CN1785055A - Health-care food with function for improving immunity and its prepn. method - Google Patents

Health-care food with function for improving immunity and its prepn. method Download PDF

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CN1785055A
CN1785055A CNA2005100956302A CN200510095630A CN1785055A CN 1785055 A CN1785055 A CN 1785055A CN A2005100956302 A CNA2005100956302 A CN A2005100956302A CN 200510095630 A CN200510095630 A CN 200510095630A CN 1785055 A CN1785055 A CN 1785055A
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mouse
health food
honeybee
ganodenna lucidum
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CN100420448C (en
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陈晓红
陈亮
华克伟
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Nanjing Zhongke Pharmaceutical Co., Ltd.
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Nanjing Zhongke Group Corp Ltd
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Abstract

A health-care food for improving immunity is prepared from propolis, ganoderma extract and cordyceps. Its preparing process is also disclosed.

Description

A kind of health food and preparation technology thereof who strengthens immunity
Technical field
The invention belongs to field of health care food, relate to a kind of health food that strengthens immunity, the invention still further relates to the preparation technology of this health food.
Background technology
The propolis resin that to be honeybee gather from positions such as the tree bud of plant, barks mixes the secretion with bodies of gland such as honeybee lingual gland, wax glands again, a kind of colloid substance that is transformed through honeybee processing.Honeybee is coated on the surface of whole honeycomb with these materials, plays anticorrosion, antioxidation on the one hand, comes bonding honeycomb on the other hand, and obstruction slit etc. can't grow numerous germs in honeycomb.Propolis can play comprehensive conditioning, the effect of balancing machine body immunity function, and put down in writing according to " China's book on Chinese herbal medicine ": propolis has good supplemental treatment effect to diabetes, high fat of blood, hypertension, tumour, polyp, multiple inflammation, germ disease etc.
Glossy ganoderma [Ganoderma Lucidum (Leyss ex Fr) kast] is called auspicious grass, polyporus lucidus again, and Li Shizhen (1518-1593 A.D.) is thought glossy ganoderma " cure mainly deafness, sharp joint is protected refreshingly, and beneficial vital essence, hard muscles and bones, good color, clothes are made light of one's life by commiting suicide not oldly for a long time, prolong life, and treat consumptive disease, control hemorrhoid ".
Over nearly 40 years, glossy ganoderma has been carried out a large amount of scientific researches and clinical practice work, confirmed glossy ganoderma composition has polysaccharide, mainly contains immunoregulatory function, and detoxifcation is arranged, effect such as protects the liver.
Glossy ganoderma has the effect of strengthening the body resistance to consolidate the constitution to human body, various pharmacological activities is arranged, all can prove from the medical treatment of successive dynasties name medical drugs and modern age is clinical, every and poor blood circulation, body weakness, premunition decline diseases associated, glossy ganoderma all has certain result of treatment to it.It is raw material that this prescription is selected Ganodenna Lucidum P.E for use, and it is rich in functional components such as polysaccharide, has the function that strengthens immunity.
Cordyceps mycelia is invigorated the lung and the kidney, and replenishing essence benefit gas, antitussive and antiasthmatic, stops blooding, reduces phlegm.To weak and sickly, old, overtired powerful benefiting action, the immunization that can improve human body arranged.
Propolis adds Ganodenna Lucidum P.E and cordyceps mycelia, has the effect that strengthens immunity, and, because the bacterium effect of supporting of propolis also plays a role to guaranteeing the quality of product.
Still do not adopt at present the health food of the raising immunity of organisms of these three kinds of raw material prescription preparations.
List of references:
1, Lin Zhibin, the modern study of glossy ganoderma, publishing house of Beijing Medical University, March calendar year 2001.
2, State Administration of Traditional Chinese Medicine, " China's book on Chinese herbal medicine ", Shanghai science tech publishing house.
Summary of the invention
The purpose of this invention is to provide a kind of health food that strengthens immunity.
A further object of the invention provides the preparation technology of above-mentioned health food.
The objective of the invention is to realize by following technical measures:
A kind of health food that strengthens immunity, its prescription contains the raw material of following weight portion:
Propolis 50-60 part, Ganodenna Lucidum P.E 30-40 part, cordyceps mycelia 5-15 part.
Described health food, wherein Ganodenna Lucidum P.E prepares by following method: glossy ganoderma adds 8-12 times of water gaging after cutting into slices, extract 2-3 time, each 4-6 hour, merge extract, concentrate the back spray-drying; The quality requirement of Ganodenna Lucidum P.E is: polysaccharide 〉=8.0%, moisture≤8.0%, ash content≤10.0%.The outward appearance of Ganodenna Lucidum P.E is the dark-brown powder, drying, and free from admixture has the intrinsic fragrance of glossy ganoderma.
Health food with enhancing immunity of the present invention can add any one auxiliary material that pharmaceutically allows, its preparation can be any one formulation that pharmaceutically allows, include but not limited to tablet, granule, capsule, oral liquid, syrup, pill etc., can also add the health food that other materials that do not weaken health food effect of the present invention are made complex function in the health food of the present invention.
The preparation technology of described health food comprises the following step:
Get cordyceps mycelia by prescription and pulverize, cross 80 mesh sieves, mix with Ganodenna Lucidum P.E, 110-130 ℃ hot air sterilization 1-2 hour, add again through pulverizing the propolis of 80 mesh sieves, mix, common process is made required preparation.
Beneficial effect of the present invention:
Zoopery of the present invention:
One, material and method
1, sample: Chinese caterpillar fungus honeybee curing capsule, the 0.25g/ grain, the human body recommended intake is content net weight 1000mg/ people/day, and lot number 20040518 is provided by Nanjing Zhongke Biochemical Technology Co., Ltd.
2, animal used as test:
The I group: 40 of 18.9-23.7g ICR healthy male mices, be divided into 4 groups at random, 10 every group, carry out dinitrofluorobenzene inducing mouse DTH test;
The II group: 40 of 18.5-23.4g healthy male mices, be divided into 4 groups at random, 10 every group, carry out Turnover of Mouse Peritoneal Macrophages and engulf the chicken red blood cell test;
The III group: 40 of 18.3-23.5g healthy male mices, be divided into 4 groups at random, 10 every group, carry out the clearance test of mouse carbon;
The IV group: 40 of 20.0-23.9g healthy male mices, be divided into 4 groups at random, 10 every group, carry out mouse antibodies cellulation and HD50 value (HC 50) test;
The V group: 40 of 20.3-23.9g BALB/C healthy male mices, be divided into 4 groups at random, 10 every group, carry out mouse lymphocyte conversion, the test of NK cytoactive that mouse ConA induces.
3, dosage:
According to everyone (by the 60kg batheroom scale) recommended intake every day is 1000mg, be equivalent to 16.7mg/d/kgbw, press respectively 5 times of human body recommended intakes, 10 times, 30 times designs low (84mg/kgbw), in (167mg/kgbw), high (500mg/kgbw) three dosage groups, other establishes control group (0mg/kgbw) and replaces being tried thing with sterilized water.Per os gives the thing that tried of mouse corresponding dosage once a day, and the continuous irrigation stomach is surveyed every enhancing immunity function index after 30 days.The mouse stomach amount is 10ml/kgbw.
4, main agents: dinitrofluorobenzene (DNFB), sheep red blood cell (SRBC) (SRBC), GPS, india ink, RPMI1640, ConA, MTT, isopropyl alcohol, SA buffer solution, Dou Shi reagent, YAC-1 cell, LDH matrix liquid.
5, key instrument: card punch, T1000 type electric animal balance, JA2003 type electronic balance, 722 type spectrophotometers, superclean bench, ELIASA, CO2gas incubator.
6, test method: the enhancing immunity function method of inspection by " health food check with assessment technique standard-2003 edition " is carried out.
6.1ConA inducing mouse SPL conversion test (mtt assay):
Each treated animal continuous irrigation stomach 30 days, animal is put to death in the cervical vertebra dislocation, and the aseptic spleen of getting places the little plate that fills an amount of aseptic Hank ' s liquid, grind spleen, make the individual cells suspension, filter, use Hank ' s liquid to wash 3 times through 200 eye mesh screens, each centrifugal 10min (1000r/min), then cell is suspended in the 1ml RPMI1640 complete culture solution, the blue dyeing counting viable count of platform phenol (should more than 95%), adjusting cell concentration is 3 * 10 6Individual/ml.Divide two holes to add in 24 culture plates cell suspension, every hole 1ml, a hole adds 75 μ l ConA liquid, and another hole compares, and puts 5%CO 2Cultivate 72h in 37 ℃ of incubators.Cultivate and finish preceding 4h, supernatant 0.7ml is inhaled in every hole gently, adds the RPMI1640 nutrient solution that 0.7ml does not contain calf serum, adds MTT (5mg/ml) 50 μ l/ holes simultaneously, continues to cultivate 4h.After cultivating end, every hole adds 1ml acid isopropyl alcohol, and the piping and druming mixing dissolves purple crystal fully.Lysate is moved in 96 well culture plates, wavelength 570nm, ELIASA is measured the optical density evaluation.
6.2 the tardy parasexuality reaction of dinitrofluorobenzene (DNFB) inducing mouse (DTH) (ear swelling method):
Each dosage group continuous irrigation stomach 30 days, every mouse cuts off belly wool with scissors, the about 3cm * 3cm of scope, 50 μ l evenly smear sensitization with 1%DNFB solution.Evenly be applied in mouse right ear (two sides) with 1%DNFB solution 10 μ l after 5 days and attack, evenly be applied in mouse left side ear (two sides) with the solution 10 μ l that do not contain DNFB simultaneously and compare.Attack back 24h cervical vertebra dislocation and put to death mouse, cut left and right sides auricular concha, take off diameter 8mm auricle, weigh with card punch.Get mouse spleen, thymus gland simultaneously and weigh, calculate dirty/body ratio.
6.3 serum hemolysin is measured:
Each dosage group continuous irrigation stomach 30 days, sheep red blood cell (SRBC) (SRBC) suspension of preparation 2% (v/v), every mouse lumbar injection 0.2ml carries out immunity, extracts eyeball after 4 days and gets blood in centrifuge tube, places 1h, and the centrifugal 10min of 2000r/min separates and collection serum.After 200 times of dilutions of serum, the OD value during by method of inspection working sample pipe and SRBC HD50.The amount of hemolysin is with HD50 value (HC 50) expression.
6.4 antibody-producting cell detects (Jerne improves slide method):
Each treated animal continuous irrigation stomach 30 days, sheep red blood cell (SRBC) (SRBC) the suspension 0.2ml of every mouse lumbar injection 2% (v/v) carries out immunity, and the dislocation of mouse cervical vertebra is put to death after 4 days, take out spleen, be placed in the little plate that fills an amount of aseptic Hank ' s liquid, grind spleen, make cell suspension, filter through 200 eye mesh screens, centrifugal (1000r/min) 10min uses Hank ' s liquid to wash 2 times, at last cell is suspended in the 5ml RPMI1640 nutrient solution, the counting cells number, adjusting cell concentration is 5 * 10 6Individual/ml.After the culture medium heating for dissolving of top layer, put 45 ℃ of water bath heat preservations, mix with Hank ' the s liquid of equivalent pH7.2-7.4,2 times of concentration, the packing small test tube, every pipe 0.5ml adds 50 μ l, 10% (v/v again in pipe, with the preparation of SA liquid) SRBC, 20 μ l splenocyte suspensions (5 * 10 6Individual/ml), rapidly mixing is poured on the slide of brushing the agarose thin layer, treat that agar solidifies after, the slide level buckled be placed on the horse, put into CO 2Hatch 1.5h in the incubator, will join in the slide frame groove after the complement dilution in 1: 10 that prepare then, after continuing to hatch 1.5h, counting hemolysis plaque number.
6.5 mouse carbon clearance test:
Each dosage group continuous irrigation stomach 30 days, every caudal vein injects the india ink (0.1ml/10gbw) of 3 times of dilutions, treats that prepared Chinese ink injects timing immediately.2min and 10min get blood 20 μ l from intraocular corner of the eyes veniplex respectively behind the injection prepared Chinese ink, and it is added to 2ml 0.1%Na 2CO 3In the solution, with 722 spectrophotometers at 600nm wavelength place's photometry density value.
6.6 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method):
Each treated animal continuous irrigation stomach 30 days, the chicken erythrocyte suspension of preparation 20% (v/v), every mouse lumbar injection 1ml, interval 30min, mouse is put to death in the cervical vertebra dislocation, it is faced upward the position is fixed on the mouse plate, abdominal skin is cut off in the center, injects physiological saline 2ml through the abdominal cavity, rotates mouse plate 1min, sucking-off abdominal cavity washing lotion 1ml then, average mark drips on 2 slides, puts into the enamel box that is lined with wet gauze, 37 ℃ of incubation 30min of dislocation, rinsing in physiological saline then, dry, fix, 4% (v/v) Giemsa-phosphate buffer dyeing 3min with 1: 1 acetone methanol solution, dry with the distilled water rinsing, light microscopic is observed down again.
6.7NK cytoactive is measured (determination of lactate dehydrogenase method):
Each treated animal continuous irrigation stomach 30 days, mouse is put to death in the cervical vertebra dislocation, the aseptic spleen of getting, place the little plate that fills an amount of aseptic Hank ' s liquid, grind spleen, make single cell suspension, filter through 200 eye mesh screens, use Hank ' s liquid to wash 2 times, centrifugal at every turn (1000r/min) 10min abandons the supernatant friendship cytoplasm is upspring, and adds 0.5ml sterilization washing 20 seconds, add 0.5ml 2 times of Hank ' s liquid and 8ml Hank ' s liquid after the splitting erythrocyte again, centrifugal (1000r/min) 10min, the RPMI1640 complete culture solution that contains 10% calf serum with 1ml is resuspended, with 1% glacial acetic acid dilution back counting, the blue dyeing counting viable count of platform phenol (should more than 95%) is 2 * 10 with RPMI1640 complete culture solution adjustment cell concentration 7Individual/ml.
24h washes target cell (YAC-1 cell) cultivations of going down to posterity 3 times with Hank ' s liquid before using before the test, is 4 * 10 with RPMI1640 complete culture solution adjustment cell concentration 5Individual/ml.Getting each 100 μ l of YAC-1 cell and splenocyte (imitating target than 50: 1) adds in U type 96 well culture plates, YAC-1 cell nature release aperture adds YAC-1 cell and each 100 μ l of nutrient solution, the maximum release aperture of YAC-1 cell adds YAC-1 cell and each 100 μ l of 1%NP40, above-mentioned every three parallel holes of all establishing are in 37 ℃, 5%CO 2Cultivate 4h in the incubator, then with 96 well culture plates with the centrifugal 5min of 1500r/min, draw at the bottom of the supernatant 100 μ l horizontalizations in 96 well culture plates in every hole, add LDH matrix liquid 100 μ l simultaneously, reaction 5min, every hole adds the HCl 30 μ l of 1mol/L, measures OD value at ELIASA 490nm place.
7, raising condition: mouse is that 18-22 ℃, relative humidity are to raise in the barrier system of 40-70% in temperature.
8, data analysis: with SPSS10.0 software each experiment initial data is carried out homogeneity test of variance, satisfy the neat data information that requires of variance and carry out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group; The data information of Non-Gaussian Distribution or heterogeneity of variance is carried out suitable variable conversion, wait to satisfy the normal state variance is neat require after, carry out statistical disposition with the data of changing gained.
Two, result:
1, Chinese caterpillar fungus honeybee curing capsule is to the influence of mouse body weight
By table 1 as seen, the initial body weight of mouse is compared with control group in basic, normal, high three dosage groups, and there are no significant for difference.The initial body weight of mouse is comparatively balanced between each group.Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, each dosage group body weight is carried out homogeneity test of variance, satisfy the requirement of homogeneity of variance, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Compared with control group by visible basic, normal, high three the dosage groups of table 2 result, there are no significant for difference.Be that Chinese caterpillar fungus honeybee curing capsule does not have influence to weight of mice.
Table 1 is respectively organized initial body weight (n=10, the x ± SD) of mouse
Immunity I group Immunity II group Immunity III group Immunity IV group Immunity V group
Control group 20.9±1.6 20.9±1.6 21.0±1.7 22.1±1.1 22.1±1.0
Low dose group 20.9±1.6 20.9±1.6 21.0±1.6 22.1±1.1 22.1±1.0
Middle dosage group 20.9±1.6 20.9±1.6 20.9±1.6 22.1±1.1 22.1±1.0
High dose group 20.9±1.7 20.9±1.6 20.9±1.6 22.1±1.2 22.1±1.1
Annotate: each is organized the P value and is 1.000.
Table 2 Chinese caterpillar fungus honeybee curing capsule is to the influence of mouse body weight (n=10, x ± SD)
Immunity I group Immunity II group Immunity III group Immunity IV group Immunity V group
Control group 28.8±4.7 28.5±3.7 30.1±5.5 29.4±2.5 29.5±2.0
Low dose group 29.0±3.7 30.1±3.8 29.9±4.7 30.6±1.7 30.1±1.1
Middle dosage group 28.2±4.2 29.3±4.9 31.5±4.1 29.7±1.7 29.2±2.9
High dose group 26.3±4.2 31.8±4.4 29.8±5.8 29.6±2.7 30.2±1.8
Annotate: each organizes P>0.05.
2, Chinese caterpillar fungus honeybee curing capsule is to the influence of thymus gland, spleen organ
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, the chest of being measured/body ratio, spleen/body ratio carry out normal distribution, homogeneity test of variance, satisfy the requirement of normal distribution, homogeneity of variance, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Compared with control group by visible basic, normal, high three the dosage groups of table 3 result, there are no significant for difference.
Table 3 Chinese caterpillar fungus honeybee curing capsule is to the influence of thymus gland, spleen organ (n=10, x ± SD)
Group Thymus gland/body weight (mg/g) P Spleen/body weight (mg/g) P
Control group 141±0.69 5.43±1.49
Low dose group 1.00±0.23 0.099 6.06±1.38 0.674
Middle dosage group 1.11±0.37 0.300 6.45±1.68 0.316
High dose group 1.03±0.28 0.143 6.00±1.46 0.736
3, the influence that transforms of Chinese caterpillar fungus honeybee curing capsule mouse spleen lymphocyte that ConA is induced
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, institute's measured value is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Add the ConA hole by visible basic, normal, high three the dosage group mouse of table 4 result and be significantly higher than control group with the difference that does not add ConA hole absorbance.
The influence that the mouse spleen lymphocyte that table 4 Chinese caterpillar fungus honeybee curing capsule is induced ConA transforms (n=10, x ± SD)
Group Add ConA hole and the difference that does not add ConA hole absorbance P
Control group 0.029±0.016
Low dose group 0.062±0.035 0.015
Middle dosage group 0.072±0.025 0.001
High dose group 0.068±0018 0.004
4, Chinese caterpillar fungus honeybee curing capsule is to the influence of DNFB inducing mouse DTH
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, institute's measured value is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Be significantly higher than control group by the weightening finish of the visible high dose group mouse of table 5 result auricular concha.
Table 5 Chinese caterpillar fungus honeybee curing capsule is to the influence of DNFB inducing mouse DTH (n=10, x ± SD)
Group The auricular concha weightening finish P
Control group 5.0±3.2
Low dose group 5.8±2.2 0.871
Middle dosage group 7.6±2.9 0.129
High dose group 9.6±3.2 0.003
5, Chinese caterpillar fungus honeybee curing capsule is to the influence of mice serum hemolysin formation
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, institute's measured value is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Be significantly higher than control group by the visible high dose group mice serum of table 6 result hemolysin content.
The influence that table 6 Chinese caterpillar fungus honeybee curing capsule forms the mice serum hemolysin (n=10, x ± SD)
Group HC 50 P
Control group 56±43
Low dose group 107±54 0.082
Middle dosage group 108±57 0.076
High dose group 123±50 0.017
6, the influence of Chinese caterpillar fungus honeybee curing capsule antagonist cellulation (hemolysis plaque number)
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, institute's measured value is carried out normal distribution, homogeneity test of variance, satisfy the normal distribution requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Be higher than control group by visible middle and high two the dosage group mouse hemolysis plaque digital display works of table 7 result.
Table 7 Chinese caterpillar fungus honeybee curing capsule is to the influence of mouse antibodies cellulation (hemolysis plaque number) (n=10, x ± SD)
Group Hemolysis plaque number (individual/10 6Splenocyte) P
Control group 119±15
Low dose group 163±53 0.217
Middle dosage group 204±61 0.006
High dose group 208±80 0.004
7, Chinese caterpillar fungus honeybee curing capsule is cleaned up the influence of effect to mouse carbon
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, institute's measured value is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Compared with control group by visible basic, normal, high three the dosage groups of table 8 result, there are no significant for difference.
Table 8 Chinese caterpillar fungus honeybee curing capsule is cleaned up influence (n=10, the x ± SD) of effect to mouse carbon
Group Phagocytic index a P
Control group 4.48±1.66
Low dose group 4.80±1.22 0.893
Middle dosage group 4.46±1.26 1.000
High dose group 4.42±0.64 0.999
8, Chinese caterpillar fungus honeybee curing capsule is engulfed the phagocytic rate of chicken red blood cell and the influence of phagocytic index to Turnover of Mouse Peritoneal Macrophages
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, the phagocytic rate of measuring, phagocytic index are carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Compared with control group by visible basic, normal, high three the dosage groups of table 9 result, there are no significant for difference.
Table 9 Chinese caterpillar fungus honeybee curing capsule is engulfed the phagocytic rate of chicken red blood cell and the influence of phagocytic index (n=10, x ± SD) to Turnover of Mouse Peritoneal Macrophages
Group Phagocytic rate (%) P Phagocytic index P
Control group 20.3±4.7 0.24±0.07
Low dose group 23.3±9.2 0.617 0.30±0.11 0.322
Middle dosage group 22.9±5.7 0.710 0.28±0.08 0.587
High dose group 24.1±5.9 0.438 0.31±0.06 0.174
9, Chinese caterpillar fungus honeybee curing capsule is to the influence of NK cells in mice activity
Per os gives the Chinese caterpillar fungus honeybee curing capsule 30 days of mouse various dose, institute's measured value is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, carried out statistical disposition with the comparative approach in twos of mean between one-way analysis of variance method and a plurality of experimental group and control group.Be significantly higher than control group by the visible high dose group NK cells in mice of table 10 result activity.
Table 10 Chinese caterpillar fungus honeybee curing capsule is to the influence of NK cells in mice activity (n=10, x ± SD)
Group NK cytoactive (%) P
Control group 31.1±8.2
Low dose group 35.1±6.8 0.496
Middle dosage group 26.1±6.9 0.296
High dose group 42.1±7.4 0.006
The specific embodiment
The invention will be further elaborated by the following examples.
Raw material sources that adopt among the embodiment and quality standard explanation:
Ganodenna Lucidum P.E prepares by following method: glossy ganoderma adds 8-12 times of water gaging after cutting into slices, extract 2-3 time, and each 4-6 hour, merge extract, concentrate the back spray-drying; The quality requirement of Ganodenna Lucidum P.E is: polysaccharide 〉=8.0%, moisture≤8.0%, ash content≤10.0%.Outward appearance is the dark-brown powder, drying, and free from admixture has the intrinsic fragrance of glossy ganoderma.
Embodiment 1
Get propolis 55g, Ganodenna Lucidum P.E 35g, cordyceps mycelia 10g, cordyceps mycelia is pulverized, and crosses 80 mesh sieves, mix with Ganodenna Lucidum P.E, 110-130 ℃ hot air sterilization 1-2 hour, add again through pulverizing the propolis of 80 mesh sieves, add capsule auxiliary material commonly used, mix, make 1000 capsules by the capsule common process.
Embodiment 2
Propolis 50g, Ganodenna Lucidum P.E 40g, cordyceps mycelia 10g, cordyceps mycelia is pulverized, and crosses 80 mesh sieves, mix with Ganodenna Lucidum P.E, 110-130 ℃ hot air sterilization 1-2 hour, add again through pulverizing the propolis of 80 mesh sieves, add tablet auxiliary material commonly used, mix, make 1000 by the tablet common process.
Embodiment 3
Propolis 60g, Ganodenna Lucidum P.E 30g, cordyceps mycelia 10g, cordyceps mycelia is pulverized, and crosses 80 mesh sieves, mix with Ganodenna Lucidum P.E, 110-130 ℃ hot air sterilization 1-2 hour, add again through pulverizing the propolis of 80 mesh sieves, add granule auxiliary material commonly used, mix, make 1000 bag particles by the granule common process.
Embodiment 4
Propolis 55g, Ganodenna Lucidum P.E 30g, cordyceps mycelia 15g, cordyceps mycelia is pulverized, and crosses 80 mesh sieves, mix with Ganodenna Lucidum P.E, 110-130 ℃ hot air sterilization 1-2 hour, add again through pulverizing the propolis of 80 mesh sieves, add capsule auxiliary material commonly used, mix, make 1000 capsules by the capsule common process.

Claims (5)

1, a kind of health food that strengthens immunity is characterized in that filling a prescription and contains the raw material of following weight portion:
Propolis 50-60 part, Ganodenna Lucidum P.E 30-40 part, cordyceps mycelia 5-15 part.
2, health food according to claim 1, it is characterized in that Ganodenna Lucidum P.E prepares by following method: glossy ganoderma adds 8-12 times of water gaging after cutting into slices, extract 2-3 time, each 4-6 hour, merge extract, concentrate the back spray-drying; The quality requirement of Ganodenna Lucidum P.E is: polysaccharide 〉=8.0%, moisture≤8.0%, ash content≤10.0%.
3, health food according to claim 1 is characterized in that its preparation is any one formulation that pharmaceutically allows
4, health food according to claim 3 is characterized in that its formulation is tablet, granule, capsule, oral liquid, syrup or pill.
5, the preparation technology of health food as claimed in claim 1 is characterized in that comprising the following step:
Get cordyceps mycelia by prescription and pulverize, cross 80 mesh sieves, mix with Ganodenna Lucidum P.E, 110-130 ℃ hot air sterilization 1-2 hour, add again through pulverizing the propolis of 80 mesh sieves, mix, common process is made required preparation.
CNB2005100956302A 2005-11-25 2005-11-25 Health-care food with function for improving immunity and its prepn. method Expired - Fee Related CN100420448C (en)

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CN100452991C (en) * 2006-09-13 2009-01-21 聂承和 Health care food with enhancing human body immunity, fatigue resistant and radioresistant function and making method thereof
CN103230003A (en) * 2013-04-22 2013-08-07 吉林修正药业新药开发有限公司 Health food with immunity boosting function and preparation method thereof
CN103859394A (en) * 2014-01-16 2014-06-18 安徽工程大学 Formula of geohiong mountain cordyceps mycelium propolis oral liquid and preparation method thereof
CN105012780A (en) * 2015-07-03 2015-11-04 广西大学 Coix seed oil health care capsule
CN105638931A (en) * 2016-01-07 2016-06-08 伊国群 Compound natural and nutritional health product capable of enhancing immunity
CN106666592A (en) * 2016-12-23 2017-05-17 程艳青 Health product for old people with effects for supplementing calcium, and regulating blood lipid and blood pressure
CN108653569A (en) * 2018-06-26 2018-10-16 吕军 It is a kind of for kidney tonifying, sleeping, toxin expelling health products and preparation method thereof

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100452991C (en) * 2006-09-13 2009-01-21 聂承和 Health care food with enhancing human body immunity, fatigue resistant and radioresistant function and making method thereof
CN103230003A (en) * 2013-04-22 2013-08-07 吉林修正药业新药开发有限公司 Health food with immunity boosting function and preparation method thereof
CN103230003B (en) * 2013-04-22 2015-01-07 吉林修正药业新药开发有限公司 Health food with immunity boosting function and preparation method thereof
CN103859394A (en) * 2014-01-16 2014-06-18 安徽工程大学 Formula of geohiong mountain cordyceps mycelium propolis oral liquid and preparation method thereof
CN103859394B (en) * 2014-01-16 2016-05-25 安徽工程大学 Formula of mountain, a kind of Kaohsiung cordyceps mycelia propolis oral liquid and preparation method thereof
CN105012780A (en) * 2015-07-03 2015-11-04 广西大学 Coix seed oil health care capsule
CN105638931A (en) * 2016-01-07 2016-06-08 伊国群 Compound natural and nutritional health product capable of enhancing immunity
CN106666592A (en) * 2016-12-23 2017-05-17 程艳青 Health product for old people with effects for supplementing calcium, and regulating blood lipid and blood pressure
CN108653569A (en) * 2018-06-26 2018-10-16 吕军 It is a kind of for kidney tonifying, sleeping, toxin expelling health products and preparation method thereof

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