CN103830331B - A kind of preparation method pressing down dermatitis preparation - Google Patents
A kind of preparation method pressing down dermatitis preparation Download PDFInfo
- Publication number
- CN103830331B CN103830331B CN201410096829.6A CN201410096829A CN103830331B CN 103830331 B CN103830331 B CN 103830331B CN 201410096829 A CN201410096829 A CN 201410096829A CN 103830331 B CN103830331 B CN 103830331B
- Authority
- CN
- China
- Prior art keywords
- mixture
- preparation
- dermatitis
- gorgon euryale
- revs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses and a kind ofly press down the preparation method of dermatitis preparation, belong to biological technical field, main technique methods is as follows: Gorgon euryale seed to be first immersed in alcoholic solution stir process 20-50 minute after 30-90 minute; Adopt Filtration collect stir after liquid, under 37 DEG C of conditions after oscillation treatment 6-10 hour to get supernatant after at least 8000 revs/min of centrifugation, by centrifugal supernatant ethanol removing, obtain dense thick liquid; By for dense fluids of extraction with 0.9% normal saline fully dissolve, obtain Semen Euryales kind shell extract solution; Fructus Benincasae frost is added in 0.9% normal saline, under 37 DEG C of conditions, centrifugal after vibration, collect centrifugal supernatant and namely obtain Fructus Benincasae frost solution; Obtain one after Semen Euryales kind shell extract solution, Fructus Benincasae frost solution and vitamin C are carried out proportioning and press down dermatitis preparation.The inventive method has green, nuisanceless, safe advantage, can be applicable to suppress dermatitis reaction.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of preparation method pressing down dermatitis preparation.
Technical background
Dermatitis has another name called eczema, is a kind of dermal inflammatory disease.The cause of disease of dermatitis is varied, is broadly divided into: several large class of mechanical friction, fungal infectious, chemical substance contact, parasite infectivity, allergenicity etc.Dermatitis morbidity crowd is extensive, and diseased region is wide, and life different phase (infant, childhood period, juvenile era, the manhood, the geratic period) all can have the probability of morbidity.At the beginning of morbidity, usually can feel that site of pathological change pruritus is hard to bear, sense of discomfort is strong, scratch to grab and occur erythema, pimple and the different diseases such as to ooze out afterwards.The present invention investigated a kind of preparation method pressing down dermatitis preparation, is specifically related to from Gorgon euryale seed hull and prepares the method pressing down dermatitis preparation in Fructus Benincasae frost.
Gorgon euryale is the annual large-scale aquatic herbaceous plant of Nymphaeceae, its stem and leaf spinosity, and the fruit of Gorgon euryale is Semen Euryales, and Gorgon euryale seed is rounded, and after ripe, kind shell is hard.The kernel of Gorgon euryale seed is not only edible but also have tonifying speen and tonifying kidney, convergence calmness, reinforcing the kindey to control nocturnal emission, the multiple medical value of spleen reinforcing stopping leak.
Generally, Gorgon euryale processing enterprise is mainly to obtain the kernel of Gorgon euryale for the purpose of commodity selling, and therefore, the shell of most Gorgon euryale seed is all directly abandoned by as waste material.The present invention's research relates to a kind of active substance that extracts from Gorgon euryale seed hull skin and also helps with material such as Fructus Benincasae frost extract, vitamin C etc., prepares the method that effectively can suppress dermatitis material.The present invention has not only opened up the added value of Gorgon euryale processing, and investigated a kind of infant is especially applicable to dermatitis, the preparation method pressing down dermatitis preparation that child's dermatitis has obvious inhibition.But should the active constituent pressed down in dermatitis preparation mainly come from food source property plant, substantially without toxicity, side effect, be a kind of green, safe dermatitis preparation.
The clinical dermatitis material that presses down related in prior art is mainly based on the oral antihistaminic of topical steroid ointment machin.Life-time service steroid ointment medicine or antihistamine drug may produce many drawbacks such as atrophoderma, Marjoram Extract, adrenal gland's suppression, pathobolism, secondary infection and immunity degradation, larger to the toxicity, side effect of infant and child.
Summary of the invention
The object of the present invention is to provide a kind of preparation method pressing down dermatitis preparation, make the dermatitis preparation that presses down obtained have green, safe beneficial characteristics.
In order to solve above technical problem, the concrete technical scheme that the present invention adopts is as follows:
Press down a preparation method for dermatitis preparation, it is characterized in that comprising the following steps:
Step one, puts into container by Gorgon euryale seed, then adds the alcoholic solution of 80%-95% concentration, makes the alcoholic solution that adds can the Gorgon euryale seed of the just Mei Guo the superiors; Stir 20-50 minute again after soaking 30-90 minute under room temperature, obtain mixture one;
Step 2, filters mixture one, obtains mixture two after removing Gorgon euryale seed;
Step 3, is placed in the oscillation device of 120-160 rev/min by described mixture two, vibrates and obtain mixture three in 6-10 hour under 37 DEG C of conditions;
Step 4, by mixture three with after the centrifugation of at least 8000 revs/min, collects the centrifugal supernatant of gained, then eliminates ethanol in centrifugal supernatant with Rotary Evaporators, obtain dense thick liquid and mixture four;
Step 5, mixture four is added concentration be 0.9% normal saline fully dissolve, mixture four is 1g:10--100ml with the proportionate relationship of normal saline; Namely mixture five is obtained after dissolving completely;
Step 6, the outer field white lead of scraping Exocarpium Benincasae and Fructus Benincasae frost 1-5g, add the normal saline of 0.9% concentration of 10-50ml, place under 37 DEG C of conditions in the oscillation device of 120-160 rev/min, vibrate 2-4 hour with the centrifugation of at least 8000 revs/min, collect centrifugal supernatant and namely obtain mixture six;
Step 7, fully mixes mixture five and mixture six by the mass ratio of 10:1, obtains mixture seven;
Step 8, mixes mixture seven and vitamin C by the mass ratio of 100:1--500:1, must press down dermatitis preparation.
Described oscillation device is agitator or shaking table.
The rotating speed of described oscillation device preferably 120 revs/min.
The present invention has beneficial effect.The present invention presses down the seed that one of dermatitis formulating substances composition mainly comes from water plant Gorgon euryale.Gorgon euryale is the annual plant of growth in Gou Tang, lake, and Gorgon euryale itself has bitter taste, so general little pathogenic infection microorganism, the chance of Gorgon euryale generation disease is also little, so Gorgon euryale is used hardly and uses pesticide in the whole trophophase of a year.In addition, the seed of Gorgon euryale is wrapped in inside Gorgon euryale fruit, only when Gorgon euryale seed maturity the cutting of Gorgon euryale fruit bud, gather after take out the Gorgon euryale seed of the inside, therefore Gorgon euryale seed almost do not reach various microorganism in water and some other water plant leaves withered after corrupt substance.In addition Fructus Benincasae is also the plant of little medication in a kind of whole trophophase, therefore, utilize Gorgon euryale seed hull extract in the present invention and be aided with prepared by Fructus Benincasae frost extract, vitamin C to press down dermatitis material be a kind of green, non-harmful biological preparation.
The dermatitis material that presses down in the present invention mainly comes from a kind of leisure food--Gorgon euryale seed, and the auxiliary substance pressing down dermatitis material comes from the outer field Fructus Benincasae frost of edible Exocarpium Benincasae.Through the social practice of 1,100, up to the present, also do not find that Gorgon euryale seed hull and Fructus Benincasae frost have obvious toxicity, side effect.Therefore, what prepared by the present invention presses down dermatitis preparation is a kind of safe biological preparation.
The present invention is with Gorgon euryale seed hull for main study subject, and therefrom the main constituent pressing down dermatitis preparation is also prepared in extraction further, and this makes Gorgon euryale processing, and refuse--Gorgon euryale kind shell is turned waste into wealth, and considerably increases the added value of Gorgon euryale secondary industry.
Accompanying drawing explanation
Fig. 1 is flow chart of steps of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
Embodiment 1
Step one: install what a electric stirring machine in a bite vat, the center of electric stirring machine has a long quant type stirring rod, each short quant type stirring rod in both sides.The Gorgon euryale seed taking 60 kilograms puts into vat, then adds the alcoholic solution of 80% concentration, makes it the Gorgon euryale seed of the just Mei Guo the superiors.Soak 30 minutes under room temperature, obtain mixture one;
Step 2: opening power, stirs the mixture one 20 minutes.Cross the Gorgon euryale seed in the mixture after filtering stirring one, collect the liquid mixture after filtering, obtain mixture two;
Step 3: in the agitator that mixture two is placed on 120 revs/min or shaking table, carry out oscillation treatment under 37 DEG C of conditions about 6 hours, obtain mixture three;
Step 4: by mixture three with 8000 revs/min of centrifugation, collect centrifugal after supernatant, with Rotary Evaporators removing centrifuged supernatant in ethanol, obtain dense thick liquid and mixture four;
Step 5: add in 1.5g mixture four 15ml concentration be 0.9% normal saline carry out diluting, dissolve after obtain mixture five;
Step 6, the outer field white lead of scraping Exocarpium Benincasae and Fructus Benincasae frost 1g, add the normal saline of 0.9% concentration of 10ml, place in the oscillation device of 120 revs/min under 37 DEG C of conditions, to vibrate after 2 hours with the centrifugation of 8000 revs/min, collect centrifugal supernatant and namely obtain mixture six;
20 grams of mixture five and 2 grams of mixture six are fully mixed, obtain mixture seven by step 7;
15 grams of mixture seven and 150 milligrams of vitamin Cs are mixed, must press down dermatitis preparation by step 8.
Embodiment 2
Step one: install what a electric stirring machine in a bite vat.The Gorgon euryale seed taking 80 kilograms puts into vat, then adds the alcoholic solution of 90% concentration, makes it the Gorgon euryale seed of the just Mei Guo the superiors.Soak 60 minutes under room temperature, obtain mixture one;
Step 2: opening power, stirs the mixture one 35 minutes.Cross the Gorgon euryale seed in the mixture after filtering stirring one, collect the liquid mixture after filtering, obtain mixture two;
Step 3: in the agitator that mixture two is placed on 140 revs/min or shaking table, carry out oscillation treatment under 37 DEG C of conditions about 8 hours, obtain mixture three;
Step 4: by mixture three with 10000 revs/min of centrifugation, collect centrifugal after supernatant, with Rotary Evaporators removing centrifuged supernatant in ethanol, obtain dense thick liquid and mixture four;
Step 5: add in 0.5g mixture four 25ml concentration be 0.9% normal saline fully dissolve after obtain mixture five;
Step 6, the outer field white lead of scraping Exocarpium Benincasae and Fructus Benincasae frost 2.5g, add the normal saline of 0.9% concentration of 50ml, place in the oscillation device of 140 revs/min under 37 DEG C of conditions, to vibrate after 3 hours with the centrifugation of 10000 revs/min, collect centrifugal supernatant and namely obtain mixture six;
50 grams of mixture five and 5 grams of mixture six are fully mixed, obtain mixture seven by step 7;
50 grams of mixture seven and 200 milligrams of vitamin Cs are mixed by step 8, namely obtain one and press down dermatitis preparation.
Embodiment 3
Step one: install what a electric stirring machine in a bite vat.The Gorgon euryale seed taking 90 kilograms puts into vat, then adds the alcoholic solution of 95% concentration, makes it the Gorgon euryale seed of the just Mei Guo the superiors.Soak 90 minutes under room temperature, obtain mixture one;
Step 2: opening power, stirs the mixture one 50 minutes.Cross the Gorgon euryale seed in the mixture after filtering stirring one, collect the liquid mixture after filtering, obtain mixture two;
Step 3: in the agitator that mixture two is placed on 160 revs/min or shaking table, carry out oscillation treatment under 37 DEG C of conditions about 10 hours, obtain mixture three;
Step 4: by mixture three with 12000 revs/min of centrifugation, collect centrifugal after supernatant, with Rotary Evaporators removing centrifuged supernatant in ethanol, obtain dense thick liquid and mixture four;
Step 5: add in 1g mixture four 100ml concentration be 0.9% normal saline carry out diluting, dissolving, obtain mixture five after thing to be mixed fully dissolves;
Step 6, the outer field white lead of scraping Exocarpium Benincasae and Fructus Benincasae frost 1g, add the normal saline of 0.9% concentration of 50ml, place in the oscillation device of 160 revs/min under 37 DEG C of conditions, to vibrate after 4 hours with the centrifugation of 12000 revs/min, collect centrifugal supernatant and namely obtain mixture six;
50 grams of mixture five and 5 grams of mixture six are fully mixed, obtain mixture seven by step 7;
50 grams of mixture seven and 100 milligrams of vitamin Cs are mixed by step 8, namely obtain one and press down dermatitis preparation.
The example pressing down the dermatitis reaction of dermatitis material suppression flu rear nose position of preparation in embodiment 1
(1) suffer from the volunteer of strong cold, within continuous several days, constantly flow clear nasal mucus, cause the dermatitis at position in its nose position and people.Volunteer's dermatitis area skin severe redness, when wiping clear nasal mucus, pain is strong;
(2) aseptic cotton carrier is put into Gorgon euryale seed hull skin ethanol extraction solution and make the abundant draw solution of cotton swab head, then use cotton swab wiping 2-5 time back and forth gently at volunteer's nose and the obvious position of the medium dermatitis symptom of people.Every 4 hours, wiping was once again;
(3) through 3-6 coating, the rubescent situation at volunteer's dermatitis position and pain have to be improved significantly, does not have the senses of discomfort such as obvious burning pain during the clear nasal mucus of wiping.
The example pressing down the reaction of dermatitis material suppression children's perianal dermatitis of preparation in embodiment 3
Suffering from the child of perianal dermatitis about (1) 8 week age, there is obvious redness in its dermatitis position, and has little erythema to occur, simultaneously with burning sensation, pain.Child's readme dermatitis position sense of discomfort is strong, and impact is normally walked;
(2) aseptic cotton carrier is put into the dermatitis material that presses down prepared by embodiment 3 and make the abundant draw solution of cotton swab head, then use cotton swab wiping 2-5 time back and forth gently at dermatitis position.Every 4 hours, wiping was once again;
(3) through 3 coatings, the rubescent situation at the dermatitis position of child and pain, burning sensation have to be improved significantly, and the erythema at the scorching position of raw hide also diminishes.The sense of discomfort caused because of dermatitis disappears substantially, and child can normally walk.
Claims (3)
1. press down a preparation method for dermatitis preparation, it is characterized in that comprising the following steps:
Step one, puts into container by Gorgon euryale seed, then adds the alcoholic solution of 80%-95% concentration, makes the alcoholic solution that adds can the Gorgon euryale seed of the just Mei Guo the superiors; Stir 20-50 minute again after soaking 30-90 minute under room temperature, obtain mixture one;
Step 2, filters mixture one, obtains mixture two after removing Gorgon euryale seed;
Step 3, is placed in the oscillation device of 120-160 rev/min by described mixture two, vibrates and obtain mixture three in 6-10 hour under 37 DEG C of conditions;
Step 4, by mixture three with after the centrifugation of at least 8000 revs/min, collects the centrifugal supernatant of gained,
Eliminate ethanol in centrifugal supernatant with Rotary Evaporators again, obtain dense thick liquid and mixture four;
Step 5, mixture four being added concentration is that the normal saline of 0.9 % fully dissolves, and mixture four is 1 g:10--100ml with the proportionate relationship of normal saline; Namely mixture five is obtained after dissolving completely;
Step 6, the outer field white lead of scraping Exocarpium Benincasae and Fructus Benincasae frost 1-5g, add the normal saline of 0.9% concentration of 10-50 ml, place under 37 DEG C of conditions in the oscillation device of 120-160 rev/min, vibrate 2-4 hour with the centrifugation of at least 8000 revs/min, collect centrifugal supernatant and namely obtain mixture six;
Step 7, fully mixes mixture five and mixture six by the mass ratio of 10:1, obtains mixture seven;
Step 8, mixes mixture seven and vitamin C by the mass ratio of 100:1--500:1, must press down dermatitis preparation.
2. a kind of preparation method pressing down dermatitis preparation according to claim 1, is characterized in that: described oscillation device is agitator or shaking table.
3. a kind of preparation method pressing down dermatitis preparation according to claim 1, is characterized in that: the rotating speed of described oscillation device preferably 120 revs/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410096829.6A CN103830331B (en) | 2014-03-17 | 2014-03-17 | A kind of preparation method pressing down dermatitis preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410096829.6A CN103830331B (en) | 2014-03-17 | 2014-03-17 | A kind of preparation method pressing down dermatitis preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103830331A CN103830331A (en) | 2014-06-04 |
| CN103830331B true CN103830331B (en) | 2015-09-23 |
Family
ID=50794434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410096829.6A Active CN103830331B (en) | 2014-03-17 | 2014-03-17 | A kind of preparation method pressing down dermatitis preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103830331B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105147781B (en) * | 2015-04-03 | 2019-08-27 | 江苏大学 | One kind is with the scorching anti-allergy preparation of Gorgon euryale kind shell extract suppression as main component and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526587A (en) * | 2011-12-18 | 2012-07-04 | 鞠春燕 | Chinese medicinal combination for treating seborrhoeic dermatitis |
| CN103263545A (en) * | 2013-04-22 | 2013-08-28 | 缪友道 | Traditional Chinese medicine for treating seborrheic dermatitis |
-
2014
- 2014-03-17 CN CN201410096829.6A patent/CN103830331B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526587A (en) * | 2011-12-18 | 2012-07-04 | 鞠春燕 | Chinese medicinal combination for treating seborrhoeic dermatitis |
| CN103263545A (en) * | 2013-04-22 | 2013-08-28 | 缪友道 | Traditional Chinese medicine for treating seborrheic dermatitis |
Non-Patent Citations (1)
| Title |
|---|
| 中药内服外治治疗脂溢性皮炎80例临床观察;魏静;《新疆中医药》;20081231;第26卷(第04期);33－34 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103830331A (en) | 2014-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104522167A (en) | Passion fruit milk beverage and preparation method thereof | |
| CN104560931B (en) | A kind of extracting method of glusulase | |
| CN104686725A (en) | Cyclocarya paliurus health tea and making method thereof | |
| CN103913564A (en) | Method for testing bacteriostatic activity of spire wampee extractive | |
| CN105326706A (en) | Hand sanitizer containing tea seed cake extract and preparation method of hand sanitizer | |
| CN103830331B (en) | A kind of preparation method pressing down dermatitis preparation | |
| CN103385835A (en) | Preparation method of dittany bark hand sanitizer | |
| CN105055243A (en) | Hypericum japonicum shower gel capable of clearing heat and promoting dieresis and preparation method thereof | |
| CN105748358B (en) | A kind of agalloch eaglewood essence assigns running water and preparation method thereof | |
| CN104642715A (en) | Method for extracting rice germ protein | |
| CN106854516A (en) | It is a kind of except formaldehyde infantile clothes machine-wash liquid compound method | |
| CN103160392A (en) | Method for preparing non-toxic fruit and vegetable detergent | |
| CN103773596B (en) | The preparation method of krill oil | |
| CN105815440A (en) | Common tulip flower extract-containing fruit and vegetable fresh-keeping agent and preparation method thereof | |
| JP3535151B1 (en) | Method for producing liquid preparation containing live yeast component and liquid preparation | |
| CN104027397B (en) | A kind of preparation method of integration of edible and medicinal herbs plant extract anti-sensitizer and application | |
| CN102228248A (en) | Energy-saving and environmentally-protected production process of Songzhen capsules | |
| KR101621164B1 (en) | Soap and a soap manufacturing method containing cheonmundong | |
| CN108042457A (en) | A kind of anti-acne facial cleanser and preparation method thereof | |
| CN113881607A (en) | Research and development method for preventing and treating avian clostridium perfringens | |
| CN103005426A (en) | Preparation method for gingko biloba sarcotesta extracting solution | |
| CN106720244A (en) | A kind of antistaling agent and preparation method thereof | |
| CN110679946A (en) | Preparation method of radix puerariae essence electuary | |
| CN106667885A (en) | Tear-free pet dot bath foam | |
| CN107582443A (en) | The production method of Closterium mildy wash |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |