CN204690013U - The reactor of disposable pneumo lift-type plant root tissue culture - Google Patents

Abstract

The utility model relates to a disposable airlift plant root tissue culture reactor, which belongs to the technical field of plant tissue culture. The utility model provides a disposable airlift plant root tissue culture reactor with simple structure, easy installation, easy sterilization and easy harvesting of cultured products. The main body of the reactor is a closed cavity made of medical plastic film. The aeration plate is fixed on the aeration nozzle. The aeration nozzle is hot-melt connected with the bottom of the reactor main body. Nylon cable ties are fastened and fixedly connected, the compressed air inlet pipe communicates with the inner cavity of the reactor main body through the aeration nozzle, and a flat clamp is installed on the compressed air inlet pipe; the outer surface of the side wall of the reactor main body is connected to the pH probe tube, dissolved oxygen One end of the probe tube is fixedly connected with the liquid outlet end of the liquid inlet pipe; the outer surface of the top of the reactor main body is fixedly connected with the inlet end of the air outlet pipe; a blind plate is installed on the liquid inlet pipe; the outlet end of the air outlet pipe is connected with a filter. The utility model is used for plant tissue culture.

Description

Disposable Airlift Plant Root Tissue Culture Reactor

technical field

The utility model relates to a plant tissue culture reactor, which belongs to the technical field of plant tissue culture.

Background technique

After the 1970s, the cultivation of plant cells/tissues and animal organs mostly used airlift bioreactors. At present, the main structures of existing airlift bioreactors are mostly made of stainless steel or glass. The stainless steel reactor has a good cultivation effect, but the one-time investment is large, the steam sterilization process of the reactor takes a long time, consumes a lot of energy, it is not easy to clean, and it is difficult to harvest the culture products; although the glass reactor is easy to observe, it is fragile and unsafe. The pressure resistance effect is poor, and the reactor cultivation scale is small, especially in the initial stage of reactor design, it is not convenient to explore the reactor design process parameters. In addition, the existing airlift bioreactor is equipped with multi-layer guide rings and other components, which has a complex structure, is easy to install, and is difficult to repair and maintain.

Contents of the invention

The purpose of the utility model is to overcome the shortcomings of the existing plant tissue culture reactor, and provide a disposable airlift plant root tissue culture reactor with simple structure, easy installation, easy sterilization, and easy harvesting of culture products.

The technical schemes that the utility model solves the problems mentioned above are respectively:

Disposable airlift plant root tissue culture reactor, including reactor main body, multiple compressed air inlet pipes, multiple aeration nozzles, multiple aeration sheets, pH probe tube, dissolved oxygen probe tube, liquid inlet tube, blind plate, air outlet pipe, a plurality of flat clips and filters, the main body of the reactor is a closed cavity made of medical plastic film, the aeration sheet is fixed on the aeration nozzle, and the aeration nozzle and The bottom of the main body of the reactor is hot-melt connected, and the air outlet end of the compressed air inlet pipe and the aeration nozzle are fastened and fixedly connected by nylon cable ties. Multiple compressed air inlet pipes are arranged in correspondence with a plurality of aeration nozzles, and the compressed air The inlet pipe communicates with the inner cavity of the reactor main body through the aeration nozzle, and a flat clamp is installed on each compressed air inlet pipe; the upper end of the outer surface of the side wall of the reactor main body is respectively connected to the pH probe tube, one end of the dissolved oxygen probe tube and The liquid outlet end of the liquid inlet pipe is fixedly connected; the pH probe pipe, the dissolved oxygen probe pipe and the liquid inlet pipe are all communicated with the inner cavity of the reactor main body; the outer surface of the top of the reactor main body is fixedly connected with the air inlet end of the air outlet pipe; A blind plate is installed on the liquid inlet pipe; the outlet end of the air outlet pipe is connected with the filter.

The beneficial effect of the utility model relative to the prior art is:

1. Easy to sterilize; the disposable airlift plant root tissue culture reactor of the utility model can be sterilized after being irradiated with cobalt 60, and can be quickly put into use.

2. It is easy to harvest the culture products; after the culture is completed, the culture medium is first discharged from the lower compressed air inlet pipe, and the culture products can be taken out after cutting the upper part of the reactor for disposable airlift plant root tissue culture.

3. Save a lot of time for reactor cleaning and cleaning; compared with traditional stainless steel and glass bioreactors, there is no need for cleaning and cleaning of the reactor before use and after harvesting, saving a lot of time.

4. The disposable airlift plant root tissue culture reactor of the utility model has the characteristics of simple structure, easy installation and maintenance-free.

Description of drawings

Fig. 1 is the structural representation of the reactor of disposable air-lift type plant root tissue culture of the utility model;

Fig. 2 is an enlarged view of part A of Fig. 1 .

The names and labels of the components involved in the above figure are:

Reactor main body 1, compressed air inlet pipe 2, aeration nozzle 3, aeration plate 4, pH probe pipe 5, dissolved oxygen probe pipe 6, liquid inlet pipe 7, blind plate 8, air outlet pipe 9, plate clamp 10, filter Device 11.

detailed description

The utility model will be described in further detail below in conjunction with the accompanying drawings: the present embodiment is implemented under the premise of the technical solution of the utility model, and detailed implementation is provided, but the protection scope of the utility model is not limited to the following implementation example.

Specific embodiment one: as shown in Fig. 1 and Fig. 2, the reactor of disposable air-lift type plant root tissue culture includes reactor main body 1, multiple compressed air intake pipes 2, multiple aeration nozzles 3, multiple Aeration sheet 4, pH probe tube 5, dissolved oxygen probe tube 6, liquid inlet tube 7, blind plate 8, air outlet tube 9, multiple flat clamps 10 and filter 11, the reactor main body 1 is made of medical plastic A closed chamber made of film, the aeration sheet 4 is fixed on the aeration nozzle 3, and the aeration nozzle 3 is thermally connected to the bottom of the reactor main body 1, and the gas outlet end of the compressed air inlet pipe 2 is connected to the The aeration nozzle 3 is fastened and fixedly connected by nylon cable ties, and multiple compressed air inlet pipes 2 are arranged in correspondence with the multiple aeration nozzles 3, and the compressed air inlet pipe 2 passes through the aeration nozzle 3 and the inner cavity of the reactor main body 1 In communication, each compressed air inlet pipe 2 is equipped with a flat clamp 10 (for controlling the amount of compressed air entering the reactor main body 1); One end of the oxygen probe tube 6 and the outlet end of the liquid inlet pipe 7 are fixedly connected; the pH probe tube 5, the dissolved oxygen probe tube 6 and the liquid inlet pipe 7 are all communicated with the inner cavity of the reactor main body 1; The surface is fixedly connected with the air inlet end of the air outlet pipe 9; a blind plate 8 is installed on the liquid inlet pipe 7; the air outlet end of the air outlet pipe 9 is connected with a filter 11.

The pH probe tube 5 is composed of a fixed connection between the pH probe and the connecting tube 1, and the function of the pH probe is to monitor the pH value during the cultivation process; the dissolved oxygen probe tube 6 is composed of a fixed connection between the dissolved oxygen probe and the connecting tube 2, and the dissolved oxygen probe is fixedly connected to the connecting tube 2. The function is to monitor the dissolved oxygen value during the cultivation process.

Preferably: the number of the compressed air intake pipes 2 is four. Can meet the needs of plant tissue culture.

Working process: as shown in Figure 1 and Figure 2, the reactor of the disposable air-lift plant root tissue culture of the utility model can be used directly after being sterilized by cobalt 60 irradiation. Enter the reactor main body 1, and use the pH probe to monitor the pH value in the cultivation process, and use the dissolved oxygen probe to monitor the dissolved oxygen value in the cultivation process. After the inoculation is completed, open the plate holder 10, and the compressed air will pass through the compressed air inlet pipe 2, expose The gas nozzle 3 enters the reactor main body 1, passes through the gas outlet pipe 9, and is discharged by the filter 11. After the inoculation is completed, utilize the blind plate 8 installed on the liquid inlet pipe 7 to seal the liquid outlet.

The reactor main body 1 volume of the utility model is 300L.

Claims (2)

1. A disposable airlift plant root tissue culture reactor, comprising a reactor body (1), multiple compressed air inlet pipes (2), multiple aeration nozzles (3), and multiple aeration sheets ( 4), pH probe tube (5), dissolved oxygen probe tube (6), liquid inlet tube (7), blind plate (8), air outlet tube (9), multiple plate clips (10) and filter (11) , characterized in that: the reactor main body (1) is a closed cavity made of medical plastic film, the aeration sheet (4) is fixed on the aeration nozzle (3), and the aeration nozzle ( 3) Hot-melt connection with the bottom of the reactor main body (1), the air outlet end of the compressed air inlet pipe (2) and the aeration nozzle (3) are fastened and fixedly connected by nylon cable ties, and multiple compressed air inlet pipes ( 2) One-to-one correspondence with multiple aeration nozzles (3), the compressed air inlet pipe (2) communicates with the inner cavity of the reactor main body (1) through the aeration nozzle (3), and each compressed air inlet pipe (2 ) is installed with a plate clip (10); the upper end of the side wall outer surface of the reactor main body (1) is respectively connected to the pH probe tube (5), one end of the dissolved oxygen probe tube (6) and the outlet of the liquid inlet tube (7) The liquid end is fixedly connected; the pH probe tube (5), the dissolved oxygen probe tube (6) and the liquid inlet tube (7) are all communicated with the inner cavity of the reactor main body (1); the top outer surface of the reactor main body (1) is connected to the outlet The air inlet end of the air pipe (9) is fixedly connected; the liquid inlet pipe (7) is provided with a blind plate (8); the air outlet end of the air outlet pipe (9) is connected with the filter (11). 2. The disposable airlift plant root tissue culture reactor according to claim 1, characterized in that: the number of compressed air inlet pipes (2) is four.