CN103843974A - Compound microecologics and application thereof - Google Patents
Compound microecologics and application thereof Download PDFInfo
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- CN103843974A CN103843974A CN201410112422.8A CN201410112422A CN103843974A CN 103843974 A CN103843974 A CN 103843974A CN 201410112422 A CN201410112422 A CN 201410112422A CN 103843974 A CN103843974 A CN 103843974A
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- nitrogen
- phosphorous
- bacillus subtilis
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- lactobacillus plantarum
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Abstract
The invention discloses a compound microecologics containing raw materials such as bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum according to part by weight of 1:1:1. According to the compound microecologics, combinatorial optimization is carried out on animal probiotics, and an optimized probiotics combination capable of effectively lowering the contents of nitrogen and phosphorous in nursed pig manure is screened out, a scientific basis is provided for effectively alleviating environmental pollution caused by livestock and poultry industry. An experimental result shows that the compound microecologics can effectively improve the apparent digestibility of nitrogen and phosphorous, reduce the contents of nitrogen and phosphorous in the manure, and alleviate the pollution of the poultry excrement to the environment.
Description
Technical field
The invention belongs to compound micro-ecological preparation applied technical field, relate to the impact research that different probiotic combinations reduces discharging the dirty nitrogen of child care pig manure, phosphorus, say more specifically a kind of compound micro-ecological preparation and application thereof.
Background technology
In recent years, along with the high speed development of scale animal and poultry cultivation industry, cause problem of environmental pollution day by day to manifest, and be subject to social extensive concern.Reduce the environmental pollution that livestock and poultry breeding industry brings, developing ecology environment-friendly type livestock economy, has become the key factor that keeps sustainable development of animal husbandry.Zhang Yongyong (2008) etc. add the composite prebiotics of the compositions such as Bacillus, lactic acid bacteria, Bifidobacterium in weanling pig daily ration, found that the composite prebiotics that adds 0.05%, can significantly improve daily ingestion amount, crude protein digestibility and the serum immunoglobulin level of piglet.The researchs such as Ning Yuchang (2009) show, in weanling pig basal diet, add 8% probiotics, add probiotics group crude protein digestibility compared with control group and improved respectively 7.32% and 11.47%, difference has statistical significance (P<0.05).Current more research focuses mostly in the research of crude protein and crude fiber digestibility aspect, and nitrogen, phosphorus discharge are not yet carried out the research of environment aspect impact.The present invention is intended to by animal probio is carried out to Combinatorial Optimization, do not reducing under the prerequisite of growth performance, screen a kind of optimization probiotic combinations that can effectively reduce the content of nitrogen and phosphorous in child care swine excrement, provide scientific basis for utilizing probio effectively to reduce the environmental pollution that poultry industry brings.
Summary of the invention
The invention discloses a kind of compound micro-ecological preparation, it is characterized in that it by bacillus subtilis, S. cervisiae, Lactobacillus plantarum by weight the raw material of 1:1:1 form.Wherein bacillus subtilis consumption is 0.2-0.9% (w/w), S. cervisiae consumption 0.2-0.9% (w/w), Lactobacillus plantarum consumption 0.2-0.9% (w/w).
Bacillus subtilis viable count of the present invention is (1.0 × 10
10cfu/g),
S. cervisiae viable count is (1.0 × 10
10cfu/g),
Lactobacillus plantarum viable count is (1.0 × 10
10cfu/g).
The present invention further discloses the application of compound micro-ecological preparation aspect reduction feces of livestock and poultry environmental pollution.The particularly application aspect the dirty the content of nitrogen and phosphorous of reduction child care pig manure.The dirty nitrogen and phosphorus content of reduction child care pig manure of the present invention refers to the apparent digestibility of effective raising nitrogen, phosphorus, reduces the content of nitrogen and phosphorous in ight soil.
Bacillus subtilis, S. cervisiae, Lactobacillus plantarum fermentation method for producing (culture medium prescription providing by DSMZ and condition of culture are cultivated required viable count).
The more detailed test of the present invention and experimental result are as follows:
1 materials and methods
1.1 test material
The bacillus subtilis source that this test is used:
Bacillus subtilis ACCC11025, consumption 0.2-0.9%;
S. cervisiae CICC1421, consumption 0.2-0.9%;
Lactobacillus plantarum ACCC11118, consumption 0.2-0.9%;
Above-mentioned probio all has commercially available, then preserves in laboratory.
1.2 experimental animals and experimental design:
Test adopts single factor experiment design, chooses age in days and approaches, and 16 of the child care pigs of body weight 35kg (± 2kg), are divided into four processed group at random, 4 repetitions of each processed group.First group of control group fed basal diet, second group of processed group 1 added 0.5% bacillus subtilis viable count (1.0 × 10 on the basis of basal diet
10cfu/g), the 3rd group of processed group 2 added 0.5% bacillus subtilis and 0.5% S. cervisiae viable count (1.0 × 10 on the basis of basal diet
10cfu/g), the 4th group of processed group 3 added 0.5% bacillus subtilis, 0.5% S. cervisiae and 0.5% Lactobacillus plantarum viable count (1.0 × 10 on the basis of basal diet
10cfu/g).Basal diet composition and trophic level are in table 1.
Table 1 basal diet composition and trophic level
| Daily ration composition | Content % | Trophic level | Content % |
| Corn | 62 | Digestible energy (MJ/kg) | 14 |
| Dregs of beans | 22 | Protein (%) | 19 |
| Compound premix | 16 | Calcium (%) | 0.7 |
| ? | ? | Total phosphorus (%) | 0.65 |
| ? | ? | Salt (%) | 0.3 |
| ? | ? | Lysine (%) | 1.35 |
| ? | ? | Egg+cystine (%) | 0.8 |
| ? | ? | Threonine (%) | 0.87 |
1.3 feeding and management
Before test, thoroughly column home, feed apparatus, free choice feeding and drinking-water during preliminary trial period are rinsed in cleaning.After 3d preliminary trial period, test pig is carried out to hunger and within 24 hours, process on an empty stomach, enter formal test.Test pig adopts subfield to raise, and powder is fed, and timing site-directed quantitative feeding, turns and reclaim residual feed a day morning, freely drinks water.Just the examination phase is 1d, and every test pig was discharged feces and independently collected respectively within 24 hours same day, with drawing with receipts, and defecation and the health of observing in time 16 pigs.
1.4 index determining
1.4.1 indirect method is surveyed mensuration employing acid insoluble ash (AIA) indirect method (Cui Baohu, 1981 of nitrogen, phosphorus apparent digestibility apparent digestibility; Xu Fengxia, 2004), i.e. endogenous indicator method.Computing formula is as follows:
Certain Nutrient Apparent Digestibility (%)=100 × [1-(A/B) × (C/D)]
Wherein A is this nutrient content in excrement, and B is this nutrient content in feed, and C is acid insoluble ash (AIA) content in feed, and D is acid insoluble ash content in excrement.
1.4.2 entirely receive excrement method nitrogen, phosphorus apparent digestibility (Zhang Zhongyuan, 2004) computing formula is as follows:
Certain Nutrient Apparent Digestibility (%)=100 × [(A-B)/A]
Middle A is for eating this nutrient, and B is this nutrient in excrement.
1.5 data statistic analysis test datas adopt SPSS19.0 statistical software to carry out statistical analysis, adopt One-way ANOVA to carry out variance analysis, LSD method organize in conjunction with Duncan ' s method between multiple ratio, result all represents with " average ± standard deviation ".
2 results and analysis
2.1 impacts of different probioticses on child care pig feed intake and weightening finish
As shown in Table 2, add different probioticses feed intake is not significantly changed, difference is remarkable (P> 0.05) not; Between each experimental group, total augment weight changes not quite, difference not obvious (P > 0.05).
The impact of the different probioticses of table 2 on child care pig feed intake and weightening finish
| ? | Control group | Processed group 1 | Processed group 2 | Processed group 3 |
| Feed intake | 1671.25±68.27 a | 1682.00±13.74 a | 1651.00±50.98 a | 1673.75±69.87 a |
| Initial gross weight (kg) | 56.75±3.77 a | 58.25±3.30 a | 57.75±1.71 a | 56.50±1.91 a |
| End gross weight (kg) | 62.75±3.40 a | 65.75±3.86 a | 61.50±3.00 a | 63.00±6.38 a |
| Total augment weight (kg) | 6.00±1.41 a | 7.5±2.89 a | 3.75±2.75 a | 6.50±5.07 a |
Note: the different lowercase alphabets of colleague's data shoulder motes show significant difference (P < 0. 05)
The impact of 2.2 different probioticses on child care pig nitrogen apparent digestibility
As shown in Table 3, eat nitrogen content due to the probiotics adding and the difference of feed intake, make that between control group, processed group 1 and processed group 2, processed group 3, there were significant differences (P<0.05); In ight soil, nitrogen content has the trend of reduction along with eating the variation of nitrogen level, minimum with processed group 3, and difference is (P<0.01) extremely significantly; The nitrogen apparent digestibility that AIA indirect method records processed group 2, processed group 3 has improved 58.65%, 39.00% than control group, difference is (P<0.01) extremely significantly, not remarkable (P > 0.05) of difference between processed group 1 and control group; The full excrement method of receiving records between the nitrogen apparent digestibility of processed group 1, processed group 2, processed group 3 and control group and has improved 18.16%, 8.89%, 33.14%, and between processed group 1 and processed group 2 and control group, difference is not remarkable.And extremely significantly (P<0.01) of difference between processed group 3 and control group.
The impact of the different probioticses of table 3 on child care pig nitrogen apparent digestibility
| ? | Control group | Processed group 1 | Processed group 2 | Processed group 3 |
| Eat N content (g/d) | 29.25±1.19 ab | 29.27±0.24 ab | 30.54±0.94 a | 28.62±1.19 b |
| Produce excrement amount (g) | 375.25±91.54 | 352.75±81.46 | 408.50±33.15 | 311.25±60.66 |
| N content (g/d) in excrement | 16.37±2.55 Aa | 14.01±2.64 ABab | 15.86±0.34 ABa | 11.80±0.88 Bb |
| AIA indirect method N apparent digestibility (%) | 32.82±1.28 Bb | 30.66±7.39 Bb | 52.07±4.75 Aa | 45.62±3.98 Aa |
| The complete apparent digestibility (%) of receiving excrement method N | 44.11±7.33 Bb | 52.12±9.00 ABab | 48.03±1.71 ABb | 58.73±3.46 Aa |
Note: the different lowercase alphabets of colleague's data shoulder motes show significant difference (P < 0. 05); Different capitalizations represent extremely significantly (P < 0. 01) of difference; Without not remarkable (P > 0. 05) of letter representation difference, following table is same.
The impact of 2.3 different probioticses on child care pig phosphorus apparent digestibility
As shown in Table 4, eat phosphorus content due to the probiotics adding and the difference of feed intake, make that between control group and processed group 3 and processed group 1, processed group 2, there were significant differences (P<0.05); In ight soil, phosphorus content is at control group, more approaching between processed group 1 and processed group 2, and processed group 3 is minimum, but not remarkable (P> 0.05) of difference between test group; The phosphorus apparent digestibility that AIA indirect method records processed group 2, processed group 3 has improved 93.80%, 71.46% than control group, difference is (P<0.01) extremely significantly, not remarkable (P > 0.05) of difference between processed group 1 and control group; In full receipts excrement method, between the phosphorus apparent digestibility of processed group 1, processed group 2, processed group 3 and control group, improve 24.93%, 6.57%, 48.99%, extremely significantly (P<0.01) of difference between processed group 1 and processed group 3 and control group.
The impact of the different probioticses of table 4 on child care pig phosphorus apparent digestibility
| ? | Control group | Processed group 1 | Processed group 2 | Processed group 3 |
| Eat P content (g/d) | 10.86±0.44 Bc | 12.62±0.10 Aa | 11.56±0.36 Bb | 10.88±0.45 Bc |
| Produce excrement amount (g) | 375.25±91.54 | 352.75±81.46 | 408.50±33.15 | 311.25±60.66 |
| P content (g/d) in excrement | 7.04±0.60 | 7.07±0.65 | 7.25±0.29 | 5.18±0.46 |
| AIA indirect method P apparent digestibility (%) | 21.62±4.70 Bb | 18.07±6.43 Bb | 41.90±8.58 Aa | 37.07±7.09 Aa |
| The complete apparent digestibility (%) of receiving excrement method P | 35.17±4.65 C | 43.94±4.82 B | 37.30±1.11 BC | 52.40±3.61 A |
3 discuss
This result of the test shows, in the every apparent digestibility of AIA indirect Determination, processed group 2 and processed group 3 all improve with respect to control group and processed group 1, wherein processed group 2 effects are the most obvious, nitrogen apparent digestibility has improved 58.65%, phosphorus apparent digestibility has improved 93.80%, but between processed group 2 and processed group 3, difference is not remarkable; Complete receive excrement method to measure in every apparent digestibility processed group 3 effects the most obvious, can effectively improve nitrogen apparent digestibility 33.14% and phosphorus apparent digestibility 48.99%, all extremely remarkable with other group differences, and difference is not remarkable between processed group 2 and control group, between the nitrogen apparent digestibility of processed group 1 and control group, there is significant difference.
4 brief summaries
Experimental result shows, processed group 3 is that compound micro-ecological preparation forms by 1:1:1, particularly bacillus subtilis+Lactobacillus plantarum+saccharomyces cerevisiae, and the amount ranges of each composition is 0.2-0.9%, now best results.Compound micro-ecological preparation can effectively improve the apparent digestibility of nitrogen, phosphorus, reduces the content of nitrogen and phosphorous in ight soil, reduces the pollution of feces of livestock and poultry to environment.
The specific embodiment
Below in conjunction with embodiment, the present invention is described, the scheme of embodiment described here, do not limit the present invention, one of skill in the art can make improvements and change according to spirit of the present invention, these described improvement and variation all should be considered as within the scope of the invention, and scope of the present invention and essence are limited by claim.Wherein bacillus subtilis, Lactobacillus plantarum, saccharomyces cerevisiae all have commercially available.
Embodiment 1
Bacillus subtilis, S. cervisiae, Lactobacillus plantarum be the raw material composition of 1:1:1 by weight.Wherein said bacillus subtilis viable count (1.0 × 10
10cfu/g), S. cervisiae viable count is (1.0 × 10
10cfu/g), Lactobacillus plantarum viable count is (1.0 × 10
10cfu/g).
Bacillus subtilis consumption is 0.2% (w/w), S. cervisiae consumption 0.2% (w/w), Lactobacillus plantarum consumption 0.2% (w/w).
Embodiment 2
Bacillus subtilis, S. cervisiae, Lactobacillus plantarum be the raw material composition of 1:1:1 by weight.Wherein said bacillus subtilis viable count (1.0 × 10
10cfu/g), S. cervisiae viable count is (1.0 × 10
10cfu/g), Lactobacillus plantarum viable count is (1.0 × 10
10cfu/g).
Bacillus subtilis consumption is 0.5% (w/w), S. cervisiae consumption 0.5% (w/w), Lactobacillus plantarum consumption 0.5% (w/w).
Embodiment 3
Bacillus subtilis, S. cervisiae, Lactobacillus plantarum be the raw material composition of 1:1:1 by weight.Wherein said bacillus subtilis viable count (1.0 × 10
10cfu/g), bacterium viable count is (1.0 × 10
10cfu/g), Lactobacillus plantarum viable count is (1.0 × 10
10cfu/g).
Bacillus subtilis consumption is 0.9% (w/w), S. cervisiae consumption 0.9% (w/w), Lactobacillus plantarum consumption 0.9% (w/w).
Claims (5)
1. a compound micro-ecological preparation, it is characterized in that it by bacillus subtilis, S. cervisiae, Lactobacillus plantarum by weight the raw material of 1:1:1 form, wherein said bacillus subtilis viable count (1.0 × 10
10cfu/g), S. cervisiae viable count is (1.0 × 10
10cfu/g), Lactobacillus plantarum viable count is (1.0 × 10
10cfu/g).
2. compound micro-ecological preparation claimed in claim 1, wherein bacillus subtilis consumption 0.2-0.9% (w/w), S. cervisiae consumption 0.2-0.9% (w/w), Lactobacillus plantarum consumption 0.2-0.9% (w/w).
3. the application of the compound micro-ecological preparation described in claim 1 or 2 aspect reduction feces of livestock and poultry environmental pollution.
4. the application of the compound micro-ecological preparation described in claim 1 or 2 aspect the dirty the content of nitrogen and phosphorous of reduction child care pig manure.
5. application claimed in claim 4, the dirty the content of nitrogen and phosphorous of wherein said reduction child care pig manure refers to: effectively improve the apparent digestibility of nitrogen, phosphorus, reduce the content of nitrogen and phosphorous in ight soil.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961865A (en) * | 2016-05-11 | 2016-09-28 | 山东华牧天元农牧股份有限公司 | Compound micro-ecological preparation and application thereof |
| CN109679883A (en) * | 2019-01-31 | 2019-04-26 | 天津市畜牧兽医研究所 | It is a kind of improve lactic acid content alfalfa ensilage leavening and alfalfa ensilage preparation method |
| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
| CN112369530A (en) * | 2020-11-17 | 2021-02-19 | 广东恒兴饲料实业股份有限公司 | Green low-emission meat duck feed and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234623A (en) * | 2011-03-13 | 2011-11-09 | 沧州市中信生物科技有限公司 | Microbial preparation and fermentation process thereof |
| CN102488089A (en) * | 2011-11-18 | 2012-06-13 | 吉林省农业科学院 | Preparation method of microecological culture feed additive |
| CN103436473A (en) * | 2013-08-23 | 2013-12-11 | 福州大用生物应用科技有限公司 | Composite micro-ecological preparation for pig breeding healthcare |
| CN103466910A (en) * | 2013-08-23 | 2013-12-25 | 福州大用生物应用科技有限公司 | Compound micro ecological bacteria agent used for live pig excrement treatment |
| CN103478443A (en) * | 2013-08-20 | 2014-01-01 | 沈阳金科丰牧业科技有限公司 | Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler |
| CN103509743A (en) * | 2013-09-09 | 2014-01-15 | 深圳市三盛环保科技有限公司 | Microbial compound preparation and preparation method |
-
2014
- 2014-03-25 CN CN201410112422.8A patent/CN103843974A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234623A (en) * | 2011-03-13 | 2011-11-09 | 沧州市中信生物科技有限公司 | Microbial preparation and fermentation process thereof |
| CN102488089A (en) * | 2011-11-18 | 2012-06-13 | 吉林省农业科学院 | Preparation method of microecological culture feed additive |
| CN103478443A (en) * | 2013-08-20 | 2014-01-01 | 沈阳金科丰牧业科技有限公司 | Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler |
| CN103436473A (en) * | 2013-08-23 | 2013-12-11 | 福州大用生物应用科技有限公司 | Composite micro-ecological preparation for pig breeding healthcare |
| CN103466910A (en) * | 2013-08-23 | 2013-12-25 | 福州大用生物应用科技有限公司 | Compound micro ecological bacteria agent used for live pig excrement treatment |
| CN103509743A (en) * | 2013-09-09 | 2014-01-15 | 深圳市三盛环保科技有限公司 | Microbial compound preparation and preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 晁晓静: "动物饲养过程中造成环境污染的原因", 《河南畜牧兽医》, vol. 29, no. 8, 31 December 2008 (2008-12-31), pages 11 - 13 * |
| 汪嘉燮等: "《生态养猪技术》", 29 February 2012, article "利用益生菌，改善猪体内微生态环境", pages: 49 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961865A (en) * | 2016-05-11 | 2016-09-28 | 山东华牧天元农牧股份有限公司 | Compound micro-ecological preparation and application thereof |
| CN105961865B (en) * | 2016-05-11 | 2020-02-07 | 山东华牧天元农牧股份有限公司 | Composite microecological preparation and application |
| CN109679883A (en) * | 2019-01-31 | 2019-04-26 | 天津市畜牧兽医研究所 | It is a kind of improve lactic acid content alfalfa ensilage leavening and alfalfa ensilage preparation method |
| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
| CN112369530A (en) * | 2020-11-17 | 2021-02-19 | 广东恒兴饲料实业股份有限公司 | Green low-emission meat duck feed and preparation method thereof |
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Application publication date: 20140611 |