CN108012619A - A kind of method for treating seeds for improving black rice phenolic acid content - Google Patents
A kind of method for treating seeds for improving black rice phenolic acid content Download PDFInfo
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- CN108012619A CN108012619A CN201711347503.6A CN201711347503A CN108012619A CN 108012619 A CN108012619 A CN 108012619A CN 201711347503 A CN201711347503 A CN 201711347503A CN 108012619 A CN108012619 A CN 108012619A
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- black rice
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- seed
- acid content
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention belongs to black rice planting technology field, especially a kind of method for treating seeds for improving black rice phenolic acid content, specific method is as follows:(1)Black rice seed is immersed in silver nitrate aqueous solution and is soaked, filtrate is pulled out, drains to no water droplet and drips, and inserts and freezing processing is carried out in household freezer, is dried to water content as 4% ~ 6%;(2)It is immersed in seed soaking agent and soaks, filtrate is pulled out, carries out magnetization treatment, is dried to water content as 17% ~ 19%;The seed soaking agent, is made of following raw material:Citric acid chelated zinc, glutamic acid, astragalus polyose, giant knotweed polysaccharide, borax, water;Effectively enhancing seed vitality, prevent pest and disease damage, lift germination percentage and percentage establishment of seedling, seedlings are uniform and strong for promotion, promotes the growth of black rice robust plant, and enhancing absorbs the ability of nutrient, promote the conversion capability to nutrient, the phenolic acid content such as protocatechuic acid, vanillic acid, forulic acid and Quercetin of black rice is significantly improved, health value is lifted, promotes health.
Description
Technical field
The invention belongs to black rice planting technology field, especially a kind of seed treatment for improving black rice phenolic acid content
Method.
Background technology
Black rice is edible, medicine is same uses, and belongs to glutinous rice class, since its appearance is in black, and has abundant nutrition, there is " the world
The good reputation of king in rice ";It is well known that the edible value of black rice is very high, in addition to cooking gruel, make nutraceutical, can also make wine,
Also containing abundant phenolic acid in black rice, phenolic acid is widely distributed, in the plant such as veterinary antibiotics, cereal
All contain phenolic acid, phenolic acid there are a variety of physiological activity, such as anti-oxidant, antithrombus formation, cardioprotection and vasodilation
Effect etc., more edible food rich in phenolic acid are very useful to health;Therefore, invention is a kind of improves black rice phenolic acid class thing
The method of matter content can bring breakthrough progress to the development of black rice industry.
The content of the invention
A kind of in view of the above-mentioned problems, the present invention is intended to provide method for treating seeds for improving black rice phenolic acid content.
The present invention is achieved through the following technical solutions:
A kind of method for treating seeds for improving black rice phenolic acid content, specific method are as follows:
(1)Black rice seed is immersed in the silver nitrate aqueous solution that mass fraction is 0.7% ~ 0.9% and soaks 34 ~ 38min, filtrate is dragged for
Go out, drain to no water droplet and drip, insert and freezing processing is carried out in household freezer, be dried to and contain under conditions of being 44 ~ 46 DEG C in temperature
Water is 4% ~ 6%;
(2)Will be through step(1)Black rice seed after processing is immersed in 66 ~ 70min of immersion, filtrate in seed soaking agent and pulls out, carries out magnetic
Change is handled, and it is 17% ~ 19% to be dried to water content under conditions of being 40 ~ 42 DEG C in temperature;
The seed soaking agent, is made of the raw material of following parts by weight:3.1 ~ 3.3 parts of citric acid chelated zinc, glutamic acid 0.5 ~ 0.7
Part, 0.14 ~ 0.18 part of astragalus polyose, 0.22 ~ 0.26 part of giant knotweed polysaccharide, 2.3 ~ 2.7 parts of borax, 570 ~ 590 parts of water.
As the further scheme of invention:Step(1)The freezing processing, under conditions of temperature is -15 ~ -14 DEG C
Cryostat handles 16 ~ 18min, and being cooled to -24 ~ -23 DEG C with the rate of temperature fall of 0.2 ~ 0.3 DEG C/min continues cryostat processing
21 ~ 23min, is warming up to -3 ~ -2 DEG C with the heating rate of 0.8 ~ 0.9 DEG C/min and continues 9 ~ 11min of cryostat processing.
As the further scheme of invention:Step(2)The magnetization treatment, in the bar that magnetic field intensity is 1730 ~ 1750G
160 ~ 180s of magnetization treatment under part, 240 ~ 260s of magnetization treatment under conditions of magnetic field intensity is 320 ~ 340G, in magnetic field intensity
For 350 ~ 370s of magnetization treatment under conditions of 910 ~ 930G.
Beneficial effects of the present invention:A kind of seed treatment side for improving black rice phenolic acid content provided by the invention
Method, effectively strengthens seed vitality, stimulates the activity of seed inclusion, and supplements the nutrients, supplying energy, and sterilization sterilizing, prevents disease
Insect pest, lifts germination percentage and percentage establishment of seedling, and seedlings are uniform and strong for promotion, promotes the growth of black rice robust plant, and enhancing absorbs the ability of nutrient,
Lifted plant cell activity and enzyme activity, promote the conversion capability to nutrient, effectively facilitate the effective nutrition of black rice seed into turn
It is combined to and accumulates, the phenolic acids such as protocatechuic acid, vanillic acid, forulic acid and the Quercetin of black rice can be significantly improved and contained
Amount, lifts health value, promotes health.
Embodiment
Illustrate the present invention with specific embodiment below, but be not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of method for treating seeds for improving black rice phenolic acid content, specific method is as follows:
(1)Black rice seed is immersed in the silver nitrate aqueous solution that mass fraction is 0.7% and soaks 34min, filtrate is pulled out, drains
Dripped to no water droplet, insert and freezing processing is carried out in household freezer, be dried under conditions of being 44 DEG C in temperature water content be 4% ~
6%;
(2)Will be through step(1)Black rice seed after processing, which is immersed in seed soaking agent, soaks 66min, and filtrate is pulled out, carries out at magnetization
Reason, it is 17% ~ 19% to be dried to water content under conditions of being 40 DEG C in temperature;
The seed soaking agent, is made of the raw material of following parts by weight:3.1 parts of citric acid chelated zinc, 0.5 part of glutamic acid, radix astragali are more
0.14 part of sugar, 0.22 part of giant knotweed polysaccharide, 2.3 parts of borax, 570 parts of water.
As the further scheme of invention:Step(1)The freezing processing, constant temperature under conditions of being -15 DEG C in temperature
Freezing processing 16min, with the rate of temperature fall of 0.2 DEG C/min be cooled to -24 DEG C continue cryostats processing 21min, with 0.8 DEG C/
The heating rate of min is warming up to -3 DEG C and continues cryostat processing 9min.
As the further scheme of invention:Step(2)The magnetization treatment, under conditions of magnetic field intensity is 1730G
Magnetization treatment 160s, the magnetization treatment 240s under conditions of magnetic field intensity is 320G, the magnetic under conditions of magnetic field intensity is 910G
Change processing 350s.
Embodiment 2
In the embodiment of the present invention, a kind of method for treating seeds for improving black rice phenolic acid content, specific method is as follows:
(1)Black rice seed is immersed in the silver nitrate aqueous solution that mass fraction is 0.8% and soaks 36min, filtrate is pulled out, drains
Dripped to no water droplet, insert and freezing processing is carried out in household freezer, be dried under conditions of being 45 DEG C in temperature water content be 4% ~
6%;
(2)Will be through step(1)Black rice seed after processing, which is immersed in seed soaking agent, soaks 68min, and filtrate is pulled out, carries out at magnetization
Reason, it is 17% ~ 19% to be dried to water content under conditions of being 41 DEG C in temperature;
The seed soaking agent, is made of the raw material of following parts by weight:3.2 parts of citric acid chelated zinc, 0.6 part of glutamic acid, radix astragali are more
0.16 part of sugar, 0.24 part of giant knotweed polysaccharide, 2.5 parts of borax, 580 parts of water.
As the further scheme of invention:Step(1)The freezing processing, it is permanent under conditions of being -14.5 DEG C in temperature
Warm freezing processing 17min, is cooled to -23.5 DEG C with the rate of temperature fall of 0.25 DEG C/min and continues cryostat processing 22min, with
The heating rate of 0.85 DEG C/min is warming up to -2.5 DEG C and continues cryostat processing 10min.
As the further scheme of invention:Step(2)The magnetization treatment, under conditions of magnetic field intensity is 1740G
Magnetization treatment 170s, the magnetization treatment 250s under conditions of magnetic field intensity is 330G, the magnetic under conditions of magnetic field intensity is 920G
Change processing 360s.
Embodiment 3
In the embodiment of the present invention, a kind of method for treating seeds for improving black rice phenolic acid content, specific method is as follows:
(1)Black rice seed is immersed in the silver nitrate aqueous solution that mass fraction is 0.9% and soaks 38min, filtrate is pulled out, drains
Dripped to no water droplet, insert and freezing processing is carried out in household freezer, be dried under conditions of being 46 DEG C in temperature water content be 4% ~
6%;
(2)Will be through step(1)Black rice seed after processing, which is immersed in seed soaking agent, soaks 70min, and filtrate is pulled out, carries out at magnetization
Reason, it is 17% ~ 19% to be dried to water content under conditions of being 42 DEG C in temperature;
The seed soaking agent, is made of the raw material of following parts by weight:3.3 parts of citric acid chelated zinc, 0.7 part of glutamic acid, radix astragali are more
0.18 part of sugar, 0.26 part of giant knotweed polysaccharide, 2.7 parts of borax, 590 parts of water.
As the further scheme of invention:Step(1)The freezing processing, constant temperature under conditions of being -14 DEG C in temperature
Freezing processing 18min, with the rate of temperature fall of 0.3 DEG C/min be cooled to -23 DEG C continue cryostats processing 23min, with 0.9 DEG C/
The heating rate of min is warming up to -2 DEG C and continues cryostat processing 11min.
As the further scheme of invention:Step(2)The magnetization treatment, under conditions of magnetic field intensity is 1750G
Magnetization treatment 180s, the magnetization treatment 260s under conditions of magnetic field intensity is 340G, in the condition that magnetic field intensity is 910 ~ 930G
Lower magnetization treatment 370s.
Comparative example 1
The normal treatment process of the black rice seed of the prior art
Comparative example 2
A kind of method for treating seeds for improving black rice phenolic acid content, difference lies in by step with embodiment 1(1)Institute
The silver nitrate aqueous solution stated is replaced with water, other conditions all same.
Comparative example 3
A kind of method for treating seeds for improving black rice phenolic acid content, difference lies in step with embodiment 1(1)Not into
Row freezing processing, other conditions all same.
Comparative example 4
A kind of method for treating seeds for improving black rice phenolic acid content, difference lies in by step with embodiment 1(2)Institute
The seed soaking agent stated is replaced with water, other conditions all same.
Comparative example 5
A kind of method for treating seeds for improving black rice phenolic acid content, difference lies in step with embodiment 1(2)Not into
Row magnetization treatment, other conditions all same.
At with a collection of " black No. 3 of ocean " black rice seed using the processing method of embodiment and the processing method of comparative example
Reason, is sowed after processing, is sowed 8 mu, is equally divided into 8 groups, per 1 mu of multicast kind, in addition to method for treating seeds is different, other
Part all same, detects protocatechuic acid content, vanilla acid content, ferulaic acid content and quercetin content after harvesting, as a result such as table 1:
The comparing result of 1 embodiment of table and comparative example
Protocatechuic acid content(mg/kg) | Vanilla acid content(mg/kg) | Ferulaic acid content(mg/kg) | Quercetin content(mg/kg) | |
Embodiment 1 | 98.99 | 166.87 | 386.11 | 123.72 |
Embodiment 2 | 99.32 | 164.77 | 389.98 | 122.56 |
Embodiment 3 | 98.76 | 165.73 | 387.65 | 121.38 |
Comparative example 1 | 27.21 | 43.27 | 167.87 | 34.55 |
Comparative example 2 | 72.64 | 125.46 | 322.43 | 94.58 |
Comparative example 3 | 75.32 | 129.58 | 326.84 | 96.73 |
Comparative example 4 | 64.38 | 104.65 | 287.69 | 76.56 |
Comparative example 5 | 70.21 | 120.08 | 317.68 | 95.44 |
As it can be seen from table 1 the method for treating seeds of the present invention can significantly improve the protocatechuic acid, vanillic acid, asafoetide of black rice
The phenolic acid content such as acid and Quercetin, lifts its health value.
Claims (3)
1. a kind of method for treating seeds for improving black rice phenolic acid content, it is characterised in that specific method is as follows:
(1)Black rice seed is immersed in the silver nitrate aqueous solution that mass fraction is 0.7% ~ 0.9% and soaks 34 ~ 38min, filtrate is dragged for
Go out, drain to no water droplet and drip, insert and freezing processing is carried out in household freezer, be dried to and contain under conditions of being 44 ~ 46 DEG C in temperature
Water is 4% ~ 6%;
(2)Will be through step(1)Black rice seed after processing is immersed in 66 ~ 70min of immersion, filtrate in seed soaking agent and pulls out, carries out magnetic
Change is handled, and it is 17% ~ 19% to be dried to water content under conditions of being 40 ~ 42 DEG C in temperature;
The seed soaking agent, is made of the raw material of following parts by weight:3.1 ~ 3.3 parts of citric acid chelated zinc, glutamic acid 0.5 ~ 0.7
Part, 0.14 ~ 0.18 part of astragalus polyose, 0.22 ~ 0.26 part of giant knotweed polysaccharide, 2.3 ~ 2.7 parts of borax, 570 ~ 590 parts of water.
2. the method for treating seeds according to claim 1 for improving black rice phenolic acid content, it is characterised in that step
(1)The freezing processing, temperature be -15 ~ -14 DEG C under conditions of cryostat handle 16 ~ 18min, with 0.2 ~ 0.3 DEG C/
The rate of temperature fall of min is cooled to -24 ~ -23 DEG C and continues cryostat 21 ~ 23min of processing, with the heating speed of 0.8 ~ 0.9 DEG C/min
Rate is warming up to -3 ~ -2 DEG C and continues 9 ~ 11min of cryostat processing.
3. the method for treating seeds according to claim 1 for improving black rice phenolic acid content, it is characterised in that step
(2)The magnetization treatment, 160 ~ 180s of magnetization treatment under conditions of magnetic field intensity is 1730 ~ 1750G, is in magnetic field intensity
240 ~ 260s of magnetization treatment under conditions of 320 ~ 340G, magnetic field intensity be 910 ~ 930G under conditions of magnetization treatment 350 ~
370s。
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109006297A (en) * | 2018-08-17 | 2018-12-18 | 界首市鑫康家庭农场 | A kind of implantation methods improving green turnip bioactive substance content |
CN110881525A (en) * | 2018-09-11 | 2020-03-17 | 南京农业大学 | Production technology of highland barley milk beverage rich in phenolic acid |
CN110933968A (en) * | 2018-09-21 | 2020-03-31 | 南京农业大学 | Method for increasing content of syringic acid and p-coumaric acid in soybean sprouts |
CN110974911A (en) * | 2019-11-22 | 2020-04-10 | 中国农业科学院烟草研究所 | Method for extracting phenolic acid compounds from wild rice by using deep eutectic solvent |
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2017
- 2017-12-15 CN CN201711347503.6A patent/CN108012619A/en active Pending
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109006297A (en) * | 2018-08-17 | 2018-12-18 | 界首市鑫康家庭农场 | A kind of implantation methods improving green turnip bioactive substance content |
CN110881525A (en) * | 2018-09-11 | 2020-03-17 | 南京农业大学 | Production technology of highland barley milk beverage rich in phenolic acid |
CN110933968A (en) * | 2018-09-21 | 2020-03-31 | 南京农业大学 | Method for increasing content of syringic acid and p-coumaric acid in soybean sprouts |
CN110974911A (en) * | 2019-11-22 | 2020-04-10 | 中国农业科学院烟草研究所 | Method for extracting phenolic acid compounds from wild rice by using deep eutectic solvent |
CN110974911B (en) * | 2019-11-22 | 2021-07-27 | 中国农业科学院烟草研究所 | Method for extracting phenolic acid compounds from wild rice by using deep eutectic solvent |
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Application publication date: 20180511 |