CN103654777B - Measure the device of organism electrical impedance - Google Patents
Measure the device of organism electrical impedance Download PDFInfo
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- CN103654777B CN103654777B CN201310658789.5A CN201310658789A CN103654777B CN 103654777 B CN103654777 B CN 103654777B CN 201310658789 A CN201310658789 A CN 201310658789A CN 103654777 B CN103654777 B CN 103654777B
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Abstract
Measure the probe of organism electrical impedance, including probe rod 6, probe pilot sleeve 7, compression spring 8 and insulating layer 9；One end of probe rod 6 is attachment face 10, and probe rod 6 and 7 axial sliding fit of probe pilot sleeve, one end of compression spring 8 are withstood on 7 roof of probe pilot sleeve, and the other end of compression spring 8 withstands on the head of probe rod 6, and the side wall of probe rod 6 is insulating layer 9；4 above-mentioned probes are regularly arranged, and probe pilot sleeve 7 is fixed together to form probe 4；Probe pilot sleeve 7 in probe 4 is fixed on probe fixing device 3, and one end and the slide-bar 2 of probe fixing device 3 are slidably matched, one end of slide-bar 2 and being fixedly connected for pedestal 1, constitutes the device for measuring organism electrical impedance.Probe rod stretches because of the diastole of compression spring, it is ensured that the attachment face of 4 probe rods is all contacted with tested tissue surface, and insulating layer can limit the maximum conductive area that probe rod is contacted with tested tissue, to improve accuracy of data acquisition.
Description
Technical field
The present invention relates to the device for tissues in vitro impedance bioelectrical measurement, the bioelectricity that is related in biomedical engineering
Impedance measurement field.
Background technology
A basic physical parameter of the electrical impedance as biological tissue, obtains biophysicist and physiology for a long time
The extensive concern of scholar.The patch-clamp that largely uses in electrophysiology field at present, voltage clamp technology are based on cell membrane
Electrical impedance effect principle.Biological tissue is worth further investigation as a kind of dielectric, its electrical characteristics.The biophysics of early stage
Studies have shown that the difference for the resistance characteristic that biological tissue shows under DC state can be used for distinguishing different tissues；
And more it is worth noting that the electrical impedance of biological tissue shows prodigious variation with the difference of the frequency of applied electronic signal,
We term it impedance spectrums for this phenomenon.
In being measured to biological tissue's impedance spectrum, the method generally used has bipolar electrode method, four electrode method.Double electricity
Pole measuring technique is to introduce the alternating current of constant amplitude by a pair of electrodes to be tested biological tissue, then pass through same a pair of electrodes
The voltage detecting at its both ends is gone out.Since bipolar electrode is in use, the current density under electrode in tested tissue is higher than tested group
Knit the current density at other positions, i.e. current distribution is uneven is even, organizes various pieces just different to the contribution of impedance in this way, to
The impedance measured and practical impedance is set to have larger error.Meanwhile there is also contact resistances between electrode and biological tissue, and should
Resistance is also unstable, this will introduce measurement error again.In addition, electric current will also generate pole when flowing through electrode and biological tissue's electrolyte
Change phenomenon, polarisation error is than more serious in low frequency.Therefore, when accurately measuring biological tissue's impedance, bipolar electrode method is also few
See, is gradually replaced by four electrode method.
Typical four-probe measurement system includes two pairs of electrodes, and a pair of electrodes (galvanic electrode) is electric by the alternation of constant amplitude
Stream, which introduces, is tested biological tissue, between another pair electrode (voltage electrode) intervenes two galvanic electrodes, detects the current potential for being tested position
Difference.Since current electrode is detached with measuring electrode in four-probe measurement system, it is more equal that voltage electrode is in current density distribution
Even interlude, when using the operational amplifier of high input impedance, contact resistance between voltage electrode and tested tissue can be with
It ignores, while the polarization between electrode and biological tissue's electrolyte can also ignore.So four electrode method is relatively good
Customer service bipolar electrode method there are the problem of, so as to the biological impedance suitable for wider frequency rate.Currently, biological tissue
Impedance measurement is generally used four-probe measurement technology.
Due to the particularity of biological tissue, the complex impedance of in vitro tissue changes a lot as isolated time extends,
It must to a frequency sweep (driving frequency is loaded into measured target successively by low frequency to high frequency or by high frequency to low frequency) in vitro tissue
It must be completed within the time short enough.
Impedance spectroscopy measurement is carried out to tested biological tissue, the physical signs of tested tissue to be kept to be in plateau,
It cannot make the stained tested tissue such as secretion and blood, and cannot be rinsed with water, in order to avoid influence the accuracy of measurement data.
The time need to be expended to tested tissue when having the measuring box of biological in vitro tissue dielectric property in the prior art, but operating
It is sliced, destroys the architectural characteristic of biological tissue, and need overlong time, the complex impedance of biology in vitro tissue has been when measurement
Through changing, the accuracy of measurement result is disturbed.
It is useful for measuring the probe of human or animal body tissue impedance in the prior art, but does not consider slice biological tissue knot
Structure surface is not to be in contraction different when complete flat state and various different tissues give probe pressure when measuring
Degree, such as breast cancer tissue are that cancer cell constantly swallows periphery normal cell and generates, organization center's position consistency highest, to
It inside collapses, when histotomy does not accomplish substantially flat, causes the poor contact of electrode and tested biological tissue or cannot connect completely
Touching keeps measurement data inaccurate or measures unsuccessful；And electrode surface is increased to being tested the pressure of biological tissue, and tissue can be caused
Interior retained secretion or blood from overflowing interference measurement.
In addition, the conductive capability of probe is influenced toward contact by the conductive area size of probe and tested tissue contact surface,
Probe be pressurized after, tested tissue deforms upon, not only be attached face become conducting surface, close to attachment face probe side also because with quilt
It surveys tissue contact and becomes conducting surface, to keep the data of acquisition impacted by the variation of conducting surface.That is existing spy
After head is pressurized, the variation for the conductive area contacted with tested tissue of popping one's head in affects the acquisition precision of data.
Invention content
The probe provided by the invention for measuring organism electrical impedance, in use, probe rod can stretch, to realize probe
In the attachment face of probe rod can be contacted with tested tissue, while the conductive area popped one's head in is not increased by the increase of pressure.
Measure the probe of organism electrical impedance, including probe rod, probe pilot sleeve, compression spring and insulating layer；Probe
The end face of one end of bar is attachment face, probe rod and probe pilot sleeve axial sliding fit, and one end of compression spring withstands on spy
On needle pilot sleeve roof, the other end of compression spring withstands on the head of probe rod, and the side wall of probe rod is insulating layer.
The probe of above-mentioned measurement organism electrical impedance, has mutually in the side wall of probe pilot sleeve and the outer wall of probe rod
The position limiting structure of cooperation.
The probe of above-mentioned measurement organism electrical impedance, the probe include conducting wire, and conducting wire is oriented to probe rod or probe
Sleeve couples.
The probe of above-mentioned measurement organism electrical impedance, the insulating layer and attachment face flush.
The probe of organism electrical impedance is measured, the probe of 4 above-mentioned measurement organism electrical impedance, 4 probe rule are included
It then arranges, the probe pilot sleeve of 4 probes is fixed together.
The probe of above-mentioned measurement organism electrical impedance, regularly arranged 4 probes refer to that four probes equidistantly arrange
It is in a row.
Measure the device of organism electrical impedance, including pedestal, slide-bar, probe fixing device and above-mentioned probe；Slide-bar
One end is fixedly connected with pedestal；One end of probe fixing device is slidably matched with slide-bar, and the probe pilot sleeve in probe is solid
It is scheduled on probe fixing device.
The device of above-mentioned measurement organism electrical impedance, one end and the slide-bar of the probe fixing device are slidably matched, institute
State the locking structure that probe fixing device is equipped with locking slide-bar, the cunning of one end and slide-bar for probe locked fixing device
It is dynamic.
Advantageous effect：
The probe of organism electrical impedance is measured, in use, applying a power by probe pilot sleeve, this power passes through pressure
Power spring pushes the attachment face of probe rod to be contacted with tested tissue, behind the attachment face of probe rod and tested tissue contact, is tested
The reaction force that tissue is applied to probe rod by attachment face makes compression spring shrink, and probe rod is axially sliding with probe pilot sleeve
Dynamic cooperation, probe rod are upheld because the diastole of compression spring is with shrinking.
Side wall of the probe rod close to attachment face is insulating layer, which can limit probe rod and be contacted most with tested tissue
Big conductive area, to prevent probe rod from causing probe rod and tested tissue conductive contact surfaces because of the variation that it is under pressure
Variation, to improve the precision of data acquisition.
The probe of organism electrical impedance is measured, the probe pilot sleeve of 4 probes is fixed together, in use, 4 probes
Probe rod different contractions can occur, it can be achieved that 4 probes because of reaction force of its attachment face by tested tissue surface
The attachment face of bar is all contacted with tested tissue surface.
Measure the device of organism electrical impedance, pedestal 1, for placing the vessel for holding tested biological tissue；Slide-bar 2 is used
In lifting probe apparatus 4 to measurement position；Fixing device 3 is measuring position for fixing probe apparatus；Probe 4, for being attached
In measured tissues in vitro and provide pumping signal and extraction measurement data；Conducting wire 5, for connecting on probe apparatus 4
Four probes are to equipment.Testing Platform with slide-bar can avoid the human error as caused by measuring operator's hand hold transducer,
Caused by the incomplete contact between generated when the biological tissue surface that can effectively solve probe attachment and out-of-flatness or poor contact
Measure the problem of failure and measurement data inaccuracy.
Description of the drawings
Attached drawing 1 measures the device stereoscopic schematic diagram of organism electrical impedance
Attached drawing 2 is the probe stereoscopic schematic diagram for measuring organism electrical impedance
Attached drawing 3 is the probe pilot sleeve sectional view for measuring organism electrical impedance
Attached drawing 4 is the probe rod sectional view for measuring organism electrical impedance
Attached drawing 5 is the probe rod bottom enlarged drawing for measuring organism electrical impedance
Attached drawing 6 is the probe rod bottom, cross-sectional view for measuring organism electrical impedance
1- pedestals, 2- slide-bars, 3- fixing devices, 4- probes, 5- conducting wires, 6- probe rods, 7- probe pilot sleeves, 8- pressure
Spring, 9- insulating layers, 10- are attached face, 11- position limiting structures.
Specific implementation mode
Embodiment 1：Measure the probe of organism electrical impedance, including probe rod 6, probe pilot sleeve 7,8 and of compression spring
Insulating layer 9；One end of probe rod 6 is attachment face 10, probe rod 6 and 7 axial sliding fit of probe pilot sleeve, compression spring 8
One end withstand on 7 roof of probe pilot sleeve, the other end of compression spring 8 withstands on the head of probe rod 6, the side of probe rod 6
Wall is insulating layer 9.
Probe rod 6 shown in Fig. 2, Fig. 3 is inserted into one end that 7 axial sliding fit of probe pilot sleeve is probe rod 6
In probe pilot sleeve 7, and one end of probe pilot sleeve 7 be inserted into probe rod 6 in also can, for there is the skill of mechanical professional knowledge
For art personnel, there are many kinds of this axial sliding fits also.
The probe described in embodiment 1 for measuring organism electrical impedance, in side wall and the probe rod 6 of probe pilot sleeve 7
Outer wall has mutually matched position limiting structure.Position limiting structure shown in Fig. 2, Fig. 3 is the inner wall on the shell of probe pilot sleeve 7
On have an inside protrusion, the outer wall of probe rod 6 has one section of inside recessed limit, and protrusion can be in recessed limit along probe pilot sleeve 7
The right side wall (right side shown in figure one) of axial movement, recessed limit position can prevent probe rod 6 to be detached from probe pilot sleeve 7.
Restriction protrusion is on the inner wall of probe pilot sleeve 7 in above-mentioned position limiting structure, but restriction protrusion is in the outer of probe rod 6
On wall, the inner wall of probe pilot sleeve 7 is recessed outward, can also realize limit cooperation；Between probe rod 6 and probe pilot sleeve 7
Position limiting structure to further include other can not only prevent probe rod 6 to be detached from probe pilot sleeve 7, but also the end face of shank 6 can be made to be attached
It, can relative telescopic between probe rod 6 and probe pilot sleeve 7 when face 10 is pressurized：Such as：On the outer wall of probe pilot sleeve 7
The groove gaps of one or more strips is axially opened, being corresponded at groove gap on the outer wall of probe rod 6 has limit wing, which both can be
It is moved in groove gap, groove gap left end can block limit wing again prevents probe rod 6 to be detached from probe pilot sleeve 7.
Wing is limited in above-mentioned position limiting structure on the outer wall of probe rod 6, and is limited wing and be could be secured to probe pilot sleeve 7
Inner wall, as long as have on the outer wall of probe rod 6 cooperation limit wing movement groove body.
Certainly, in mechanics there are many position limiting structure, as long as can meet, make can between probe rod 6 and probe pilot sleeve 7
It is axially opposing flexible, and can prevent being slidably connected between probe rod 6 and probe pilot sleeve 7 from falling off just.
The probe includes conducting wire 5, and conducting wire 5 couples with probe rod 6 or probe pilot sleeve 7.Conducting wire either with probe
Pilot sleeve 7, or the connection with probe rod 6, if can beam conduction, not shadow to probe rod 6 and probe pilot sleeve 7 it
Between it is axially opposing it is flexible just.
The side wall of probe rod 6 described in embodiment 1 is insulating layer 9, refer to have outside the side wall close to 10 one end of attachment face one layer absolutely
Insulating layer 9, the conductive area that the presence of the insulating layer 9 can be such that probe rod 6 is contacted with tested tissue are limited.
The insulating layer (9) and attachment face (10) flush, the edge and the probe rod where attachment face 10 for being exactly insulating layer 9
6 end faces are consistent, and the presence of the insulating layer 9 can make the side wall of probe rod 6 not contact conduction with tested tissue, to make probe rod 6
And the conductive maximum area of tested tissue contact is exactly the area in the face that is attached 10.
Embodiment 2：Measure the probe of organism electrical impedance, including 4 described in embodiment 1 measure organism electrical impedance
Probe, 4 probes are regularly arranged, and the probe pilot sleeve (7) of 4 probes is fixed together.
4 probes described in the present embodiment are regularly arranged, refer to probe arrangement mode and data processing equipment in number
The arrangement mode arrangement determined according to the data collecting rule required by processing software.
The probe of measurement organism electrical impedance described in embodiment 2, described regularly arranged preferably four probes etc. of 4 probes
Spacing row are in a row.
Embodiment 3：Measure the device of organism electrical impedance, including pedestal 1, slide-bar 2, probe fixing device 3 and embodiment 2
The probe 4；One end of slide-bar 2 is fixedly connected with pedestal 1；One end of probe fixing device 3 is slidably matched with slide-bar 2,
Probe pilot sleeve 7 in probe 4 is fixed on probe fixing device 3.
The present apparatus includes placing the pedestal 1 for holding tested biological tissue's vessel as shown in Fig. 1, has slide-bar on pedestal 1
2, probe apparatus 4 is slided up and down along slide-bar 2, is fixed on required position by fixing device 3, four probes on probe apparatus 4
Attachment face 10 attach to tested biological tissue surface and measure.Four probes are in a row, and equidistant 1mm is highly flushed embedding
Enter in the mounting base made of insulating materials on probe apparatus 4, probe tails are connected with conducting wire；The probe of both sides two is as electric current
Electrode introduces the alternating current of constant amplitude to tested biological tissue, and intermediate two probes are measured as voltage electrode is tested position
Potential difference.
One end of the probe fixing device is slidably matched with slide-bar, and the probe fixing device is equipped with locking slide-bar
Locking structure, the sliding of one end and slide-bar for probe locked fixing device.
Probe structure is made of probe rod 6, probe pilot sleeve 7, compression spring 8, insulating layer 9 as shown in Fig. 2；Probe
Bar 6 can slide in the probe pilot sleeve 7 with screens slot 11, and the reset of probe rod 6 is controlled by compression spring 8.
The probe specific size of the present apparatus is as shown in Fig. 3.Probe rod 6 and probe pilot sleeve are made of silver alloy, visit
Shank 6 removes the insulating layer that attachment face outer surface is machined with 20um thickness along attachment face 10 to place outside probe pilot sleeve is exposed to, such as
Shown in attached drawing 4, when so that probe contact being tested biological tissue, remaining position is insulated with tested biological tissue in addition to being attached face 10,
The consistency for ensureing four probe contacts area, improves the accuracy of measurement.
The compression spring 8 of the present apparatus selects PHOSPHATE BRONZE WIRE to be made, and spring constant is that 0.01kgf/mm. passes through to spring constant
Selection, certain pressure applies probe rod 6 by compression spring 8 when measuring, makes attachment face 10 and tested biological tissue
It will not lead to remaining secretion or blood from overflowing in tested biological tissue when being effectively attached because of excessive pressure and interfere survey
Amount.
The size of present apparatus probe pilot sleeve 7 is as shown in Fig. 3, and probe pilot sleeve 7 is an outer diameter 1.12mm, internal diameter
The cylindrical drum of 0.82mm, away from the screens slot 11. for having an internal diameter at 8mm for 0.65mm that is open in the middle part of cylindrical drum
The size of the probe rod 6 of the present apparatus is as shown in Fig. 4, head diameter 1mm, mid diameter 0.5mm, and both ends are straight
Diameter is 0.76mm, since the internal diameter of the screens slot 11 on probe pilot sleeve 7 is less than both ends more than the mid diameter of probe rod 6
Diameter, so probe rod 6 can be slided in probe pilot sleeve 7 without the abjection out of pilot sleeve 7.
Attached drawing 6 is the enlarged cross-sectional view of 6 bottom of probe rod, and probe rod 6 is along attachment face 10 to being exposed to probe pilot sleeve
Outer place is machined with the insulating layer of 20um thickness except attachment face outer surface, as shown in Fig. 5, so that probe contact is tested biological tissue
When, remaining position is insulated with tested biological tissue in addition to being attached face 10, ensures the consistency of four probe contacts area, is improved and is surveyed
The accuracy of amount.
The compression spring 8 of the present apparatus selects PHOSPHATE BRONZE WIRE to be made, and spring constant is that 0.01kgf/mm. passes through to spring constant
Selection, certain pressure applies probe rod 6 by compression spring 8 when measuring, makes attachment face 10 and tested biological tissue
It will not lead to remaining secretion or blood from overflowing in tested biological tissue when being effectively attached because of excessive pressure and interfere survey
Probe rod 6 and 7 preferred silver alloy of probe pilot sleeve described in amount above-described embodiment are made.
The thickness of insulating layer described in above-described embodiment can change because of the difference of insulating materials, as long as isolation quilt can be played
Surveying tissue can with 6 side wall of probe rod contact conduction.
Claims (5)
1. measuring the device of organism electrical impedance, it is characterized in that：Including pedestal（1）, slide-bar（2）, probe fixing device（3）And spy
Head（4）；The probe includes the probe of 4 measurement organism electrical impedance, and the probe includes probe rod（6）, probe guide sleeve
Cylinder（7）, compression spring（8）And insulating layer（9）, the probe rod（6）One end end face be attachment face（10）, probe rod（6）
With probe pilot sleeve（7）Axial sliding fit, in probe pilot sleeve（7）Side wall and probe rod（6）Outer wall have mutually
The position limiting structure of cooperation, compression spring（8）One end withstand on probe pilot sleeve（7）On, compression spring（8）The other end withstand on
Probe rod（6）On, probe rod（6）Side wall be insulating layer（9）；4 probes are regularly arranged, the probe pilot sleeve of 4 probes
（7）It is fixed together, slide-bar（2）One end and pedestal（1）It is fixedly connected；Probe fixing device（3）One end and slide-bar（2）It is sliding
Dynamic cooperation, probe（4）In probe pilot sleeve（7）It is fixed on probe fixing device（3）On.
2. the device according to claim 1 for measuring organism electrical impedance, is characterized in that：4 probes are regularly arranged
It is in a row to refer to that four probes equidistantly arrange.
3. the device according to claim 1 for measuring organism electrical impedance, is characterized in that：The probe includes conducting wire（5）,
Conducting wire（5）With probe rod（6）Or probe pilot sleeve（7）Connection.
4. the device according to claim 1 for measuring organism electrical impedance, is characterized in that：The insulating layer（9）And attachment
Face（10）It flushes.
5. the device according to claim 1 for measuring organism electrical impedance, is characterized in that：The probe fixing device（3）
It is equipped with the structure of one end and slide-bar (2) sliding of probe locked fixing device (3).
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| CN201310658789.5A CN103654777B (en) | 2013-12-09 | 2013-12-09 | Measure the device of organism electrical impedance |
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| CN201310658789.5A CN103654777B (en) | 2013-12-09 | 2013-12-09 | Measure the device of organism electrical impedance |
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| CN103654777B true CN103654777B (en) | 2018-09-18 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104849558B (en) * | 2015-05-28 | 2018-01-16 | 东莞华贝电子科技有限公司 | Impedance measures component, impedance measuring equipment and impedance method for measurement |
| CN104873195A (en) * | 2015-06-19 | 2015-09-02 | 河南山之峰信息科技股份有限公司 | Human body resistance measuring device |
| CN105510650A (en) * | 2015-11-30 | 2016-04-20 | 广东长盈精密技术有限公司 | Probe and testing device comprising same |
| CN113782259A (en) * | 2021-08-23 | 2021-12-10 | 浙江柔灵科技有限公司 | Coating type conductive silica gel electrode antenna |
| CN117008070B (en) * | 2023-10-07 | 2023-12-19 | 成都世源频控技术股份有限公司 | Radar simulator with electric leakage detection function |
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| CN104062503A (en) * | 2013-12-09 | 2014-09-24 | 思澜科技（成都）有限公司 | Data acquisition device for measuring in vitro biological tissue electrical impedance |
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| FR2342502A1 (en) * | 1976-02-24 | 1977-09-23 | Sedat | Measurement of skin resistance - is performed by battery powered probe which compares resistance with potentiometer setting |
| GB2318642A (en) * | 1996-10-26 | 1998-04-29 | Stephen Magee | Self-adjustiing electrode for sensing galvanic skin resistance |
| RU2175850C2 (en) * | 1999-06-16 | 2001-11-20 | Саратовский научно-исследовательский институт сельской гигиены | Device for diagnosing allergic dermatoses |
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| CN201780334U (en) * | 2010-08-09 | 2011-03-30 | 深南电路有限公司 | Impedance test probe |
| TWI426275B (en) * | 2011-08-26 | 2014-02-11 | Pegatron Corp | Probe device |
| CN106706974B (en) * | 2016-12-23 | 2019-07-23 | 深圳市瑞能实业股份有限公司 | A kind of low resistance contacts conductive test electrode |
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| CN203609417U (en) * | 2013-12-09 | 2014-05-28 | 思澜科技（成都）有限公司 | Probe needle, probe and device for measuring electrical impedance of living organism |
| CN104062503A (en) * | 2013-12-09 | 2014-09-24 | 思澜科技（成都）有限公司 | Data acquisition device for measuring in vitro biological tissue electrical impedance |
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