CN103859394B - Formula of mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquid and preparation method thereof - Google Patents
Formula of mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquid and preparation method thereof Download PDFInfo
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- CN103859394B CN103859394B CN201410020635.8A CN201410020635A CN103859394B CN 103859394 B CN103859394 B CN 103859394B CN 201410020635 A CN201410020635 A CN 201410020635A CN 103859394 B CN103859394 B CN 103859394B
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- 241000241413 Propolis Species 0.000 title claims abstract description 60
- 229940069949 propolis Drugs 0.000 title claims abstract description 60
- 241000190633 Cordyceps Species 0.000 title claims abstract description 36
- 239000007788 liquid Substances 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000284 extract Substances 0.000 claims abstract description 21
- 238000001728 nano-filtration Methods 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims abstract description 12
- 238000010563 solid-state fermentation Methods 0.000 claims abstract description 12
- 239000000047 product Substances 0.000 claims abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims abstract description 10
- 238000011049 filling Methods 0.000 claims abstract description 7
- 238000007689 inspection Methods 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000004806 packaging method and process Methods 0.000 claims abstract description 7
- 240000001307 Myosotis scorpioides Species 0.000 claims abstract description 6
- 241000233866 Fungi Species 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 7
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 7
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 7
- 239000004302 potassium sorbate Substances 0.000 claims description 7
- 229940069338 potassium sorbate Drugs 0.000 claims description 7
- 235000010241 potassium sorbate Nutrition 0.000 claims description 7
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 7
- 229960002675 xylitol Drugs 0.000 claims description 7
- 235000010447 xylitol Nutrition 0.000 claims description 7
- 239000000811 xylitol Substances 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 4
- 229930003944 flavone Natural products 0.000 claims description 4
- 150000002212 flavone derivatives Chemical class 0.000 claims description 4
- 235000011949 flavones Nutrition 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 4
- 241000255789 Bombyx mori Species 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000009467 reduction Effects 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 229940023492 oral liquid product Drugs 0.000 abstract description 7
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 239000006185 dispersion Substances 0.000 abstract description 4
- 239000012530 fluid Substances 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 4
- 239000000243 solution Substances 0.000 abstract description 3
- 238000009472 formulation Methods 0.000 abstract description 2
- 241000256844 Apis mellifera Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 208000002874 Acne Vulgaris Diseases 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000208223 Anacardiaceae Species 0.000 description 1
- 241000219495 Betulaceae Species 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241000218691 Cupressaceae Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000004281 Eucalyptus maculata Species 0.000 description 1
- 239000001293 FEMA 3089 Substances 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000218641 Pinaceae Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000218998 Salicaceae Species 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 240000006698 Spigelia anthelmia Species 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 235000013871 bee wax Nutrition 0.000 description 1
- 239000012166 beeswax Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 235000019636 bitter flavor Nutrition 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000015111 chews Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000004579 marble Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
- A23L29/37—Sugar alcohols
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Polymers & Plastics (AREA)
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses formula of mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquid and preparation method thereof, the formula of this mountain, Kaohsiung cordyceps mycelia propolis oral liquid comprises by volume: mycelium aqueous solution filtered fluid: propolis nanofiltration solution=1:2, and in product, all the other compositions add according to the formulation principle of conventional oral liquid; The preparation method of this mountain, Kaohsiung cordyceps mycelia propolis oral liquid comprises the following steps: utilize mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia; The preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution; Extract supercritical CO by ultrasonic wave2Extraction, concentration technique, allocate mountain, Kaohsiung Chinese caterpillar fungus solid state fermentation mycelium and high-quality propolis; Add auxiliary material, allocate, then filling, inspection, packaging, finally prepared. Mountain, Kaohsiung of the present invention cordyceps mycelia propolis oral liquid mouthfeel is good, good dispersion, good stability, nonirritant, be easy to absorption of human body and be convenient to the propolis oral liquid product of the safe and high quality of taking and carrying feature.
Description
Technical field
The invention belongs to propolis oral liquid preparing technical field, relate in particular to mountain, a kind of Kaohsiung cordyceps mycelia honeybeeFormula of glue oral liquid and preparation method thereof.
Background technology
Propolis be honeybee for maintaining the active principle of whole Population Health, the bee colony of 5～60,000 yearCan only produce propolis 70-110 gram, be described as " purple gold ". Propolis mostly is summer, autumn, I campaignThe glue source plant of state roughly has the most seeds in Betulaceae, Salicaceae, Pinaceae, Cupressaceae and Anacardiaceae, withAnd peach, Lee, apricot, chestnut, rubber, eucalyptus and sunflower etc. Propolis is opaque solid, smooth surface orCoarse, plane of rupture is sand shape, tangent plane and marble appearance similar. Be yellowish-brown, sepia, taupe,Celadon, dirty-green, only a few is dark in black. There is charming special fragranced. Mildly bitter flavor,Slightly acid, that chews sticks to one's teeth, and pinches and can soften with hand rubbing. Temperature hardening during lower than 15 DEG C, become fragile, Yi FenBroken; Soft, the toughness of matter and plasticity 36 DEG C time; Temperature is melted into viscous fluid while reaching 60～70 DEG C, andIsolate beeswax. Proportion is different with different floristics, generally between 1.112～1.136. It is water insoluble,Be slightly soluble in turpentine oil, be partially soluble in ethanol, be very easily dissolved in EC. Be dissolved in 95% ethanol and be transparentMaroon, and have graininess precipitation. Pure propolis other impurity that do not have.
That propolis has is antibacterial, the effect of beautifying face and moistering lotion, promotion cytothesis, and this has just been doomed propolis and is protectingThe critical role of skin product industry, it not only can eliminate fungal infection, can also decompose pigment, calm down wrinkle,Delaying senility, accelerates the healing of histiocytic regeneration and wound, and the left acne scar of small pox acne is printed and also hadGood curative effect.
Recent studies proves, abundant and unique bioactivator that propolis is contained, make it have antibacterial,Anti-inflammatory, antipruritic, anti-oxidant, strengthen immune, hypoglycemic, reducing blood lipid, the several functions such as antitumor, to peopleBody has medical treatment, health-care effect widely, has now become the focus of various countries' scientific research, and has become emergingHealth products are extremely praised highly.
The mouthfeel that existing propolis oral liquid product exists is poor, and bitter taste is heavier, product stability comparisonPoor.
Summary of the invention
The object of the embodiment of the present invention be to provide mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquid formula andIts preparation method, is intended to solve the mouthfeel that existing propolis oral liquid product exists poor, pained highly seasoned, productThe problem of poor stability.
The embodiment of the present invention is achieved in that the formula of mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquid,The formula of this mountain, Kaohsiung cordyceps mycelia propolis oral liquid comprises by volume: mycelium aqueous solution filtered fluid:Propolis nanofiltration solution=1:2,2% xylitol (percentage by weight) and 0.1% potassium sorbate (weight hundredProportion by subtraction).
Another object of the embodiment of the present invention is to provide the system of mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquidPreparation Method, the preparation method of this mountain, Kaohsiung cordyceps mycelia propolis oral liquid comprises the following steps:
Utilize mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia;
The preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution;
Extract supercritical CO by ultrasonic wave2Extraction, nanofiltration concentration technique, by solid-state of mountain, Kaohsiung Chinese caterpillar fungusYeast-like fungi filament and high-quality propolis are allocated;
Add auxiliary material, allocate, then filling, sterilizing, inspection, packaging, finally prepared;
Concrete steps are:
The first step, utilizes mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia, and condition of culture is: GuIn body culture medium, the mass percent of dried silkworm chrysalis meal, wheat bran, corn flour, rice is 1:1:2:7; Solid-stateThe condition of ferment is: temperature is 25 DEG C, and initial aqueous rate is material-water ratio 1:1.5, carries out non-photo-irradiation treatment, trainingThe layer thickness of supporting base is 2cm;
Second step, the preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution, by mycelium and water volume ratio 1:30,Ultrasonic extraction 40min under 25 DEG C of conditions, extracts power 290W, Rotary Evaporators-0.1MPa, 50 DEG C of conditionsUnder, be concentrated into 1/5 volume, after 5000r/min centrifugal filtration, collect supernatant for subsequent use;
The 3rd step, by propolis raw material and the CO in supercriticality2Gas mixes, and then passes throughRegulate pressure and temperature, just the active ingredient in propolis can be pressed at supercritical CO2The size of middle solubility,Successively extract supercritical CO2Extraction conditions is pressure 33Mp, 40 DEG C of temperature, add 5% ethanol,Time 60min; Extract gained the super natural factor reduction of propolis the true qualities-golden yellow of propolis. By thisThe propolis supercritical CO obtaining under condition2Extract product, enter by extraction product and water volume ratio 1:10 waterRow extracts, and the solvent used of purifying must concentrate, remove, and water extraction is carried out to nanofiltration night concentrated, nanofiltration barPart is: solvent is water, and material temperature is 20 DEG C～40 DEG C, and operating pressure 0.59Mpa～0.79Mpa is doubly concentratedSeveral 3 times;
The 4th step, adds 2% xylitol (percentage by weight) and 0.1% potassium sorbate (weight percentThan), to allocate, then filling, sterilizing, inspection, packaging, finally prepared.
Further, the general flavone content of mountain, Kaohsiung cordyceps mycelia propolis oral liquid be greater than 3%, Cordyceps sinensis polysaccharideContent is greater than 0.5%.
Formula of mountain, Kaohsiung provided by the invention cordyceps mycelia propolis oral liquid and preparation method thereof, by superSound wave extracts, supercritical CO2Extraction, nanofiltration concentration technique, by mountain, Kaohsiung Chinese caterpillar fungus solid state fermentation mycelium withHigh-quality propolis carries out science compatibility, and adds partial supplementary material, makes the natural treasure of two tastes use, help each other,Develop that mouthfeel is good, good dispersion, good stability, nonirritant, be easy to absorption of human body and be convenient to takeWith the propolis oral liquid product of the safe and high quality of the feature of carrying, in oral liquid general flavone content be greater than 3%, wormGrass polysaccharide content is greater than 0.5%. Preparation method of the present invention is simple, easy to operate, preferably resolves existingThe mouthfeel that exists of propolis oral liquid product poor, pained highly seasoned, the problem that product stability is poor.
Brief description of the drawings
Fig. 1 is preparation method's flow process of mountain, the Kaohsiung cordyceps mycelia propolis oral liquid that provides of the embodiment of the present inventionFigure.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment,The present invention is further elaborated. Should be appreciated that specific embodiment described herein only in order toExplain the present invention, be not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
The formula of mountain, the Kaohsiung cordyceps mycelia propolis oral liquid of the embodiment of the present invention comprises by volume: myceliaBody aqueous solution filtered fluid: propolis nanofiltration solution=1:2,2% xylitol (percentage by weight) and 0.1%Potassium sorbate (percentage by weight).
As shown in Figure 1, preparation method's bag of mountain, the Kaohsiung cordyceps mycelia propolis oral liquid of the embodiment of the present inventionDraw together following steps:
S101: utilize mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia;
S102: the preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution;
S103: extract supercritical CO by ultrasonic wave2Extraction, nanofiltration concentration technique, by mountain, Kaohsiung Chinese caterpillar fungusSolid state fermentation mycelium and high-quality propolis are allocated;
S104: add 2% xylitol (percentage by weight) and 0.1% potassium sorbate (percentage by weight),Allocate, then filling, sterilizing, inspection, packaging, finally prepared.
Concrete steps of the present invention are:
The first step, utilizes mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia, and condition of culture is: GuIn body culture medium, the mass percent of dried silkworm chrysalis meal, wheat bran, corn flour, rice is 1:1:2:7; Solid-stateThe condition of ferment is: temperature is 25 DEG C, and initial aqueous rate is material-water ratio 1:1.5, carries out non-photo-irradiation treatment, trainingThe layer thickness of supporting base is 2cm;
Second step, the preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution: press mycelium and water volume ratio 1:30,Ultrasonic extraction 40min under 25 DEG C of conditions, extracts power 290W, Rotary Evaporators-0.1MPa, 50 DEG C of conditionsUnder, be concentrated into 1/5 volume, after 5000r/min centrifugal filtration, collect supernatant for subsequent use;
The 3rd step, by propolis raw material and the CO in supercriticality2Gas mixes, and then passes throughRegulate pressure and temperature, just the active ingredient in propolis can be pressed at supercritical CO2The size of middle solubility,Successively extract, by changing CO2Gas pressure and temperature, make to be dissolved in CO2In substance release go outCome, extract gained the super natural factor reduction of propolis the true qualities-golden yellow of propolis, and composition is complete,Do not contain heavy metal, there is no dissolvent residual, supercritical CO2Extraction conditions be pressure 33Mp, 40 DEG C of temperature,Add 5% ethanol, time 60min; The active component that adopts solvent bee glue purifying, will obtain under this conditionPropolis supercritical CO2Extract product, extract by extraction product and water volume ratio 1:10 water, andThe solvent used of purifying must concentrate, remove, and water extraction is carried out to nanofiltration night concentrated, and nanofiltration condition is: solventFor water, material temperature is 20 DEG C～40 DEG C, operating pressure 0.59Mpa～0.79Mpa, 3 times of cycles of concentration;
The 4th step, adds 2% xylitol (percentage by weight) and 0.1% potassium sorbate (weight percentThan), to allocate, then filling, sterilizing, inspection, packaging, finally prepared.
Principle of the present invention: by qualitative to mountain, Kaohsiung cordyceps mycelia, the main bioactive ingredients of propolis,On the basis of quantitative analysis, take into full account each main component synergy, synergy to each other in two kinds of raw materialsEffect, and by means such as sense organ judge, mouthfeel, physical and chemical index analysis, formulations, make human body to greatest extentGround absorbs the compounds such as Cordyceps sinensis polysaccharide and flavonoids, determines optimum formula and the processing technology of oral liquid, producesMouthfeel is good, good dispersion, good stability, nonirritant, be easy to absorption of human body and be convenient to take and carryEtc. the propolis oral liquid product of the safe and high quality of feature.
The present invention is by efficiently obtaining through refining (supercritical CO2Extraction, ultrasonic wave extract), concentrated (nanofiltration) skillArt, carries out science compatibility by mountain, Kaohsiung Chinese caterpillar fungus solid state fermentation mycelium and high-quality propolis, and adds partial supplementary material,Make the natural treasure of two tastes use, help each other, develops that mouthfeel is good, good dispersion, good stability, nothingExcitant, be easy to absorption of human body and be convenient to the propolis oral liquid product of the safe and high quality of features such as taking and carry.In oral liquid, general flavone content is greater than 3%, Cordyceps sinensis polysaccharide content is greater than 0.5%.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, not all at thisAny amendment of doing within bright spirit and principle, be equal to and replace and improvement etc., all should be included in the present inventionProtection domain within.
Claims (2)
1. a preparation method for mountain, Kaohsiung cordyceps mycelia propolis oral liquid, is characterized in that, the preparation method of this mountain, Kaohsiung cordyceps mycelia propolis oral liquid comprises the following steps:
Utilize mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia;
The preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution;
Extract supercritical CO by ultrasonic wave2Extraction, nanofiltration concentration technique, allocate mountain, Kaohsiung Chinese caterpillar fungus solid state fermentation mycelium and high-quality propolis;
Add 2% xylitol (percentage by weight) and 0.1% potassium sorbate, allocate, then filling, sterilizing, inspection, packaging, finally prepared;
Concrete steps are:
The first step, utilizes mountain, solid state fermentation mode fermenting and producing Kaohsiung cordyceps mycelia, and condition of culture is: in solid medium, the mass percent of dried silkworm chrysalis meal, wheat bran, corn flour, rice is 1:1:2:7; The optimum condition of solid state fermentation is: temperature is 25 DEG C, and initial aqueous rate is material-water ratio 1:1.5, carries out non-photo-irradiation treatment, and the layer thickness of culture medium is 2cm;
Second step, the preparation of mountain, the Kaohsiung cordyceps mycelia aqueous solution, by mycelium and water volume ratio 1:30, ultrasonic extraction 40min under 25 DEG C of conditions, extracts power 290W, under Rotary Evaporators-0.1MPa, 50 DEG C of conditions, be concentrated into 1/5 volume, after 5000r/min centrifugal filtration, collect supernatant for subsequent use;
The 3rd step, by propolis raw material and the CO in supercriticality2Gas mixes, and then, by regulating pressure and temperature, just the active ingredient in propolis can be pressed at supercritical CO2The size of middle solubility, successively extracts, supercritical CO2Extraction conditions is pressure 33Mp, 40 DEG C of temperature, add 5% ethanol, time 60min; Extract gained the super natural factor reduction of propolis the true qualities-golden yellow of propolis, by the propolis supercritical CO obtaining under this condition2Extract product, extract by extraction product and water volume ratio 1:10 water, and the solvent used of purifying must concentrate, remove, water extraction liquid is carried out to nanofiltration concentrated, nanofiltration condition is: solvent is water, and material temperature is 20 DEG C～40 DEG C, operating pressure 0.59Mpa～0.79Mpa, 3 times of cycles of concentration;
The 4th step, 2% xylitol (percentage by weight) and 0.1% potassium sorbate (percentage by weight), allocate, and then filling, sterilizing, inspection, packaging, finally prepared.
2. the preparation method of mountain, Kaohsiung as claimed in claim 1 cordyceps mycelia propolis oral liquid, is characterized in that, the general flavone content of mountain, Kaohsiung cordyceps mycelia propolis oral liquid is greater than 3%, Cordyceps sinensis polysaccharide content is greater than 0.5%.
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| CN1785055A (en) * | 2005-11-25 | 2006-06-14 | 南京中科集团有限公司 | Health-care food with function for improving immunity and its prepn. method |
| CN101292730A (en) * | 2006-10-27 | 2008-10-29 | 周瑞 | Cordyceps sinensis propolis capsule and method of preparing the same |
| CN101803730A (en) * | 2009-11-12 | 2010-08-18 | 天津市百奥生物技术有限公司 | Health care food with auxiliary protection role on chemical liver injury and preparation method thereof |
| CN101878870A (en) * | 2010-06-22 | 2010-11-10 | 江西汪氏蜜蜂园有限公司 | Preparation method of propolis all-components soft capsule |
| CN102174417A (en) * | 2011-02-23 | 2011-09-07 | 广东省微生物研究所 | Cordyceps sinensis mycelium solid fermentation extract and application thereof to preparing anti-fatigue product |
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| CN1785055A (en) * | 2005-11-25 | 2006-06-14 | 南京中科集团有限公司 | Health-care food with function for improving immunity and its prepn. method |
| CN101292730A (en) * | 2006-10-27 | 2008-10-29 | 周瑞 | Cordyceps sinensis propolis capsule and method of preparing the same |
| CN101803730A (en) * | 2009-11-12 | 2010-08-18 | 天津市百奥生物技术有限公司 | Health care food with auxiliary protection role on chemical liver injury and preparation method thereof |
| CN101878870A (en) * | 2010-06-22 | 2010-11-10 | 江西汪氏蜜蜂园有限公司 | Preparation method of propolis all-components soft capsule |
| CN102174417A (en) * | 2011-02-23 | 2011-09-07 | 广东省微生物研究所 | Cordyceps sinensis mycelium solid fermentation extract and application thereof to preparing anti-fatigue product |
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