CN107027376A

CN107027376A - The method for improving Germination of Soybean Seed rate

Info

Publication number: CN107027376A
Application number: CN201710284031.8A
Authority: CN
Inventors: 张华传
Original assignee: ANHUI HUACHUANG MODERN AGRICULTURAL TECHNOLOGY Co Ltd
Current assignee: ANHUI HUACHUANG MODERN AGRICULTURAL TECHNOLOGY Co Ltd
Priority date: 2017-04-26
Filing date: 2017-04-26
Publication date: 2017-08-11

Abstract

The present invention proposes a kind of method for improving Germination of Soybean Seed rate, comprises the following steps：1) select soya seeds of kind of the skin without damaged, disease-free spot, aseptic water washing for several times after, with 75% alcohol-pickled 2~3 minutes, filtrate was outwelled in filtering；2) 4~8% sodium hypochlorite for adding 1~5 DEG C are soaked 3~6 minutes, and filtrate is outwelled in filtering；3) add aseptic water washing 3~5 times, place into treatment fluid and soak 4~6 hours, filtrate is outwelled in 25~30 DEG C of temperature, filtering；4) by step 3) treated soya seeds are placed on fine sand or filter paper and are sprouted, and temperature control is at 25~30 DEG C；Wherein, the formula for the treatment of fluid is：10~20mg/L Fe₂SO₄·7H₂O, 60~100mg/L K₂HPO₄, 10~30mg/L GA and 0.01~0.5g/L CNTs.This method can not only reduce the infringement to soybean, moreover it is possible to significantly improve germination rate.

Description

The method for improving Germination of Soybean Seed rate

Technical field

The invention belongs to legume technical field, and in particular to a kind of method of raising Germination of Soybean Seed rate.

Background technology

Soybean is one of big crop of China six, originating from China, Chinese soybean germ plasm resource very abundant.China plants from ancient times Soybean is trained, so far existing thousands of years of plantation history.Soybean contains 38%~45% protein, 18%~23% fat.Together When soybean again contain many physiological activators, such as isoflavones, saponin(e, lecithin, cellulose, polysaccharide.According to the United Nations Food and agricultural organization counts, and soybean protein accounts for the 64.78% of the various crop protein in the world, and soybean oil accounts for edible vegetable oil 32.5%.Food and drug administration is assert：Containing more than 2 grams of soybean protein in all consumable products, it can indicate This product plays the role of to mitigate heart attack.

Different percentages of seedgermination is different, determines that the factor that seed is sprouted has a lot, some seeds can not be sent out in rest period Bud, what is had is dead, and temperature is also the key factor for determining germination.Germination is except full grown inherence itself Outside condition, still need appropriate environmental condition and coordinate and could carry out.Environmental condition mainly includes moisture, and (moisture is germination It is absolutely essential, there is moisture, ferment could be movable, and the nutrient of the storage of seeds, which could be hydrolyzed, to be acted, and cell also could Expansion elongation), temperature (the general scope at 0~40 DEG C of the germination temperature of vegetable seeds, but each plant has its germination suitable Temperature, that is, be best suited for germination temperature), air (seed come into play is accomplished by oxygen carry out respiration, so sowing When water too many, seed can rot on the contrary, be exactly the cause because anoxic) and light etc..

The domestic research for the Germination Technique of soya seeds at present is also relatively on the low side, in the industry general just use or soybean The Stratification technique of seed, Main is：In the container of cleaning, load 50~55 DEG C of warm water, water is 5 times of seed volume, Seed is immersed in warm water and is stirred continuously, stops stirring when water temperature drop is to 30 DEG C, then is soaked 8~12 hours, makes seed Moisture is suctioned, then water outlet, drained away the water, bound up with gauze, the piece of sack or towel soaked is wrapped in outside again, puts in basin alms bowl Row vernalization, this method is referred to as hoting water treatment of seeds, and this is germination accelerating method the most frequently used at present, and this method is simple and convenient, but also deposits In problems：Seed sprouting is slow, and bud gesture is weak, it is irregular to emerge, and big seedling phenomenon is serious, such as seed dormancy, speed of emerging Degree is slower, and bud rate is low.

The content of the invention

The present invention proposes a kind of method for improving Germination of Soybean Seed rate, and this method can not only reduce the damage to soybean Evil, moreover it is possible to significantly improve germination rate.

The technical proposal of the invention is realized in this way：

A kind of method for improving Germination of Soybean Seed rate, comprises the following steps：

1) select soya seeds of kind of the skin without damaged, disease-free spot, aseptic water washing for several times after, with 75% alcohol-pickled 2~3 Minute, filtrate is outwelled in filtering；

2) 4~8% sodium hypochlorite for adding 1~5 DEG C are soaked 3~6 minutes, and filtrate is outwelled in filtering；

3) add aseptic water washing 3~5 times, place into treatment fluid and soak 4~6 hours, 25~30 DEG C of temperature, filtering is fallen Fall filtrate；

4) by step 3) treated soya seeds are placed on fine sand or filter paper and are sprouted, and temperature control is 25~30 ℃；

Wherein, the formula for the treatment of fluid is：10~20mg/L Fe₂SO₄·7H₂O, 60~100mg/L K₂HPO₄, 10~ 30mg/L GA and 0.01~0.5g/L CNTs.

Preferably, the formula for the treatment of fluid is：16mg/LFe₂SO₄·7H₂O、82mg/LK₂HPO₄, 18mg/L GA and 0.23g/ L CNTs.

It is to absorb water first during seed sprouting, kind of skin expansion, a softening is made after seed immersion, can pass through more oxygen and plant Skin enters Interior Seed, while carbon dioxide visible through the seed coat is discharged, the physical state of the inside changes；Next to that air, is planted The vital movement of a series of complex of the son conducted in germination process, only seed is constantly breathed, and obtains energy, It can guarantee that being normally carried out for vital movement；It is finally temperature, temperature is too low, and photosynthesis weakens significantly, and respiration is pressed down System, photosynthetic productivity reduction, the decomposition of Interior Seed nutriment and a series of other physiological activities, are required in suitable temperature The lower progress of degree.

Beneficial effects of the present invention：

1st, will be after the processing of 75% alcohol preliminarily pasteurized, then in 1~5 DEG C relatively low of liquor natrii hypochloritis of relative temperature Disinfect, solve because sterilization thoroughly and the soya seeds germination percentage that does not cause of overlong time and germinating energy is low asks Topic.

2nd, gibberellin (GA) can break seed dormancy as a kind of plant modifying agent, accelerate Starch Hydrolysis in endosperm, promote Germination, monovalent cation necessary to potassium is plant part, it is dissolved in water with ionic condition, its major function with Plant metabolism is relevant.According to the influence of mda content in soya seeds germination characteristic and sprout body, in treatment fluid Add 0.01~0.5g/L CNTs and 60~100mg/L K₂HPO₄, can fast and effectively improve soya seeds germination percentage, Germinating energy and the fresh and alive quality of sprout.

Embodiment

Embodiment 1

Select soya seeds 200 of kind of the skin without damaged, disease-free spot, with aseptic water washing for several times after, soaked with 75% alcohol Filtrate is outwelled in bubble 2.5 minutes, filtering；6% sodium hypochlorite for adding 3 DEG C is soaked 5 minutes, and filtrate is outwelled in filtering；Add sterilized water Rinse 3 times, place into treatment fluid and soak 4 hours, filtrate is outwelled in 25 DEG C of temperature, filtering；Treated soya seeds are put again Sprouted on fine sand, temperature control is at 25 DEG C.

The germination rate of soya seeds is 99.2%, and germinating energy is strong.

The formula for the treatment of fluid is：16mg/L Fe₂SO₄·7H₂O、82mg/L K₂HPO₄, 18mg/L GA and 0.23g/L carbon receives Mitron.

Embodiment 2

Select soya seeds 200 of kind of the skin without damaged, disease-free spot, with aseptic water washing for several times after, soaked with 75% alcohol Filtrate is outwelled in bubble 2 minutes, filtering；4% sodium hypochlorite for adding 2 DEG C is soaked 3 minutes, and filtrate is outwelled in filtering；Add sterilized water punching Wash 4 times, place into treatment fluid and soak 4.6 hours, filtrate is outwelled in 28 DEG C of temperature, filtering；Treated soya seeds are put again Sprouted on filter paper, temperature control is at 25 DEG C.

The germination rate of soya seeds is 99.6%, and germinating energy is strong.

Wherein, the formula for the treatment of fluid is：12mg/LFe₂SO₄·7H₂O、98mg/L K₂HPO₄, 13mg/L GA and 0.015g/ L CNTs.

Embodiment 3

Select soya seeds 300 of kind of the skin without damaged, disease-free spot, with aseptic water washing for several times after, soaked with 75% alcohol Filtrate is outwelled in bubble 3 minutes, filtering；8% sodium hypochlorite for adding 5 DEG C is soaked 6 minutes, and filtrate is outwelled in filtering；Add sterilized water punching Wash 5 times, place into treatment fluid and soak 4 hours, filtrate is outwelled in 30 DEG C of temperature, filtering；Treated soya seeds are placed on again Sprouted on filter paper, temperature control is at 30 DEG C.

The germination rate of soya seeds is 98.7%, and germinating energy is strong.

The formula for the treatment of fluid is：10mg/L Fe₂SO₄·7H₂O、60mg/L K₂HPO₄, 10mg/L GA and 0.5g/L carbon receives Mitron.

Embodiment 4

Select soya seeds 300 of kind of the skin without damaged, disease-free spot, with aseptic water washing for several times after, soaked with 75% alcohol Filtrate is outwelled in bubble 2 minutes, filtering；4% sodium hypochlorite for adding 1 DEG C is soaked 6 minutes, and filtrate is outwelled in filtering；Add sterilized water punching Wash 3 times, place into treatment fluid and soak 4 hours, filtrate is outwelled in 28 DEG C of temperature, filtering；Treated soya seeds are placed on again Sprouted on fine sand, temperature control is at 28 DEG C.

The germination rate of soya seeds is 99.5%, and germinating energy is strong.

The formula for the treatment of fluid is：20mg/L Fe₂SO₄·7H₂O、100mg/L K₂HPO₄, 25mg/L GA and 0.01g/L carbon Nanotube.

The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.

Claims

1. a kind of method for improving Germination of Soybean Seed rate, it is characterised in that comprise the following steps：

1) select soya seeds of kind of the skin without damaged, disease-free spot, aseptic water washing for several times after, with 75% alcohol-pickled 2~3 points Filtrate is outwelled in clock, filtering；

3) add aseptic water washing 3~5 times, place into treatment fluid and soak 4~6 hours, filter is outwelled in 25~30 DEG C of temperature, filtering Liquid；

4) by step 3) treated soya seeds are placed on fine sand or filter paper and are sprouted, and temperature control is at 25~30 DEG C；

Wherein, the formula for the treatment of fluid is：10~20mg/L Fe₂SO₄·7H₂O, 60~100mg/L K₂HPO₄, 10~30mg/L GA and 0.01~0.5g/L CNTs.

2. the method according to claim 1 for improving Germination of Soybean Seed rate, it is characterised in that the formula for the treatment of fluid is： 16mg/L Fe₂SO₄·7H₂O、82mg/L K₂HPO₄, 18mg/L GA and 0.23g/L CNTs.