrations by about 30.9% and 15.8%, respectively, than the general US administration group (Group 1). ), Calcium and phosphorus concentrations were increased by about 57.1% and 27.1%, respectively, compared to the untreated group (first group). In addition, the group administered with oral administration of calcium fertilized rice and calcium preparation simultaneously (Group 4) showed synergistic effects by increasing calcium and phosphorus concentrations by about 59.3% and 30.6%, respectively, compared to the general US administration group (Group 1). 7). This reflects a reduced state of osteoporosis risk in the experimental group, it can be seen that due to the high calcium absorption of calcium fertilized rice and calcium preparations.

The preparation examples for the compositions of the present invention are illustrated below.

Preparation Example 1 Preparation of a Pharmaceutical Formulation

1. Preparation of powder

2 g shell ionized calcium

1 g lactose

The above components were mixed and packed in airtight bags to prepare powders.

2. Preparation of Tablets

Shell Calcium Ionization 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

Shell Calcium Ionization 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

4. Manufacture of rings

1 g of shell ionized calcium

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, it was prepared to be 4 g per ring in a conventional manner.

5. Manufacture of granules

Shell Calcium Ionized 150 mg

Soy extract 50 mg

Glucose 200 mg

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and dried at 60 ° C. to form granules, which were then filled into fabrics.

Preparation Example 2 Preparation of Food

Foods containing shell ionized calcium or shell ionized rice fertilized rice of the present invention were prepared as follows.

1. Preparation of flour food

0.5-5.0 parts by weight of shell-ionized calcium fertilized rice of the present invention was added to flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare foods for health promotion.

2. Preparation of soups and gravy

0.1-5.0 parts by weight of shell ionized calcium of the present invention was added to soups and broths to prepare meat products for health promotion, soups and noodles for noodles.

3. Preparation of Ground Beef

10 parts by weight of the shell ionized calcium of the present invention was added to the ground beef to prepare a ground beef for health promotion.

4. Manufacture of dairy products

5-10 parts by weight of shell ionized calcium fertilized rice of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

5. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then ground to a powder having a particle size of 60 mesh.

The shell-ionized calcium fertilized rice of the present invention was concentrated under reduced pressure in a vacuum concentrator, dried by spraying and drying with a hot air dryer, and ground to a particle size of 60 mesh by a grinder to obtain a dry powder.

The grains, seeds and shell-ionized calcium fertilized rice prepared above were prepared by combining the following ratios.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Shell ionized rice fertilized rice (3 parts by weight),

Ganoderma lucidum (0.5 parts by weight),

Foxglove (0.5 part by weight)

Preparation Example 3 Preparation of Beverage

1. Manufacture of health drinks

Instant sterilization by homogeneously mixing 5 g of shell ionized calcium of the present invention with subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%). After that, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare healthy drinks.

2. Preparation of Vegetable Juice

5 g of shell ionized calcium of the present invention was added to 1,000 ml of tomato or carrot juice to prepare a vegetable juice for health promotion.

3. Preparation of Fruit Juice

1 g of shell ionized calcium of the present invention was added to 1,000 ml of apple or grape juice to prepare a fruit juice for health promotion.

The calcium compound of the present invention can be usefully used for the development of drugs and health functional food for preventing and treating osteoporosis, and the rice prepared by fertilizing the calcium compound can also be usefully used for the development of health functional food for preventing and improving osteoporosis. have.

Claims (17)

Osteoporosis prevention and treatment composition containing calcium ion as an active ingredient. The composition for preventing and treating osteoporosis according to claim 1, wherein the calcium ionized is shell ionized calcium. The method of claim 1, wherein the calcium ionized 1) crushing the shell to a size of 1.5 to 10 cm; 2) after the crushed shell is loaded into the firing furnace, the temperature is gradually raised to 1200 ° C. to fire the shell; 3) The heat supply for heating of the kiln is cut off, and the cooled shell is gradually cooled while keeping the heated shell inside the kiln, until the temperature inside the kiln is 200 to 400 ° C, and then the door of the kiln is gradually opened to the kiln. What is produced by the manufacturing method comprising the step of cooling the internal temperature of the kiln to 100 to 150 ℃ while gradually entering the outside air, and then taking the shell out of the kiln and cooling in the atmosphere until the room temperature. Osteoporosis prevention and treatment composition characterized in. The composition for preventing and treating osteoporosis according to claim 1, wherein the calcium ion increases the concentration of calcium or osteocalcin in the blood. The method of claim 1, wherein the calcium ionized composition for preventing and treating osteoporosis, characterized in that to reduce the concentration of phosphorus or parathyroid hormone (PTH) in the blood. The method of claim 1, wherein the calcium ionized composition for preventing and treating osteoporosis, characterized in that to increase the concentration of calcium or phosphorus in the femur. A method of preventing or treating osteoporosis comprising administering to a subject a pharmaceutically effective amount of the composition of claim 1. Health functional food for osteoporosis prevention and improvement containing ionized calcium as an active ingredient. Rice having osteoporosis prevention and improvement effect prepared by fertilizing calcium ionized rice. 10. The rice having osteoporosis prevention and amelioration effect according to claim 9, wherein the calcium ionization is shell ionization. 10. The rice having osteoporosis prevention and improvement effect according to claim 9, wherein the concentration of calcium or osteocalcin in the blood is increased. 10. The rice having osteoporosis prevention and improvement effect according to claim 9, wherein the concentration of phosphorus or parathyroid hormone (PTH) in the blood is reduced. 10. The rice having osteoporosis prevention and improvement effect according to claim 9, wherein the concentration of calcium or phosphorus in the femur is increased. A method for preventing or improving osteoporosis, comprising orally administering to a subject a rice having an osteoporosis prevention and ameliorating effect prepared by fertilizing calcium ion with rice. A method of producing rice having an osteoporosis prevention and improvement effect, comprising the step of fertilizing calcium ion powder with fertilizer in rice. The method of claim 15, wherein the calcium ionized powder is administered by fertilizing with fertilizer three times from four weeks after rice seeding to just before the growth of ear, and three times from the time of harvesting of ear to harvesting. Eggplant rice manufacturing method. A health functional food for osteoporosis prevention and improvement containing rice having the effect of preventing and improving osteoporosis prepared by fertilizing calcium ion in rice.