CN103911286A - Sterile charging device of animal cell suspension culture bioreactor - Google Patents
Sterile charging device of animal cell suspension culture bioreactor Download PDFInfo
- Publication number
- CN103911286A CN103911286A CN201410118596.5A CN201410118596A CN103911286A CN 103911286 A CN103911286 A CN 103911286A CN 201410118596 A CN201410118596 A CN 201410118596A CN 103911286 A CN103911286 A CN 103911286A
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- CN
- China
- Prior art keywords
- feed pipe
- steam
- charging device
- pipeline
- suspension culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention belongs to the technical field of biology and relates to a sterile charging device of an animal cell suspension culture bioreactor. The sterile charging device comprises a feed pipeline, a steam pipeline, an exhaust pipeline, a liquid filter and a bioreactor; a raw material feeding valve, the liquid filter, a filtered material feeding valve and the bioreactor are orderly arranged on the feed pipeline in the feeding direction; the feed pipeline is divided into a front feed pipeline, a middle feed pipeline and a rear feed pipeline; the steam pipeline is connected to the feed pipeline between the raw material feeding valve and the liquid filter; branch pipelines communicated with the exhaust pipeline are connected to the front end and rear end of the middle feed pipeline respectively, and an exhaust valve and a drainage valve are arranged on the branch pipelines respectively. The sterile charging device of the animal cell suspension culture bioreactor is capable of saving energy sources and convenient for digital control.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of germ-free charging device of zooblast suspension culture bio-reactor.
Background technology
At present in the animal cell culture process of factory, must be strict with the requirement that environment in bio-reactor meets cell proliferation, reduce the impact of the materials such as extraneous bacterium on its environment as far as possible.So must to bio-reactor with and charging system carry out sterilising treatment.
The sterilization method that the existing charging system to bio-reactor adopts is generally high-temp steam sterilizing, pass into charging system by high-temperature steam, and maintain certain pressure and for some time, the sterilising plant of the charging system of bio-reactor is within the time period of sterilizing at present, need to there is high-temperature steam to pass into always, pass into high-temperature steam on one side, discharge high-temperature steam on one side, caused the waste of the energy.
Summary of the invention
Technical problem to be solved by this invention is, provide a kind of can save energy, the germ-free charging device of simple in structure, the zooblast suspension culture bio-reactor of being convenient to Digital Control.
The germ-free charging device of zooblast suspension culture bio-reactor of the present invention, comprise feed pipe, steam-pipe, gas exhaust duct, liquid filter, bio-reactor, described feed pipe is along being provided with in order raw material material inlet valve, liquid filter, filter material material inlet valve, bio-reactor on feedstock direction; Feed pipe is divided into anterior feed pipe, middle part feed pipe and rear-fed pipeline by described raw material material inlet valve and filter material material inlet valve; On feed pipe between described raw material material inlet valve and liquid filter, be connected with steam-pipe; On described steam-pipe, be provided with steam control valve; The front-end and back-end of described middle part feed pipe are connected with respectively the bypass line that is communicated to gas exhaust duct, on described branch pipeline, are respectively arranged with vent valve and drain valve.
The rear end of described middle part feed pipe is provided with two bypass line.
In described bypass line, be also provided with.
On described liquid filter, be provided with tensimeter.
The invention has the advantages that: in bypass line, be provided with drain valve, the built-in band temperature sense of drain valve diaphragm, reaches 121 while spending in temperature, and the effect that keeps sterilising temp is played in the discharge of steam off.When equitemperature is spent lower than 121, then the discharge of automatically opening steam, pass into steam.Play energy-conservation effect.In bypass line, be provided with temperature sensor, can Real-Time Monitoring sterilising temp.Whole device is also convenient to Digital Control.
Accompanying drawing explanation
Fig. 1 is embodiment of the present invention structural representation.
Embodiment
As shown in the figure, a kind of germ-free charging device of zooblast suspension culture bio-reactor, comprise feed pipe 1, steam-pipe 2, gas exhaust duct 3, liquid filter 4, bio-reactor 5, described feed pipe 1 is along being provided with in order raw material material inlet valve 6, liquid filter 4, filter material material inlet valve 7, bio-reactor 5 on feedstock direction; Feed pipe 1 is divided into anterior feed pipe, middle part feed pipe and rear-fed pipeline by described raw material material inlet valve 6 and filter material material inlet valve 7; On feed pipe 1 between described raw material material inlet valve 6 and liquid filter 4, be connected with steam-pipe 2; On described steam-pipe 2, be provided with steam diaphragm valve; The front-end and back-end of described middle part feed pipe are connected with respectively the bypass line 13 that is communicated to gas exhaust duct 3, are respectively arranged with vent valve 11 and drain valve 9 on described branch pipeline 13.
The rear end of described middle part feed pipe is provided with two bypass line 13.
In described bypass line 13, be also provided with temperature sensor 8.
On described liquid filter 4, be provided with tensimeter 12.
Working process is as follows: open the steam diaphragm valve on steam-pipe 2, close raw material material inlet valve 6 and filter material material inlet valve 7, the vent valve 11 in the middle part of opening in the bypass line 13 of feed pipe front-end and back-end.
Steam regulation diaphragm valve, control pressure, monitors pressure by tensimeter 12, and pressure-controlling is in 0.15MPa left and right, approximately 20 minutes time.
Reach 121 while spending in temperature, drain valve 9 is closed, and the steam diaphragm valve on steam-pipe 2 is closed, and stops passing into steam.When equitemperature is spent lower than 121, drain valve 9 is opened automatically again, opens the steam diaphragm valve on steam-pipe 2, continues to pass into steam.
After sterilizing time of arrival, close three vent valves 11 on branch's pipeline 13, then steam off diaphragm valves.Open raw material material inlet valve 6 and filter material material inlet valve 7, start product pump between dosing, by the liquid from preparing between dosing from feed pipe 1 along raw material material inlet valve 6, liquid filter 4, filter material material inlet valve 7 pass into bio-reactor 5.
Claims (4)
1. the germ-free charging device of a zooblast suspension culture bio-reactor, comprise feed pipe (1), steam-pipe (2), gas exhaust duct (3), liquid filter (4), bio-reactor (5), it is characterized in that: described feed pipe (1) is along being provided with in order raw material material inlet valve (6), liquid filter (4), filter material material inlet valve (7), bio-reactor (5) on feedstock direction; Feed pipe (1) is divided into anterior feed pipe, middle part feed pipe and rear-fed pipeline by described raw material material inlet valve (6) and filter material material inlet valve (7); On feed pipe (1) between described raw material material inlet valve (6) and liquid filter (4), be connected with steam-pipe (2); On described steam-pipe (2), be provided with steam control valve (10); The front-end and back-end of described middle part feed pipe are connected with respectively the bypass line (13) that is communicated to gas exhaust duct (3), are respectively arranged with vent valve (11) and drain valve (9) on described branch pipeline (13).
2. the germ-free charging device of zooblast suspension culture bio-reactor according to claim 1, is characterized in that: the rear end of described middle part feed pipe is provided with two bypass line (13).
3. the germ-free charging device of zooblast suspension culture bio-reactor according to claim 1, is characterized in that: in described bypass line (13), be also provided with temperature sensor (8).
4. the germ-free charging device of zooblast suspension culture bio-reactor according to claim 1, is characterized in that: on described liquid filter (4), be provided with tensimeter (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410118596.5A CN103911286A (en) | 2014-03-27 | 2014-03-27 | Sterile charging device of animal cell suspension culture bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410118596.5A CN103911286A (en) | 2014-03-27 | 2014-03-27 | Sterile charging device of animal cell suspension culture bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103911286A true CN103911286A (en) | 2014-07-09 |
Family
ID=51037294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410118596.5A Pending CN103911286A (en) | 2014-03-27 | 2014-03-27 | Sterile charging device of animal cell suspension culture bioreactor |
Country Status (1)
| Country | Link |
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| CN (1) | CN103911286A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223217A (en) * | 1990-01-29 | 1993-06-29 | Capsulit S.P.A. | Process and device for the sterilization of filling plants |
| CN101843909A (en) * | 2010-05-13 | 2010-09-29 | 上海高机生物工程有限公司 | Pollution-free sterilization system for bioreactor |
| CN201729834U (en) * | 2010-04-30 | 2011-02-02 | 上海天士力药业有限公司 | Animal cell culture bioreactor aseptic device and bioreactor comprising same |
| CN202139233U (en) * | 2011-07-29 | 2012-02-08 | 北京清大天一科技有限公司 | Bioreactor steam sterilizing system |
| CN203319996U (en) * | 2013-05-10 | 2013-12-04 | 佛山金葵子植物营养有限公司 | Fermentation tank |
| CN203373336U (en) * | 2013-07-17 | 2014-01-01 | 南京朗恩生物科技有限公司 | Sterile feeding device for fermentation tanks |
-
2014
- 2014-03-27 CN CN201410118596.5A patent/CN103911286A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223217A (en) * | 1990-01-29 | 1993-06-29 | Capsulit S.P.A. | Process and device for the sterilization of filling plants |
| CN201729834U (en) * | 2010-04-30 | 2011-02-02 | 上海天士力药业有限公司 | Animal cell culture bioreactor aseptic device and bioreactor comprising same |
| CN101843909A (en) * | 2010-05-13 | 2010-09-29 | 上海高机生物工程有限公司 | Pollution-free sterilization system for bioreactor |
| CN202139233U (en) * | 2011-07-29 | 2012-02-08 | 北京清大天一科技有限公司 | Bioreactor steam sterilizing system |
| CN203319996U (en) * | 2013-05-10 | 2013-12-04 | 佛山金葵子植物营养有限公司 | Fermentation tank |
| CN203373336U (en) * | 2013-07-17 | 2014-01-01 | 南京朗恩生物科技有限公司 | Sterile feeding device for fermentation tanks |
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Application publication date: 20140709 |