CN103815134A - Feed and traditional Chinese medicine composition for treating porcine pathogenic Escherichia coli diseases - Google Patents
Feed and traditional Chinese medicine composition for treating porcine pathogenic Escherichia coli diseases Download PDFInfo
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- CN103815134A CN103815134A CN201410078818.5A CN201410078818A CN103815134A CN 103815134 A CN103815134 A CN 103815134A CN 201410078818 A CN201410078818 A CN 201410078818A CN 103815134 A CN103815134 A CN 103815134A
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a traditional Chinese medicine composition for treating porcine pathogenic Escherichia coli diseases. The raw material medicines comprise common andrographis herb, scutellaria baicalensis, white paeony root, cacumen biotae, hempleaf negundo chastetree leaf, the root of Chinese pulsatilla, codonopsis pilosula, radix bupleuri, madder, smoked plum, centipeda minima, hawthorn, pericarpium citri reticulatae, rhizoma polygonati, rhizoma kaempferiae, honeysuckle stem, rhizoma galangae, adenophora tetraphylla, gorgon fruit, corydalis tuber, folium ginkgo, fringed pink and semen raphani. The traditional Chinese medicine composition provided in the invention has the advantages of bacteria resistance, virus resistance, small side effects, zero residue and no drug resistance, is a good substitute of antibiotics and has a wide development prospect in the aspect of treating the porcine pathogenic Escherichia coli diseases.
Description
Technical field
The present invention relates to traditional Chinese medicine technical field, relate in particular to a kind of feed and Chinese medicine composition that is used for the treatment of pig enteropathogenic E．Coli disease.
Background technology
Pig enteropathogenic E．Coli disease is a kind of acute infectious disease being caused by pig enteropathogenic E．Coli disease, the clinical diarrhea of pigs that mainly causes, main manifestations is the various clinical symptoms such as enteritis, enterotoxemia, this disease all has generation all over the world, particularly large quantities of death or the retarded growth of newborn piglet are caused in the In Guangdong Province of pig industry prosperity in China, having become one of serious hindrance of development pig industry, is also to cause one of the disease of dealer's economic loss maximum of raising pigs.It all can occur throughout the year, and without seasonal, severe winter and hot summer, moist rainy morbidity in season was many.The morbidity of pig intensive farm is serious, disperses the pig of raising to fall ill few.Pig enteropathogenic E．Coli disease is distributes or endemicity, and the incidence of disease, fatal rate are all very high.
The control of this disease mainly relies on the chemicalses such as antibiotic.But a large amount of uses of antibiotic etc cause that medicament residue and bacterium, virus produce drug resistance, further strengthen the difficulty of disease control.Due to the long-term use of antimicrobial, also can make pathogen produce drug resistance.In addition the medicament residue that, long-term medication causes has also brought harm to human health.
Traditional Chinese veterinary medicine has long history and abundant application experience, before antibiotic uses, to ensureing that animal health plays an important role.Along with animal husbandry scale, intensive development; and the long-term abuse of antibiotic and the pathogen resistance to the action of a drug that causes strengthens while manifesting with medicament residue problem increasingly; Chinese medicine is little with antibacterial, disease-resistant toxicity, side effect, noresidue, the feature such as have no drug resistance, and has become substitute antibiotics and has solved drug resistance and the residual important aspect of medicine.
The invention provides a kind of Chinese medicine composition that is used for the treatment of pig enteropathogenic E．Coli disease, its antibacterial, disease-resistant toxicity, side effect is little, noresidue, have no drug resistance, and is antibiotic favorable substitutes, has vast potential for future development.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine composition that is used for the treatment of pig enteropathogenic E．Coli disease, and its antibacterial, disease-resistant toxicity, side effect is little, noresidue, have no drug resistance, and is antibiotic favorable substitutes, has vast potential for future development.
For solving the problems of the technologies described above, the invention provides a kind of feed that is used for the treatment of pig enteropathogenic E．Coli disease, raw material comprises corn, wheat bran, peanut meal, the dish dregs of rice, fish meal, calcium monohydrogen phosphate, salt, methionine, threonine, vitamin and is used for the treatment of the sick Chinese medicine composition additive of pig enteropathogenic E．Coli.
Wherein, in described feed, the weight of each raw material is respectively corn 7kg～9kg, wheat bran 2kg～3kg, peanut meal 0.5kg～0.7kg, dish dregs of rice 0.8kg～1.2kg, fish meal 0.5kg～0.7kg, calcium monohydrogen phosphate 0.05kg～0.15kg, salt 0.05kg～0.15kg, methionine 0.01kg～0.03kg, threonine 0.01kg～0.03kg, vitamin 0.1kg～0.2kg, Chinese medicine composition additive 1kg～1.5kg.
Wherein, in described feed, the weight of each raw material is respectively corn 8kg, wheat bran 2.5kg, peanut meal 0.6kg, dish dregs of rice 1kg, fish meal 0.6kg, calcium monohydrogen phosphate 0.1kg, salt 0.1kg, methionine 0.02kg, threonine 0.02kg, vitamin 0.15kg, Chinese medicine composition additive 1.2kg.
Wherein, the bulk drug in described Chinese medicine composition additive comprises Herba Andrographitis, the root of large-flowered skullcap, the root of herbaceous peony, cacumen biotae, leaf of negundo chastetree, the Chinese bulbul, Radix Codonopsis, radix bupleuri, madder, dark plum, Centipeda minima, hawthorn, dried orange peel, sealwort, Kaempferia galanga, caulis lonicerae, galangal, adenophora tetraphylla, Gorgon fruit, corydalis tuber, ginkgo leaf, fringed pink and radish seed.
Wherein, in described Chinese medicine composition additive, the weight of each bulk drug is respectively Herba Andrographitis 20g～30g, root of large-flowered skullcap 55g～65g, root of herbaceous peony 25g～35g, cacumen biotae 25g～35g, leaf of negundo chastetree 20g～30g, Chinese bulbul 35g～45g, Radix Codonopsis 30g～40g, radix bupleuri 20g～30g, madder 30g～40g, dark plum 25g～35g, Centipeda minima 20g～30g, hawthorn 25g～35g, dried orange peel 15g～25g, sealwort 35g～45g, Kaempferia galanga 20g～30g, caulis lonicerae 30g～40g, galangal 20g～30g, adenophora tetraphylla 25g～35g, Gorgon fruit 20g～30g, corydalis tuber 15g～25g, ginkgo leaf 30g～40g, fringed pink 25g～35g and radish seed 25g～35g.
The present invention also provides a kind of Chinese medicine composition that is used for the treatment of pig enteropathogenic E．Coli disease, and its bulk drug comprises Herba Andrographitis, the root of large-flowered skullcap, the root of herbaceous peony, cacumen biotae, leaf of negundo chastetree, the Chinese bulbul, Radix Codonopsis, radix bupleuri, madder, dark plum, Centipeda minima, hawthorn, dried orange peel, sealwort, Kaempferia galanga, caulis lonicerae, galangal, adenophora tetraphylla, Gorgon fruit, corydalis tuber, ginkgo leaf, fringed pink and radish seed.
Wherein, in described Chinese medicine composition, the weight of each bulk drug is respectively Herba Andrographitis 20g～30g, root of large-flowered skullcap 55g～65g, root of herbaceous peony 25g～35g, cacumen biotae 25g～35g, leaf of negundo chastetree 20g～30g, Chinese bulbul 35g～45g, Radix Codonopsis 30g～40g, radix bupleuri 20g～30g, madder 30g～40g, dark plum 25g～35g, Centipeda minima 20g～30g, hawthorn 25g～35g, dried orange peel 15g～25g, sealwort 35g～45g, Kaempferia galanga 20g～30g, caulis lonicerae 30g～40g, galangal 20g～30g, adenophora tetraphylla 25g～35g, Gorgon fruit 20g～30g, corydalis tuber 15g～25g, ginkgo leaf 30g～40g, fringed pink 25g～35g and radish seed 25g～35g.
More preferably Herba Andrographitis 25g, root of large-flowered skullcap 60g, root of herbaceous peony 30g, cacumen biotae 30g, leaf of negundo chastetree 25g, Chinese bulbul 40g, Radix Codonopsis 35g, radix bupleuri 25g, madder 35g, dark plum 30g, Centipeda minima 25g, hawthorn 30g, dried orange peel 20g, sealwort 40g, Kaempferia galanga 25g, caulis lonicerae 35g, galangal 25g, adenophora tetraphylla 30g, Gorgon fruit 25g, corydalis tuber 20g, ginkgo leaf 35g, fringed pink 30g and radish seed 30g of each raw material in described Chinese medicine composition.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, is specially:
Each bulk drug component is mixed by weight, obtain mixture, add determining alcohol and be 50%～70% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 2h～3h, the amount that the mixture of every 1kg adds ethanolic solution is 3L～5L, the extract obtaining merges and filters, and leaves standstill 24h, reclaims ethanol concentrated, be condensed into thick paste, dry, pulverize into fine powder, obtain Chinese medicine composition.
The present invention also provides the preparation method of above-mentioned feed, and it comprises:
The first step, mixes the each bulk drug component in described Chinese medicine composition additive by weight, obtains mixture, add determining alcohol and be 50%～70% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 2h～3h, the amount that the mixture of every 1kg adds ethanolic solution is 3L～5L, the extract of acquisition merges and filters, leave standstill 24h, reclaim ethanol concentrated, be condensed into thick paste, dry, be ground into fine powder, obtain described Chinese medicine composition additive;
Second step, gets the each raw material except described Chinese medicine composition additive in described feed and first pulverizes, and then the raw material after pulverizing is put into mixer and mixes, add again Chinese medicine composition additive prepared by the first step further to mix, mixed material enters modulator, and refining temperature is 70 ℃, modulating time 10 seconds, after modulation, send into slaking tank and carry out slaking, the curing time is 5 minutes, 70 ℃ of curing temperatures, finally enter pellet fabrication device and granulate, obtain finished product feedstuff.
Beneficial effect of the present invention:
Its antibacterial, disease-resistant toxicity, side effect of Chinese medicine composition provided by the invention is little, noresidue, have no drug resistance, and is antibiotic favorable substitutes, has vast potential for future development at treatment pig enteropathogenic E．Coli aspect sick.
The specific embodiment
The present invention is take clearing heat and detoxicating, inducing diuresis for removing edema, relieving diarrhea with astringents as principle of reatment, a kind of Chinese medicine composition that is used for the treatment of pig enteropathogenic E．Coli disease is provided, and its bulk drug comprises Herba Andrographitis, the root of large-flowered skullcap, the root of herbaceous peony, cacumen biotae, leaf of negundo chastetree, the Chinese bulbul, Radix Codonopsis, radix bupleuri, madder, dark plum, Centipeda minima, hawthorn, dried orange peel, sealwort, Kaempferia galanga, caulis lonicerae, galangal, adenophora tetraphylla, Gorgon fruit, corydalis tuber, ginkgo leaf, fringed pink and radish seed.
Further, described Chinese medicine composition is only made up of above-mentioned raw materials medicine.
In described Chinese medicine composition, the weight of each bulk drug is respectively Herba Andrographitis 20g～30g, root of large-flowered skullcap 55g～65g, root of herbaceous peony 25g～35g, cacumen biotae 25g～35g, leaf of negundo chastetree 20g～30g, Chinese bulbul 35g～45g, Radix Codonopsis 30g～40g, radix bupleuri 20g～30g, madder 30g～40g, dark plum 25g～35g, Centipeda minima 20g～30g, hawthorn 25g～35g, dried orange peel 15g～25g, sealwort 35g～45g, Kaempferia galanga 20g～30g, caulis lonicerae 30g～40g, galangal 20g～30g, adenophora tetraphylla 25g～35g, Gorgon fruit 20g～30g, corydalis tuber 15g～25g, ginkgo leaf 30g～40g, fringed pink 25g～35g and radish seed 25g～35g.
More preferably Herba Andrographitis 25g, root of large-flowered skullcap 60g, root of herbaceous peony 30g, cacumen biotae 30g, leaf of negundo chastetree 25g, Chinese bulbul 40g, Radix Codonopsis 35g, radix bupleuri 25g, madder 35g, dark plum 30g, Centipeda minima 25g, hawthorn 30g, dried orange peel 20g, sealwort 40g, Kaempferia galanga 25g, caulis lonicerae 35g, galangal 25g, adenophora tetraphylla 30g, Gorgon fruit 25g, corydalis tuber 20g, ginkgo leaf 35g, fringed pink 30g and radish seed 30g of each raw material in described Chinese medicine composition.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, is specially:
Each bulk drug component is mixed by weight, obtain mixture, add determining alcohol and be 50%～70% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 2h～3h, the amount that the mixture of every 1kg adds ethanolic solution is 3L～5L, the extract obtaining merges and filters, and leaves standstill 24h, reclaims ethanol concentrated, be condensed into thick paste, dry, pulverize into fine powder, obtain Chinese medicine composition.
In described Chinese medicine composition, the pharmacology of each bulk drug is as follows:
Herba Andrographitis: bitter, cold in nature, the thoughts of returning home, lung, large intestine, urinary bladder channel, have clearing heat and detoxicating, cool blood, detumescence, effect of eliminating dampness, cures mainly cold, fever, abscess of throat, aphthae, pertussis labor is coughed, dysentery, heat is drenched puckery pain, the carbuncle sore that swells, the cards such as venomous snake bite.
The root of large-flowered skullcap: bitter, cold in nature, enter the heart, lung, stomach, courage, large intestine channel, there is heat-clearing and damp-drying drug, purging intense heat and detonicating, antiabortive, the effect of hemostasis, the diarrhoea, dysentery, jaundice, the heat that are mainly used in due to damp and hot are drenched; Cough with lung heat, thirsty pharyngalgia, also can be used for fetal movement with fever, and hot malicious fire-evil is invaded the swollen card of sore carbuncle poison of excessive skin.
The root of herbaceous peony: bitter, acid, cool in nature, enter liver, the spleen channel, there is nourishing blood and liver, slow middle pain relieving, astringe yin is received effect of sweat, cures mainly chest abdomen pain over the hypochondriac region, rushes down dysentery stomachache, spontaneous sweating, fever due to yin deficiency, irregular menstruation, uterine bleeding, is with inferior card.
Cacumen biotae: bitter, puckery, cold in nature, enter the heart, liver, large intestine channel, there is cooling blood and hemostasis, wines used as antirheumatic, effect of loose pyogenic infections, cures mainly haematemesis, bleeding from five sense organs or subcutaneous tissue, hematuria, bloody flux, intestines wind, uterine bleeding, arthralgia due to wind-dampness, bacillary dysentery, hypertension, cough, erysipelas, mumps, the cards such as scald.
Leaf of negundo chastetree: taste is pungent; Bitter; Property is put down, and returns lung channel, has the dampness elimination of inducing sweat, eliminating phlegm and relieving asthma; Removing toxic substances, expels pathogenic wind from the body surface, dehumidifying, and desinsection, effect of pain relieving, cures mainly cold, cough, asthma; Stomachache; Stomachache; Summer-heat and damp is rushed down dysentery; Beriberi swelling; Rubella itch; The ringworm of the foot; Acute mastitis swells and ache; The cards such as snake bite and insect sting.
The Chinese bulbul: bitter, cold in nature, enter stomach, large intestine channel, there is effect clearing heat and detoxicating, that remove heat from the blood and relieve diarrhea, being mainly used in stomach has damp and hot stagnant heating stomachache, diarrhea purulence blood, the tenesmus card that waits.
Radix Codonopsis: taste is sweet, property is flat, enters lung, the spleen channel, has tonifying Qi benefit spleen, and the effect of nourishing blood to promote the production of body fluid, is mainly used in spleen-lung Qi deficiency or qi-blood deficiency lassitude hypodynamia, breathes hard, the cards such as spontaneous perspiration of coughing.
Radix bupleuri: bitter, cold nature, returns liver, gallbladder channel, has to evacuate to bring down a fever, and rises relax effect of liver of sun, cures mainly cold, fever, fevers and chills alternate, malaria, stagnation of QI due to depression of the liver, sternal rib pain, prolapse of the anus, the card such as uterus comes off, irregular menstruation.
Madder: cold in nature, bitter, returns Liver Channel, effect: cooling blood and hemostasis; Promoting blood circulation and removing blood stasis, cure mainly blood-head spitting of blood, haematemesis, bleeding from five sense organs or subcutaneous tissue, hematuria, have blood in stool, uterine bleeding, through closing, postpartum stasis blocking stomachache, traumatic injury, arthralgia due to wind-dampness, jaundice, sore carbuncle, the hemorrhoid card such as swollen.
Dark plum: bitter, acid, puckery, warm in nature, enter liver, lung, spleen, large intestine channel, have and astringe the lung, puckery intestines, hemostasis, the effect of promoting the production of body fluid, is used for the treatment of protracted dysentery more than, and damp-heat dysentery is had blood in stool, the card such as hematuria, snowslide.
Centipeda minima: taste is pungent, warm in nature, enter lung, Liver Channel, there is cold-dispelling, clearing the nasal passage, cough-relieving apophlegmatic, the effect of eliminating stasis to subdue swelling, for exterior syndrome due to wind-cold, nasal obstruction runny nose and nasosinusitis, coughing with a lot of sputum, pertussis, the cards such as sore, carbuncle and painful swelling.
Hawthorn: taste acid, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, and for food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of faling apart, is mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, have loose bowels, dysentery, hawthorn is the staple for the treatment of indigestion, hawthorn can increase the secretion of peptic digest enzyme, promotes digestion; Shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus etc. are had to obvious inhibitory action.
Dried orange peel: warm in nature, taste is pungent, bitter; Return spleen, lung channel, pungent loose logical temperature, fragrant odour, is longer than and regulates the flow of vital energy, and can enter spleen lung, thus can go loose sluggishness of lung-QI, again can promoting the circulation of qi wide in, for lung qi gather around stagnant, chest diaphragm ruffian full and the card such as the taste stagnation of the circulation of vital energy, abdominal fullness and distention.
Sealwort: taste is sweet, property is flat, enters lung, spleen, kidney channel, has the yin-nourishing of fostering the spirit of nobility, invigorating the spleen moistening lung, effect of enriching kidney essence, for taste deficiency of qi and yin, food is few unable, the lung-dryness syndrome due to yin deficiency, the few phlegm of dry cough, the quenching one's thirst of yin asthenia generating intrinsic heat, the cards such as deficiency of kidney essence, impotence and seminal emission, dizziness and tinnitus, blurred vision dim eyesight.
Kaempferia galanga: taste is pungent, warm in nature, returns stomach warp, has in promoting the circulation of qi temperature, and effect of the pain relieving that helps digestion, cures mainly chest diaphragm turgor, coldness and pain in the epigastrium, the diet card such as do not disappear.
Caulis lonicerae: taste is sweet, cold in nature, attach to the lung and stomach meridians, have clearing heat and detoxicatingly, effect of dispelling wind dredging collateral, cures mainly warm disease heating, toxic-heat and blood stasis, the carbuncle sore that swell, rheumatic fever numbness, card such as redness and swelling of joints heat pain grade.
Galangal: taste is pungent, hot in nature, return spleen, stomach warp, there is warming stomach for dispelling cold, effect of the pain relieving that helps digestion.For coldness and pain in the epigastrium, gastrofrigid vomiting, the cards such as belch acid regurgitation.
Adenophora tetraphylla: sweet, the micro-hardship of taste, cold nature, enters lung, stomach warp, moistens the lung and relieve the cough, and the effect of nourishing the stomach to improve the production of body fluid, is mainly used in the few phlegm of the lung-dryness syndrome due to yin deficiency, dry cough, the labor phlegm blood of coughing, the cards such as deficiency of stomach-Yin, mouthfeel pharynx are dry, constipation, the red few tongue of tongue.
Gorgon fruit: taste is sweet, puckery, property is flat.Return spleen, kidney channel.There is supplementing the kidney to control the nocturnal, tonifying spleen antidiarrheal, clearing damp is the function of band only, cures mainly emission, splenasthenic diarrhea, insufficiency of the spleen gonorrhoea, is with inferior card.
Corydalis tuber: bitter, pungent, warm in nature, return liver, the heart, stomach warp, there is benefaction and wander away, can rise and can fall, have promoting blood circulation to remove blood stasis, effect of promoting qi circulation and relieving pain, cures mainly chest impediment and cardialgia, the side of body rib, all pains of gastral cavity abdomen, headache, pain in the back, hernia pain, muscle and bone ache, dysmenorrhoea, through closing, postpartum, the stasis of blood was suffered from abdominal pain, the cards such as traumatic injury.
Ginkgo leaf: taste is sweet, bitter, puckery, property is flat, enters the heart, lung channel, having astringes the lung relievings asthma, invigorate blood circulation nourish heart, effect of relieving diarrhea with astringents, cough and breathe heavily for the deficiency syndrome of the lung, uncomfortable in chest pained, dizziness headache, has loose bowels, the cards such as dysentery.
Fringed pink: bitter, cold in nature, the thoughts of returning home, small intestinl channel, have inducing diuresis for treating strangurtia, and effect that broken blood stimulates the menstrual flow is drenched for heat, and blood drenches, urolithiasis, urinary obstruction, drenches the puckery pain of drop, the cards such as menstruation apolipsis.
Radish seed: property is flat, and taste is pungent, sweet, returns lung, spleen, stomach warp, disappears and has food de-swelling, and effect of lowering the adverse-rising QI to resolve phlegm cures mainly retention of food and drink, abdominal distention, constipation, the stagnant dysentery, phlegm of rushing down is stopped up the card such as breath with cough.
The present invention also provides a kind of feed for the treatment of pig enteropathogenic E．Coli disease, and raw material comprises corn, wheat bran, peanut meal, the dish dregs of rice, fish meal, calcium monohydrogen phosphate, salt, methionine, threonine, vitamin and above-mentioned Chinese medicine composition additive.
In described feed, the weight of each raw material is respectively corn 7kg～9kg, wheat bran 2kg～3kg, peanut meal 0.5kg～0.7kg, dish dregs of rice 0.8kg～1.2kg, fish meal 0.5kg～0.7kg, calcium monohydrogen phosphate 0.05kg～0.15kg, salt 0.05kg～0.15kg, methionine 0.01kg～0.03kg, threonine 0.01kg～0.03kg, vitamin 0.1kg～0.2kg, Chinese medicine composition additive 1kg～1.5kg.
Further, in described feed, the weight of each raw material is respectively corn 8kg, wheat bran 2.5kg, peanut meal 0.6kg, dish dregs of rice 1kg, fish meal 0.6kg, calcium monohydrogen phosphate 0.1kg, salt 0.1kg, methionine 0.02kg, threonine 0.02kg, vitamin 0.15kg, Chinese medicine composition additive 1.2kg.
Described vitamin can be the multivitamin mixing such as vitamin A, neo dohyfral D3, vitamin E, prokeyvit, vitamin B1, vitamin B2, vitamin B6, cobalamin, nicotinic acid, pantothenic acid, folic acid, also can be vitamin premix, as excellent dimension element (Beijing You Libao biotechnology Co., Ltd provides).
The present invention also provides the preparation method of above-mentioned feed, is specially above-mentioned each raw material of getting except described Chinese medicine composition additive and first pulverizes, and then the raw material after pulverizing is put into mixer and mixes, add described Chinese medicine composition additive, mixed material enters modulator, and refining temperature is 70 ℃, modulating time 10 seconds, after modulation, send into slaking tank and carry out slaking, the curing time is 5 minutes, 70 ℃ of curing temperatures, finally enter pellet fabrication device and granulate, obtain finished product feedstuff.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese medicine composition 1
By Herba Andrographitis 25g, root of large-flowered skullcap 60g, root of herbaceous peony 30g, cacumen biotae 30g, leaf of negundo chastetree 25g, Chinese bulbul 40g, Radix Codonopsis 35g, radix bupleuri 25g, madder 35g, dark plum 30g, Centipeda minima 25g, hawthorn 30g, dried orange peel 20g, sealwort 40g, Kaempferia galanga 25g, caulis lonicerae 35g, galangal 25g, adenophora tetraphylla 30g, Gorgon fruit 25g, corydalis tuber 20g, ginkgo leaf 35g, fringed pink 30g and radish seed 30g mix, obtain mixture, add determining alcohol and be 60% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 3h, the amount that the mixture of every 1kg adds ethanolic solution is 4L, the extract obtaining merges and filters, leave standstill 24h, reclaim ethanol concentrated, be condensed into thick paste, dry, be ground into fine powder, obtain Chinese medicine composition 1.
Embodiment 2 Chinese medicine compositions 2
By Herba Andrographitis 20g, root of large-flowered skullcap 55g, root of herbaceous peony 25g, cacumen biotae 25g, leaf of negundo chastetree 20g, Chinese bulbul 35g, Radix Codonopsis 30g, radix bupleuri 20g, madder 30g, dark plum 25g, Centipeda minima 20g, hawthorn 25g, dried orange peel 15g, sealwort 35g, Kaempferia galanga 20g, caulis lonicerae 30g, galangal 20g, adenophora tetraphylla 25g, Gorgon fruit 20g, corydalis tuber 15g, ginkgo leaf 30g, fringed pink 25g and radish seed 25g mix, obtain mixture, add determining alcohol and be 60% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 3h, the amount that the mixture of every 1kg adds ethanolic solution is 4L, the extract obtaining merges and filters, leave standstill 24h, reclaim ethanol concentrated, be condensed into thick paste, dry, be ground into fine powder, obtain Chinese medicine composition 2.
Embodiment 3 Chinese medicine compositions 3
By Herba Andrographitis 30g, root of large-flowered skullcap 65g, root of herbaceous peony 35g, cacumen biotae 35g, leaf of negundo chastetree 30g, Chinese bulbul 45g, Radix Codonopsis 40g, radix bupleuri 30g, madder 40g, dark plum 35g, Centipeda minima 30g, hawthorn 35g, dried orange peel 25g, sealwort 45g, Kaempferia galanga 30g, caulis lonicerae 40g, galangal 30g, adenophora tetraphylla 35g, Gorgon fruit 30g, corydalis tuber 25g, ginkgo leaf 40g, fringed pink 35g and radish seed 35g mix, obtain mixture, add determining alcohol and be 60% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 3h, the amount that the mixture of every 1kg adds ethanolic solution is 4L, the extract obtaining merges and filters, leave standstill 24h, reclaim ethanol concentrated, be condensed into thick paste, dry, be ground into fine powder, obtain Chinese medicine composition 3.
Embodiment 4 toxicology tests
Acute toxicity test
Property experimental observation, because measuring LD
50therefore, carried out the mensuration of maximum dosage-feeding.Chinese medicine composition prepared by embodiment 1 is dissolved in distilled water, be configured to solution gavage mouse, result shows, with the maximum administration concentration 11.11g of medicine crude drug/ml, maximum administration volume 40ml/kg was administered twice to mouse stomach in 24 hours, mouse maximum dosage-feeding is 888.8g crude drug/kg, and bad reaction and death do not appear in animal.The each internal organs no abnormality seen of anatomic observation.
Long term toxicity test
80 of this test application SD rats, Chinese medicine composition prepared by embodiment 1 is dissolved in distilled water, is configured to solution gavage, establishes control group and 14.5g, 29g, 58g crude drug/kg dosage group, gastric infusion 4 weeks, observes the impact of Chinese medicine composition of the present invention on animal indices continuously.Result of the test shows, successive administration 4 weeks, and the body weight of Chinese medicine composition of the present invention to rat, food ration, peripheral hemogram, clotting time, electrocardiogram, organ index etc. all have no significant effect; Blood biochemical ten binomial index determining results, 4 weeks low dose group BiLi values of administration, may be with wherein three serum sample haemolysis be relevant higher than control group (P < 0.05); Low dose group, middle dosage group TP value be higher than control group (P < 0.05), but all within normal physiological scope, other index and control group be no significant difference relatively.Convalescence each administration group indices measured value and the more equal no significant difference of control group.The each internal organs gross examination of skeletal muscle no abnormality seen of each group.
In sum, the acute toxicity test in mice of this medicine has no toxic reaction, and rat chronic toxicity test also has no overt toxicity reaction.
Embodiment 5 feeds
Get corn 8kg, wheat bran 2.5kg, peanut meal 0.6kg, dish dregs of rice 1kg, fish meal 0.6kg, calcium monohydrogen phosphate 0.1kg, salt 0.1kg, methionine 0.02kg, threonine 0.02kg, vitamin 0.15kg first pulverizes, then the raw material after pulverizing being put into mixer mixes, add Chinese medicine composition additive 1.2kg prepared by embodiment 1 further to mix, mixed material enters modulator, refining temperature is 70 ℃, modulating time 10 seconds, after modulation, send into slaking tank and carry out slaking, curing time is 5 minutes, 70 ℃ of curing temperatures, finally entering pellet fabrication device granulates, obtain finished product feedstuff.
Embodiment 6 extracorporeal bacteria inhibitor tests
Culture medium
Common nutrient broth, ordinary nutrient agar, Mai Kangkai nutrient agar (extensive and profound in meaning star biotechnology responsibility Co., Ltd produces by Beijing).
Strain subject
Escherichia coli for pigs O
20, O
48and O
161from Shandong Agricultural University laboratory, from clinical ill piglet corpse, separate each 1 strain of dominant strain that in warp, prison institute identifies and preserves.
Key instrument equipment
Water isolation type constant incubator, light microscope, ultra low temperature freezer, digital display thermostat water bath, electronic balance.
Culture medium preparation
Common nutrient broth, ordinary nutrient agar culture medium, maconkey agar culture medium are all by shop instruction preparation, and 4 ℃ of Refrigerator stores are stand-by.
The cultivation of enteropathogenic E．Coli
Bacterial classification is inoculated on maconkey agar culture medium, cultivate 24h for 37 ℃, picking colonies typical is inoculated in broth medium, putting 37 ℃ cultivates after 24h, getting each bacterium liquid 0.5 mL is inoculated on General nutrition agar plate and cultivates after 8～12h, with L rod scraping lawn, be diluted to bacteria containing amount 5 × 10 with sterile saline
4～5 × 10
5cFU/mL is for subsequent use.
Bacteriostatic test
Liquid
Chinese medicine composition prepared by embodiment 1 is dissolved in distilled water, obtains the liquid that crude drug concentration is 1g/ml.
Bacteriostatic test
Minimal inhibitory concentration (MIC) is measured
Get 10 of sterilizing test tubes, number consecutively, every pipe first adds sterilizing broth bouillon 1.0 mL, then in the first pipe, adds Chinese traditional medicine water extract 1.0 mL that prepare, mix rear taking-up 1.0 mL and put into the second pipe, the rest may be inferred to the 9th pipe, discards 1mL, and the 10th pipe does not add medicine, as positive control, repeat to prepare three sleeve pipes, get a set of pipe that repeats and do not add dilution of bacteria for negative control pipe, a separate set of repeats to manage a set of a set of experiment use that repeats for subsequent use.Get dilution of bacteria 0.1 mL preparing and add respectively in above-mentioned 1 to 10 pipe and mix, make the 1st pipe to the 9 pipe drug concentrations (mg/mL) be respectively 500,250,125,62.50,31.25,15.60,7.80,3.90,1.95.Put each pipe 37 ℃ of cultivation 24h in constant incubator, observed result.Positive control bacterial growth, is even muddy shape, and when negative control pipe is clarification, transparence without bacterial growth, test is set up, and observes other each pipe situation.
The judgement of bacteriostatic test result
Compare with the corresponding each pipe that repeats control tube and developmental tube and repetition experiment tube, as 2 groups in vitro liquid all clarify, transparent, judge asepsis growth.Clarify another 1 group of muddiness as 1 group, pipette 0.1 mL with the liquid in muddy pipe and go down to posterity and be inoculated in the repetition standby pipes of respective tube, as in second generation liquid in pipe clarification, transparent, judge asepsis growth, as muddiness is determined with bacteria growing.Minimum dilution factor with asepsis growth is minimal inhibitory concentration.MIC<7.82 mg/mL, for extremely sensitive, between 7.82mg/mL～250 mg/mL, is medium sensitivity, and >250 mg/mL, for insensitive.
MBC (MBC) is measured
During above-mentioned MIC is measured each pipe culture respectively setting-out be inoculated in General nutrition agar plate, 37 ℃ of constant temperature culture 24～48h observed results.Clump count is less than 3 and is considered as asepsis growth, with the medicine least concentration without bacterial growth, and the MBC (MBC) as this kind of medicine to this bacterium.
Antibacterial circle diameter is measured
Dip bacterium liquid with sterilized cotton swabs swab, in the even smear inoculation of plain agar planar surface.With aperture 6 mm sterilizing metal card punch punchings, every hole add liquid 0.05 mL left and right to fill it up with not overfolw hole be advisable outward.Do 5 repeat plate, result is averaged.Be placed in 37 ℃ and cultivate 24h, then use vernier caliper measurement antibacterial circle diameter, judge that liquid is to colibacillary fungistatic effect, antibacterial circle diameter <8 mm, for insensitive, between antibacterial circle diameter 8～13 mm, for low responsive, antibacterial circle diameter, between 13～19 mm, is medium sensitivity, antibacterial circle diameter is at >19 mm, for extremely sensitive.
As a result, in table 1 and table 2.
MIC and the MBC(mg/mL of table 1 Chinese medicine composition to swine Escherichia coli)
Table 2 average diameter of inhibition zone (mm)
| Group | O 20 | O 161 | O 48 |
| Chinese medicine composition | 17.2 +0.13 | 16.9 +0.21 | 17.7 +0.15 |
As can be seen from Table 1 and Table 2, adopt Chinese medicine composition antibacterial effect provided by the invention better.
Embodiment 7 therapeutic tests
Culture medium
Common nutrient broth, ordinary nutrient agar, Mai Kangkai nutrient agar (extensive and profound in meaning star biotechnology responsibility Co., Ltd produces by Beijing).
Indicator
Methyl red indicator solution, alpha naphthol indicator.
Trial drug
Feed prepared by embodiment 4, gentamicine sulphate injection, 5 mL: 0. 2g (20,000 unit), by Chongqing, hundred Le Wei veterinary drug Co., Ltds produce.
Experimental animal
By test requirements document, select 280 dates of manufacture and wean (10 ~ 20 age in days) basically identical, the state of mind is good, body weight is approaching, healthy, without the DLY tri-crossbreeding of disease as experimental animal.
Reference culture
Escherichia coli reference culture O
301, from Shandong Agricultural University laboratory.
Method
Animal grouping: test pig is divided into 4 groups at random, the isolated rearing of each group, every group 70, feed prepared by the treatment by Chinese herbs group feeding embodiment of the present invention 4, be used in conjunction 3 days, gentamicin medicine group adopts parenteral solution intramuscular injection, every 1 kg body weight is injected 2 mg, 2 times on the one, be used in conjunction 3 d, positive controls, infect not administration, negative group does not infect and does not treat, gentamicin medicine group, the feed feeding that the method that positive controls and negative control group adopt embodiment 4 to prepare is prepared from, be not add in feed Chinese medicine composition additive with the difference for the treatment of by Chinese herbs group.
Escherichia coli artificial challenge and treatment situation
By large intestine
Bacillus O
301be inoculated in culture broth at desinfection chamber, adopt the advanced technology techniques such as ventilation cultivation of heating to expand numerous, bacteria containing amount is 2,000,000,000/mL ~ 5,000,000,000/mL.Chinese medicine treatment group, gentamicin medicine group, positive controls are carried out to Escherichia coli strong virus attack, and every oral 5 mL of pig, carry out artificial infection.Be used in conjunction 5d, after falling ill, carry out therapeutic test.
Curative effect judgement
Cure: after medication 3 d, pig clinical symptom disappearance, spirit recovers normal with appetite.Effective: symptom of diarrhea alleviates, appetite takes a turn for the better.Invalid: symptom of diarrhea does not alleviate or aggravates, become thin, dewater, spirit, anorexia or absolutely useless.
Test results and analysis
Escherichia coli standard serum type does slide agglutination test, before test, confirms without Escherichia coli O
301infect, after artificial infection 24 h, in the ight soil of collection, have Escherichia coli O
301, show to infect successfully.Now observe pig listlessness, appetite decline, some pigs start to have loose bowels, but are not also diarrhoea sample shape.After inoculation 36h, observe 50% pig and occur diarrhoea, ight soil is water sample shape mostly, and has indigested pellet thing.After 48 h all there is diarrhoea in Pigs Inoculated, due to individual difference order of severity difference, wherein in 60% swine excrement, has mucus or foam, now medication treatment.
Each group result for the treatment of is in table 3.
Each group of table 3 is to colibacillary clinical therapeutic efficacy
As can be seen from Table 3, the result for the treatment of of employing Chinese medicine composition will be significantly better than gentamicin medicine group.
All above-mentioned these intellectual properties of primary enforcement, do not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above, be only preferred embodiment of the present invention, is not the restriction of the present invention being made to other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (10)
1. a feed that is used for the treatment of pig enteropathogenic E．Coli disease, is characterized in that: its raw material comprises corn, wheat bran, peanut meal, the dish dregs of rice, fish meal, calcium monohydrogen phosphate, salt, methionine, threonine, vitamin and is used for the treatment of the sick Chinese medicine composition additive of pig enteropathogenic E．Coli.
2. feed as claimed in claim 1, is characterized in that: in described feed, the weight of each raw material is respectively corn 7kg～9kg, wheat bran 2kg～3kg, peanut meal 0.5kg～0.7kg, dish dregs of rice 0.8kg～1.2kg, fish meal 0.5kg～0.7kg, calcium monohydrogen phosphate 0.05kg～0.15kg, salt 0.05kg～0.15kg, methionine 0.01kg～0.03kg, threonine 0.01kg～0.03kg, vitamin 0.1kg～0.2kg, Chinese medicine composition additive 1kg～1.5kg.
3. feed as claimed in claim 1 or 2, is characterized in that: in described feed, the weight of each raw material is respectively corn 8kg, wheat bran 2.5kg, peanut meal 0.6kg, dish dregs of rice 1kg, fish meal 0.6kg, calcium monohydrogen phosphate 0.1kg, salt 0.1kg, methionine 0.02kg, threonine 0.02kg, vitamin 0.15kg, Chinese medicine composition additive 1.2kg.
4. the feed as described in claims 1 to 3, is characterized in that: the bulk drug in described Chinese medicine composition additive comprises Herba Andrographitis, the root of large-flowered skullcap, the root of herbaceous peony, cacumen biotae, leaf of negundo chastetree, the Chinese bulbul, Radix Codonopsis, radix bupleuri, madder, dark plum, Centipeda minima, hawthorn, dried orange peel, sealwort, Kaempferia galanga, caulis lonicerae, galangal, adenophora tetraphylla, Gorgon fruit, corydalis tuber, ginkgo leaf, fringed pink and radish seed.
5. the feed as described in claim 1 to 4, it is characterized in that: in described Chinese medicine composition additive, the weight of each bulk drug is respectively Herba Andrographitis 20g～30g, root of large-flowered skullcap 55g～65g, root of herbaceous peony 25g～35g, cacumen biotae 25g～35g, leaf of negundo chastetree 20g～30g, Chinese bulbul 35g～45g, Radix Codonopsis 30g～40g, radix bupleuri 20g～30g, madder 30g～40g, dark plum 25g～35g, Centipeda minima 20g～30g, hawthorn 25g～35g, dried orange peel 15g～25g, sealwort 35g～45g, Kaempferia galanga 20g～30g, caulis lonicerae 30g～40g, galangal 20g～30g, adenophora tetraphylla 25g～35g, Gorgon fruit 20g～30g, corydalis tuber 15g～25g, ginkgo leaf 30g～40g, fringed pink 25g～35g and radish seed 25g～35g.
6. a Chinese medicine composition that is used for the treatment of pig enteropathogenic E．Coli disease, is characterized in that: its bulk drug comprises Herba Andrographitis, the root of large-flowered skullcap, the root of herbaceous peony, cacumen biotae, leaf of negundo chastetree, the Chinese bulbul, Radix Codonopsis, radix bupleuri, madder, dark plum, Centipeda minima, hawthorn, dried orange peel, sealwort, Kaempferia galanga, caulis lonicerae, galangal, adenophora tetraphylla, Gorgon fruit, corydalis tuber, ginkgo leaf, fringed pink and radish seed.
7. Chinese medicine composition as claimed in claim 6, it is characterized in that: the weight of each bulk drug is respectively Herba Andrographitis 20g～30g, root of large-flowered skullcap 55g～65g, root of herbaceous peony 25g～35g, cacumen biotae 25g～35g, leaf of negundo chastetree 20g～30g, Chinese bulbul 35g～45g, Radix Codonopsis 30g～40g, radix bupleuri 20g～30g, madder 30g～40g, dark plum 25g～35g, Centipeda minima 20g～30g, hawthorn 25g～35g, dried orange peel 15g～25g, sealwort 35g～45g, Kaempferia galanga 20g～30g, caulis lonicerae 30g～40g, galangal 20g～30g, adenophora tetraphylla 25g～35g, Gorgon fruit 20g～30g, corydalis tuber 15g～25g, ginkgo leaf 30g～40g, fringed pink 25g～35g and radish seed 25g～35g.
8. the Chinese medicine composition as described in claim 6 or 7, is characterized in that: the weight of each bulk drug is respectively Herba Andrographitis 25g, root of large-flowered skullcap 60g, root of herbaceous peony 30g, cacumen biotae 30g, leaf of negundo chastetree 25g, Chinese bulbul 40g, Radix Codonopsis 35g, radix bupleuri 25g, madder 35g, dark plum 30g, Centipeda minima 25g, hawthorn 30g, dried orange peel 20g, sealwort 40g, Kaempferia galanga 25g, caulis lonicerae 35g, galangal 25g, adenophora tetraphylla 30g, Gorgon fruit 25g, corydalis tuber 20g, ginkgo leaf 35g, fringed pink 30g and radish seed 30g.
9. the preparation method of Chinese medicine composition described in claim 6 to 8, is characterized in that:
Each bulk drug component is mixed by weight, obtain mixture, add determining alcohol and be 50%～70% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 2h～3h, the amount that the mixture of every 1kg adds ethanolic solution is 3L～5L, the extract obtaining merges and filters, and leaves standstill 24h, reclaims ethanol concentrated, be condensed into thick paste, dry, pulverize into fine powder, obtain Chinese medicine composition.
10. the preparation method of feed described in claim 1 to 5, is characterized in that, comprising:
The first step, mixes the each bulk drug component in described Chinese medicine composition additive by weight, obtains mixture, add determining alcohol and be 50%～70% ethanolic solution and carry out heating and refluxing extraction 2 times, each refluxing extraction 2h～3h, the amount that the mixture of every 1kg adds ethanolic solution is 3L～5L, the extract of acquisition merges and filters, leave standstill 24h, reclaim ethanol concentrated, be condensed into thick paste, dry, be ground into fine powder, obtain described Chinese medicine composition additive;
Second step, gets the each raw material except described Chinese medicine composition additive in described feed and first pulverizes, and then the raw material after pulverizing is put into mixer and mixes, add again Chinese medicine composition additive prepared by the first step further to mix, mixed material enters modulator, and refining temperature is 70 ℃, modulating time 10 seconds, after modulation, send into slaking tank and carry out slaking, the curing time is 5 minutes, 70 ℃ of curing temperatures, finally enter pellet fabrication device and granulate, obtain finished product feedstuff.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104256096A (en) * | 2014-10-08 | 2015-01-07 | 青岛农业大学 | Feed additive and feed for treating piglet diarrhea |
| CN104757269A (en) * | 2015-03-16 | 2015-07-08 | 唐莹 | Animal colibacillosis preventing and curing traditional Chinese medicine feed additive |
| CN106212957A (en) * | 2016-08-29 | 2016-12-14 | 广西大学 | A kind of Chinese medicine feed additive preventing and treating pig bacterial disease and preparation method thereof |
| CN106721044A (en) * | 2017-03-06 | 2017-05-31 | 长沙瑞多康生物科技有限公司 | A kind of feed of prevention colibacillosis of pigs |
| CN106798128A (en) * | 2017-03-06 | 2017-06-06 | 长沙瑞多康生物科技有限公司 | A kind of feed of prevention colibacillosis of pigs |
| CN106858103A (en) * | 2017-03-06 | 2017-06-20 | 长沙瑞多康生物科技有限公司 | A kind of feed of prevention colibacillosis of pigs |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1399905A (en) * | 2002-09-03 | 2003-03-05 | 池胜碧 | Circulating utilization of pig feed and its formula |
| CN103110007A (en) * | 2013-02-01 | 2013-05-22 | 广东恒兴饲料实业股份有限公司 | Piglet compound feed composition with fermented rapeseed meal |
| CN103405536A (en) * | 2013-08-14 | 2013-11-27 | 北京元亨神农科技发展有限公司 | Chinese herbal veterinary drug used for treating diarrhoea and preparation method thereof |
-
2014
- 2014-03-06 CN CN201410078818.5A patent/CN103815134B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1399905A (en) * | 2002-09-03 | 2003-03-05 | 池胜碧 | Circulating utilization of pig feed and its formula |
| CN103110007A (en) * | 2013-02-01 | 2013-05-22 | 广东恒兴饲料实业股份有限公司 | Piglet compound feed composition with fermented rapeseed meal |
| CN103405536A (en) * | 2013-08-14 | 2013-11-27 | 北京元亨神农科技发展有限公司 | Chinese herbal veterinary drug used for treating diarrhoea and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104256096A (en) * | 2014-10-08 | 2015-01-07 | 青岛农业大学 | Feed additive and feed for treating piglet diarrhea |
| CN104757269A (en) * | 2015-03-16 | 2015-07-08 | 唐莹 | Animal colibacillosis preventing and curing traditional Chinese medicine feed additive |
| CN106212957A (en) * | 2016-08-29 | 2016-12-14 | 广西大学 | A kind of Chinese medicine feed additive preventing and treating pig bacterial disease and preparation method thereof |
| CN106721044A (en) * | 2017-03-06 | 2017-05-31 | 长沙瑞多康生物科技有限公司 | A kind of feed of prevention colibacillosis of pigs |
| CN106798128A (en) * | 2017-03-06 | 2017-06-06 | 长沙瑞多康生物科技有限公司 | A kind of feed of prevention colibacillosis of pigs |
| CN106858103A (en) * | 2017-03-06 | 2017-06-20 | 长沙瑞多康生物科技有限公司 | A kind of feed of prevention colibacillosis of pigs |
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