CN103948820A - Blood fat-reducing composition prepared from onion oil and preparation method for same - Google Patents
Blood fat-reducing composition prepared from onion oil and preparation method for same Download PDFInfo
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Abstract
The invention provides a blood fat-reducing composition prepared from onion oil and a preparation method for the same. The blood fat-reducing composition is composed of the following raw materials in parts by weight: 40-60 parts of onion oil, 25-35 parts of maize germ oil, 13-25 parts of resveratrol micro-powder and 0.85 part of pomegranate polyphenol. The preparation method comprises the following preparation steps: (1) taking resveratrol micro-powder and pomegranate polyphenol in parts by weight for later use; (2) preparing onion oil; (3) preparing maize germ oil; and (4) uniformly mixing onion oil, maize germ oil, resveratrol micro-powder and pomegranate polyphenol in parts by weight to obtain the blood fat-reducing composition. The blood fat-reducing composition provided by the invention is obtained by combining high-purity extracts in natural plants, obvious in blood fat-reducing effect, free from toxic and side effects, and convenient to carry and take.
Description
Technical field
The present invention relates to oil of Bulbus Allii Cepae, is a kind of composition and method of making the same of preparing blood fat reducing with oil of Bulbus Allii Cepae.
Background technology
Along with greatly enriching of material life, the crowd who suffers from hyperlipidemia increases year by year, and according to statistics, the rejuvenation of hyperlipidemia is in recent years also in progressively development.Because the Main Ingredients and Appearance in hyperlipidemia is triglyceride and cholesterol, in the time of Hyperlipidemia, easily cause atherosclerosis, finally cause cardiovascular and cerebrovascular disease.Therefore, along with popularizing of health education, people are improved a lot to the attention degree of hyperlipidemia, and the blood fat reducing of taking medicine is accepted by the patient of hyperlipidemia.But current commercially available hypolipidemic is mainly Western medicine and Chinese patent medicine, Western medicine lipid-lowering effect is obvious, but the internal organs such as Liver and kidney to human body have obvious side effect, and Chinese patent medicine needs long-term taking, and curative effect is more difficult to be determined.Disclosed antilipemic Chinese herbal medicine decoction in addition, need to do different selections according to different body constitution, meanwhile, the preparation complexity of decoction, effective slower.
Summary of the invention
The object of this invention is to provide a kind of composition and method of making the same of preparing blood fat reducing with oil of Bulbus Allii Cepae, it adopts the high-purity extract combination in natural plants, and lipid-lowering effect is obvious, has no side effect, and is easy to carry and takes.
The present invention for achieving the above object, be achieved through the following technical solutions: a kind of compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae, is made up of the raw material of following weight ratio: oil of Bulbus Allii Cepae 40-60, Fructus Maydis oil 25-35, resveratrol micropowder 13-25 and Punica granatum L. polyphenol 0.85.
Described a kind of compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae, is made up of the raw material of following weight ratio: oil of Bulbus Allii Cepae 54, Fructus Maydis oil 30, resveratrol micropowder 15.15, Punica granatum L. polyphenol 0.85.
Described a kind of compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae, is made up of the raw material of following weight ratio: oil of Bulbus Allii Cepae 60, Fructus Maydis oil 25, resveratrol micropowder 14.15, Punica granatum L. polyphenol 0.85.
Described a kind of preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae, comprises the steps:
(1) get by weight resveratrol micropowder and Punica granatum L. polyphenol for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get fresh Bulbus Allii Cepae, pulverize the centrifugal Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add sodium carbonate, sodium carbonate addition is the 1/8-1/2 that dissolves Sucus Allii Fistulosi volume, adds 95% ethanol or dehydrated alcohol after dissolving, after being mixed, forms aqueous two-phase system, and the addition of ethanol is identical with the volume of oil of Bulbus Allii Cepae;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae;
(2.4) take off phase, with chloroform and alcohol mixeding liquid extraction 30-40 minute, after leaving standstill, obtain upper and lower phase, the volume ratio 5:1 of chloroform and ethanol, mixed liquor is identical with lower phase volume;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae, purity is 99.85%;
(3) prepare Fructus Maydis oil:
(3.1) get Fructus Maydis and corn peel and pulverize rear mix homogeneously in the ratio of 10-15:1-2, obtain compound;
(3.2) add in compound by the 7-8% water intaking of compound gross weight, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) get expanded mixture, water and Testa Tritici mix homogeneously and obtain mixed liquor, the weight ratio of expanded mixture, water and Testa Tritici is 11-17:8-12:2-3, to sterilizing 10-20 minute at 123 DEG C of mixed liquors, is cooled to 25-50 DEG C;
(3.4) in cooled mixed liquor, add the Lactobacillus plantarum culture fluid of 2%-2.5% volume, bacillus cereus culture fluid and the long-pending mycotic culture liquid of 0.67%-1.25% ball of 0.67%-1.25% volume, at 30-50 DEG C, anaerobic condition fermentation 40-80 hour, after fermentation at 123 DEG C of sterilizing 10-20 minute;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains Fructus Maydis oil.
(4) by weight oil of Bulbus Allii Cepae, Fructus Maydis oil, resveratrol micropowder and Punica granatum L. polyphenol are mixed, be blood-fat reducing composition.
Described a kind of preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae, comprises the steps:
(1) get by weight resveratrol micropowder 15.15 and Punica granatum L. polyphenol 0.85 is for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get the fresh Bulbus Allii Cepae of 1000g, pulverize the centrifugal 410ml Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add 205g sodium carbonate, after dissolving, add ethanol or the dehydrated alcohol of 410ml 95%, after being mixed, form aqueous two-phase system, after leaving standstill phase mutually and on 400ml aqueous two-phase extraction under 420ml aqueous two-phase extraction;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae 95ml;
(2.4) take off phase, use the mixed liquor of 420ml chloroform and ethanol volume 5:1 to extract 40 minutes, after leaving standstill, obtain phase under the upper phase of 430ml and 400ml;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae 12ml;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae 49g, purity is 99.85%, yield 4.9%;
(3) prepare Fructus Maydis oil:
(3.1) get 15kg Fructus Maydis and 2kg corn peel and pulverize rear mix homogeneously, obtain compound;
(3.2) get 1.36kg water and add in compound, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) the expanded mixture of getting in step (3.2) is mixed homogeneously and is obtained mixed liquor with 12kg water and 3kg Testa Tritici, and expanded mixture, to sterilizing at 123 DEG C of mixed liquors 20 minutes, is cooled to 50 DEG C;
(3.4) in cooled mixed liquor, add 500ml Lactobacillus plantarum culture fluid, 250ml bacillus cereus culture fluid and 250ml mycotic culture liquid, at 50 DEG C, anaerobic condition fermentation 80 hours, after fermentation 123 DEG C of sterilizings 20 minutes;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains Fructus Maydis oil 7kg; Fructus Maydis oil yield is 99.9%, and purity is 99.94%; With linoleic content in high effective liquid chromatography for measuring free oil be 55%;
(4) by weight oil of Bulbus Allii Cepae 54, Fructus Maydis oil 30, resveratrol micropowder 15.15 and Punica granatum L. polyphenol 0.85 are mixed, be blood-fat reducing composition.
The scallion oil that dissolves using in compositions of the present invention is that a kind of special method obtains, the oil of Bulbus Allii Cepae obtaining due to the method for published extraction oil of Bulbus Allii Cepae has bad smell, color is darker, when high temperature process, the active ingredient of oil of Bulbus Allii Cepae loss is larger, the oil of Bulbus Allii Cepae that some solvent method is produced, the materials such as the pigment in solvent are easily residual, can not be used for human body long-term taking, when major effect curative effect and long-term taking, human body be had to harmful effect.The oil of Bulbus Allii Cepae of extracting with ad hoc approach of the present invention is without bad smell, and purity reaches 99. 85%, and without loss and the destruction of thermal sensitivity volatilization composition, and yield is high, and yield reaches 3.96-4.9%; The purity of the resveratrol micropowder using is 99.5%; Punica granatum L. polyphenol be extracted as known method, the Punica granatum L. polyphenol that requires commercially available purity to be greater than 85%; Fructus Maydis oil adopts ad hoc approach of the present invention to extract, and does cracking agent with corn peel, Fructus Maydis is had to phasic property good, does not produce pollution, the feature that cell wall breaking rate is high, and the purity of Fructus Maydis oil is 99.94%.Oil of Bulbus Allii Cepae in compositions of the present invention can reduce triglyceride and the cholesterol in serum, its amount that reduces triglyceride reaches the more than 2 times of nature slippage, the amount that reduces cholesterol reaches the more than 1.5 times of nature slippage, research shows, oil of Bulbus Allii Cepae is separately for blood fat reducing, its effect has obvious reduction than the chemicals such as Etofylline Clofibrate and clofibrate, but sulfur compound in oil of Bulbus Allii Cepae, the 60 multiple beneficial compositions such as flavonoid, in thering is effect for reducing blood fat, there is the stronger effect such as antioxidation and anti-platelet aggregation, further research shows, the content height of blood fat has reflected the level of lipid metabolism in body, and when tremulous pulse and venous capillary elasticity are when lower, the level of metabolism lipid reduces relatively, while particularly lacking linoleic acid in body, cholesterol will combine with satisfied fatty acid, there is dysbolismus, in blood vessel wall, precipitate, when platelet aggregation in blood, when antioxidant content is lower, all affect lipids contents.The present invention is aided with Fructus Maydis oil, resveratrol micropowder and Punica granatum L. polyphenol, in order to improve blood vessel elasticity, reduce satisfied fatty acid in blood vessel and be combined with cholesterol, contained antioxidant can catch and in free radical, anti-platelet aggregation.Zoopery shows: the present composition has obvious synergism, has the effect of obvious blood fat reducing.The present composition also can be eliminated endovascular harmful substance.The present composition all extracts in natural materials, has no side effect, and long-term taking has no adverse effects to human body.The present composition is made capsule for reducing blood fat, and service once every day sooner or later, and each 3g, 10 days is a course for the treatment of.Prepare material purity in compositions by method of the present invention high, can ensure to reduce the effect of blood fat, the raw material yield of extraction is high, is easy to suitability for industrialized production, and production process is without any pollution.
Brief description of the drawings
Fig. 1 is that the onion essential oil that the present invention makes detects through GC-MS, identifies more than the 60 total ion current figure that plant sulfur-containing compound.
Detailed description of the invention
A kind of compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae of the present invention, is made up of the raw material of following weight ratio: oil of Bulbus Allii Cepae 40-60, Fructus Maydis oil 25-35, resveratrol micropowder 13-25 and Punica granatum L. polyphenol 0.85.
A preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae, comprises the steps:
(1) get by weight resveratrol micropowder and Punica granatum L. polyphenol for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get fresh Bulbus Allii Cepae, pulverize the centrifugal Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add sodium carbonate, sodium carbonate addition is the 1/8-1/2 that dissolves Sucus Allii Fistulosi volume, adds 95% ethanol or dehydrated alcohol after dissolving, after being mixed, forms aqueous two-phase system, and the addition of ethanol is identical with the volume of oil of Bulbus Allii Cepae;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae;
(2.4) take off phase, with chloroform and alcohol mixeding liquid extraction 30-40 minute, after leaving standstill, obtain upper and lower phase, the volume ratio 5:1 of chloroform and ethanol, mixed liquor is identical with lower phase volume;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae, purity is 99.85%;
(3) prepare Fructus Maydis oil:
(3.1) get Fructus Maydis and corn peel and pulverize rear mix homogeneously in the ratio of 10-15:1-2, obtain compound;
(3.2) add in compound by the 7-8% water intaking of compound gross weight, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) get expanded mixture, water and Testa Tritici mix homogeneously and obtain mixed liquor, the weight ratio of expanded mixture, water and Testa Tritici is 11-17:8-12:2-3, to sterilizing 10-20 minute at 123 DEG C of mixed liquors, is cooled to 25-50 DEG C;
(3.4) in cooled mixed liquor, add the Lactobacillus plantarum culture fluid of 2%-2.5% volume, bacillus cereus culture fluid and the long-pending mycotic culture liquid of 0.67%-1.25% ball of 0.67%-1.25% volume, at 30-50 DEG C, anaerobic condition fermentation 40-80 hour, after fermentation at 123 DEG C of sterilizing 10-20 minute;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains Fructus Maydis oil.
(4) by weight oil of Bulbus Allii Cepae, Fructus Maydis oil, resveratrol micropowder and Punica granatum L. polyphenol are mixed, be blood-fat reducing composition.
Fructus Maydis oil yield is 93.2-93.7%, with the linoleic content in high effective liquid chromatography for measuring Fructus Maydis oil be 55%.The specific Fructus Maydis oil preparation method of the present invention, purity is high, and the cell wall breaking rate in Fructus Maydis increases, and has increased contact area during the fermentation, has improved by a relatively large margin the yield of Fructus Maydis oil.
The present invention has multiple combination by the compositions that oil of Bulbus Allii Cepae is prepared blood fat reducing:
1, oil of Bulbus Allii Cepae 54, Fructus Maydis oil 30, resveratrol micropowder 15.15, Punica granatum L. polyphenol 0.85.
2, oil of Bulbus Allii Cepae 60, Fructus Maydis oil 25, resveratrol micropowder 14.15, Punica granatum L. polyphenol 0.85.
3, oil of Bulbus Allii Cepae 60, Fructus Maydis oil 26.15, resveratrol micropowder 13, Punica granatum L. polyphenol 0.85.
4, oil of Bulbus Allii Cepae 40, Fructus Maydis oil 35, resveratrol micropowder 24.15, Punica granatum L. polyphenol 0.85.
5, oil of Bulbus Allii Cepae 49.5, Fructus Maydis oil 25, resveratrol micropowder 25, Punica granatum L. polyphenol 0.85.
6, oil of Bulbus Allii Cepae 50, Fructus Maydis oil 35, resveratrol micropowder 14.15, Punica granatum L. polyphenol 0.85.
Embodiment 1, the preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae comprises the steps:
(1) get by weight resveratrol micropowder 15.15 and Punica granatum L. polyphenol 0.85 is for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get the fresh Bulbus Allii Cepae of 1000g, pulverize the centrifugal 410ml Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add 205g sodium carbonate, after dissolving, add ethanol or the dehydrated alcohol of 410ml 95%, after being mixed, form aqueous two-phase system, after leaving standstill phase mutually and on 400ml aqueous two-phase extraction under 420ml aqueous two-phase extraction;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae 95ml;
(2.4) take off phase, use the mixed liquor of 420ml chloroform and ethanol volume 5:1 to extract 40 minutes, after leaving standstill, obtain phase under the upper phase of 430ml and 400ml;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae 12ml;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae 49g, purity is 99.85%, yield 4.9%;
(3) prepare Fructus Maydis oil:
(3.1) get 15kg Fructus Maydis and 2kg corn peel and pulverize rear mix homogeneously, obtain compound;
(3.2) get 1.36kg water and add in compound, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) the expanded mixture of getting in step (3.2) is mixed homogeneously and is obtained mixed liquor with 12kg water and 3kg Testa Tritici, and expanded mixture, to sterilizing at 123 DEG C of mixed liquors 20 minutes, is cooled to 50 DEG C;
(3.4) in cooled mixed liquor, add 500ml Lactobacillus plantarum culture fluid, 250ml bacillus cereus culture fluid and 250ml mycotic culture liquid, at 50 DEG C, anaerobic condition fermentation 80 hours, after fermentation 123 DEG C of sterilizings 20 minutes;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains Fructus Maydis oil 7kg; Fructus Maydis oil yield is 99.9%, and purity is 99.94%; With linoleic content in high effective liquid chromatography for measuring free oil be 55%;
(4) by weight oil of Bulbus Allii Cepae 54, Fructus Maydis oil 30, resveratrol micropowder 15.15 and Punica granatum L. polyphenol 0.85 are mixed, be blood-fat reducing composition, pack, every bag of 10g.
Embodiment 2, the preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae comprises the steps:
(1) get by weight resveratrol micropowder 14.15 and Punica granatum L. polyphenol 0.85 is for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get the fresh Bulbus Allii Cepae of 500g, pulverize the centrifugal Conditions of Onion Juice 200ml of obtaining;
(2.2) in Conditions of Onion Juice, add 62g sodium carbonate, after dissolving, add ethanol or the dehydrated alcohol of 200ml 95%, after being mixed, form aqueous two-phase system, after leaving standstill phase mutually and on 210ml aqueous two-phase extraction under 190ml aqueous two-phase extraction;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae 43ml;
(2.4) take off phase, use the mixed liquor of 190ml chloroform and ethanol volume ratio 5:1 to extract 30 minutes, after leaving standstill, obtain phase under the upper phase of 200ml and 180ml;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae 2ml;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae 19.55g, purity is 99.81%, yield 3.91%.
(3) prepare Fructus Maydis oil:
(3.1) get 10kg Fructus Maydis and 1kg corn peel and pulverize rear mix homogeneously, obtain compound;
(3.2) get 0.77kg water and add in compound, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) the expanded mixture of getting in step (3.2) is mixed homogeneously and is obtained mixed liquor with 8kg water and 2kg Testa Tritici, to sterilizing 10-20 minute at 123 DEG C of mixed liquors, is cooled to 25 DEG C;
(3.4) in cooled mixed liquor, add 300ml Lactobacillus plantarum culture fluid, 100ml bacillus cereus culture fluid and 100ml mycotic culture liquid, at 30 DEG C, anaerobic condition fermentation 40 hours, after fermentation 123 DEG C of sterilizings 10 minutes;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains 4.68kg Fructus Maydis oil; Fructus Maydis oil yield is 99.7%, and purity is 99.92%.With linoleic content in high effective liquid chromatography for measuring free oil be 55%,
(4) by weight oil of Bulbus Allii Cepae 60, Fructus Maydis oil 25, resveratrol micropowder 14.15, Punica granatum L. polyphenol 0.85 are mixed, be blood-fat reducing composition, pack, every bag of 10g.
Embodiment 3, the preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae comprises the steps:
(1) get by weight resveratrol micropowder 25 and Punica granatum L. polyphenol 0.85 is for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get the fresh Bulbus Allii Cepae of 750g, pulverize the centrifugal 305ml Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add 85g sodium carbonate, after dissolving, add ethanol or the dehydrated alcohol of 305ml 95%, after being mixed, form aqueous two-phase system, after leaving standstill phase mutually and on 305ml aqueous two-phase extraction under 305ml aqueous two-phase extraction;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae 69ml;
(2.4) take off phase, use 305ml chloroform and ethanol volume ratio 5:1 mixed liquor extraction 30-40 minute, after leaving standstill, obtain phase under the upper phase of 317ml and 290ml;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae 7ml;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae 32.2g, purity is 99.83%, yield 4.3%.
(3) prepare Fructus Maydis oil:
(3.1) get 12.5kg Fructus Maydis and 1.5kg corn peel and pulverize rear mix homogeneously, obtain compound;
(3.2) water intaking 1kg adds in compound, inserts in extruder after stirring, and Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) the expanded mixture of getting in step (3.2) is mixed homogeneously and is obtained mixed liquor with 10kg water and 2.5kg Testa Tritici, and expanded mixture, to sterilizing at 123 DEG C of mixed liquors 15 minutes, is cooled to 38 DEG C;
(3.4) in cooled mixed liquor, add 350ml Lactobacillus plantarum culture fluid, 200ml bacillus cereus culture fluid and 0250ml mycotic culture liquid, at 40 DEG C, anaerobic condition fermentation 60 hours, after fermentation 123 DEG C of sterilizings 15 minutes;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains 5.87kg Fructus Maydis oil.Fructus Maydis oil yield is 99.8%, and purity is 99.93%; With linoleic content in high effective liquid chromatography for measuring free oil be 55%;
(4) by weight oil of Bulbus Allii Cepae 49.5, Fructus Maydis oil 25, resveratrol micropowder 25 and Punica granatum L. polyphenol 0.85 are mixed, be blood-fat reducing composition, pack, every bag of 10g.
Resveratrol micropowder of the present invention is to be processed into by the method that commercially available sterling resveratrol adopts the higher-order of oscillation to pulverize, and the process for preparing micropowder of resveratrol also can adopt other known micropowder preparation method to make.
Lipid-lowering test:
1, materials and methods
1.1 material
Laboratory animal is got 90 of Wisrer rats, rat body weight 150-180g, male and female half and half.
1.2 method
90 rats are divided into two groups, and one group is used for fed with high, raises for one group for normal diet.High lipid food increases sterling cholesterol 10%, Adeps Sus domestica 12%, sucrose 3% on normal diet basis.High lipid food group was fed after 15 days, serum total cholesterol and triglyceride are measured in blood drawing, being divided at random high fat group, clofibrate group, the present composition implements 1 group, implements 5 groups, Bulbus Allii Cepae line of oils, the present composition is with implementing 1 group, implementing the component of 5 groups, admix in high lipid food every day by 85mg/kg consumption, and clofibrate group is admixed in high lipid food by 3.2mg/kg consumption, and Bulbus Allii Cepae line of oils is admixed in high lipid food by 85mg/kg consumption, feed respectively rat for each group, respectively organize rat and freely drink water.Within every 10 days, weigh and survey blood fat.After successive administration 40 days, put to death rat, abdominal vein is got blood, measures T-CHOL, triglyceride, HDL-C and low density cholesterol content with full automatic biochemical apparatus.
2, experimental result
Serum TC (cholesterol), the TG(triglyceride of each group rat), HDL-C(HDL-C), LDL-C(low-density lipoprotein cholesterol) content is in table 1.Experimental result shows: serum TC, TG and the LDL-C concentration of high fat group rat are all apparently higher than normal diet group, and HDL-C concentration is starkly lower than normal diet group.Serum TC, TG and the LDL-C concentration of two groups of feedstuff group rats of the present composition are all starkly lower than high fat group, and HDL-C concentration is apparently higher than high fat group, and lower than the serum TC concentration of clofibrate group rat, and TG, LDL-C and HDL-C concentration are all more approaching.Experimental result shows, the present composition has obvious effect for reducing blood fat, and the lipid-lowering effect of lipid-lowering effect and clofibrate is close, and experimental result shows simultaneously, and the lipid-lowering effect of the present composition is obviously better than the lipid-lowering effect of oil of Bulbus Allii Cepae, and synergism is remarkable.
Table 1
△?P<0.05,△△p<0.01。
Claims (5)
1. a compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae, is characterized in that: the raw material by following weight ratio forms: oil of Bulbus Allii Cepae 40-60, Fructus Maydis oil 25-35, resveratrol micropowder 13-25 and Punica granatum L. polyphenol 0.85.
2. a kind of compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae according to claim 1, is characterized in that: the raw material by following weight ratio forms: oil of Bulbus Allii Cepae 54, Fructus Maydis oil 30, resveratrol micropowder 15.15, Punica granatum L. polyphenol 0.85.
3. a kind of compositions of preparing blood fat reducing with oil of Bulbus Allii Cepae according to claim 1, is characterized in that: the raw material by following weight ratio forms: oil of Bulbus Allii Cepae 60, Fructus Maydis oil 25, resveratrol micropowder 14.15, Punica granatum L. polyphenol 0.85.
4. a kind of preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae described in claim 1-3 any one, is characterized in that: comprise the steps:
(1) get by weight resveratrol micropowder and Punica granatum L. polyphenol for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get fresh Bulbus Allii Cepae, pulverize the centrifugal Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add sodium carbonate, sodium carbonate addition is the 1/8-1/2 that dissolves Sucus Allii Fistulosi volume, adds 95% ethanol or dehydrated alcohol after dissolving, after being mixed, forms aqueous two-phase system, and the addition of ethanol is identical with the volume of oil of Bulbus Allii Cepae;
(2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae;
(2.4) take off phase, with chloroform and alcohol mixeding liquid extraction 30-40 minute, after leaving standstill, obtain upper and lower phase, the volume ratio 5:1 of chloroform and ethanol, mixed liquor is identical with lower phase volume;
(2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae;
(2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae;
(2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae, purity is 99.85%;
(3) prepare Fructus Maydis oil:
(3.1) get Fructus Maydis and corn peel and pulverize rear mix homogeneously in the ratio of 10-15:1-2, obtain compound;
(3.2) add in compound by the 7-8% water intaking of compound gross weight, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) get expanded mixture, water and Testa Tritici mix homogeneously and obtain mixed liquor, the weight ratio of expanded mixture, water and Testa Tritici is 11-17:8-12:2-3, to sterilizing 10-20 minute at 123 DEG C of mixed liquors, is cooled to 25-50 DEG C;
(3.4) in cooled mixed liquor, add the Lactobacillus plantarum culture fluid of 2%-2.5% volume, bacillus cereus culture fluid and the long-pending mycotic culture liquid of 0.67%-1.25% ball of 0.67%-1.25% volume, at 30-50 DEG C, anaerobic condition fermentation 40-80 hour, after fermentation at 123 DEG C of sterilizing 10-20 minute;
(3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue;
(3.6) emulsion layer is separated and obtains free oil;
(3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains Fructus Maydis oil;
(4) by weight oil of Bulbus Allii Cepae, Fructus Maydis oil, resveratrol micropowder and Punica granatum L. polyphenol are mixed, be blood-fat reducing composition.
5. a kind of preparation method of preparing the compositions of blood fat reducing with oil of Bulbus Allii Cepae according to claim 4, is characterized in that: comprise the steps:
(1) get by weight resveratrol micropowder 15.15 and Punica granatum L. polyphenol 0.85 is for subsequent use;
(2) prepare oil of Bulbus Allii Cepae:
(2.1) get the fresh Bulbus Allii Cepae of 1000g, pulverize the centrifugal 410ml Conditions of Onion Juice that obtains;
(2.2) in Conditions of Onion Juice, add 205g sodium carbonate, after dissolving, add ethanol or the dehydrated alcohol of 410ml 95%, after being mixed, form aqueous two-phase system, after leaving standstill phase mutually and on 400ml aqueous two-phase extraction under 420ml aqueous two-phase extraction; (2.3) phase in taking-up, concentrating under reduced pressure, rotary evaporation, obtains rough oil of Bulbus Allii Cepae 95ml; (2.4) take off phase, use the mixed liquor of 420ml chloroform and ethanol volume 5:1 to extract 40 minutes, after leaving standstill, obtain phase under the upper phase of 430ml and 400ml; (2.5) in step (2.4), go up concentrating under reduced pressure at 20-70 DEG C, rotary evaporation, reclaims solvent, obtains rough oil of Bulbus Allii Cepae 12ml; (2.6) the rough oil of Bulbus Allii Cepae obtaining in step (2.3), step (2.5) is inserted in chromatographic column, with chloroform and alcohol mixed solvent eluting four times, the volume ratio of chloroform and ethanol is followed successively by 9:1,6:1,3:1,2.3:1, to gather stream part of oil of Bulbus Allii Cepae; (2.7) by containing stream part of oil of Bulbus Allii Cepae at 20-70 DEG C of decompression rotary evaporation, reclaim solvent, dehydration desalination, obtains refining oil of Bulbus Allii Cepae 49g, purity is 99.85%, yield 4.9%;
(3) prepare Fructus Maydis oil: (3.1) get 15kg Fructus Maydis and 2kg corn peel is pulverized rear mix homogeneously, obtain compound;
(3.2) get 1.36kg water and add in compound, after stirring, insert in extruder, Fructus Maydis and corn peel are carried out, after extruding puffing, obtaining expanded mixture;
(3.3) the expanded mixture of getting in step (3.2) is mixed homogeneously and is obtained mixed liquor with 12kg water and 3kg Testa Tritici, and expanded mixture, to sterilizing at 123 DEG C of mixed liquors 20 minutes, is cooled to 50 DEG C; (3.4) in cooled mixed liquor, add 500ml Lactobacillus plantarum culture fluid, 250ml bacillus cereus culture fluid and 250ml mycotic culture liquid, at 50 DEG C, anaerobic condition fermentation 80 hours, after fermentation 123 DEG C of sterilizings 20 minutes; (3.5) add phosphoric acid after the fermentation liquid after step (3.4) sterilizing is down to room temperature, adjust pH to 4.8, centrifugal, separate and obtain free oil, emulsion layer and residue; (3.6) emulsion layer is separated and obtains free oil; (3.7) free oil in the free oil in step (3.5) and step (3.6) is merged and obtains Fructus Maydis oil 7kg; Fructus Maydis oil yield is 99.9%, and purity is 99.94%; With linoleic content in high effective liquid chromatography for measuring free oil be 55%;
(4) by weight oil of Bulbus Allii Cepae 54, Fructus Maydis oil 30, resveratrol micropowder 15.15 and Punica granatum L. polyphenol 0.85 are mixed, be blood-fat reducing composition.
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| CN101481722A (en) * | 2009-01-15 | 2009-07-15 | 刘建华 | Production method of compound oil special for fermentation |
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| CN101481722A (en) * | 2009-01-15 | 2009-07-15 | 刘建华 | Production method of compound oil special for fermentation |
| CN102511571A (en) * | 2011-12-20 | 2012-06-27 | 金利油脂（苏州）有限公司 | Sunflower seed lipid-lowering blend oil |
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