CN109692130A - A kind of facial mask liquid and preparation method thereof containing alexin - Google Patents
A kind of facial mask liquid and preparation method thereof containing alexin Download PDFInfo
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- CN109692130A CN109692130A CN201910090054.4A CN201910090054A CN109692130A CN 109692130 A CN109692130 A CN 109692130A CN 201910090054 A CN201910090054 A CN 201910090054A CN 109692130 A CN109692130 A CN 109692130A
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- mask liquid
- liposome
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The facial mask liquid and preparation method thereof containing alexin that the invention discloses a kind of, facial mask liquid of the invention includes following components by weight percentage: glycerol 4.0-6.0%, alexin 0.3-0.6%, 1, 3- butanediol 3.0-5.0%, hyaluronic acid 0.1-0.2%, arbutin 1.5-2.5%, polyethylene glycol 1.5-2.5%, carbomer 0.4-0.6%, panthenol 0.4-0.6%, vitamin E liposome 4.0-6.0%, vitamin C liposome 2.0-4.0%, triethanolamine 0.3-0.5%, PEG (60) rilanit special 0.2-0.4%, preservative 0.1-0.5%, essence 0.3-0.6%, surplus is deionized water.Between facial mask liquid each component of the invention have synergistic function, can multiple reparation skin, it is safe and non-stimulating；And preparation method is simple, can industrialized production, be conducive to promote and apply.
Description
Technical field
The present invention relates to daily cosmetics technical fields, and in particular to a kind of to contain the facial mask liquid of alexin and its preparation side
Method.
Background technique
With the development of society and the progress of science and technology, the work and life pressure of people is increasing, problem of environmental pollution
Also increasingly severe.Live in that fast pace, diet be irregular for a long time and the biggish environment of pollution in, cause the skin of people to be resistant to
Ability constantly weakens, and skin sensitivity, impaired situation are increasingly severe, and sub-health state is presented in the skin of more and more people.Cause
This, skin nursing has been increasingly becoming indispensable a part in people's life.
For facial mask as one of beauty product, having improves that skin effects are significant, advantage easy to use and deep consumed
Person's likes.Facial mask using when be to be covered on the non-woven fabrics mask for being soaked with facial mask liquid face 15-20 minutes, in this way can be temporary
When face be isolated with extraneous air and pollution, improve skin temperature, expand skin pore, promote sweat gland secretion and newly old generation
It thanks, increase the oxygen content of skin, be conducive to skin and exclude the product of epidermal cell metabolism and the oil substances of accumulation,
Facial mask liquid in pad pasting penetrates into the cuticula of skin epidermis, and skin is made to become soft, naturally bright and flexible.
Alexin (Defensin) is a kind of micromolecule polypeptide rich in cysteine, usually by 29-54 amino acid group
At including 6-8 Conserved cysteines.Alexin has broad-spectrum anti-microbial activity, can effectively kill viral, thin
Bacterium, fungi, conveyor screw etc. also have cytotoxicity to tumour cell.It yet there are no and be applied to alexin to prepare facial mask
The report of liquid.
Summary of the invention
For the above-mentioned prior art, contain the facial mask liquid of alexin and its preparation side the object of the present invention is to provide a kind of
Method.Facial mask liquid of the invention can quickly repair excessively thin, impaired, the dark yellow skin of epidermis；Can it is effectively antibacterial, take off it is red and swollen, exclude skin
Skin toxin, unimpeded capillary, while epidermal keratinocytes can be softened, Bergamot Mint Extract, pore refining is conducive to blackhead and powder
The removing of thorn；Enhance skin resistance, prevents scar hyperplasia；There is mild sterilization, anti-inflammatory simultaneously, adjust sebum secretion, promote
Epidermal growth enables impaired skin healing not stay pigment, scar；Acid-base scale of skin can also be adjusted, Sage Extract, sterilization disappear
Scorching, pore refining, prevents the generation of wrinkle of skin and dark sore.
To achieve the above object, the present invention adopts the following technical scheme:
The first aspect of the present invention provides application of the alexin in preparation facial mask liquid.
Preferably, the amino acid sequence of the alexin are as follows: CYCRIPACIAGERRYGTCIYGGRLWAFCC；(SEQ ID
NO.1)
It is furthermore preferred that including one or more D- type amino acid in the amino acid sequence of the alexin；Specifically, its
Amino acid sequence are as follows:
CYCRIPACIX1GERRYGX2CIYX3GRLWAX4CC；
Wherein, X1 is Ala or D-Ala；
X2 is Thr or D-Thr；
X3 is Gly or D-Gly；
X4 is Phe or D-Phe；
Wherein at least one of X1, X2, X3 or X4 are D amino acid.
The second aspect of the present invention provides a kind of facial mask liquid containing alexin, by weight percentage includes with the following group
Point:
Glycerol 4.0-6.0%, alexin 0.3-0.6%, 1,3 butylene glycol 3.0-5.0%, hyaluronic acid 0.1-0.2%,
Arbutin 1.5-2.5%, polyethylene glycol 1.5-2.5%, carbomer 0.4-0.6%, panthenol 0.4-0.6%, vitamin E lipid
Body 4.0-6.0%, vitamin C liposome 2.0-4.0%, triethanolamine 0.3-0.5%, PEG (60) rilanit special 0.2-
0.4%, preservative 0.1-0.5%, essence 0.3-0.6%, surplus are deionized water.
Preferably, the preservative is plant extracts preservative；It is furthermore preferred that the plant extracts preservative is celestial being
People slaps extract, is prepared by the following method:
It is beaten after fresh cactus is cleaned, slurries are crushed through vacuum freeze drying to get Radix et Caulis Opuntiae Dillenii extract.
Preferably, the essence is hippophae rhamnoides, is prepared by the following method:
Sea buckthorn fruit is dry, crushing, crosses 40-60 mesh, then uses supercritical CO2Extraction process extracts, extraction
Pressure power 25-30MPa, 35-45 DEG C of extraction temperature, CO2Flow 20-25L/h, entrainer are the ethyl alcohol that volumetric concentration is 95%,
The dosage of entrainer is the 6-8% of raw material weight, and extraction time is 1-2h to get hippophae rhamnoides.
The third aspect of the present invention provides the preparation method of the above-mentioned facial mask liquid containing alexin, comprising the following steps:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) hydrogenated castor
Oil and deionized water are added in agitated kettle, are warming up to 70-80 DEG C, are stirred 10-20 minutes, are obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 30-40 DEG C, be added alexin, hyaluronic acid, arbutin, vitamin E liposome,
Vitamin C liposome, preservative and essence, stir 5-10 minutes, then homogeneous 5-10 minutes to get.
Preferably, in step (1) and step (2), mixing speed is 40-60r/min.
Beneficial effects of the present invention:
(1) present invention prepares facial mask liquid by raw material of alexin for the first time, with height antibiotic property, has pole to drug-fast bacteria
Strong antibacterial, biocidal efficacies；And without toxicity, noresidue, not will lead to bacterial drug resistance.
(2) compatibility between each raw material of facial mask liquid of the invention is good, makes the more uniform exquisiteness of product, improves product pair
The compatibility of skin.
(3) excessive chemical substance is avoided using natural plant extracts as preservative and essence in facial mask liquid of the invention
Addition, ingredient is more natural；And above-mentioned natural plant extracts are while as preservative and essence, moreover it is possible to defence
Collaboration facilitation is generated between element, improves bactericidal effect, moreover it is possible to cell be promoted to grow and accelerate the healing of wound.
(4) between facial mask liquid each component of the invention have synergistic function, can multiple reparation skin, safety it is stingless
Swash；And preparation method is simple, can industrialized production, be conducive to promote and apply.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
As background technology part introduction, alexin has the antimicrobial acivity and significant bactericidal effect of wide spectrum,
In the fields such as genetic engineering of plant for disease resistance and bio-pharmaceuticals extensive application.But the stability of alexin, hydrolytic resistance and
Bioactivity still needs to be further increased, and therefore, carries out structural modification to alexin and has become a hot topic of research.
The introducing of non-natural D- type amino acid is one of the method for alexin structural modification, by appropriately introducing D- type ammonia
Base acid, can be improved stability, hydrolytic resistance and the bioactivity of alexin.But type, the position of the introducing of D- type amino acid
It is the key that influence alexin structural modification effect with quantity, if type, position or the quantity of the D- type amino acid introduced do not conform to
It is suitable, stability, hydrolytic resistance and the bioactivity of alexin can be reduced instead.
In the present invention, the amino acid of used alexin is formed are as follows: CYCRIPACIAGERRYGTCIYGGRLWA FCC；
(SEQ ID NO.1)。
On this basis, the present invention has carried out structural modification to above-mentioned alexin first, by largely testing discovery, presses
Following amino acid composition is modified, and effect is more excellent:
CYCRIPACIX1GERRYGX2CIYX3GRLWAX4CC；
Wherein, X1 is Ala or D-Ala；
X2 is Thr or D-Thr；
X3 is Gly or D-Gly；
X4 is Phe or D-Phe；
Wherein at least one of X1, X2, X3 or X4 are D- type amino acid.
The conventional method in the prior art such as the method that chemical synthesis or genetic engineering can be used for alexin is prepared into
It arrives.
The method of alexin structural modification can be directly incorporated into D- type amino acid in chemical synthesis；Or in genetic engineering
D- type amino acid is added during expression in the medium.
On the basis of carrying out structural modification research to alexin, alexin is also used for the system of facial mask liquid by the present invention for the first time
It is standby.In one embodiment of the present invention, the facial mask liquid is made of the following raw material:
Glycerol 4.0-6.0%, alexin 0.3-0.6%, 1,3 butylene glycol 3.0-5.0%, hyaluronic acid 0.1-0.2%,
Arbutin 1.5-2.5%, polyethylene glycol 1.5-2.5%, carbomer 0.4-0.6%, panthenol 0.4-0.6%, vitamin E lipid
Body 4.0-6.0%, vitamin C liposome 2.0-4.0%, triethanolamine 0.3-0.5%, PEG (60) rilanit special 0.2-
0.4%, preservative 0.1-0.5%, essence 0.3-0.6%, surplus are deionized water.
In view of " preservative " and " essence " its effect using chemistry is relatively simple, and nowadays advocating natural back
Under scape, it is not easy to be approved by consumer containing excessive chemical component in facial mask liquid.Based on this, the present invention is extracted using cactus
Object is used as " preservative ", is used as " essence " using hippophae rhamnoides；Wherein, Radix et Caulis Opuntiae Dillenii extract have significant bacteriostasis and
Antioxidation, hippophae rhamnoides can release a kind of pleasant aromatic odor.Radix et Caulis Opuntiae Dillenii extract and hippophae rhamnoides
While as preservative and essence, moreover it is possible to be generated between alexin and cooperate with facilitation, improve bactericidal effect, moreover it is possible to promote
The healing of wound is grown and accelerated into cell.
Vitamin E has the effects that anti-oxidant, vasodilator and anti-pachylosis；Vitamin C can synthesize collagen and
Mucopolysaccharide reduces damage of the free radical to skin, delays senescence, while can inhibit the deposition and junket ammonia of skin abnormality pigment
The activity of sour enzyme reduces the formation of melanin.But vitamin E and vitamin C are easy to oxidize rotten, if being added with commonsense method
Into mask matrix, it is oxidized easily rotten and ineffective；And vitamin C be it is water-soluble, be not easy the angle that penetrates to the skin
Matter layer, therefore cannot play one's part to the full.
Liposome is a kind of novel bio-carrier, has a sustained release and penetrating equal superperformances, the present invention by vitamin E and
The liposomal encapsulated rear raw material as preparation facial mask liquid of vitamin C, can effectively improve vitamin E and ascorbic utilization
Rate.
For vitamin E liposome and vitamin C liposome, commercially available product can be bought, it can also be by existing side
Method is prepared.Such as:
It is in mass ratio that blank liposome is made in 6:1 by lecithin and cholesterol, with blank liposome and vitamin E/dimension
Raw element C is 9:2 mixing in mass ratio, is ultrasonically treated 3min, and vitamin E or dimension life after stabilized processing and centrifuge separation
Plain C liposome.
In facial mask liquid of the invention, each raw material component is an organic whole, indispensable, has collaboration between raw material
Facilitation.Wherein, there is collaboration to promote to make for alexin, Radix et Caulis Opuntiae Dillenii extract, hippophae rhamnoides and arbutin compatible use
With bactericidal effect can not only be promoted, moreover it is possible to cell be promoted to grow and accelerate the healing of wound；Hyaluronic acid, panthenol, three ethyl alcohol
The intermiscibility of amine and glycerol is good, with the use of product can be improved to the compatibility of skin, while improving the water-retaining property of skin；Dimension
The addition of raw element E liposome and vitamin C liposome, can be improved vitamin E and ascorbic stability, reduce skin-color
Element deposition, dispelling stain and freckle, while there are also the effects of sterilization and anti-inflammatory.
The present invention during the test, successively by raw materials such as " alexins ", " Radix et Caulis Opuntiae Dillenii extract " and " hippophae rhamnoides "
Component is removed, and facial mask liquid is prepared.Then the facial mask liquid of preparation is subjected to bacteriostatic test, as a result, it has been found that, with face of the invention
Film liquid is compared, and the fungistatic effect of facial mask liquid can be reduced by deleting raw material components therein all.
On the basis of facial mask liquid raw material of the invention, it is further added by the raw material of one or more common preparation facial mask liquids,
Such as glycerin monostearate, Quercetin, decanoy acetaldehyde etc., facial mask liquid is prepared, is equally used for bacteriostatic test, as a result sends out
It is existing, compared with facial mask liquid of the invention, on the basis of facial mask liquid raw material of the invention, other raw material components are further added by, to antibacterial
The case where promotion of effect is not significant, or even there are also fungistatic effect reduces occurs.
To sum up, facial mask liquid is prepared by the optimization of selection and dosage to raw material in the present invention.Facial mask liquid of the invention
With multiple efficacies: can quickly repair excessively thin, impaired, the dark yellow skin of epidermis；Can it is effectively antibacterial, take off it is red and swollen, exclude skin poison
Plain, unimpeded capillary, while can soften epidermal keratinocytes, Bergamot Mint Extract, pore refining are conducive to blackhead and acne
It removes；Enhance skin resistance, prevents scar hyperplasia；There is mild sterilization, anti-inflammatory simultaneously, adjust sebum secretion, promote epidermis
Cell growth enables impaired skin healing not stay pigment, scar；Acid-base scale of skin, Sage Extract, bactericidal antiphlogistic, receipts can also be adjusted
Contracting pore prevents the generation of wrinkle of skin and dark sore.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Test material used in the embodiment of the present invention and comparative example is the test material of this field routine, can be passed through
Commercial channel is commercially available.
Embodiment 1: the preparation of facial mask liquid
1. raw material forms (weight percent):
Glycerol 5.0%, alexin 0.5%, 1,3 butylene glycol 4.0%, hyaluronic acid 0.1%, arbutin 2.0%, poly- second
Glycol 1,500 2.0%, carbomer 0.5%, panthenol 0.5%, vitamin E liposome 5.0%, vitamin C liposome 3.0%,
Triethanolamine 0.4%, PEG (60) rilanit special 0.3%, preservative 0.3%, essence 0.4%, surplus is deionized water.
Wherein, the amino acid sequence of the alexin are as follows:
CYCRIPACIX1GERRYGX2CIYX3GRLWAX4CC；
Wherein, X1 D-Ala；X2 is D-Thr；X3 is D-Gly；X4 is D-Phe.
Alexin is to introduce D- type amino in corresponding position according to the replacement site of design using Fmoc solid phase synthesis process
Acid, then by Series extension peptide chain to obtain the final product.
The preservative is Radix et Caulis Opuntiae Dillenii extract, is prepared by the following method:
It is beaten after fresh cactus is cleaned, slurries are crushed through vacuum freeze drying to get Radix et Caulis Opuntiae Dillenii extract.
The essence is hippophae rhamnoides, is prepared by the following method:
Sea buckthorn fruit is dry, crushing, crosses 40 meshes, then uses supercritical CO2Extraction process extracts, extraction pressure
Power 28MPa, 40 DEG C of extraction temperature, CO2Flow 25L/h, entrainer are the ethyl alcohol that volumetric concentration is 95%, and the dosage of entrainer is
The 7% of raw material weight, extraction time are 2h to get hippophae rhamnoides.
2. preparation method:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) hydrogenated castor
Oil and deionized water are added in agitated kettle, are warming up to 75 DEG C, are stirred 15 minutes, are obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 35 DEG C, alexin, hyaluronic acid, arbutin, vitamin E liposome, dimension is added
Raw element C liposome, preservative and essence, stirring 10 minutes, then homogeneous 5 minutes to get.
Embodiment 2: the preparation of facial mask liquid
1. raw material forms (weight percent):
Glycerol 4.0%, alexin 0.6%, 1,3 butylene glycol 3.0%, hyaluronic acid 0.2%, arbutin 1.5%, poly- second
Glycol 1,500 2.5%, carbomer 0.4%, panthenol 0.6%, vitamin E liposome 4.0%, vitamin C liposome 4.0%,
Triethanolamine 0.3%, PEG (60) rilanit special 0.4%, preservative 0.1%, essence 0.6%, surplus is deionized water.
Wherein, the amino acid sequence of the alexin are as follows:
CYCRIPACIX1GERRYGX2CIYX3GRLWAX4CC；
Wherein, X1 Ala；X2 is D-Thr；X3 is Gly；X4 is D-Phe.
Alexin is to introduce D- type amino in corresponding position according to the replacement site of design using Fmoc solid phase synthesis process
Acid, then by Series extension peptide chain to obtain the final product.
The preservative is Radix et Caulis Opuntiae Dillenii extract, and the preparation method is the same as that of Example 1.
The essence is hippophae rhamnoides, and the preparation method is the same as that of Example 1.
2. preparation method:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) hydrogenated castor
Oil and deionized water are added in agitated kettle, are warming up to 70 DEG C, are stirred 20 minutes, are obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 30 DEG C, alexin, hyaluronic acid, arbutin, vitamin E liposome, dimension is added
Raw element C liposome, preservative and essence, stirring 5 minutes, then homogeneous 10 minutes to get.
Embodiment 3: the preparation of facial mask liquid
1. raw material forms (weight percent):
Glycerol 6.0%, alexin 0.3%, 1,3 butylene glycol 5.0%, hyaluronic acid 0.1%, arbutin 2.5%, poly- second
Glycol 1,500 1.5%, carbomer 0.6%, panthenol 0.4%, vitamin E liposome 6.0%, vitamin C liposome 2.0%,
Triethanolamine 0.5%, PEG (60) rilanit special 0.2%, preservative 0.5%, essence 0.3%, surplus is deionized water.
Wherein, the amino acid sequence of the alexin are as follows:
CYCRIPACIX1GERRYGX2CIYX3GRLWAX4CC；
Wherein, X1 Ala；X2 is D-Thr；X3 is Gly；X4 is Phe.
Alexin is to introduce D- type amino in corresponding position according to the replacement site of design using Fmoc solid phase synthesis process
Acid, then by Series extension peptide chain to obtain the final product.
The preservative is Radix et Caulis Opuntiae Dillenii extract, and the preparation method is the same as that of Example 1.
The essence is hippophae rhamnoides, and the preparation method is the same as that of Example 1.
2. preparation method:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) hydrogenated castor
Oil and deionized water are added in agitated kettle, are warming up to 80 DEG C, are stirred 10 minutes, are obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 40 DEG C, alexin, hyaluronic acid, arbutin, vitamin E liposome, dimension is added
Raw element C liposome, preservative and essence, stirring 10 minutes, then homogeneous 10 minutes to get.
Comparative example 1: the preparation of facial mask liquid
1. raw material forms (weight percent):
Glycerol 5.0%, 1,3 butylene glycol 4.0%, hyaluronic acid 0.1%, polyethylene glycol 1500 2.0%, carbomer
0.5%, panthenol 0.5%, vitamin E liposome 5.0%, vitamin C liposome 3.0%, triethanolamine 0.4%, PEG (60) hydrogen
Change castor oil 0.3%, preservative 0.3%, essence 0.4%, surplus is deionized water.
Wherein, preservative is imidazolidinyl urea；Essence is hippophae rhamnoides (preparation is with embodiment 1).
2. preparation method:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) hydrogenated castor
Oil and deionized water are added in agitated kettle, are warming up to 75 DEG C, are stirred 15 minutes, are obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 35 DEG C, add hyaluronic acid, vitamin E liposome, vitamin C liposome, anti-corrosion
Agent and essence, stir 10 minutes, then homogeneous 5 minutes to get.
Comparative example 2:
1. raw material forms (weight percent):
Glycerol 5.0%, 1,3 butylene glycol 4.0%, hyaluronic acid 0.1%, polyethylene glycol 1500 2.0%, carbomer
0.5%, panthenol 0.5%, vitamin E liposome 5.0%, vitamin C liposome 3.0%, triethanolamine 0.4%, PEG (60) hydrogen
Change castor oil 0.3%, preservative 0.3%, essence 0.4%, surplus is deionized water.
Wherein, preservative is Radix et Caulis Opuntiae Dillenii extract (preparation is with embodiment 1)；Essence is emulsifying essence.
2. preparation method:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) hydrogenated castor
Oil and deionized water are added in agitated kettle, are warming up to 75 DEG C, are stirred 15 minutes, are obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 35 DEG C, add hyaluronic acid, vitamin E liposome, vitamin C liposome, anti-corrosion
Agent and essence, stir 10 minutes, then homogeneous 5 minutes to get.
Safety testing:
According in " cosmetics safety technical specification " (version in 2015) Micro biological Tests method, toxicology test method and
The facial mask liquid that the human safety method of inspection prepares embodiment 1- embodiment 3 is tested, and the requirement of specification is as a result met,
It is safe to illustrate that facial mask liquid of the invention uses.
Test example 1: bacteriostatic test
1. test material: facial mask liquid prepared by embodiment 1 and comparative example 1- comparative example 2.
2. the preparation for trying bacterium:
Escherichia coli, staphylococcus aureus, Dipel, bacillus subtilis are connect respectively in sterile room
Aspergillus, brewer's yeast and Candida are seeded in bean sprout juice culture solution, into beef extract culture solution in 28-30 DEG C of item by kind
1-3d is cultivated under part.
3. test method:
Diffusion method is punched using agar, sterile petri dish is divided into 4 groups, every group 4, by the beef extract culture solution after sterilizing
It pours into sterile petri dish, every 15~20mL of ware, draws 0.1mL Escherichia coli, golden yellow with Sterile pipette after its solidification
Staphylococcus, Dipel and bacillus subtilis are added on culture dish for examination bacterium, and uniformly, every group of each culture dish adds for coating
Enter different strains.
Sterile petri dish is divided into 4 groups, every group 3, the bean sprout juice culture solution after sterilizing is poured into sterile petri dish, often
15~20mL of ware draws 0.1mL aspergillus, brewer's yeast and Candida with Sterile pipette after its solidification and is added to training for examination bacterium
It supports on ware, uniformly, different strains is added in every group of each culture dish for coating.
After coating, 4 equidistant holes are then made a call on culture medium with the sterilizing stainless steel punch of outer diameter 4mm.
Equivalent is added in the 30 μ L of facial mask liquid prepared with micro sample adding appliance difference extraction embodiment 1, comparative example 1 and comparative example 2, an intermediate hole
Sterile water does control group, marks.Bacterium is placed at 37 DEG C and is cultivated for 24 hours, mould and yeast are placed at 28 DEG C and cultivated
72h.It measures antibacterial circle diameter (bacteriostatic diameter mm meter), test is repeated 5 times, using 5 average values as antibacterial circle diameter.
4. test result:
Test result is as shown in table 1.
Table 1: bacteriostatic test data
As can be seen from Table 1, facial mask liquid prepared by the present invention to Escherichia coli, staphylococcus aureus, Dipel,
Bacillus subtilis, aspergillus, brewer's yeast and Candida all have significant inhibiting effect；And comparative example 1- comparative example 2 is made
In standby facial mask liquid, due to lacking alexin, Radix et Caulis Opuntiae Dillenii extract and hippophae rhamnoides, under significant to the inhibiting effect of above-mentioned bacterium
Drop, illustrates that alexin, Radix et Caulis Opuntiae Dillenii extract and hippophae rhamnoides can cooperate with enhancing bacteriostasis.
Test example 2: Fibroblast Proliferation Assay
1. test specimen: facial mask liquid prepared by embodiment 1 and comparative example 1- comparative example 2.
Respectively by the dry obtained powder of above-mentioned test specimen, the test sample that concentration is 0.5mg/ml then is configured to PBS
Product solution.
2. test method:
The human fibroblasts prepared (being derived from remaining normal skin after performing the operation, the 4th~10 generation) suspension is configured to
Concentration is 2.5 × 104A/ml is inoculated in 96 orifice plates, and respective volume culture medium is not added in inoculating cell in blank control group, to
After cell is adherent, 20 μ l PBS are added in the every hole of negative control group, and 20 μ l test sample solutions are added in the every hole of sample sets, and (PBS matches
System), every group sets 6 multiple holes, set incubator (37 DEG C, 5%CO2) middle incubation 48 hours, then plus people MTT is (public purchased from Sigma
Department, lot number: 08797HJ) solution (PBS preparation) 20 μ l, continue in CO2Incubator (37 DEG C, 5%CO2) in be incubated for 4h.Take out 96
Orifice plate carefully sucks the culture medium in every hole, and DMSO is added, and is uniformly mixed.With the suction at enzyme-linked immunosorbent assay instrument measurement 570nm
Light value, by the opposite proliferation rate for obtaining sample sets compared with negative control group.
In formula: T- sample well Abs；C- negative control hole Abs；C0- blank control wells Abs.
Test result is shown in Table 2.
Table 2: Fibroblast Proliferation Assay result
The main reason for change of dermis is skin aging, fibroblast are the main cells for forming skin corium.
As the fibroblast quantity in the generation skin corium of aging can gradually decrease, skin thickness is gradually thinning.Therefore promote at
Fibrocyte proliferation is the important channel for solving the problems, such as skin aging.As can be seen from Table 2, facial mask liquid of the invention has collaboration
Promote the effect of human fibroblasts proliferation, it was demonstrated that facial mask liquid of the invention has anti-aging function.
Test example 3: wound healing test
1. test material:
Facial mask liquid prepared by embodiment 1 and comparative example 1- comparative example 2, HACAT cell, being purchased from Shanghai Bo Gu biotechnology has
Limit company, article No. BG183.
2. test method:
The HACAT cell of logarithmic growth phase, is inoculated in 6 well culture plates, merges to HACAT cell 100%, in 6 holes
The equidistant horizontal line of back stroke.Using 100uL pipette tips in cell monolayer longitudinal scratch, culture cell wound model is caused.
It is grouped at random after scratch, respectively control group and test group 1-3, the cell maintenance medium processing of control group equivalent, and test group
The facial mask liquid processing that 1-3 uses embodiment 1 and comparative example 1- comparative example 2 to prepare respectively.Observation scratch and drawn horizontal line after having handled
Situation of change near joint, and take pictures respectively in 0,6,12h, the width of scratch is measured under fluorescence microscope.
3. test result:
Test result is shown in Table 3.
Table 3: the scratch width measured under fluorescence microscope
As can be seen from Table 3, facial mask liquid of the invention can effectively promote the migration fusion of HACAT cell, it was demonstrated that this
The facial mask liquid of invention can promote the healing of wound.
Test example 4: melanin is inhibited to form test
1. test specimen:
Facial mask liquid prepared by embodiment 1 and comparative example 1- comparative example 2.
2. test method:
Human skin melanoma cell SK-MEL-1 is placed in the minimum essential medium containing 10% N of embryo's serum, and
In 37 DEG C and 5%CO2Under conditions of cultivate.The cell of culture is applied to the bottom of 75 hole flasks, cell number is 3 × 105/ hole,
It stands overnight, to make cell adherence on wall.After confirmation, each culture medium is become into the new training containing 10ppm test specimen
Support base.Control group uses the culture medium containing DMSO (dimethyl sulfoxide).
In the above described manner, every kind of culture medium is once become into the new culture medium containing test specimen within every 2 days, until culture
Cell is enough full of flask.Then, culture fluid is taken out, is washed with PBS (phosphate buffer), is re-dissolved in the NaOH of 1N
In, the optical density (opticaldensity, OD) under 500 nanometers is measured, 1 calculates the inhibiting rate that melanin is formed as the following formula,
The results are shown in Table 4.
Formula 1: melanin forms inhibiting rate (%)={ 1- (OD of the OD/ control group of each laboratory sample) } × 100.
Table 4: melanin is inhibited to form test result
| Test specimen | Melanin forms inhibiting rate (%) |
| Embodiment 1 | 50.6 |
| Comparative example 1 | 21.8 |
| Comparative example 2 | 14.5 |
As can be seen from Table 4, compared with comparative example 1 and comparative example 2, facial mask liquid of the invention can significantly inhibit melanin
Formation, whitening effect can be played to skin.
Test example 5: the moisture loss test under dry environment is resisted
1. test specimen:
Facial mask liquid prepared by embodiment 1 and comparative example 1- comparative example 2.
2. test method:
For simulated skin state, the glass slide of 25.4 × 76.2mm is selected, 3M adhesive tape is sticked.The sample of about 200mg is weighed,
It is uniformly applied on glass slide, is put into the instrument of constant temperature and humidity.This experiment carries out under room temperature (25 DEG C), using saturation
Ammonium sulfate come control 80% constant humidity environment.Certain time (30,60,90,120,240 minutes) is weighed afterwards；With water
As blank control, the percentage of water loss of sample is calculated, moisturizing rate, the smaller expression moisturizing of percentage of water loss are compared by the size of percentage of water loss
Effect is better.It the results are shown in Table 5.
Percentage of water loss (%)=(M2-M3)/(M2-M1) × 100%；
In formula, M1- glass slide weight (g), total weight (g) before M2- is placed after smearing, total weight after M3- is placed after smearing
(g)。
Table 5: the moisture loss test result under dry environment is resisted
As can be seen from Table 5, facial mask liquid of the invention can resist dry damage in dry environments, have moisturizing skin makeup
Effect.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
SEQUENCE LISTING
<110>Weihai Tong Feng marine organisms Science and Technology Ltd.
<120>a kind of facial mask liquid and preparation method thereof containing alexin
<130> 2019
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 29
<212> PRT
<213>alexin
<400> 1
Cys Tyr Cys Arg Ile Pro Ala Cys Ile Ala Gly Glu Arg Arg Tyr Gly
1 5 10 15
Thr Cys Ile Tyr Gly Gly Arg Leu Trp Ala Phe Cys Cys
20 25
Claims (10)
1. application of the alexin in preparation facial mask liquid.
2. application according to claim 1, which is characterized in that the amino acid sequence of the alexin are as follows:
CYCRIPACIAGERRYGTCIYGGRLWAFCC。
3. application according to claim 2, which is characterized in that include one or more in the amino acid sequence of the alexin
A D- type amino acid.
4. application according to claim 3, which is characterized in that the amino acid sequence of the alexin are as follows:
CYCRIPACIX1GERRYGX2CIYX3GRLWAX4CC；
Wherein, X1 is Ala or D-Ala；
X2 is Thr or D-Thr；
X3 is Gly or D-Gly；
X4 is Phe or D-Phe；
Wherein at least one of X1, X2, X3 or X4 are D- type amino acid.
5. a kind of facial mask liquid containing alexin, which is characterized in that by weight percentage include following components:
Glycerol 4.0-6.0%, alexin 0.3-0.6%, 1,3 butylene glycol 3.0-5.0%, hyaluronic acid 0.1-0.2%, black bearberry
Glycosides 1.5-2.5%, polyethylene glycol 1.5-2.5%, carbomer 0.4-0.6%, panthenol 0.4-0.6%, vitamin E liposome
4.0-6.0%, vitamin C liposome 2.0-4.0%, triethanolamine 0.3-0.5%, PEG (60) rilanit special 0.2-
0.4%, preservative 0.1-0.5%, essence 0.3-0.6%, surplus are deionized water；
The amino acid sequence of the alexin is as described in claim any one of 2-4.
6. facial mask liquid according to claim 5, which is characterized in that the preservative is plant extracts preservative.
7. facial mask liquid according to claim 6, which is characterized in that the plant extracts preservative is cactus extraction
Object is prepared by the following method:
It is beaten after fresh cactus is cleaned, slurries are crushed through vacuum freeze drying to get Radix et Caulis Opuntiae Dillenii extract.
8. facial mask liquid according to claim 5, which is characterized in that the essence is hippophae rhamnoides, is made by the following method
It is standby to form:
Sea buckthorn fruit is dry, crushing, crosses 40-60 mesh, then uses supercritical CO2Extraction process extracts, extracting pressure
25-30MPa, 35-45 DEG C of extraction temperature, CO2Flow 20-25L/h, entrainer are the ethyl alcohol that volumetric concentration is 95%, entrainer
Dosage be raw material weight 6-8%, extraction time be 1-2h to get hippophae rhamnoides.
9. the preparation method of the described in any item facial mask liquids of claim 5-8, which comprises the following steps:
(1) by glycerol, 1,3 butylene glycol, polyethylene glycol, carbomer, panthenol, triethanolamine, PEG (60) rilanit special and
Deionized water is added in agitated kettle, is warming up to 70-80 DEG C, is stirred 10-20 minutes, is obtained mixed liquor A；
(2) when liquid A to be mixed is cooled to 30-40 DEG C, alexin, hyaluronic acid, arbutin, vitamin E liposome, dimension life is added
Plain C liposome, preservative and essence, stir 5-10 minutes, then homogeneous 5-10 minutes to get.
10. according to the method described in claim 9, it is characterized in that, mixing speed is 40- in step (1) and step (2)
60r/min。
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| CN113876624A (en) * | 2021-10-11 | 2022-01-04 | 浙江臻艾控股(集团)有限公司 | Facial mask essence containing hyaluronic acid and preparation method thereof |
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| CN113876624A (en) * | 2021-10-11 | 2022-01-04 | 浙江臻艾控股(集团)有限公司 | Facial mask essence containing hyaluronic acid and preparation method thereof |
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