CN103907700B - A kind of method and EGCG extracting method improving EGCG content in green tea - Google Patents
A kind of method and EGCG extracting method improving EGCG content in green tea Download PDFInfo
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- CN103907700B CN103907700B CN201410136227.9A CN201410136227A CN103907700B CN 103907700 B CN103907700 B CN 103907700B CN 201410136227 A CN201410136227 A CN 201410136227A CN 103907700 B CN103907700 B CN 103907700B
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Abstract
The present invention relates to and a kind ofly improve the method for EGCG content in green tea, green tea powder and the EGCG extracting method of correspondence thereof.Improve a method for EGCG content in green tea, adopt rumbatron to carry out to green tea the method that irradiation auxiliary treatment improves EGCG extraction efficiency.Present invention also offers the extracting method of EGCG in a kind of green tea in addition, extract yield is higher, and extraction effect is high.The beneficial effect of this patent is: this patent adopts rumbatron to dry tea, tea powder radiation treatment, and irradiation process is simply efficient, simple to operate, avoids the residual, effectively cost-saving of objectionable impurities, can implementation and operation on a large scale.Dry tea after given dose irradiation, tea powder catechin total content and EGCG content improve greatly.The total number of bacterial colony on surface then obviously declines, and has good fresh-keeping bacteriostatic effect.The present invention also adopts the extracting method of lixiviate EGCG after EGCG pre-treatment, greatly improves the extraction yield of EGCG.
Description
Technical field
The present invention relates to and a kind ofly improve the method for EGCG content in green tea, green tea powder and the EGCG extracting method of correspondence thereof.
Background technology
Tealeaves is generally acknowledged a kind of healthy beverage, has more distinctive health-care effecies.What wherein play a major role is tea-polyphenol in tealeaves.Tea-polyphenol, as a kind of natural antioxidants, not only has significantly anti-ageing, the superfluous free radical of elimination human body, fat-reducing slimming, reduce blood sugar, blood fat and cholesterol, preventing cardiovascular disease, the pharmacological functions such as inhibition tumor cell, prevention radiation injury, also have preservation and antisepsis beauty and skin care, antianaphylaxis, anti-diarrhea, anti influenza, strengthening immunity, the various health care functions such as tobacco poisoning, so have important application in fields such as food-processing, medicine, daily-use chemical industries.What play a major role in tea-polyphenol is its catechin composition, and the strongest component of effect is catechin EGCG wherein.Tea Polyphenols in Tea is a kind of material that tealeaves active substance kind content is the highest, and catechin content accounts for about 70% of tea-polyphenol, and EGCG account for catechin total amount 50% ?60%.Therefore, if can improve the content of Tea Polyphenols in Tea and catechin, the especially content of EGCG, then will be conducive to the lifting of tea health-care effect.Meanwhile, also greatly can increase tea drink, the extraction yield of tea-polyphenol and catechin in tea deep processing enterprise, reduce industrial cost.As the exploitation of tea comprehensive processing product, the purification processing of tea-polyphenol is an important theme, and wherein EGCG is the most important thing, in the anti-oxidant result of study of tea all at present, is that the most a large amount of pharmacological testings is also for representative with it especially with EGCG.Such as, EGCG can neuroprotective unit in Parkinson, alzheimer disease from damage.EGCG can suppress HIV ?the mitotic cycle of 1 virus, thus virus replication, has certain effect to acquired immune deficiency syndrome (AIDS) and virus.Therefore improve the ratio of EGCG in tea-polyphenol, there is obvious health value, comprise commercial value.
Food irradiation technique comes from the 1950's, is developed so far and goes through half a century.At present, the food irradiation technique of China attains the international advanced level, and main application fields is food fresh keeping and sterilization.The World Health Organization (WHO), Food and Argriculture OrganizationFAO (FAO) and International Atomic Energy Agency (IAEA) combine declaration: the food of below 10kGy dose delivery does not exist toxicological issues, does not need to make toxicology test.By international General Standard for Irradiated Foods, the irradiation source adopted in Food irradiation application aspect mainly contains 2 classes:
60co and rumbatron.Domestic at present mainly
60co, rumbatron master has several family.Due to
60the radioactivity of Co, offal treatment is difficult, and operational risk is large, and domestic supervision is more and more tighter, and on the other hand, the technology of rumbatron is more and more ripe, and cost declines obviously.Compared to traditional
60co, rumbatron has some obvious advantages.Sharpest edges of rumbatron are its operational safetys, and controllability is strong.Generation and the disappearance of electron beam irradiation then can be controlled by power switch completely, simple to operate, do not need source of radiation, free from environmental pollution, to operator's fanout free region, can directly apply to continuous prodution.Therefore Food irradiation development trend is by based on rumbatron.
The irradiation of past tealeaves mainly concentrates in the research of sterilization fresh function and security aspect, there is no the correlative study report for predetermined substance improvement or raising aspect in tealeaves.The content utilizing rumbatron or cobalt-60 radiosterilize to improve tea catechin there is no report.
Summary of the invention
This patent adopts rumbatron to carry out pre-irradiation treatment to green tea (dry tea, tea powder etc.), can increase EGCG content in tealeaves fast.This patent provides that a kind of technique is simple, efficient, the technology of green non-pollution.
Improve a method for EGCG content in green tea, adopt rumbatron to carry out to green tea the method that irradiation auxiliary treatment improves EGCG extraction efficiency.
Preferably, described green tea is dry tea, tea powder, fresh leaf or tea stalk.
Preferably, described green tea is dry tea, dry tea is put into rumbatron and accepts irradiation, and irradiation dose is 2 ~ 10kGy.
Preferred, described irradiation dose is 7.0kGy.
Present invention also offers the extracting method of EGCG in a kind of green tea in addition, extract yield is higher, and extraction effect is high.
In green tea, an extracting method of EGCG, comprises the steps:
1) green tea is put into electron accelerator irradiation equipment and accept irradiation, irradiation dose is 6 ~ 10kGy;
2) with hot water and there is agent solvent method to carry out lixiviate to the green tea after irradiation, catechin vat liquor is obtained.
Preferably, described step 1) irradiation dose be 7.0kGy.
Preferably, described green tea refers to dry tea, dry tea powder, steams blue or green tea, the blue or green tea powder of steaming, smears tea, matcha powder or tea stalk.
Preferably, described step 2) leach extraction method for 50% ethanolic soln or hot water extraction.
Preferably, described step 2) be specially: with pulverizer, green tea powder is broken into powder, takes powder 0.15g, with the ethanolic soln lixiviate of 25ml50%, lixiviate 30min in the water-bath of 70 DEG C, rocks evenly every 10min, draw the centrifugal 10min of 2ml vat liquor, obtain catechin vat liquor.
The beneficial effect of this patent is: this patent adopts rumbatron to dry tea, tea powder radiation treatment, and irradiation process is simply efficient, simple to operate, avoids the residual, effectively cost-saving of objectionable impurities, can implementation and operation on a large scale.Dry tea after given dose irradiation, tea powder catechin total content reach 329mg/g and 376mg/g, and EGCG content is respectively 180mg/g and 166mg/g.Wherein the EGCG content of dry tea comparatively control group add about 29%, tea powder EGCG comparatively control group adds about 25%.Meanwhile, the content of tea powder and dry tea Free Amino Acids also comparatively control group add about 50%; The total number of bacterial colony on surface then obviously declines, and has good fresh-keeping bacteriostatic effect.
In addition, the present invention also adopts the extracting method of lixiviate EGCG after EGCG pre-treatment, greatly improves the extraction yield of EGCG.
Embodiment
This patent green tea used is roasted green tea; Tea powder is matcha powder; Experiment reagent is market and buys.
Embodiment 1 ?5:
1. irradiation: the irradiation dose of dry tea (popular roasted green tea) be 2 ?10Kgy (concrete dosage is in table 1).
2.EGCG assay
2.1) pulverize: with pulverizer, dry tea is pulverized, so that lixiviate.
2.2) lixiviate: take dry tea powder 0.15g, with the ethanolic soln lixiviate of 25ml50%, in the water-bath of 70 DEG C, lixiviate 30min, rocks evenly every 10min.Draw 2ml vat liquor at centrifugal 10min, obtain catechin liquid to be measured.
2.3) draw 1ml catechin liquid to be measured, be injected in liquid phase bottle, measure the catechin monomers content in dry tea by HPLC method.Experimental result and standard substance are compared, determines the content of 8 kinds of catechins in dry tea.
2.4) according to above step, measure dry tea the highest 329mg/g of catechin content when the dosage of embodiment 4 (7Kgy), comparatively control group adds 33.41%; The content of EGCG also reaches maximum value 180.53mg/g, and comparatively control group adds 29.31%.Test-results illustrates, electron accelerator irradiation more can promote the increase of the dry tea catechin of green tea and EGCG.
Catechin in Tea monomer increasing amount (%) in the different irradiation situation of table 1
Note: all data are all for CK calculates with the tealeaves sample of not radiation treatment.
Embodiment 2:
1. irradiation: the irradiation dose of matcha powder be 2 ?18Kgy (concrete dosage is in table 2).
2.EGCG assay
2.1. lixiviate: take matcha powder 0.15g, with the ethanolic soln lixiviate of 25ml50%, in the water-bath of 70 DEG C, lixiviate 30min, rocks evenly every 10min.Draw 2ml vat liquor at centrifugal 10min, obtain catechin liquid to be measured.
2.2. draw 1ml catechin liquid to be measured, be injected in liquid phase bottle, measure the catechin monomers content in matcha powder by HPLC method.Experimental result and standard substance are compared, determines the content of 8 kinds of catechins in matcha powder.
2.3. according to above step, measure matcha powder the highest 376.5mg/g of catechin content when embodiment 7, comparatively control group adds 25.3%; The content of EGCG also reaches maximum value 166.5mg/g, and comparatively control group adds 25.%.Test-results illustrates, electron accelerator irradiation more can promote the increase of matcha powder catechin and EGCG.
Catechin monomers increasing amount (%) in tea powder in the different irradiation situation of table 2
Note: all data are all for CK calculates with the tealeaves sample of not radiation treatment.
Claims (6)
1. improve a method for EGCG content in green tea, it is characterized in that: adopt rumbatron to carry out irradiation to green tea, described green tea is dry tea, dry tea is put into rumbatron and accepts irradiation, and irradiation dose is 7.0 ~ 10kGy.
2. the method for EGCG content in raising green tea according to claim 1, is characterized in that: described irradiation dose is 7.0kGy.
3. the method for EGCG content in raising green tea according to claim 1, is characterized in that: described green tea is tea powder, fresh leaf or tea stalk.
4. an extracting method of EGCG in green tea, comprises the steps:
1) green tea is put into electron accelerator irradiation equipment and accept irradiation, irradiation dose is 7.0 ~ 10kGy;
2) with hot water or there is agent solvent method to carry out lixiviate to the green tea after irradiation, catechin vat liquor is obtained.
5. the extracting method of EGCG in green tea according to claim 4, is characterized in that: described step 1) irradiation dose be 7.0kGy.
6. the extracting method of EGCG in the green tea according to claim 4 or 5, is characterized in that: described step 2) leach extraction method for ethanolic soln or hot water extraction.
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| CN101381359A (en) * | 2008-10-15 | 2009-03-11 | 山东农业大学 | Method for extracting high-purity epigallocatechin-3-gallate from green tea |
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| CN101381359A (en) * | 2008-10-15 | 2009-03-11 | 山东农业大学 | Method for extracting high-purity epigallocatechin-3-gallate from green tea |
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| 不同辐照处理对茶叶品质的影响;沈伟桥等;《茶叶科学》;20020131;第22卷(第1期);第90页左栏第2段、第1.1节和1.2节，第91页右栏第2段和表2 * |
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