CN104054828B - Infant formula of a kind of anti-albumen allergy and preparation method thereof - Google Patents
Infant formula of a kind of anti-albumen allergy and preparation method thereof Download PDFInfo
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Abstract
The invention provides infant formula of a kind of anti-albumen allergy and preparation method thereof, described formula powder contains lactalbumin hydrolysate, described lactalbumin hydrolysate is prepared by following method: every 5.0g concentrated whey protein powder is dissolved in 95ml distilled water, in 85 DEG C of water-baths, keep after 20min, regulating PH is 7.0, add trypsase (vigor 25000U/g), keep 40 DEG C of hydrolysis temperatures, after hydrolysis 4h, 85 DEG C of heating water bath 10min of hydrolyzate enzyme that goes out, the centrifugal precipitation of going, regulate PH8.5, add aminopeptidase (vigor 10000U/g), 50 DEG C of hydrolysis temperatures, continue hydrolysis 6h, this formula powder lactalbumin hydrolysate used is lactalbumin depth hydrolysis thing, degree of hydrolysis reaches 48-55%, the absorption easy to digest of hydrolysis gained whey protein peptide, without sensitizing property, has been eliminated the bitter taste after lactalbumin depth hydrolysis simultaneously, and taste is easy to infant and accepts.
Description
Technical field
The present invention relates to a kind of infant formula, especially a kind of infant formula of anti-albumen allergy.
Background technology
Milk protein is first part of food proteins of formula milk feeding infant picked-up normally, is also the most often by reallyFixed food allergen. The baby of milk protein allergy (cmpa) conventionally there will be vomiting, chronic diarrhea,The symptom such as malabsorption and growth retardation. Milk protein allergy refers to a kind of IgE or non-IgE mediationThe bad reaction to milk protein, within 2 years old, following baby's incidence is about 2%. If cmpa infant can not getCorrect diagnosis and treatment, the cmpa infant month more than 1/2 will develop into many food hypersenstivities, have a strong impact on infant andThe quality of life of its family. Up to now, the absorption of strictly avoiding milk protein is that cmpa is unique has in treatmentThe method of effect. Therefore substitute original formulation for infant and the early stage selecting properly muting sensitive of children formula, improveAnd promote the nutrition of cmpa infant and grow to seem particularly important.
Present stage, the treatment of milk protein allergy, according to irritated situation difference, has beans based formulas milk, the degree of depthHydrolyzed formula and three kinds of formulas of amino acid formula milk are available. Bovine colostrum infant formula patentCN201010242738.0 has emphasized that the immunoglobulin (Ig) of colostrum own improves infant immunity effect, and examinesConsider FOS and GOS gold combination performance prebiotic function, can not change the allergy of babymilk albumenPhenomenon; Breast base lactose free infant formula powder has solved diarrhoea and the diarrhoea that baby's lactose intolerance causes and has recovered to askTopic, but there is no to solve baby's albumen allergic problem at all. Therefore, albumen allergic problem is these type of childrenIrritated subject matter. Because the irritated infant of part milk protein simultaneously can be to legumin allergy, and beans eggWhite low compared with the bioavilability of milk protein, long-term use may cause growth of weight deficiency, EuropePaediatrics allergy and clinical immunology meeting and U.S.'s pediatric nutrition can not recommend beans based formulas to be applied to milk eggThe prevention of white irritated infant and treatment. The case that is in a bad way or can not makes a definite diagnosis, first-selected depth hydrolysis joinsSide's milk or the treatment of amino acid formula milk. Most infants that suffer from milk protein allergy can tolerate the degree of depthHydrolysis formula powder, if still have allergy sufferers can only select joining using amino acid as nitrogenous source to depth hydrolysis's formulaSide, account for make a definite diagnosis 10%, show as serious enteropathy or multiple food hypersenstivity more. Therefore amino acid formulaFirst-selection as serious systemic anaphylaxis, the serious concurrent Hypoproteinemia of enteropathy or poor growth baby is joinedSide. But pure amino acid formula powder is expensive, and mouthfeel is poor, and has avoided anaphylactogen completely, can not be byStep tolerance, therefore can not serve as primary prevention. Depth hydrolysis's protein prescription powder is by heating, hydrolysis etc.Special process technology, has significantly reduced the antigenicity of protein component and nutritive value and ordinary powdered milk phaseSeemingly, thereby the most conventional.
The initial object of application protein hydrolysate formula is will be by reducing or eliminate the allergenicity of milk proteinThereby reach the object that reduces the irritated risk of milk protein. Join through hydrolysis supervisor treated water solution albumenThe protein of side consists of small peptide or amino acid, in theory compared with intact proteins absorption more easy to digest.
Lactalbumin its antigenicity after being hydrolyzed significantly reduces, and partial function characteristic also improves simultaneously,As foaming characteristic, dissolubility and emulsibility and mouthfeel etc. But owing to making bag in the meeting of hydrolyzed whey protein processBe rolled in the hydrophobic group that intramolecule is bitter taste and come out, the polypeptide that contains more hydrophobic grouping withPeople's taste bud contact just can make people produce bitter taste sensation, applies in product thereby limit it. For baby, molecular weight, lower than dipeptides, the tripeptides of 1000Da, is beneficial to and digests and assimilates most, still, and along with hydrolysisThe increase of degree, bitter taste increases gradually.
Therefore, the lactalbumin hydrolysate of the high debitterize of exploitation degree of hydrolysis be applied to formula powder and make it haveThe irritated effect of effective anti-albumen, will have very important social value.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of lactalbumin depth hydrolysis thing of debitterize,And be applied in a kind of infant formula of anti-albumen allergy.
Another technical problem to be solved by this invention is to provide the infant formula of above-mentioned anti-albumen allergyPreparation method.
For solving the problems of the technologies described above, technical scheme of the present invention is:
An infant formula for anti-albumen allergy, contains lactalbumin hydrolysate, described lactalbumin waterSeparating thing is prepared by following method: every 5.0g concentrated whey protein powder is dissolved in to 95ml distilled waterIn, in 85 DEG C of water-baths, keep after 20min, regulating PH is 7.0, adds trypsase (vigor 25000U/g),Keep 40 DEG C of hydrolysis temperatures, after hydrolysis 4h, 85 DEG C of heating water bath 10min of hydrolyzate enzyme that goes out, centrifugal going sunkForm sediment, regulate PH8.5, add aminopeptidase (vigor 10000U/g), 50 DEG C of hydrolysis temperatures, continue hydrolysis 6h.
Preferably, the infant formula of above-mentioned anti-albumen allergy, by solid glucose syrup, lactalbuminHow unsaturated hydrolysate, mixed vegetable oil, medium chain triglyceride, mineral matter, B B-complex, long-chain beAliphatic acid, emulsifying agent, Choline Chloride, taurine, nucleotides and VBT composition, by its parts by weightMeter solid glucose syrup 30-50 part, lactalbumin hydrolysate 25-40 part, mixed vegetable oil 7-13 part,How unsaturated medium chain triglyceride 12-17 part, mineral matter 3-5 part, B B-complex 0.2-0.7 part, long-chain isAliphatic acid 0.1-0.3 part, emulsifying agent 0.05-0.09 part, Choline Chloride 0.03-0.07 part, taurine 0.02-0.05Part, nucleotides 0.02-0.04 part, VBT 0.005-0.01 part.
Preferably, the infant formula of above-mentioned anti-albumen allergy, by its parts by weight solid glucose sugarStarch 40 parts, 30 parts of lactalbumin hydrolysates, 10 parts of mixed vegetable oils, 15 parts of medium chain triglycerides, ore deposit4.115 parts of materials, 0.5 part of B B-complex, 0.2 part of long-chain polyunsaturated fatty acid, emulsifying agent 0.07Part, 0.05 part of Choline Chloride, 0.03 part of taurine, 0.027 part, nucleotides, 0.008 part of VBT.
Preferably, the infant formula of above-mentioned anti-albumen allergy, described mixed vegetable oil is soybean oil, certain herbaceous plants with big flowersOne or more the mixing of caul-fat, corn oil, palm oil, rapeseed oil; B B-complex be vitamin A,Vitamin D, vitamin E, vitamin K1, Cobastab1, Cobastab2, Cobastab6, vitaminB12, several or whole mixing in nicotinic acid, folic acid, choline, biotin; Mineral matter be calcium, phosphorus,Several or whole mixing in potassium, sodium, chlorine, iron, zinc, copper, manganese, molybdenum, selenium, iodine, chromium; LongChain polyunsaturated fatty acid is arachidonic acid (ARA) and/or DHA (DHA); Emulsifying agent isSoybean lecithin or list, diglycerol fatty acid lemon acid ester; Nucleotides is 5 ' monophosphate cytidine, 5 ' urineThe mixing of one or more in thuja acid disodium, 5 ' AMP, 5 ' uridine monophosphate disodium.
A preparation method for the infant formula of anti-albumen allergy, concrete preparation process is as follows:
(1) mixing of materials
Major ingredient: by formula measure solid glucose syrup, lactalbumin hydrolysate in major ingredient cylinder with 45-55 DEG CPurified water stirring and dissolving;
Small powder: measure mineral matter, Choline Chloride, taurine, nucleotides, VBT respectively specially by formulaWith in small powder cylinder with 45-55 DEG C of pure water stirring and dissolving;
Oil plant: measure mixed vegetable oil, medium chain triglyceride, long-chain polyunsaturated fatty acid by formula and existIn carburetion cylinder, add emulsifiers dissolve;
(2) pasteurize
It is in pasteurization machine at 80-100 DEG C after sterilization 5-10min, cold immediately after major ingredient is mixed with small powderBut to 40-45 DEG C;
(3) homogeneous
The raw material that is preheated to 60-70 DEG C is mixed with oil plant, is under 10-20MPa condition at homogenizer pressureHomogeneous; Cool to 10 DEG C of following keeping in;
(4) Vacuum Concentration
Add purified water to dissolve B B-complex by formula ratio, add in the material after homogeneous, will mixCompound is warming up to concentration and evaporation under 60-70 DEG C of condition;
(5) spraying is dry
After concentrating, material is sprayed and is dried, and drying condition is EAT 150-170 DEG C, logistics flow velocityFor 1.20-1.30L/h, to obtain final product.
Preferably, the preparation method of the infant formula of above-mentioned anti-albumen allergy, breast in described step (1)The preparation method of albumin hydrolysate is: every 5.0g concentrated whey protein powder is dissolved in 95ml distilled water,In 85 DEG C of water-baths, keep after 20min, regulating PH is 7.0, adds trypsase (vigor 25000U/g),Keep 40 DEG C of hydrolysis temperatures, after hydrolysis 4h, 85 DEG C of heating water bath 10min of hydrolyzate enzyme that goes out, centrifugal going sunkForm sediment, regulate PH8.5, add aminopeptidase (vigor 10000U/g), 50 DEG C of hydrolysis temperatures, continue hydrolysis 6h.
The invention has the beneficial effects as follows:
The infant formula of above-mentioned anti-albumen allergy, lactalbumin hydrolysate used is the lactalbumin degree of depthHydrolysate is in specific proportioning situation, lactalbumin to be carried out to Compound Water by trypsase and aminopeptidaseSeparate, make its degree of hydrolysis reach 48-55%, nutritional labeling is without loss, and hydrolysis gained whey protein peptide is easy to digestAbsorb without sensitizing property, eliminated the bitter taste after lactalbumin depth hydrolysis simultaneously, taste is easy to infantAccept;
And by the infant formula of specific prescription, preferably solid glucose syrup, not containing lactose and sucrose,Applicable to lactose intolerance baby, and can promote baby intestinal energy to take in and body weight gain; Fat groupIn one-tenth, substitute long-chain triglyceride with medium chain triglyceride (mediumchaintriglyceride, MCT)(long-chaintriglycerides, LCT), even the in the situation that of pancreatic lipase and the reduction of cholate level, alsoCan absorb fast and effectively, not produce chyle, be applicable to fat digestion, absorption and dysbolism and (compriseChylothorax, chyloperitoneum) baby, this infant formula is particularly applicable to the baby of intestinal dysfunction,Especially there is the baby of disturbance of absorptive function.
Brief description of the drawings
Fig. 1 is that (trypsase is hydrolyzed separately, ammonia for the graph of a relation of different hydrolyzate percent hydrolysis and hydrolysis timeThe independent hydrolysis of peptase and the percent hydrolysis of trypsase-aminopeptidase composite hydrolysis and the comparison of hydrolysis time);
Fig. 2 be graph of a relation between different hydrolyzate bitterness value and hydrolysis time (trypsase is hydrolyzed separately,The independent hydrolysis of aminopeptidase and the bitterness value of trypsase-aminopeptidase composite hydrolysis and the comparison of hydrolysis time).
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is further described.
Embodiment 1
An infant formula for anti-albumen allergy, by solid glucose syrup, lactalbumin hydrolysate,Mixed vegetable oil, medium chain triglyceride, mineral matter, B B-complex, long-chain polyunsaturated fatty acid,Emulsifying agent, Choline Chloride, taurine, nucleotides and VBT composition, wherein, solid glucose syrup40kg, lactalbumin hydrolysate 30kg, mixed vegetable oil 10kg, medium chain triglyceride 15kg, mineral matter4.115kg, B B-complex 0.5kg, long-chain polyunsaturated fatty acid 0.2kg, emulsifying agent 0.07kg, chlorinationCholine 0.05kg, taurine 0.03kg, nucleotides 0.027kg, VBT 0.008kg; Described mixed plantOil is soybean oil and the palm oil oil for mixing of 1:1 composition by volume; Described B B-complex is for by weight466 parts of umber meter vitamin As, 7 parts of vitamin Ds, 8 parts of vitamin Es, vitamin K138 parts, dimensionRaw plain B1372 parts, Cobastab2847 parts, Cobastab6315 parts, Cobastab122.1 parts, nicotinic acid 328The mixture of part, 88 parts, folic acid, 125 parts, choline, 18 parts of compositions of biotin; Described mineral matter is for pressing548 parts of parts by weight meter calcium, 340 parts, phosphorus, 665 parts, potassium, 177 parts, sodium, 392 parts of chlorine, 7.1 parts of iron,The mixture of 4.5 parts, zinc, 316 parts of copper, 17 parts, manganese, 17 parts, selenium, 96 parts of compositions of iodine; Described long-chain is manyUnrighted acid is that arachidonic acid (ARA) and DHA (DHA) form by weight 1:1Mixture; Described emulsifying agent is soybean lecithin; Described nucleotides is 5 ' monophosphate cytidine.
The preparation method of the infant formula of above-mentioned anti-albumen allergy, concrete preparation process is as follows:
(1) mixing of materials
Major ingredient: by formula measure solid glucose syrup, lactalbumin hydrolysate in major ingredient cylinder with 45 DEG CPurified water stirring and dissolving;
Small powder: measure mineral matter, Choline Chloride, taurine, nucleotides, VBT respectively specially by formulaWith in small powder cylinder with 45 DEG C of pure water stirring and dissolving;
Oil plant: measure mixed vegetable oil, medium chain triglyceride, long-chain polyunsaturated fatty acid by formula and existIn carburetion cylinder, add emulsifiers dissolve;
(2) pasteurize
After being mixed with small powder, major ingredient at 100 DEG C after sterilization 5min, is cooled to immediately in pasteurization machine42℃；
(3) homogeneous
The raw material that is preheated to 65 DEG C is mixed with oil plant, is homogeneous under 13MPa condition at homogenizer pressure;Cool to 10 DEG C of following keeping in;
(4) Vacuum Concentration
Add purified water to dissolve B B-complex by formula ratio, add in the material after homogeneous, will mixCompound is warming up to concentration and evaporation under 65 DEG C of conditions;
(5) spraying is dry
After concentrating, material is sprayed and is dried, and drying condition is 152 DEG C of EATs, and logistics flow velocity is1.23L/h, to obtain final product.
Embodiment 2
An infant formula for anti-albumen allergy, by solid glucose syrup, lactalbumin hydrolysate,Mixed vegetable oil, medium chain triglyceride, mineral matter, B B-complex, long-chain polyunsaturated fatty acid,Emulsifying agent, Choline Chloride, taurine, nucleotides and VBT composition, wherein, solid glucose syrup50kg, lactalbumin hydrolysate 25kg, mixed vegetable oil 7kg, medium chain triglyceride 17kg, mineral matter5kg, B B-complex 0.2kg, long-chain polyunsaturated fatty acid 0.1kg, emulsifying agent 0.09kg, Choline Chloride0.07kg, taurine 0.02kg, nucleotides 0.02kg, VBT 0.01kg; Described mixed vegetable oil is soybeanOil and palm oil be the oil for mixing of 1:1 composition by volume; Described B B-complex is for counting by weight dimensionRaw plain A466 part, 7 parts of vitamin Ds, 8 parts of vitamin Es, vitamin K138 parts, Cobastab1372Part, Cobastab2847 parts, Cobastab6315 parts, Cobastab122.1 parts, 328 parts, nicotinic acid, leafThe mixture of 88 parts of acid, 125 parts, choline, 18 parts of compositions of biotin; Described mineral matter is for by weight548 parts of calcium of number meter, 340 parts, phosphorus, 665 parts, potassium, 177 parts, sodium, 392 parts of chlorine, 7.1 parts of iron, zinc 4.5The mixture of part, 316 parts of copper, 17 parts, manganese, 17 parts, selenium, 96 parts of compositions of iodine; The many insatiable hungers of described long-chainTo be arachidonic acid (ARA) and DHA (DHA) by weight 1:1 composition mixed with aliphatic acidCompound; Described emulsifying agent is soybean lecithin; Described nucleotides is 5 ' monophosphate cytidine.
The preparation method of the infant formula of above-mentioned anti-albumen allergy, concrete preparation process is as follows:
(1) mixing of materials
Major ingredient: by formula measure solid glucose syrup, lactalbumin hydrolysate in major ingredient cylinder with 48 DEG CPurified water stirring and dissolving;
Small powder: measure mineral matter, Choline Chloride, taurine, nucleotides, VBT respectively specially by formulaWith in small powder cylinder with 52 DEG C of pure water stirring and dissolving;
Oil plant: measure mixed vegetable oil, medium chain triglyceride, long-chain polyunsaturated fatty acid by formula and existIn carburetion cylinder, add emulsifiers dissolve;
(2) pasteurize
After being mixed with small powder, major ingredient at 80 DEG C after sterilization 8min, is cooled to immediately in pasteurization machine40℃；
(3) homogeneous
The raw material that is preheated to 70 DEG C is mixed with oil plant, is homogeneous under 15MPa condition at homogenizer pressure;Cool to 10 DEG C of following keeping in;
(4) Vacuum Concentration
Add purified water to dissolve B B-complex by formula ratio, add in the material after homogeneous, will mixCompound is warming up to concentration and evaporation under 60 DEG C of conditions;
(5) spraying is dry
After concentrating, material is sprayed and is dried, and drying condition is 160 DEG C of EATs, and logistics flow velocity is1.25L/h, to obtain final product.
Embodiment 3
An infant formula for anti-albumen allergy, by solid glucose syrup, lactalbumin hydrolysate,Mixed vegetable oil, medium chain triglyceride, mineral matter, B B-complex, long-chain polyunsaturated fatty acid,Emulsifying agent, Choline Chloride, taurine, nucleotides and VBT composition, wherein, solid glucose syrup30kg, lactalbumin hydrolysate 40kg, mixed vegetable oil 13kg, medium chain triglyceride 12kg, mineral matter3kg, B B-complex 0.7kg, long-chain polyunsaturated fatty acid 0.3kg, emulsifying agent 0.05kg, Choline Chloride0.03kg, taurine 0.05kg, nucleotides 0.04kg, VBT 0.005kg; Described mixed vegetable oil is largeSoya-bean oil and palm oil be the oil for mixing of 1:1 composition by volume; Described B B-complex is for counting by weight466 parts of vitamin As, 7 parts of vitamin Ds, 8 parts of vitamin Es, vitamin K138 parts, Cobastab1372 parts, Cobastab2847 parts, Cobastab6315 parts, Cobastab122.1 parts, 328 parts, nicotinic acid,The mixture of 88 parts, folic acid, 125 parts, choline, 18 parts of compositions of biotin; Described mineral matter is for by weight548 parts of umber meter calcium, 340 parts, phosphorus, 665 parts, potassium, 177 parts, sodium, 392 parts of chlorine, 7.1 parts of iron, zinc4.5 parts, the mixture of 316 parts of copper, 17 parts, manganese, 17 parts, selenium, 96 parts of compositions of iodine; How not described long-chainSaturated fatty acid is that arachidonic acid (ARA) and DHA (DHA) form by weight 1:1Mixture; Described emulsifying agent is soybean lecithin; Described nucleotides is 5 ' monophosphate cytidine.
The preparation method of the infant formula of above-mentioned anti-albumen allergy, concrete preparation process is as follows:
(1) mixing of materials
Major ingredient: by formula measure solid glucose syrup, lactalbumin hydrolysate in major ingredient cylinder with 50 DEG CPurified water stirring and dissolving;
Small powder: measure mineral matter, Choline Chloride, taurine, nucleotides, VBT respectively specially by formulaWith in small powder cylinder with 48 DEG C of pure water stirring and dissolving;
Oil plant: measure mixed vegetable oil, medium chain triglyceride, long-chain polyunsaturated fatty acid by formula and existIn carburetion cylinder, add emulsifiers dissolve;
(2) pasteurize
After being mixed with small powder, major ingredient at 90 DEG C after sterilization 10min, is cooled to immediately in pasteurization machine40℃；
(3) homogeneous
The raw material that is preheated to 60 DEG C is mixed with oil plant, is homogeneous under 18MPa condition at homogenizer pressure;Cool to 10 DEG C of following keeping in;
(4) Vacuum Concentration
Add purified water to dissolve B B-complex by formula ratio, add in the material after homogeneous, will mixCompound is warming up to concentration and evaporation under 70 DEG C of conditions;
(5) spraying is dry
After concentrating, material is sprayed and is dried, and drying condition is 165 DEG C of EATs, and logistics flow velocity is1.28L/h, to obtain final product.
Embodiment 4
An infant formula for anti-albumen allergy, by solid glucose syrup, lactalbumin hydrolysate,Mixed vegetable oil, medium chain triglyceride, mineral matter, B B-complex, long-chain polyunsaturated fatty acid,Emulsifying agent, Choline Chloride, taurine, nucleotides and VBT composition, wherein, solid glucose syrup38kg, lactalbumin hydrolysate 28kg, mixed vegetable oil 11kg, medium chain triglyceride 16kg, mineral matter4.2kg, B B-complex 0.48kg, long-chain polyunsaturated fatty acid 0.26kg, emulsifying agent 0.07kg, chlorinationCholine 0.06kg, taurine 0.04kg, nucleotides 0.02kg, VBT 0.008kg. Described mixed vegetable oilFor sunflower oil and the corn oil oil for mixing of 1:1 composition by volume; Described B B-complex is for by weight49 parts of vitamin As of number meter, 56 parts of VD1s, 1 part of vitamin E2, vitamin K165 parts, dimensionRaw plain B124 parts, Cobastab251 parts, Cobastab623 parts, Cobastab12104 parts, nicotinic acid 17The mixture of part, 76 parts, folic acid, 25 parts, choline, 17 parts of compositions of biotin; Described mineral matter is heavy for pressing108 parts of amount umber meter calcium, 93 parts, phosphorus, 55 parts, potassium, 59 parts of iron, 46 parts, zinc, 35 parts of copper, selenium 20The mixture of part, 47 parts of compositions of iodine; Described long-chain polyunsaturated fatty acid be arachidonic acid (ARA) andDHA (DHA) is by weight the mixture of 1.5:1 composition; Described emulsifying agent is glycerin fattySour lime acid esters; Described nucleotides is 5 ' AMP.
The preparation method of the infant formula of above-mentioned anti-albumen allergy, concrete preparation process is as follows:
(1) mixing of materials
Major ingredient: by formula measure solid glucose syrup, lactalbumin hydrolysate in major ingredient cylinder with 45 DEG CPurified water stirring and dissolving;
Small powder: measure mineral matter, Choline Chloride, taurine, nucleotides, VBT respectively specially by formulaWith in small powder cylinder with 45 DEG C of pure water stirring and dissolving;
Oil plant: measure mixed vegetable oil, medium chain triglyceride, long-chain polyunsaturated fatty acid by formula and existIn carburetion cylinder, add emulsifiers dissolve;
(2) pasteurize
After being mixed with small powder, major ingredient at 95 DEG C after sterilization 10min, is cooled to immediately in pasteurization machine45℃；
(3) homogeneous
The raw material that is preheated to 65 DEG C is mixed with oil plant, is homogeneous under 20MPa condition at homogenizer pressure;Cool to 10 DEG C of following keeping in;
(4) Vacuum Concentration
Add purified water to dissolve B B-complex by formula ratio, add in the material after homogeneous, will mixCompound is warming up to concentration and evaporation under 65 DEG C of conditions;
(5) spraying is dry
After concentrating, material is sprayed and is dried, and drying condition is 170 DEG C of EATs, and logistics flow velocity is1.30L/h, to obtain final product.
The described lactalbumin hydrolysate of one of embodiment 1-4 is prepared by following method: by every 5.0gConcentrated whey protein powder is dissolved in 95ml distilled water, in 85 DEG C of water-baths, keeps after 20min, regulates PHBe 7.0, add trypsase (vigor 25000U/g), keep 40 DEG C of hydrolysis temperatures, after hydrolysis 4h, waterSeparate liquid 85 DEG C of heating water bath 10min enzyme that goes out, the centrifugal precipitation of going, regulates PH8.5, adds aminopeptidase (vigor10000U/g), 50 DEG C of hydrolysis temperatures, continue hydrolysis 6h, after being hydrolyzed, and the rapid water-bath liter of hydrolyzateTemperature, to 90 DEG C, maintains 15min inactivator. Hydrolyzate, in the centrifugal 20min of 4000r/min, is discarded to precipitation,Supernatant is the lactalbumin depth hydrolysis thing of debitterize; Other components are commercially available prod, and each component is usedAmount increases or reduces according to same ratio, and the parts by weight relation of the each component of gained all belongs to guarantor of the present inventionProtect scope.
Test case 1
Trypsase is hydrolyzed separately, aminopeptidase is hydrolyzed separately and the hydrolysis of trypsase-aminopeptidase composite hydrolysisThe comparison of rate, bitterness value and hydrolysis time
Method of testing:
Trypsase is hydrolyzed separately: every 5.0g concentrated whey protein powder is dissolved in 95ml distilled water to 85 DEG CIn water-bath, keep after 20min, regulating PH is 7.0, adds trypsase (vigor 25000U/g), protectsHold 40 DEG C of hydrolysis temperatures, hydrolysis 10h. In hydrolytic process, get a certain amount of hydrolyzate every 2h, for surveyingDetermine proteolysis rate and bitter taste score value;
Aminopeptidase is hydrolyzed separately: every 5.0g concentrated whey protein powder is dissolved in 95ml distilled water to 85 DEG CIn water-bath, keep after 20min, regulate PH8.5, add aminopeptidase (vigor 10000U/g), hydrolysis temperature50 DEG C, hydrolysis 10h. In hydrolytic process, get a certain amount of hydrolyzate every 2h, for measuring proteolysisRate and bitter taste score value;
Complex enzyme for hydrolyzing: every 5.0g concentrated whey protein powder is dissolved in 95ml distilled water to 85 DEG C of water-bathsAfter middle maintenance 20min, regulating PH is 7.0, adds trypsase (vigor 25000U/g), keeps waterSeparate 40 DEG C of temperature, after hydrolysis 4h, 85 DEG C of heating water bath 10min of hydrolyzate enzyme that goes out, the centrifugal precipitation of going, adjustsJoint PH8.5, adds aminopeptidase (vigor 10000U/g), and 50 DEG C of hydrolysis temperatures continue hydrolysis 6h. HydrolysisIn process, get a certain amount of hydrolyzate every 2h, for measuring proteolysis rate and bitter taste score value.
The mensuration of percent hydrolysis: adopt formol titration.
Bitter taste estimate: take 2g Ilex Latifolia Thunb, add 500ml distilled water and boil 30min, filter, be settled to 500ml.Dilute respectively 20,40,60,80,100 times, assess with 10 powder systems, bitter taste score value is little for evaluatingThe average of group (10 people). Standards of grading: without bitter taste: 2; Micro-hardship: 4; More bitter: 6; Bitter: 8;Very bitter: 10.
As shown in Figure 1 and Figure 2, under the same hydrolysis time, single use trypsase and use complex enzyme waterThe hydrolysis degree no significant difference of separating, and use separately aminopeptidase percent hydrolysis lower; The hydrolysis of complex enzyme hydrolysisLiquid bitter taste score value is starkly lower than the hydrolyzate of single use trypsin hydrolysis; Consider hydrolysis time,The factors such as hydrolysis degree and hydrolyzate bitter taste, optimize hydrolysising condition, adopt the trypsase-ammonia of special ratiosPeptase composite hydrolysis lactalbumin can obtain low bitter taste depth hydrolysis's hydrolysate.
Test case 2
The observation of curative effect of depth hydrolysis's protein prescription powder to milk allergy infant gastrointestinal function
Test crowd: select to intend being diagnosed as milk allergy infant 30 examples. All meeting diarrhea disease of inpatient children in 2009 examinesThe diagnostic criteria of disconnected principle of reatment and the hypersensitive diagnosis of infant's milk protein and treatment guide. This group 30In example infant, male 16 examples, female's 14 examples; Age 2-6 month, average (4.2 ± 1.2) individual month; Course of disease 3d-2Individual month. Infant gastrointestinal symptom shows in table 1.
Methods for the treatment of: the ordinary powdered milk of stopping using, use the baby of anti-albumen allergy described in the embodiment of the present invention 1 instead and joinSide's powder is fed, and the employing of mixed feeding infant stops breast milk breast-feeds, and simple depth hydrolysis's protein prescription powder, feedsTime is 2 weeks. After 2 weeks, can try to feed ordinary powdered milk, initial amount is that 1/4 ordinary powdered milk mixes 3/4 depth hydrolysis's eggWhite formula powder, observes stool proterties 2-3d; Stool normally increases by 1/4 then again, and 1/2 ordinary powdered milk mixes1/2 depth hydrolysis's protein prescription powder, observes stool proterties 2-3d; Stool proterties is extremely changeed back embodiment 1The infant formula of described anti-albumen allergy is fed; As follows analogize each increase by 1/4 ordinary powdered milk, until mistakeDegree is to 100% ordinary powdered milk. Medical worker carries out Effect of follow-up visit by telephone gastrointestinal symptom for 1 week, 2 weeks and 4 weeks in nursing.And record and compare infant treatment before and the treatment 4 weeks variation of the indexs such as periphery immunoglobulin (Ig)s afterwards. Statistics knotFruit is in table 2 and table 3.
Evaluation index: observe and record infant result for the treatment of. Treatment results is effective: symptom after infant treatmentIn one week, significantly take a turn for the better; Effective: after infant treatment, in two weeks, symptom takes a turn for the better; Invalid: infant treatmentAfter in surrounding symptom without significant change.
Statistical method: each variable represents with x ± s. Adopt t inspection, P < 0.05 will be considered to checked poorThere is not statistical significance.
Conclusion: adopt the formula of the lactalbumin hydrolysate that contains depth hydrolysis, and utilize solid glucoseThe particular combinations of syrup, medium chain triglyceride etc., is hydrolyzed to little peptide and amino acid by high molecular weight protein, hasEffect has reduced modification structure and the steric configuration of high molecular weight protein, has eliminated the sensibiligen in milk, effectivelyAlleviate infant gastrointestinal symptom; After infant treatment periphery Immunoglobulin IgE level treat front obviously underSignificant difference (P < 0.01) falls. Further confirmed Immunoglobulin IgE plays in babymilk allergyThe clinical effective property of important effect and this formula powder.
The routine gastrointestinal symptom performance of table 1 milk allergy infant 30 (example, %)
Table 2 milk allergy infant 30 routine results for the treatment of
Before and after the treatment of table 3 infant, periphery amynologic index changes
It is above-mentioned that with reference to embodiment, infant formula to this kind of anti-albumen allergy and preparation method thereof carries outDescribing in detail, is illustrative instead of determinate, can list several in fact according to institute's limited rangeExecute example, therefore in the variation and the amendment that do not depart under general plotting of the present invention, should belong to protection model of the present inventionWithin enclosing.
Claims (4)
1. an infant formula for anti-albumen allergy, contains lactalbumin hydrolysate, its spyLevy and be: described lactalbumin hydrolysate is prepared by following method: by every 5.0gConcentrated whey protein powder is dissolved in 95ml distilled water, in 85 DEG C of water-baths, keeps after 20min,Regulating pH is 7.0, adds trypsase 25000U/g, keeps 40 DEG C of hydrolysis temperatures, hydrolysisAfter 4h, 85 DEG C of heating water bath 10min of hydrolyzate enzyme that goes out, the centrifugal precipitation of going, regulates pH8.5,Add aminopeptidase 10000U/g, 50 DEG C of hydrolysis temperatures, continue hydrolysis 6h.
2. the infant formula of anti-albumen allergy according to claim 1, its feature existsIn: planted by lactalbumin hydrolysate described in solid glucose syrup, claim 1, mixingThing oil, medium chain triglyceride, mineral matter, B B-complex, long-chain polyunsaturated fatty acid,Emulsifying agent, Choline Chloride, taurine, nucleotides and VBT composition, by its parts by weightMeter solid glucose syrup 30-50 part, lactalbumin hydrolysate 25-40 part, mixed vegetable oil7-13 part, medium chain triglyceride 12-17 part, mineral matter 3-5 part, B B-complex 0.2-0.7Part, long-chain polyunsaturated fatty acid 0.1-0.3 part, emulsifying agent 0.05-0.09 part, chlorination courageAlkali 0.03-0.07 part, taurine 0.02-0.05 part, nucleotides 0.02-0.04 part, L-Carnitine 0.005-0.01 part, wherein, described mixed vegetable oil is soybean oil, sunflower oil, jadeOne or more the mixing of rice bran oil, palm oil, rapeseed oil; B B-complex be vitamin A,Vitamin D, vitamin E, vitamin K1, Cobastab1, Cobastab2, Cobastab6、Cobastab12, several or whole mixing in nicotinic acid, folic acid, choline, biotin; Ore depositMaterial is in calcium, phosphorus, potassium, sodium, chlorine, iron, zinc, copper, manganese, molybdenum, selenium, iodine, chromiumSeveral or whole mixing; Long-chain polyunsaturated fatty acid is arachidonic acid and/or 22Carbon acid; Emulsifying agent is soybean lecithin or list, diglycerol fatty acid lemon acid ester; NucleosidesAcid is 5 ' monophosphate cytidine, 5 ' uridine monophosphate disodium, 5 ' AMP, 5 ' uridylic acidThe mixing of one or more in disodium.
3. the infant formula of anti-albumen allergy according to claim 2, its feature existsIn: by its 40 parts, parts by weight solid glucose syrup, 30 parts of lactalbumin hydrolysates,10 parts of mixed vegetable oils, 15 parts of medium chain triglycerides, 4.115 parts, mineral matter, compound supporting one's family0.5 part of element, 0.2 part of long-chain polyunsaturated fatty acid, 0.07 part of emulsifying agent, Choline Chloride0.05 part, 0.03 part of taurine, 0.027 part, nucleotides, 0.008 part of VBT.
4. the preparation method of the infant formula of anti-albumen allergy described in claim 2, its spyLevy and be: concrete preparation process is as follows:
(1) mixing of materials
Major ingredient: measure solid glucose syrup, lactalbumin hydrolysate in major ingredient cylinder by formulaWith 45-55 DEG C of purified water stirring and dissolving;
Small powder: measure mineral matter, Choline Chloride, taurine, nucleotides, VBT by formulaRespectively in special small powder cylinder with 45-55 DEG C of pure water stirring and dissolving;
Oil plant: measure mixed vegetable oil, medium chain triglyceride, the many unsaturated lipids of long-chain by formulaFat acid adds emulsifiers dissolve in carburetion cylinder;
(2) pasteurize
After major ingredient is mixed with small powder in pasteurization machine sterilization 5-10min at 80-100 DEG CAfter, be cooled to immediately 40-45 DEG C;
(3) homogeneous
The raw material that is preheated to 60-70 DEG C is mixed with oil plant, is 10-20MPa at homogenizer pressureHomogeneous under condition; Cool to 10 DEG C of following keeping in;
(4) Vacuum Concentration
Add purified water to dissolve B B-complex by formula ratio, add in the material after homogeneous,Mixture is warming up to concentration and evaporation under 60-70 DEG C of condition;
(5) spraying is dry
After concentrating, material is sprayed and is dried, and drying condition is EAT 150-170 DEG C,Logistics flow velocity is 1.20-1.30L/h, to obtain final product.
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| CN110037115A (en) * | 2019-05-14 | 2019-07-23 | 宁波特壹食品有限公司 | A kind of partially hydrolysed protein formula powder and preparation method thereof |
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| CN114007435A (en) * | 2019-06-13 | 2022-02-01 | N·V·努特里奇亚 | Method of making an infant formula containing hydrolyzed protein |
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