CN103805713A - 一种人巨细胞病毒核酸定量检测引物和探针、试剂盒及其用途 - Google Patents
一种人巨细胞病毒核酸定量检测引物和探针、试剂盒及其用途 Download PDFInfo
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- CN103805713A CN103805713A CN201410007284.7A CN201410007284A CN103805713A CN 103805713 A CN103805713 A CN 103805713A CN 201410007284 A CN201410007284 A CN 201410007284A CN 103805713 A CN103805713 A CN 103805713A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
Description
条件 | 参数 |
线性DNA折叠温度(Linear DNA folding) | 58℃ |
单价离子([Na+]) | 100mM |
双价离子([Mg++]) | 2.5mM |
线性参考品 | 质量标准(Copies/mL) | 理论浓度对数值 |
L1 | 1.0×107~9.0×107 | 7.69 |
L2 | 1.0×106~9.0×106 | 6.69 |
L3 | 1.0×105~9.0×105 | 5.69 |
L4 | 1.0×104~9.0×104 | 4.69 |
L5 | 1.0×103~9.0×103 | 3.69 |
L6 | 1.0×102~9.0×102 | 2.69 |
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CN201410007284.7A CN103805713B (zh) | 2014-01-08 | 2014-01-08 | 一种人巨细胞病毒核酸定量检测引物和探针、试剂盒及其用途 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105779644A (zh) * | 2014-12-22 | 2016-07-20 | 上海仁度生物科技有限公司 | 人巨细胞病毒的实时荧光核酸恒温扩增检测试剂盒 |
CN108130385A (zh) * | 2017-12-27 | 2018-06-08 | 上海星耀医学科技发展有限公司 | 一种人巨细胞病毒核酸检测试剂盒 |
CN110268474A (zh) * | 2017-02-08 | 2019-09-20 | 微软技术许可有限责任公司 | 用于所存储的多核苷酸的取回的引物设计 |
CN110819626A (zh) * | 2019-11-29 | 2020-02-21 | 上海之江生物科技股份有限公司 | 一种磁珠法hcmv核酸提取用裂解液及其应用 |
CN114622040A (zh) * | 2022-04-22 | 2022-06-14 | 华中科技大学同济医学院附属同济医院 | 检测粪便中人巨细胞病毒的pcr引物组及其微滴式数字pcr试剂盒 |
US11783918B2 (en) | 2016-11-30 | 2023-10-10 | Microsoft Technology Licensing, Llc | DNA random access storage system via ligation |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101397591A (zh) * | 2008-10-30 | 2009-04-01 | 浙江大学 | 实时荧光定量PCR检测HCMV gBn的分型方法 |
CN101760560A (zh) * | 2008-10-23 | 2010-06-30 | 上海复星医药(集团)股份有限公司 | 一种人巨细胞病毒(hcmv)荧光pcr检测方法 |
US20130157253A1 (en) * | 2011-12-16 | 2013-06-20 | Mei Wang Baker | Detection of cytomegalovirus dna using amplification from blood samples |
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2014
- 2014-01-08 CN CN201410007284.7A patent/CN103805713B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101760560A (zh) * | 2008-10-23 | 2010-06-30 | 上海复星医药(集团)股份有限公司 | 一种人巨细胞病毒(hcmv)荧光pcr检测方法 |
CN101397591A (zh) * | 2008-10-30 | 2009-04-01 | 浙江大学 | 实时荧光定量PCR检测HCMV gBn的分型方法 |
US20130157253A1 (en) * | 2011-12-16 | 2013-06-20 | Mei Wang Baker | Detection of cytomegalovirus dna using amplification from blood samples |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105779644A (zh) * | 2014-12-22 | 2016-07-20 | 上海仁度生物科技有限公司 | 人巨细胞病毒的实时荧光核酸恒温扩增检测试剂盒 |
CN105779644B (zh) * | 2014-12-22 | 2019-10-11 | 上海仁度生物科技有限公司 | 人巨细胞病毒的实时荧光核酸恒温扩增检测试剂盒 |
US11783918B2 (en) | 2016-11-30 | 2023-10-10 | Microsoft Technology Licensing, Llc | DNA random access storage system via ligation |
CN110268474A (zh) * | 2017-02-08 | 2019-09-20 | 微软技术许可有限责任公司 | 用于所存储的多核苷酸的取回的引物设计 |
CN110268474B (zh) * | 2017-02-08 | 2023-03-31 | 微软技术许可有限责任公司 | 用于所存储的多核苷酸的取回的引物设计 |
CN108130385A (zh) * | 2017-12-27 | 2018-06-08 | 上海星耀医学科技发展有限公司 | 一种人巨细胞病毒核酸检测试剂盒 |
CN110819626A (zh) * | 2019-11-29 | 2020-02-21 | 上海之江生物科技股份有限公司 | 一种磁珠法hcmv核酸提取用裂解液及其应用 |
CN110819626B (zh) * | 2019-11-29 | 2021-11-26 | 上海之江生物科技股份有限公司 | 一种磁珠法hcmv核酸提取用裂解液及其应用 |
CN114622040A (zh) * | 2022-04-22 | 2022-06-14 | 华中科技大学同济医学院附属同济医院 | 检测粪便中人巨细胞病毒的pcr引物组及其微滴式数字pcr试剂盒 |
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