CN103861069A - Humate compound fish medicine and preparation method - Google Patents
Humate compound fish medicine and preparation method Download PDFInfo
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- CN103861069A CN103861069A CN201410085796.5A CN201410085796A CN103861069A CN 103861069 A CN103861069 A CN 103861069A CN 201410085796 A CN201410085796 A CN 201410085796A CN 103861069 A CN103861069 A CN 103861069A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 101
- 239000003814 drug Substances 0.000 title claims abstract description 99
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
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Abstract
The invention discloses a humate compound fish medicine and a preparation method, and belongs to the technical field of aquaculture. The fish medicine is prepared by the following raw materials in percentage by weight: 50%-70% of humate, 10%-30% of baked ginger, 5%-20% of hemsley rockvine root, and 0.4%-2.5% of attractant. The humate compound fish medicine can specifically inhibit pathogens, and effectively prevent and treat fish diseases, and has little toxic or side effects, no drug resistance, and no drug residues; the production cost is low, the method is simple, the effect is good, the source of raw materials is stable, and the application prospect is good in the filed of aquaculture.
Description
Technical field
The present invention relates to a kind of humate compound recipe fish medicine and preparation method, belong to technical field of aquaculture.
Background technology
How effectively anti-as everyone knows, in breeding fish and producing, cure fish diseases, be the key of success or failure of breeding fish, positive so-called " breed fish and do not send out pestilence, be rich that you are dizzy ", and the control of visible fish diseases is very important.
At present, common fish diseases mainly: fin rot disease, protozoon property gill disease, bacterial spoilage disease, cotton stomatosis etc.Above disease is mostly grown pathogenic bacteria and is caused due to water pollution, adopts the killing pathogenic bacteria such as oxytetracycline, penicillin, copper sulfate, ferrous sulfate, bleaching powder more.But these sterilizing methods can pollute water quality, the heavy metal elements such as residual chlorine, copper, ferrum, or residual antibiotics makes pathogen produce drug resistance.Therefore, conventional sterilizing methods similarly is one " double-edged sword " at present, produces certain hidden danger when sterilization.
Chinese medicine have toxic and side effects few, without the feature such as Drug resistance, drug residue free, in disease prevention, have broad application prospects.Chinese medicine preparation for fish disease prevention and cure also has report.Zhao Wen etc. are control Fish parasitic disease, developed relevant Chinese medicine preparation [Zhao Wen. the punishment nanmu that jumps. the compound Chinese herb agent .2007 of control Fish parasitic disease, CN 101040936 A]; Liu Xinzhuan etc. for control ichthyosis, developed relevant Chinese medicine preparation [Liu Xinzhuan. one is treated ichthyotic Chinese medicine .2010, CN 101843722 A].
But the medicine that above-mentioned Chinese medicine preparation uses has broad-spectrum antimicrobial effect mostly, the useful antibacterial of Fish is also together killed as bacillus subtilis, Bacillus licheniformis etc.Bacillus subtilis and Bacillus licheniformis can promote useful anaerobe growth, and produce the organic acids such as lactic acid, reduce intestinal pH value, indirectly suppress other pathogenic bacterium growth.And the pathogenic bacterium of Fish are generally Aeromonas hydrophila, water mo(u)ld, escherichia coli, cartilage coccus etc., effectively suppressing these pathogenic bacterium just can preventing and curing of fish disease.
The mankind pay much attention to the residue problem of the chemicals in food, antibiotic, sulfanilamide, pesticide, heavy metal at present, various countries have formulated strict rules, China's fresh-water fishes output accounts for No. 1 in the world, in Fishery cultivation, uses safe fish medical instrument significant.Humic acids is to contain more carboxyl, the organic high molecular polymer of phenolic hydroxyl group, there is very large specific surface area, can adsorb a large amount of solids and gaseous matter, prove very strong complexation or adsorption, heavy metal ion, ammonium nitrogen in complexation water fast, the harmful substances such as pesticide to occur with metal ion, water source is fully purified, increase dissolved oxygen in water, for good environment is created in the growth of fish, therefore in industry, agricultural production, all showed good application prospect.The more important thing is, humic acids has the effects such as anti-inflammation, antiviral, promotion wound healing, find that it all has inhibitory action to escherichia coli, bacillus pyocyaneus, Bacillus typhi etc., shown that it has inhibitory action to fish diseases pathogenic bacterium such as Aeromonas hydrophila, water mo(u)ld, escherichia coli, cartilage coccuses but there is no research.
The present invention is also using Radix Apioris Fortunei (Radix Lespedezae Buergeri), Rhizoma Zingiberis Preparatum as accessory drugs, Radix Apioris Fortunei (Radix Lespedezae Buergeri) is used for the treatment of children with high fever induced convulsions, dysentery, bronchitis, pneumonia, pharyngolaryngitis, hepatitis and viral meningitis etc., above-mentioned inflammation generally causes by infection such as antibacterial, virus, funguses, and bacteriogenic extracellular toxin and endotoxin can coup injury tissues; Virus causes necrocytosis in infected time multiplexed cell system, and therefore, Radix Apioris Fortunei (Radix Lespedezae Buergeri) has potential antiinflammatory bactericidal activity; Rhizoma Zingiberis Preparatum taste is micro-pungent, peppery, and general maror has the effects such as anti-inflammation and sterilization, expelling phlegm for arresting cough, diuretic antihypertensive, stomach invigorating row be stagnant, and therefore, Rhizoma Zingiberis Preparatum also has potential inhibitory action to fish diseases pathogenic bacterium.
Take humate, Radix Apioris Fortunei (Radix Lespedezae Buergeri), Rhizoma Zingiberis Preparatum as the prevention of compound recipe and the fish medicine for the treatment of fish diseases, there is not yet bibliographical information.
Summary of the invention
Order of the present invention is for the deficiency of fish medicine in the market, a kind of control of environmental protection and the humate compound recipe fish medicine for the treatment of fish diseases are provided, this humate compound recipe fish medicine is made by following raw material, each raw material and percentage by weight are: humate 50%-70%, Rhizoma Zingiberis Preparatum 10%-30%, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 5%-20%, phagostimulant 0.4%-2.5%, the percentage by weight sum of each component is 100%.
Humate of the present invention is the one in potassium humate, sodium humate, ammonium humate.
Phagostimulant of the present invention is the mixture obtaining after one or more materials in trimethyl glycine, alanine, vanillin, garlicin, betanin, fructose mix in any proportion.
The preparation method that another object of the present invention is to provide described humate compound recipe fish medicine, specifically comprises the steps:
(1) by dry under natural conditions after raw material Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) roguing, cleaning, after natural drying, pulverize with pulverizer, cross 80-100 mesh sieve, for subsequent use;
(2) take in proportion after Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) powder mix and obtain mixed-powder, mixed-powder is added and in deionized water, soaks 1-2h than the ratio for 1:5-1:10 in mixed-powder and deionized water quality, decoct filtered while hot after 20-40min minute, then filtering residue is added and in deionized water, decocts 10-20min than the ratio for 1:1 in filtering residue and deionized water quality, filter, merge twice filtrate;
(3) in the filtrate obtaining, add phagostimulant in step (2), be placed in 40-60 ℃ of water-bath and heat and stir 10-20min;
(4) middle drug filtrate step (3) being obtained adds in humate while hot, stirs;
(5) by the humate that contains Chinese medicine obtaining in step (4) 60-80 ℃ of oven dry, be crushed to 80-100 order with pulverizer and obtain humate compound recipe fish medicine.
Humate described in the present invention makes (with free of contamination hydrogen peroxide as oxidant) with reference to patent application 200810233669. " oxidation and degradation of brown coal is produced the method for humic acids and salt thereof " method, commercial primes humate, after heavy metal detection does not exceed standard, also can use; All the other constituents are commercially available.
The using method of humate compound recipe fish medicine of the present invention is:
The consumption of humate compound recipe fish medicine is 100g/m
3be that every cubic metre of Chi Shui uses humate compound recipe fish medicine 100g, before use, first medicinal humate compound recipe fish 10 times of water are diluted, after all dissolving, be evenly sprayed onto pond water liquid level with sprinkler again, frequency of utilization is depending on season, and general 5 days summers, once 10 days other seasons once, 3 next courses for the treatment of, after finishing the course for the treatment of, just can see obvious therapeutic effect;
Or in per kilogram fish meal, admix 10g humate compound recipe fish medicine, continuous five times, after finishing the course for the treatment of, just can see obvious therapeutic effect.
Beneficial effect of the present invention is:
(1) humate compound recipe fish medicine of the present invention is take humate and Chinese medicine as raw material, and toxic and side effects is few, has without features such as Drug resistance, drug residue free, degradables;
(2) the principal agent humate of humate compound recipe fish medicine of the present invention is a kind of good complex, and energy chelating heavy metal ion, pesticide, reduce heavy metal ion, persticide residue in water, thereby make fish body health, edible safer;
(3) the selected raw material of humate compound recipe fish medicine of the present invention has good specific aim, and specific aim suppresses fish diseases pathogenic bacterium, to other pathogenic bacteria inhibitory action in water environment a little less than, can not upset bacterium colony ecological balance;
(4) the raw materials used medicine of humate compound recipe fish medicine of the present invention is cheap, and processing technology is easy, does not relate to heavy metal or poisonous and harmful reagent, compared with the more environmental protection of traditional fish medicine.
The specific embodiment
Below by embodiment, the present invention is described in further detail, but protection scope of the present invention is not limited to described content.
Embodiment 1
Described in the present embodiment, humate compound recipe fish medicine is made by following raw material, and each raw material and percentage by weight are: sodium humate 50%, Rhizoma Zingiberis Preparatum 30%, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 18%, fructose 2%.
Described in the present embodiment, the preparation method of humate compound recipe fish medicine is as follows:
(1) by dry under natural conditions after raw material Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) roguing, Rapid Cleaning, after natural drying, pulverize with pulverizer, cross 80 mesh sieves, for subsequent use;
(2) take after Rhizoma Zingiberis Preparatum 0.3 kg, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 0.18 kg powder mixes and obtain mixed-powder, mixed-powder is added and in deionized water, soaks 1h than the ratio for 1:5 in mixed-powder and deionized water quality, decoct filtered while hot after 20min minute, then filtering residue is added and in deionized water, decocts 10min than the ratio for 1:1 in filtering residue and deionized water quality, filter, merge twice filtrate;
(3) in the filtrate obtaining, add 0.02kg fructose in step (2), be placed in 40 ℃ of water-baths and heat and stir 10min;
(4) middle drug filtrate step (3) being obtained joins in the sodium humate of 0.5kg while hot, stirs;
(5) by the humate that contains Chinese medicine obtaining in step (4) 60 ℃ of oven dry, be crushed to 80 orders with pulverizer and obtain humate compound recipe fish medicine.
The humate compound recipe fish medicine test of pesticide effectiveness that the present embodiment prepares is as follows:
(1) adopt filter paper method to measure the bacteriostasis of made humate compound recipe fish medicine, the strain of selecting is: Aeromonas hydrophila, water mo(u)ld, escherichia coli, cartilage coccus, bacillus subtilis, Bacillus licheniformis; Concrete grammar is as follows: 37 ℃ of strain and normal saline of cultivating 24h are mixed, and mixed bacteria liquid content reaches 10
5cfu/ml; Getting 0.1ml bacterium liquid is uniformly coated in Carnis Bovis seu Bubali cream protein solid culture medium, get respectively 5ul and soak through humate compound recipe fish medicine medicinal liquid, the sterilized water of the variable concentrations of dilution the aseptic filter paper sheet that diameter is 6mm, filter paper is sticked and is being coated with on bacterium culture medium, put 37 ℃ and cultivate 24h, observe antibacterial circle diameter, the bacteriostasis of preliminary judgement sample; "-" represents that inhibition zone is less than 1mm, and sample does not have bacteriostasis, and the concentration of fish medicine is diluted respectively for 40mg/ml, 60mg/ml, 80mg/ml, 100mg/ml, 120mg/ml, and experimental result is in table 1:
The extracorporeal bacteria inhibitor test result of table 1 humate compound recipe fish medicine
(2) humate compound recipe fish medicine the present embodiment being prepared is for the prevention of fish diseases, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of normal speckle fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1kg, normal nursing, the using method of humate compound recipe fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 2:
The preventive effect of table 2 humate compound recipe fish medicine
(3) treatment of humate compound recipe fish medicine fish diseases the present embodiment being prepared, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of rotten fin speckle fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1kg, normal nursing, the using method of humate compound recipe fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 3:
Table 3 fin rot disease therapeutic effect
Embodiment 2
Described in the present embodiment, humate compound recipe fish medicine is made by following raw material, and each raw material and percentage by weight are: potassium humate 62%, Rhizoma Zingiberis Preparatum 17.6%, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 20%, betanin 0.2%, vanillin 0.2%.
Described in the present embodiment, the preparation method of humate compound recipe fish medicine is as follows:
(1) by dry under natural conditions after crude drug Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) roguing, Rapid Cleaning, after natural drying, pulverize with pulverizer, cross 90 mesh sieves, for subsequent use;
(2) take after Rhizoma Zingiberis Preparatum 0.176kg, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 0.2kg powder mix and obtain mixed-powder, mixed-powder is added and in deionized water, soaks 1.5h than the ratio for 1:7 in mixed-powder and deionized water quality, decoct filtered while hot after 30min minute, then filtering residue is added and in deionized water, decocts 15min than the ratio for 1:1 in filtering residue and deionized water quality, filter, merge twice filtrate;
(3) in the filtrate obtaining, add 0.002kg betanin and 0.002kg vanillin in step (2), be placed in 50 ℃ of water-baths and heat and stir 15min;
(4) middle drug filtrate step (3) being obtained joins in the sodium humate of 0.62kg while hot, stirs;
(5) by the humate that contains Chinese medicine obtaining in step (4) 70 ℃ of oven dry, be crushed to 90 orders with pulverizer and obtain humate compound recipe fish medicine.
The humate compound recipe fish medicine test of pesticide effectiveness that the present embodiment prepares is as follows:
(1) adopt filter paper method to measure the bacteriostasis of made humate compound recipe fish medicine, the strain of selecting is: Aeromonas hydrophila, water mo(u)ld, escherichia coli, cartilage coccus, bacillus subtilis, Bacillus licheniformis, concrete grammar is as follows: 37 ℃ of strain and normal saline of cultivating 24h are mixed, and mixed liquor bacterial content reaches 10
5cfu/ml; Getting 0.1ml bacterium liquid is uniformly coated in Carnis Bovis seu Bubali cream protein solid culture medium, get respectively 5ul and soak through humate compound recipe fish medicine medicinal liquid, the sterilized water of the variable concentrations of dilution the aseptic filter paper sheet that diameter is 6mm, filter paper is sticked and is being coated with on bacterium culture medium, put 37 ℃ and cultivate 24h, observe antibacterial circle diameter, the bacteriostasis of preliminary judgement sample; "-" represents that inhibition zone is less than 1mm, and sample does not have bacteriostasis, and the concentration of bactericidal liquid is diluted respectively for 40mg/ml, 60mg/ml, 80mg/ml, 100mg/ml, 120mg/ml, and experimental result is in table 4:
The extracorporeal bacteria inhibitor test result of table 4 humate compound recipe fish medicine
(2) humate compound recipe fish medicine the present embodiment being prepared is for the prevention of fish diseases, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of speckle fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1.2kg, normal nursing, the using method of fish medicine is shown in summary of the invention, continues to use 3 times, within 20 days, observe fish diseases situation, statistical result is as shown in table 5:
The preventive effect of table 5 humate compound recipe fish medicine
(3) humate compound recipe fish medicine the present embodiment being prepared is for the treatment of fish diseases, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of cotton stomatosis speckle fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1.2kg, normal nursing, the using method of fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 6:
The cotton stomatosis therapeutic effect of table 6
Embodiment 3
Described in the present embodiment, humate compound recipe fish medicine is made by following raw material, and each raw material and percentage by weight are: ammonium humate 70%, Rhizoma Zingiberis Preparatum 10%, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 7.5%, garlicin 1%, trimethyl glycine 0.5%, alanine 1%.
Described in the present embodiment, the preparation method of humate compound recipe fish medicine is as follows:
(1) by dry under natural conditions after raw material Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) roguing, Rapid Cleaning, after natural drying, pulverize with pulverizer, cross 100 mesh sieves, for subsequent use;
(2) take after Rhizoma Zingiberis Preparatum 0.1 kg, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 0.076 kg powder mixes and obtain mixed-powder, mixed-powder is added and in deionized water, soaks 2h than the ratio for 1:10 in mixed-powder and deionized water quality, decoct filtered while hot after 20min minute, then filtering residue is added and in deionized water, decocts 10min than the ratio for 1:1 in filtering residue and deionized water quality, filter, merge twice filtrate;
Material powder adds the deionized water of 10 times of raw material weights to soak 2h, decocts filtered while hot after 40min minute, and filtering residue such as adds to decoct 20min at the water of quality again, and filtration, merges twice filtrate;
(3) in filtrate, add 0.01kg garlicin, 0.005kg trimethyl glycine, 0.01kg alanine, be placed in 60 ℃ of water-baths and heat and stir 20min;
(4) above-mentioned middle drug filtrate is joined in the sodium humate of 0.7kg while hot, stir;
(5) by the ammonium humate that contains Chinese medicine 80 ℃ of oven dry, be crushed to 100 orders with pulverizer and obtain compound recipe fish medicine.
The humate compound recipe fish medicine test of pesticide effectiveness that the present embodiment prepares is as follows:
(1) adopt filter paper method to measure the bacteriostasis of made humate compound recipe fish medicine, the strain of selecting is: Aeromonas hydrophila, water mo(u)ld, escherichia coli, cartilage coccus, bacillus subtilis, Bacillus licheniformis, concrete grammar is as follows: 37 ℃ of strain and normal saline of cultivating 24h are mixed, and mixed liquor bacterial content reaches 10
5cfu/ml; Getting 0.1ml bacterium liquid is uniformly coated in Carnis Bovis seu Bubali cream protein solid culture medium, get respectively 5ul and soak through humate compound recipe fish medicine medicinal liquid, the sterilized water of the variable concentrations of dilution the aseptic filter paper sheet that diameter is 6mm, filter paper is sticked and is being coated with on bacterium culture medium, put 37 ℃ and cultivate 24h, observe antibacterial circle diameter, the bacteriostasis of preliminary judgement sample; "-" represents that inhibition zone is less than 1mm, and sample does not have bacteriostasis, and the concentration of bactericidal liquid is diluted respectively for 40mg/ml, 60mg/ml, 80mg/ml, 100mg/ml, 120mg/ml, and experimental result is in table 7;
The extracorporeal bacteria inhibitor test result of table 7 bactericidal liquid
(2) humate compound recipe fish medicine the present embodiment being prepared is for the prevention of fish diseases, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of speckle fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1.4kg, normal nursing, the using method of fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 8:
The preventive effect of table 8 humate compound recipe fish medicine
(3) treatment of humate compound recipe fish medicine fish diseases the present embodiment being prepared, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of bacterial spoilage scab fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1.4kg, normal nursing, the using method of fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 9:
The sick therapeutic effect of table 9 bacterial spoilage
Embodiment 4
Described in the present embodiment, humate compound recipe fish medicine is made by following raw material, and each raw material and percentage by weight are: ammonium humate 66%, Rhizoma Zingiberis Preparatum 27%, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 5%, garlicin 2%
Described in the present embodiment, the preparation method of humate compound recipe fish medicine is as follows:
(1) by dry under natural conditions after raw material Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) roguing, Rapid Cleaning, after natural drying, pulverize with pulverizer, cross 100 mesh sieves, for subsequent use;
(2) take after Rhizoma Zingiberis Preparatum 0.27kg, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 0.05 kg powder mixes and obtain mixed-powder, mixed-powder is added and in deionized water, soaks 1.6h than the ratio for 1:8 in mixed-powder and deionized water quality, decoct filtered while hot after 16min minute, then filtering residue is added and in deionized water, decocts 10min than the ratio for 1:1 in filtering residue and deionized water quality, filter, merge twice filtrate;
Material powder adds the deionized water of 10 times of raw material weights to soak 2h, decocts filtered while hot after 40min minute, and filtering residue such as adds to decoct 20min at the water of quality again, and filtration, merges twice filtrate;
(3) in filtrate, add 0.02kg garlicin, be placed in 60 ℃ of water-baths and heat and stir 20min;
(4) above-mentioned middle drug filtrate is joined in the sodium humate of 0.66kg while hot, stir;
(5) by the ammonium humate that contains Chinese medicine 80 ℃ of oven dry, be crushed to 100 orders with pulverizer and obtain compound recipe fish medicine.
The humate compound recipe fish medicine test of pesticide effectiveness that the present embodiment prepares is as follows:
(1) adopt filter paper method to measure the bacteriostasis of made humate compound recipe fish medicine, the strain of selecting is: Aeromonas hydrophila, water mo(u)ld, escherichia coli, cartilage coccus, bacillus subtilis, Bacillus licheniformis, concrete grammar is as follows: 37 ℃ of strain and normal saline of cultivating 24h are mixed, and mixed liquor bacterial content reaches 10
5cfu/ml; Getting 0.1ml bacterium liquid is uniformly coated in Carnis Bovis seu Bubali cream protein solid culture medium, get respectively 5ul and soak through humate compound recipe fish medicine medicinal liquid, the sterilized water of the variable concentrations of dilution the aseptic filter paper sheet that diameter is 6mm, filter paper is sticked and is being coated with on bacterium culture medium, put 37 ℃ and cultivate 24h, observe antibacterial circle diameter, the bacteriostasis of preliminary judgement sample; "-" represents that inhibition zone is less than 1mm, and sample does not have bacteriostasis, and the concentration of bactericidal liquid is diluted respectively for 40mg/ml, 60mg/ml, 80mg/ml, 100mg/ml, 120mg/ml, and experimental result is in table 10;
The extracorporeal bacteria inhibitor test result of table 10 bactericidal liquid
(2) humate compound recipe fish medicine the present embodiment being prepared is for the prevention of fish diseases, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of speckle fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1.6kg, normal nursing, the using method of fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 11:
The preventive effect of table 11 humate compound recipe fish medicine
(3) treatment of humate compound recipe fish medicine fish diseases the present embodiment being prepared, observe the rear situation that uses, test method is: chooses at random fish pond water 40L in container, puts 5 of protozoon property gill scab fishes in a suitable place to breed, be numbered respectively 1.～5., every treaty 1.6kg, normal nursing, the using method of humate compound recipe fish medicine is shown in summary of the invention, continues to use 3 times, after 20 days, observe fish diseases situation, statistical result is as shown in table 12:
The sick therapeutic effect of the table 12 protozoon property gill
Above-mentioned data show, this humate compound recipe fish medicine is more obvious to the inhibition of fish bacterial pathogens, inhibition zone is generally greater than 4mm, belong to stronger inhibitory action, as not strong in bacillus subtilis, Bacillus licheniformis inhibitory action to other bacterium in water, practical application also shows, this compound recipe fish medicine is good to prevention and the therapeutic effect of the common fish diseases such as rotten fin, cotton mouth, bacterial spoilage, protozoon property gill disease, has a good application prospect.
Claims (4)
1. a humate compound recipe fish medicine, it is characterized in that: this fish medicine is made by following raw material, each raw material and percentage by weight are: humate 50%-70%, Rhizoma Zingiberis Preparatum 10%-30%, Radix Apioris Fortunei (Radix Lespedezae Buergeri) 5%-20%, phagostimulant 0.4%-2.5%, the percentage by weight sum of each component is 100%.
2. humate compound recipe fish medicine according to claim 1, is characterized in that: described humate is the one in potassium humate, sodium humate, ammonium humate.
3. humate compound recipe fish medicine according to claim 1, is characterized in that: described phagostimulant is the mixture obtaining after one or more materials in trimethyl glycine, alanine, vanillin, garlicin, betanin, fructose mix in any proportion.
4. the preparation method of humate compound recipe fish medicine described in claim 1, is characterized in that: specifically comprise the steps:
(1) by dry under natural conditions after raw material Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) roguing, cleaning, after natural drying, pulverize with pulverizer, cross 80-100 mesh sieve, for subsequent use;
(2) take in proportion after Rhizoma Zingiberis Preparatum, Radix Apioris Fortunei (Radix Lespedezae Buergeri) powder mix and obtain mixed-powder, mixed-powder is added and in deionized water, soaks 1-2h than the ratio for 1:5-1:10 in mixed-powder and deionized water quality, decoct filtered while hot after 20-40min minute, then filtering residue is added and in deionized water, decocts 10-20min than the ratio for 1:1 in filtering residue and deionized water quality, filter, merge twice filtrate;
(3) in the filtrate obtaining, add phagostimulant in step (2), be placed in 40-60 ℃ of water-bath and heat and stir 10-20min;
(4) middle drug filtrate step (3) being obtained adds in humate while hot, stirs;
(5) by the humate that contains Chinese medicine obtaining in step (4) 60-80 ℃ of oven dry, be crushed to 80-100 order with pulverizer and obtain humate compound recipe fish medicine.
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| CN107753901A (en) * | 2017-10-27 | 2018-03-06 | 姜家治 | A kind of grass carp red skin disease process for preparing medicine for being exclusively used in 11 24 months |
| CN107821490A (en) * | 2017-10-27 | 2018-03-23 | 姜家治 | A kind of grass carp red skin disease medicine for being exclusively used in 11 24 months |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101524430A (en) * | 2008-12-17 | 2009-09-09 | 唐小阶 | Traditional Chinese fish drug |
| CN102935166A (en) * | 2012-11-12 | 2013-02-20 | 何聪 | Chinese herbal medicine compound fish medicine |
| CN103585313A (en) * | 2013-11-26 | 2014-02-19 | 张嗣喜 | Compound Chinese-western powder for treating fish disease |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101524430A (en) * | 2008-12-17 | 2009-09-09 | 唐小阶 | Traditional Chinese fish drug |
| CN102935166A (en) * | 2012-11-12 | 2013-02-20 | 何聪 | Chinese herbal medicine compound fish medicine |
| CN103585313A (en) * | 2013-11-26 | 2014-02-19 | 张嗣喜 | Compound Chinese-western powder for treating fish disease |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107753901A (en) * | 2017-10-27 | 2018-03-06 | 姜家治 | A kind of grass carp red skin disease process for preparing medicine for being exclusively used in 11 24 months |
| CN107821490A (en) * | 2017-10-27 | 2018-03-23 | 姜家治 | A kind of grass carp red skin disease medicine for being exclusively used in 11 24 months |
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