CN103891454B

CN103891454B - Method for increasing germination rate of soybean seeds

Info

Publication number: CN103891454B
Application number: CN201410112191.0A
Authority: CN
Inventors: 王光亚
Original assignee: ANHUI HUAXIA AGRICULTURAL TECHNOLOGY Co Ltd
Current assignee: ANHUI HUAXIA AGRICULTURAL TECHNOLOGY Co Ltd
Priority date: 2014-03-24
Filing date: 2014-03-24
Publication date: 2015-06-17
Anticipated expiration: 2034-03-24
Also published as: CN103891454A

Abstract

The invention provides a method for increasing the germination rate of soybean seeds. The method comprises following steps of (1) selecting soybean seeds without damaged seed coats and scab, washing for a plurality of times with sterile water, soaking in 75% alcohol being 1-5 DEG C for 0.5-1 minute, filtering and pouring filtrate I out; (2) adding 0.1% HgCl2 being 1-5 DEG C and soaking for 0.5-1 hour, filtering and pouring filtrate II out; (3) adding the sterile water, washing for 3-5 times, then putting into a nutrient solution and soaking for 0.5-1 hour at 25-30 DEG C, filtering and pouring filtrate III out; and (4) putting the soybean seeds treated in step (3) on fine sand or filter paper for germinating, wherein the temperature is controlled to be 25-30 DEG C. By using the method, not only can damage to the soybeans be reduced, but also the germination rate can be increased remarkably.

Description

Improve the method for Germination of Soybean Seed rate

Technical field

The invention belongs to seed processing technology field, be specifically related to a kind of method improving Germination of Soybean Seed rate.

Background technology

Different percentages of seedgermination is different, and determine a lot of because have of seed germination, some seeds can not germinate in resting stage, and what have is dead, and temperature is also the key factor determining seed sprouting.Seed sprouting, except full grown internal condition itself, still needs suitable environmental condition cooperation and just can carry out.Environmental condition mainly comprises moisture, and (moisture is that seed sprouting institute is definitely necessary, there is moisture, ferment could be movable, the nutrient of seed storage could be hydrolyzed generation effect, cell also could expand elongation), (germination temperature of plant seed is generally the scope of 0 ~ 40 DEG C for temperature, but each plant has its germination thermophilic, namely be best suited for the temperature of germination), (seed comes into play just needs oxygen to carry out respiration to air, so it is too many to broadcast spot watering at planting, seed can rot on the contrary, is exactly because the cause of anoxic) and light etc.

The method of existing process seed raising germination rate mainly adopts the method for some physical treatments, and conventional physical method has electromagnetic field and electrostatic processes, ultrasonic processing method, nuclear radiation processing method, magnetized water treatment method, photon radiation and low energy ion to irradiate the processing method etc. of seed.But mainly utilize high voltage electric field process, as: publication number is the patent of invention of the one " apparatus for treating seeds by electrostatic field " of CN1031168A, utilize electrostatic field to carry out process to seed and make crop yield, in the method, process is carried out to seed and needs high pressure facility, its poor stability and cost is higher.

Summary of the invention

The present invention is directed to the environmental condition of actual needs in soybean germination process, provide a kind of method improving Germination of Soybean Seed rate, the method can not only reduce the infringement to soybean, can also significantly improve germination rate.

Technical scheme of the present invention is achieved in that

Improve a method for Germination of Soybean Seed rate, its step is as follows:

1) select seed coat without damaged, without the soya seeds of scab, with aseptic water washing for several times after, with 75% alcohol-pickled 0.5 ~ 1 minute of 1 ~ 5 DEG C, filtrate was outwelled in filtration;

2) 0.1%HgCl of 1 ~ 5 DEG C is added ₂soak 0.5 ~ 1 hour, filter and outwell filtrate;

3) add aseptic water washing 3 ~ 5 times, then put into nutrient solution immersion 0.5 ~ 1 hour, temperature 25 ~ 30 DEG C, filter and outwell filtrate;

4) be placed on fine sand or filter paper by the soya seeds of step 3) process and sprout, temperature controls at 25 ~ 30 DEG C.

Further, the formula of described nutrient solution is: KMnO ₄1 ~ 1.2g/L, KH ₂pO ₄1 ~ 3g/L, NH ₄nO ₃1.0 ~ 1.6g/L, Na ₂moO ₄20 ~ 25mg/L, Na ₂b ₄o ₅(OH) ₄.8H ₂o40 ~ 55mg/L, FeSO ₄.7H ₂o10 ~ 15mg/L, Na ₂eDTA8 ~ 10mg/L.

Further, the formula of described nutrient solution is: KMnO ₄1.1 ~ 1.2g/L, KH ₂pO ₄2 ~ 3g/L, NH ₄nO ₃1.0 ~ 1.6g/L, Na ₂moO ₄20 ~ 25mg/L, Na ₂b ₄o ₅(OH) ₄.8H ₂o45 ~ 55mg/L, FeSO ₄.7H ₂o10 ~ 15mg/L, Na ₂eDTA8 ~ 10mg/L.The sprouting of this nutrient solution prescription phosphorus element content higher promotion soybean.This is because have many important organic phosphorus compounds and inorganic phosphate ion in soybean plant strain, they are not only the constituent of each organ, and it is movable to participate in many important vital metabolic, soya seeds passes through the high nutrient solution process of phosphorus content, can sprouting gene expression inside inducing soybean, and then promote the sprouting of soybean.

Advantage of the present invention is as follows:

This method first by control temperature at 1 ~ 5 DEG C to soya seeds disinfection, solve because sterilization not thoroughly and the soya seeds germination rate that causes of overlong time and the low problem of germination vigor; Further, by the induction process of nutrient solution, the expression of inducing soybean seed germination gene; Put into by soya seeds on filter paper or fine sand medium again and carry out sprouting process, germination rate increases greatly, and loss percentage is very low.

Embodiment

Embodiment 1

Select seed coat without damaged, without the soya seeds 200 of scab, with aseptic water washing for several times after, with 75% alcohol-pickled 1 minute of 1 DEG C, filtrate was outwelled in filtration; Add the 0.1%HgCl of 1 DEG C ₂soak 0.5 hour, filter and outwell filtrate; Add aseptic water washing 3 times, then put into nutrient solution immersion 0.6 hour, temperature 25 DEG C, filter and outwell filtrate; 4) be placed on fine sand by the soya seeds of step 3) process and sprout, temperature controls at 25 DEG C.The germination rate of soya seeds is 98%, and germination vigor is strong.

The formula of above-mentioned nutrient solution is: KMnO ₄1g/L, KH ₂pO ₄1g/L, NH ₄nO ₃1.2g/L, Na ₂moO ₄20mg/L, Na ₂b ₄o ₅(OH) ₄.8H ₂o45mg/L, FeSO ₄.7H ₂o10mg/L, Na ₂eDTA8mg/L.

Embodiment 2

Select seed coat without damaged, without the soya seeds 200 of scab, with aseptic water washing for several times after, with 75% alcohol-pickled 0.5 minute of 3 DEG C, filtrate was outwelled in filtration; Add the 0.1%HgCl of 3 DEG C ₂soak 0.6 hour, filter and outwell filtrate; Add aseptic water washing 4 times, then put into nutrient solution immersion 0.6 hour, temperature 28 DEG C, filter and outwell filtrate; 4) be placed on filter paper by the soya seeds of step 3) process and sprout, temperature controls at 28 DEG C.The germination rate of soya seeds is 98.5%, and germination vigor is strong.

The formula of above-mentioned nutrient solution is: KMnO ₄1.2g/L, KH ₂pO ₄2g/L, NH ₄nO ₃1.2g/L, Na ₂moO ₄25mg/L, Na ₂b ₄o ₅(OH) ₄.8H ₂o40mg/L, FeSO ₄.7H ₂o12mg/L, Na ₂eDTA10mg/L.

Embodiment 3

Select seed coat without damaged, without the soya seeds 200 of scab, with aseptic water washing for several times after, with 75% alcohol-pickled 0.6 minute of 2 DEG C, filtrate was outwelled in filtration; Add the 0.1%HgCl of 4 DEG C ₂soak 0.8 hour, filter and outwell filtrate; Add aseptic water washing 5 times, then put into nutrient solution immersion 1 hour, temperature 28 DEG C, filter and outwell filtrate; 4) be placed on fine sand by the soya seeds of step 3) process and sprout, temperature controls at 25 DEG C.The germination rate of soya seeds is 99.6%, and germination vigor is strong.

The formula of above-mentioned nutrient solution is: KMnO ₄1.2g/L, KH ₂pO ₄3g/L, NH ₄nO ₃1.2g/L, Na ₂moO ₄23mg/L, Na ₂b ₄o ₅(OH) ₄.8H ₂o50mg/L, FeSO ₄.7H ₂o12mg/L, Na ₂eDTA10mg/L.

Embodiment 4

Select seed coat without damaged, without the soya seeds 200 of scab, with aseptic water washing for several times after, with 75% alcohol-pickled 0.6 minute of 5 DEG C, filtrate was outwelled in filtration; Add the 0.1%HgCl of 5 DEG C ₂soak 1 hour, filter and outwell filtrate; Add aseptic water washing 5 times, then put into nutrient solution immersion 1 hour, temperature 30 DEG C, filter and outwell filtrate; 4) be placed on fine sand by the soya seeds of step 3) process and sprout, temperature controls at 25 DEG C.The germination rate of soya seeds is 99%, and germination vigor is strong.

The formula of above-mentioned nutrient solution is: KMnO ₄1.2g/L, KH ₂pO ₄3g/L, NH ₄nO ₃1.2g/L, Na ₂moO ₄23mg/L, Na ₂b ₄o ₅(OH) ₄.8H ₂o55mg/L, FeSO ₄.7H ₂o15mg/L, Na ₂eDTA9mg/L.

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims

1. improve a method for Germination of Soybean Seed rate, it is characterized in that, its step is as follows:

4) by step 3) soya seeds that processed is placed on fine sand or filter paper and sprouts, and temperature controls at 25 ~ 30 DEG C;

The formula of described nutrient solution is: KMnO ₄1 ~ 1.2g/L, KH ₂pO ₄1 ~ 3g/L, NH ₄nO ₃1.0 ~ 1.6g/L, Na ₂moO ₄20 ~ 25mg/L, Na ₂b ₄o ₅(OH) ₄ ^.8H ₂o 40 ~ 55mg/L, FeSO ₄ ^.7H ₂o10 ~ 15mg/L, Na ₂eDTA 8 ~ 10mg/L.

2. the method for raising Germination of Soybean Seed rate according to claim 1, is characterized in that, the formula of described nutrient solution is: KMnO ₄1.1 ~ 1.2g/L, KH ₂pO ₄2 ~ 3g/L, NH ₄nO ₃1.0 ~ 1.6g/L, Na ₂moO ₄20 ~ 25mg/L, Na ₂b ₄o ₅(OH) ₄ ^.8H ₂o 45 ~ 55mg/L, FeSO ₄ ^.7H ₂o 10 ~ 15mg/L, Na ₂eDTA 8 ~ 10mg/L.