CN103893022A - Application of antibacterial peptide as preservative in preparing cosmetics - Google Patents
Application of antibacterial peptide as preservative in preparing cosmetics Download PDFInfo
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- CN103893022A CN103893022A CN201410136983.1A CN201410136983A CN103893022A CN 103893022 A CN103893022 A CN 103893022A CN 201410136983 A CN201410136983 A CN 201410136983A CN 103893022 A CN103893022 A CN 103893022A
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Abstract
The invention provides an application of an antibacterial peptide as a preservative in preparing cosmetics. The antibacterial peptide provided by the invention has the beneficial effects that 1) the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity and can effectively inhibit Gram negative bacteria, Gram positive bacteria, saccharomycetes and moulds; 2) the antibacterial peptide has good compatibility with raw materials of almost cosmetics, and the bacteriostatic ability is not affected by surfactants, proteins and Chinese herbal medicine additives in the cosmetics; and 3) the product does not contain a methanol releaser, highly irritant organic halogen components or chemical synthetic components, so that the product is high in safety and low in irritation.
Description
Technical field
The present invention relates to cosmetic field, be specifically related to antibacterial peptide as antiseptic in the application of preparing in cosmetics.
Background technology
Cosmetics refer to smear, spray or other similar approach, intersperse among any position of human body surface, as skin, hair, refer to toenail, lips and teeth etc., to reach clean, maintain, improve looks, modify and to change outward appearance, or correction human scent, maintains a good state as chemical industrial product or the fine chemical product of object.The use of cosmetics has history very remote.According to investigation, the mankind have brought into use cosmetics from the Old Stone Age, and this period is approximately from before 50,000 years.Over nearly 30 years, rapidly, the mankind make to apply some make up more and more general the development of countries in the world cosmetic industry, and cosmetics develop into mankind's flavouring from luxury goods and add beautiful daily necessities.Science and technology given cosmetics whitening antioxygen, moisturizing moisturizing, pox-dispelling function, crease-resistant, the light speckle of Firm dispel scar, clean makeup removing, control oily toxin expelling, responsive skin, whitening and moisturizing, pore refining, looks improving and the skin nourishing, anti-ly lose one's looks, effect of eliminating toxin and beautifying the skin, moisturizing isolation, thereby enjoy liking of the vast personage of liking to be beautiful.Along with the nutritional labeling that contains in can the progressive cosmetics of science and technology also gets more and more, as: protein, aminoacid, marine polysaccharide, Margarita powder, embryo extract, vitamin, even trace element and rare mineral matter etc., can provide the required nutrient of growing to most of microbe, once the microorganism in environment enters, can breed rapidly, destroy the organoleptic quality of product, the health of infringement consumer.
Antiseptic refers to natural or synthetic chemical composition, for adding food, medicine, pigment, biological sample etc., the corruption causing to postpone growth of microorganism or chemical change.What most cosmetics company was used is all chemosynthesis antiseptic, and chemosynthesis antiseptic all exists the defect that zest is large, toxicity is large, and safety aspect is not very satisfactory.Now consumer is to cosmetics safety special concern, tend to use natural, non-stimulated, without the product of the problems such as skin allergy.In recent years, along with the continuous understanding of people to antiseptic inhibition microbial mechanism, in addition the safety issue that chemosynthesis antiseptic exists, from natural product, find Substance safely and efficiently and become the focus that cosmetics are studied, there is good development prospect, become the new development direction of cosmetics preservative research.
Antibacterial peptide is that the one producing through induction in organism has bioactive micromolecule polypeptide, and molecular weight, in 2000～7000 left and right, is made up of 20～60 amino acid residues.This class active polypeptide majority has the features such as strong basicity, heat stability and broad-spectrum antiseptic.The antibacterial peptide using in the present invention is antibacterial peptide.It can kill wide spectrum pathogenic microorganism fast, and as a kind of active substance of body itself, relatively do not there is immunogenicity, to its have repellence antibacterial less, pathogen is also difficult for it to produce drug resistance, therefore can substitute the antibacterial action of former chemosynthesis antiseptic performance broad-spectrum high efficacy.
Summary of the invention
In order to solve above technical problem, the object of the invention is in conjunction with conventional art and biotechnology, by traditional cosmetics and genetic engineered product configuration combination, the antibacterial peptide that adopts biotechnology to obtain, produce do not add traditional chemical synthetic preservative and can preserve for a long time cosmetics.
Antibacterial peptide as antiseptic in the application of preparing in cosmetics.
Preferably, described antibacterial peptide adopts one or both in Defensins and cecropin.
Preferably, described antibacterial peptide adopts aqueous solution or lyophilized powder.
Preferably, applicable cosmetics scope is liquid, emulsion, cream kind, powder class or bulk.
Preferably, the use amount of described antibacterial peptide in cosmetics is the 0.001%-10% of this cosmetics gross weight.
Preferably, the use pH scope of described antibacterial peptide is 2-8.
The present invention can replace traditional preservatives and apply in cosmetics, and another feature of antiseptic of the present invention is still efficiently antibacterial in pH value neutral environment, and effect of other most of antiseptic substitutes is not very desirable in this environment.
Preferably, alexin adopts α alexin, beta-defensin, one or more in θ alexin.
Preferably, when described antibacterial peptide is below 60 DEG C, add in cosmetics.
Above-mentioned antibacterial principle is as follows: antibacterial peptide is to very strong the having a liking for film, adsorb, merge, change its permeability, cause its content leak and cause its death with membranous structure of pathogenic microorganism tool; Tool broad-spectrum antiseptic, antiviral activity, pathogenic microorganism is difficult for it to produce drug resistance; Antibacterial peptide in the time of working concentration to host cell avirulence.
Antibacterial peptide molecule has the film of having a liking for of height for the cell of various pathogenic microorganisms or the membranous structure of virus.But their mechanism of action is also different in the time of the different pathogenic microorganism of kill bacteria, virus, fungus etc. respectively.
In the time of kill bacteria, it is generally acknowledged that antibacterial peptide/alexin is to play a role by the interaction between positive and negative electric charge.They can be attached to perforation on bacterial cell plasma membrane and form ion duct, cause bacterial cell membrane structural deterioration, cause in born of the same parents that water-soluble substances oozes out in a large number, and finally cause antibacterial death.
And in the time of antifungal, some antibacterial peptide/alexin is the characteristic that can combine closely with chitin by them, destroy structural material-chitin in fungal cell wall and killing fungus.Due to antibacterial peptide antibacterial, the antiviral mechanism of action uniquely, make pathogenic microorganism be difficult to it to produce drug resistance.
The present invention has following beneficial effect:
1) the present invention has broad spectrum antibiotic activity, can effectively suppress gram negative bacteria, positive bacteria, yeast and mycete.
2) with good to most of cosmetic material compatibilities, its bacteriostasis is not subject to the impact of surfactant in cosmetics, protein and Chinese herbal medicine additive.
3) this product does not contain methanol releaser, high irritating organic halogen composition and chemosynthesis composition, and safe, zest is low.
Detailed description of the invention
Below preferably embodiment of the present invention is described in further detail:
Embodiment 1
In embodiment 1, antiseptic is in the application of preparing in essence, specific as follows:
The formula of essence is as shown in table 1 below:
Table 1 essence
| Separate | Sequence number | Standard Chinese title | Raw materials quality content (%) | Composite percentage ratio (%) | Application target |
| A | 1 | Deionized water | 92.66 | 100 | Solvent |
| A | 2 | Glycerol | 5 | 100 | Wetting agent |
| B | 3 | Mel extract | 2 | 100 | Wetting agent |
| A | 4 | EDETATE SODIUM | 0.15 | 100 | Chela mediating recipe |
| A | 5 | Hyaluronate sodium | 0.1 | 100 | Wetting agent |
| C | 6 | Essence | 0.01 | 100 | Fragrant agent |
| C | 7 | Cremophor RH40 | 0.03 | 100 | Solubilizing agent |
| c | 8 | Antibacterial peptide | 0.05 | 100 | Antiseptic |
Operating procedure:
1. A being heated with stirring to mutually to 82-85 DEG C dissolves completely.
2. insulated and stirred 30 minutes.
3. cooling
4. by even C phase dispersed with stirring.
5. in the time of greenhouse cooling to 40 DEG C, add B, C phase, dispersed with stirring is even.
6. in the time that temperature is down to 35 DEG C, discharging
Comparative example 1
Different from the composition of the essence in embodiment 1, deionized water raw materials quality content is 92.71%, and not containing antibacterial peptide.
Embodiment 2
In embodiment 2, antiseptic is in the application of preparing in emulsification system, specific as follows:
The formula of moisturiser is as following table 2:
Table 2 moisturiser
Comparative example 2
Different from the composition of the moisturiser in embodiment 2, deionized water raw materials quality content is 72.45%, and not containing antibacterial peptide.
The operating procedure of embodiment 2 and comparative example 2:
A, B are heated with stirring to respectively to 82-85 DEG C mutually to be dissolved completely.
By A be added to B mutually in, high speed homogenization emulsifying 4 minutes.
Insulated and stirred 30 minutes.
Cooling
In the time of greenhouse cooling to 40 DEG C, add C phase, dispersed with stirring is even.
But when temperature is down to 35 DEG C, discharging.
Embodiment 3: minimal inhibitory concentration test (MIC)
1, inoculation
Be inoculated in respectively culture medium slant for examination bacterium, 37 DEG C, antibacterial activation 18-24h, activate 48-72h at 28 DEG C, mycete.
2, the preparation of bacteria suspension
Get a certain amount of supplying of having activated and try bacterium in the fluid medium conical flask of sterilizing is housed, respectively at cultivating in 37 DEG C, 28 DEG C constant-temperature shaking incubators after 24h, 48-72h as original bacteria liquid, respectively with sterile saline by each original bacteria liquid bacteria suspension that to make containing bacterium number be 106-108CFU/mL.
3, minimal inhibitory concentration value (MIC) is measured
This experiment, taking staphylococcus aureus as indicator bacteria, adopts times dilution methods such as test tube antibacterial peptide antiseptic sample to be carried out to the mensuration of MIC value.First in every sterilizing test tubes, add 1mL physiological saline solution, the antibacterial peptide sample solution 1mL(25mg/mL adding in test tube 1), after mixing, therefrom take out 1mL and add test tube 2, the like, in last test tube, discard 1mL.So far, stock solution is prepared into the diluent of a series of variable concentrations.In every test tube, suck 19mL culture medium again, fully mix, pour into while hot in sterile petri dish, after culture medium solidifying, draw 100 μ L bacteria suspensions, be seeded in culture medium, 37 DEG C, antibacterial is cultivated 18-24h, at 28 DEG C, mycete, cultivates 48-72h.The least concentration of growing using integral asepsis is as the MIC value of test sample solution.
4, experimental result
Experimental result is shown in Table 3.
Table 3
In table, "+" indicates that bacterium colony occurs; "-" represents not have bacterium colony to occur.
As can be seen from Table 3, the cosmetics of preparing containing antibacterial peptide antiseptic are better than the cosmetics antibacterial effect of not preparing containing antibacterial peptide antiseptic, and antibacterial peptide antiseptic to the least concentration of staphylococcus aureus is: 0.012mg/mL.
Embodiment 4: preservation challenge test
The biological preservative that said method is produced carries out microorganism protective system challenge test, and test is with the 12nd cash equivalent Staphylococcus aureus, colibacillus of excrement, pseudomonas aeruginosa, the 8th platinite candidiasis and the 6th generation aspergillus niger as inoculation bacterium.The quality of the interpolation of biological preservative is 10%, inoculated bacteria total concentration 106cfu/ml, and inoculated fungi total concentration 104cfu/ml, observed through 28 days, and data are as shown in following table 4 and 5:
CTFA method test antibacterial peptide antiseptic effect in table 4 embodiment 1
CTFA method test antibacterial peptide antiseptic effect in table 5 embodiment 2
Can be found out by table 4 and 5, this biological preservative, by the test of microorganism protective system challenge, can be used as antiseptic completely and plays antisepsis.Above-mentioned cosmetics preservative is not containing traditional preservatives and antibacterial, and it becomes to be grouped into antibacterial and the multi-functional of the splendid inhibition of fungus by having, and is the system that can support " without adding antiseptic " concept for cosmetics completely.This product has broad spectrum antibiotic activity, can effectively suppress gram negative bacteria, positive bacteria, yeast and mycete.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (8)
- Antibacterial peptide as antiseptic in the application of preparing in cosmetics.
- 2. application as claimed in claim 1, is characterized in that, described antibacterial peptide adopts one or both in Defensins and cecropin.
- 3. application as claimed in claim 1, is characterized in that, described antibacterial peptide adopts aqueous solution or lyophilized powder.
- 4. application as claimed in claim 1, is characterized in that, applicable cosmetics scope is liquid, emulsion, cream kind, powder class or bulk.
- 5. application as claimed in claim 1, is characterized in that, the use amount of described antibacterial peptide in cosmetics is the 0.001%-10% of this cosmetics gross weight.
- 6. application as claimed in claim 1, is characterized in that, the use pH scope of described antibacterial peptide is 2-8.
- 7. application as claimed in claim 2, is characterized in that, described alexin comprises α alexin, beta-defensin, one or more in θ alexin.
- 8. application as claimed in claim 4, is characterized in that, described antibacterial peptide adds in cosmetics below 60 DEG C time.
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| CN201410136983.1A CN103893022A (en) | 2014-04-04 | 2014-04-04 | Application of antibacterial peptide as preservative in preparing cosmetics |
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| CN201410136983.1A CN103893022A (en) | 2014-04-04 | 2014-04-04 | Application of antibacterial peptide as preservative in preparing cosmetics |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106539698A (en) * | 2016-05-23 | 2017-03-29 | 北京微火科技有限公司 | A kind of BB frosts containing Plectasin and preparation method thereof |
| CN107899000A (en) * | 2017-12-05 | 2018-04-13 | 广东药科大学 | The spiral antibacterial peptides of α and surfactant complex and its application |
| CN109692130A (en) * | 2019-01-30 | 2019-04-30 | 威海同丰海洋生物科技有限公司 | A kind of facial mask liquid and preparation method thereof containing alexin |
| CN109692129A (en) * | 2019-01-30 | 2019-04-30 | 威海同丰海洋生物科技有限公司 | A kind of shaving cream and preparation method thereof containing alexin |
| CN112587432A (en) * | 2020-12-25 | 2021-04-02 | 天津强微特生物科技有限公司 | Gel type essential oil containing thiazolidine antibacterial cyclic peptide and preparation method thereof |
| CN113318054A (en) * | 2021-05-10 | 2021-08-31 | 上海高庄生物科技有限公司 | Cosmetic antiseptic composition and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106539698A (en) * | 2016-05-23 | 2017-03-29 | 北京微火科技有限公司 | A kind of BB frosts containing Plectasin and preparation method thereof |
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| CN109692130A (en) * | 2019-01-30 | 2019-04-30 | 威海同丰海洋生物科技有限公司 | A kind of facial mask liquid and preparation method thereof containing alexin |
| CN109692129A (en) * | 2019-01-30 | 2019-04-30 | 威海同丰海洋生物科技有限公司 | A kind of shaving cream and preparation method thereof containing alexin |
| CN112587432A (en) * | 2020-12-25 | 2021-04-02 | 天津强微特生物科技有限公司 | Gel type essential oil containing thiazolidine antibacterial cyclic peptide and preparation method thereof |
| CN113318054A (en) * | 2021-05-10 | 2021-08-31 | 上海高庄生物科技有限公司 | Cosmetic antiseptic composition and preparation method thereof |
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