CN103782809B - Ultra-thin interstock grafting method - Google Patents

Ultra-thin interstock grafting method Download PDF

Info

Publication number
CN103782809B
CN103782809B CN201410050828.8A CN201410050828A CN103782809B CN 103782809 B CN103782809 B CN 103782809B CN 201410050828 A CN201410050828 A CN 201410050828A CN 103782809 B CN103782809 B CN 103782809B
Authority
CN
China
Prior art keywords
interstock
grafting
ultra
thin
grafting method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201410050828.8A
Other languages
Chinese (zh)
Other versions
CN103782809A (en
Inventor
詹志翔
许梅花
马瑞红
陈翠
赵铮
陈春
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CHENGJIANG COUNTY ECONOMY CROP WORK STATION
Original Assignee
CHENGJIANG COUNTY ECONOMY CROP WORK STATION
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CHENGJIANG COUNTY ECONOMY CROP WORK STATION filed Critical CHENGJIANG COUNTY ECONOMY CROP WORK STATION
Priority to CN201410050828.8A priority Critical patent/CN103782809B/en
Publication of CN103782809A publication Critical patent/CN103782809A/en
Application granted granted Critical
Publication of CN103782809B publication Critical patent/CN103782809B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to a grafting method, and belongs to the technical field of cultivation of plants. According to the ultra-thin interstock grafting method, the interstocks are needed for survival or the economical plants can be achieved through the interstocks. The ultra-thin interstock grafting method includes the following steps that firstly, rootstocks are selected; second, the interstocks are selected and processed; third, scions are selected; fourth, grating is performed. According to the technical scheme, the grafting field can be expanded, the expanding propogation coefficient is increased, the growth vigor of plants is adjusted, the stress resistance is enhanced, the seedling using cost is effectively reduced, the grating survival rate is high, the method is easy and convenient to perform, and high popularization and application value is achieved.

Description

Ultra-thin interstock grafting method
Technical field
The present invention relates to a kind of grafting method, need interstock just can survive or need interstock just can reach the grafting method of economic characters plant, belong to field of plant growing technology.
Background technology
Fruit tree, vegetable grafting culture technique, be mainly used in increasing and expand numerous coefficient, and adjustment plant strain growth gesture, strengthens resistance, improve the yield and quality.For many years, oneself obtains the application of wide model in production for fruit tree, vegetable grafting cultivation, and really, vegetables industry obtains tremendous development, for solid technological basis has been laid in growth of agricultural efficiency, increasing peasant income.But some plant is just difficult to find the stock needing proterties, have affinity, there is certain drawback in conventional interstock grafting, as: stock affinity is inadequate, resistance is poor, breeding is difficult, interstock grafting is slow, high in cost of production series of problems, limit some fruits, vegetables industrial benefit space, even form technology bottle stem and hinder some fruits, vegetables industry development.
Such as: citrus interstock once-seedling forming grafting--on the basis of interstock grafting, again scion is grafted on interstock simultaneously, once complete grafting operation, accomplish that base anvil and interstock, scion and interstock, scion and interstock heal, once-seedling forming simultaneously.But this method conducting system is not formed, the easy dehydration of secondary grafting scion is dead, and therefore survival rate is extremely low; Moreover this method is in fact also secondary grafting, fail to raise the efficiency, reduce costs.
Summary of the invention
For expanding grafting field, shortening the seedling time, reducing grafting cost.The invention provides a kind of ultra-thin interstock grafting method, to reduce input, increase the benefit, for useful path is added in increasing peasant income again.
The present invention is completed by following technical proposal:
Ultra-thin interstock grafting method, through the following step:
A, select stock: the plant selecting easily breeding, strong stress resistance, growth potential good and strong with interstock affinity makes stock;
B, selection interstock and process: select there is the plant of better affinity as interstock with stock and scion, adopt growth vigorous shoot tip meristem desinfection chamber clear water rinsed clean or with banister brush by dust outwash, more the washing with soap lye of fine hair, and then wash away soap lye with clear water, wash rear blotting paper and blot surface moisture, with 70% alcohol leaching several seconds, with 10% calcium hypochlorite saturated supernatant vacuole 10-20 minute after taking out, or divide kind with 2%-10% this sodium solution of chlorine immersion 6-15, aseptic water washing 3 times are used after sterilization, with sterile gauze or aseptic paper suck dry moisture, smash taking juice to pieces, add NAA0.3mg/L, 6-BA0.5mg/L is for subsequent use as ultra-thin interstock, now-making-now-using,
C, selection scion: select to have with interstock to do scion compared with the plant improved seeds of strong affinity;
D, grafting: adopt the conventional grafting method being suitable for institute's graft, ultra-thin interstock is dipped in scion, alignment forms layer and inserts, with film bundling.
Interstock plant in described step B, the tender tip of non-lignification, guarantees to survive.
The grafting method that in described D step, conventional grafting method is suitable for the most for institute's grafting plant.
The present invention has following advantages and effect: adopt technique scheme, can expand grafting field, increases and expands numerous coefficient, adjustment plant strain growth gesture, strengthens resistance, effectively reduces and uses seedling cost, grafting survival is high, simple to operation, has higher application value.
Specific embodiment
embodiment 1
Ultra-thin interstock grafting method, through the following step:
A, selection stock: select high macrospecies climing holder trailing plants to make stock;
B, selection interstock and process: select annual climing holder trailing plants as interstock; Adopt the vigorous shoot tip meristem of growth at desinfection chamber clear water rinsed clean, more the washing with soap lye of fine hair, and then wash away soap lye with clear water, wash rear blotting paper and blot surface moisture, with 70% alcohol leaching several seconds, with 10% calcium hypochlorite saturated supernatant vacuole 10-20 minute after taking out, use aseptic water washing 3 times after sterilization, use sterile gauze suck dry moisture, smash taking juice to pieces, add NAA0.3mg/L, 6-BA0.5mg/L for subsequent use as ultra-thin interstock, now-making-now-using;
C, selection scion: select tomato to do scion;
D, grafting: adopt the conventional grafting method being suitable for institute's graft, ultra-thin interstock is dipped in scion, alignment forms layer and inserts, with film bundling.
Interstock plant in described step B, the tender tip of non-lignification, guarantees to survive.
The grafting method that in described D step, conventional grafting method is suitable for the most for institute's grafting plant.
High macrospecies climing holder trailing plants be perennial, tree height is large, growth potential is prosperous, strong stress resistance and solanaceous vegetables grafting affinity not, not easily survive during grafting; Medium and the high macrospecies of annual climing holder trailing plants growth potential, resistance is climing asks trailing plants, Tomato Grafting affinity all high.Realizing stronger amount of growth, comparatively high resistance to cold and diseases adopts conventional grafting method to complete, adopt interstock grafting two months ability seedlings, seedling raise period is long, cost is high, adopt this law grafting, within 20 days, emerge, only be equivalent to the cost of conventional grafting, survival rate more than 90% is suitable with conventional grafting, and grafting growth is vigorous, strong stress resistance, output are high.
embodiment 2
Ultra-thin interstock grafting method, through the following step:
A, selection stock: select high macrospecies climing holder trailing plants to make stock;
B, selection interstock and process: select annual climing holder trailing plants as interstock; Adopt the vigorous shoot tip meristem of growth at desinfection chamber clear water rinsed clean, more the washing with soap lye of fine hair, and then wash away soap lye with clear water, wash rear blotting paper and blot surface moisture, with 70% alcohol leaching several seconds, soak 6-15 with 2% this sodium solution of chlorine after taking out and divide kind, use aseptic water washing 3 times after sterilization, use sterile gauze suck dry moisture, smash taking juice to pieces, add NAA0.3mg/L, 6-BA0.5mg/L for subsequent use as ultra-thin interstock, now-making-now-using;
C, selection scion: select eggplant to do scion;
D, grafting: adopt the conventional grafting method being suitable for institute's graft, ultra-thin interstock is dipped in scion, alignment forms layer and inserts, with film bundling.
Interstock plant in described step B, the tender tip of non-lignification, guarantees to survive.
The grafting method that in described D step, conventional grafting method is suitable for the most for institute's grafting plant.
High macrospecies climing holder trailing plants be perennial, tree height is large, growth potential is prosperous, strong stress resistance and solanaceous vegetables grafting affinity not, not easily survive during grafting; Medium and the high macrospecies of annual climing holder trailing plants growth potential, resistance is climing asks trailing plants, Grafted Eggplant affinity all high.Realizing stronger amount of growth, comparatively high resistance to cold and diseases adopts conventional grafting method to complete, adopt interstock grafting two months ability seedlings, seedling raise period is long, cost is high, adopt this law grafting, within 20 days, emerge, only be equivalent to the cost of conventional grafting, survival rate more than 90% is suitable with conventional grafting, and grafting growth is vigorous, strong stress resistance, output are high.
embodiment 3
Ultra-thin interstock grafting method, through the following step:
A, selection stock: select high macrospecies climing holder trailing plants to make stock;
B, selection interstock and process: select annual climing holder trailing plants as interstock; Adopt growth vigorous shoot tip meristem desinfection chamber with banister brush by dust outwash, more the washing with soap lye of fine hair, and then wash away soap lye with clear water, wash rear blotting paper and blot surface moisture, with 70% alcohol leaching several seconds, kind is divided with soaking 6-15 with 10% this sodium solution of chlorine after taking out, use aseptic water washing 3 times after sterilization, use aseptic paper suck dry moisture, smash taking juice to pieces, add NAA0.3mg/L, 6-BA0.5mg/L for subsequent use as ultra-thin interstock, now-making-now-using;
C, selection scion: select capsicum to do scion;
D, grafting: adopt the conventional grafting method being suitable for institute's graft, ultra-thin interstock is dipped in scion, alignment forms layer and inserts, with film bundling.
Interstock plant in described step B, the tender tip of non-lignification, guarantees to survive.
The grafting method that in described D step, conventional grafting method is suitable for the most for institute's grafting plant.
High macrospecies climing holder trailing plants be perennial, tree height is large, growth potential is prosperous, strong stress resistance and solanaceous vegetables grafting affinity not, not easily survive during grafting; Medium and the high macrospecies of annual climing holder trailing plants growth potential, resistance is climing asks trailing plants, capsicum grafting affinity all high.Realizing stronger amount of growth, comparatively high resistance to cold and diseases adopts conventional grafting method to complete, adopt interstock grafting two months ability seedlings, seedling raise period is long, cost is high, adopt this law grafting, within 20 days, emerge, only be equivalent to the cost of conventional grafting, survival rate more than 90% is suitable with conventional grafting, and grafting growth is vigorous, strong stress resistance, output are high.

Claims (3)

1. ultra-thin interstock grafting method, is characterized in that, through the following step:
A, select stock: the plant selecting easily breeding, strong stress resistance, growth potential good and strong with interstock affinity makes stock;
B, selection interstock and process: select there is the plant of better affinity as interstock with stock and scion, adopt growth vigorous shoot tip meristem desinfection chamber clear water rinsed clean or with banister brush by dust outwash, more the washing with soap lye of fine hair, and then wash away soap lye with clear water, wash rear blotting paper and blot surface moisture, with 70% alcohol leaching several seconds, with 10% calcium hypochlorite saturated supernatant vacuole 10-20 minute after taking out, or divide kind with 2%-10% this sodium solution of chlorine immersion 6-15, aseptic water washing 3 times are used after sterilization, with sterile gauze or aseptic paper suck dry moisture, smash taking juice to pieces, add NAA0.3mg/L, 6-BA0.5mg/L is for subsequent use as ultra-thin interstock, now-making-now-using,
C, selection scion: select to have with interstock to do scion compared with the plant improved seeds of strong affinity;
D, grafting: adopt the conventional grafting method being suitable for institute's graft, ultra-thin interstock is dipped in scion, alignment forms layer and inserts, with film bundling.
2. ultra-thin interstock grafting method according to claim 1, is characterized in that, interstock plant in described step B, and the tender tip of non-lignification, guarantees to survive.
3. ultra-thin interstock grafting method according to claim 1 and 2, is characterized in that, the grafting method that in described D step, conventional grafting method is suitable for the most for institute's grafting plant.
CN201410050828.8A 2014-02-14 2014-02-14 Ultra-thin interstock grafting method Expired - Fee Related CN103782809B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410050828.8A CN103782809B (en) 2014-02-14 2014-02-14 Ultra-thin interstock grafting method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410050828.8A CN103782809B (en) 2014-02-14 2014-02-14 Ultra-thin interstock grafting method

Publications (2)

Publication Number Publication Date
CN103782809A CN103782809A (en) 2014-05-14
CN103782809B true CN103782809B (en) 2015-06-17

Family

ID=50659198

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410050828.8A Expired - Fee Related CN103782809B (en) 2014-02-14 2014-02-14 Ultra-thin interstock grafting method

Country Status (1)

Country Link
CN (1) CN103782809B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6222720B2 (en) * 2014-10-17 2017-11-01 国立大学法人名古屋大学 Grafting plant body and production method thereof
CN105265192B (en) * 2015-11-19 2018-01-09 北京农学院 A kind of cotton grafting method of high-survival rate
CN105766386B (en) * 2016-03-09 2019-03-12 黔东南苗族侗族自治州农业科学院 A kind of method of pomelo shaddock intermediate stock control dehiscent fruit
CN116267272A (en) * 2021-12-03 2023-06-23 宁夏天瑞绿色种业有限公司 Grafting method and application of pepper seedlings

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1926949A (en) * 2006-09-27 2007-03-14 贵州宏晨山茶花资源开发研究所 Method for grafting various camellias on a big tree
CN103314769A (en) * 2013-07-16 2013-09-25 李雷 Spiral grafting method of homophytic multi-strain camellias on same branch
CN103314768A (en) * 2013-07-16 2013-09-25 李雷 Camellia grafting method by utilizing buxus sinica stock
CN103385116A (en) * 2013-07-16 2013-11-13 李雷 Method for grafting multi-strain tea flowers on same rootstock

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1926949A (en) * 2006-09-27 2007-03-14 贵州宏晨山茶花资源开发研究所 Method for grafting various camellias on a big tree
CN103314769A (en) * 2013-07-16 2013-09-25 李雷 Spiral grafting method of homophytic multi-strain camellias on same branch
CN103314768A (en) * 2013-07-16 2013-09-25 李雷 Camellia grafting method by utilizing buxus sinica stock
CN103385116A (en) * 2013-07-16 2013-11-13 李雷 Method for grafting multi-strain tea flowers on same rootstock

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《红星苹果矮化中间砧试验研究》;洪建源等;《沈阳农业大学学报》;19890402;第1-8页 *
《葡萄中间砧对生长结果影响的研究》;王美军等;《中外葡萄与葡萄酒》;20090515;第33-34页 *
《黄瓜砧木断根插接育苗技术研究》;马红等;《山东农业大学学报》;19970630;第121-126页 *

Also Published As

Publication number Publication date
CN103782809A (en) 2014-05-14

Similar Documents

Publication Publication Date Title
CN103858770B (en) A kind of method of floral leaf jade hairpin Fast-propagation
CN103782809B (en) Ultra-thin interstock grafting method
CN102405843B (en) Seedling method for tissue culture and rapid breeding of camellia semiserrata chi
CN103988776B (en) A kind of Nantong little side's persimmon tissue culture and rapid propagation method
CN105191792B (en) The rapid propagation method of almond ringdove chrysanthemum
CN104938328B (en) A kind of selection of anti-root rot sweet potato variety
CN106561375A (en) Peach tree cuttage seedling-raising method
CN108094210A (en) A kind of cultural method of dragon fruit plant
CN105432327A (en) Method for preventing fruit cracking disease of citrus grandis osbeck
CN102696482B (en) Method for detoxifying clonal tissue culture explant of Camellia oleifera Abel
Rahimi et al. Study on effects of different Plant Growth Regulators types in shoot regeneration and node formation of Sutsuki Azalea (Rhododendron indicum): a commercially important bonsai
CN103070072A (en) Method for fast propagation of test tube water chestnut by tissue culture
CN103798138B (en) A kind of is the method that outer implant sets up Bulbus Lilii embryo callus subculture regenerating system with gynoecium
CN105145354A (en) Method for aseptically seeding ebony and establishing leaf-cutting rapid propagation system
CN106973693B (en) Method for rapidly recovering fertility of lily distant hybrid
CN108552059B (en) Plant tissue culture method for promoting strong roots of potato seedlings
Rizwan et al. Effect of different pretreatments on breaking seed dormancy and in Vitro germination in Jatropha Curcas L
CN111406647A (en) Efficient starting culture medium for directly inducing tetraploid paulownia petioles to regenerate adventitious buds and application
CN105028188A (en) Method for breeding early-ripe apples by using bud mutation
Mbinda et al. Efficient plant regeneration of selected Kenyan sweetpotato (Ipomoea batatas (L.) Lam.) cultivars through somatic embryogenesis
Berhanu et al. Factors Affecting in Vitro Propagation of Cassava (Manihot Esculenta Crantz.) Euphorbiaceae, Varieties of ‘Kello’and ‘Qulle
CN104756870A (en) Tissue culture propagation method of parashorea chinensis germinated seeds
CN104221882B (en) Explant sterilization method for ginkgo biloba tissue culture
Hossain et al. In vitro propagation of the giant bamboo Dendrocalamus giganteus Munro
CN104186315B (en) Rapidly and efficiently carnation Regeneration System method

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150617

Termination date: 20160214

CF01 Termination of patent right due to non-payment of annual fee