CN103940791A - 用荧光显微镜观测样本的方法 - Google Patents
用荧光显微镜观测样本的方法 Download PDFInfo
- Publication number
- CN103940791A CN103940791A CN201410027787.0A CN201410027787A CN103940791A CN 103940791 A CN103940791 A CN 103940791A CN 201410027787 A CN201410027787 A CN 201410027787A CN 103940791 A CN103940791 A CN 103940791A
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- CN
- China
- Prior art keywords
- sample
- exciting light
- carbon
- light
- irradiation
- Prior art date
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- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 39
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 39
- 238000010521 absorption reaction Methods 0.000 claims abstract description 17
- 230000004807 localization Effects 0.000 claims abstract description 11
- 238000003384 imaging method Methods 0.000 claims abstract 3
- 239000012528 membrane Substances 0.000 claims description 9
- 239000011248 coating agent Substances 0.000 claims description 8
- 238000000576 coating method Methods 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 7
- 238000000799 fluorescence microscopy Methods 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 6
- 230000001678 irradiating effect Effects 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 230000008093 supporting effect Effects 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims 2
- 230000005855 radiation Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 48
- 238000010438 heat treatment Methods 0.000 abstract description 7
- 238000000386 microscopy Methods 0.000 abstract description 7
- 238000005286 illumination Methods 0.000 abstract description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 230000001154 acute effect Effects 0.000 description 5
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 239000011889 copper foil Substances 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 241000479842 Pella Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000003319 supportive effect Effects 0.000 description 2
- 238000003325 tomography Methods 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13152215.3A EP2757402B1 (en) | 2013-01-22 | 2013-01-22 | Method of observing samples with a fluorescent microscope |
| EP13152215.3 | 2013-01-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103940791A true CN103940791A (zh) | 2014-07-23 |
Family
ID=47715843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410027787.0A Pending CN103940791A (zh) | 2013-01-22 | 2014-01-22 | 用荧光显微镜观测样本的方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20140203191A1 (https=) |
| EP (1) | EP2757402B1 (https=) |
| JP (1) | JP6262543B2 (https=) |
| CN (1) | CN103940791A (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855567A (zh) * | 2019-10-16 | 2020-10-30 | 中国科学院物理研究所 | 一种实现光学智能聚焦的透射电镜系统及方法 |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2824445B1 (en) | 2013-07-08 | 2016-03-02 | Fei Company | Charged-particle microscopy combined with raman spectroscopy |
| SE537103C2 (sv) * | 2013-12-16 | 2015-01-07 | Per Fogelstrand | System och metod för fluorescensmikroskopi med detektering av ljusemission från multipla fluorokromer |
| US11101104B2 (en) * | 2019-08-30 | 2021-08-24 | Fei Company | Multi modal cryo compatible GUID grid |
| CN114667586B (zh) * | 2019-10-04 | 2025-07-01 | 米特根有限公司 | 用于低温电子显微术的样品支架和样品冷却系统 |
| WO2022256588A1 (en) * | 2021-06-04 | 2022-12-08 | Mitegen, Llc | Sample supports for cryo-electron microscopy |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050059681A1 (en) * | 2001-10-09 | 2005-03-17 | Christopher Cremer | Far field light microscopical method system and computer program product for analysing at least one object having a subwavelength size |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE59805560D1 (de) | 1997-01-13 | 2002-10-24 | Daniel Studer | Probenhalter für wasserhaltige Proben sowie Verfahren zu deren Verwendung |
| EP1207414B1 (en) * | 1997-10-29 | 2016-05-04 | Motic China Group Co., Ltd. | Apparatus and methods relating to spatially light modulated microscopy |
| NL1017669C2 (nl) | 2001-03-22 | 2002-09-24 | Univ Maastricht | Inrichting voor het vervaardigen van preparaten voor een cryo-elektronenmicroscoop. |
| US6687035B2 (en) * | 2001-06-07 | 2004-02-03 | Leica Microsystems Heildelberg Gmbh | Method and apparatus for ROI-scan with high temporal resolution |
| NL1023717C2 (nl) | 2003-06-20 | 2004-12-21 | Fei Co | Preparaatdrager voor het dragen van een met een elektronenbundel te doorstralen preparaat. |
| JP2005216645A (ja) * | 2004-01-29 | 2005-08-11 | Jeol Ltd | 透過電子顕微鏡 |
| JP4425098B2 (ja) | 2004-09-06 | 2010-03-03 | 浜松ホトニクス株式会社 | 蛍光顕微鏡および蛍光相関分光解析装置 |
| US7355696B2 (en) * | 2005-02-01 | 2008-04-08 | Arryx, Inc | Method and apparatus for sorting cells |
| JP2006292421A (ja) * | 2005-04-06 | 2006-10-26 | Sharp Corp | 蛍光検出装置 |
| EP1863066A1 (en) | 2006-05-29 | 2007-12-05 | FEI Company | Sample carrier and sample holder |
| EP1953791A1 (en) * | 2007-02-05 | 2008-08-06 | FEI Company | Apparatus for observing a sample with a particle beam and an optical microscope |
| EP1998165A1 (en) * | 2007-06-01 | 2008-12-03 | The European Community, represented by the European Commission | Method of fluorescence imaging |
-
2013
- 2013-01-22 EP EP13152215.3A patent/EP2757402B1/en active Active
-
2014
- 2014-01-20 JP JP2014007377A patent/JP6262543B2/ja active Active
- 2014-01-21 US US14/160,135 patent/US20140203191A1/en not_active Abandoned
- 2014-01-22 CN CN201410027787.0A patent/CN103940791A/zh active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050059681A1 (en) * | 2001-10-09 | 2005-03-17 | Christopher Cremer | Far field light microscopical method system and computer program product for analysing at least one object having a subwavelength size |
Non-Patent Citations (1)
| Title |
|---|
| BRIEGEL等: "Chapter Thirteen: correlated light and electron cryo-microscope", 《METHODS IN ENZYMOLOGY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855567A (zh) * | 2019-10-16 | 2020-10-30 | 中国科学院物理研究所 | 一种实现光学智能聚焦的透射电镜系统及方法 |
| CN111855567B (zh) * | 2019-10-16 | 2021-07-20 | 中国科学院物理研究所 | 一种实现光学智能聚焦的透射电镜系统及方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6262543B2 (ja) | 2018-01-17 |
| JP2014142639A (ja) | 2014-08-07 |
| EP2757402A1 (en) | 2014-07-23 |
| US20140203191A1 (en) | 2014-07-24 |
| EP2757402B1 (en) | 2016-03-30 |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140723 |
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| WD01 | Invention patent application deemed withdrawn after publication |