CN103783044B - The using method of chitin oligosaccharide in arviculture - Google Patents
The using method of chitin oligosaccharide in arviculture Download PDFInfo
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- CN103783044B CN103783044B CN201410065507.5A CN201410065507A CN103783044B CN 103783044 B CN103783044 B CN 103783044B CN 201410065507 A CN201410065507 A CN 201410065507A CN 103783044 B CN103783044 B CN 103783044B
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Abstract
The using method of chitin oligosaccharide provided by the invention in arviculture, is first dissolved as solution by chitin oligosaccharide with water, then cloth imposes on crops as foliage fertilizer; The mean molecule quantity of the chitin oligosaccharide that is used as is 500-20000, and chitin oligosaccharide solution concentration is 0.3-3g/L, and spraying leaf number of times whole growth period is 3-5 times. First chitin oligosaccharide is carried out to solid-state or liquid dilution before use foliage fertilizer after, cloth imposes on crops as base manure; The mean molecule quantity of chitin oligosaccharide used is 1000-30000, and the consumption of chitin oligosaccharide is 100-2000 grams/acre. Compared with prior art, the inventive method is simple, has given full play to chitin oligosaccharide in the biochemical functions of activated plant growth and has prevented the effect aspect disease. After application, under the prerequisite of use amount that reduces chemical fertilizer, agricultural chemicals, maintain or improve the output of crops.
Description
Technical field
What the present invention relates to is the using method of a kind of chitin oligosaccharide in arviculture, relates to specifically oneCultivating agent taking marine bioactivity material chitin oligosaccharide (chitosan oligosaccharide) as crops, this cultivating agent has antibacterial, growth promotionDeng effect, can reduce agricultural chemicals and fertilizer amount, improve crop quality and suitable improving yield.
Background technology
Shitosan, chitosan oligosaccharide are owing to having amino in molecule, therefore its aqueous solution become the structures such as positively charged colloidFeature, animals and plants life entity is there is to the effects such as multiple physiology adjusting, shitosan and chitosan oligosaccharide are become cross-centennial the heaviestThe marine active material of wanting.
Shitosan (chitosan, poly-Isosorbide-5-Nitrae-β-gucosamine) is the chain that 1000-3000 gucosamines linkHigh polymer, its chemical formula is (C6H11O4N) n, structural formula is:
Shitosan is the deep processed product of chitin (chitin). Chitin is extensively present in the crust of crab, shrimp etc., itsDe-acetyl product claims shitosan. Shitosan is described as Europe, the United States, day the beyond sugar, fat, proteins,vitamins,and mineralsSix vital principles. The 6th vital principle is different from general bioactivator completely, is that human body cell is requisite importantPart, has reinforced immunological power, suppresses aging, prevent disease, promotion disease recovery from illness and regulate human physiological functions etc. manyPlant function. But because chitosan molecule amount is up to hundreds thousand of, poorly water-soluble, has limited application.
When n < 25-30, i.e. mean molecule quantity < 6000 o'clock, are called chitin oligosaccharide or chitosan oligosaccharide (chitosanoligoSaccharide). Chitosan oligosaccharide (chitin oligosaccharide), as the depression of order product good water solubility of shitosan, has been widely used in medicine and has protectedThe fields such as strong product. According to the study, chitosan oligosaccharide, in growing process, also has good effect. The mechanism of action difference of chitosan oligosaccharideIn conventional fertilizers, it produces resistance protein phytoalexin, chitinase, chitosan enzyme etc. by inducing plant, effectively anti-The disease that only fungi, bacterium, virus cause. Can also activated plant the biochemical functions of growth, improve the emergence rate that sprouts, promotePlant growth improves gourd, fruit and vegetable taste.
The beginning of this century, China has carried out the developmental research of chitin oligosaccharide as arviculture agent, but content is equalBe limited to manufacture and the formula of fertilizer or bactericide, and chitin oligosaccharide in fertilizer or bactericide as a component or carrierExist. For example Chinese invention patent ZL200410035619.2 " preparation method of chitin oligosaccharide zinc borone magnesium fertilizer " is " by chitinOr the acid-soluble solution of shitosan, temperature control, adds the strand of hydrogen peroxide or enzyme brisement chitin or chitin, and degraded 0.5-12 is littleTime, filter, add amino acid, zinc sulfate, magnesium sulfate and boric acid ... " Chinese invention patent ZL200410041695.4 and for example" compound chitooligosaccharidefoliar foliar fertilizer " " formed by following composition by weight: chitin oligosaccharide 1.0-4.0%, compound amino acid 10-20%, urea 15-30%, containing micro-compound 10-30% ... " Chinese invention patent for another exampleZL200410050480.9 " a kind of preparation method of novel low toxicity crop bactericide " " taking high molecular weight chitosan as raw material,Between its molecular weight 50 ten thousand-100 ten thousand, deacetylation is 65-100%, and in homogeneous solvent, shitosan adopts microwave skillArt is carried out degradation reaction, and in degradation solution, directly adds metallic compound to carry out complexation reaction ... obtain chitin oligosaccharideMetal complex " " chitin oligosaccharide metal complex has very strong inhibitory action to pathogen of crop ".
Due to processing method complexity, and be mostly compound or mixture, cause high expensive.
Summary of the invention
For above-mentioned deficiency, technical problem to be solved by this invention adopts one pack system chitin oligosaccharide as farming exactlyThing cultivating agent, proposes the using method of a kind of chitin oligosaccharide in arviculture.
The using method of chitin oligosaccharide provided by the invention in arviculture, first by chitin oligosaccharide with water-soluble solutionFor solution, then cloth imposes on crops as foliage fertilizer; The mean molecule quantity of the chitin oligosaccharide that is used as is 500-20000, crustOligosaccharide solution concentration is 0.3-3g/L, and spraying leaf number of times whole growth period is 3-5 times. First that crust is low before use foliage fertilizerAfter glycan carries out solid-state or liquid dilution, cloth imposes on crops as base manure, and wherein solid-state dilution is to mix with other fertilizer or soilWith, liquid dilution is to be dissolved as solution with water; The mean molecule quantity of chitin oligosaccharide used is 1000-30000, and crust is oligomericThe consumption of sugar is 100-2000 grams/acre.
During as foliage fertilizer, the mean molecule quantity of chitin oligosaccharide is 3000, and concentration is 0.5g/L.
During as base manure, the mean molecule quantity of chitin oligosaccharide is 7000, and consumption is 500g/ mu.
The using method of chitin oligosaccharide provided by the invention in arviculture, using chitin oligosaccharide as single groupPoint, after dilution directly or impose on crops as base manure or foliage fertilizer by the indirect cloth of soil. Compared with prior art, thisBright method is simple, has given full play to the biochemical functions of chitin oligosaccharide in activated plant growth. After application, reducingUnder the prerequisite of the use amount of chemical fertilizer, agricultural chemicals, maintain or improve the output of crops.
Detailed description of the invention
(1) celery, green vegetables, Mao Maocai:
Being averaged molecular weight and being 200 grams, 3000 chitin oligosaccharide pulvis mixes thoroughly and imposes on 1 mu of capable sowing of Tian Shangzai with soil.
Be averaged molecular weight and be the aqueous solution that 1000 chitin oligosaccharide is mixed with 0.2g/L, at the interim spray leaf of vegetable growth2 times.
Result shows: compared with not carrying out the growing vegetables of the same race of aforesaid operations, the pesticide carbendazim of use is by every mu 100Gram be kept to 30 grams, Günther Heidemann composite fertilizer reduces to 15 kilograms by 30 kilograms every mu, and yield of vegetables is substantially identical.
(2) cucumber, tomato
700 grams/acre of chitin oligosaccharides that is 5000 with mean molecule quantity are base manure.
In growth period, spray leaf 3 times taking mean molecule quantity as 3000 the chitin oligosaccharide aqueous solution, the 1st time concentration is 0.3g/L, the 2nd, 3 times is 2g/L.
Result shows: compared with not carrying out the growing vegetables of the same race of aforesaid operations, and volume increase 10%, mouthfeel is better.
(3) tealeaves
Be made into the aqueous solution of 0.1g/L taking mean molecule quantity as 7000 chitin oligosaccharide, oligomeric by every mu of ground 1kg crustThe consumption of Icing Sugar agent is filled with root to tea tree.
After sprouting to pluck before be made into taking mean molecule quantity as 1500 chitin oligosaccharide 1g/L concentration the aqueous solution spray leaf 3Inferior.
Result shows: compared with not carrying out the Tea planting of aforesaid operations, tealeaves winter resistance improves, and Tea Polyphenols increases by 10%Above, bud ratio improves, volume increase 15%.
(4) Chinese medicine corydalis tuber, the stem of noble dendrobium
Chitin oligosaccharide pulvis taking mean molecule quantity as 10000 by every mu of 1.2kg as base manure.
The chitin oligosaccharide aqueous solution spray leaf of crop growth period taking mean molecule quantity as 1,500 5 times, concentration is controlled at 0.8-1g/L。
Result shows: compared with not carrying out the medicinal material plantation of the same race of aforesaid operations, and volume increase 10%.
(5) paddy rice
In growth period, spray leaf 5 times taking mean molecule quantity as 2000 the chitin oligosaccharide aqueous solution, concentration is for first 2 times0.3g/L, rear is for 3 times 8g/L.
Result shows: compared with not carrying out the Rice Cropping of aforesaid operations, pesticide dosage reduces half, increasing yield of paddy 3%.
(6) corn
Apply base manure taking mean molecule quantity as 30000 chitin oligosaccharide pulvis by 2kg/ mu.
In growth period, spray leaf 4 times, the 1st, 2 chitin oligosaccharide aqueous solution that are 1000 with mean molecule quantity, concentration is0.08g/L, the 3rd, 4 chitin oligosaccharide aqueous solution that are 20000 with mean molecule quantity, concentration is 3g/L.
Result shows: compared with not carrying out the corn planting of aforesaid operations, and volume increase 4%.
Claims (3)
1. the using method of chitin oligosaccharide in arviculture, is first dissolved as solution by chitin oligosaccharide with water, thenCloth imposes on crops as foliage fertilizer; The mean molecule quantity of the chitin oligosaccharide that is used as is 500-20000, and chitin oligosaccharide is moltenLiquid concentration is 0.3-3g/L, and spraying leaf number of times whole growth period is 3-5 times; It is characterized in that before use foliage fertilizer first that crust is lowAfter glycan carries out solid-state or liquid dilution, cloth imposes on crops as base manure, and wherein solid-state dilution is to mix with other fertilizer or soilWith, liquid dilution is to be dissolved as solution with water; The mean molecule quantity of chitin oligosaccharide used is 1000-30000, and crust is oligomericThe consumption of sugar is 100-2000 grams/acre.
2. the using method of chitin oligosaccharide as claimed in claim 1 in arviculture, is characterized in that as foliage fertilizerTime chitin oligosaccharide mean molecule quantity be 3000, concentration is 0.5g/L.
3. the using method of chitin oligosaccharide as claimed in claim 1 or 2 in arviculture, is characterized in that as base manureTime chitin oligosaccharide mean molecule quantity be 7000, consumption is 500g/ mu.
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| CN201410065507.5A CN103783044B (en) | 2014-02-26 | 2014-02-26 | The using method of chitin oligosaccharide in arviculture |
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| CN201410065507.5A CN103783044B (en) | 2014-02-26 | 2014-02-26 | The using method of chitin oligosaccharide in arviculture |
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| CN103783044A CN103783044A (en) | 2014-05-14 |
| CN103783044B true CN103783044B (en) | 2016-05-18 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106171453B (en) * | 2016-07-25 | 2019-04-05 | 郑婵颖 | Absorption method for determination of amount of the crops to chitosan oligosaccharide |
| CN106957201A (en) * | 2017-05-08 | 2017-07-18 | 安徽新荣久农业科技有限公司 | Tea oil tree special-purpose foliage fertilizer and preparation method thereof |
| CN109717015A (en) * | 2018-12-27 | 2019-05-07 | 中科荣耀(苏州)生物科技有限公司 | One kind, which has, promotes the active low deacetylation chitosan oligosaccharide of plant growth and its application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101543230A (en) * | 2008-03-28 | 2009-09-30 | 中国科学院大连化学物理研究所 | Plant stress-resistant inducer and application thereof |
| US20100093537A1 (en) * | 2007-01-09 | 2010-04-15 | Emd Crop Bioscience, Inc. | Lipo-chitooligosaccharide combination compositions for enhanced plant growth and yield |
| CN102144637A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102415384A (en) * | 2011-10-28 | 2012-04-18 | 海南正业中农高科股份有限公司 | Purpose of chitosan oligosaccharide for regulating crop growth |
| CN102523955A (en) * | 2010-12-28 | 2012-07-04 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide aqueous solution to improve crop quality and usage of chitosan oligosaccharide aqueous solution |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430944B (en) * | 2013-09-15 | 2015-06-10 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide-containing composition to promotion of swelling of subterraneous stems and tuberous roots of plants |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100093537A1 (en) * | 2007-01-09 | 2010-04-15 | Emd Crop Bioscience, Inc. | Lipo-chitooligosaccharide combination compositions for enhanced plant growth and yield |
| CN101543230A (en) * | 2008-03-28 | 2009-09-30 | 中国科学院大连化学物理研究所 | Plant stress-resistant inducer and application thereof |
| CN102144637A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102523955A (en) * | 2010-12-28 | 2012-07-04 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide aqueous solution to improve crop quality and usage of chitosan oligosaccharide aqueous solution |
| CN102415384A (en) * | 2011-10-28 | 2012-04-18 | 海南正业中农高科股份有限公司 | Purpose of chitosan oligosaccharide for regulating crop growth |
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