CN104062503B - Data acquisition device for tissues in vitro impedance bioelectrical measurement - Google Patents
Data acquisition device for tissues in vitro impedance bioelectrical measurement Download PDFInfo
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Abstract
Data acquisition device for tissues in vitro impedance bioelectrical measurement, probe fixed plate including probe 7 and fixed probe 7, the rear end of every probe 7 is flexibly connected with the front end of a sliding bar B5, there are pressure sensor 6, the rear end of sliding bar B5 to be flexibly connected with probe fixed plate between sliding bar B5 and probe 7；The end face of the front end of every probe 7 is attachment face, has insulation surface layer close to the side of the front end of the probe 7 in attachment face.Eliminate the influence of pressure gap, conductive area difference to test result between different probe.
Description
Technical field
The present invention relates to a kind of adjustable test devices of probe pressure for tissues in vitro impedance bioelectrical measurement, relate to
And the bio-electrical impedance measuring field in biomedical engineering, it is mainly used for measuring the electrical impedance characteristics of in vitro biology.
Background technology
A basic physical parameter of the electrical impedance as biological tissue, obtains biophysicist and physiology for a long time
The extensive concern of scholar.The patch-clamp that largely uses in electrophysiology field at present, voltage clamp technology are based on cell membrane
Electrical impedance effect principle.Biological tissue is worth further investigation as a kind of dielectric, its electrical characteristics.The biophysics of early stage
Studies have shown that the difference for the resistance characteristic that biological tissue shows under DC state can be used for distinguishing different tissues；
And more it is worth noting that the electrical impedance of biological tissue shows prodigious variation with the difference of the frequency of applied electronic signal,
We term it impedance spectrums for this phenomenon.
In being measured to biological tissue's impedance spectrum, the method generally used has bipolar electrode method, four electrode method.Double electricity
Pole measuring technique is to introduce the alternating current of constant amplitude by a pair of electrodes to be tested biological tissue, then pass through same a pair of electrodes
The voltage detecting at its both ends is gone out.Since bipolar electrode is in use, the current density under electrode in tested tissue is higher than tested group
Knit the current density at other positions, i.e. current distribution is uneven is even, organizes various pieces just different to the contribution of impedance in this way, to
The impedance measured and practical impedance is set to have larger error.Meanwhile there is also contact resistances between electrode and biological tissue, and should
Resistance is also unstable, this will introduce measurement error again.In addition, electric current will also generate pole when flowing through electrode and biological tissue's electrolyte
Change phenomenon, polarisation error is than more serious in low frequency.Therefore, when accurately measuring biological tissue's impedance, bipolar electrode method is also few
See, is gradually replaced by four electrode method.
Typical four-probe measurement system includes two pairs of electrodes, a pair of electrodes（Galvanic electrode）By the alternation electricity of constant amplitude
Stream, which introduces, is tested biological tissue, another pair electrode（Voltage electrode）Between intervening two galvanic electrodes, the current potential for being tested position is detected
Difference.Since current electrode is detached with measuring electrode in four-probe measurement system, it is more equal that voltage electrode is in current density distribution
Even interlude, when using the operational amplifier of high input impedance, contact resistance between voltage electrode and tested tissue can be with
It ignores, while the polarization between electrode and biological tissue's electrolyte can also ignore.So four electrode method is relatively good
Overcome bipolar electrode method there are the problem of, so as to the biological impedance suitable for wider frequency rate.Currently, biological tissue
Impedance measurement is generally used four-probe measurement technology.
Due to the particularity of biological tissue, the complex impedance of in vitro tissue changes a lot as isolated time extends,
To a frequency sweep of in vitro tissue（Driving frequency is loaded into measured target successively by low frequency to high frequency or by high frequency to low frequency）It must
It must be completed within the time short enough.
Impedance spectroscopy measurement is carried out to tested biological tissue, the physical signs of tested tissue to be kept to be in plateau,
It cannot make the stained tested tissue such as secretion and blood, and cannot be rinsed with water, in order to avoid influence the accuracy of measurement data.
Chinese patent 201210425348.6 provides a kind of measuring box of biological in vitro tissue dielectric property, but when operation
Need to expend the time is sliced tested tissue, destroys the architectural characteristic of biological tissue, and need overlong time, and when measurement gives birth to
The complex impedance of object in vitro tissue has occurred and that variation, disturbs the accuracy of measurement result.
Chinese patent 200680019531.9 provides the probe for measuring human or animal body tissue impedance, but does not consider
It is not to give probe when measuring in complete flat state and various different tissues to slice mechanics of biological tissue surface
Different shrinkage degree when pressure, such as breast cancer tissue are that cancer cell constantly swallows periphery normal cell generation, organization center
Position consistency highest, inwardly collapses, and when histotomy does not accomplish substantially flat, causes the contact of electrode and tested biological tissue
It is bad or cannot completely attach to and keep measurement data inaccurate or measure unsuccessful；And electrode surface is to being tested the pressure of biological tissue too
Greatly, and retained secretion or blood from overflowing interference measurement in tissue can be caused.
In the case of no external pressure, the poor contact of electrode and tested biological tissue or it cannot completely attach to（It visits
The conducting surface of needle is often below attachment face）Keep measurement data inaccurate or measures unsuccessful；When giving external pressure, on the one hand,
After contacting compression with probe pressure deformation can occur for tested tissue, and the conductive capability of tested tissue can change because of pressure deformation,
Cause the precision of gathered data impacted, on the other hand, tested tissue is easy to contact with the survey face of probe after compression, leads to conduction
Face is more than attachment face, and conductive area is not known, the accuracy of interference measurement data yet.
Invention content
Each probe and tested biological tissue can be individually adjusted the purpose of the present invention is to provide a kind of on the contact surface
There is insulation surface layer in the side of pressure, the front end of probe, and the attachment face of each probe can be made to be attached at tested life with identical pressure
Object tissue surface makes reactive data of the tested biological tissue of extraction not by different probe and tested biological tissue surface pressure
Power variation, conducting surface change and impacted harvester.
It is fixed for the data acquisition device of tissues in vitro impedance bioelectrical measurement, including the probe of probe and fixed probe
Plate, the rear end of every probe are flexibly connected with the front end of a sliding bar B, there is pressure sensor between sliding bar B and probe, sliding
The rear end of lever B is flexibly connected with probe fixed plate, and the end face of the front end of every probe is attachment face, close to the probe in attachment face
The side of front end have insulation surface layer.
The above-mentioned data acquisition device for tissues in vitro impedance bioelectrical measurement, the rear end of the sliding bar B and spy
Carriage mounting hole in needle fixed plate is slidably matched, and has in probe fixed plate and pushes sliding bar B slidings and locking sliding bar B
Slide lock device.
The above-mentioned data acquisition device for tissues in vitro impedance bioelectrical measurement, the probe fixed plate are that lifting is flat
Platform has on hoistable platform sliding eye and locking device, the cooperation of sliding eye and sliding bar A to realize hoistable platform relative to sliding bar
The sliding of A realizes locking of the hoistable platform relative to sliding bar A by the cooperation of locking device and sliding bar A.
The above-mentioned data acquisition device for tissues in vitro impedance bioelectrical measurement, probe slip limiting plate are flat with lifting
Platform is fixedly connected, and has probe to slide limit hole in probe slip limiting plate, and probe is each passed through probe sliding limit hole, every spy
The probe sliding limit hole that needle is passed through with it is slidably matched.
The above-mentioned data acquisition device for tissues in vitro impedance bioelectrical measurement, one end of the sliding bar A are fixed
On pedestal.
Advantageous effect
The present invention forms four electrode probes by that can adjust as needed to the probe for being tested biological tissue's pressure, can be effective
The incomplete contact between of biological tissue surface generation or the measurement caused by poor contact for solving probe attachment face and out-of-flatness are lost
Lose the problem with measurement data inaccuracy；
Close to attachment face probe front end side insulation surface layer, when can make probe by identical pressure, not because
Cause conducting surface different with the difference of the density of the tested tissue of attached surfaces brought into contact, even if.The present invention realizes every spy
Head attachment face and tested tissue between pressure, conductive area it is consistent, to eliminate the pressure gap and conduction between different probe
Influence of the area discrepancy to test result improves measuring accuracy.
Because pressure sensor can provide pressure data in real time, the data can in data processing equipment in advance it is preset just
Regular data compares, to which the amendment of the data for acquisition provides reference.
" every probe of the present invention（7）Rear end and a sliding bar B（5）Front end flexible connection " refer to probe
（7）Rear end and a sliding bar B（5）Front end between connection do not influence the relative movement between the two, both in other words
Connection can make the two maintain connection relation and influence the two to the pressure sensor on pressure sensor mounting surface
It squeezes.
" probe of the present invention（7）Front end " one end where attachment face, the other end opposite with this one end is probe
（7）Rear end, " sliding bar B（5）Rear end " refer to that one end for being slidably matched with probe fixed plate, the other end is exactly to slide
Bar B（5）Front end, " close to attachment face probe（7）The side of front end have insulation surface layer " in insulation surface layer main work
With being to limit out every probe and the maximum conducting surface of tested tissue, so the insulation surface layer is close to attachment face.
" sliding bar B（5）Rear end be flexibly connected with probe fixed plate " refer to every sliding bar B（5）Rear end individually connect
Be connected to in probe fixed plate, but sliding bar B（5）Relative to probe fixed plate movement and probe can be driven again（7）It is flexible, mechanics
It is upper that there are many designs that this function can be achieved.
Five, attached drawing and attached drawing title
Attached drawing 1 is device stereoscopic schematic diagram
Attached drawing 2 is hoistable platform schematic diagram
Attached drawing 3 is probe schematic diagram
Attached drawing 4 is probe rod sectional view
1- pedestals, 2- sliding bars A, 3- hoistable platform, 4- slide lock devices, 5- sliding bars B, 6- pressure sensor,
7- probes, 8- locking devices, 9- sliding bar A sliding eyes, 10- probes slide limit hole, 11- carriage mounting holes, and 12- is visited
Shank, 13 insulation surface layers, 14- pressure sensor mounting surfaces.
Six, specific implementation mode
Embodiment 1：For the data acquisition device of tissues in vitro impedance bioelectrical measurement, including probe 7 and fixed probe 7
Probe fixed plate, the rear end of every probe 7 is flexibly connected with the front end of a sliding bar B5, between sliding bar B5 and probe 7
There are pressure sensor 6, the rear end of sliding bar B5 to be flexibly connected with probe fixed plate.
Device described in the present embodiment, the end face of the front end of every probe 7 be attachment face, close to the probe 7 in attachment face
There is insulation surface layer in the side of front end.
Probe, pressure sensor 6 are connected to the corresponding port of background process device with conducting wire.
When being tested using the present apparatus, tested biological tissue is placed under probe attachment face, tune meets every sliding bar B5, makes 4
Root probe 7 is consistent with the pressure of tested tissue, then startup power supply, receives data.
Embodiment 2：The present embodiment basic structure holds including placement tested as shown in Fig. 1 with embodiment 1, the present apparatus
The pedestal of biological tissue's vessel（1）, pedestal（1）It is upper that there is sliding bar 2, hoistable platform（3）Pass through sliding bar A sliding eyes thereon
（9）Along sliding bar（2）Required position is slided up and down, and passes through hoistable platform（3）On locking device（8）It is fixed.
Probe（7）Probe rod（12）Probe on hoistable platform slides limit hole（10）In, probe limit hole
It can make probe（7）It slides up and down without displacement, ensures probe（7）Between position it is constant to ensure the standard of test data
True property.
Pressure sensor（6）Be fixed on probe on one side（7）Pressure sensor mounting surface（14）On, another side and sliding
Bar B（5）It is fixedly connected, sliding bar B（5）By being mounted on hoistable platform（3）On slide lock device（4）It slides up and down, carries
Pressure force snesor（6）Data to obtain the pressure that tested biological tissue surface is given in probe test face.
By probe rod shown in the probe overall structure of the present apparatus such as attached drawing 3 and attached drawing 4（12）, insulation surface layer（13）Composition and
Pressure sensor mounting surface（14）Composition.
Four probes of the present apparatus（7）It is equidistantly in a row, it is mounted on hoistable platform（3）On with insulating materials make probe
Slide limit hole（10）In, probe tails are connected with conducting wire；The probe of both sides two（7）Constant amplitude is introduced as galvanic electrode
Alternating current is to tested biological tissue, intermediate two probes（7）The potential difference for being tested position is measured as voltage electrode.
The probe rod of the present apparatus（12）Bottom has insulation surface layer (13), can make every probe（7）With tested biological tissue
Conducting surface is consistent, and improves the accuracy of test data
Present apparatus hoistable platform（3）Sliding on sliding bar A (2) can be by optical axis and linear bearing realization, locking straight line
Bearing achieves that locking device（8）Function；Or can hoistable platform be driven by motor（3）Lifting and locking.
The probe of the present apparatus（7）Slide up and down and lock and can also be realized with above two mode, and can increase on optical axis
Add scale to read probe（7）Displacement distance, or directly obtained according to the rotational parameters of motor, to increase device to test data
Accuracy.
In above-described embodiment, the pedestal 1, for placing the vessel for holding tested biological tissue.
The sliding bar A（2）, for mobile lifting platform (3) to measurement position.
The hoistable platform (3), for fixing probe (7) and slide lock device (4).
The slide lock device (4) and sliding bar B（5）, required position is arrived for lifting fixed probe (7).
The pressure sensor (6), the pressure for testing each probe (7) to tested biological tissue.
The probe (7) is tested the signal of biological tissue for extracting input and extraction.
Testing Platform with slide-bar can avoid the human error as caused by measuring operator's hand hold transducer.
Claims (5)
1. for the data acquisition device of tissues in vitro impedance bioelectrical measurement, include the probe of probe (7) and fixed probe (7)
Fixed plate is characterized in that：The rear end of every probe (7) is flexibly connected with the front end of a sliding bar B (5), sliding bar B (5) with
There are pressure sensor (6), the rear end of sliding bar B (5) to be flexibly connected with probe fixed plate between probe (7)；Every probe (7)
The end face of front end is attachment face, has insulation surface layer (13) close to the side of the front end of the probe (7) in attachment face；The pressure sensing
Device (6) is used to test the pressure suffered by probe (7), it is ensured that the attachment face of every probe (7) is attached at tested with identical pressure
Biological tissue surface.
2. the data acquisition device according to claim 1 for tissues in vitro impedance bioelectrical measurement is characterized in that：It is sliding
Carriage mounting hole (11) on the rear end and probe fixed plate of lever B (5) is slidably matched, and has promotion to slide in probe fixed plate
Lever B (5) is slided and the slide lock device (4) of locking sliding bar B (5).
3. the data acquisition device according to claim 2 for tissues in vitro impedance bioelectrical measurement is characterized in that：Institute
It is hoistable platform (3) to state probe fixed plate, there is sliding eye (9) and locking device (8), sliding eye (9) and cunning on hoistable platform (3)
The cooperation of lever A (2) realizes hoistable platform (3) relative to the sliding of sliding bar A (2) or passes through locking device (8) and sliding bar A
(2) locking of the hoistable platform (3) relative to (2) sliding bar A is realized in cooperation.
4. the data acquisition device according to claim 3 for tissues in vitro impedance bioelectrical measurement is characterized in that：It visits
Needle slip limiting plate is fixedly connected with hoistable platform (3), has probe to slide limit hole (10), probe in probe slip limiting plate
(7) it is each passed through probe sliding limit hole (10), the probe sliding limit hole (10) that every probe (7) passes through with it, which slides, matches
It closes.
5. the data acquisition device according to claim 3 for tissues in vitro impedance bioelectrical measurement is characterized in that：It is sliding
One end of lever A (2) is fixed on pedestal (1).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310658841.7A CN104062503B (en) | 2013-12-09 | 2013-12-09 | Data acquisition device for tissues in vitro impedance bioelectrical measurement |
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| CN201310658841.7A CN104062503B (en) | 2013-12-09 | 2013-12-09 | Data acquisition device for tissues in vitro impedance bioelectrical measurement |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103654777B (en) * | 2013-12-09 | 2018-09-18 | 思澜科技（成都）有限公司 | Measure the device of organism electrical impedance |
| CN105606898A (en) * | 2016-03-21 | 2016-05-25 | 镇江博昊科技有限公司 | Graphite film four-probe resistance testing meter |
| US10761145B2 (en) | 2017-08-22 | 2020-09-01 | Wuhan China Star Optoelectronics Semiconductor Display Technology Co., Ltd. | Illuminating fixture and device |
| CN107578733A (en) * | 2017-08-22 | 2018-01-12 | 武汉华星光电半导体显示技术有限公司 | One kind lights tool and device |
| CN109030888A (en) * | 2018-07-18 | 2018-12-18 | 郑州云海信息技术有限公司 | A kind of probe load-bearing monitor method and pressure-sensitive probe |
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| CN1608586A (en) * | 2003-10-24 | 2005-04-27 | 欧姆龙健康医疗事业株式会社 | Impedance measuring apparatus and health management directing and suggesting apparatus |
| CN1739448A (en) * | 2005-08-04 | 2006-03-01 | 浙江大学 | Biological impedance, resistance or potential detecting electrode with dynamometer |
| CN201302593Y (en) * | 2008-11-25 | 2009-09-02 | 北京有色金属研究总院 | A fixing device for plane resistivity measurement of germanium crystal |
| CN102928670A (en) * | 2012-10-30 | 2013-02-13 | 中国人民解放军第四军医大学 | Dielectric property measuring box for cylindrical in vitro biological tissues |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP4092990B2 (en) * | 2002-09-06 | 2008-05-28 | 株式会社日立製作所 | Biological and chemical sample inspection equipment |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608586A (en) * | 2003-10-24 | 2005-04-27 | 欧姆龙健康医疗事业株式会社 | Impedance measuring apparatus and health management directing and suggesting apparatus |
| CN1739448A (en) * | 2005-08-04 | 2006-03-01 | 浙江大学 | Biological impedance, resistance or potential detecting electrode with dynamometer |
| CN201302593Y (en) * | 2008-11-25 | 2009-09-02 | 北京有色金属研究总院 | A fixing device for plane resistivity measurement of germanium crystal |
| CN102928670A (en) * | 2012-10-30 | 2013-02-13 | 中国人民解放军第四军医大学 | Dielectric property measuring box for cylindrical in vitro biological tissues |
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