CN1040030A - The extraction process of effective component of paeonia lactiflora - Google Patents
The extraction process of effective component of paeonia lactiflora Download PDFInfo
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- CN1040030A CN1040030A CN 88104779 CN88104779A CN1040030A CN 1040030 A CN1040030 A CN 1040030A CN 88104779 CN88104779 CN 88104779 CN 88104779 A CN88104779 A CN 88104779A CN 1040030 A CN1040030 A CN 1040030A
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- CN
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- Prior art keywords
- nahco
- technology
- effective component
- root
- paeonia lactiflora
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Abstract
This technology is a kind of extracting method that improves effective component of paeonia lactiflora, it is characterized in that with sodium bicarbonate (NaHCO
3) solution replaces the ether in original technology, dilute neutralization, and then extract with ethyl acetate.This technology of having improved has equipment, operation is simple, and with short production cycle, raw material sources are wide, solvent safety coefficient height.With the product of this explained hereafter,, confirm the toxicological harmless effect, the characteristics that effect is good through pharmacological testing and clinical use.
Description
The invention relates to a kind of technology of from Chinese medicinal materials, extracting Chinese medicinal ingredients, specifically relate to from the root of herbaceous peony, extract the production technique of effective ingredient.
The root of herbaceous peony is a kind of Chinese medicine of having many uses, and it is enriched blood except having, astringe yin, gentle hepatic precordial pain, and clear and coherent blood vessels except that blood-arthralgia, receive the effect such as stomach Qi, and also have functions such as spasmolysis, analgesia, anti-frightened, anti-inflammatory, adjustment blood flow and invigorating blood circulation.Research in recent years also shows, the root of herbaceous peony is to the intensive carcinogenic substance, flavacin B
1Stronger detoxification is arranged.When more and more being paid attention to by people along with traditional herbal cuisine and dietetic food, the protective foods that adds Radix Paeoniae Alba extract also just arises at the historic moment, and these all provide extremely broad application prospect for the development and use of the root of herbaceous peony.Effective ingredient in the root of herbaceous peony is mainly glucoside, thereby, can more effectively from the root of herbaceous peony, receive the technology that this compound extracts, then be very important for the comprehensive development and utilization of the root of herbaceous peony.Domestic some extracting method of having reported all use ether as solvent, and its shortcoming is that boiling point, the burning-point of ether is low, and is therefore, inflammable, explosive, the safety precaution difficulty, in addition, ether toxicity is big, price is higher, and materials consumption is serious aborning, and this is to reducing cost, and it is disadvantageous increasing profit.
The objective of the invention is to propose the improvement technology that a kind of comparatively economical and practical and safe effective composition from white peony root extracts, it is characterized in that using NaHCO
3Solution replaces ether, dilutes neutralization, and then extracts with ethyl acetate, and it has equipment, operation is simple, and with short production cycle, raw material sources are wide, solvent safety coefficient height.With the product of this explained hereafter,, confirm the toxicological harmless effect, the characteristics that effect is good through pharmacological testing and clinical use.
The used chemical reagent of the present invention is commercially available analysis pure grade, and the root of herbaceous peony is grow radix paeoniae alba, the new root of herbaceous peony or oldly deposits the root of herbaceous peony.
The improvement technology that the present invention proposes mainly comprises pulverizing, extracts, concentrates, dilutes, extracts, and steps such as reconcentration and vacuum-drying, its concrete technology is as follows:
1) the RADIX PAEONIAE ALBA root is got in pulverizing, advances pulverizer and pulverizes, and crosses 60 mesh sieves and gets the root powder.
2) extract the root powder with 95% alcohol immersion 30 minutes, 85 ℃ of reflux of water-bath 3 hours, filtering separation extracting solution and filter residue.Filter residue remakes same processing three times.Last united extraction liquid.
3) concentrate water-bath and heat distillating extracting liquid for about 90 ℃,, promptly stop, getting acid concentrated solution when distillation temperature is raised to 85 ℃ to reclaim ethanol.
4) dilute with 0.05～0.2M NaHCO
3Aqueous solution dilution, and the acid concentrated solution that neutralizes, to solution PH be 4～6, room temperature left standstill 10～15 minutes, discharging the carbonic acid gas that produces, the slightly acidic soup.
5) extraction merges the 1st, 2,3,4 extraction fat layer and carries out reconcentration with ethyl acetate extraction slightly acidic soup.
6) reconcentration is in 80～90 ℃ of water-baths, and distillation ethyl acetate phase to reclaim ethyl acetate, gets pale brown look fluid extract.
7) vacuum-drying is put into vacuum drying oven with fluid extract, at 20mmHg, carries out vacuum-drying under 90～100 ℃, promptly gets faint yellow finished product.Content of paeoniflorin is greater than 60% in the finished product, and harmful element such as As, Pb, Hg, CO concentration respectively are no more than 3PPm after measured.
Below be to further specify the present invention by embodiment.
Embodiment 1
Take by weighing root powder 500 grams and place round-bottomed flask, add 1000 milliliter of 95% ethanol, reflux is 3 hours in 85～90 ℃ of water-baths.Use filter paper filtering, with separation and Extraction liquid and filter residue.Adding 700 milliliter of 95% ethanol in the filter residue handles three times equally.United extraction liquid places distilling flask, and 90 ℃ of water-bath distillation extracting solutions are to reclaim ethanol.When distillation temperature rises to 85 ℃, stop distillation, obtain PH and be about 3.0 concentrated solution.Add 0.1M NaHCO with concentrated solution equivalent
3Aqueous solution dilution (the control solution PH is 5), get the slightly acidic soup, soup is changed in the separating funnel, is 1: 3 in the ratio of soup and ethyl acetate, adds ethyl acetate extraction 9 times, the ethyl acetate layer that merges the 1st, 2,3,4 time is in distilling flask, ethyl acetate is reclaimed in 80～90 ℃ of distillations of water-bath, just is condensed into fluid extract, puts into vacuum drying oven, carry out vacuum-drying 20mmHg, 90 ℃, 15 gram faint yellow powdery finished products (yield is about 3%).Through check, content of paeoniflorin has mould element such as As, Pb, Hg, Co concentration after measured greater than 60%, respectively is no more than 3PPm.
Embodiment 2
Experimental procedure is with embodiment 1, but NaHCO
3Use other concentration instead, and the comparative product quality.
| NaHCO 3 | 1.0M | 0.5M | 0.2M | 0.1M | 0.05M | 0.01M |
| Product | Difficult setting | Yield is low | The yield high quality is good | The yield high quality is good | The yield high quality is good | Of poor quality |
Embodiment 3
Experimental procedure is with embodiment 1, NaHCO
3Change other weak base of 0.1M into, and the comparative product quality.
| The weak base kind | Na 2SO 3 | Na 2CO 3 | Na 2HPO 3 | NaHCO 3 |
| Product | Be difficult for dry, of poor quality | Of poor quality | Yield is higher, and is of poor quality | The yield height, the quality height |
According to the result of embodiment 2,3, NaHCO in the production
3Concentration should be chosen as 0.1M.As select Na
2CO
3, its concentration will be than NaHCO
3Be paper, other weak base should not be selected for use.
Claims (2)
1, a kind of technology of extracting effective composition from white peony root mainly comprises pulverizing, extraction, concentrates, dilutes, extracts.Step such as reconcentration and vacuum-drying is characterized in that using NaHCO
3And Na
2CO
3Solution is as the dilution neutralizing agent.Wherein with NaHCO
3Solution is good.
2, technology according to claim 1 is characterized in that used NaHCO
3And Na
2CO
3The concentration of solution is 0.05～0.2M.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88104779 CN1020907C (en) | 1988-07-29 | 1988-07-29 | Extraction process for effective component of paeonia lactiflora |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88104779 CN1020907C (en) | 1988-07-29 | 1988-07-29 | Extraction process for effective component of paeonia lactiflora |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1040030A true CN1040030A (en) | 1990-02-28 |
| CN1020907C CN1020907C (en) | 1993-05-26 |
Family
ID=4833140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 88104779 Expired - Fee Related CN1020907C (en) | 1988-07-29 | 1988-07-29 | Extraction process for effective component of paeonia lactiflora |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1020907C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107507C (en) * | 1999-05-10 | 2003-05-07 | 中国医学科学院药用植物研究所 | Immunosuppressive medicine |
| WO2005074952A1 (en) * | 2004-02-05 | 2005-08-18 | Chengdu Kanghong Technology Enterprises (Group) Co., Ltd. | Chinese medicine for treatment of irritable bowel sysndrome and the preparation thereof |
| CN102188503A (en) * | 2010-03-19 | 2011-09-21 | 傅宏征 | Preparation method of total glucosides from Paeonia lactiflora Pall., and Paeonia lactiflora Pall. total glucoside preparation and use thereof |
| CN103285101A (en) * | 2013-05-14 | 2013-09-11 | 亳州千草药业有限公司 | Method for extracting over-ground part of white peony root |
| CN113820422A (en) * | 2021-09-24 | 2021-12-21 | 宁波立华制药有限公司 | Method for detecting total glucosides of paeony by fingerprint spectrum |
-
1988
- 1988-07-29 CN CN 88104779 patent/CN1020907C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107507C (en) * | 1999-05-10 | 2003-05-07 | 中国医学科学院药用植物研究所 | Immunosuppressive medicine |
| WO2005074952A1 (en) * | 2004-02-05 | 2005-08-18 | Chengdu Kanghong Technology Enterprises (Group) Co., Ltd. | Chinese medicine for treatment of irritable bowel sysndrome and the preparation thereof |
| CN102188503A (en) * | 2010-03-19 | 2011-09-21 | 傅宏征 | Preparation method of total glucosides from Paeonia lactiflora Pall., and Paeonia lactiflora Pall. total glucoside preparation and use thereof |
| CN103285101A (en) * | 2013-05-14 | 2013-09-11 | 亳州千草药业有限公司 | Method for extracting over-ground part of white peony root |
| CN113820422A (en) * | 2021-09-24 | 2021-12-21 | 宁波立华制药有限公司 | Method for detecting total glucosides of paeony by fingerprint spectrum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1020907C (en) | 1993-05-26 |
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