CN103849622A - Plant cold-induced expression promoter Poscold 2 and application thereof - Google Patents
Plant cold-induced expression promoter Poscold 2 and application thereof Download PDFInfo
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- CN103849622A CN103849622A CN201410119971.8A CN201410119971A CN103849622A CN 103849622 A CN103849622 A CN 103849622A CN 201410119971 A CN201410119971 A CN 201410119971A CN 103849622 A CN103849622 A CN 103849622A
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Abstract
The invention provides a plant cold-induced expression promoter Poscold 2 and application thereof. The invention further provides an expression cassette including the promoter, a plant expression vector, a host bacterium and a converter. Specifically, the invention obtains the promoter and applies the promoter in plant transgene engineering. Promoter provided by the invention can drive an exogenous gene to express in the plant under a cold-inducing condition, is suitable for monocotyledon cereal plants, particularly capable of driving the exogenous gene to express in a rice plant, and therefore, the plant cold-induced expression promoter Poscold 2 can be used for improving growth characteristics of rice, so that an ideal cold-resistant rice variety is cultivated.
Description
Technical field
The present invention relates to biotechnology and plant gene engineering technology field.Particularly, the present invention relates to the cold inducible gene expression promotor of a kind of plant and application thereof, this promotor can drive target gene to express in plant in Transgenic Rice adjustment and control system under cold inductive condition.
Background technology
Paddy rice is important food crop, belongs to the warm crop of happiness, more responsive to low-temp reaction, when temperature is during lower than a certain critical temperature, rice growth will be received and suppress or harm, i.e. chilling injury, therefore, damaging to plants caused by sudden drop in temperature is one of critical limitation factor affecting Rice Production.According to different on paddy rice impact, Japanese Rice expert is divided into delaying type, obstruction type, rice blast type, mixed cold harm damaging to plants caused by sudden drop in temperature.Paddy rice all may be damaged to plants caused by sudden drop in temperature from rudiment to each ripe period, worldwide, high latitude, high altitude localities generally occur because chilling injury causes the phenomenon of the paddy rice underproduction, in China with the Northeast, southwest is outstanding especially, China is every year because of about 30-50 hundred million kg of chilling injury underproduction paddy, and loss is serious.Therefore in the urgent need to excavating rice cold tolerance germ plasm resource, cultivate rice cold tolerance kind.
So far, researchist both domestic and external has carried out insistent exploration and research, has carried out the anti-cold breeding of new variety work of coercing, and has obtained certain achievement.Zhong Kang etc. (2007), by the proline content rising in overexpression render transgenic paddy rice in paddy rice of zinc finger protein albuminoid OsCOIN gene, improve the resistance to cold of plant.Zhang Hongsheng etc. (2009) are by paddy rice zinc finger protein ZFP245 gene overexpression in paddy rice, find that overexpression ZFP245 trans-genetic hybrid rice makes the expression amount of Proline Accumulation genes involved OsP5CS and proline(Pro) transporter gene OsProT gene improve under cold coercing, impel the amount of proline(Pro) to increase, strengthen the resistance to cold of plant.Remaining virtuous and beautiful grade (2010) is found can survive after MYBS3 overexpression paddy rice can be processed under 4 ℃ of conditions, and does not affect its Other Main Agronomic Characters.In addition, some are comprised to the overexpression of the transcription factor such as DREB/CBF, NAC can improve the resistance to cold of paddy rice.
At present, along with the develop rapidly of molecular biology, information biology and reaching its maturity of transgenic technology, carry out molecular breeding by modern genetic engineering technology and become a kind of high effective way of improvement rice cold tolerance.
China's paddy rice aboundresources, excavates, locates, clones cold-resistant genes involved and cold induced promoter thereof from rice seed, will be significant to Rice Production.
Summary of the invention
The object of this invention is to provide a kind of drive foreign gene under cold inductive condition specific expressed promotor, obtain the transformant that contains this promoter sequence and the application of this promotor.Wherein, related " plant " refers to monocotyledons herein, and for example paddy rice, wheat, corn, barley, Chinese sorghum or oat, be preferably paddy rice.
To achieve these goals, on the one hand, the invention provides the cold abduction delivering promotor of kind of plant Poscold2, the cold abduction delivering promotor of described plant Poscold2 comprises the DNA sequence dna shown in SEQ ID No:1 in sequence table.In sequence table, the DNA sequence dna shown in SEQ ID No:1, for deriving from the Rice Cold abduction delivering promotor of Japanese fine paddy rice (Oryza sativa L cv.Nipponbare), is called Poscold2 or promotor Poscold2 herein.
Preferably, the DNA sequence dna of the cold abduction delivering promotor of plant provided by the invention Poscold2 is the sequence shown in SEQ ID No:1, i.e. Poscold2 or promotor Poscold2.
On the other hand, the invention provides the cold abduction delivering promotor of kind of plant Poscold2, the DNA sequence dna shown in its DNA sequence dna and SEQ ID No:1 has at least 80% homology; Or the cold abduction delivering promotor of described plant Poscold2 add, replace, insert or delete mutant or allelotrope or the derivative that one or more Nucleotide generate in the DNA sequence dna shown in SEQ ID No:1; Or the cold abduction delivering promotor of described plant Poscold2 has the product with the DNA sequence dna hybridization shown in SEQ ID No:1.DNA sequence dna shown in the cold abduction delivering promotor of these plants Poscold2 sequence and SEQ ID No:1 has identical function, drives target gene specific expressed in plant under cold inductive condition.
The cold induction that the present invention mentions refers to the low temperature (for example, 4 degrees Celsius) that transfer-gen plant is applied to for some time, thereby expresses by promotor induction specific gene of the present invention.
On the other hand, the present invention also provides a kind of expression cassette that comprises the cold abduction delivering promotor of above-mentioned plant Poscold2.
Another aspect, the present invention also provides a kind of recombinant expression vector, described recombinant expression vector comprises the cold abduction delivering promotor of above-mentioned plant Poscold2, and in described recombinant expression vector, the cold abduction delivering promotor of described plant Poscold2 is connected in the upstream of gene order to be expressed; Preferably, described gene to be expressed is Gus gene, described recombinant expression vector is pCAMBIA1391-Poscold2, this recombinant expression vector is to be that Poscold2 or promotor Poscold2 are implemented in the recombinant expression vector obtaining in pCAMBIA1391 by the sequence shown in SEQ ID No:1, is called pCAMBIA1391-Poscold2 herein.Or, in actual applications, select cold tolerance gene as gene to be expressed, under cold inductive condition, drive cold tolerance gene to express by promotor of the present invention, thereby improve the resistance to cold of plant.
On the other hand, the present invention also provides a kind of Host Strains, and described Host Strains comprises the cold abduction delivering promotor of above-mentioned plant provided by the invention Poscold2, above-mentioned expression cassette or above-mentioned recombinant expression vector; Preferably, described Host Strains is agrobacterium tumefaciens.
On the other hand, the invention provides a kind of transformant, described transformant comprises the cold abduction delivering promotor of above-mentioned plant provided by the invention Poscold2, above-mentioned expression cassette, above-mentioned recombinant expression vector.Wherein, described transformant is preferably transgenic cell line, callus or plant.
Again on the one hand, the invention provides the cold abduction delivering promotor of above-mentioned plant Poscold2 in the application of cultivating in transgenic plant.Described application comprise the cold abduction delivering promotor of above-mentioned plant provided by the invention Poscold2 is connected in to carrier gene order upstream to be expressed (for example, before described promoter sequence is placed in to target gene), thereby structure recombinant expression vector, is transformed into described recombinant expression vector in vegetable cell, tissue or organ and cultivates.Described gene to be expressed preferably can be for plant provides resistance to cold, i.e. cold tolerance gene.
And preferably, described application can be for improving plant growth characteristic, and described plant is monocotyledons, and for example paddy rice, wheat, corn, barley, Chinese sorghum or oat, be preferably paddy rice.
The DNA sequence dna of the promotor providing in the present invention is (with identical in SEQ ID No:1 in sequence table):
tccagttgat taccatcaga tgacacatga acatcaggat cagccgggag tgcacgtgat 60
tgtgccacca tgggtccatg gctgttttgg acacattgcg tgtgacgacg cccctcaatc 120
atctcaaacc tgatcaggtt ttctcaccgg agatgctcct atgatggctg tttggaataa 180
ccaggtagga gtagtactag tactacccaa gtggtgcacg tggcatggtg cgcctgacag 240
gtcgggccca cgggtcacga tccacatcgc ccccgaaaaa ggtcagtgga gaaaacaatg 300
agcggttgag cgcaaaacat gtggccgagg cgtgtctcgg agccctgcag cgccaacccg 360
taagcttgtg gccagggata gtccatttgt tcctctcacg gtggtacgga gaagagaaca 420
acaaacagcg ggttctctcc ctgctgtgcg cctctggttg ggcgacatgt ggcgggcggt 480
gagttggcag ctgcgtgtct cggtggcttt gacgtgtcca aaaagcgagc gggtgggggc 540
cccctcctcc gctgcgcttt tcttttcttt ctatccatcc aaacagggac agtcctgtct 600
cctgtgccta gatttgcatg tcgccagata cgtctcctgt actcccttcg actacctccg 660
tccaaaaata taataacttt ttactataaa tctggacaca catgtgtcca aatttatagc 720
taaaaatgct tacatttttt tatggaggga gtatttcaat tgcagccatg attttccgtg 780
tctaacttta accgtctgtt ttatttaatt ttttttaaaa aaataaaaac ataagtcagg 840
agtaaagtat tgtttatatt ttatcatctc ataaaaaaaa ctaattacaa aattttttta 900
aataagatgg acgatcaaat tatgatcgca cttaaaataa aacggaggga gtagaagaga 960
aaaagctcaa taattcgtgt agtaccccga aagccagccg aggtgacatc accgcagttc 1020
tgctcatagt ttgatccaaa acagttttag agtttgttct cactactaag ttcaaccccc 1080
caagatattt tcattctttg cttgccaaaa atcatcagca gaaaaaaaca tgaactgctt 1140
gttaattcga tctataccaa ccgaagttta cctgaaactc tgataactgc agcctatcat 1200
gctccaccag ctgccagggc aattatggta ctatctccag agcacgcaca cagtaaatag 1260
cagcctaaca tccttcgatc ttatcgtcgg caaaacacac acgcatagag tggataggat 1320
cacaggaaca tgccatcgat tcgtgaagac ccacgaaagc gcacggtttt actcctacac 1380
ccacatacta gtagtagtag cggtagcagt acggtacatg tggctagtac tagcagtaat 1440
gcttggtccg gagcagcgag ccgtgtggtt gctgcagtgt gcgagtagcc aactagtggc 1500
aaccagacac atggacaagt agcagcgaag caggcgaagg aaggggcaag tgagtgccgt 1560
gaagcagcga gagccacaag aatcgagcgg cttccggggc tctctacagg attgaggcga 1620
aaagtgcaaa gccggaaagg gagagagaga aaaaagcgta gaaaaatccg atccgacgtg 1680
gcgccctggc ctgtgccctt tttctcctct ctcctgcctc ctcttggtct catttcccct 1740
tcctgccttg cctcctcgtc tccttctctg ctgctctcca agctcttcct gcttccatca 1800
ggttgtctcg agaaagattg gagtttttgg tggtttgggt tgtgcggttc ttgcttttgt 1860
tggcgcaagc gcatggctgg ttctgggaca tagtgagctg agatagagtt cttgtgttcg 1920
gtggagtttg gctttggtga atcttcgatt caagtctgga g 1961
It should be noted that: in the DNA sequence dna of above-mentioned promotor, the retention sequence of the forward primer that the sequence " tccagttgat taccatcaga tg " that sequence beginning represents take italic overstriking is used in obtaining promotor process, 22bp altogether; The retention sequence (the corresponding sequence complementation of this retention sequence and reverse primer) of the reverse primer that the sequence " a atcttcgatt caagtctggag " that sequence end represents take italic overstriking is used in obtaining promotor process, altogether 22bp; In this DNA sequence dna, remaining part is available from the DNA sequence dna in the fine paddy rice of Japan.It is emphasized that the promotor mentioned both can refer to above-mentioned whole DNA sequence dna herein, also can refer to remove above-mentioned primer and retain the DNA sequence dna after sequence.
In sum, the present inventor's separating clone from the fine paddy rice of Japan (Oryza sativa L cv.Nipponbare) obtains the DNA sequence dna of the 1961bp of structure including transcription initiation site, and by the SEQ ID No:1 in its called after Poscold2(sequence table).This sequence after cutting, enzyme is connected on plant binary expression vector pCAMBIA1391, obtain corresponding recombinant plasmid (being recombinant expression vector), utilize this recombinant plasmid transformed agrobacterium tumefaciens bacterial strain EHA105, then carry out the conversion of paddy rice by agriculture bacillus mediated method, obtain transgenic rice plant.The transgenic paddy rice obtaining is carried out to histological chemistry and detect discovery, transfer-gen plant is after cold induction is processed, the relatively high and aobvious blueness of Gus gene expression dose on the whole, thereby the sequence that proves this 1961bp has the activity that drives genetic expression, and the Gus gene of this promoters driven is specific expressed after Rice Cold induction is processed.
Promoter sequence of the present invention can be connected with plant binary expression vector, for replacing constitutive promoter.And this promoter sequence can link with required target gene, build recombinant plant expression vector, through transforming, after cold induction is processed, can drive target gene specific expressed in plant, thereby improve the expression amount of external source target gene in plant, increase genetically modified effect.In the time of concrete application, target gene can be chosen as and there is the gene (cold tolerance gene) that improves plant resistance to cold function, like this, after processing by cold induction, this gene great expression, strengthens the resistance to cold of plant.
Technique effect
The rice starter Poscold2 that the present invention clones can concentrate and express in good time by regulatory gene in plant, has in actual applications remarkable value.By this promotor, variety of crops is carried out to genetic modification, as expressed in plant by this promoter regulation target gene, replace the constitutive promoters such as 35S, thereby cultivate the cold-resistant transgenic plant kind that desirable biological safety is high.
Accompanying drawing explanation
Below, describe by reference to the accompanying drawings embodiment of the present invention in detail, wherein:
Poscold2 promotor is implemented in the schematic diagram in pCAMBIA1391 vector plasmid by Fig. 1, wherein in Fig. 1, A is pCAMBIA1391 schematic diagram, in Fig. 1, B is pCAMBIA1391-Poscold2 schematic diagram, wherein shows the gus gene that utilizes Poscold2 promoters driven to be positioned at its downstream and expresses;
Fig. 2 is the Poscold2::GUS transfer-gen plant tissue staining figure sprouting after 21 days.The rice plant of normal growth under 28 ℃ of conditions, after dyeing in 24 hours, root (A), stem (B), leaf (C) is all without GUS activity, and 4 ℃ of deepfreezes after 24 hours, then after 30min dyeing, at root (D), stem (E), all has GUS strong expression (scale=10mm) in leaf (F).
Fig. 3 is the result schematic diagram of promotor of the present invention being carried out enzyme and cut checking.
Embodiment
Referring to specific embodiment, the present invention is described.It will be appreciated by those skilled in the art that these embodiment are only for the present invention is described, the scope that it does not limit the present invention in any way.
Experimental technique in following embodiment, if no special instructions, is ordinary method.Medicinal raw material, reagent material etc. used in following embodiment, if no special instructions, is commercially available purchase product.
The acquisition of the Poscold2 promotor that contains restriction enzyme site
The design of step 1, primer
According to the rice varieties Japan providing in NCBI fine (Oryza sativa L cv.Nipponbare) whole genome sequence, according to the sequences Design amplimer of paddy rice PCole1 gene, and according to the feature of the carrier of selecting and target gene, the restriction enzyme site of design primer.
In the present embodiment with paddy rice binary expression vector pCAMBIA1391(Figure 1A, come from CAMBIA, openly use carrier, genetically modified organism product composition supervision and inspection center of Academy of Agri-Science and Technology Anhui Province Ministry of Agriculture paddy rice group is preserved) be example, target gene is Gus gene, the primer of specific design is: forward primer (SEQ ID No:2) 5 ' end band SalI, restriction enzyme site (GTCGAC), reverse primer (SEQ ID No:3) 5 ' end band SmaI, restriction enzyme site (CCCGGG), primer sequence is as follows:
Forward primer: GTCGACTCCAGTTGATTACCATCAGATG SalI
Reverse primer: CCCGGGCTCCAGACTTGAATCGAAGATT SmaI
Synthetic by Shenzhen Hua Da genome company.
The acquisition of step 2, promotor Poscold2
Take the fine DNA of rice varieties Japan as template, utilize forward primer, reverse primer amplification promotor Poscold2, PCR system routinely, adopts following amplification program:
95 ℃ of denaturation 5min; 95 ℃ of sex change 30s, 58 ℃ of annealing 30s, 72 ℃ are extended 2min30s, carry out 35 circulations from 95 ℃ of denaturation to 72 ℃ extensions; Last 72 ℃ are extended 10min.
Reclaim the object fragment of pcr amplification, object fragment length 1961bp, be connected to PGEM-T-Easy carrier (purchased from Promega company, mix in the ratio in carrier specification sheets) on, transform after intestinal bacteria XL-Blue competent cell according to heat shock method, competent cell is activated, and then object fragment is transferred in the competent cell of activation, then, obtain positive colony through bacterium colony PCR screening, picking mono-clonal shakes bacterium liquid upgrading grain, carries out double digestion checking with SalI and SmaI, as shown in Figure 3.The order-checking of Invitrogen company will be delivered through the positive colony of identifying.Verify that correct clone is the promotor Poscold2 that will obtain, its nucleotide sequence is as shown in SEQ ID No:1.
The structure of plant expression vector and the conversion of Agrobacterium
In the positive colony obtaining " acquisition of promotor Poscold2 " process from above, extract plasmid, with SalI and SmaI double digestion, reclaim promotor Poscold2 fragment.Utilize SalI and SmaI to carry out linearization process to pCAMBIA1391 simultaneously, reclaim pCAMBIA1391, above-mentioned Poscold2 fragment is connected with T4 ligase enzyme for pCAMBIA1391 fragment (being purchased from TaKaRa company), obtain plant expression vector pCAMBIA1391-Poscold2(Figure 1B of promotor Poscold2 and Gus gene fusion), utilizing freeze-thaw method that plant expression vector is proceeded to agrobacterium tumefaciens (Agrobacterium tumefaciens) EHA105(Academy of Agri-Science and Technology Anhui Province genetically modified organism product composition supervision and inspection center of Ministry of Agriculture paddy rice group preserves), from freeze-thaw method products therefrom, extract positive plasmid, carry out enzyme with SalI and SmaI and cut checking, the result as shown in Figure 3.
Utilize promotor Poscold2 to drive Gus reporter gene to express in paddy rice
Step 1: agriculture bacillus mediated rice transformation
Ripe rice paddy seed is removed after clever shell, with 70% alcohol-pickled seed 1min, outwell alcohol.With 50% clorox that contains 1 Tween20 (stoste effective chlorine density is greater than 4%) solution soaking seed 40min(150r/min).Outwell clorox, aseptic washing 5 times is to solution clarification, without clorox taste.Sterilized water soaks seed and spends the night.Seed is peeled embryo along aleurone layer with scalper, embryo is inoculated on calli induction media.Dark cultivation after 11 days callus and endosperm and germ separation at 30 ℃, by go bud in good condition, divide vigorous elementary callus carry out preculture after 3～5 days for Agrobacterium-mediated Transformation.
Adopt the agrobacterium tumefaciens that has proceeded to recombinant expression vector in above-mentioned " structure of plant expression vector and the conversion of Agrobacterium " process to carry out agriculture bacillus mediated genetic transformation, this genetic transformation, transformant screening and transgenic plant regeneration etc. are with reference to Yongbo Duan(Yongbo Duan, Chenguang Zhai, et al.An efficient and high-throughput protocol for Agrobacterium mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.) [J] .Plant Cell Report, method 2012.DOI10.1007/s00299-012-1275-3.) etc. proposing.
Obtain altogether 22 strain pCAMBIA1391-Poscold2 plant (Poscold2::gus transgenic rice plant).
Step 2, deepfreeze and GUS histochemical stain
By the deepfreeze after 24 hours at 4 ℃ of obtained plant, again through 30minGUS dyeing, dyeing process with reference to Jefferson (the people .GUS fusion such as Jefferson RA: β-Glucuronidase as a sensitive and versatile gene fusion marker in higher plant[J] .EMBO J., 1987, method 6:3901-3907) etc. proposing, the tissue of needs dyeing is vacuumized, then immerse in staining fluid.When decolouring, under 37 ℃ of conditions, use 95% Ethanol Treatment, extremely negative control material is white in color.
The Poscold2::GUS transfer-gen plant tissue of sprouting after 21 days is dyeed.As shown in Figure 2, the rice plant of normal growth under 28 ℃ of conditions, after dyeing in 24 hours, root (A), stem (B), leaf (C) are all without GUS activity, and 4 ℃ of deepfreezes after 24 hours, then after 30min dyeing, in root (D), stem (E), leaf (F), all there is GUS strong expression.
Specific description of embodiments of the present invention above does not limit the present invention, and those skilled in the art can make according to the present invention various changes or distortion, only otherwise depart from spirit of the present invention, all should belong to the scope of claims of the present invention.
Claims (9)
1. the cold abduction delivering promotor of kind of plant Poscold2, is characterized in that, the cold abduction delivering promotor of described plant Poscold2 comprises the DNA sequence dna shown in SEQ ID No:1.
2. the cold abduction delivering promotor of plant according to claim 1 Poscold2, is characterized in that, the DNA sequence dna of the cold abduction delivering promotor of described plant Poscold2 is the sequence shown in SEQ ID No:1.
3. the cold abduction delivering promotor of plant according to claim 1 Poscold2, is characterized in that, the DNA sequence dna shown in DNA sequence dna and the SEQ ID No:1 of the cold abduction delivering promotor of described plant Poscold2 has at least 80% homology;
Or the cold abduction delivering promotor of described plant Poscold2 add, replace, insert or delete mutant or allelotrope or the derivative that one or more Nucleotide generate in the DNA sequence dna shown in SEQ ID No:1;
Or the cold abduction delivering promotor of described plant Poscold2 has the product with the DNA sequence dna hybridization shown in SEQ ID No:1.
4. an expression cassette, is characterized in that, described expression cassette comprises the cold abduction delivering promotor of the plant described in any one Poscold2 in claim 1-3.
5. a recombinant expression vector, it is characterized in that, described recombinant expression vector comprises the cold abduction delivering promotor of the plant described in any one Poscold2 in claim 1-3, in described recombinant expression vector, the cold abduction delivering promotor of described plant Poscold2 is connected in the upstream of gene order to be expressed in carrier;
Preferably, described gene to be expressed is Gus gene, and described recombinant expression vector is pCAMBIA1391-Poscold2, and wherein pCAMBIA1391 is plant binary expression vector, or described gene to be expressed is the gene with resistance to cold.
6. a Host Strains, it is characterized in that, described Host Strains comprises the cold abduction delivering promotor of the plant described in any one Poscold2, expression cassette claimed in claim 4 or recombinant expression vector according to claim 5 in claim 1-3, wherein, described Host Strains is agrobacterium tumefaciens.
7. a transformant, is characterized in that, described transformant comprises the cold abduction delivering promotor of the plant described in any one Poscold2, expression cassette claimed in claim 4 or recombinant expression vector according to claim 5 in claim 1-3.
8. the application in cultivation transgenic plant according to the cold abduction delivering promotor of the plant Poscold2 described in any one in claim 1-3, it is characterized in that, described application comprises: will be connected in gene order upstream to be expressed in carrier according to the cold abduction delivering promotor of the plant Poscold2 described in any one in claim 1-3, thereby build recombinant expression vector; Described recombinant expression vector is transformed in vegetable cell, tissue or organ and is cultivated.
9. application according to claim 8, is characterized in that, described application is for improving plant growth characteristic, and described plant is monocotyledons: paddy rice, wheat, corn, barley, Chinese sorghum or oat.
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| CN104059919A (en) * | 2014-07-08 | 2014-09-24 | 安徽省农业科学院水稻研究所 | Plant thermal-activation inducible promoter Posheat3 and application thereof |
| CN104073493A (en) * | 2014-07-09 | 2014-10-01 | 安徽省农业科学院水稻研究所 | Cold-induced and strong-expressed plant promoter Poscold4 and application thereof |
| CN104073493B (en) * | 2014-07-09 | 2016-06-22 | 安徽省农业科学院水稻研究所 | The cold induction strongly expressed promoter Poscold4 of plant and application thereof |
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| CN105063053A (en) * | 2015-09-23 | 2015-11-18 | 安徽省农业科学院水稻研究所 | Plant cold-induced expression promoter POsCold9 and derivative thereof |
| CN105087590A (en) * | 2015-09-23 | 2015-11-25 | 安徽省农业科学院水稻研究所 | Promoter element POsCold8 and using method and application thereof |
| CN105087590B (en) * | 2015-09-23 | 2017-10-20 | 安徽省农业科学院水稻研究所 | A kind of promoter element POsCold8 and its application method and application |
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| CN103849622B (en) | 2016-01-27 |
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