CN105891131A - Shore-based hermatypic coral spectral measurement method - Google Patents
Shore-based hermatypic coral spectral measurement method Download PDFInfo
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- CN105891131A CN105891131A CN201610211499.XA CN201610211499A CN105891131A CN 105891131 A CN105891131 A CN 105891131A CN 201610211499 A CN201610211499 A CN 201610211499A CN 105891131 A CN105891131 A CN 105891131A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
- G01N2021/3148—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths using three or more wavelengths
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Abstract
The invention discloses a shore-based hermatypic coral spectral measurement method. A black adiaphanous cylinder is arranged, a water inlet is formed in the lower end of the cylinder, a water outlet is formed in the upper end of the cylinder, a black iron stand table is placed in the cylinder, sea water enters the cylinder through the water inlet and overflows through the water outlet, the sea water is kept flowing, then a hermatypic coral sample block is placed in the iron stand table in the cylinder containing the sea water, then a light source irradiates the hermatypic coral sample block, and a spectrograph is adopted for measuring the spectral reflectance of the hermatypic coral sample block; in the measuring process, a spectrograph probe is perpendicular to the surface of the hermatypic coral sample block, measurement is carried out many times, and averaging is carried out. Based on a shore-based laboratory, the wild measuring environment is simulated in the shore-based laboratory, and the spectral reflectance of hermatypic coral is measured. Therefore, a large amount of field observation time can be saved, measurement can be completed simply by taking back part of living samples in a hermatypic coral area, hard to reach, in a remote region, and the time and economic cost of field survey are greatly saved.
Description
Technical field:
The invention belongs to the spectral reflectivity fields of measurement of project cost consulting item sample, be specifically related to a kind of bank base project cost consulting item light
Spectrometry method.
Background technology:
Coral reef ecologic system has very important ecology service, society, economy and cultureal value.But, seawater surface
The factor changes such as temperature rising, Ocean acidification, seawater pollution and mankind's activity aggravation directly influence coral reef health status,
Global coral ecosystem by destroy extensive coral bleaching death incident frequency existing.Remote sensing technology has in real time, large area
Monitoring characteristic, it is possible to promote monitoring and the management of coral reef ecologic system.Carry out coral reef substrate spectral reflectivity feature identification
It is the difficult point of coral reef remote sensing technology, is also the focus of current research simultaneously.Implement coral reef ecologic system comprehensively, supervise in real time
Survey and protection, coral reef ecological nursing is had very important significance.Traditional method cannot obtain large-area observation
Data, are especially difficult to understand remote districts coral reef environmental health situation.Remote sensing technology has the features such as comprehensive, real-time monitoring,
The demand of coral reef ecologic system monitoring can be met.The basis of Remote Sensing Study is to enter according to different coral reef substrate spectral signatures
Row inverting, contrast inversion result and field data research coral reef substrate situation.
Summary of the invention:
Present invention aim to address that existing method of testing cannot obtain the limitation of coverage count data, and be difficult to understand partially
The technical problem of remote area coral reef environmental health situation, and a kind of time that can be greatly saved field investigation and economy are provided
The bank base project cost consulting item spectral measurement method of cost.
The bank base project cost consulting item spectral measurement method of the present invention, it is characterised in that comprise the following steps:
There is the cylinder body of black non transparent, be provided with water inlet in the lower end of cylinder body, upper end is provided with outlet, place in cylinder body
The iron stand of black, sea water enters from water inlet, and outlet overflows, and keeps sea water flowing, then by project cost consulting item sample
Block is put on the iron stand in the cylinder body filling sea water, then uses light source to irradiate project cost consulting item sample blocks, and uses spectrum
The spectral reflectivity of project cost consulting item sample blocks measured by instrument, by spectrogrph probe vertical in project cost consulting item sample blocks table during measurement
Face, repetitive measurement is averaged.
Preferably, the intensity of illumination of described light source is 106μmol/m2/s.Further, described light source includes that wavelength is 400~700nm
Visible ray.
Preferably, described holding sea water flowing, its ocean temperature is 26 DEG C~28 DEG C, and its seawater velocity is 0.5L/min
Preferably, the cylinder body of described black non transparent is to paste black wallpaper, diapire laying covering black nylon cloth at inwall
Glass jar, the iron stand of described black is the iron stand of black electrical adhesive tape looping
Preferably, the described spectral reflectivity using spectrometer measurement project cost consulting item sample blocks, wavelength interval when it is measured
It is 0.5~1nm, and smooth 3 times.
Preferably, the described spectral reflectivity using spectrometer measurement project cost consulting item sample blocks, during measurement, spectrogrph is popped one's head in
It is perpendicular to project cost consulting item sample blocks surface, when being 25 ° when using the spectrogrph probe angle of visual field, project cost consulting item sample blocks straight
Footpath >=50mm, or when being the full visual field when tested, the diameter >=100mm of project cost consulting item sample blocks, described spectrogrph probe
It is 5cm with the distance of project cost consulting item sample blocks.
The present invention utilizes the feature of field Experiments of Optics, is designed to a set of maturation method with regard to coral reef substrate spectral measurement.Pass through
Setting special device is under conditions of having permanent current sea water, by bank base laboratory simulation field condition project cost consulting item spectral reflectance
Rate is measured, thus obtains a large amount of all kinds of project cost consulting item spectral reflectivity information, for further research and protection work from now on
Lay theoretical basis and realistic basis.
The invention has the beneficial effects as follows: in currently available technology, field measurement project cost consulting item spectral reflectivity not only needs in a large number
Manpower and materials, and time-consuming the longest, additionally, be especially difficult to be fully understood by remote districts coral reef health status.This
Bright with bank base laboratory for relying on, test lab simulation field measurement environment, measurement project cost consulting item spectral reflectance by bank base
Rate.So can save the substantial amounts of field inspection time, and remote districts etc. are difficult to the project cost consulting item district arrived,
As long as taking back part biopsy sample, can complete to measure, being greatly saved time and the Financial cost of field investigation.
The present invention can be used for the spectral reflectivity bank base laboratory collecting work of project cost consulting item sample.
Accompanying drawing illustrates:
Fig. 1 is the photo of the project cost consulting item sample blocks of embodiment 1;
Fig. 2 is the spectral reflectivity figure of the project cost consulting item sample blocks of embodiment 1;
Fig. 3 is the photo of the project cost consulting item sample blocks of embodiment 2;
Fig. 4 is the spectral reflectivity figure of the project cost consulting item sample blocks of embodiment 2;
Fig. 5 is present invention structural representation when measuring;
Wherein 1, glass jar;2, water inlet;3, outlet;4, iron stand;5, light source;6, spectrogrph;7, spectrogrph is visited
Head;8, project cost consulting item sample blocks.
Detailed description of the invention:
Following example are to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1:
The bank base project cost consulting item spectral measurement method of the present embodiment, specifically follows the steps below:
One as it is shown in figure 5, glass jar 1 inwall paste black wallpaper, and in glass jar lay black nylon cloth,
The lower end of glass jar is provided with water inlet 2, upper end is provided with outlet 3, places iron stand 4 in cylinder body, and iron stand is in advance with black
The electrical adhesive tape compact winding of color, sea water is passed through by water inlet, outlet flows out, and is then put by project cost consulting item sample blocks 8
On the iron stand of the glass jar entering to fill sea water, control seawater velocity is 0.5L/min, is passed through ocean temperature and is 26-28 DEG C, protects
Hold sea water permanent current;
Two, using light intensity is 106μmol/m2Project cost consulting item sample blocks in light source 5 irradiating step one glass jar of/s,
And use spectrogrph 6 to measure the spectral reflectivity of project cost consulting item sample blocks, it is perpendicular to make reef by spectrogrph probe 7 during measurement
Cave coral sample blocks surface, spectrogrph probe 7 is 5cm with the distance of project cost consulting item sample blocks 8, and 10 measurements are averaged
Value, completes bank base Corallium Japonicum Kishinouye spectral measurement method.
Project cost consulting item sample blocks in the present embodiment step one is block Corallium Japonicum Kishinouye, a diameter of 60mm, the collection of project cost consulting item
Place is China marine site, Sanya Coral Reefs of Luhuitou, Hainan;
In step 2, the spectrum of radiation source includes the visible ray that wavelength is 400~700nm, and it is the most electric that pre-test measured by each sample
Stream, the spectrogrph of employing is U.S.'s marine optics spectrogrph (USB2000+), and it measures scope 200nm-850nm, differentiates
Rate 1.5nm, spectrum sample interval 0.6nm, and smooth 3 times, the probe angle of visual field is 25 °.
The photo of the project cost consulting item sample blocks of the present embodiment is as shown in Figure 1;
The spectral reflectivity figure of the present embodiment project cost consulting item sample blocks is as shown in Figure 2.
Embodiment 2:
The bank base Corallium Japonicum Kishinouye spectral measurement method of the present embodiment, specifically follows the steps below:
One as it is shown in figure 5, glass jar 1 inwall paste black wallpaper, and in glass jar lay black nylon cloth,
The lower end of glass jar is provided with water inlet 2, upper end is provided with outlet 3, places iron stand 4 in cylinder body, and iron stand is in advance with black
The electrical adhesive tape compact winding of color, sea water is passed through by water inlet, outlet flows out, and is then put by project cost consulting item sample blocks 8
Entering to fill on the iron stand 4 in the glass jar of sea water, control seawater velocity is 0.5L/min, and being passed through ocean temperature is 26 DEG C;
Two, using light intensity is 106μmol/m2Project cost consulting item sample blocks in light source 5 irradiating step one glass jar of/s,
And use spectrogrph 6 to measure the spectral reflectivity of project cost consulting item sample blocks, it is perpendicular to make reef by spectrogrph probe 7 during measurement
Cave coral sample blocks surface, spectrogrph probe 7 is 5cm with the distance of project cost consulting item sample blocks 8, and 10 measurements are averaged
Value, completes bank base Corallium Japonicum Kishinouye spectral measurement method.
Project cost consulting item sample blocks in the present embodiment step one is tree coral, a diameter of 65mm, the collection of project cost consulting item
Place is China The Deer Turning lts Head marine site, Sanya;
In step 2, the spectrum of radiation source includes the visible ray that wavelength is 400~700nm, and it is the most electric that pre-test measured by each sample
Stream, the spectrogrph of employing is U.S.'s marine optics spectrogrph (USB2000+), and it measures scope 200nm-850nm, differentiates
Rate 1.5nm, spectrum sample interval 0.6nm, and smooth 3 times, the probe angle of visual field is 25 °.
The photo of the project cost consulting item sample blocks of the present embodiment is as shown in Figure 3;
The spectral reflectivity figure of the project cost consulting item sample blocks of the present embodiment is as shown in Figure 4.
By the block Corallium Japonicum Kishinouye that The Deer Turning lts Head marine site, Sanya is gathered and tree coral, the spectral reflectance of above-described embodiment 1 and 2
Rate gathers, and is analyzed its spectral reflectivity in visible light wave range, and result shows, block Corallium Japonicum Kishinouye and tree coral
Spectral reflectivity changes similar on waveform, 3 crest features and the Hochberg report of Corallium Japonicum Kishinouye spectral reflectivity all occurs
Field measurement acquired results consistent (Hochberg et al, 2000), also occurs in that equally owing to chlorophyll is inhaled at 675nm
The trough received, but block Corallium Japonicum Kishinouye spectral reflectivity is higher than tree coral spectral reflectivity, and block Corallium Japonicum Kishinouye spectral reflectivity is with dendritic
There is certain diversity in Corallium Japonicum Kishinouye spectral reflectivity.Block Corallium Japonicum Kishinouye exists with tree coral spectral reflectivity first derivative
In 537nm-700nm wave band, waveform change is consistent, it is difficult to make a distinction block Corallium Japonicum Kishinouye with tree coral spectral reflectivity,
But in 430nm-479nm wave band, block Corallium Japonicum Kishinouye, can be to bulk higher than tree coral spectral reflectivity in the range of short-wave band
Corallium Japonicum Kishinouye makes a distinction with tree coral.Block Corallium Japonicum Kishinouye and tree coral second dervative waveform in visible light wave range changes difference not
Substantially, but block Corallium Japonicum Kishinouye can be made a distinction with tree coral at 483nm, 498nm and 514nm.Therefore, spectrum
Block Corallium Japonicum Kishinouye can be made a distinction by reflectance to a certain extent with tree coral, this conclusion and Hedley et al. thought block
The conclusion that shape Corallium Japonicum Kishinouye can make a distinction with tree coral spectral reflectivity is consistent (Hedley et al, 2004).And and Holden
Et al. the form of thought Corallium Japonicum Kishinouye on the Corallium Japonicum Kishinouye spectral reflectivity little conclusion inconsistent (Holden et al, 1999) of impact.With
Time, by block and tree coral spectral reflectivity first derivative and block Corallium Japonicum Kishinouye and tree coral derivative analysis of second, it is known that
Both diversityes can preferably be made a distinction by spectral reflectivity first derivative.
Contrast experiment:
This contrast experiment is to carry spectrogrph that fiber lengths is 25 meters in field measurement, specially changes up ship and arrives and to measure
Marine site, project cost consulting item sample place, wherein a people aboard ship operates spectrogrph, a people or two people diving and finds target Corallium Japonicum Kishinouye sample
Product, adjustment of being popped one's head in by spectrogrph is perpendicular to field project cost consulting item sample surfaces, measure spectrum reflectance.The measurement time is necessary
Being limited between the 12::00-14:00 at fine day noon, during measurement, diver reduces the disturbance to water body as far as possible, as far as possible without
Utensil influential on light field around.Owing to needs are dived under water, measure expansion and get up the bad situation of relative difficulty, such as sea situation
Under can not measure, the weather condition such as cloudy, cloudy can not be measured, and not only wastes time and energy, and some place can't complete
Measure.
The present invention departs from natural conditions restriction so that spectral reflectivity is measured, and the most remote marine site, as long as can carry
Counter sample product can complete laboratory measurement.Through repeatedly contrasting same breed project cost consulting item sample spectra Reflectivity spectral line
Basically identical, on the premise of ensureing to measure quality, drastically increase efficiency.
Claims (7)
1. a bank base project cost consulting item spectral measurement method, it is characterised in that comprise the following steps:
There is the cylinder body of black non transparent, be provided with water inlet in the lower end of cylinder body, upper end is provided with outlet, places black in cylinder body
The iron stand of color, sea water enters from water inlet, and outlet overflows, and keeps sea water flowing, is then put by project cost consulting item sample blocks
Enter to fill on the iron stand in the cylinder body of sea water, then use light source to irradiate project cost consulting item sample blocks, and use spectrometer measurement
The spectral reflectivity of project cost consulting item sample blocks, by spectrogrph probe vertical in project cost consulting item sample blocks surface during measurement, repeatedly
Measurement is averaged.
Bank base project cost consulting item spectral measurement method the most according to claim 1, it is characterised in that the illumination of described light source
Intensity is 106μmol/m2/s。
Bank base project cost consulting item spectral measurement method the most according to claim 2, it is characterised in that described light source includes wavelength
It it is the visible ray of 400~700nm.
Bank base project cost consulting item spectral measurement method the most according to claim 1, it is characterised in that described holding sea water stream
Dynamic, its ocean temperature is 26 DEG C~28 DEG C, and its seawater velocity is 0.5L/min.
Bank base project cost consulting item spectral measurement method the most according to claim 1, it is characterised in that described black non transparent
Cylinder body be inwall paste black wallpaper, diapire lay cover black nylon cloth glass jar, the brandreth of described black
Platform is the iron stand of black electrical adhesive tape looping.
Bank base project cost consulting item spectral measurement method the most according to claim 1, it is characterised in that described employing spectrogrph
Measuring the spectral reflectivity of project cost consulting item sample blocks, wavelength interval when it is measured is 0.5~1nm, and smooth 3 times.
Bank base project cost consulting item spectral measurement method the most according to claim 1, it is characterised in that described employing spectrogrph
Measure the spectral reflectivity of project cost consulting item sample blocks, by spectrogrph probe vertical in project cost consulting item sample blocks table during measurement
Face, when being 25 ° when using the spectrogrph probe angle of visual field, the diameter >=50mm of project cost consulting item sample blocks, or be when tested
During the full visual field, the diameter >=100mm of project cost consulting item sample blocks, described spectrogrph probe and project cost consulting item sample blocks
Distance is 5cm.
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Cited By (5)
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CN108088819A (en) * | 2018-02-07 | 2018-05-29 | 中国科学院南海海洋研究所 | A kind of hand-held sediment underwater spectral measurement instrument |
CN108267427A (en) * | 2018-02-07 | 2018-07-10 | 中国科学院南海海洋研究所 | Sediment spectral measurement method and equipment and terminal |
CN108333122A (en) * | 2018-02-07 | 2018-07-27 | 中国科学院南海海洋研究所 | Spectral measurement system and measurement method under sediment interfacial water |
WO2019196154A1 (en) * | 2018-04-12 | 2019-10-17 | 中国科学院南海海洋研究所 | Apparatus and method for measuring reflectivity of seabed sediments |
CN110411962A (en) * | 2019-08-03 | 2019-11-05 | 国家海洋环境监测中心 | Coral reef bleaching remote sensing monitoring mechanism based method analysis |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108088819A (en) * | 2018-02-07 | 2018-05-29 | 中国科学院南海海洋研究所 | A kind of hand-held sediment underwater spectral measurement instrument |
CN108267427A (en) * | 2018-02-07 | 2018-07-10 | 中国科学院南海海洋研究所 | Sediment spectral measurement method and equipment and terminal |
CN108333122A (en) * | 2018-02-07 | 2018-07-27 | 中国科学院南海海洋研究所 | Spectral measurement system and measurement method under sediment interfacial water |
CN108333122B (en) * | 2018-02-07 | 2019-12-31 | 中国科学院南海海洋研究所 | Submarine substrate interface underwater spectrum measurement system and measurement method |
CN108088819B (en) * | 2018-02-07 | 2020-08-18 | 中国科学院南海海洋研究所 | Hand-held type seabed bottom matter is spectral measurement appearance under water |
WO2019196154A1 (en) * | 2018-04-12 | 2019-10-17 | 中国科学院南海海洋研究所 | Apparatus and method for measuring reflectivity of seabed sediments |
US11162891B2 (en) * | 2018-04-12 | 2021-11-02 | South China Sea Institute Of Oceanology, Chinese Academy Of Sciences | Apparatus and method for measuring reflectivity of seabed sediments |
CN110411962A (en) * | 2019-08-03 | 2019-11-05 | 国家海洋环境监测中心 | Coral reef bleaching remote sensing monitoring mechanism based method analysis |
CN110411962B (en) * | 2019-08-03 | 2022-04-08 | 国家海洋环境监测中心 | Coral reef whitening remote sensing monitoring mechanism analysis method |
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