CN106973693B - Method for rapidly recovering fertility of lily distant hybrid - Google Patents

Abstract

The invention discloses a method for rapidly recovering fertility of lily distant hybrids, which comprises the steps of covering buds of the shoot tips of the flowering distant lilies with an amoulam solution; comprises the steps of treating flower buds of distant hybrid lily with an amosulalin solution and then washing with water. The method can obtain fertile distant lily germplasm.

Description

Method for rapidly recovering fertility of lily distant hybrid

Technical Field

The invention relates to the technical field of control for restoring fertility of lily distant hybrids, in particular to a method for quickly restoring fertility of lily distant hybrids.

Background

The lily (L ilium) is a general name of all species of lilies, has a colorful and unique flower appearance, is one of world famous flowers, is a top-grade famous and precious flower in the reputation global market, is widely applied in gardens, pot flowers, particularly fresh cut flowers, and the like, is deeply favored by people, western people always take the lily as a holy and clean symbol, China always uses lily to represent a pure and happy custom, is originated from luck connotation of lily in the world, is originated from Luck living matters of Shabai-Shu-Hao-Hai-Wu-Hai-Luo-Lu-a, and-Lu.

Disclosure of Invention

The present invention aims to provide a method for rapidly restoring fertility to lily distant hybrids, which solves the problems of the prior art as noted in the background.

The invention is realized by the following steps:

the invention relates to a method for rapidly recovering fertility of lily distant hybrid, which comprises the steps of covering buds of the shoot tips of the flowering distant lily bulbs with an amoulam solution; comprises the steps of treating flower buds of distant hybrid lily with an amosulalin solution and then washing with water.

Wherein the concentration of the adopted oryzalin solution is 4-10 mu M. The time for treating the flower buds of the distant hybrid lily is 2-3 days; the number of washing with water was 5.

Specifically, the method comprises the following steps:

1) cultivating distant lilies: selecting healthy distant lilies without diseases and insect pests, and carrying out lily cultivation management according to a conventional method, wherein the circumference of the bulb is larger than 12 cm;

2) and (3) lily bud treatment: selecting flowering distant lily bulbs, cultivating by a conventional method, dipping 4-10 mu M sulfamonomethoxine solution into cotton balls when flower buds appear, covering the flower buds on stem tips, treating flower buds of the distant lily bulbs for 2-3 days by 4-10 mu M sulfamonomethoxine, and washing for 5 times by water;

3) and (3) fertility restoration identification: observing the fertile pollen grains obtained after fertility restoration under a microscope, observing pollen germination and forming a pollen tube under the microscope, and pollinating the pollen on the parent lily variety to generate a filial generation; meanwhile, the female gamete can be fertile, and can also bear fruits after pollination to generate filial generation;

4) breeding of filial generation: after the distant hybrid and the parent variety are hybridized and fructified, the hybrid plant can be obtained by adopting the conventional bead matching culture and embryo culture method, and an excellent new lily distant hybrid variety is bred from the hybrid plant.

The most key point of the invention is that cotton balls are dipped in 4-10 mu M oryzalin solution to cover buds at stem tips, the oryzalin solution with the concentration of 4-10 mu M is adopted to treat flower buds of distant hybrid lily for 2-3 days, and then the flower buds are washed by water for 5 times. Thus, fertile lilium distans germplasm can be obtained.

Detailed Description

The present invention will be described in further detail with reference to examples.

Example 1:

1) cultivating distant lilies: selecting healthy distant lily seeds without diseases and insect pests, and carrying out lily cultivation management according to a conventional method, wherein the circumference of each seed ball is larger than 12 cm;

2) and (3) lily bud treatment: selecting flowering distant lily bulbs, cultivating by a conventional method, dipping 4-10 mu M sulfamonomethoxine solution on the buds of the stem tips when the buds appear, treating the buds of the distant lily bulbs for 2-3 days by 4-10 mu M sulfamonomethoxine, and washing for 5 times by water.

3) And (3) fertility restoration identification: the sign of fertility restoration of the lily distant hybrid is that the pollen is fertile, the untreated pollen is sterile, and the pollen cannot germinate under a microscope. The fertile pollen grains obtained by the treatment have great power, and pollen can be seen to germinate and form pollen tubes under a microscope. Pollinating the pollen of the lily to the parent lily variety to produce a hybrid progeny; meanwhile, the female gamete can be bred, and can also seed after pollination to produce filial generation.

4) Breeding of filial generation: after the fertility of the distant hybridization first generation is restored, the main purpose is to generate a large amount of filial generation and cultivate a new excellent variety. After the distant hybrid and the parent variety are hybridized and fructified, the fruit is fully expanded in about 60 days, and a large number of hybrid plants can be obtained by adopting the conventional bead matching culture and embryo culture methods, so that a good new lily distant hybrid variety is bred.

Of course, the above is only a specific application example of the present invention, and other embodiments of the present invention are also within the scope of the present invention.

Claims (1)

1. A method for rapidly restoring fertility of lily distant hybrids is characterized in that: the method comprises the following steps:

1) cultivating distant lilies: selecting healthy distant lilies without diseases and insect pests, and carrying out lily cultivation management according to a conventional method, wherein the circumference of the bulb is larger than 12 cm;

2) and (3) lily bud treatment: selecting flowering distant lily bulbs, cultivating by a conventional method, dipping 4-10 mu M sulfamonomethoxine solution into cotton balls when flower buds appear, covering the flower buds on stem tips, treating flower buds of the distant lily bulbs for 2-3 days by 4-10 mu M sulfamonomethoxine, and washing for 5 times by water;

3) and (3) fertility restoration identification: observing the fertile pollen grains obtained after fertility restoration under a microscope, observing pollen germination and forming a pollen tube under the microscope, and pollinating the pollen on the parent lily variety to generate a filial generation; meanwhile, the female gamete can be fertile, and can also bear fruits after pollination to generate filial generation;

4) breeding of filial generation: after the distant hybrid and the parent variety are hybridized and fructified, the hybrid plant can be obtained by adopting the conventional bead matching culture and embryo culture method, and an excellent new lily distant hybrid variety is bred from the hybrid plant.