CN104020149A - Establishment method for echinogorgia pseudossapo laser-Raman spectrum - Google Patents
Establishment method for echinogorgia pseudossapo laser-Raman spectrum Download PDFInfo
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- CN104020149A CN104020149A CN201310224796.4A CN201310224796A CN104020149A CN 104020149 A CN104020149 A CN 104020149A CN 201310224796 A CN201310224796 A CN 201310224796A CN 104020149 A CN104020149 A CN 104020149A
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- pseudossapo
- echinogorgia
- gorgonian
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Abstract
The invention relates to an establishment method for an echinogorgia pseudossapo laser-Raman spectrum. The establishment method for the echinogorgia pseudossapo laser-Raman spectrum comprises the following steps: (1) preparing a test sample: taking an echinogorgia pseudossapo sample and cutting into strip-shaped grains, filling the grains into a sample bottle to be quickly frozen, drying the quickly-frozen sample by using a vacuum freeze drying machine and grinding the sample into powder, and filtering the powder by using a screening net to obtain a powdery echinogorgia pseudossapo sample; (b) putting a buffering solution containing the particle-shaped echinogorgia pseudossapo into a focal point by using a focusing laser beam, and exciting Raman scattering of the caught echinogorgia pseudossapo to obtain the laser-Raman spectrum of the test sample. Compared with an existing echinogorgia pseudossapo analysis and identification technology, the identification method is a pure optical method, needs few sample quantity, has short testing time and has an accurate testing result; the sample is not damaged in a testing process and chemical pollutants are not generated; the established laser-Raman spectrum can be used for rapidly and effectively identifying the echinogorgia pseudossapo sample and provides an effective qualitative identification standard to deep researches.
Description
Technical field
The invention belongs to gorgonian and identify field, be specifically designed into a kind of laser Raman spectroscopy that utilizes sparse branching thorn gorgonian is carried out to qualitative mirror method for distinguishing.
Background technology
Raman spectroscopy is by irradiating object with exciting light, collects its interior molecules the scattering spectrum of exciting light is analyzed, and can obtain molecular vibration, rotation aspect information, and is applied to judge a kind of method of the interior molecular structure of object and content.Light tweezer is to utilize the high laser beam imprison object converging, to reach the effect being fixed in solution.Laser tweezers Raman spectrum in conjunction with optical tweezer technology is called laser tweezers Raman spectrum (LTRS), and it makes object more approach its natural physiological situation, and the uncertainty having produced while having reduced object motion, has reduced the interference of veiling glare, has improved signal to noise ratio (S/N ratio).In recent years, Raman spectroscopy has been widely used in biological field, as disease judgement, microbial identification, the many aspects such as cell imaging.
Gorgonian (Gorgonian) belongs to Coelenterata Actinozoa Alcyonaria soft coral order, is the general designation of three kinds of suborders such as hard axle coral, holoaxial coral and calcium axle coral.Gorgonian individuality is supported by axis, and profile is main mainly with fan-shaped, branch shape, whip shape etc., and the whole world approximately has 1200 kinds.The research of gorgonian relates to the many aspects such as taxonomy, chemistry, ecology.Yet the raman spectral characteristics of gorgonian has no bibliographical information always.The present invention adopts laser Raman spectroscopy method to carry out qualitative detection to sparse branching thorn gorgonian, and method is easy, and analysis speed is fast.At present, not yet find the laser raman research report that gorgonian is relevant.
Summary of the invention
It is a kind of easy and simple to handle that the object of the invention is to provide, rapidly the laser raman detection method of Qualitative Identification sparse branching thorn gorgonian.Technical scheme of the present invention is as follows:
(1) sample preparation: sparse branching thorn gorgonian sample is cut into inch strips after blocky-shaped particle, packs sample bottle into and carries out quick-frozen, terminal temperature approximately-10-100 ℃.Shift that quick-frozen sample is in the hothouse of vacuum freeze drier, vacuum tightness is 0.01-0.1MPa, and be 12-48h drying time.Dried sparse branching thorn gorgonian sample is milled into after powder with agate mortar, then filters with 100-400 mesh filter screen, obtains Powdered sparse branching thorn gorgonian sample.
(2) spectral conditions: instrument laser power 10-100mW, sweep time 5-20s.By a branch of wavelength, be that 500-1500nm laser imports an inverted biologic microscope after filtering afterwards.Laser beam has formed a single beam photo potential trap after oil immersion objective (aperture is 1.00-3.00, and enlargement factor is 50-300 times) focuses near focus.Sample cell has sealed the quartz cover slide of 20-200 μ m by going to the bottom and glass plate that top has sealed cover glass forms, and there is the hole that a diameter is 1-10mm at glass plate center.
(3) spectroscopic assay: be placed in glass plate center pit in the damping fluid that contains sparse branching thorn gorgonian sample particulate, the radiation pressure imprison that the laser beam that is focused produces is near focus, and this Shu Jiguang is also used for exciting the Raman scattering of sparse branching thorn gorgonian sample of being held in captivity simultaneously.Before measuring sparse branching thorn gorgonian sample, with the spherical particle of polystyrene that is suspended in the diameter 1-10 μ m in water, proofread and correct.
(4) characteristic spectrum: by actual scanning result and the calculated results contrast, proofread, point out the vibration principle of each spectrum peak in laser raman collection of illustrative plates, determine that the principal character peak scope of sparse branching thorn gorgonian is 1000-1050cm
-1, 1100-1200cm
-1, 1450-1550cm
-1.
Advantage of the present invention: adopt laser Raman spectroscopy of the present invention to carry out qualitative detection to sparse branching thorn gorgonian, easy and simple to handle, quick and precisely qualitative analysis, can be used for the fast detecting of extensive sample.
Accompanying drawing explanation
The laser Raman spectroscopy of Fig. 1 sparse branching thorn gorgonian.
Embodiment
Below in conjunction with embodiment, the present invention is described in further details, but the present invention is not limited only to following enforcement.
Sparse branching thorn gorgonian sample 0.10g cuts into inch strips after blocky-shaped particle, packs sample bottle into and carries out quick-frozen, approximately-80 ℃ of terminal temperatures.Shift that quick-frozen sample is in the hothouse of vacuum freeze drier, vacuum tightness is 0.08MPa, and be 36h drying time.Dried gorgonian sample is milled into after powder with agate mortar, then filters with 100 mesh filter screens, obtains sparse branching thorn gorgonian powdered samples.
By a branch of wavelength, be that 780nm laser imports a Nikon inverted biologic microscope (TE2000U, Nikon) after filtering afterwards.Laser beam has formed a single beam photo potential trap after oil immersion objective (aperture is 1.30, and enlargement factor is 100 times) focuses near focus.Sample cell has sealed the quartz cover slide of 100 μ m by going to the bottom and glass plate that top has sealed No. 1 cover glass forms, and there is the hole that a diameter is 6mm at glass plate center.In the damping fluid that contains sparse branching thorn gorgonian sample particulate, be placed in glass plate center pit, the radiation pressure imprison that the laser beam being focused produces is near focus, and this Shu Jiguang is also used for exciting the Raman scattering of sparse branching thorn gorgonian sample of being held in captivity simultaneously.Before measuring sparse branching thorn gorgonian sample, with the spherical particle of polystyrene that is suspended in the diameter 2 μ m in water, proofread and correct.
The laser Raman spectroscopy of sparse branching thorn gorgonian sample 1006,1111,1501cm-1 bands of a spectrum overshooting goes out.Wherein, 1006cm
-1peak representative-CH
3horizontal rocking vibration pattern, 1111cm
-1peak represents C-C stretching vibration pattern, 1501cm
-1peak represents C=C stretching vibration pattern.
Above preferred embodiment is only for illustrating content of the present invention; in addition; the present invention also has other embodiments; in every case those skilled in the art are because of technology enlightenment involved in the present invention, and adopt to be equal to, replace or technical scheme that equivalent deformation mode forms all drops in protection scope of the present invention.
Claims (1)
1. a method for building up for sparse branching thorn gorgonian laser raman collection of illustrative plates, is characterized in that adopting laser Raman spectrometry, specifically comprises the steps:
(a) sample preparation: sparse branching thorn gorgonian sample is cut into inch strips after blocky-shaped particle, packs sample bottle into and carries out quick-frozen, terminal temperature approximately-10-100 ℃.Shift that quick-frozen sample is in the hothouse of vacuum freeze drier, vacuum tightness is 0.01-0.1MPa, and be 12-48h drying time.Dried sparse branching thorn gorgonian sample is milled into after powder with agate mortar, then filters with 100-400 mesh filter screen, obtains Powdered sparse branching thorn gorgonian sample.
(b) spectral conditions: be that 500-1500nm laser imports an inverted biologic microscope after filtering afterwards by a branch of wavelength.Laser beam has formed a single beam photo potential trap after oil immersion objective (aperture is 1.00-3.00, and enlargement factor is 50-300 times) focuses near focus.Sample cell has sealed the quartz cover slide of 20-200 μ m by going to the bottom and glass plate that top has sealed cover glass forms, and there is the hole that a diameter is 1-10mm at glass plate center.
(c) spectroscopic assay: be placed in glass plate center pit in the damping fluid that contains sparse branching thorn gorgonian sample particulate, the radiation pressure imprison that the laser beam that is focused produces is near focus, and this Shu Jiguang is also used for exciting the Raman scattering of sparse branching thorn gorgonian sample of being held in captivity simultaneously.Before measuring sparse branching thorn gorgonian sample, with the spherical particle of polystyrene that is suspended in the diameter 1-10 μ m in water, proofread and correct.
(d) characteristic spectrum: by actual scanning result and the calculated results contrast, proofread, point out the vibration principle of each spectrum peak in laser raman collection of illustrative plates, determine that the principal character peak scope of sparse branching thorn gorgonian is 1000-1050cm
-1, 1100-1200cm
-1, 1450-1550cm
-1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310224796.4A CN104020149A (en) | 2013-06-07 | 2013-06-07 | Establishment method for echinogorgia pseudossapo laser-Raman spectrum |
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| CN201310224796.4A CN104020149A (en) | 2013-06-07 | 2013-06-07 | Establishment method for echinogorgia pseudossapo laser-Raman spectrum |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105606586A (en) * | 2015-12-23 | 2016-05-25 | 东南大学 | High-stability SERS (surface-enhanced Raman scattering) liquid-phase detection device and detection method |
| CN105891131A (en) * | 2016-04-05 | 2016-08-24 | 中国科学院南海海洋研究所 | Shore-based hermatypic coral spectral measurement method |
| CN109580494A (en) * | 2018-11-21 | 2019-04-05 | 深圳达闼科技控股有限公司 | A kind of detection method, relevant apparatus and storage medium |
| CN109900531A (en) * | 2018-12-29 | 2019-06-18 | 海南大学 | A kind of extracting method of coral lipid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103398999A (en) * | 2013-08-08 | 2013-11-20 | 浙江大学 | Detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy |
-
2013
- 2013-06-07 CN CN201310224796.4A patent/CN104020149A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103398999A (en) * | 2013-08-08 | 2013-11-20 | 浙江大学 | Detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy |
Non-Patent Citations (3)
| Title |
|---|
| CHAD L. LEVERETTE ET AL.: "Determination of carotenoid as the purple pigment in Gorgonia ventalina sclerites using Raman microscopy", 《SPECTROCHIMICA ACTA PART A》 * |
| LENIZE F. MAIA ET AL.: "Colour diversification in octocorals based on conjugated polyenes: A Raman spectroscopic view", 《JOURNAL OF RAMAN SPECTROSCOPY》 * |
| LENIZE F. MAIA ET AL.: "Polyenic pigments from the Brazilian octocoral Phyllogorgia dilatata Esper, 1806 characterized by Raman spectroscopy", 《JOURNAL OF RAMAN SPECTROSCOPY》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105606586A (en) * | 2015-12-23 | 2016-05-25 | 东南大学 | High-stability SERS (surface-enhanced Raman scattering) liquid-phase detection device and detection method |
| CN105891131A (en) * | 2016-04-05 | 2016-08-24 | 中国科学院南海海洋研究所 | Shore-based hermatypic coral spectral measurement method |
| CN109580494A (en) * | 2018-11-21 | 2019-04-05 | 深圳达闼科技控股有限公司 | A kind of detection method, relevant apparatus and storage medium |
| CN109580494B (en) * | 2018-11-21 | 2021-09-28 | 深圳达闼科技控股有限公司 | Detection method, related device and storage medium |
| CN109900531A (en) * | 2018-12-29 | 2019-06-18 | 海南大学 | A kind of extracting method of coral lipid |
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Application publication date: 20140903 |