CN103654777A - Probe, probe head and device for measuring impedance of living body - Google Patents
Probe, probe head and device for measuring impedance of living body Download PDFInfo
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- CN103654777A CN103654777A CN201310658789.5A CN201310658789A CN103654777A CN 103654777 A CN103654777 A CN 103654777A CN 201310658789 A CN201310658789 A CN 201310658789A CN 103654777 A CN103654777 A CN 103654777A
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Abstract
A probe for measuring the impedance of a living body comprises a probe rod 6, a probe guide sleeve 7, a pressure spring 8 and an insulating layer 9, wherein one end of the probe rod 6 is provided with an attaching face 10, the probe rod 6 is in slide fit with the probe guide sleeve 7 in the axial direction, one end of the pressure spring 8 abuts against the top wall of the probe guide sleeve 7, the other end of the pressure spring 8 abuts against the head portion of the probe rod 6, and the insulating layer 9 is arranged on the side wall of the probe rod 6. Four probes are regularly arranged, the probe guide sleeves 7 and the four probes are fixed together to form a probe head 4, the probe guide sleeves 7 in the probe head 4 are fixed to a probe head fixing device 3, one end of the probe head fixing device 3 is in slide fit with a slide shaft 2, one end of the slide shaft 2 is fixedly connected with a base 1, and therefore a device for measuring the impedance of the living body is formed. The probe rods are stretched out and drawn back due to relaxation of the pressure springs, and therefore the attaching faces of the four probe rods all make contact with the surface of tissue to be measured, the insulating layers can limit the largest contact electricity conduction area of the probe rods and the tissue to be measured, and data collection accuracy is improved.
Description
Technical field
The present invention relates to probe, probe and device for tissues in vitro impedance bioelectrical measurement, relate to the bio-electrical impedance measuring field in biomedical engineering.
Background technology
Electrical impedance, as one of biological tissue basic physical parameter, obtains the extensive concern of biophysicist and physiologist for a long time.The patch-clamp of using in a large number in electrophysiology field at present, voltage clamp technology be the electrical impedance effect principle based on cell membrane just.Biological tissue is as a kind of electrolyte, and its electrical characteristics are worth further investigation.Early stage biophysical studies shows, the difference of the resistance characteristic that biological tissue shows under DC state can be for distinguishing different tissues; And the electrical impedance that more it is worth noting biological tissue shows very large variation along with the difference of the frequency of the additional signal of telecommunication, we are referred to as impedance spectrum this phenomenon.
During Dui biological tissue impedance spectrum is measured, the general method adopting has bipolar electrode method, four electrode method.Bipolar electrode measuring technique is that the alternating current of constant amplitude is introduced to tested biological tissue by pair of electrodes, then by same pair of electrodes, the voltage detecting at its two ends is gone out.Due to bipolar electrode in use, electric current density under electrode in tested tissue is higher than the electric current density at other position of tested tissue, be that CURRENT DISTRIBUTION is inhomogeneous, organize like this various piece just different to the contribution of impedance, thereby make the impedance and the practical impedance that record have larger error.Meanwhile, also have contact resistance between electrode and biological tissue, and this resistance is also unstable, this will introduce measurement error again.In addition, when electric current flows through electrode and biological tissue's electrolyte, also will produce polarization phenomena, when low frequency, polarisation error is more serious.Therefore,, when accurately measuring biological tissue's impedance, bipolar electrode method is also rare, progressively by four electrode method, is replaced.
Typical four-probe measurement system comprises two pairs of electrodes, and pair of electrodes (galvanic electrode) is introduced tested biological tissue by the alternating current of constant amplitude, and another pair of electrodes (voltage electrode) gets involved between two galvanic electrodes, detects the potential difference at tested position.Because current electrode in four-probe measurement system is separated with measurement electrode, voltage electrode is in the more uniform interlude of electric current distribution, when adopting the operational amplifier of high input impedance, contact resistance between voltage electrode and tested tissue is negligible, and the polarization between electrode and biological tissue's electrolyte is also negligible simultaneously.So, the reasonable customer service of four electrode method the problem that exists of bipolar electrode method, thereby applicable to the biological impedance of wider frequency rate.At present, the impedance measurement of biological tissue generally all adopts four-probe measurement technology.
Particularity due to biological tissue, the complex impedance of in vitro tissue, along with isolated time extends and changes a lot, must complete in vitro tissue frequency sweep (driving frequency is loaded into measured target to high frequency or by high frequency to low frequency by low frequency successively) within the enough short time.
Tested biological tissue is carried out to impedance spectroscopy measurement, keep the physical signs of tested tissue in plateau, can not make secretions and blood etc. stain tested tissue, and can not rinse by water, in order to avoid affect the accuracy of measurement data.
The measuring box that has biological in vitro tissue dielectric property in prior art, but need expend time in tested tissue is cut into slices during operation, destroyed the architectural characteristic of biological tissue, and need overlong time, during measurement, the complex impedance of biological in vitro tissue changes, and has disturbed the accuracy of measurement result.
In prior art, be useful on the probe of measuring human or animal body tissue impedance, but reckoning without section mechanics of biological tissue surface is not in complete flat state, and various different tissues different shrinkage degree while giving probe pressure when measuring, for example breast cancer tissue is that cancerous cell is constantly engulfed the generation of periphery normal cell, organization center's position density is the highest, inwardly subside, during tissue slice, do not accomplish completely smoothly, cause the loose contact of electrode and tested biological tissue or can not contact completely to make measurement data inaccurate or measure unsuccessful; And strengthen the pressure of electrode surface to tested biological tissue, can cause again residual secretions or blood from overflowing interferometry in tissue.
In addition, the conductive capability of probe is subject to the impact of the conductive area size of probe and tested tissue contact surface toward contact, after probe pressurized, tested tissue generation deformation, not only attached face becomes conducting surface, near the probe side of attached also because of contacting and become conducting surface with tested tissue, thereby make the data of collection be subject to the variation of conducting surface and influenced.That is to say after existing probe pressurized, probe with the variable effect of the conductive area contacting with tested tissue the acquisition precision of data.
Summary of the invention
The probe of measurement organism electrical impedance provided by the invention, during use, probe rod can stretch, thus the attached face of realizing the probe rod in probe can contact with tested tissue, the increase that the conductive area of probe is not stressed simultaneously and increasing.
The probe of measuring organism electrical impedance, comprises probe rod, probe pilot sleeve, compression spring and insulating barrier; The end face of one end of probe rod is attached, probe rod and the axial sliding fit of probe pilot sleeve, and one end of compression spring withstands on probe pilot sleeve roof, and the other end of compression spring withstands on the head of probe rod, and the sidewall of probe rod is insulating barrier.
The probe of above-mentioned measurement organism electrical impedance, has at the sidewall of probe pilot sleeve and the outer wall of probe rod the position limiting structure cooperatively interacting.
The probe of above-mentioned measurement organism electrical impedance, described probe comprises wire, wire connects with probe rod or probe pilot sleeve.
The probe of above-mentioned measurement organism electrical impedance, described insulating barrier flushes with attached face.
Measure the probe of organism electrical impedance, comprise the probe of 4 above-mentioned measurement organism electrical impedance, 4 probes are regularly arranged, and the probe pilot sleeve of 4 probes is fixed together.
above-mentionedmeasure the probe of organism electrical impedance, described 4 probes are regularly arranged refers to that the equidistant row of four probes are in a row.
The device of measuring organism electrical impedance, comprises base, slide-bar, probe fixing device and above-mentioned probe; One end of slide-bar is fixedly connected with base; One end and the slide-bar of probe fixing device are slidably matched, and the probe pilot sleeve in probe is fixed on probe fixing device.
The device of above-mentioned measurement organism electrical impedance, one end of described probe fixing device and slide-bar are slidably matched with the structure of probe locked fixture and slide-bar.
Beneficial effect:
Measure the probe of organism electrical impedance, during use, by probe pilot sleeve, apply a power, this power promotes attached of probe rod by compression spring and contacts with tested tissue, after attached of probe rod contacts with tested tissue, the counteracting force that tested tissue imposes on probe rod by attached face shrinks compression spring, probe rod and the axial sliding fit of probe pilot sleeve, and probe rod stretches because of diastole and the contraction of compression spring.
Probe rod is insulating barrier near the sidewall of attached, this insulating barrier can limit the maximum conductive area that probe rod contacts with tested tissue, thereby prevent that probe rod from causing the variation of probe rod and tested tissue conductive contact surfaces because of its variation being under pressure, thereby improve the precision of data acquisition.
Measure the probe of organism electrical impedance, the probe pilot sleeve of 4 probes is fixed together, during use, can there is different contractions because of the counteracting force that its attached face is subject to tested tissue surface in the probe rod of 4 probes, the attached face that can realize 4 probe rods all with tested tissue Surface Contact; The existence of insulating barrier can make probe rod consistent with the conductive area of tested tissue contact.
The device of measuring organism electrical impedance, base 1, for placing the vessel that hold tested biological tissue; Slide-bar 2, arrives measuring position for lifting probe apparatus 4; Fixture 3, for static probe device in measuring position; Probe 4, for attaching to measured tissues in vitro and pumping signal being provided and extracting measurement data; Wire 5, for connecting four probes on probe apparatus 4 to equipment.Testing Platform with slide-bar, can avoid the personal error being caused by the measurement of operator's hand hold transducer, can effectively solve probe attached produce during with irregular biological tissue surface incomplete contact or measurement that loose contact causes unsuccessfully and the inaccurate problem of measurement data.
Accompanying drawing explanation
Accompanying drawing 2 is for measuring the probe schematic perspective view of organism electrical impedance
Accompanying drawing 3 is for measuring the probe pilot sleeve cutaway view of organism electrical impedance
Accompanying drawing 4 is for measuring the probe rod cutaway view of organism electrical impedance
Accompanying drawing 5 is for measuring the probe rod bottom enlarged drawing of organism electrical impedance
1-base, 2-slide-bar, 3-fixture, 4-probe, 5-wire, 6-probe rod, 7-probe pilot sleeve, 8-compression spring, 9-insulating barrier, attached of 10-, 11-position limiting structure.
The specific embodiment
embodiment 1:the probe of measuring organism electrical impedance, comprises probe rod 6, probe pilot sleeve 7, compression spring 8 and insulating barrier 9; One end of probe rod 6 is attached 10, probe rod 6 and 7 axial sliding fits of probe pilot sleeve, and one end of compression spring 8 withstands on probe pilot sleeve 7 roofs, and the other end of compression spring 8 withstands on the head of probe rod 6, and the sidewall of probe rod 6 is insulating barrier 9.
embodiment 1the probe of described measurement organism electrical impedance, has at the sidewall of probe pilot sleeve 7 and the outer wall of probe rod 6 position limiting structure cooperatively interacting.Position limiting structure shown in Fig. 2, Fig. 3 is to have an inside projection on the inwall on the shell of probe pilot sleeve 7, the outer wall of probe rod 6 has one section of inside recessed limit, projection can move axially along probe pilot sleeve 7 in recessed limit, and the right side wall at recessed limit position (right side shown in figure mono-) can prevent that probe rod 6 from departing from probe pilot sleeve 7.
In above-mentioned position limiting structure, restriction protrusion is on the inwall of probe pilot sleeve 7, but restriction protrusion is on the outer wall of probe rod 6, and the inwall of probe pilot sleeve 7 outwards caves in, and also can realize spacing cooperation;
In above-mentioned position limiting structure, spacing wing is on the outer wall of probe rod 6, and spacing wing also can be fixed on the inwall of probe pilot sleeve 7, as long as there is the cell body that coordinates spacing wing to move on the outer wall of probe rod 6.
Certainly, have many position limiting structures in mechanics, as long as can meet, make can be axially relative flexible between probe rod 6 and probe pilot sleeve 7, can prevent that again being slidably connected between probe rod 6 and probe pilot sleeve 7 from coming off just.
The probe of measurement organism electrical impedance according to claim 1, described probe comprises wire 5, wire 5 connects with probe rod 6 or probe pilot sleeve 7.Wire is no matter be and probe pilot sleeve 7, or with being connected of probe rod 6, as long as can normally conduct electricity, shadow is not to axially relative the stretching between probe rod 6 and probe pilot sleeve 7.
Described in embodiment 1, the sidewall of probe rod 6 is insulating barrier 9, and the sidewall of depending on nearly attached 10 one end has the exhausted insulating barrier 9 of one deck outward, and the conductive area that the existence of this insulating barrier 9 can make probe rod 6 contact with tested tissue is limited.
Described insulating barrier (9) flushes with attached (10), be exactly that the edge of insulating barrier 9 and probe rod 6 end faces at attached 10 places are consistent, the existence of this insulating barrier 9 can make the sidewall of probe rod 6 not contact conduction with tested tissue, thereby make conduction maximum area that probe rod 6 contacts with tested tissue, is exactly the area of attached 10.
Embodiment 2: measure the probe of organism electrical impedance, comprise the probe of 4 measurement organism electrical impedance described in embodiment 1,4 probes are regularly arranged, and the probe pilot sleeve (7) of 4 probes is fixed together.
4 probes described in the present embodiment are regularly arranged, refer to the arrangement mode of probe and the arrangement mode arrangement that the desired data collecting rule of the data processing software in data processing equipment determines.
The probe of the measurement organism electrical impedance described in embodiment 2, equidistantly row are in a row for regularly arranged preferably four probes of described 4 probes.
Embodiment 3: measure the device of organism electrical impedance, comprise the probe 4 described in base 1, slide-bar 2, probe fixing device 3 and embodiment 2; One end of slide-bar 2 is fixedly connected with base 1; One end of probe fixing device 3 and slide-bar 2 are slidably matched, and the probe pilot sleeve 7 in probe 4 is fixed on probe fixing device 3.
This device comprises as shown in Figure 1 places the base 1 that holds tested biological tissue vessel, on base 1, there is slide-bar 2, probe apparatus 4 slides up and down along slide-bar 2, by fixture 3, be fixed on desired location, attached 10 of four probes on probe apparatus 4 attaches to tested biological tissue surface and measures.Four probes are in a row, and equidistantly 1mm, highly flushes and embed on the mount pad of making of insulant on probe apparatus 4, and probe afterbody connects with wire; Two of both sides probe is introduced the alternating current of constant amplitude to tested biological tissue as galvanic electrode, middle two probes are measured the potential difference at tested position as voltage electrode.
One end of described probe fixing device and slide-bar are slidably matched with the structure of probe locked fixture and slide-bar.
Probe structure is comprised of probe rod 6, probe pilot sleeve 7, compression spring 8, insulating barrier 9 as shown in Figure 2; Probe rod 6 can have the interior slip of probe pilot sleeve 7 of screens groove 11, is controlled the reset of probe rod 6 by compression spring 8.
The concrete size of probe of this device as shown in Figure 3.Probe rod 6 and probe pilot sleeve are made by silver alloy, probe rod 6 is located outside being exposed to probe pilot sleeve along attached 10 to be processed with except attached outer surface the insulating barrier that 20um is thick, as shown in Figure 4, so that probe is while contacting tested biological tissue, all the other positions and the insulation of tested biological tissue except attached 10, guarantee the concordance of four probe contacts area, improve the accuracy of measuring.
The compression spring 8 of this device selects PHOSPHATE BRONZE WIRE to make, spring constant is that 0.01kgf/mm. passes through the selection to spring constant, when measuring, by 8 pairs of probe rods 6 of compression spring, apply certain pressure, make attached 10 can not cause because of excessive pressure secretions residual in tested biological tissue or blood from overflowing and interferometry with tested biological tissue is effective when attached.
As shown in Figure 3, probe pilot sleeve 7 is an external diameter 1.12mm to the size of this device probe pilot sleeve 7, the cylindrical drum of internal diameter 0.82mm, and at cylindrical drum middle part, having an internal diameter apart from opening 8mm place is the screens groove 11. of 0.65mm
The size of the probe rod 6 of this device as shown in Figure 4, head diameter is 1mm, mid diameter is 0.5mm, two ends diameter is 0.76mm, the middle part diameter that is greater than probe rod 6 due to the internal diameter of the screens groove 11 on probe pilot sleeve 7 is less than two ends diameter, so probe rod 6 can not deviate from the interior slip of probe pilot sleeve 7 in pilot sleeve 7.
The compression spring 8 of this device selects PHOSPHATE BRONZE WIRE to make, spring constant is that 0.01kgf/mm. passes through the selection to spring constant, when measuring, by 8 pairs of probe rods 6 of compression spring, apply certain pressure, make attached 10 can not cause because of excessive pressure secretions residual in tested biological tissue or blood from overflowing and interferometry with tested biological tissue is effective when attached
The thickness of the insulating barrier described in above-described embodiment can change because of the difference of insulant, as long as can play isolated tested tissue, conducts electricity and just can with probe rod 6 sidewall contacts.
Claims (8)
1. measure the probe of organism electrical impedance, be characterised in that: described probe comprises probe rod (6), probe pilot sleeve (7), compression spring (8) and insulating barrier (9); The end face of one end of probe rod (6) is attached (10), probe rod (6) and probe pilot sleeve (7) axial sliding fit, one end of compression spring (8) withstands on probe pilot sleeve (7), it is upper that the other end of compression spring (8) withstands on probe rod (6), and the sidewall of probe rod (6) is insulating barrier (9).
2. the probe of measurement organism electrical impedance according to claim 1, is characterised in that: at the sidewall of probe pilot sleeve (7) and the outer wall of probe rod (6), have the position limiting structure cooperatively interacting.
3. the probe of measurement organism electrical impedance according to claim 1, is characterised in that: described probe comprises wire (5), and wire (5) connects with probe rod (6) or probe pilot sleeve (7).
4. the probe of measurement organism electrical impedance according to claim 1, is characterised in that: described insulating barrier (9) flushes with attached (10).
5. measure the probe of organism electrical impedance, be characterised in that: comprise the probe of 4 measurement organism electrical impedance claimed in claim 1,4 probes are regularly arranged, the probe pilot sleeve (7) of 4 probes is fixed together.
6. measure the probe of organism electrical impedance, be characterised in that: described 4 probes are regularly arranged refers to that the equidistant row of four probes are in a row.
7. measure the device of organism electrical impedance, be characterised in that: comprise base (1), slide-bar (2), probe fixing device (3) and probe claimed in claim 5 (4); One end of slide-bar (2) is fixedly connected with base (1); One end of probe fixing device (3) and slide-bar (2) are slidably matched, and the probe pilot sleeve (7) in probe (4) is fixed on probe fixing device (3).
8. measure the device of organism electrical impedance, be characterised in that: one end of described probe fixing device (3) and slide-bar (2) are slidably matched with the structure of probe locked fixture (3) and slide-bar (2).
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104849558A (en) * | 2015-05-28 | 2015-08-19 | 东莞华贝电子科技有限公司 | Impedance measuring component, impedance measuring device and impedance measuring method |
CN104873195A (en) * | 2015-06-19 | 2015-09-02 | 河南山之峰信息科技股份有限公司 | Human body resistance measuring device |
CN105510650A (en) * | 2015-11-30 | 2016-04-20 | 广东长盈精密技术有限公司 | Probe and testing device comprising same |
CN113782259A (en) * | 2021-08-23 | 2021-12-10 | 浙江柔灵科技有限公司 | Coating type conductive silica gel electrode antenna |
CN117008070A (en) * | 2023-10-07 | 2023-11-07 | 成都世源频控技术股份有限公司 | Radar simulator with electric leakage detection function |
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CN106706974A (en) * | 2016-12-23 | 2017-05-24 | 深圳市瑞能实业股份有限公司 | Low-impedance contact conductive test electrode |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104849558A (en) * | 2015-05-28 | 2015-08-19 | 东莞华贝电子科技有限公司 | Impedance measuring component, impedance measuring device and impedance measuring method |
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CN105510650A (en) * | 2015-11-30 | 2016-04-20 | 广东长盈精密技术有限公司 | Probe and testing device comprising same |
CN113782259A (en) * | 2021-08-23 | 2021-12-10 | 浙江柔灵科技有限公司 | Coating type conductive silica gel electrode antenna |
CN117008070A (en) * | 2023-10-07 | 2023-11-07 | 成都世源频控技术股份有限公司 | Radar simulator with electric leakage detection function |
CN117008070B (en) * | 2023-10-07 | 2023-12-19 | 成都世源频控技术股份有限公司 | Radar simulator with electric leakage detection function |
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