CN103877140A - Application of saussurea involucrate culture in preparation of product for promoting bone health - Google Patents
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- CN103877140A CN103877140A CN201410136941.8A CN201410136941A CN103877140A CN 103877140 A CN103877140 A CN 103877140A CN 201410136941 A CN201410136941 A CN 201410136941A CN 103877140 A CN103877140 A CN 103877140A
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Abstract
The invention discloses application of a saussurea involucrate culture in preparation of a product for promoting bone health. The application comprises preparation of a food or a drug for promoting bone health. The effect of promoting bone health is prevention and treatment of osteoporosis, improvement of the calcium content of the bone, increase of the bone mineral density, improvement of rheumatoid arthritis and treatment of arthritis. An animal test and a clinical test show that the saussurea involucrate culture can be used for improving the calcium content of the bone, and increasing the bone mineral density to prevent and treat osteoporosis, and can be used for improving the rheumatoid arthritis, so as to be applied to the food or drug for promoting bone health.
Description
Technical field
The present invention relates to food and medical technical field, particularly Saussurea involucrata culture promotes the application in bone health product in preparation.
Background technology
Osteoporosis is a kind of low with bone amount, and bone micro-structure damages, and causes bone fragility to increase, and the general osteopathia of fracture for feature easily occurs.This is a kind of degenerative disorders of skeleton, follows age growth, and ill risk increases.Along with the arrival of human longevity prolongation and aging society, osteoporosis has become the whole mankind's important health problem.Osteoporosis is also commonly encountered diseases and the frequently-occurring disease that threatens menopausal women.Osteoporosis is by due to many factors, and its basic pathology mechanism is that in bone metabolism process, defect appears in bone resorption and osteoplastic coupling, causes the alcium and phosphor metabolization imbalance in human body, bone density reduced gradually and the clinical symptoms that causes.The modal disease disease of osteoporosis, common with lumbago and backache, account for 70%～80% in pain patients.Pain spreads to both sides along spinal column, lies on the back or pain relief when seat, stretches after when upright or the pain increased when standing for a long time, sitting, and pain is light in the daytime, and night and early morning increase the weight of while waking up, bends over, muscular movement, cough, stool increase the weight of while exerting oneself.
Bone calcium is the content of calcium constituent in skeleton, and it is the important pathological characters of osteoporosis that calcium content of bone reduces.Bone density full name is skeleton mineral density.The height of bone density is to weigh the how many standard of bone amount, reflects osteoporotic degree, the important evidence of prediction fracture risk.Improve calcium content in skeleton, increase bone density, can prevent and treat osteoporosis.
Rheumatoid arthritis (RA) is a kind of autoimmune disease taking articular synovitis as feature, and pathogenesis is very complicated, illustrates not yet completely so far.Its clinical manifestation be arthralgia, swelling, joint stuffiness, morning deadlock etc.In RA, the persistence hypertrophy of synovial membrane and the destruction of cartilage and bone are its important pathological characteristicses.The animal model of RA has multiple, and the rat arthritis of Freund's complete adjuvant induction is one of comparatively classical model.
Saussurea involucrata culture has no report in the effect aspect raising calcium content of bone, increase bone density, prevention and treatment osteoporosis.
Mono-kind of ZL201210213551.7 treats the Traditional Chinese medicinal liquor of osteoporosis, record " with Herba Saussureae Involueratae, Cacumen Tamaricis, Cortex Illicii, stork grass, Rhizoma Curculiginis spleen reinforcing stomach function regulating; spleen invigorating lung benefiting; bone and muscle strengthening ", also recorded " Herba Saussureae Involueratae is for rheumatic arthritis, arthralgia; lung cold cough; kidney and cold and pain in the lower abdomen, leukorrhagia etc. ", do not recorded Herba Saussureae Involueratae or Saussurea involucrata culture and there are the bone density of increasing, control osteoporosis function and relevant pharmacy data.
201310299562.6 1 kinds of patent applications are treated the Chinese medicine composition of menopausal women osteoporosis, record traditional effect of Herba Saussureae Involueratae, " cure mainly snow the region between the heart and the diaphragm, toothache, rheumatic arthritis, sexual impotence, menoxenia, redly collapse, the disease such as leucorrhea, gastric ulcer, hemorrhoid, bronchitis, heart disease, epistaxis and venom; restore menstrual flow and invigorate blood circulation; dim palace scattered silt, dispelling cold and removing dampness, strong muscle is supporing yang, the function of hemostasis, detumescence ", do not record Herba Saussureae Involueratae or Saussurea involucrata culture increase bone density yet, prevent osteoporotic effect and relevant pharmacy data.
ZL200710017385.2 has the pharmaceutical composition and preparation and the method for quality control that increase bone substance density improving function, recorded " the Chinese medicine Rhizoma Drynariae of selecting has effect of invigorating the kidney and strengthening the bones, bone and muscle strengthening; be aided with the Rhizoma Cimicifugae with anti-osteoporosis activity simultaneously; both synergism improve sclerotin increase bone density " and " select calcium citrate, Margarita powder and calcium carbonate as calcium source; prevention and alleviate bone loss with prevent fracture generation, maintain bone strength; Supplement phosphopeptide caseinate simultaneously and promote that human body has good absorption to calcium ", do not record Herba Saussureae Involueratae or Saussurea involucrata culture and there are the effect and the relevant pharmacy data that increase bone density, improve bone calcium.
Aspect arthritis, patent ZL201010519409.6 has disclosed " Saussurea involucrata culture compositions found through experiments; prevention and releasing arthritis effect are remarkable ", but the Saussurea involucrata culture compositions that this patent provides contains following component: Saussurea involucrata culture, cucumber seed powder, Semen Lactucae sativae powder, celery seed powder and, calcium source, and Saussurea involucrata culture is " making up the unicity that Semen Allii Tuberosi based food carries out arthritis health care on the market " and occur the independent action of not mentioned Saussurea involucrata culture aspect arthritis.In the embodiment of antiphlogistic effects, the animal model adopting is that acetic acid causes that mouse peritoneal capillary permeability changes and carrageenin causes that rat foot opens up swelling, this only can illustrate that above-mentioned composition has antiinflammatory action, and the effect of Saussurea involucrata culture aspect rheumatoid arthritis can not be described.
Summary of the invention
The object of the invention is to propose Saussurea involucrata culture and promote the application in bone health product in preparation.
Saussurea involucrata culture promotes the application in bone health product in preparation.
Saussurea involucrata culture of the present invention is to choose Herba Saussureae Involueratae callus as subculture seed, obtains by cell culture technology successive transfer culture, and Chinese patent ZL200510115902.0 has illustrated the method for Saussurea involucrata culture large-scale culture.
Further, the invention provides the application of Saussurea involucrata culture in food or the medicine of preparation promotion bone health.
Further, described promotion bone health is prevention and treatment osteoporosis.
Further, described promotion bone health is for improving calcium content of bone.
Further, described promotion bone health is for increasing bone density.
Further, described promotion bone health is for improving rheumatoid arthritis.
Further, described promotion bone health is treatment of arthritis.
Further, the administering mode of described medicine is oral, injection, transfusion or transdermal administration.
Further, the food dose of preparing with Saussurea involucrata culture be every day 0.02～4g(in Saussurea involucrata culture), preferably 0.1～0.2g.
The dose of the medicine of preparing with Saussurea involucrata culture further, be every day 0.02～12g(in Saussurea involucrata culture).
Invention effect:
Although treatment rheumatic, rheumatoid arthritis are one of traditional effects of Herba Saussureae Involueratae, but Saussurea involucrata culture of the present invention obtains (concrete cultural method refer to ZL200510115902.0) by Herba Saussureae Involueratae by plant cell culture technology, its color, character are completely different from Herba Saussureae Involueratae; Functional component composition also differs widely with Herba Saussureae Involueratae with content, and in Saussurea involucrata culture, the content of flavone, polysaccharide, chlorogenic acid, ligustrin is all far away higher than wild Herba Saussureae Involueratae.Can think, though Saussurea involucrata culture derives from Herba Saussureae Involueratae, completely different again from Herba Saussureae Involueratae.
The present invention, by animal experiment and clinical trial, finds that Saussurea involucrata culture can improve calcium content of bone, increase bone density with prevention and treatment osteoporosis, can improve rheumatoid arthritis, and then can be for promoting in the food and medicine of bone health.
Detailed description of the invention
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
BMD (bone density) is the conventional physical signs of Orthopedic Clinical, is the standard of current diagnosis osteoporosis.World Health Organization (WHO) is T value based on bone density (will check compared with institute's bone density that obtains (BMD) and normal young crowd's BMD, exceed (+) or several lower than (-) youthful standard deviation (SD) to draw) for osteoporotic definition, specific as follows:
Normal: bone density is (T value is between+1～-1) in the 1SD of youngster meansigma methods;
Low bone density: bone density lower than youthful meansigma methods 1～2.5SD(T value between-1～-2.5);
Osteoporosis: bone density lower than youthful meansigma methods 2.5SD(T value lower than-2.5);
Serious osteoporosis: bone density lower than youthful meansigma methods 2.5SD(T value lower than-2.5), with a place or many places osteoporotic fracture.
Sample: Saussurea involucrata culture capsule, every containing Saussurea involucrata culture powder 100mg;
Dose: two of every days;
Time of Administration: 6 months;
Bone density meter: dual energy X-ray absorptiometry instrument
Measuring point: distal radius
| Case | Symptom | T value before taking medicine | T value after taking medicine | Symptom is improved situation |
| 1 | Pain in the lumbar region | -3.1 | -1.9 | Pain relief |
| 2 | Back pain | -2.7 | -0.6 | Pain disappears |
| 3 | Pain in the lumbar region | -3.3 | -1.5 | Pain relief |
| 4 | Back pain | -2.5 | -0.7 | Pain disappears |
Embodiment 2
Saussurea involucrata culture is for rheumatoid arthritis clinical trial
Test method: case selection is with reference to Americanism damp disease association Diagnosis of Rheumatoid Arthritis standard, and the course of disease 6 months-11 years, takes after Saussurea involucrata culture, observes patient sign situation.
Case quantity: 15
Test period: 4 weeks
Taking dose: every day 2 times, each 100mg Saussurea involucrata culture
Result of the test: pain improves 73.3%, swelling improves 66.7%, stiff improvement 60.0% in morning.
Patient's arthralgia, swelling, stiff total effect comparison in morning after treatment
| Case number | Pain is closed joint number | ? | Curative effect | Case number | Swollen joint number | ? | Curative effect | Case number | Morning, deadlock was closed joint number | ? | Curative effect |
| ? | Before treatment | After treatment | ? | ? | Before treatment | After treatment | ? | ? | Before treatment | After treatment | ? |
| 1 | 6 | 2 | Improve | 1 | 6 | 4 | Improve | 1 | 2 | 1 | Improve |
| 2 | 6 | 2 | Improve | 2 | 6 | 2 | Improve | 2 | 2 | 2 | Invalid |
| 3 | 4 | 0 | Disappear | 3 | 4 | 0 | Disappear | 3 | 2 | 1 | Improve |
| 4 | 6 | 2 | Improve | 4 | 6 | 2 | Improve | 4 | 2 | 0 | Disappear |
| 5 | 6 | 4 | Improve | 5 | 6 | 6 | Improve | 5 | 2 | 0 | Disappear |
| 6 | 4 | 4 | Invalid | 6 | 4 | 4 | Invalid | 6 | 2 | 1 | Improve |
| 7 | 4 | 4 | Invalid | 7 | 4 | 4 | Invalid | 7 | 2 | 1 | Improve |
| 8 | 4 | 0 | Disappear | 8 | 4 | 0 | Disappear | 8 | 1 | 1 | Invalid |
| 9 | 6 | 6 | Invalid | 9 | 6 | 6 | Invalid | 9 | 1 | 0 | Disappear |
| 10 | 6 | 2 | Improve | 10 | 6 | 2 | Improve | 10 | 1 | 0 | Disappear |
| 11 | 2 | 2 | Invalid | 11 | 2 | 2 | Invalid | 11 | 2 | 2 | Invalid |
| 12 | 4 | 0 | Improve | 12 | 4 | 0 | Improve | 12 | 2 | 1 | Improve |
| 13 | 2 | 0 | Disappear | 13 | 2 | 0 | Disappear | 13 | 2 | 2 | Invalid |
| 14 | 4 | 0 | Disappear | 14 | 4 | 4 | Invalid | 14 | 1 | 1 | Invalid |
| 15 | 4 | 0 | Disappear | 15 | 4 | 0 | Disappear | 15 | 2 | 2 | Invalid |
Experimental example 1
Saussurea involucrata culture increases bone density, improves calcium content of bone, prevents and treats osteoporotic effect experiment
Materials and methods
Test grouping: sham operated rats, model control group, positive controls (estradiol 0.12mg/kg)
Test model: the low drag of removal ovary bone density
Experimental animal: the SD female rats of growing up, castration operation prospective adaptation is raised 3-5 days.
Modeling method: rats underwent bilateral oophorectomy castration, give and special feedstuff simultaneously, cause the low drag of bone density; Separately get the similar operation of part rats underwent but do not excise bilateral ovaries, only extracing part fat, being sham operated rats.
Formula feedstuff is with reference to AIN93 feed formula preparation semi-finished product feedstuff, with the composition that derives from Semen sojae atricolor in suitable substitution of materials feedstuff, to avoid the interference of the phytohormone such as soybean isoflavone to experimental result.Formula composition: casein 23%, DL-methionine 0.3%, corn starch 32%, sucrose 30%, fiber 5%, Semen Maydis oil 5%, mixed mineral salt 3.5%, mixed vitamin 1%, two (2-Hydroxyethyl)trimethylammonium bitartrate 0.2%, the feed corporation,Ltd that pulls together of Beijing Australia of section produces.
In Saussurea involucrata culture high dose group (100mg/kg.d), Saussurea involucrata culture, dosage group (33.3mg/kg.d), Saussurea involucrata culture low dose group (16.7mg/kg.d) mg/kg.d of the present invention are the day dosage of every kilogram of rat body weight.
Size of animal: 12 every group
Medication: per os gastric infusion, every day 1 time, continues 3 months.
Test item: after three months, get rat femur, measure bone calcium, bone density (femur mid point and femur distal end), ash weight and dry weight etc.
Bone calcium is measured: aas determination calcium content of bone;
Bone densitometry: by borne densitometers mensuration femur mid point and femur distal end density.
The impact of Saussurea involucrata culture on ovariectomized rat bone calcium and bone density
Note: with model group comparison, * p < 0.05, * * p < 0.01, * * * p < 0.001.
Result: after oophorectomize, model group is compared with sham operated rats, and rat bone density and calcium content of bone all have obvious reduction and have statistical significance, illustrates that Ovariectomized rats is because estrogen deficiency can cause rat bone calcium and Decrease of Bone Mineral Density, modeling success; After administration 90 days, the calcium content of bone of the each dosage group of Saussurea involucrata culture is all increased significantly compared with model group, and has statistical significance (p < 0.01 or p < 0.001); Distal femur bone density is all significantly improved compared with model group, has significant difference (p < 0.05 or p < 0.01); Each dosage group of femur mid point bone density also has in various degree to be increased, and low dose group has significant difference (p < 0.05) compared with model group.
Conclusion: Saussurea involucrata culture has the effect of obvious increase bone calcium for the low rat model of bone density, also have increasing action for femur mid point bone density and distal femur bone density.Therefore, Saussurea involucrata culture can, for prevention and treatment osteoporosis, promote bone health.
Experimental example 2
Freund's complete adjuvant rat arthritis model is one of conventional animal model of rheumatoid arthritis.This experimental example is intended to illustrate the therapeutical effect of Saussurea involucrata culture to rat assist agent arthritis, and then its therapeutical effect to rheumatoid arthritis is described.Materials and methods
Test grouping: dosage group (70mg/kg.d), Saussurea involucrata culture low dose group (35mg/kg.d) in solvent group (0.5% sodium carboxymethyl cellulose), positive controls (XUBI KELI 2g/kg, indomethacin 4.5mg/kg), Saussurea involucrata culture high dose group (140mg/kg.d), Saussurea involucrata culture
Test model: Freund's complete adjuvant rat arthritis model
The rat of experimental animal: body weight 160-200g, male and female half and half.
Modeling method: measure respectively rat both sides hind legs foot volume with sufficient volume measuring apparatus, right back sufficient plantar subcutaneous injection Freund's complete adjuvant (containing 0.5 milligram of deactivation tubercule bacillus) 0.1ml, within after injection the 15th day, measure the weight of animals, both sides hind leg foot volume, with left side (the non-inflammation foot that causes) hind leg swelling rate and body weight grouping, be divided into six groups, 10 every group.
Size of animal: 10 every group
Medication: gastric infusion, administration volume is 20ml/kg.Once a day, give seven days.
Test item and method: the body weight of every animal is measured in the 2nd, 5,8 days after administration respectively, both sides hind leg foot volume, calculates left and right pedal swelling rate.
Saussurea involucrata culture causes the impact of scorching sufficient pedal swelling on adjuvant arthritis rat
Note: with the comparison of solvent group, * p < 0.05, Student ' s-t inspection
Result shows, therapeutic gives after Herba Saussureae Involueratae histiocyte culture, and within the 5th, 8 days, Herba Saussureae Involueratae histiocyte culture is to causing the visible certain inhibitory action of scorching sufficient pedal swelling.
Saussurea involucrata culture is on the non-impact that causes scorching sufficient pedal swelling of adjuvant arthritis rat
Note: with the comparison of solvent group, * p < 0.05, * * p < 0.01, Student ' s-t inspection
Result shows, within after the Herba Saussureae Involueratae histiocyte culture administration of 70mg/kg, 140mg/kg the 5th, 8 days, causes the swelling that scorching foot causes because of delayed hypersensitivity and has significant inhibitory action non-.
Conclusion: therapeutic gives Herba Saussureae Involueratae histiocyte culture material, the Herba Saussureae Involueratae histiocyte culture of 70mg/kg, 140mg/kg caused scorching foot swelling rate at the 5th, 8 days to adjuvant arthritis rat certain inhibitory action, but there is no statistical significance; The foot swelling causing because of delayed hypersensitive reaction in the face of the anti-limb of another side (the non-inflammation foot that causes) has significant inhibitory action.
Claims (10)
1. Saussurea involucrata culture promotes the application in bone health product in preparation.
2. application according to claim 1, is characterized in that, the application of Saussurea involucrata culture in food or the medicine of preparation promotion bone health.
3. application according to claim 1 and 2, is characterized in that, described promotion bone health is prevention and treatment osteoporosis.
4. application according to claim 1 and 2, is characterized in that, described promotion bone health is for improving calcium content of bone.
5. application according to claim 1 and 2, is characterized in that, described promotion bone health is for increasing bone density.
6. application according to claim 1 and 2, is characterized in that, described promotion bone health is for improving rheumatoid arthritis.
7. application according to claim 1 and 2, is characterized in that, described promotion bone health is treatment of arthritis.
8. according to the application described in claim 1-7 any one, it is characterized in that, the administering mode of described medicine is oral, injection, transfusion or transdermal administration.
9. according to the application described in claim 1-7 any one, it is characterized in that, the food dose of preparing with Saussurea involucrata culture is 0.02～4g every day.
10. according to the application described in claim 1-7 any one, it is characterized in that, the dose of the medicine of preparing with Saussurea involucrata culture is 0.02～12g every day.
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| CN106260359A (en) * | 2016-09-18 | 2017-01-04 | 大连普瑞康生物技术有限公司 | A kind of Saussurea involucrata culture compositions Ganoderma tea and preparation method thereof |
| CN106360390A (en) * | 2016-10-26 | 2017-02-01 | 广州莱可福生物科技有限公司 | Dietary supplements capable of enhancing bone density and preventing and improving osteoarthritis and production method thereof |
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