CN103864499A - Mushroom 808 liquid strain cultivation medium, manufacturing method and mushroom 808 cultivation method - Google Patents
Mushroom 808 liquid strain cultivation medium, manufacturing method and mushroom 808 cultivation method Download PDFInfo
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- CN103864499A CN103864499A CN201210524840.9A CN201210524840A CN103864499A CN 103864499 A CN103864499 A CN 103864499A CN 201210524840 A CN201210524840 A CN 201210524840A CN 103864499 A CN103864499 A CN 103864499A
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Abstract
The invention discloses a mushroom 808 liquid strain cultivation medium, a manufacturing method and a mushroom 808 cultivation method, which relates to the biology technology and biological product field. The mushroom 808 liquid strain cultivation medium is prepared by the following raw materials: 220-250ml of potato juice, 100-120ml of inoculated log juice, 15-20g of husk powder, 12-15g of potassium dihydrogen phosphate, 12-15g of magnesium sulfate, 16-20g of agar, 6-10g of soybean powder, 16-20g of peptone, 7-10g of brown sugar, 6-10g of grape powder and 6-10ml of edible rapeseed oil. Compared with the prior art, the mushroom 808 liquid strain cultivation medium has the advantages of high stability and good culture effect, the fruiting time of the mushroom 808 is short, the mycelia amount can reach more than 90%, and the survival rate is 100%.
Description
Technical field
The present invention relates to a kind of biotechnology and field of biological product, particularly a kind of mushroom 808 liquid strain cultivation kind substratum, making method and mushroom 808 method of cultivation.
vocabulary of terms is explained
Mushroom 808 liquid spawns that mushroom 808 Ye body Jun Zhong – the present invention adopt;
Ma bell Shu Zhi – by potato slices add water remove slag after infusion 15-18min assorted, potato: the mass ratio of water is 1:20;
Mushroom wood juice-by trees (weedtree) be cut into remove slag after the infusion 15-20min that adds water after joint assorted, trees (weedtree): the mass ratio of water is 1:20;
Husk Fen – is commercially available;
Lin acid dihydride Jia – SILVER REAGENT;
Liu Suan Mei – SILVER REAGENT;
Qiong Zhi – SILVER REAGENT;
Huang Dou Fen – is commercially available;
Dan Bai Dong – SILVER REAGENT;
Hong Tang – is commercially available;
Pu Tao Fen – is commercially available;
Shi is commercially available with Cai Zi You –.
Background technology
Mushroom 808 product are of fine quality, output is high, be the govern-house-variety of China's export mushroom, tradition plantation labour intensity is large, and the cycle is long (about 120 days, the longest kind of time in mushroom cultivation), production cost is high, and as produced with mushroom 8001, mycelia amount is few, unstable (40% left and right mycelia amount), the cycle is long, inhomogeneous, the rate of taking over a job is low, so at present mushroom liquid bacterial is never promoted.
Summary of the invention
One of object of the present invention is just to provide a kind of mushroom 808 liquid strain cultivation kind substratum, and the 808 fruiting times of mushroom that these mushroom 808 liquid strain cultivation kind substratum go out are short, and mycelia amount reaches more than 90%, and the rate of taking over a job reaches 100%.
Technical scheme of the present invention is: a kind of mushroom 808 liquid strain cultivation kind substratum, these mushroom 808 liquid strain cultivation kind substratum are made up of following raw material: potato juice 220-250ml, mushroom wood juice 100-120ml, chaff powder 15-20g, potassium primary phosphate 12-15g, magnesium sulfate 12-15g, agar 16-20g, analysis for soybean powder 6-10g, peptone 16-20g, brown sugar 7-10g, grape powder 6-10g, edible rapeseed oil 6-10ml.
Two of object of the present invention is to provide a kind of making method of mushroom 808 liquid strain cultivation kind substratum.
Technical scheme is: a kind of making method of mushroom 808 liquid strain cultivation kind substratum, the making method of these mushroom 808 liquid strain cultivation kind substratum adopts above-mentioned raw material.
As preferably, the making method of these mushroom 808 liquid strain cultivation kind substratum comprises the following steps: by described raw material blending; Be settled to 500ml; PH value is adjusted to 8 left and right; Heated and boiled 30min; Autoclaving 30-60min; Cooling.
Three of object of the present invention is to provide a kind of mushroom 808 method of cultivation.
Technical scheme is: a kind of mushroom 808 method of cultivation, these mushroom 808 method of cultivation adopt above-mentioned mushroom 808 liquid strain cultivation kind substratum.
As preferably, these mushroom 808 method of cultivation, comprise the following steps: inoculation; Shaking culture 48-60 hour; With applicator liquid inoculation bacterium bag.
Compared with prior art, beneficial effect of the present invention:
Due to chaff powder, mushroom wood juice, can make the even combination of mycelia and chaff powder, make bacterium ball uniformity, avoid mushroom liquid strain maldistribution, the shortcoming that inoculation survival rate is low, therefore the fruiting time (growth cycle) shortened to about 60 days, and mycelia amount reaches more than 90%, the rate of taking over a job reaches 100%, produces and lays a good foundation for doctor's mould.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1 mushroom 808 making of liquid strain cultivation kind substratum and the cultivation of mushroom 808
Mushroom 808 liquid strain cultivation kind substratum are made: potato juice 220-250ml, mushroom wood juice 100-120ml, chaff powder 15-20g, potassium primary phosphate 12-15g, magnesium sulfate 12-15g, agar 16-20g, analysis for soybean powder 6-10g, peptone 16-20g, brown sugar 7-10g, grape powder 6-10g, edible rapeseed oil 6-10ml are mixed, be settled to 500ml, pH value is adjusted to 8 left and right, heated and boiled 30min, puts into and is cooled to 30 degrees Celsius after medical portable pressure kettle sterilizing 30-60min and obtains below mushroom 808 liquid strain cultivation kind substratum.
The cultivation of mushroom 808: except adopting above-mentioned mushroom 808 liquid strain cultivation kind substratum, all the other are identical with the cultivation of conventional mushroom 808.(meet mushroom 808 liquid spawn mothers and plant in the fermentor tank that mushroom 808 liquid strain cultivation kind substratum are housed, pass into sterile air 20-28 degree and cultivate 48-60 hour, treat the growth of bacterium ball evenly.The fermentor tank that mushroom 808 liquid strain cultivation kind substratum are housed with the applicator liquid connection after sterilizing accesses proper amount of strains to bacterium bag under gnotobasis).
The results are shown in Table one
Comparative example 1 (mushroom 8001)
Except liquid spawn adopts mushroom 8001, substratum adopts outside conventional medium, and all the other conditions are with embodiment 1.Conventional medium composition is:
250 grams of potatos, 25 grams of glucose, 20 grams, agar, 1000 milliliters, clear water, or 40 grams of analysis for soybean powder, 20 grams of glucose, 20 grams, agar, 1000 milliliters, clear water.
The results are shown in Table one
Table one
| Substratum | Mycelia amount | The rate of taking over a job | Growth cycle |
| Comparative example 1 | 40% | 60-70% | 120 days |
| Embodiment 1 | 90% | 100% | 60 days |
Claims (5)
1. mushroom 808 liquid strain cultivation kind substratum, these mushroom 808 liquid strain cultivation kind substratum are made up of following raw material:
Potato juice 220-250ml, mushroom wood juice 100-120ml, chaff powder 15-20g, potassium primary phosphate 12-15g, magnesium sulfate 12-15g, agar 16-20g, analysis for soybean powder 6-10g, peptone 16-20g, brown sugar 7-10g, grape powder 6-10g, edible rapeseed oil 6-10ml.
2. a making method for mushroom 808 liquid strain cultivation kind substratum, is characterized in that: the making method of these mushroom 808 liquid strain cultivation kind substratum adopts raw material claimed in claim 1.
3. the making method of mushroom 808 liquid strain cultivation kind substratum according to claim 2, is characterized in that comprising the following steps:
By described raw material blending;
Be settled to 500ml;
PH value is adjusted to 8 left and right;
Heated and boiled 30min;
Autoclaving 30-60min;
Cooling.
4. mushroom 808 method of cultivation, is characterized in that: these mushroom 808 method of cultivation adopt the mushroom 808 liquid strain cultivation kind substratum described in claim 1-3.
5. mushroom 808 method of cultivation according to claim 4, is characterized in that comprising the following steps:
Inoculation;
Shaking culture 48-60 hour;
With applicator liquid inoculation bacterium bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210524840.9A CN103864499A (en) | 2012-12-10 | 2012-12-10 | Mushroom 808 liquid strain cultivation medium, manufacturing method and mushroom 808 cultivation method |
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| CN201210524840.9A CN103864499A (en) | 2012-12-10 | 2012-12-10 | Mushroom 808 liquid strain cultivation medium, manufacturing method and mushroom 808 cultivation method |
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| CN103864499A true CN103864499A (en) | 2014-06-18 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956919A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Inoculation method for mushroom wood flour liquid strains |
| CN105145110A (en) * | 2015-07-08 | 2015-12-16 | 翔天农业开发集团股份有限公司 | Mushroom wood powder solid and liquid mixing bacterial strain inoculation method |
| CN110313348A (en) * | 2019-08-13 | 2019-10-11 | 山西省农业科学院食用菌研究所 | A kind of method of the preservation of mushroom strain section wood and killing virus |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956919A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Inoculation method for mushroom wood flour liquid strains |
| CN105145110A (en) * | 2015-07-08 | 2015-12-16 | 翔天农业开发集团股份有限公司 | Mushroom wood powder solid and liquid mixing bacterial strain inoculation method |
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| CN110313348A (en) * | 2019-08-13 | 2019-10-11 | 山西省农业科学院食用菌研究所 | A kind of method of the preservation of mushroom strain section wood and killing virus |
| CN110313348B (en) * | 2019-08-13 | 2021-04-13 | 山西省农业科学院食用菌研究所 | Method for preserving mushroom strain segment wood and killing viruses |
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Application publication date: 20140618 |