CN103865918A - Preparation method and application of immobilized microbial flocculant - Google Patents
Preparation method and application of immobilized microbial flocculant Download PDFInfo
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- CN103865918A CN103865918A CN201410120576.1A CN201410120576A CN103865918A CN 103865918 A CN103865918 A CN 103865918A CN 201410120576 A CN201410120576 A CN 201410120576A CN 103865918 A CN103865918 A CN 103865918A
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- preparation
- water
- flocculation agent
- immobilized microorganism
- microbial flocculant
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Abstract
The invention provides a preparation method of an immobilized microbial flocculant for removing sulfate contained in underground karstic water. The preparation method comprises the following steps of firstly inoculating SRB into a liquid culture medium according to the inoculation amount of 10%-20%, and culturing under the conditions of closeness, anaerote, darkness and standing; secondarily embedding a thallus obtained by centrifugalizing fermentation liquor by using zeolite-sodium alginate to prepare the immobilized microbial flocculant. The immobilized microbial flocculant obtained by adopting the preparation method has good removal effect on treatment of the high-sulfate underground karstic water, is small in using amount and pollution-free and can be recycled. Experiments prove that the obtained immobilized microbial flocculant achieves the removal ratio of the sulfate of the underground karstic water by about 96.71%.
Description
Technical field
The present invention relates to the preparation method of immobilized microorganism flocculation agent, specifically belong to a kind of preparation method of the immobilized microorganism flocculation agent of removing vitriol in underground karst water.
Background technology
In karst water, contain higher salt amount and make it not only salty not only bitter but also puckery, cause it can not serve as tap water.And in industrial and agricultural production, karst water also can cause damage, the salting of soil heighten degree etc. of industrial equipments.Sulfate ion is the main component in karst water.At present, in removal underground karst water, the method for sulfate radical mainly contains ion exchange method, reverse osmosis method, active carbon adsorption, BaCl
2method, CaCl
2the physics and chemistry method that method and cold method etc. are traditional.The method of physics and chemistry complex operation in operational process, investment cost is high, easily causes secondary pollution.Immobilized microorganism flocculation agent has the advantages such as cost is low, simple to operate, non-secondary pollution, treatment effect is good, reaction is easily controlled, stablize, solid-liquid is easily separated and can recycle.Its principle is to utilize microorganism that sulfate ion is transformed into sulfonium ion or hydrogen sulfide, and then generates that sulphur simple substance realizes.This technology this one side of vitriol in removal karst water yet there are no research and report at present.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of immobilized microorganism flocculation agent, the immobilized microorganism flocculation agent activity that the method obtains is high, and the treatment effect that is applied to the sulfate ion in high salinity and high rigidity underground karst water is good.
The preparation method of a kind of immobilized microorganism flocculation agent provided by the invention, comprises the steps:
(1) sulphate reducing bacteria is pressed to the inoculum size of 10-20%, be seeded in liquid nutrient medium, at 28-35 ℃, airtight, anaerobism, dark, leave standstill and cultivate, 7-12d, obtains microbial fermentation solution;
(2) fermented liquid is centrifugal under 5000-6500r/min rotating speed, obtain the thalline of sulphate reducing bacteria;
(3) by weight the preferred 1:2-3 of 1:0.5-3(), by soluble in water to zeolite and sodium alginate, heating for dissolving, adds sulphate reducing bacteria thalline to stir after sterilizing, then splashes into that to contain quality quality be the preferred 3-4% of 2-5%() CaCl
2saturated boric acid solution, make it to be cross-linked into the spheroid that diameter is about 2-4mm, 3-8 ℃ of immobilization 20-30h, then washs the bead fixing with sterilized water, being fixed microbial flocculant.The immobilized microorganism flocculation agent making can be kept in physiological saline.
Described liquid culture based component is counted by weight: NH
4cl0.8-1.5, MgSO
47H
2o1.0-3.0, Na
2sO
40.5-1.5, CaCl
22H
2o0.1-0.3, Sodium.alpha.-hydroxypropionate 2-5, yeast extract paste 0.5-1.5, K
2hPO
40.3-1.0, xitix 0.05-0.3, EDTA-Fe5-20, distilled water 1000.
Described sulphate reducing bacteria was desulfovibrio desulfurican (Desulfovibrio desulfuricans) originally, separated, identifies and be preserved in photosynthetic bacterium research department by University Of Shanxi's Life Science College.This bacterial classification document source: Gong Jun, Zhang Zhaoming, Mu Yao. the separation of a strain desulfurization bacterium and desulfurization kinetics, environmental engineering journal, 2012,6(4): 1310-1314.
Desulfovibrio desulfurican (Desulfovibrio desulfuricans) has the ability of sulphate reducing, and its seed and enlarged culturing process thereof obtain active vigorous desulfovibrio desulfurican fermented liquid under airtight, anaerobism, dark, standing condition.
The immobilized microorganism flocculation agent that the present invention produces can be used for processing the karst water that sulphate content is high.
Compared with prior art, the invention has the beneficial effects as follows: the underground karst glassware for drinking water that the immobilized microorganism flocculation agent that obtains with the present invention is processed high-sulfate has good removal effect, consumption is few, pollution-free, and can reuse.The experiment proved that: the immobilized microorganism flocculation agent that the present invention obtains can reach 97.95% left and right to the vitriol clearance of underground karst water.
Embodiment
Embodiment 1
Liquid culture based component: NH
4cl1.0g, MgSO
47H
2o2.0g, Na
2sO
41.0g, CaCl
22H
2o0.1g, Sodium.alpha.-hydroxypropionate 3.5mL, yeast extract paste 1.0g, K
2hPO
40.5g, xitix 0.1g, EDTA-Fe10mL, distilled water 1000mL.
(1) under aseptic condition, get 20mL desulfovibrio desulfurican (Desulfovibrio desulfuricans) access and be equipped with in 100mL substratum, be placed in 32 ℃ of incubators, airtight, anaerobism, dark, leave standstill and cultivate 10d.
(2), by fermented liquid centrifugal 15min under 6000r/min, obtain the thalline of desulfovibrio desulfurican.
(3) take respectively zeolite 1.6g and sodium alginate 3.2g, be dissolved in 100mL water, heating for dissolving, adds SRB thalline to stir (thalline embedding amount is 15%) after sterilizing, then splash into linking agent with 10mL asepsis injector from 10cm eminence and (contain 3%CaCl
2saturated boric acid solution) in, make it to be cross-linked into the spheroid that diameter is about 3mm, put into refrigerator and continue immobilization 24h, then the bead fixing is washed with sterilized water, be kept in 8.5g ∕ L physiological saline being fixed microbial flocculant.
(4) taking 2.5g immobilized microorganism flocculation agent, to throw into 35mL sulphate content be in 1856mg/L karst water, is placed in the incubator of 32 ℃, processes 72h.Adopt Barium Chromate Spectrophotometry to measure the content of vitriol in karst water after treatment, now in karst water, sulphate content drops to 38mg/L, and clearance is 97.95%.
Embodiment 2
Liquid culture based component: NH
4cl1.5g, MgSO
47H
2o3.0g, Na
2sO
41.5g, CaCl
22H
2o0.3g, Sodium.alpha.-hydroxypropionate 5mL, yeast extract paste 1.5g, K
2hPO
41.0g, xitix 0.3g, EDTA-Fe20mL, distilled water 1000mL.
(1) under aseptic condition, get 10mL desulfovibrio desulfurican (Desulfovibrio desulfuricans) access and be equipped with in 100mL substratum, be placed in 35 ℃ of incubators, airtight, anaerobism, dark, leave standstill and cultivate 7d.
(2), by fermented liquid centrifugal 15min under 6500r/min, obtain the thalline of desulfovibrio desulfurican.
(3) take respectively zeolite 1.2g and sodium alginate 3.6g, be dissolved in 100mL water, heating for dissolving, adds SRB thalline to stir (thalline embedding amount is 10%) after sterilizing, then splash into linking agent with 10mL asepsis injector from 10cm eminence and (contain 5%CaCl
2saturated boric acid solution) in, make it to be cross-linked into the spheroid that diameter is about 2mm, put into refrigerator and continue immobilization 30h, then the bead fixing is washed with sterilized water, be kept in 8.5g ∕ L physiological saline being fixed microbial flocculant.
(4) taking 2.0g immobilized microorganism flocculation agent, to throw into 35mL sulphate content be in 1856mg/L karst water, is placed in the incubator of 35 ℃, processes 36h.Adopt Barium Chromate Spectrophotometry to measure the content of vitriol in karst water after treatment, now in karst water, sulphate content drops to 61mg/L, and clearance is 96.71%.
Embodiment 3
Liquid culture based component: NH
4cl0.8g, MgSO
47H
2o1.0g, Na
2sO
40.5g, CaCl
22H
2o0.1g, Sodium.alpha.-hydroxypropionate 2mL, yeast extract paste 0.5g, K
2hPO
40.3g, xitix 0.1g, EDTA-Fe15mL, distilled water 1000mL.
(1) under aseptic condition, get 15mL desulfovibrio desulfurican (Desulfovibrio desulfuricans) access and be equipped with in 100mL substratum, be placed in 30 ℃ of incubators, airtight, anaerobism, dark, leave standstill and cultivate 12d.
(2), by fermented liquid centrifugal 15min under 5000r/min, obtain the thalline of desulfovibrio desulfurican.
(3) take respectively zeolite 1.0g and sodium alginate 3.8g, be dissolved in 100mL water, heating for dissolving, adds SRB thalline to stir (thalline embedding amount is 5%) after sterilizing, then splash into linking agent with 10mL asepsis injector from 10cm eminence and (contain 2%CaCl
2saturated boric acid solution) in, make it to be cross-linked into the spheroid that diameter is about 4mm, put into refrigerator and continue immobilization 20h, then the bead fixing is washed with sterilized water, be kept in 8.5g ∕ L physiological saline being fixed microbial flocculant.
(4) taking 2.0g immobilized microorganism flocculation agent, to throw into 35mL sulphate content be in 1856mg/L karst water, is placed in the incubator of 30 ℃, processes 48h.Adopt Barium Chromate Spectrophotometry to measure the content of vitriol in karst water after treatment, now in karst water, sulphate content is reduced to 139mg/L, and clearance is 92.51%.
Claims (4)
1. a preparation method for immobilized microorganism flocculation agent, is characterized in that, comprises the steps:
(1) sulphate reducing bacteria is pressed to the inoculum size of 10-20%, be seeded in liquid nutrient medium, at 28-35 ℃, airtight, anaerobism, dark, leave standstill and cultivate, 7-12d, obtains microbial fermentation solution;
(2) fermented liquid is centrifugal under 5000-6500r/min rotating speed, obtain the thalline of sulphate reducing bacteria;
(3) by weight 1:0.5-4, by soluble in water to zeolite and sodium alginate, heating for dissolving, adds sulphate reducing bacteria thalline to stir after sterilizing, then splash into and contain 2-5%CaCl
2saturated boric acid solution, make it to be cross-linked into the spheroid that diameter is about 2-4mm, 3-8 ℃ of immobilization 20-30h, then washs the bead fixing with sterilized water, being fixed microbial flocculant;
Described sulphate reducing bacteria was desulfovibrio desulfurican originally;
Described liquid culture based component is counted by weight: NH
4cl0.8-1.5, MgSO
47H
2o1.0-3.0, Na
2sO
40.5-1.5, CaCl
22H
2o0.1-0.3, Sodium.alpha.-hydroxypropionate 2-5, yeast extract paste 0.5-1.5, K
2hPO
40.3-1.0, xitix 0.05-0.3, EDTA-Fe5-20, distilled water 1000.
2. a kind of preparation method who prepares immobilized microorganism flocculation agent according to claim 1, is characterized in that, described zeolite and the weight ratio 1:2-3 of sodium alginate.
3. a kind of preparation method who prepares immobilized microorganism flocculation agent according to claim 1, is characterized in that, the CaCl that contains 3-4% in described saturated boric acid solution
2.
4. the flocculation agent that a kind of preparation method who prepares immobilized microorganism flocculation agent according to claim 1 makes is in the application of processing in high-sulfate karst water.
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| CN201410120576.1A CN103865918A (en) | 2014-03-28 | 2014-03-28 | Preparation method and application of immobilized microbial flocculant |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441361A (en) * | 2015-12-29 | 2016-03-30 | 中国电建集团中南勘测设计研究院有限公司 | Method for preparing strain and microbial agent for heavy metal contaminated water treatment |
| CN110394160A (en) * | 2019-08-27 | 2019-11-01 | 哈尔滨理工大学 | The preparation method of spherical calcium alginate@FeOOH desulfurizing agent |
| CN110494552A (en) * | 2018-08-09 | 2019-11-22 | 中国石油大学(北京) | One plant of bacterial strain and its application with extremely strong sulfate reduction ability |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402485A (en) * | 2008-11-10 | 2009-04-08 | 中南大学 | Cohesive action nutrient source SRB sewage sludge immobilization particle, production and uses in treating heavy metal wastewater thereof |
-
2014
- 2014-03-28 CN CN201410120576.1A patent/CN103865918A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402485A (en) * | 2008-11-10 | 2009-04-08 | 中南大学 | Cohesive action nutrient source SRB sewage sludge immobilization particle, production and uses in treating heavy metal wastewater thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘开綦 等: "硫酸盐废水厌氧处理中限制因子的探讨", 《资源开发与市场》 * |
| 刘敏: "硫酸盐还原菌的分离鉴定及其在岩溶水中的应用", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
| 贡俊 等: "一株脱硫菌的分离及脱硫动力学", 《环境工程学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441361A (en) * | 2015-12-29 | 2016-03-30 | 中国电建集团中南勘测设计研究院有限公司 | Method for preparing strain and microbial agent for heavy metal contaminated water treatment |
| CN110494552A (en) * | 2018-08-09 | 2019-11-22 | 中国石油大学(北京) | One plant of bacterial strain and its application with extremely strong sulfate reduction ability |
| WO2020029149A1 (en) * | 2018-08-09 | 2020-02-13 | 中国石油大学(北京) | Bacterial strain having very strong sulfate reduction ability and use thereof |
| CN110494552B (en) * | 2018-08-09 | 2021-11-26 | 中国石油大学(北京) | Bacterial strain with extremely strong sulfate reducing capability and application thereof |
| CN110394160A (en) * | 2019-08-27 | 2019-11-01 | 哈尔滨理工大学 | The preparation method of spherical calcium alginate@FeOOH desulfurizing agent |
| CN110394160B (en) * | 2019-08-27 | 2022-05-03 | 哈尔滨理工大学 | Preparation method of spherical calcium alginate @ FeOOH desulfurizer |
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Application publication date: 20140618 |