CN103766410B - A kind of plant resource nematocide and uses thereof preventing and treating Radix Angelicae Sinensis fiber crops stomatosis - Google Patents
A kind of plant resource nematocide and uses thereof preventing and treating Radix Angelicae Sinensis fiber crops stomatosis Download PDFInfo
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Abstract
The invention discloses a kind of plant resource nematocide and uses thereof preventing and treating Radix Angelicae Sinensis fiber crops stomatosis.Nematocide is made up of stellera chamaejasme L extract and diatomite proportioning, and it is 2-15% that each component and content mass percentage count stellera chamaejasme L extract content, and diatomite content is 85-98%.The present invention has significant inhibitory action to Radix Angelicae Sinensis fiber crops stomatosis, and can improve the output of Radix Angelicae Sinensis significantly, promotes the product phase of Radix Angelicae Sinensis, quality and medical value, and it is environmentally friendly, noresidue, can apply, have good economy and ecological benefits in Radix Angelicae Sinensis plant development.
Description
Technical field
The present invention relates to a kind of plant resource nematocide and uses thereof effectively preventing and treating Radix Angelicae Sinensis fiber crops stomatosis.Concretely, the present invention relates to a kind of is the plant resource nematocide of main component by Chinese Stellera Root, belongs to pesticide field.
Background technology
Radix Angelicae Sinensis is one of famous traditional Chinese medicine of China.In Radix Angelicae Sinensis planting process, harm seriously and the most common be exactly Radix Angelicae Sinensis fiber crops stomatosis, in order to return, root root epidermis be yellowish-brown lobe to main manifestations, hair root increases and abnormalization, and time serious, cortical tissue is in doing rotten, the rotten shape of chaff.Previously had research to confirm caused by Radix Angelicae Sinensis fiber crops stomatosis system eelworm harm, its pathogenic nematode be Ditylenchus destructor (
ditylenchusdestructor).But by the end of at present, do not have a kind of with strong points, action effect is obvious and environment amenable control Radix Angelicae Sinensis fiber crops stomatosis biogenic pesticide preparation, drastically influence the output of Radix Angelicae Sinensis medicinal material, quality and economic benefit, also constrain Radix Angelicae Sinensis plantation industry development.
Chinese Stellera Root (
stellerachamaejasmel.) be Thymelaeceae stellera herbaceos perennial, be distributed widely in meadow and the high mountain area without shade in the area such as China northeast, North China, southwest and northwest, plant resources enriches, and is one of poisonous plant main on northern China grassland.Result of study shows, stellera chamaejasme L extract has contact toxicity (ZL patent No. 1714641A) to insects such as cabbage caterpillar, cabbage caterpillar, aphids, also can be used as except mite agent, bacteria remover etc., but be used for but having no report to Radix Angelicae Sinensis fiber crops stomatosis study on prevention about stellera chamaejasme L extract.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and a kind of plant resource nematocide preventing and treating Radix Angelicae Sinensis fiber crops stomatosis is provided.
Another object of the present invention is to provide a kind of purposes of preventing and treating the plant resource nematocide of Radix Angelicae Sinensis fiber crops stomatosis.
A kind of plant resource nematocide preventing and treating Radix Angelicae Sinensis fiber crops stomatosis, it is characterized in that nematocide is made up of stellera chamaejasme L extract and diatomite proportioning, it is 2-15% that each component and content mass percentage count stellera chamaejasme L extract content, and diatomite content is 85-98%; Prepare by following method successively:
A, by Chinese Stellera Root through drying, pulverize, sieve, repeatedly extract by alcohol immersion; Extract, through filtration, reduced pressure concentration, drying, obtains stellera chamaejasme L extract;
B, with water or ethanol water by stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizing cracker to pulverize, crossing 45 mesh sieves, obtaining evengranular nematocide.
In step A, the mass ratio of Chinese Stellera Root and ethanol is 1:6-20.
In step A, the number of times repeatedly extracted is 2 to 5 times, and each extraction time is 20 to 30 hours.
The plant resource nematocide of control Radix Angelicae Sinensis fiber crops stomatosis of the present invention is graininess, can be directly used in agricultural production.
We have carried out the mensuration of relevant physical and chemical index to nematocide, and method is as follows:
Undertaken by 5.3.3 in GB GB/T1605-2001 " solid pharmaceutical preparation sampling " method.Determine the package of sampling by table of random number method, final amount of sampling is no less than 600g.
(1) bulk density and bulk density detect
The sample of known quality is put into glass cylinder, measures its volume, calculate bulk density.Then graduated cylinder is vertically improved 25mm, drop on rubber blanket, repeat the volume of working sample again after 100 times, calculate bulk density.
Record according to above method, root of Stellera chamaejasme extract quality percentage composition is the bulk density of 10% preparation (hereinafter referred to as 10% preparation) is 0.322g/mL, and bulk density is 0.428g/mL; Root of Stellera chamaejasme extract quality percentage composition is the bulk density of 5% preparation (hereinafter referred to as 5% preparation) is 0.300g/mL, and bulk density is 0.401g/mL; Root of Stellera chamaejasme extract quality percentage composition is the bulk density of 2% preparation (hereinafter referred to as 2% preparation) is 0.293g/mL, and bulk density is 0.389g/mL.
(2) pH measures
PH value is carried out according to GB GB/T1601.1g sample is dissolved in 100mL distilled water, stirs 1min, measure with the pH meter demarcated.10% preparation pH value is 6.04,5% preparation pH value be 6.36,2% preparation pH value is 6.48.
(3) heat storage stability test
By the heating storage that do not pressurize, make product accelerated ageing, the change of prediction storage at normal temperature properties of product.Get 20g sample and put into wide-mouth bottle, do not add any pressure, add a cover and put vial in baking oven, storage 14 days at (54 ± 2) DEG C.Take out vial, put into drier, be cooled to room temperature, in 24h, complete the detection to product active ingredient.
The change of high performance liquid chromatography to product active ingredient is utilized to detect.
Instrument and equipment: Agilent-1200 high performance liquid chromatograph; C18 reverse-phase chromatographic column (4.6mm × 250mm, 5 μm, C18Phecda filler); Methyl alcohol is chromatographically pure, and water is ultra-pure water, and it is pure that other reagent are analysis.
Chromatographic condition is 0-5min, 15% methyl alcohol; 5-10min, 15-30% methyl alcohol; 10-20min, 30-45% methyl alcohol; 20-40min, 45-65% methyl alcohol; 40-45min, 65-100% methyl alcohol.The method is linearly investigated, this detection method is linearly investigated (r>0.9993), precision (RSD=0.9%), repeatability (RSD=3.1%), stability (RSD=1.0%) and recovery test (rate of recovery >96.0%, RSD=1.3%).This detection method is stable, favorable reproducibility.
Test specimen processing method: get testing sample 1g, is dissolved in 30mL methyl alcohol, ultrasonic extraction 45min.Get 1.25mL in 1.5mL centrifuge tube, centrifugal 5min, getting supernatant miillpore filter (0.45 μm) and filter, is liquid to be measured.All test 10% preparation, 5% preparation and 2% preparation, each concentration extracts 3 samples.By by under 254nm, (retention time is respectively 5.7 to the larger peak area of 6 content under each concentration after overtesting process in sample, 15.5,38.5,43.5,46.8,49.7min) with this concentration under compare without testing processing sample, result shows that peak area rate of change is all within 10%.Absolutely prove that said preparation can be preserved for a long time, stable in properties, not easily occur to decompose change, be convenient to storage and transport.
The present invention, as control Radix Angelicae Sinensis fiber crops stomatosis, is characterized in that there is inhibitory action to Radix Angelicae Sinensis fiber crops stomatosis, and in Radix Angelicae Sinensis transplanting time and vegetative period process, be applied to Radix Angelicae Sinensis plantation around in 1-8cm place soil, every strain chemical applied to plants amount is at 2-6g.
The present invention has significant inhibitory action to Radix Angelicae Sinensis fiber crops stomatosis, and can improve the output of Radix Angelicae Sinensis significantly, promotes the product phase of Radix Angelicae Sinensis, quality and medical value, and it is environmentally friendly, noresidue, can apply, have good economy and ecological benefits in Radix Angelicae Sinensis plant development.
Effect that in preparation, component has is described below:
Diatomite used in preparation has that porosity is large, absorbability is strong, stable chemical nature, the feature such as wear-resisting, heat-resisting, and unit weight is light, and fineness is even, and pH value neutrality is nontoxic, and mixing uniformity is good.Utilize and diatomaceously enrich hole and strong absorption property is used as farm chemical carrier, medicament uniform adsorption can be reached and dilute the object of disperse, make that drug effect extends, toxicity alleviation, dosage easily controls.In addition diatomite can become concentrated fertilizer, impels the aspect effect such as crop growth, improvement soil.
In this plant resource nematocide, component used all derives from natural world, pure natural, easily degrades, and low toxicity is pollution-free, environmentally friendly, can meet the requirement of medicinal material plantation.
Plant resource nematocide provided by the invention, raw material is easy to get, with low cost; complete preparation when not adding the height poison of any chemosynthesis, high residue additive and auxiliary material, technique is simple, easily accomplishes scale production; this granular preparation is even, stable, can directly use.This product is efficient, low toxicity, noresidue.
In the preparation method of the plant resource nematocide provided in the present invention, utilize ethanol to prepare stellera chamaejasme L extract, because ethanol is easy to infiltrate plant tissue, and dissolubility is better, and extracts active ingredients is abundant; Utilize in preparation process ethanol water also have cheap, be easy to reclaim, to outstanding advantages such as human toxicity are little, be suitable for extensive preparation.
Except the control efficiency to Radix Angelicae Sinensis fiber crops stomatosis, Radix Angelicae Sinensis output and exterior quality have carried out statistical analysis, evaluated main pharmacodynamics composition in Radix Angelicae Sinensis by the method for high performance liquid chromatography, and measure the preparation residual condition of Radix Angelicae Sinensis sample, method is as follows:
1. the inherent effective component evaluation of Radix Angelicae Sinensis
High performance liquid chromatography is utilized to establish Radix Angelicae Sinensis active ingredient finger-print, the content of forulic acid and Ligustilide in each group of Radix Angelicae Sinensis of field trial is detected, find that said preparation effectively can improve the content of ferulic acid from Chinese angelica and Ligustilide, improve the medical value of Radix Angelicae Sinensis.
According to " Chinese Pharmacopoeia 2010 " regulation, forulic acid is the important indicator investigating Radix Angelicae Sinensis interior quality.And the content of Ligustilide from Angelica sinensis, also often by the important parameter as evaluation Radix Angelicae Sinensis quality.By adopting the method for finger-print, with the content of forulic acid and Ligustilide for parameter, integrated quality evaluation is carried out to the Radix Angelicae Sinensis experimental group of dispenser Chinese Stellera Root.
Analytical method: from every experimental group, gets the Radix Angelicae Sinensis sample be of moderate size and dries in the shade, and pulverizes, by 45 object sieves.Get 9 grams of sample powder, add 50% (v/v) methanol/water solution 180mL, ultrasonic extraction 45min.By centrifugal for extract 15min, rotating speed 6000rpm, gets supernatant, then with after 0.45 μm of membrane filtration, for subsequent use.
Agilent1200 series of high efficiency liquid chromatograph; C18 reverse-phase chromatographic column (4.6mm × 250mm, 5 μm, C18Sinochrom filler); Mobile phase A: 0.5% (v/v) acetic acid aqueous solution, Mobile phase B: methyl alcohol; Gradient condition: 0minB30%, 20minB70%, 70minB100%; UV detect wavelength is 275nm, and reference wavelength is 400nm; Sampling volume 20 μ L; Flow velocity 0.6mL/min; Column temperature 30 DEG C; Biphenyl is internal standard compound.And the method is linearly investigated (r>0.9995), precision (RSD=2.1%), repeatability (RSD=2.3%), stability (RSD=0.6%) and recovery test (rate of recovery >98.5%, RSD=0.9%).The method is stable, precision is high, favorable reproducibility.Each group repeats extraction 3 samples.
2. residue detection in Radix Angelicae Sinensis sample
The selection of 2.1 standard items
In order to improve detection sensitivity, the peak (Stellera chamaejasme chromone, 7-methoxyl group chamaejasmine, chamaejasmine C retention time are respectively the peak of 38.0,42.9 and 46.5min) choosing topmost 3 compositions in preparation is standard items.Utilize standard items monomer to draw concentration curve, determine that lowest detection line is 1.0 × 10
-6.
2.2 conditional filterings and optimization
Get Radix Angelicae Sinensis to be measured to dry in the shade, pulverize, by 45 object sieves, for subsequent use.Be optimized by the Extraction solvent to Radix Angelicae Sinensis sample, extracted amount liquor ratio, extraction time and chromatographic condition, and the method is linearly investigated (r>0.9996), precision (RSD=1.1%), repeatability (RSD=2.1%), stability (RSD=0.4%) and recovery test (rate of recovery >98.0%, RSD=1.0%).Verified, this detection method is stable, favorable reproducibility, may be used for the residue detection of preparation.Sample treatment is: get and be about 0.1g for examination Radix Angelicae Sinensis sample, accurately weighed, is placed in 15mL tool plug test tube, precision adds 10mL methyl alcohol, after ultrasonic 45min, gets 1.25mL in 1.5mL centrifuge tube, centrifugal 5min, getting supernatant miillpore filter (0.45 μm) and filter, is liquid to be measured.
Testing conditions is: 0-10min, 43% methyl alcohol; 10-20min, 43%-58% methyl alcohol.
Field trial sample tests shows, the nematocide that the present invention develops can significantly improve the content of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis, and noresidue in Radix Angelicae Sinensis sample, safely, effectively, reliably.
The present invention have developed a kind of novel effectively, the plant resource nematocide of environmental protection, effectively reduce the incidence of disease and the extent of injury of Radix Angelicae Sinensis fiber crops stomatosis, improve the yield and quality of Radix Angelicae Sinensis, Chinese Stellera Root is changed harmful to treasure simultaneously, for China's western grass poisonous weeds damage control and Grassland recover to open up a positive development and utilization measure, to China's agricultural nematodosis control and animal husbandry development significant.
Embodiment
Embodiment 1
Prepare 2% preparation, method is as follows:
By root of Stellera chamaejasme through drying, pulverizing, sieve, repeatedly extract 3 times by the alcohol immersion of 10 times of quality, each 24 hours; Extract, through filtration, reduced pressure concentration, drying, obtains stellera chamaejasme L extract;
With water or ethanol water by stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizing cracker to pulverize, crossing 45 mesh sieves, obtaining evengranular nematocide.Wherein root of Stellera chamaejasme extract and diatomite proportioning composition in preparation, count root of Stellera chamaejasme extractive content for 2% with mass percentage, diatomite content is 98%.
Radix Angelicae Sinensis plantation field trial has been carried out on Stochastic choice continuous print 6 ridge, Qin Xu township, Minxian County, Dingxi City, Radix Angelicae Sinensis main growing area Gansu Province.Every ridge all long 8m in ground, respectively is control group (CK), 2% preparation dispenser group.At Radix Angelicae Sinensis transplanting time, 3cm place around root preparation 3g being evenly sprinkling upon Radix Angelicae Sinensis seedling.
Ocular estimate standard:
Fiber crops stomatosis classification: disease area is less than 1/3 for one-level; It is secondary between 1/3 and 2/3; Being greater than 2/3 is three grades.
Quality standard: being greater than 100g is one-level; It is secondary between 50g to 100g; Below 50g is three grades.
Leaf spot classification: disease area is less than 1/3 for one-level; It is secondary between 1/3 and 2/3; Being greater than 2/3 is three grades.
Output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation the results are shown in following table:
| Output (Kg) | Fiber crops stomatosis is without (%) | Fiber crops stomatosis one-level (%) | Fiber crops stomatosis secondary (%) | Fiber crops stomatosis three grades (%) | Quality one-level (%) | Quality secondary (%) | Quality three grades (%) | Reed head (cm) | Root long (cm) | Side radical (cm) | |
| CK | 7.42 | 53.98 | 19.46 | 17.69 | 9.73 | 28.84 | 39.22 | 31.94 | 1.70 | 15.18 | 2.27 |
| 2% | 8.75 | 57.81 | 25.00 | 10.93 | 6.25 | 37.03 | 34.17 | 28.80 | 2.25 | 20.67 | 1.80 |
Table 1.2% preparation group output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation result
The numb stomatosis incidence of disease of control group is 46.88%, and dispenser sequela rate obviously reduces, and the 2% preparation group incidence of disease is reduced to 42.18%; And incidence is also very significantly improved after administration, endangers serious secondary, three grades of numb stomatosis incidences of disease significantly reduce, the sick incidence of disease of control group three grades is 9.73%, and the sick incidence of disease of 2% preparation group three grades is only 6.25%.
The output of Radix Angelicae Sinensis and ocular estimate are directly connected to the economic benefit of Radix Angelicae Sinensis.The wherein reed head size of Radix Angelicae Sinensis, root length and side radical are the important indicators of Radix Angelicae Sinensis ocular estimate, and the Radix Angelicae Sinensis that good quality, price are higher, its reed head diameter is large, and main root is longer, and side root is less.
Statistical result showed, the gross yield of dispenser group Radix Angelicae Sinensis will apparently higher than control group, and the gross yield of control group is 7.42kg, and 2% preparation group is 8.75kg.Control group reed head average diameter is 1.70cm, and 2% preparation group average diameter is 2.25cm; Control group average root is long is 15.18cm, and 2% preparation group average root length is 20.67cm; Control group average side root 2.27, and the average side radical of 2% preparation group is 1.80.
While the numb stomatosis of control, said preparation can also effectively improve Radix Angelicae Sinensis emergence rate, reduces the bolting of Radix Angelicae Sinensis, thus improves the patent medicine rate of Radix Angelicae Sinensis, and then make Radix Angelicae Sinensis realize increasing both production and income (see table 2).
| Emerge and count (strain) | Early a kind of sedge number (strain) | Early a kind of sedge rate (%) | Become a useful person and count (strain) | Lumber recovery (%) | |
| CK | 317 | 52 | 16.40 | 114 | 35.96 |
| 2% | 364 | 70 | 19.23 | 128 | 35.16 |
The impact that table 2.2% preparation group is emerged on Radix Angelicae Sinensis and become a useful person
In field test results display, said preparation has certain preventive and therapeutic effect (see table 3) equally to Radix Angelicae Sinensis leaf spot.
| Leaf spot | Without (%) | One-level (%) | Secondary (%) | Three grades (%) |
| CK | 10.06 | 50.65 | 33.33 | 6.67 |
| 2% | 16.67 | 63.33 | 16.67 | 3.33 |
Table 3.2% preparation group is on the impact of Radix Angelicae Sinensis leaf spot
The Radix Angelicae Sinensis fingerprint spectrum method utilizing the present invention to set up, to the content detection result of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis as following table:
| Forulic acid (peak area) | Ligustilide (peak area) | |
| CK | 1521.958 | 25232.77 |
| 2% | 1573.826 | 26618.29 |
Table 4.2% preparation group is on the impact of effective component in Radix Angelicae Sinensis
The sample of method for detecting residue to 2% preparation group set up in the present invention is utilized to detect.In 3 samples extracted, all do not detect that preparation remains.
Embodiment 2
Prepare 5% preparation, method is as follows:
By root of Stellera chamaejasme through drying, pulverizing, sieve, repeatedly extract 4 times by the alcohol immersion of 12 times of quality, each 26 hours; Extract, through filtration, reduced pressure concentration, drying, obtains stellera chamaejasme L extract;
With water or ethanol water by stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizing cracker to pulverize, crossing 45 mesh sieves, obtaining evengranular nematocide.Wherein root of Stellera chamaejasme extract and diatomite proportioning composition in preparation, count root of Stellera chamaejasme extractive content for 5% with mass percentage, diatomite content is 95%.
Radix Angelicae Sinensis plantation field trial has been carried out on Stochastic choice continuous print 6 ridge, Qin Xu township, Minxian County, Dingxi City, Radix Angelicae Sinensis main growing area Gansu Province.Every ridge all long 8m in ground, respectively is control group (CK), 5% preparation dispenser group.4cm place around root preparation 3g being evenly sprinkling upon Radix Angelicae Sinensis seedling.
Output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation the results are shown in following table:
| Output (Kg) | Fiber crops stomatosis is without (%) | Fiber crops stomatosis one-level (%) | Fiber crops stomatosis secondary (%) | Fiber crops stomatosis three grades (%) | Quality one-level (%) | Quality secondary (%) | Quality three grades (%) | Reed head (cm) | Root long (cm) | Side radical (cm) | |
| CK | 7.42 | 53.98 | 19.46 | 17.69 | 9.73 | 28.84 | 39.22 | 31.94 | 1.70 | 15.18 | 2.27 |
| 5% | 8.52 | 76.92 | 7.69 | 8.47 | 6.92 | 29.81 | 37.21 | 32.98 | 2.02 | 21.97 | 1.97 |
Table 5.5% preparation group output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation result
The numb stomatosis incidence of disease of control group is 46.88%, and dispenser sequela rate obviously reduces, and the 5% preparation group incidence of disease is significantly reduced to 23.08%; And incidence is also very significantly improved after administration, endangers serious secondary, three grades of numb stomatosis incidences of disease reduce, the sick incidence of disease of control group three grades is 9.73%, and the sick incidence of disease of 5% preparation group three grades is only 6.92%.
The gross yield of dispenser group Radix Angelicae Sinensis will apparently higher than control group, and the gross yield of control group is 7.42kg, and 5% preparation group is 8.52kg.Control group reed head average diameter is 1.70cm, and 5% preparation group average diameter is 2.02cm; Control group average root is long is 15.18cm, and 5% preparation group average root length is 21.97cm; Control group average side root 2.27, and 5% preparation group average side radical is 1.97.
While the numb stomatosis of control, said preparation can also effectively improve Radix Angelicae Sinensis emergence rate, reduces the bolting of Radix Angelicae Sinensis, thus improves the patent medicine rate of Radix Angelicae Sinensis, and then make Radix Angelicae Sinensis realize increasing both production and income (see table 6).
| Emerge and count (strain) | Early a kind of sedge number (strain) | Early a kind of sedge rate (%) | Become a useful person and count (strain) | Lumber recovery (%) | |
| CK | 317 | 52 | 16.40 | 114 | 35.96 |
| 5% | 357 | 53 | 14.85 | 130 | 36.41 |
The impact that table 6.5% preparation group is emerged on Radix Angelicae Sinensis and become a useful person
In field test results display, 5% preparation group has certain preventive and therapeutic effect (see table 7) equally to Radix Angelicae Sinensis leaf spot.
| Leaf spot | Without (%) | One-level (%) | Secondary (%) | Three grades (%) |
| CK | 10.06 | 50.65 | 33.33 | 6.67 |
| 5% | 20.07 | 56.67 | 20.08 | 3.18 |
Table 7.5% preparation group is on the impact of Radix Angelicae Sinensis leaf spot
The Radix Angelicae Sinensis fingerprint spectrum method utilizing the present invention to set up, to the content detection result of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis as following table:
| Forulic acid (peak area) | Ligustilide (peak area) | |
| CK | 1521.958 | 25232.77 |
| 5% | 1871.017 | 26847.44 |
Table 8.5% preparation group is on the impact of effective component in Radix Angelicae Sinensis
5% preparation group can significantly improve the content of drug effect components of Radix Angelicae Sinensis, improves the medical value of Radix Angelicae Sinensis.Wherein the content of forulic acid exceeds 22.93% than contrast; The content of Ligustilide exceeds 6.40% than contrast.
The sample of method for detecting residue to 5% preparation group set up in the present invention is utilized to detect.In 3 samples extracted, all do not detect that preparation remains.
Embodiment 3
Prepare 10% preparation, method is as follows:
By root of Stellera chamaejasme through drying, pulverizing, sieve, repeatedly extract 5 times by the alcohol immersion of 11 times of quality, each 20 hours; Extract, through filtration, reduced pressure concentration, drying, obtains stellera chamaejasme L extract;
With water or ethanol water by stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizing cracker to pulverize, crossing 45 mesh sieves, obtaining evengranular nematocide.Wherein root of Stellera chamaejasme extract and diatomite proportioning composition in preparation, count root of Stellera chamaejasme extractive content for 10% with mass percentage, diatomite content is 90%.
Radix Angelicae Sinensis plantation field trial has been carried out on Stochastic choice continuous print 6 ridge, Qin Xu township, Minxian County, Dingxi City, Radix Angelicae Sinensis main growing area Gansu Province.Every ridge all long 8m in ground, respectively is control group (CK), 10% preparation dispenser group.3cm place around root preparation 4g being evenly sprinkling upon Radix Angelicae Sinensis seedling.
Output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation the results are shown in following table:
| Output (Kg) | Fiber crops stomatosis is without (%) | Fiber crops stomatosis one-level (%) | Fiber crops stomatosis secondary (%) | Fiber crops stomatosis three grades (%) | Quality one-level (%) | Quality secondary (%) | Quality three grades (%) | Reed head (cm) | Root long (cm) | Side radical (cm) | |
| CK | 7.42 | 53.98 | 19.46 | 17.69 | 9.73 | 28.84 | 39.22 | 31.94 | 1.70 | 15.18 | 2.27 |
| 10% | 9.42 | 74.03 | 12.99 | 11.04 | 1.94 | 24.42 | 39.17 | 36.41 | 2.39 | 20.80 | 1.73 |
Table 9.10% preparation group output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation result
The numb stomatosis incidence of disease of control group is 46.88%, and dispenser sequela rate obviously reduces, and the 10% preparation group incidence of disease is significantly reduced to 25.97%; And incidence is also very significantly improved after administration, endangers serious secondary, three grades of numb stomatosis incidences of disease significantly reduce, the sick incidence of disease of control group three grades is 9.73%, and the sick incidence of disease of 10% preparation group three grades is only 1.94%.
The gross yield of dispenser group Radix Angelicae Sinensis will apparently higher than control group, and the gross yield of control group is 7.42kg, and 10% preparation group is 9.42kg.Control group reed head average diameter is 1.70cm, and 10% preparation group average diameter is 2.39cm; Control group average root is long is 15.18cm, and 10% preparation group average root length is 20.80cm; Control group average side root 2.27, and 10% preparation group average side radical is 1.73.
While the numb stomatosis of control, said preparation can also effectively improve Radix Angelicae Sinensis emergence rate, reduces the bolting of Radix Angelicae Sinensis, thus improves the patent medicine rate of Radix Angelicae Sinensis, and then make Radix Angelicae Sinensis realize increasing both production and income (see table 10).
| Emerge and count (strain) | Early a kind of sedge number (strain) | Early a kind of sedge rate (%) | Become a useful person and count (strain) | Lumber recovery (%) | |
| CK | 317 | 52 | 16.40 | 114 | 35.96 |
| 10% | 367 | 72 | 19.62 | 154 | 41.96 |
The impact that table 10.10% preparation group is emerged on Radix Angelicae Sinensis and become a useful person
In field test results display, said preparation has certain preventive and therapeutic effect (see table 7) equally to Radix Angelicae Sinensis leaf spot.
| Leaf spot | Without (%) | One-level (%) | Secondary (%) | Three grades (%) |
| CK | 10.06 | 50.65 | 33.33 | 6.67 |
| 10% | 26.67 | 53.33 | 20.00 | 0 |
Table 11.10% preparation group is on the impact of Radix Angelicae Sinensis leaf spot
Utilize the Radix Angelicae Sinensis fingerprint spectrum method set up in the present invention, to the content detection result of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis as following table:
| Forulic acid (peak area) | Ligustilide (peak area) | |
| CK | 1521.958 | 25232.77 |
| 10% | 2240.5 | 29208.45 |
Table 12.10% preparation group is on the impact of effective component in Radix Angelicae Sinensis
10% preparation group significantly improves the content of drug effect components of Radix Angelicae Sinensis, improves the medical value of Radix Angelicae Sinensis.Wherein the content of forulic acid exceeds 47.21% than contrast; The content of Ligustilide exceeds 15.76% than contrast.
The sample of method for detecting residue to 10% preparation group set up in the present invention is utilized to detect.In 3 samples extracted, all do not detect that preparation remains.
Claims (2)
1. a plant resource nematocide, it is characterized in that nematocide is made up of the Chinese Stellera Root root extract being main component with Stellera chamaejasme chromone, 7-methoxyl group chamaejasmine and chamaejasmine C and diatomite proportioning, it is 2-15% that each component and content mass percentage count root of Stellera chamaejasme extractive content, and diatomite content is 85-98%; Prepare by following method successively:
A, by root of Stellera chamaejasme through drying, pulverize, sieve, repeatedly extract by alcohol immersion; Extract, through filtration, reduced pressure concentration, drying, obtains Chinese Stellera Root root extract; Wherein the mass ratio of Chinese Stellera Root and ethanol is 1:6-20, and the number of times repeatedly extracted is 2 to 5 times, and each extraction time is 20 to 30 hours;
B, with water or ethanol water by Chinese Stellera Root root extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizing cracker to pulverize, crossing 45 mesh sieves, obtaining evengranular nematocide.
2. the purposes of a nematocide control Radix Angelicae Sinensis fiber crops stomatosis as claimed in claim 1, it is characterized in that there is inhibitory action to Radix Angelicae Sinensis fiber crops stomatosis, in Radix Angelicae Sinensis transplanting time and vegetative period process, be applied to Radix Angelicae Sinensis plantation around in 1-8cm place soil, every strain chemical applied to plants amount is at 2-6g.
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| CN105669315A (en) * | 2016-02-22 | 2016-06-15 | 甘肃省农业科学院植物保护研究所 | Plant source radix euphorbiae lantu insecticide and fertilizer mixture granule and preparation method |
| CN106105613A (en) * | 2016-06-24 | 2016-11-16 | 渭源县鹿鸣现代农业专业合作社 | A kind of prevention and controls of Radix Angelicae Sinensis fiber crops stomatosis |
| CN107114414A (en) * | 2017-05-25 | 2017-09-01 | 中国科学院西北高原生物研究所 | The application of the anti-Wolfberry Aphid of The Leaves And Stems of Stellera Chamaejasme extract |
| CN108033858B (en) * | 2017-12-19 | 2020-09-22 | 河南省农业科学院园艺研究所 | Special clay soil conditioner for facility agriculture |
| CN112903876B (en) * | 2021-03-02 | 2022-04-01 | 中国科学院兰州化学物理研究所 | Method for extracting root soil and root allelochemical substances of stellera chamaejasme and detecting content of root allelochemical substances |
| CN116210709B (en) * | 2023-03-14 | 2024-06-28 | 郑州大学 | Application of 5-O-methyl-vidamiloride in plant root system development |
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