CN103960233A - Controlled-release bacteriostatic agent and preparation method thereof - Google Patents
Controlled-release bacteriostatic agent and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a controlled-release bacteriostatic agent and a preparation method thereof. The controlled-release bacteriostatic agent is a pasty colloid obtained by mixing a controlled-release agent, a bactericide, an enhancer and a dissolving agent, and comprises the following effective components in parts by weight: 1,000 parts of sodium bentonite, 1.2-1.8 parts of hymexazol, 3.6-6.0 parts of resin acid copper salt, 24-36 parts of aluminum sulfate, and the balance of purified water. All the components are interacted to be prepared into grey white pasty colloid, so that the bacteria prevention and corrosion prevention effects are prominent, the characteristics and performance of various components are fully utilized, and the matching and dosage are scientific and reasonable; the bacteriostatic agent is closely combined with the production reality, is simple, convenient and feasible in application method, low in material price, easy to purchase materials, wide in application range, strong in practicability, and higher in popularization and application value.
Description
Technical field
The present invention relates to a kind of bacteriostatic agent, be applicable to the plant cuttages such as nursery stock, flowers, vegetables and grow seedlings, specifically, relate to a kind of slowly-releasing bacteriostatic agent and preparation method thereof, belong to plant asexual multiplication technology field.
Background technology
Cottage propagation has that reproduction speed is fast, inheritance changes the features such as little, is one of the most frequently used method of plant propagation, but in cottage propagation process, owing to being subject to infecting of various pathogens in cutting medium, very easily causes cuttings to be rotted; Particularly cuttage in summer, is subject to hot and humid impact, and in matrix, soon, infecting potential is strong, greatly affects cuttage survival rate in various pathogen breedings.How to carry out matrix sterilizing, form aseptic or few bacterium matrix, protection cuttings is not infected rotten, is a great problem that puzzlement plant cuttage is grown seedlings.
At present, the way that mainly uses bactericidal agent or high temperature treatment, to the matrix sterilizing that carries out disinfection, to reduce cuttage rotting rate, although can produce certain effect, exists significantly not enough.
Use bactericide to have the shortcomings such as short, the difficult grasp of dosage of lasting period, sterilizing be not thorough to the matrix sterilization impact of factor such as watered, and dosage is large, cost is high; The easy superinfection germ of high-temperature sterilization and be not suitable for large-scale production.
Summary of the invention
The technical problem to be solved in the present invention is for above deficiency, provides the sterilizing of a kind of energy the antibacterial slowly-releasing bacteriostatic agent that prevents that cuttings from rotting again and the lasting period is long.
Meanwhile, the present invention also provides the preparation method of described bacteriostatic agent, has advantages of simple to operate, easy to use.
For solving above technical problem, the present invention by the following technical solutions: a kind of slowly-releasing bacteriostatic agent, is characterized in that: described slowly-releasing bacteriostatic agent is the pasty state colloid being mixed to get by sustained release agent, bactericide, hardening agent and lytic agent.
A kind of prioritization scheme, described sustained release agent is one or more in sodium bentonite, talcum powder, cyclodextrin;
Described bactericide is one or more in vicious spirit, copper rosinate, potassium permanganate, lanthanum sulfate, evil frost bacterium ester;
Described hardening agent is one or more in aluminum sulfate, ferrous sulfate, copper sulphate;
Described lytic agent is pure water.
Another kind of prioritization scheme, is characterized in that: effective composition and the parts by weight of described slowly-releasing bacteriostatic agent are: 1000 parts of sodium bentonites, dislike 24～36 parts, mould clever 1.2～1.8 parts, 3.6～6.0 parts of copper rosinates and aluminum sulfate, surplus is pure water.
Another prioritization scheme, effective composition and the parts by weight of described bacteriostatic agent are: 1000 parts of sodium bentonites, dislike 3600 parts of mould clever 1.2 parts, 3.6 parts of copper rosinates, 24 parts, aluminum sulfate and pure water.
Adopt this technical scheme, be particularly useful for germ reproduction weak cuttage period, described slowly-releasing bacteriostatic agent is for cuttage Chinese rose in spring, and the rotting rate of Chinese rose cuttings is 0.
Further prioritization scheme, effective composition and the parts by weight of described slowly-releasing bacteriostatic agent are: 1000 parts of sodium bentonites, dislike 3600 parts of mould clever 1.8 parts, 6.0 parts of copper rosinates, 36 parts, aluminum sulfate and pure water.
Adopt this technical scheme, be particularly useful for the cuttage period that germ reproduction is stronger, described bacteriostatic agent is for epicormic branch cuttage lilac in summer, and the rotting rate of lilac cuttings is 1%.
In above raw material:
Sodium bentonite is as the sustained release agent in the present invention, when sodium bentonite contacts with water, and can be with aluminum sulfate, dislike the cotton-shaped hydrate that mould spirit, copper rosinate form and fully merge, form pasty state colloid, stable in properties, has reduced the active ingredient causing because watering and has run off, and its slow releasing function is remarkable; Utilize the moisture expantion characteristic of sodium bentonite, can make cuttings water content around keep relative stability, reduce water spray number of times; Utilize pulping and the viscosity of sodium bentonite; can make described bacteriostatic agent adhere to, be wrapped in cuttings surface and form holding effect, stable protective layer; can isolate cuttings contacts with matrix; around cuttings, form aseptic area; the path of infecting that makes bactericide wherein can kill pathogen in matrix effectively to block again pathogen, reaches the effect that reduces cuttings rotting rate.
Sodium bentonite is the nonmetallic minerals taking montmorillonite as essential mineral composition, there is some cation in the layer structure forming at montmorillonite structure cell, in the time that sodium bentonite contacts with water, these interlayer cations have one to dissociate in water, the trend of diffusion, along with cationic disengaging, bentonite surface band negative electricity, now can produce Electrostatic Absorption to the cation in solution, wherein a part of cation is closely inhaled mutually with sodium bentonite, form adsorption layer, remaining cation is evenly distributed in liquid phase around and forms diffusion layer with their aqueous solvent, by this electric double layer characterization of adsorption of sodium bentonite, bactericide is adsorbed and is gathered into condensation product, there is very strong stability and lasting slow release.
Dislike mould spirit and copper rosinate as bactericide, dislike the bactericide that mould spirit is the environmental protection of a kind of broad-spectrum high-efficiency low-toxicity, soil disinfectant, again a plant growth regulators simultaneously, disliking mould spirit has efficiently the pythium spp in soil, in soil, can be combined with inorganic aluminium ion, improve the sprouting ability that suppresses germ spore, very competent by adsorption by soil, mobility in vertical and horizontal direction is very little, efficacy stability and lasting period reach more than 2 weeks, dislike mould spirit and there is interior absorption and conductivity, its metabolite in plant corpus is two kinds of glucosides, thereby can improve physiologically active of plant and can promote plant strain growth, tillering of root, the increase of root hair and the activity of root improve, disliking mould spirit does not exert an influence to the ecology of Soil Microorganism, in soil, can resolve into the compound that toxicity is very low, safe noresidue, copper rosinate is a kind of disinfecting copper agent of high-efficiency low-toxicity wide spectrum, and the lasting period is long, has prevention protection and treatment double action, can not only prevent and treat multiple fungi, still prevents and treats the conventional medicament of bacteriosis, also has the effect that stimulates plant growth simultaneously.Dislike mould spirit and copper rosinate and use simultaneously, mutually make up, can effectively prevent and treat the cuttings that various fungus and bacteriums cause and rot.
Aluminum sulfate (Al
2(SO4)
3) as hardening agent, aluminum sulfate has strengthening sterilization and strengthening slowly-releasing double action, after aluminum sulfate is water-soluble, the aluminium ion of its positive charge and disliking between the electronegative hydroxyl of mould spirit attracts each other, greatly improve and dislike the antibacterial corrosion-resistant ability of mould spirit, drug effect reaches more than 2 weeks, and strengthening bactericidal effect is obvious; Aluminum sulfate aqueous solution can form more stable cotton-shaped hydrate by bactericide absorption, mutually merges with sodium bentonite suspension, has strengthened slow release.In addition, aluminum sulfate is also a kind of effectively mollusk insecticide, and the software insect in matrix is had to prevention and control capability.
Pure water is lytic agent.
Sodium bentonite, aluminum sulfate, the mould spirit of evil, copper rosinate organically combine under the effect of lytic agent pure water, interact, and form one, and sterilizing is antibacterial prevents that cuttings from rotting and the lasting period is long.
Based on above bacteriostatic agent, the present invention also provides a kind of preparation method of described bacteriostatic agent, it is characterized in that: described preparation method comprises the following steps:
1) get each raw material according to weight part ratio;
2) mould evil spirit, copper rosinate, aluminum sulfate are added successively in pure water and stirred;
4) add again sodium bentonite and stir, making bentonite and liquid fully be fused into pasty state colloid.
A kind of concrete prioritization scheme, the mixing time in described step 4) is 10min.
The present invention adopts after above technical scheme, compared with prior art, has the following advantages:
1, the swelling of sustained release agent sodium base, by the fixing each component material absorption pasty state colloid that forms, forms medicament layer on cuttings surface, and the impact of the factor such as substantially do not watered, continues to play a role for a long time, greatly extends the each component lasting period, and its slow releasing function is remarkable;
2, the selection of sustained release agent, bactericide and hardening agent and proportioning take full advantage of its characteristic and performance, have strengthened the effect of sterilization and slowly-releasing, and effect significantly;
3, specific aim and practical, selection cheapness is easily purchased, applied widely, is beneficial to application and promotes.
In a word, the present invention adopts above technical scheme, and antibacterial antiseptic effect is outstanding, and takes full advantage of the characteristic and performance of each composition, and proportioning consumption is scientific and reasonable; Close with the actual combination of production, using method is simple and easy to do, and material cheapness is easily purchased, applied widely, has very strong practicality, has higher utilization and extention and is worth.
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment
Embodiment 1, a kind of slowly-releasing bacteriostatic agent, it effectively forms and parts by weight are: 1000 parts of sodium bentonites, dislike 3600 parts of mould clever 1.2 parts, 3.6 parts of copper rosinates, 24 parts, aluminum sulfate and pure water.
Described sodium bentonite is produced by Weifang Hao Da bentonite Co., Ltd;
The mould spirit of described evil adopts the mould spirit of evil that former medicine is 99%, and by Weifang, Hua Nuo bio tech ltd produces;
Described copper rosinate is the copper rosinate containing 20% missible oil, and by Bai He source, Qingdao, biotechnology Co., Ltd produces;
Described aluminum sulfate is that the analysis of being produced by Bo Di chemical inc, Tianjin is pure;
Described slowly-releasing bacteriostatic agent adopts following steps to be prepared:
1) get each raw material according to weight part ratio;
2) mould evil spirit, copper rosinate, aluminum sulfate are added successively in pure water and stirred;
3) add again sodium bentonite and stir 10min, making bentonite and liquid fully be fused into pasty state colloid.
Using method: the plant cuttings cutting is quickly dipped in the pasty state colloid preparing by cutting, and makes slowly-releasing bacteriostatic agent evenly be attached to cuttings surface, forms medicament layer.
Comparative trial: taking spring cuttage Chinese rose as subjects, choosing 300 strain Chinese rose cuttings tests, described 300 strain Chinese rose cuttings are divided into 3 experimental group at random, each experimental group 100 strains, experimental group is used the slowly-releasing bacteriostatic agent of gained in the present embodiment, Chinese rose cuttings is quickly dipped in slowly-releasing bacteriostatic agent, then in matrix, punch, Chinese rose cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 1 uses the agent of cuttage conventional sterilization, that is: the mould clever solution of the evil of 500mg/L, Chinese rose cuttings is quickly dipped in the mould clever solution of evil, then in matrix, punch, Chinese rose cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 2 is left intact, cuttings cuttage.
Deliberated index: selecting rooting rate, rotting rate and callus rate is deliberated index, wherein: rooting rate refers to that the cuttings of taking root accounts for the percentage of total cuttings, rotting rate refers to that rotten cuttings accounts for the percentage of total cuttings, and callus rate refers to and forms callus but the also unputrefied cuttings of not taking root accounts for the percentage of total cuttings.
Taking root of Chinese rose cuttings and rotten situation are checked in cuttage after 45 days, the result obtaining is as following table:
| Contrast group | Rooting rate/% | Rotting rate/% | Callus rate/% |
| Experimental group | 71 | 0 | 29 |
| Control group 1 | 59 | 25 | 16 |
| Control group 2 | 46 | 50 | 4 |
From the above results, use the slowly-releasing bacteriostatic agent of the present embodiment preparation, spring, the rotting rate of cuttage Chinese rose reduced by 25%～50%, and rooting rate improves 12%～25%, and antibacterial Perishing prevention effect is remarkable, also has certain short rooting efficiency simultaneously.。
Embodiment 2, a kind of slowly-releasing bacteriostatic agent, it effectively forms and parts by weight are: 1000 parts of sodium bentonites, dislike 3600 parts of mould clever 1.8 parts, 6.0 parts of copper rosinates, 36 parts, aluminum sulfate and pure water.
Described sodium bentonite is produced by Weifang Hao Da bentonite Co., Ltd;
The mould spirit of described evil adopts the mould spirit of evil that former medicine is 99%, and by Weifang, Hua Nuo bio tech ltd produces;
Described copper rosinate is the copper rosinate containing 20% missible oil, and by Bai He source, Qingdao, biotechnology Co., Ltd produces;
Described aluminum sulfate is that the analysis of being produced by Bo Di chemical inc, Tianjin is pure.
Described slowly-releasing bacteriostatic agent adopts following steps to be prepared:
1) get each raw material according to weight part ratio;
2) mould evil spirit, copper rosinate, aluminum sulfate are added successively in pure water and stirred;
4) add again sodium bentonite and stir 10min, making bentonite and liquid fully be fused into pasty state colloid.
Using method: the plant cuttings cutting is quickly dipped in the pasty state colloid preparing by cutting, and makes slowly-releasing bacteriostatic agent evenly be attached to cuttings surface, forms medicament layer.
Comparative trial: taking summer epicormic branch cuttage lilac as subjects, choosing 300 strain lilac cuttings tests, described 300 strain lilac cuttings are divided into 3 experimental group at random, each experimental group 100 strains, experimental group is used the slowly-releasing bacteriostatic agent of gained in the present embodiment, lilac cuttings is quickly dipped in slowly-releasing bacteriostatic agent, then in matrix, punch, lilac cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 1 uses conventional sterilization agent, that is: the mould clever solution of the evil of 500mg/L, lilac cuttings is quickly dipped in vicious clever solution, then in matrix, punch, lilac cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 2 is left intact, cuttings cuttage.
Deliberated index: selecting rooting rate, rotting rate and callus rate is deliberated index, wherein: rooting rate refers to that the cuttings of taking root accounts for the percentage of total cuttings, rotting rate refers to that rotten cuttings accounts for the percentage of total cuttings, and callus rate refers to and forms callus but the also unputrefied cuttings of not taking root accounts for the percentage of total cuttings.
Taking root of lilac cuttings and rotten situation are checked in cuttage after 45 days, the result obtaining is as following table:
| Contrast group | Rooting rate/% | Rotting rate/% | Callus rate/% |
| Experimental group | 65 | 1 | 34 |
| Control group 1 | 51 | 35 | 14 |
| Control group 2 | 38 | 56 | 6 |
From the above results, use the slowly-releasing bacteriostatic agent of the present embodiment preparation, summer, the rotting rate of epicormic branch cuttage lilac reduced by 34%～55%, and rooting rate improves 14%～27%, and antibacterial Perishing prevention effect is remarkable, also has certain short rooting efficiency simultaneously.
Embodiment 3, a kind of slowly-releasing bacteriostatic agent, it effectively forms and parts by weight are: 1000 parts of talcum powder, dislike mould clever 1.2 parts, 3.6 parts, potassium permanganate, dislike 1.8 parts of white bacterium esters, 3600 parts of 24 parts, ferrous sulfate and pure water.
Above-mentioned raw materials is mixed into pasty state colloid by weight.
Using method: the plant cuttings cutting is quickly dipped in the pasty state colloid preparing by cutting, and makes slowly-releasing bacteriostatic agent evenly be attached to cuttings surface, forms medicament layer.
Comparative trial: taking spring cuttage Chinese rose as subjects, choosing 300 strain Chinese rose cuttings tests, described 300 strain Chinese rose cuttings are divided into 3 experimental group at random, each experimental group 100 strains, experimental group is used the slowly-releasing bacteriostatic agent of gained in the present embodiment, Chinese rose cuttings is quickly dipped in slowly-releasing bacteriostatic agent, then in matrix, punch, Chinese rose cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 1 uses cuttage common fungicide, that is: the mould clever solution of the evil of 500mg/L, Chinese rose cuttings is quickly dipped in vicious clever solution, then in matrix, punch, Chinese rose cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 2 is left intact, cuttings cuttage.
Deliberated index: selecting rooting rate, rotting rate and callus rate is deliberated index, wherein: rooting rate refers to that the cuttings of taking root accounts for the percentage of total cuttings, rotting rate refers to that rotten cuttings accounts for the percentage of total cuttings, and callus rate refers to and forms callus but the also unputrefied cuttings of not taking root accounts for the percentage of total cuttings.
Taking root of Chinese rose cuttings and rotten situation are checked in cuttage after 45 days, the result obtaining is as following table:
| Contrast group | Rooting rate/% | Rotting rate/% | Callus rate/% |
| Experimental group | 67 | 5 | 28 |
| Control group 1 | 65 | 25 | 10 |
| Control group 2 | 46 | 50 | 4 |
From the above results, use the slowly-releasing bacteriostatic agent of the present embodiment preparation, spring, the rooting rate of cuttage Chinese rose improved 2%～21%, and rotting rate reduces by 20%～45%, and antibacterial Perishing prevention effect is remarkable, and short rooting efficiency compares indifference with vicious clever solution phase.
Embodiment 4,
A kind of slowly-releasing bacteriostatic agent, it effectively forms and parts by weight are: 1000 parts of cyclodextrin, 1.8 parts of lanthanum sulfates, dislike 3600 parts of 3.6 parts of white bacterium esters, 6.0 parts of copper rosinates, 36 parts, copper sulphate and pure water.
Above-mentioned raw materials is mixed into pasty state colloid by weight.
Using method: the plant cuttings cutting is quickly dipped in the pasty state colloid preparing by cutting, and makes slowly-releasing bacteriostatic agent evenly be attached to cuttings surface, forms medicament layer.
Comparative trial: taking summer epicormic branch cuttage lilac as subjects, choosing 300 strain lilac cuttings tests, described 300 strain lilac cuttings are divided into 3 experimental group at random, each experimental group 100 strains, experimental group is used the slowly-releasing bacteriostatic agent of gained in the present embodiment, lilac cuttings is quickly dipped in slowly-releasing bacteriostatic agent, then in matrix, punch, lilac cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 1 uses cuttage common fungicide, that is: the mould clever solution of the evil of 500mg/L, lilac cuttings is quickly dipped in vicious clever solution, then in matrix, punch, lilac cuttings after quickly dipped is put into hole by reality, enter Routine Management, control group 2 is left intact, cuttings cuttage.
Deliberated index: selecting rooting rate, rotting rate and callus rate is deliberated index, wherein: rooting rate refers to that the cuttings of taking root accounts for the percentage of total cuttings, rotting rate refers to that rotten cuttings accounts for the percentage of total cuttings, and callus rate refers to and forms callus but the also unputrefied cuttings of not taking root accounts for the percentage of total cuttings.
Taking root of lilac cuttings and rotten situation are checked in cuttage after 45 days, the result obtaining is as following table:
| Contrast group | Rooting rate/% | Rotting rate/% | Callus rate/% |
| Experimental group | 60 | 18 | 22 |
| Control group 1 | 57 | 35 | 8 |
| Control group 2 | 38 | 56 | 6 |
From the above results, use the slowly-releasing bacteriostatic agent of the present embodiment preparation, summer, the rooting rate of epicormic branch cuttage lilac improved 3%～22%, and rotting rate reduces by 17%～38%, and antibacterial Perishing prevention effect is remarkable, and short rooting efficiency compares indifference with vicious clever solution phase.
Sustained release agent can adopt sodium bentonite, talcum powder, one or more in cyclodextrin, bactericide can adopt vicious spirit, copper rosinate, potassium permanganate, lanthanum sulfate, dislike one or more in white bacterium ester, hardening agent is aluminum sulfate, ferrous sulfate, one or more in copper sulphate, lytic agent is pure water, through verification experimental verification, sustained release agent is selected sodium bentonite, bactericide adopts the mixture of vicious spirit and copper rosinate, hardening agent adopts aluminum sulfate, when lytic agent adopts pure water, the effect of slowly-releasing bacteriostatic agent is best, growth-promoting root and Perishing prevention effect highly significant.
The above is giving an example of best mode for carrying out the invention, and the part of wherein not addressing is in detail those of ordinary skill in the art's common practise.Protection scope of the present invention is as the criterion with the content of claim, and any equivalent transformation carrying out based on technology enlightenment of the present invention, also within protection scope of the present invention.
Claims (7)
1. a slowly-releasing bacteriostatic agent, is characterized in that: described slowly-releasing bacteriostatic agent is the pasty state colloid being mixed to get by sustained release agent, bactericide, hardening agent and lytic agent.
2. a kind of slowly-releasing bacteriostatic agent as claimed in claim 1, is characterized in that: described sustained release agent is one or more in sodium bentonite, talcum powder, cyclodextrin;
Described bactericide is one or more in vicious spirit, copper rosinate, potassium permanganate, lanthanum sulfate, evil frost bacterium ester;
Described hardening agent is one or more in aluminum sulfate, ferrous sulfate, copper sulphate;
Described lytic agent is pure water.
3. a kind of slowly-releasing bacteriostatic agent as claimed in claim 2, it is characterized in that: effective composition and the parts by weight of described slowly-releasing bacteriostatic agent are: 1000 parts of sodium bentonites, dislike 24～36 parts, mould clever 1.2～1.8 parts, 3.6～6.0 parts of copper rosinates and aluminum sulfate, surplus is pure water.
4. a kind of slowly-releasing bacteriostatic agent as claimed in claim 3, is characterized in that: effective composition and the parts by weight of described slowly-releasing bacteriostatic agent are: 1000 parts of sodium bentonites, dislike 3600 parts of mould clever 1.2 parts, 3.6 parts of copper rosinates, 24 parts, aluminum sulfate and pure water.
5. a kind of slowly-releasing bacteriostatic agent as claimed in claim 3, is characterized in that: effective composition and the parts by weight of described slowly-releasing bacteriostatic agent are: 1000 parts of sodium bentonites, dislike 3600 parts of mould clever 1.8 parts, 6.0 parts of copper rosinates, 36 parts, aluminum sulfate and pure water.
6. as the preparation method of the slowly-releasing bacteriostatic agent of claim 3-5 as described in one of them: it is characterized in that: as described in preparation method comprise the following steps:
1) get each raw material according to weight part ratio;
2) mould evil spirit, copper rosinate, aluminum sulfate are added successively in pure water and stirred;
3) add again sodium bentonite and stir, making bentonite and liquid fully be fused into pasty state colloid.
7. the preparation method of slowly-releasing bacteriostatic agent as claimed in claim 6, is characterized in that: the mixing time in described step 4) is 10min.
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| CN105532230A (en) * | 2015-12-21 | 2016-05-04 | 陈从云 | Sealed cuttage seedling-raising method with glue film |
| JP2017043559A (en) * | 2015-08-27 | 2017-03-02 | 住化エンバイロメンタルサイエンス株式会社 | Antibacterial agent composition |
| CN108157389A (en) * | 2018-03-16 | 2018-06-15 | 浙江绿龙生态科技有限公司 | Nursery stock transplanting is quickly survived root-growing agent and preparation method thereof |
| CN108441350A (en) * | 2018-03-23 | 2018-08-24 | 浙江工业大学 | A kind of kitchen cleaning agent and preparation method thereof based on bio-based compounds |
| CN108617653A (en) * | 2018-05-07 | 2018-10-09 | 柯江波 | Cutting propagation sustained release bacteriostatic agent and preparation method based on mudflat sludge |
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| CN108617653A (en) * | 2018-05-07 | 2018-10-09 | 柯江波 | Cutting propagation sustained release bacteriostatic agent and preparation method based on mudflat sludge |
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| CN103960233B (en) | 2015-09-23 |
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