CN103908686B - Bioreactor bactericidal unit - Google Patents
Bioreactor bactericidal unit Download PDFInfo
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- CN103908686B CN103908686B CN201410118685.XA CN201410118685A CN103908686B CN 103908686 B CN103908686 B CN 103908686B CN 201410118685 A CN201410118685 A CN 201410118685A CN 103908686 B CN103908686 B CN 103908686B
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- gas exhaust
- pipeline
- bioreactor
- exhaust piping
- air inlet
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to biological technical field, relate to cell suspension cultures bioreactor bactericidal unit. It comprises reactor, air inlet pipeline, gas exhaust piping, is provided with feeding pipe, sampling line, top gas exhaust piping, bottom discharge pipeline on described reactor, on each pipeline, is respectively arranged with control valve; The end of described feeding pipe is connected on air inlet pipeline. The end of described sampling line is connected on gas exhaust piping, is provided with drain valve and temperature sensor on described gas exhaust piping. Of the present invention simple in structure, easy to operate, sterilization time is short, can monitor in real time sterilising temp.
Description
Technical field
The invention belongs to biological technical field, relate to cell suspension cultures bioreactor bactericidal unit.
Background technology
The metabolism that bioreactor is organism provides physics and the chemical environment of an optimization, makes the faster and better growth of biological physical efficiency. In the incubation of cell, must be strict with the requirement that environment in bioreactor meets cell proliferation, reduce its environment of extraneous germ contamination as far as possible. So, must carry out sterilizing to the culture medium of bioreactor.
Existing is high-temp steam sterilizing to the sterilization method of bioreactor, passes into and in bioreactor, carries out sterilizing by high-temperature steam. But the pipeline complexity of existing high-temp steam sterilizing device, complex operation, sterilization time are long, sterilising temp can not digitlization and cause sterilization effect not reach requirement.
Summary of the invention
Technical problem to be solved by this invention is that a kind of bioreactor bactericidal unit simple in structure, easy to operate, that sterilization time is short, that can monitor in real time sterilising temp is provided.
Bioreactor bactericidal unit of the present invention, comprises reactor, air inlet pipeline, gas exhaust piping, is provided with feeding pipe, sampling line, top gas exhaust piping, bottom discharge pipeline on described reactor, on each pipeline, is respectively arranged with control valve; The end of described feeding pipe is connected on air inlet pipeline.
The end of described sampling line is connected on gas exhaust piping.
On described gas exhaust piping, be provided with drain valve.
On feeding pipe between control valve and the reactor of described feeding pipe, be also connected with charging branch road, the end of described charging branch road is connected on gas exhaust piping.
On described reactor, be provided with many feeding pipes and many sampling lines, on the feeding pipe between control valve and the reactor of described every feeding pipe, be connected with charging branch road, on described every charging branch road, be provided with control valve; The end of described many feeding pipes is connected respectively on air inlet pipeline, and the end of described many sampling lines and many charging branch roads is connected respectively on gas exhaust piping.
The end of described bottom discharge pipeline is connected on air inlet pipeline, on described bottom discharge pipeline, is provided with steam control valve, on the gas exhaust piping of described top, is provided with exhaust regulating valve.
The top of described reactor is provided with microvesicle generators pipeline, on described microvesicle generators pipeline, is provided with control valve, and the end of described microvesicle generators pipeline is connected on air inlet pipeline.
The porch of described air inlet pipeline is provided with pressure-reducing valve and steam pressure sensor.
On described gas exhaust piping, be also provided with temperature sensor.
Advantage applies of the present invention exists: 1. directly air inlet pipeline and gas exhaust piping are connected on the pipeline that reactor carries, install simple, and can alternate application on many reactors, saved cost. 2. described in, the end of many feeding pipes is connected respectively on air inlet pipeline, and the end of described many sampling lines and many charging branch roads is connected respectively on gas exhaust piping, enters steam all shorter with the time of row's steam, and sterilizing is thorough. 3. described in, on gas exhaust piping, be provided with drain valve, temperature reaches 121 while spending, and drain valve is closed, and when equitemperature is spent lower than 121, then opens drain valve, has saved steam. 4. described in, on each branch road of gas exhaust piping, be provided with temperature sensor, can record the relation curve between temperature and sterilization effect, and then improve sterilizing efficiency.
Brief description of the drawings
Fig. 1 is embodiment of the present invention structural representation.
Detailed description of the invention
As shown in the figure, a kind of bioreactor bactericidal unit, comprise reactor 01, air inlet pipeline 02, gas exhaust piping 03, on described reactor 01, be provided with feeding pipe 011, sampling line 012, top gas exhaust piping 013, bottom discharge pipeline 014, on each pipeline, be respectively arranged with control valve; The porch of described air inlet pipeline 02 is provided with pressure-reducing valve 07 and steam pressure sensor 08, and pressure-reducing valve 07 is adjusted to fixed value 0.15MPa.
On described reactor 01, be provided with many feeding pipes 011 and many sampling lines 012, on feeding pipe 011 between the control valve of described every feeding pipe 011 and reactor 01, be connected with charging branch road 015, on described every charging branch road 015, be provided with control valve; The end of described many feeding pipes 011 is connected respectively on air inlet pipeline 02, and the end of described many sampling lines 012 and many charging branch roads 015 is connected respectively on gas exhaust piping 03.
The end of described bottom discharge pipeline 014 is connected on air inlet pipeline 02, on described bottom discharge pipeline 014, is provided with steam control valve 05, on described top gas exhaust piping 013, is provided with exhaust regulating valve 06.
The top of described reactor 01 is provided with microvesicle generators pipeline 016, on described microvesicle generators pipeline 016, is provided with control valve, and the end of described microvesicle generators pipeline 016 is connected on air inlet pipeline 02.
On each branch road of described gas exhaust piping 03, be provided with drain valve 04 and temperature sensor 09, in the time that delivery temperature is spent lower than 121, drain valve 04 is crack, carries out exhaust; When temperature reaches 121 while spending, drain valve 04 is closed. The aperture of steam regulation control valve 05 and exhaust regulating valve 06, makes the temperature in reactor 01 remain 121 degree.
Claims (8)
1. a bioreactor bactericidal unit, comprises reactor (01), air inlet pipeline (02), gas exhaust piping (03),On described reactor (01), be provided with feeding pipe (011), sampling line (012), top gas exhaust piping (013),Bottom discharge pipeline (014), is respectively arranged with control valve on each pipeline; It is characterized in that: described feeding pipe(011) end is connected on air inlet pipeline (02); Control valve and the reactor of described feeding pipe (011)(01) on the feeding pipe (011) between, be also connected with charging branch road (015), described charging branch road (015)End be connected on gas exhaust piping (03).
2. bioreactor bactericidal unit according to claim 1, is characterized in that: described sampling line(012) end is connected on gas exhaust piping (03).
3. bioreactor bactericidal unit according to claim 1, is characterized in that: described gas exhaust piping (03)On be provided with drain valve (04).
4. according to the bioreactor bactericidal unit one of claim 1-3 Suo Shu, it is characterized in that: described reactionOn device (01), be provided with many feeding pipes (011) and many sampling lines (012), described every feed pipeOn feeding pipe (011) between the control valve on road (011) and reactor (01), be connected with charging branch road (015),On described every charging branch road (015), be provided with control valve; The end of described many feeding pipes (011) respectivelyBe connected to air inlet pipeline (02) upper, the end of described many sampling lines (012) and many charging branch roads (015)End is connected respectively on gas exhaust piping (03).
5. bioreactor bactericidal unit according to claim 1, is characterized in that: described bottom discharge pipeIt is upper that the end on road (014) is connected to air inlet pipeline (02), on described bottom discharge pipeline (014), is provided with steamingSteam control valve (05), is provided with exhaust regulating valve (06) on described top gas exhaust piping (013).
6. bioreactor bactericidal unit according to claim 1, is characterized in that: described reactor (01)Top be provided with microvesicle generators pipeline (016), on described microvesicle generators pipeline (016), be provided with controlValve, the end of described microvesicle generators pipeline (016) is connected on air inlet pipeline (02).
7. bioreactor bactericidal unit according to claim 1, is characterized in that: described air inlet pipeline (02)Porch be provided with pressure-reducing valve (07) and steam pressure sensor (08).
8. bioreactor bactericidal unit according to claim 1, is characterized in that: on described gas exhaust pipingAlso be provided with temperature sensor (09).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410118685.XA CN103908686B (en) | 2014-03-27 | 2014-03-27 | Bioreactor bactericidal unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410118685.XA CN103908686B (en) | 2014-03-27 | 2014-03-27 | Bioreactor bactericidal unit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103908686A CN103908686A (en) | 2014-07-09 |
| CN103908686B true CN103908686B (en) | 2016-05-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410118685.XA Expired - Fee Related CN103908686B (en) | 2014-03-27 | 2014-03-27 | Bioreactor bactericidal unit |
Country Status (1)
| Country | Link |
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| CN (1) | CN103908686B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19860290A1 (en) * | 1998-12-21 | 2000-06-29 | Melagapparate Gmbh & Co Kg | Medical instruments pre-cleaned by fluid or ultrasound within steam pressure vessel minimizing steam cleaning cycles |
| CN101843909A (en) * | 2010-05-13 | 2010-09-29 | 上海高机生物工程有限公司 | Pollution-free sterilization system for bioreactor |
| CN103239739A (en) * | 2013-05-09 | 2013-08-14 | 江苏丰泽生物工程设备制造有限公司 | Bioreactor overheating water circulation sterilization device and bioreactor overheating water circulation method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2080384U (en) * | 1990-12-20 | 1991-07-10 | 吉林市中心医院 | Percutaneous puncture urethra catheterization device |
| CN2803384Y (en) * | 2005-07-11 | 2006-08-09 | 张成全 | Gas combustion steam sterilizer |
-
2014
- 2014-03-27 CN CN201410118685.XA patent/CN103908686B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19860290A1 (en) * | 1998-12-21 | 2000-06-29 | Melagapparate Gmbh & Co Kg | Medical instruments pre-cleaned by fluid or ultrasound within steam pressure vessel minimizing steam cleaning cycles |
| CN101843909A (en) * | 2010-05-13 | 2010-09-29 | 上海高机生物工程有限公司 | Pollution-free sterilization system for bioreactor |
| CN103239739A (en) * | 2013-05-09 | 2013-08-14 | 江苏丰泽生物工程设备制造有限公司 | Bioreactor overheating water circulation sterilization device and bioreactor overheating water circulation method |
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| Publication number | Publication date |
|---|---|
| CN103908686A (en) | 2014-07-09 |
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Granted publication date: 20160525 Termination date: 20180327 |