CN104062503A - Data acquisition device for measuring in vitro biological tissue electrical impedance - Google Patents
Data acquisition device for measuring in vitro biological tissue electrical impedance Download PDFInfo
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- CN104062503A CN104062503A CN201310658841.7A CN201310658841A CN104062503A CN 104062503 A CN104062503 A CN 104062503A CN 201310658841 A CN201310658841 A CN 201310658841A CN 104062503 A CN104062503 A CN 104062503A
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Abstract
A data acquisition device for measuring in vitro biological tissue electrical impedance comprises probes 7 and a probe fixing plate for fixing the probes 7; the rear end of every probe 7 is movably connected with the front end of a sliding bar B5, a pressure sensor 6 is arranged between the sliding bar B5 and the probe 7, and the rear end of the sliding bar B5 is movably connected with the probe fixing plate; the end surface of the front end of every probe 7 is an attached surface, and an insulation surface is arranged close to the attached surface on the side of the front end of the probe 7. The data acquisition device for measuring in vitro biological tissue electrical impedance eliminates the effects from the pressure difference and the conductive area difference between different probes on measurement results.
Description
Technical field
The present invention relates to a kind of adjustable proving installation of probe pressure for tissues in vitro impedance bioelectrical measurement, relate to the bio-electrical impedance measuring field in biomedical engineering, be mainly used in the electrical impedance characteristics of in vitro biology to measure.
background technology
Electrical impedance, as one of biological tissue basic physical parameter, obtains biophysicist and physiologist's extensive concern for a long time.The patch-clamp of using in a large number in electrophysiology field at present, voltage clamp technology be the electrical impedance effect principle based on cell membrane just.Biological tissue is as a kind of dielectric, and its electrical characteristics are worth further investigation.Early stage biophysical studies shows, the difference of the resistance characteristic that biological tissue shows under DC state can be for distinguishing different tissues; And the electrical impedance that more it is worth noting biological tissue shows very large variation along with the difference of the frequency of additional electric signal, we are referred to as impedance spectrum this phenomenon.
During Dui biological tissue impedance spectrum is measured, the general method adopting has bipolar electrode method, four electrode method.Bipolar electrode measuring technique is that the exchange current of constant amplitude is introduced to tested biological tissue by pair of electrodes, then by same pair of electrodes, the voltage detecting at its two ends is gone out.Due to bipolar electrode in use, current density under electrode in tested tissue is higher than the current density at other position of tested tissue, be that distribution of current is inhomogeneous, organize like this various piece just different to the contribution of impedance, thereby make the impedance and the practical impedance that record have larger error.Meanwhile, also have contact resistance between electrode and biological tissue, and this resistance is also unstable, this will introduce measuring error again.In addition, when electric current flows through electrode and biological tissue's electrolytic solution, also will produce polarization phenomena, when low frequency, polarisation error is more serious.Therefore,, when accurately measuring biological tissue's impedance, bipolar electrode method is also rare, progressively by four electrode method, is replaced.
Typical four-probe measurement system comprises two pairs of electrodes, and pair of electrodes (galvanic electrode) is introduced tested biological tissue by the exchange current of constant amplitude, and another pair of electrodes (voltage electrode) gets involved between two galvanic electrodes, detects the potential difference (PD) at tested position.Because transmitting electrode in four-probe measurement system is separated with potential electrode, voltage electrode is in the more uniform interlude of electric current distribution, when adopting the operational amplifier of high input impedance, contact resistance between voltage electrode and tested tissue is negligible, and the polarization between electrode and biological tissue's electrolytic solution is also negligible simultaneously.So four electrode method is reasonable has overcome the problem that bipolar electrode method exists, thereby applicable to the biological impedance of wider frequency rate.At present, the impedance measurement of biological tissue generally all adopts four-probe measurement technology.
Singularity due to biological tissue, the complex impedance of in vitro tissue, along with isolated time extends and changes a lot, must complete in vitro tissue frequency sweep (driving frequency is loaded into measured target to high frequency or by high frequency to low frequency by low frequency successively) within the enough short time.
Tested biological tissue is carried out to impedance spectroscopy measurement, keep the physical signs of tested tissue in plateau, can not make secretion and blood etc. stain tested tissue, and can not rinse by water, in order to avoid affect the accuracy of measurement data.
Chinese patent 201210425348.6 provides a kind of measuring box of biological in vitro tissue dielectric property, but need expend time in tested tissue is cut into slices during operation, destroyed the architectural characteristic of biological tissue, and need overlong time, during measurement, the complex impedance of biological in vitro tissue changes, and has disturbed the accuracy of measurement result.
Chinese patent 200680019531.9 provides for measuring the probe of human or animal body tissue impedance, but reckoning without section mechanics of biological tissue surface is not in complete flat state, and various different tissues different shrinkage degree while giving probe pressure when measuring, for example breast cancer tissue is that cancer cell is constantly engulfed the generation of periphery normal cell, organization center's position density is the highest, inwardly subside, during histotomy, do not accomplish completely smooth, causing the loose contact of electrode and tested biological tissue or can not contacting completely makes measurement data inaccurate or measure unsuccessful, and electrode surface is too large to the pressure of tested biological tissue, can cause again residual secretion or blood from overflowing interferometry in tissue.
In the situation that there is no external pressure, the loose contact of electrode and tested biological tissue or can not contact (conducting surface of probe is often less than attached face) completely and make measurement data inaccurate or measure unsuccessful; While giving external pressure, on the one hand, after contacting pressurized with probe, tested tissue can there is pressure deformation, the conductive capability of tested tissue can change because of pressure deformation, causes the precision of image data influenced, on the other hand, after pressurized, tested tissue easily contacts with the survey face of probe, cause conducting surface to be greater than attached, conductive area uncertain, the also accuracy of interferometry data.
summary of the invention
The object of the present invention is to provide a kind of can
regulate separately each probe and quiltsurvey the pressure of biological tissue on surface of contact, there is insulation top layer the side of the front end of probe, can make attached of each probe with identical pressure plaster, to invest tested biological tissue surface, make reactive data of the tested biological tissue of extraction not changed and affected harvester by different probe and the surface pressing variation of tested biological tissue, conducting surface.
Data collector for tissues in vitro impedance bioelectrical measurement, the probe fixed head that comprises probe and stationary probe, the rear end of every probe is flexibly connected with the front end of a sliding bar B, between sliding bar B and probe, there is pressure transducer, the rear end of sliding bar B is flexibly connected with probe fixed head, the end face of the front end of every probe is attached, and there is insulation top layer the side of front end that is close to the probe of attached.
onthe data collector for tissues in vitro impedance bioelectrical measurement of stating, the carriage mounting hole on the rear end of described sliding bar B and probe fixed head is slidably matched, and has and promote the slide lock device that sliding bar B slides and locks sliding bar B on probe fixed head.
onthe data collector for tissues in vitro impedance bioelectrical measurement of stating, described probe fixed head is hoistable platform, on hoistable platform, have sliding eye and locking device, sliding eye with realize hoistable platform coordinating of sliding bar A with respect to the slip of sliding bar A or by locking device and coordinating of sliding bar A realizing hoistable platform with respect to the locking of sliding bar A.
onthe data collector for tissues in vitro impedance bioelectrical measurement of stating, probe slip limiting plate is fixedly connected with hoistable platform, on probe slip limiting plate, there is probe slip spacing hole, probe is respectively through probe slip spacing hole, and the probe slip spacing hole that every probe passes with it is slidably matched.
onthe data collector for tissues in vitro impedance bioelectrical measurement of stating, one end of described sliding bar A is fixed on base.
beneficial effect
The present invention forms four electrode probes by regulating as required to the probe of tested biological tissue pressure, can effectively solve that attached of probe produces with irregular biological tissue surface incomplete contact or measurement that loose contact causes unsuccessfully and the inaccurate problem of measurement data;
Be close to the insulation top layer of side of front end of the probe of attached, in the time of can making probe be subject to identical pressure, not because the different conducting surfaces that cause of the density of the tested tissue from attached contact are different, even.The present invention has realized the consistent of pressure, conductive area between attached of every probe and tested tissue, thereby has got rid of pressure gap and the impact of conductive area difference on test result between different probe, raising measuring accuracy.
Because pressure transducer can provide pressure data in real time, these data can be in data processing equipment and in advance preset normal data comparison, thereby provides reference for the correction of the data that gather.
" rear end of every probe (7) and a sliding bar B(5) front end be flexibly connected " of the present invention refers to the rear end and a sliding bar B(5 of probe (7)) front end between be connected and do not affect relatively moving of the two, the connection of the two in other words can make the two maintain annexation, does not affect again the two to being positioned at the extruding of the pressure transducer on pressure transducer installed surface.
The one end at " front end of probe (7) " of the present invention attached place, the other end relative with this one end is the rear end of probe (7), " sliding bar B(5) rear end " refers to and that one end being slidably matched with probe fixed head, the other end is exactly sliding bar B(5) front end, the Main Function on the insulation top layer in " there is insulation top layer the side of front end that is close to the probe (7) of attached " is the maximum conducting surface that limits out every probe and tested tissue, so this insulation top layer is near attached.
" sliding bar B(5) rear end be flexibly connected with probe fixed head " refers to every sliding bar B(5) rear end be connected to separately with probe fixed head on, but sliding bar B(5) can move and drive probe (7) flexible relative to probe fixed head again, in mechanics, have the multiple design that realizes this function.
five, accompanying drawing and accompanying drawing title
Accompanying drawing 1 is device schematic perspective view
Accompanying drawing 2 is hoistable platform schematic diagram
Accompanying drawing 3 is probe schematic diagram
Accompanying drawing 4 is probe rod cut-open view
1-base, 2-sliding bar A, 3-hoistable platform, 4-slide lock device, 5-sliding bar B, 6-pressure transducer, 7-probe, 8-locking device, 9-sliding bar A sliding eye, 10-probe slip spacing hole, 11-carriage mounting hole, 12-probe rod, 13 insulation top layers, 14-pressure transducer installed surface.
six, embodiment
embodiment 1:data collector for tissues in vitro impedance bioelectrical measurement, the probe fixed head that comprises probe 7 and stationary probe 7, the rear end of every probe 7 is flexibly connected with the front end of a sliding bar B5, between sliding bar B5 and probe 7, have pressure transducer 6, the rear end of sliding bar B5 is flexibly connected with probe fixed head.
Device described in the present embodiment, the end face of the front end of every probe 7 is attached, there is insulation top layer the side of front end that is close to the probe 7 of attached.
With wire, probe, pressure transducer 6 are connected to the corresponding port of background process device.
While using this device to test, tested biological tissue is placed under attached of probe, every sliding bar B5 of transfer, makes 4 probes 7 consistent with the pressure of tested tissue, then starts power supply, receives data.
embodiment 2:this
embodiment basic structure is with embodiment 1,this device comprises as shown in Figure 1 places the base (1) that holds tested biological tissue vessel, base has sliding bar 2 on (1), hoistable platform (3) slides up and down desired location by the sliding bar A sliding eye (9) on it along sliding bar (2), and fixing by the locking device (8) on hoistable platform (3).
The probe rod (12) of probe (7) is arranged in the probe slip spacing hole (10) on hoistable platform, probe spacing hole can make probe (7) slide up and down and not be subjected to displacement, guarantee probe (7) thus between invariant position guarantee the accuracy of test data.
The one side of pressure transducer (6) is fixed on the pressure transducer installed surface (14) of probe (7), another side and sliding bar B(5) be fixedly connected with, sliding bar B(5) by the slide lock device (4) being arranged on hoistable platform (3), slide up and down, extract pressure transducer (6) thus data obtain the pressure that probe test face gives tested biological tissue surface.
The probe one-piece construction of this device is comprised of and pressure transducer installed surface (14) forms probe rod (12), insulation top layer (13) as shown in accompanying drawing 3 and accompanying drawing 4.
Four probes of this device (7) are equidistantly in a row, are arranged in the upper probe slip spacing hole (10) of making of insulating material of hoistable platform (3), and probe afterbody connects with wire; Two probes in both sides (7) are introduced the exchange current of constant amplitude to tested biological tissue as galvanic electrode, middle two probes (7) are measured the potential difference (PD) at tested position as voltage electrode.
The probe rod of this device (12) bottom has insulation top layer (13), can make every probe (7) and the conducting surface of tested biological tissue be consistent, and improves the accuracy of test data
The slip of this device hoistable platform (3) on sliding bar A (2) can be realized by optical axis and linear bearing, and locking linear bearing just can be realized the function of locking device (8); Or can be by lifting and the locking of driven by motor hoistable platform (3).
Sliding up and down and lock also of the probe of this device (7) can realize by above-mentioned two kinds of modes, and on optical axis, can increase scale and read probe (7) displacement, or directly according to the rotational parameters of motor, draws, thereby increases the accuracy of device to test data.
in above-described embodiment,described base 1, for placing the vessel that hold tested biological tissue.
Described sliding bar A(2), for mobile lifting platform (3), arrive measuring position.
Described hoistable platform (3), for stationary probe (7) and slide lock device (4).
Described slide lock device (4) and sliding bar B(5), for lifting stationary probe (7), arrive desired location.
Described pressure transducer (6), for testing the pressure of each probe (7) to tested biological tissue.
Described probe (7), for extracting input and extracting the signal of tested biological tissue.
With the Testing Platform of slide-bar, can avoid the personal error being caused by the measurement of operator's hand hold transducer.
Claims (5)
1. for the data collector of tissues in vitro impedance bioelectrical measurement, the probe fixed head that comprises probe (7) and stationary probe (7), be characterised in that: the rear end of every probe (7) and a sliding bar B(5) front end be flexibly connected, sliding bar B(5) and between probe (7), have pressure transducer (6), a sliding bar B(5) rear end be flexibly connected with probe fixed head; The end face of the front end of every probe (7) is attached, and there is insulation top layer (13) side of front end that is close to the probe (7) of attached.
2. the data collector for tissues in vitro impedance bioelectrical measurement according to claim 1, be characterised in that: the carriage mounting hole (11) on rear end sliding bar B(5) and probe fixed head and probe fixed head are slidably matched on probe fixed head, have and promote sliding bar B(5) slide and locking sliding bar B(5) slide lock device (4).
3. the data collector for tissues in vitro impedance bioelectrical measurement according to claim 2, be characterised in that: described probe fixed head is hoistable platform (3), on hoistable platform (3), have sliding eye (9) and locking device (8), sliding eye (9) and sliding bar A(2) coordinate realize hoistable platform (3) with respect to sliding bar A(2) slip or by locking device (8) and sliding bar A(2) coordinate realize hoistable platform (3) with respect to sliding bar A(2) locking.
4. the data collector for tissues in vitro impedance bioelectrical measurement according to claim 3, be characterised in that: probe slip limiting plate is fixedly connected with hoistable platform (3), on probe slip limiting plate, there is probe slip spacing hole (10), probe (7) is respectively through probe slip spacing hole (10), and the probe slip spacing hole (10) that every probe (7) passes with it is slidably matched.
5. the data collector for tissues in vitro impedance bioelectrical measurement according to claim 3, is characterised in that: one end sliding bar A(2) is fixed on base (1).
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| CN201310658841.7A CN104062503B (en) | 2013-12-09 | 2013-12-09 | Data acquisition device for tissues in vitro impedance bioelectrical measurement |
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| CN201310658841.7A CN104062503B (en) | 2013-12-09 | 2013-12-09 | Data acquisition device for tissues in vitro impedance bioelectrical measurement |
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| CN104062503B CN104062503B (en) | 2018-09-18 |
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Cited By (5)
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| CN103654777A (en) * | 2013-12-09 | 2014-03-26 | 思澜科技（成都）有限公司 | Probe, probe head and device for measuring impedance of living body |
| CN105606898A (en) * | 2016-03-21 | 2016-05-25 | 镇江博昊科技有限公司 | Graphite film four-probe resistance testing meter |
| CN107578733A (en) * | 2017-08-22 | 2018-01-12 | 武汉华星光电半导体显示技术有限公司 | One kind lights tool and device |
| CN109030888A (en) * | 2018-07-18 | 2018-12-18 | 郑州云海信息技术有限公司 | A kind of probe load-bearing monitor method and pressure-sensitive probe |
| US10761145B2 (en) | 2017-08-22 | 2020-09-01 | Wuhan China Star Optoelectronics Semiconductor Display Technology Co., Ltd. | Illuminating fixture and device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109030888A (en) * | 2018-07-18 | 2018-12-18 | 郑州云海信息技术有限公司 | A kind of probe load-bearing monitor method and pressure-sensitive probe |
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