CN103843974A - Compound microecologics and application thereof - Google Patents

Abstract

The invention discloses a composite micro-ecological preparation, which is composed of raw materials of Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus plantarum in parts by weight of 1:1:1. The present invention optimizes the combination of animal probiotics and screens an optimized combination of probiotics that can effectively reduce the content of nitrogen and phosphorus in the manure of nursery pigs without reducing the growth performance. Provide scientific basis for future environmental pollution. The experimental results show that the compound microecological preparation can effectively improve the apparent digestibility of nitrogen and phosphorus, reduce the content of nitrogen and phosphorus in manure, and reduce the pollution of livestock and poultry manure to the environment.

Description

A kind of compound probiotics and its application

technical field

The invention belongs to the technical field of application of compound microecological preparations, and relates to the research on the influence of different combinations of probiotics on the reduction of nitrogen and phosphorus emissions from nursery pig manure, and more specifically relates to a compound microecological preparation and its application.

Background technique

In recent years, with the rapid development of large-scale livestock and poultry breeding industry, the problem of environmental pollution has become increasingly apparent, and has attracted widespread attention from the society. Reducing the environmental pollution caused by livestock and poultry farming and developing an eco-friendly animal husbandry economy have become the key factors to maintain the sustainable development of animal husbandry. Zhang Yongyong (2008) added compound probiotics composed of spores, lactic acid bacteria, and bifidobacteria to the diet of weaned piglets, and found that adding 0.05% of compound probiotics can significantly improve the daily feed intake and crude protein digestion of piglets. rate and serum immunoglobulin levels. Ning Yuchang (2009) and other studies have shown that adding 8% probiotics to the basic diet of weaned piglets, the crude protein digestibility of the probiotics group increased by 7.32% and 11.47% compared with the control group, the difference was Statistically significant (P<0.05). At present, many studies focus on the digestibility of crude protein and crude fiber, but the research on the impact of nitrogen and phosphorus discharge on the environment has not yet been carried out. The present invention aims to select an optimized probiotic combination that can effectively reduce the nitrogen and phosphorus content in nursery pig manure by optimizing the combination of animal probiotics without reducing the growth performance. Provide scientific basis for environmental pollution caused by industry.

Contents of the invention

The invention discloses a compound micro-ecological preparation, which is characterized in that it is composed of raw materials of Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus plantarum in parts by weight of 1:1:1. Among them, the dosage of Bacillus subtilis is 0.2-0.9% (w/w), the dosage of Saccharomyces cerevisiae is 0.2-0.9% (w/w), and the dosage of Lactobacillus plantarum is 0.2-0.9% (w/w).

The viable count of Bacillus subtilis in the present invention is (1.0×10 ¹⁰ cfu/g),

The viable count of Saccharomyces cerevisiae is (1.0×10 ¹⁰ cfu/g),

The viable count of Lactobacillus plantarum was (1.0×10 ¹⁰ cfu/g).

The invention further discloses the application of the compound microecological preparation in reducing environmental pollution caused by livestock and poultry manure. Especially in the application of reducing the nitrogen and phosphorus content of nursery pig manure. Reducing the nitrogen and phosphorus content of nursery pig manure in the present invention refers to effectively improving the apparent digestibility of nitrogen and phosphorus, and reducing the nitrogen and phosphorus content in manure.

Fermentation production method of Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus plantarum (according to the medium formula and culture conditions provided by the strain collection center, it is enough to cultivate to the required number of viable bacteria).

The more detailed test and experimental results of the present invention are as follows:

1 Materials and methods

1.1 Test material

The source of Bacillus subtilis used in this test:

Bacillus subtilis ACCC11025, dosage 0.2-0.9%;

Saccharomyces cerevisiae CICC1421, dosage 0.2-0.9%;

Lactobacillus plantarum ACCC11118, dosage 0.2-0.9%;

The above-mentioned probiotics are commercially available and then preserved in the laboratory.

1.2 Experimental animals and experimental design:

The experiment adopts a single factor experimental design, selects 16 nursery pigs of similar age and body weight of 35kg (± 2kg), and randomly divides them into four treatment groups, with 4 repetitions in each treatment group. The first group of control group was fed with basal diet, the second group of treatment group 1 added 0.5% live bacteria of Bacillus subtilis (1.0×10 ¹⁰ cfu/g) on the basis of the basal diet, and the third group of treatment group 2 Add 0.5% of Bacillus subtilis and 0.5% of Saccharomyces cerevisiae live bacteria (1.0×10 ¹⁰ cfu/g) on the basis of the basic diet, and add 0.5% of the fourth treatment group 3 on the basis of the basic diet Bacillus subtilis, 0.5% Saccharomyces cerevisiae and 0.5% Lactobacillus plantarum live bacteria (1.0×10 ¹⁰ cfu/g). The composition and nutrient levels of the basal diet are shown in Table 1.

Table 1 Composition and nutritional level of basal diet

Diet Composition content% nutritional level content% corn 62 Digestible energy (MJ/kg) 14 soybean meal twenty two protein(%) 19 Compound premix 16 calcium(%) 0.7 the the Total phosphorus (%) 0.65 the the salt(%) 0.3 the the Lysine (%) 1.35 the the Egg + Cystine (%) 0.8 the the Threonine(%) 0.87

1.3 Feeding management

Thoroughly clean and rinse the stalls and feed utensils before the test, and have free access to food and water during the pre-test period. After the 3d pre-test period, the test pigs were starved for 24 hours on an empty stomach, and entered into the formal test. The experimental pigs were reared in separate pens, fed with powder, and fed regularly and quantitatively. The rest of the feed was collected the next morning, and they drank water freely. The main test period is 1 day, and the feces of each test pig are collected independently within 24 hours of the day, and the defecation and physical condition of the 16 pigs are observed in time.

1.4 Index determination

1.4.1 Indirect method to measure apparent digestibility of nitrogen and phosphorus Apparent digestibility was measured by acid-insoluble ash (AIA) indirect method (Cui Baohu, 1981; Xu Fengxia, 2004), that is, endogenous indicator method. Calculated as follows:

Apparent digestibility of a nutrient (%)=100×[1-(A/B)×(C/D)]

Where A is the nutrient content in feces, B is the nutrient content in feed, C is the acid-insoluble ash (AIA) content in feed, and D is the acid-insoluble ash content in feces.

1.4.2 Apparent digestibility of nitrogen and phosphorus in total collection of feces (Zhang Zhongyuan, 2004) The calculation formula is as follows:

Apparent digestibility of a nutrient (%)=100×[(A-B)/A]

A is the nutrient ingested, and B is the nutrient in the feces.

1.5 Statistical analysis of data The experimental data was analyzed using SPSS19.0 statistical software, and One-way ANOVA was used for analysis of variance, LSD method combined with Duncan's method for multiple comparisons between groups, and the results were expressed as "mean ± standard deviation".

2 Results and analysis

2.1 Effects of different probiotics on feed intake and weight gain of nursery pigs

It can be seen from Table 2 that the addition of different probiotics did not significantly change the feed intake, and the difference was not significant (P>0.05); the total weight gain among the experimental groups did not change much, and the difference was not significant (P>0.05).

Table 2 Effects of different probiotics on feed intake and weight gain of nursery pigs

the control group treatment group 1 treatment group 2 treatment group 3 feed intake 1671.25±68.27 ^a 1682.00±13.74 ^a 1651.00±50.98 ^a 1673.75±69.87 ^a Initial gross weight (kg) 56.75±3.77 ^a 58.25±3.30 ^a 57.75±1.71 ^a 56.50±1.91 ^a Final gross weight (kg) 62.75±3.40 ^a 65.75±3.86 ^a 61.50±3.00 ^a 63.00±6.38 ^a Total weight gain (kg) 6.00±1.41 ^a 7.5±2.89 ^a 3.75±2.75 ^a 6.50± ^5.07a

Note: The different lowercase letters on the shoulders of peer data indicate significant differences ( P < 0. 05 )

2.2 Effects of different probiotics on the apparent digestibility of nitrogen in nursery pigs

It can be seen from Table 3 that the ingested nitrogen content was significantly different (P<0.05) among the control group, treatment group 1, treatment group 2, and treatment group 3 due to the difference in the added probiotics and feed intake (P<0.05); The nitrogen content tended to decrease with the change of ingested nitrogen level, and the treatment group 3 was the lowest, and the difference was extremely significant (P<0.01); the apparent digestibility of nitrogen in treatment group 2 and treatment group 3 measured by AIA indirect method was higher than that of the control group group increased by 58.65%, 39.00%, the difference was extremely significant (P<0.01), the difference between treatment group 1 and the control group was not significant (P>0.05); The nitrogen apparent digestibility of group 3 increased by 18.16%, 8.89%, and 33.14% compared with the control group, and there was no significant difference between treatment group 1 and treatment group 2 and the control group. The difference between treatment group 3 and the control group was extremely significant (P<0.01).

Table 3 Effects of different probiotics on the apparent digestibility of nitrogen in nursery pigs

the control group treatment group 1 treatment group 2 treatment group 3 Ingested N content (g/d) 29.25± ^1.19ab 29.27± ^0.24ab 30.54±0.94 ^a 28.62± ^1.19b Manure production (g) 375.25±91.54 352.75±81.46 408.50±33.15 311.25±60.66 N content in feces (g/d) 16.37±2.55 ^Aa 14.01±2.64 ^{A Abab} 15.86±0.34 ^ABa 11.80±0.88 ^Bb AIA indirect method N apparent digestibility (%) 32.82±1.28 ^Bb 30.66±7.39 ^Bb 52.07±4.75 ^Aa 45.62±3.98 ^Aa Apparent digestibility of N in total harvested manure method (%) 44.11±7.33 ^Bb 52.12± ^9.00ABab 48.03± ^1.71ABb 58.73±3.46 ^Aa

Note: different lowercase letters in the shoulders of peer data indicate significant differences ( P < 0. 05 ); different uppercase letters indicate extremely significant differences ( P < 0. 01 ); no letters indicate insignificant differences ( P > 0. 05 ), below The table is the same.

2.3 Effects of different microecological preparations on the apparent digestibility of phosphorus in nursery pigs

It can be seen from Table 4 that due to the difference in the added probiotics and feed intake, there were significant differences (P<0.05) between the control group and treatment group 3, treatment group 1, and treatment group 2 (P<0.05); Phosphorus content in the control group, treatment group 1 and treatment group 2 was relatively close, while treatment group 3 was the lowest, but the difference between the test groups was not significant (P> 0.05); AIA indirect method measured the treatment group 2, treatment group Compared with the control group, the apparent digestibility of phosphorus in 3 increased by 93.80% and 71.46%, the difference was extremely significant (P<0.01), and the difference between treatment group 1 and the control group was not significant (P > 0.05); The apparent digestibility of phosphorus in treatment group 1, treatment group 2, and treatment group 3 increased by 24.93%, 6.57%, and 48.99% compared with the control group, and the differences between treatment group 1, treatment group 3 and the control group were extremely significant (P <0.01).

Table 4 Effects of different microecological preparations on the apparent digestibility of phosphorus in nursery pigs

the control group treatment group 1 treatment group 2 treatment group 3 Ingested P content (g/d) 10.86±0.44 ^Bc 12.62±0.10 ^Aa 11.56±0.36 ^Bb 10.88±0.45 ^Bc Manure production (g) 375.25±91.54 352.75±81.46 408.50±33.15 311.25±60.66 P content in feces (g/d) 7.04±0.60 7.07±0.65 7.25±0.29 5.18±0.46 AIA indirect method P apparent digestibility (%) 21.62±4.70 ^Bb 18.07±6.43 ^Bb 41.90±8.58 ^Aa 37.07±7.09 ^Aa Apparent digestibility of P in the whole collection method (%) 35.17± ^4.65C 43.94± ^4.82B 37.30± ^1.11BC 52.40± ^3.61A

3 Discussion

The results of this test show that the AIA indirect method measured the apparent digestibility of the treatment group 2 and the treatment group 3 compared with the control group and the treatment group 1, and the effect of the treatment group 2 was the most obvious, and the apparent digestibility of nitrogen increased by 58.65%. %, the apparent digestibility of phosphorus increased by 93.80%, but the difference between treatment group 2 and treatment group 3 was not significant; the effect of treatment group 3 was the most obvious in the determination of the apparent digestibility of the total feces method, which can effectively improve the nitrogen table The apparent digestibility of nitrogen was 33.14% and the apparent digestibility of phosphorus was 48.99%, which were significantly different from other groups, but there was no significant difference between the treatment group 2 and the control group. Significant differences.

4 Summary

The experimental results show that the treatment group 3, that is, the compound probiotics is composed of 1:1:1, especially Bacillus subtilis + Lactobacillus plantarum + Saccharomyces cerevisiae, the dosage range of each component is 0.2-0.9%, and the effect is the best at this time. good. The compound microecological preparation can effectively improve the apparent digestibility of nitrogen and phosphorus, reduce the content of nitrogen and phosphorus in feces, and reduce the pollution of livestock and poultry manure to the environment.

the

Detailed ways

The present invention is illustrated below in conjunction with the examples, the scheme of the examples described here does not limit the present invention, and those skilled in the art can improve and change it according to the spirit of the present invention, and these described improvements and changes should be regarded as Within the scope of the present invention, the scope and spirit of the present invention are defined by the claims. Wherein Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are all commercially available.

Example 1

Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus plantarum are composed of raw materials in parts by weight of 1:1:1. Among them, the viable count of Bacillus subtilis (1.0×10 ¹⁰ cfu/g), the viable count of Saccharomyces cerevisiae (1.0×10 ¹⁰ cfu/g), the viable count of Lactobacillus plantarum (1.0×10 ¹⁰ cfu/g) g).

The dosage of Bacillus subtilis is 0.2% (w/w), the dosage of Saccharomyces cerevisiae is 0.2% (w/w), and the dosage of Lactobacillus plantarum is 0.2% (w/w).

Example 2

Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus plantarum are composed of raw materials in parts by weight of 1:1:1. Among them, the viable count of Bacillus subtilis (1.0×10 ¹⁰ cfu/g), the viable count of Saccharomyces cerevisiae (1.0×10 ¹⁰ cfu/g), the viable count of Lactobacillus plantarum (1.0×10 ¹⁰ cfu/g) g).

The dosage of Bacillus subtilis is 0.5% (w/w), the dosage of Saccharomyces cerevisiae is 0.5% (w/w), and the dosage of Lactobacillus plantarum is 0.5% (w/w).

Example 3

Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus plantarum are composed of raw materials in parts by weight of 1:1:1. Among them, the number of live bacteria of Bacillus subtilis (1.0×10 ¹⁰ cfu/g), the number of viable bacteria of Bacillus subtilis (1.0×10 ¹⁰ cfu/g), and the number of viable bacteria of Lactobacillus plantarum (1.0×10 ¹⁰ cfu/g) .

The dosage of Bacillus subtilis is 0.9% (w/w), the dosage of Saccharomyces cerevisiae is 0.9% (w/w), and the dosage of Lactobacillus plantarum is 0.9% (w/w).

Claims (5)

1. a compound micro-ecological preparation, it is characterized in that it by bacillus subtilis, S. cervisiae, Lactobacillus plantarum by weight the raw material of 1:1:1 form, wherein said bacillus subtilis viable count (1.0 × 10 ¹⁰cfu/g), S. cervisiae viable count is (1.0 × 10 ¹⁰cfu/g), Lactobacillus plantarum viable count is (1.0 × 10 ¹⁰cfu/g).

2. compound micro-ecological preparation claimed in claim 1, wherein bacillus subtilis consumption 0.2-0.9% (w/w), S. cervisiae consumption 0.2-0.9% (w/w), Lactobacillus plantarum consumption 0.2-0.9% (w/w).

3. the application of the compound micro-ecological preparation described in claim 1 or 2 aspect reduction feces of livestock and poultry environmental pollution.

4. the application of the compound micro-ecological preparation described in claim 1 or 2 aspect the dirty the content of nitrogen and phosphorous of reduction child care pig manure.

5. application claimed in claim 4, the dirty the content of nitrogen and phosphorous of wherein said reduction child care pig manure refers to: effectively improve the apparent digestibility of nitrogen, phosphorus, reduce the content of nitrogen and phosphorous in ight soil.