A kind of composition of reducing blood lipid prepared with onion oil and preparation method thereof
Technical field
The present invention relates to onion oil, is composition of reducing blood lipid prepared by a kind of onion oil and preparation method thereof.
Background technology
With the extreme enrichment of material life, the crowd for suffering from hyperlipemia increases year by year, according to statistics, the youth of hyperlipemia
Change was also progressively developing in recent years.Because the Main Ingredients and Appearance in high fat of blood is triglycerides and cholesterol, when Hyperlipidemia, easily
Cause atherosclerotic, ultimately result in cardiovascular and cerebrovascular disease.Therefore, with the popularization of health education, people are to hyperlipemia
Attention degree improve a lot, reducing blood lipid of taking medicine is received by the patient of hyperlipemia.However, presently commercially available hypolipidemic master
If Western medicine and Chinese patent drug, substantially, but the internal organs such as the liver kidney to human body have an obvious side effect to Western medicine lipid-lowering effect, and in into
Medicine then needs long-term taking, the more difficult determination of curative effect.In addition disclosed antilipemic Chinese herbal medicine decoction, needs to do different according to different physique
Select, meanwhile, the preparation of decoction is complicated, effective slower.
The content of the invention
It is an object of the invention to provide a kind of composition of reducing blood lipid prepared with onion oil and preparation method thereof, it is adopted
Combined with the high-purity extract in natural plants, lipid-lowering effect substantially, has no toxic side effect, and is easy to carry and takes.
The present invention for achieving the above object, is achieved through the following technical solutions:A kind of reducing blood lipid prepared with onion oil
Composition, is made up of the raw material of following weight ratio:Onion oil 40-60, maize germ oil 25-35, resveratrol micro mist 13-25 and
Punica granatum L. polyphenol 0.85.
A kind of composition of described reducing blood lipid prepared with onion oil, is made up of the raw material of following weight ratio:Onion oil
54th, maize germ oil 30, resveratrol micro mist 15.15, Punica granatum L. polyphenol 0.85.
A kind of composition of described reducing blood lipid prepared with onion oil, is made up of the raw material of following weight ratio:Onion oil
60th, maize germ oil 25, resveratrol micro mist 14.15, Punica granatum L. polyphenol 0.85.
A kind of preparation method of the composition of described reducing blood lipid prepared with onion oil, comprises the steps:
(1) resveratrol micro mist 13-25 is taken by weight and Punica granatum L. polyphenol 0.85 is standby;
(2) onion oil is prepared:
(2.1) fresh onion is taken, crushing is centrifuged to obtain Conditions of Onion Juice;
(2.2) sodium carbonate, sodium carbonate addition is added to be the 1/8-1/2 of Conditions of Onion Juice volume, add after dissolving in Conditions of Onion Juice
Enter 95% ethanol or absolute ethyl alcohol, aqueous two-phase system is formed after being mixed, the addition of ethanol is identical with the volume of onion oil;
(2.3) phase in taking-up, reduced pressure concentration, rotary evaporation, obtain rough onion oil;
(2.4) remove phase, with chloroform and alcohol mixeding liquid extraction 30-40 minutes, after standing upper and lower phase, chloroform and
The volume ratio 5 of ethanol:1, mixed liquor is identical with lower phase volume;
(2.5) upper reduced pressure concentration, rotary evaporation, recycling design at 20-70 DEG C in step (2.4), obtains rough onion
Oil;
(2.6) the rough onion oil obtained in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and ethanol
Mixed solvent is eluted four times, and chloroform is followed successively by 9 with the volume ratio of ethanol:1、6:1、3:1、2.3:1, to gather the stream of onion oil
Part;
(2.7) by the stream part containing onion oil in 20-70 DEG C of decompression rotary evaporation, recycling design is dehydrated desalination, is refined
Onion oil, purity is 99.85%;
(3) maize germ oil is prepared:
(3.1) maize germ and corn peel are taken by 10-15:The ratio of 1-2 is well mixed after crushing, and obtains compound;
(3.2) add in compound by the 7-8% water intakings of compound gross weight, insert after stirring in extruder, to jade
Rice plumule and corn peel carry out after extrusion, obtaining expanded mixture;
(3.3) expanded mixture, water and wheat bran are taken it is well mixed and obtains mixed liquor, the weight of expanded mixture, water and wheat bran
Amount is than being 11-17:8-12:2-3, to sterilizing 10-20 minutes at 123 DEG C of mixed liquor, is cooled to 25-50 DEG C;
(3.4) Lactobacillus plantarum nutrient solution, the 0.67%-1.25% of 2%-2.5% volumes are added in the mixed liquor after cooling
The mycotic culture liquid of the bacillus nutrient solution of volume and 0.67%-1.25% balls product, at 30-50 DEG C, anaerobic condition fermentation 40-
80 hours, sterilize 10-20 minutes at 123 DEG C after fermentation;
(3.5) zymotic fluid after step (3.4) sterilizing is down to after room temperature and adds phosphoric acid, adjust pH to 4.8, centrifugation to separate
Obtain free oil, emulsion layer and residue;
(3.6) by the isolated free oil of emulsion layer;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains maize germ oil.
(4) onion oil, maize germ oil, resveratrol micro mist and Punica granatum L. polyphenol are mixed by weight, as reducing blood lipid
Composition.
A kind of preparation method of the composition of described reducing blood lipid prepared with onion oil, comprises the steps:
(1) resveratrol micro mist 15.15 is taken by weight and Punica granatum L. polyphenol 0.85 is standby;
(2) onion oil is prepared:
(2.1) the fresh onions of 1000g are taken, crushing is centrifuged to obtain 410ml Conditions of Onion Juice;
(2.2) 205g sodium carbonate is added in Conditions of Onion Juice, the ethanol or absolute ethyl alcohol of 410ml 95% is added after dissolving, mixed
Aqueous two-phase system is formed afterwards, and phase and phase on 400ml aqueous two-phase extractions under 420ml aqueous two-phase extractions are obtained after standing;
(2.3) phase in taking-up, reduced pressure concentration, rotary evaporation, obtain rough onion oil 95ml;
(2.4) phase is removed, with 420ml chloroforms and ethanol volume 5:1 mixed liquor is extracted 40 minutes, and 430ml is obtained after standing
Phase under upper phase and 400ml;
(2.5) upper reduced pressure concentration, rotary evaporation, recycling design at 20-70 DEG C in step (2.4), obtains rough onion
Oily 12ml;
(2.6) the rough onion oil obtained in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and ethanol
Mixed solvent is eluted four times, and chloroform is followed successively by 9 with the volume ratio of ethanol:1、6:1、3:1、2.3:1, to gather the stream of onion oil
Part;
(2.7) by the stream part containing onion oil in 20-70 DEG C of decompression rotary evaporation, recycling design is dehydrated desalination, is refined
Onion oil 49g, purity is 99.85%, yield 4.9%;
(3) maize germ oil is prepared:(3.1) take and be well mixed after 15kg maize germs and the crushing of 2kg corn peels, obtain
To compound;
(3.2) take 1.36kg water to add in compound, insert after stirring in extruder, to maize germ and maize seed
Skin is carried out after extrusion, obtains expanded mixture;
(3.3) take the expanded mixture in step (3.2) and be well mixed with 12kg water and 3kg wheat bran and obtain mixed liquor, it is swollen
Change mixture, to sterilizing 20 minutes at 123 DEG C of mixed liquor, be cooled to 50 DEG C;
(3.4) 500ml Lactobacillus plantarum nutrient solutions, 250ml bacillus nutrient solutions are added in the mixed liquor after cooling
And 250ml mycotic culture liquid, at 50 DEG C, anaerobic condition ferments 80 hours, sterilizes 20 minutes at 123 DEG C after fermentation;
(3.5) zymotic fluid after step (3.4) sterilizing is down to after room temperature and adds phosphoric acid, adjust pH to 4.8, centrifugation to separate
Obtain free oil, emulsion layer and residue;
(3.6) by the isolated free oil of emulsion layer;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains maize germ oil 7kg;
Maize germ oil yield is 99.9%, and purity is 99.94%;Content with high effective liquid chromatography for measuring free oil Linoleic acid is
55%;
(4) onion oil 54, maize germ oil 30, resveratrol micro mist 15.15 and Punica granatum L. polyphenol 0.85 are mixed by weight
, as blood-fat reducing composition.
The scallion oil that dissolves used in composition of the present invention is that a kind of special method is obtained, due to published extraction onion
The onion oil that the method for oil is obtained has bad smell, color deeper, and the effective ingredient loss of onion oil is larger during high temperature process,
The materials such as the onion oil that some solvent methods are produced, the pigment in solvent are easily remained, it is impossible to for human body long-term taking, main shadow
There is harmful effect to human body when ringing curative effect and long-term taking.It is pure with the onion oil of ad hoc approach of the present invention extraction without bad smell
Degree reaches 99. 85%, the loss without thermal sensitivity volatile component and destruction, and high income, and yield reaches 3.96-4.9%;Use
The purity of resveratrol micro mist is 99.5%;Punica granatum L. polyphenol is extracted as known method, it is desirable to the pomegranate of commercially available purity more than 85%
Polyphenol;Maize germ oil is extracted using ad hoc approach of the present invention, and with corn peel cracking agent is done, and is had to maize germ
Phasic property is good, does not produce pollution, and the characteristics of cell-wall breaking ratio is high, the purity of maize germ oil is 99.94%.The combination of the present invention
Onion oil in thing can reduce triglyceride and cholesterol in serum, and its amount for reducing triglyceride reaches nature slippage
More than 2 times, the amount for reducing cholesterol reaches more than 1.5 times of nature slippage, and research shows, onion oil is individually used for dropping blood
Fat, its effect has substantially reduction, but sulfur compound, flavonoids etc. more than 60 in onion oil than chemicalses such as Etofylline Clofibrate and clofibrates
Beneficial is planted, while with effect for reducing blood fat, is acted on stronger anti-oxidant and anti-platelet aggregation etc., further
Research shows, the content height of blood fat reflects the level of internal lipid metabolism, and work as artery and venous capillary elasticity compared with
When low, the level of relative of metabolism lipid is reduced, and when particularly lacking linoleic acid in vivo, cholesterol will be with saturated fatty acid group
Close, dysbolism occurs, precipitate on vascular wall, affect when platelet aggregation, antioxidant content are relatively low in blood
Lipids contents.The present invention is aided with maize germ oil, resveratrol micro mist and Punica granatum L. polyphenol, to improve blood vessel elasticity, reduces blood
Saturated fatty acid is combined with cholesterol in pipe, and contained antioxidant can capture and neutralize free radical, anti-platelet aggregation.It is dynamic
Thing experiment shows:The present composition has significantly synergy, the effect with obvious reducing blood lipid.The present composition is also
Endovascular harmful substance can be eliminated.The present composition is extracted in natural materials, is had no toxic side effect, and long-term taking is to people
Body has no adverse effects.The present composition makes capsule for reduction blood fat, services once sooner or later daily, each 3g, is within 10 days
One course for the treatment of.The material purity prepared with the method for the present invention in composition is high, can guarantee that the effect of reduction blood fat, the raw material of extraction
High income, it is easy to industrialized production, production process is without any pollution.
Description of the drawings
Fig. 1 is the obtained onion essential oil Jing GC-MS detections of the present invention, identify more than 60 kind sulfur-containing compounds always from
Subflow figure.
Specific embodiment
A kind of composition of reducing blood lipid prepared with onion oil of the present invention, is made up of the raw material of following weight ratio:Onion
Oily 40-60, maize germ oil 25-35, resveratrol micro mist 13-25 and Punica granatum L. polyphenol 0.85.
A kind of preparation method of the composition of the reducing blood lipid prepared with onion oil, comprises the steps:
(1) resveratrol micro mist 13-25 is taken by weight and Punica granatum L. polyphenol 0.85 is standby;
(2) onion oil is prepared:
(2.1) fresh onion is taken, crushing is centrifuged to obtain Conditions of Onion Juice;
(2.2) sodium carbonate, sodium carbonate addition is added to be the 1/8-1/2 of Conditions of Onion Juice volume, add after dissolving in Conditions of Onion Juice
Enter 95% ethanol or absolute ethyl alcohol, aqueous two-phase system is formed after being mixed, the addition of ethanol is identical with the volume of onion oil;
(2.3) phase in taking-up, reduced pressure concentration, rotary evaporation, obtain rough onion oil;
(2.4) remove phase, with chloroform and alcohol mixeding liquid extraction 30-40 minutes, after standing upper and lower phase, chloroform and
The volume ratio 5 of ethanol:1, mixed liquor is identical with lower phase volume;
(2.5) upper reduced pressure concentration, rotary evaporation, recycling design at 20-70 DEG C in step (2.4), obtains rough onion
Oil;
(2.6) the rough onion oil obtained in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and ethanol
Mixed solvent is eluted four times, and chloroform is followed successively by 9 with the volume ratio of ethanol:1、6:1、3:1、2.3:1, to gather the stream of onion oil
Part;
(2.7) by the stream part containing onion oil in 20-70 DEG C of decompression rotary evaporation, recycling design is dehydrated desalination, is refined
Onion oil, purity is 99.85%;
(3) maize germ oil is prepared:
(3.1) maize germ and corn peel are taken by 10-15:The ratio of 1-2 is well mixed after crushing, and obtains compound;
(3.2) add in compound by the 7-8% water intakings of compound gross weight, insert after stirring in extruder, to jade
Rice plumule and corn peel carry out after extrusion, obtaining expanded mixture;
(3.3) expanded mixture, water and wheat bran are taken it is well mixed and obtains mixed liquor, the weight of expanded mixture, water and wheat bran
Amount is than being 11-17:8-12:2-3, to sterilizing 10-20 minutes at 123 DEG C of mixed liquor, is cooled to 25-50 DEG C;
(3.4) Lactobacillus plantarum nutrient solution, the 0.67%-1.25% of 2%-2.5% volumes are added in the mixed liquor after cooling
The mycotic culture liquid of the bacillus nutrient solution of volume and 0.67%-1.25% balls product, at 30-50 DEG C, anaerobic condition fermentation 40-
80 hours, sterilize 10-20 minutes at 123 DEG C after fermentation;
(3.5) zymotic fluid after step (3.4) sterilizing is down to after room temperature and adds phosphoric acid, adjust pH to 4.8, centrifugation to separate
Obtain free oil, emulsion layer and residue;
(3.6) by the isolated free oil of emulsion layer;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains maize germ oil.
(4) onion oil, maize germ oil, resveratrol micro mist and Punica granatum L. polyphenol are mixed by weight, as reducing blood lipid
Composition.
Maize germ oil yield is 93.2-93.7%, with linoleic in high effective liquid chromatography for measuring maize germ oil
Content is 55%.Specific maize germ oil preparation method of the invention, purity is high, and the cell-wall breaking ratio in maize germ increases, and
Contact area is increased during the fermentation, and the yield of maize germ oil is improve by a relatively large margin.
Present invention onion oil prepares the composition of reducing blood lipid multiple combination:
1st, onion oil 54, maize germ oil 30, resveratrol micro mist 15.15, Punica granatum L. polyphenol 0.85.
2nd, onion oil 60, maize germ oil 25, resveratrol micro mist 14.15, Punica granatum L. polyphenol 0.85.
3rd, onion oil 60, maize germ oil 26.15, resveratrol micro mist 13, Punica granatum L. polyphenol 0.85.
4th, onion oil 40, maize germ oil 35, resveratrol micro mist 24.15, Punica granatum L. polyphenol 0.85.
5th, onion oil 49.5, maize germ oil 25, resveratrol micro mist 25, Punica granatum L. polyphenol 0.85.
6th, onion oil 50, maize germ oil 35, resveratrol micro mist 14.15, Punica granatum L. polyphenol 0.85.
Embodiment 1, the preparation method that the composition of reducing blood lipid is prepared with onion oil comprises the steps:
(1) resveratrol micro mist 15.15 is taken by weight and Punica granatum L. polyphenol 0.85 is standby;
(2) onion oil is prepared:
(2.1) the fresh onions of 1000g are taken, crushing is centrifuged to obtain 410ml Conditions of Onion Juice;
(2.2) 205g sodium carbonate is added in Conditions of Onion Juice, the ethanol or absolute ethyl alcohol of 410ml 95% is added after dissolving, mixed
Aqueous two-phase system is formed afterwards, and phase and phase on 400ml aqueous two-phase extractions under 420ml aqueous two-phase extractions are obtained after standing;
(2.3) phase in taking-up, reduced pressure concentration, rotary evaporation, obtain rough onion oil 95ml;
(2.4) phase is removed, with 420ml chloroforms and ethanol volume 5:1 mixed liquor is extracted 40 minutes, and 430ml is obtained after standing
Phase under upper phase and 400ml;
(2.5) upper reduced pressure concentration, rotary evaporation, recycling design at 20-70 DEG C in step (2.4), obtains rough onion
Oily 12ml;
(2.6) the rough onion oil obtained in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and ethanol
Mixed solvent is eluted four times, and chloroform is followed successively by 9 with the volume ratio of ethanol:1、6:1、3:1、2.3:1, to gather the stream of onion oil
Part;
(2.7) by the stream part containing onion oil in 20-70 DEG C of decompression rotary evaporation, recycling design is dehydrated desalination, is refined
Onion oil 49g, purity is 99.85%, yield 4.9%;
(3) maize germ oil is prepared:
(3.1) take and be well mixed after 15kg maize germs and the crushing of 2kg corn peels, obtain compound;
(3.2) take 1.36kg water to add in compound, insert after stirring in extruder, to maize germ and maize seed
Skin is carried out after extrusion, obtains expanded mixture;
(3.3) take the expanded mixture in step (3.2) and be well mixed with 12kg water and 3kg wheat bran and obtain mixed liquor, it is swollen
Change mixture, to sterilizing 20 minutes at 123 DEG C of mixed liquor, be cooled to 50 DEG C;
(3.4) 500ml Lactobacillus plantarum nutrient solutions, 250ml bacillus nutrient solutions are added in the mixed liquor after cooling
And 250ml mycotic culture liquid, at 50 DEG C, anaerobic condition ferments 80 hours, sterilizes 20 minutes at 123 DEG C after fermentation;
(3.5) zymotic fluid after step (3.4) sterilizing is down to after room temperature and adds phosphoric acid, adjust pH to 4.8, centrifugation to separate
Obtain free oil, emulsion layer and residue;
(3.6) by the isolated free oil of emulsion layer;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains maize germ oil 7kg;
Maize germ oil yield is 99.9%, and purity is 99.94%;Content with high effective liquid chromatography for measuring free oil Linoleic acid is
55%;
(4) onion oil 54, maize germ oil 30, resveratrol micro mist 15.15 and Punica granatum L. polyphenol 0.85 are mixed by weight
, as blood-fat reducing composition, pack, every bag of 10g.
Embodiment 2, the preparation method that the composition of reducing blood lipid is prepared with onion oil comprises the steps:
(1) resveratrol micro mist 14.15 is taken by weight and Punica granatum L. polyphenol 0.85 is standby;
(2) onion oil is prepared:
(2.1) the fresh onions of 500g are taken, crushing is centrifuged to obtain Conditions of Onion Juice 200ml;
(2.2) 62g sodium carbonate is added in Conditions of Onion Juice, the ethanol or absolute ethyl alcohol of 200ml 95% is added after dissolving, be mixed
After form aqueous two-phase system, after standing phase mutually and on 210ml aqueous two-phase extractions under 190ml aqueous two-phase extractions;
(2.3) phase in taking-up, reduced pressure concentration, rotary evaporation, obtain rough onion oil 43ml;
(2.4) phase is removed, with 190ml chloroforms and ethanol volume ratio 5:1 mixed liquor is extracted 30 minutes, after standing
Phase under the upper phases of 200ml and 180ml;
(2.5) upper reduced pressure concentration, rotary evaporation, recycling design at 20-70 DEG C in step (2.4), obtains rough onion
Oily 2ml;
(2.6) the rough onion oil obtained in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and ethanol
Mixed solvent is eluted four times, and chloroform is followed successively by 9 with the volume ratio of ethanol:1、6:1、3:1、2.3:1, to gather the stream of onion oil
Part;
(2.7) by the stream part containing onion oil in 20-70 DEG C of decompression rotary evaporation, recycling design is dehydrated desalination, is refined
Onion oil 19.55g, purity is 99.81%, yield 3.91%.
(3) maize germ oil is prepared:
(3.1) take and be well mixed after 10kg maize germs and the crushing of 1kg corn peels, obtain compound;
(3.2) take 0.77kg water to add in compound, insert after stirring in extruder, to maize germ and maize seed
Skin is carried out after extrusion, obtains expanded mixture;
(3.3) the expanded mixture taken in step (3.2) is well mixed and obtains mixed liquor with 8kg water and 2kg wheat bran, to mixed
Close and sterilized 10-20 minutes at 123 DEG C of liquid, be cooled to 25 DEG C;
(3.4) 300ml Lactobacillus plantarum nutrient solutions, 100ml bacillus nutrient solutions are added in the mixed liquor after cooling
And 100ml mycotic culture liquid, at 30 DEG C, anaerobic condition ferments 40 hours, sterilizes 10 minutes at 123 DEG C after fermentation;
(3.5) zymotic fluid after step (3.4) sterilizing is down to after room temperature and adds phosphoric acid, adjust pH to 4.8, centrifugation to separate
Obtain free oil, emulsion layer and residue;
(3.6) by the isolated free oil of emulsion layer;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains 4.68kg maizes
Bud oil;Maize germ oil yield is 99.7%, and purity is 99.92%.With high effective liquid chromatography for measuring free oil Linoleic acid
Content is 55%,
(4) onion oil 60, maize germ oil 25, resveratrol micro mist 14.15, Punica granatum L. polyphenol 0.85 are mixed by weight
, as blood-fat reducing composition, pack, every bag of 10g.
Embodiment 3, the preparation method that the composition of reducing blood lipid is prepared with onion oil comprises the steps:
(1) resveratrol micro mist 13- 25 is taken by weight and Punica granatum L. polyphenol 0.85 is standby;
(2) onion oil is prepared:
(2.1) the fresh onions of 750g are taken, crushing is centrifuged to obtain 305ml Conditions of Onion Juice;
(2.2) 85g sodium carbonate is added in Conditions of Onion Juice, the ethanol or absolute ethyl alcohol of 305ml 95% is added after dissolving, be mixed
After form aqueous two-phase system, after standing phase mutually and on 305ml aqueous two-phase extractions under 305ml aqueous two-phase extractions;
(2.3) phase in taking-up, reduced pressure concentration, rotary evaporation, obtain rough onion oil 69ml;
(2.4) phase is removed, with 305ml chloroforms and ethanol volume ratio 5:1 mixed liquor extracts 30-40 minutes, after standing
Phase under the upper phases of 317ml and 290ml;
(2.5) upper reduced pressure concentration, rotary evaporation, recycling design at 20-70 DEG C in step (2.4), obtains rough onion
Oily 7ml;
(2.6) the rough onion oil obtained in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and ethanol
Mixed solvent is eluted four times, and chloroform is followed successively by 9 with the volume ratio of ethanol:1、6:1、3:1、2.3:1, to gather the stream of onion oil
Part;
(2.7) by the stream part containing onion oil in 20-70 DEG C of decompression rotary evaporation, recycling design is dehydrated desalination, is refined
Onion oil 32.2g, purity is 99.83%, yield 4.3%.
(3) maize germ oil is prepared:
(3.1) take and be well mixed after 12.5kg maize germs and the crushing of 1.5kg corn peels, obtain compound;
(3.2) 1kg that fetches water is added in compound, is inserted after stirring in extruder, to maize germ and corn peel
After carrying out extrusion, expanded mixture is obtained;
(3.3) the expanded mixture taken in step (3.2) is well mixed and obtains mixed liquor with 10kg water and 2.5kg wheat bran,
Expanded mixture, to sterilizing 15 minutes at 123 DEG C of mixed liquor, is cooled to 38 DEG C;
(3.4) 350ml Lactobacillus plantarum nutrient solutions, 200ml bacillus nutrient solutions are added in the mixed liquor after cooling
And 0250ml mycotic culture liquid, at 40 DEG C, anaerobic condition ferments 60 hours, sterilizes 15 minutes at 123 DEG C after fermentation;
(3.5) zymotic fluid after step (3.4) sterilizing is down to after room temperature and adds phosphoric acid, adjust pH to 4.8, centrifugation to separate
Obtain free oil, emulsion layer and residue;
(3.6) by the isolated free oil of emulsion layer;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains 5.87kg maize germs
Oil.Maize germ oil yield is 99.8%, and purity is 99.93%;With containing for high effective liquid chromatography for measuring free oil Linoleic acid
Measure as 55%;
(4) onion oil 49.5, maize germ oil 25, resveratrol micro mist 25 and Punica granatum L. polyphenol 0.85 are mixed by weight
, as blood-fat reducing composition, pack, every bag of 10g.
Resveratrol micro mist of the present invention is the method processing crushed using the higher-order of oscillation with commercially available sterling resveratrol
Make, the process for preparing micropowder of resveratrol may also be employed other well known micro mist preparation method and make.
Lipid-lowering test:
1st, materials and methods
1.1 material
Animal used as test takes Wisrer rats 90, rat body weight 150-180g, male and female half and half.
1.2 method
90 rats are divided into into two groups, one group is raised for high lipid food, one group for normal diet raising.High lipid food
Increase sterling cholesterol 10%, lard 12%, sucrose 3% on the basis of normal diet.After high lipid food group is fed 15 days, blood drawing is surveyed
Determine serum total cholesterol and triglyceride, be randomly divided into high fat group, clofibrate group, the present composition implement 1 group, implement 5 groups,
Onion oil group, the present composition component implemented 1 group, implement 5 groups, is admixed in high lipid food daily by 85mg/kg consumptions,
Clofibrate group is admixed in high lipid food by 3.2mg/kg consumptions, and onion oil group is admixed in high lipid food by 85mg/kg consumptions, each group
Rat, each group rat free water are fed respectively.Blood fat was weighed and surveyed per 10 days.After successive administration 40 days, rat, abdomen are put to death
Vena cave takes blood, determines T-CHOL, triglyceride, HDL-C and low density cholesterol with full automatic biochemical apparatus and contains
Amount.
2nd, experimental result
The serum TC of each group rat(Cholesterol)、TG(Triglyceride)、HDL-C(HDL-C)、LDL-C
(LDL-C)Content is shown in Table 1.Test result indicate that:The serum TC of high fat group rat, TG and LDL-C concentration are equal
Apparently higher than normal diet group, HDL-C concentration is significantly lower than normal diet group.The blood of two groups of feed group rats of the present composition
Clear TC, TG and LDL-C concentration is significantly lower than high fat group, HDL-C concentration apparently higher than high fat group, and than clofibrate group rat
Serum TC concentration it is low, TG, LDL-C and HDL-C concentration is relatively.Test result indicate that, the present composition has substantially
Effect for reducing blood fat, lipid-lowering effect is close with the lipid-lowering effect of clofibrate, and experimental result shows simultaneously, the present composition
Lipid-lowering effect is substantially better than the lipid-lowering effect of onion oil, and synergy is notable.
Table 1
△ P<0.05,△△p<0.01。