CN103766410A - Plant-source nematicide for preventing and curing ditylenchus destructor of angelica sinensis, and application of nematicide - Google Patents
Plant-source nematicide for preventing and curing ditylenchus destructor of angelica sinensis, and application of nematicide Download PDFInfo
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Abstract
The invention discloses a plant-source nematicide for preventing and curing ditylenchus destructor of angelica sinensis, and application of the plant-source nematicide. The nematicide is prepared form 2-15% of stellera chamaejasme extrct and 85-98% of kieselguhr in percentage by weight. The plant-source nematicide has a remarkable inhibiting effect on the ditylenchus destructor of angelica sinensis, in addition, the yield of the angelica sinensis is remarkably increased, the condition, the quality and the medicinal value of the angelica sinensis are improved, the nematicide is environmentally-friendly, does not have any residue, is popularized and applied in the plant production mode of the angelica sinensis, and has excellent economic and ecological benefits.
Description
Technical field
The present invention relates to a kind of plant resource nematocide that can effectively prevent and treat Radix Angelicae Sinensis fiber crops stomatosis and uses thereof.Concretely, the present invention relates to a kind of plant resource nematocide that is main component by Chinese Stellera Root, belong to pesticide field.
Background technology
Radix Angelicae Sinensis is one of famous traditional Chinese medicine of China.In Radix Angelicae Sinensis planting process, endangering serious and the most common is exactly Radix Angelicae Sinensis fiber crops stomatosis, main manifestations for returning root root epidermis to be yellowish-brown lobe, hair root increases and abnormalization, when serious, cortical tissue is and does rotten, the rotten shape of chaff.Previously studies confirm that Radix Angelicae Sinensis fiber crops stomatosis was due to eelworm harm, its pathogenic nematode be Ditylenchus destructor (
ditylenchus destructor).But by the end of at present, do not have a kind of with strong points, action effect is obvious and the biogenic pesticide preparation of environment amenable control Radix Angelicae Sinensis fiber crops stomatosis, is having a strong impact on output, quality and the economic benefit of Radix Angelicae Sinensis medicinal material, has also restricted Radix Angelicae Sinensis plantation industry development.
Chinese Stellera Root (
stellera chamaejasmel.) be Thymelaeceae stellera herbaceos perennial, be distributed widely in meadow and the high mountain area without shade in the areas such as China northeast, North China, southwest and northwest, plant resources is abundant, is one of poisonous plant main on northern China grassland.Result of study shows, stellera chamaejasme L extract has contact toxicity (ZL patent No. 1714641A) to insects such as cabbage caterpillar, cabbage caterpillar, aphids, also can be used as except mite agent, bacteria remover etc., but be used for Radix Angelicae Sinensis fiber crops stomatosis study on prevention but to have no report about stellera chamaejasme L extract.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and a kind of plant resource nematocide of preventing and treating Radix Angelicae Sinensis fiber crops stomatosis is provided.
Another object of the present invention is to provide a kind of purposes of the plant resource nematocide of preventing and treating Radix Angelicae Sinensis fiber crops stomatosis.
A kind of plant resource nematocide of preventing and treating Radix Angelicae Sinensis fiber crops stomatosis, it is characterized in that nematocide is made up of stellera chamaejasme L extract and diatomite proportioning, it is 2-15% that each component and content are counted stellera chamaejasme L extract content with quality percentage composition, and diatomite content is 85-98%; Prepare by following method successively:
A, by Chinese Stellera Root through drying, pulverize, sieve, repeatedly extract by alcohol immersion; Extract, through filtration, reduced pressure concentration, dry, makes stellera chamaejasme L extract;
B, water or ethanol water be stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizes cracker to pulverize, and crosses 45 mesh sieves, makes evengranular nematocide.
In A step, the mass ratio of Chinese Stellera Root and ethanol is 1:6-20.
In A step, the number of times repeatedly extracting is 2 to 5 times, and each extraction time is 20 to 30 hours.
The plant resource nematocide of control Radix Angelicae Sinensis fiber crops stomatosis of the present invention is graininess, can be directly used in agricultural production.
We have carried out the mensuration of relevant physical and chemical index to nematocide, method is as follows:
Undertaken by 5.3.3 in GB GB/T 1605-2001 " solid pharmaceutical preparation sampling " method.Determine the package of sampling by table of random number method, final amount of sampling is no less than 600 g.
(1) bulk density and bulk density detect
The sample of known quality is put into glass cylinder, measure its volume, calculate bulk density.Then by vertical graduated cylinder 25 mm that improve, drop on rubber blanket, repeats 100 times after the volume of working sample again, calculating bulk density.
Record according to above method, root of Stellera chamaejasme extract quality percentage composition is that the bulk density of 10% preparation (hereinafter to be referred as 10% preparation) is 0.322 g/mL, and bulk density is 0.428 g/mL; Root of Stellera chamaejasme extract quality percentage composition is that the bulk density of 5% preparation (hereinafter to be referred as 5% preparation) is 0.300 g/mL, and bulk density is 0.401 g/mL; Root of Stellera chamaejasme extract quality percentage composition is that the bulk density of 2% preparation (hereinafter to be referred as 2% preparation) is 0.293 g/mL, and bulk density is 0.389 g/mL.
(2) pH measures
PH value is carried out according to GB GB/T 1601.1 g sample is dissolved in 100 mL distilled water, stirs 1 min, by the pH meter mensuration of having demarcated.10% preparation pH value is that 6.04,5% preparation pH values are that 6.36,2% preparation pH values are 6.48.
(3) heat storage stability test
By the heating storage that do not pressurize, make product accelerated ageing, the variation of prediction storage at normal temperature properties of product.Get 20g sample and put into wide-mouth bottle, do not add any pressure, add a cover and put vial in baking oven, at (54 ± 2) ℃, store 14 days.Take out vial, put into drier, be cooled to room temperature, in 24 h, complete the detection to product active ingredient.
Utilize high performance liquid chromatography to detect the variation of product active ingredient.
Instrument and equipment: Agilent-1200 high performance liquid chromatograph; C18 reverse-phase chromatographic column (4.6mm × 250mm, 5 μ m, C18 Phecda filler); Methyl alcohol is chromatographically pure, and water is ultra-pure water, and it is pure that other reagent are analysis.
Chromatographic condition is 0-5 min, 15% methyl alcohol; 5-10 min, 15-30% methyl alcohol; 10-20 min, 30-45% methyl alcohol; 20-40 min, 45-65% methyl alcohol; 40-45 min, 65-100% methyl alcohol.The method is carried out linearity and is investigated, this detection method is carried out to linearity and investigate (r>0.9993), precision (RSD=0.9%), repeatability (RSD=3.1%), stability (RSD=1.0%) and recovery test (rate of recovery >96.0%, RSD=1.3%).This detection method is stable, favorable reproducibility.
Test specimen processing method: get testing sample 1 g, be dissolved in 30 mL methyl alcohol, ultrasonic extraction 45 min.Get 1.25 mL in 1.5 mL centrifuge tubes, centrifugal 5 min, (0.45 μ m) filters, and is liquid to be measured to get supernatant miillpore filter.10% preparation, 5% preparation and 2% preparation are all tested, and each concentration extracts 3 samples.By by under 254 nm, under each concentration after overtesting is processed 6 larger peak areas of content in sample (retention time is respectively 5.7,15.5,38.5,43.5,46.8,49.7 min) with this concentration under without test processing sample compare, result shows that peak area rate of change is all in 10%.Absolutely prove that said preparation can preserve for a long time, stable in properties, is difficult for occurring to decompose changing, and is convenient to store and transportation.
The present invention, as control Radix Angelicae Sinensis fiber crops stomatosis, is characterized in that Radix Angelicae Sinensis fiber crops stomatosis is had to inhibitory action, in Radix Angelicae Sinensis transplanting time and vegetative period process, is applied to Radix Angelicae Sinensis plantation around in 1-8 cm place soil, and every strain chemical applied to plants amount is at 2-6 g.
The present invention has significant inhibitory action to Radix Angelicae Sinensis fiber crops stomatosis, and can improve significantly the output of Radix Angelicae Sinensis, promotes product phase, quality and the medical value of Radix Angelicae Sinensis, and environmentally friendly, noresidue can be applied in Radix Angelicae Sinensis plant development, has good economy and ecological benefits.
Effect that in preparation, component has is described below:
In preparation, diatomite used has that porosity is large, absorbability is strong, stable chemical nature, the feature such as wear-resisting, heat-resisting, and unit weight is light, and fineness is even, and pH value neutrality is nontoxic, and mixing uniformity is good.Utilize the diatomaceous hole that enriches to be used as farm chemical carrier with strong absorption property, can reach the even object of disperseing with dilution of adsorbing of medicament, drug effect prolongation, toxicity alleviation, dosage are easily controlled.In addition diatomite can become concentrated fertilizer, impels the aspect effect such as crop growth, improvement soil.
In this plant resource nematocide, component used all derives from natural world, pure natural, and easily degraded, low toxicity, pollution-free, environmentally friendly, can meet the requirement of medicinal material plantation.
Plant resource nematocide provided by the invention, raw material is easy to get, with low cost; complete preparation in the case of not adding the height poison of any chemosynthesis, high residue additive and auxiliary material, technique is simple, easily accomplishes scale production; this granular preparation is even, stable, can directly use.This product is efficient, low toxicity, noresidue.
In the preparation method of the plant resource nematocide providing in the present invention, utilize ethanol to prepare stellera chamaejasme L extract, because ethanol is easy to infiltrate plant tissue, and dissolubility is better, and extracts active ingredients is abundant; In preparation process, utilize ethanol water also have cheap, be easy to reclaim, to outstanding advantages such as human toxicity are little, be suitable for extensive preparation.
Except the control efficiency to Radix Angelicae Sinensis fiber crops stomatosis, Radix Angelicae Sinensis output and exterior quality have been carried out statistical analysis, method by high performance liquid chromatography is evaluated main pharmacodynamics composition in Radix Angelicae Sinensis, and the preparation residual condition of Radix Angelicae Sinensis sample is measured, and method is as follows:
1. the inherent effective component evaluation of Radix Angelicae Sinensis
Utilize high performance liquid chromatography to set up Radix Angelicae Sinensis active ingredient finger-print, content to forulic acid and Ligustilide in each group of Radix Angelicae Sinensis of field trial detects, find that said preparation can effectively improve the content of ferulic acid from Chinese angelica and Ligustilide, has improved the medical value of Radix Angelicae Sinensis.
According to " Chinese pharmacopoeia 2010 " regulation, forulic acid is the important indicator of investigating Radix Angelicae Sinensis interior quality.And the content of Ligustilide from Angelica sinensis is also often used as the important parameter of evaluating Radix Angelicae Sinensis quality.By adopting the method for finger-print, take the content of forulic acid and Ligustilide as parameter, the Radix Angelicae Sinensis experimental group of dispenser Chinese Stellera Root is carried out to integrated quality evaluation.
Analytical method: from every experimental group, get the Radix Angelicae Sinensis sample being of moderate size and dry in the shade, pulverize, by 45 object sieves.Get 9 grams of sample powder, add 50% (v/v) methanol/water solution, 180 mL, ultrasonic extraction 45 min.By centrifugal extract 15 min, rotating speed 6000 rpm, get supernatant, then with after 0.45 μ m membrane filtration, for subsequent use.
Agilent 1200 series of high efficiency liquid chromatographs; C18 reverse-phase chromatographic column (4.6mm × 250mm, 5 μ m, C18 Sinochrom filler); Mobile phase A: 0.5% (v/v) acetic acid aqueous solution, Mobile phase B: methyl alcohol; Gradient condition: 0 min B 30%, 20 min B 70%, 70 min B 100%; It is 275 nm that ultraviolet detects wavelength, and reference wavelength is 400 nm; Sampling volume 20 μ L; Flow velocity 0.6 mL/min; 30 ℃ of column temperatures; Biphenyl is internal standard compound.And the method is carried out to linearity and investigate (r>0.9995), precision (RSD=2.1%), repeatability (RSD=2.3%), stability (RSD=0.6%) and recovery test (rate of recovery >98.5%, RSD=0.9%).The method is stable, precision is high, favorable reproducibility.Each group repeats to extract 3 samples.
2. residue detection in Radix Angelicae Sinensis sample
The selection of 2.1 standard items
In order to improve detection sensitivity, the peak (root of langdu chromone, 7-methoxyl group chamaejasmine, chamaejasmine C retention time are respectively the peak of 38.0,42.9 and 46.5 min) of choosing topmost 3 compositions in preparation is standard items.Utilize standard items monomer to draw concentration curve, determine that lowest detection line is 1.0 × 10
-6.
2.2 conditional filterings and optimization
Get Radix Angelicae Sinensis to be measured and dry in the shade, pulverize, by 45 object sieves, for subsequent use.Be optimized by the extraction solvent to Radix Angelicae Sinensis sample, extracted amount liquor ratio, extraction time and chromatographic condition, and the method is carried out to linearity and investigate (r>0.9996), precision (RSD=1.1%), repeatability (RSD=2.1%), stability (RSD=0.4%) and recovery test (rate of recovery >98.0%, RSD=1.0%).Verified, this detection method is stable, favorable reproducibility, can be for the residue detection of preparation.Sample treatment is: gets for examination Radix Angelicae Sinensis sample approximately 0.1 g, and accurately weighed, be placed in 15 mL tool plug test tubes, precision adds 10 mL methyl alcohol, after ultrasonic 45 min, gets 1.25 mL in 1.5 mL centrifuge tubes, centrifugal 5 min, (0.45 μ m) filters, and is liquid to be measured to get supernatant miillpore filter.
Testing conditions is: 0-10min, 43% methyl alcohol; 10-20min, 43%-58% methyl alcohol.
Field trial sample test result shows, the nematocide that the present invention develops can obviously improve the content of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis, and noresidue in Radix Angelicae Sinensis sample, safety, effectively, reliable.
The present invention developed a kind of novel effectively, the plant resource nematocide of environmental protection, effectively reduce the incidence of disease and the extent of injury of Radix Angelicae Sinensis fiber crops stomatosis, improve the yield and quality of Radix Angelicae Sinensis, Chinese Stellera Root is changed harmful to treasure simultaneously, for China's western grass poisonous weeds damage control and Grassland recover to open up a positive development and utilization measure, significant to China's agricultural nematodosis control and animal husbandry development.
Embodiment
Embodiment 1
Prepare 2% preparation, method is as follows:
Root of Stellera chamaejasme through drying, pulverize, sieve, is extracted 3 times to each 24 hours repeatedly by the alcohol immersion of 10 times of quality; Extract, through filtration, reduced pressure concentration, dry, makes stellera chamaejasme L extract;
Water or ethanol water be stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizes cracker to pulverize, and crosses 45 mesh sieves, makes evengranular nematocide.Wherein root of Stellera chamaejasme extract and diatomite proportioning composition in preparation, count root of Stellera chamaejasme extractive content as 2% take quality percentage composition, and diatomite content is 98%.
Select at random 6 continuous ridges in Qin Xu township, Minxian County, Dingxi City of Radix Angelicae Sinensis main growing area Gansu Province and carried out Radix Angelicae Sinensis plantation field trial.Every ridge all long 8m of ground, respectively are control group (CK), 2% preparation dispenser group.At Radix Angelicae Sinensis transplanting time, preparation 3 g are evenly sprinkling upon to the root 3 cm places around of Radix Angelicae Sinensis seedling.
Ocular estimate standard:
Fiber crops stomatosis classification: disease area is less than 1/3 for one-level; It between 1/3 and 2/3, is secondary; Being greater than 2/3 is three grades.
Quality standard: being greater than 100g is one-level; 50g is to being secondary between 100g; 50g is three grades below.
Leaf spot classification: disease area is less than 1/3 for one-level; It between 1/3 and 2/3, is secondary; Being greater than 2/3 is three grades.
Output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation the results are shown in following table:
| ? | Output (Kg) | Fiber crops stomatosis is without (%) | Fiber crops stomatosis one-levels (%) | Fiber crops stomatosis secondarys (%) | Three grades of stomatosis of fiber crops (%) | Quality one-level (%) | Quality secondary (%) | Three grades of qualities (%) | Reed head (cm) | Root long (cm) | Lateral root number (cm) |
| CK | 7.42 | 53.98 | 19.46 | 17.69 | 9.73 | 28.84 | 39.22 | 31.94 | 1.70 | 15.18 | 2.27 |
| 2% | 8.75 | 57.81 | 25.00 | 10.93 | 6.25 | 37.03 | 34.17 | 28.80 | 2.25 | 20.67 | 1.80 |
Table 1. 2% preparation group output, Radix Angelicae Sinensis fiber crops stomatosis are prevented and treated result and quality evaluation result
The numb stomatosis incidence of disease of control group is 46.88%, and dispenser sequela rate obviously reduces, and the 2% preparation group incidence of disease is reduced to 42.18%; And be also very significantly improved in dispenser sequela situation, endangering serious secondary, three grades of numb stomatosis incidences of disease significantly reduce, three grades of sick incidences of disease of control group are 9.73%, and three grades of sick incidences of disease of 2% preparation group are only 6.25%.
The output of Radix Angelicae Sinensis and ocular estimate are directly connected to the economic benefit of Radix Angelicae Sinensis.The wherein reed head size of Radix Angelicae Sinensis, root length and lateral root number are the important indicators of Radix Angelicae Sinensis ocular estimate, good quality, the Radix Angelicae Sinensis that price is higher, its reed diameter is large, and main root is longer, and lateral root is less.
Statistical result showed, the gross yield of dispenser group Radix Angelicae Sinensis will be apparently higher than control group, and the gross yield of control group is 7.42 kg, and 2% preparation group is 8.75 kg.A control group reed average diameter is 1.70 cm, and 2% preparation group average diameter is 2.25 cm; Control group average root length is 15.18 cm, and 2% preparation group average root length is 20.67 cm; 2.27 of the average lateral roots of control group, and the average lateral root number of 2% preparation group is 1.80.
In the numb stomatosis of control, said preparation can also effectively improve Radix Angelicae Sinensis emergence rate, reduces the bolting of Radix Angelicae Sinensis, thereby has improved the patent medicine rate of Radix Angelicae Sinensis, and then makes Radix Angelicae Sinensis realize increasing both production and income (in table 2).
| ? | Emerge and count (strain) | Early a kind of sedge number (strain) | Early a kind of sedge rate (%) | Become a useful person and count (strain) | Lumber recovery (%) |
| CK | 317 | 52 | 16.40 | 114 | 35.96 |
| 2% | 364 | 70 | 19.23 | 128 | 35.16 |
The impact that table 2. 2% preparation group is emerged and become a useful person Radix Angelicae Sinensis
Show in field test results, said preparation has certain preventive and therapeutic effect (in table 3) equally to Radix Angelicae Sinensis leaf spot.
| Leaf spot | Without (%) | One-level (%) | Secondary (%) | Three grades (%) |
| CK | 10.06 | 50.65 | 33.33 | 6.67 |
| 2% | 16.67 | 63.33 | 16.67 | 3.33 |
Table 3. 2% impact of preparation group on Radix Angelicae Sinensis leaf spot
The Radix Angelicae Sinensis fingerprint spectrum method that utilizes the present invention to set up, to the content detection result of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis as following table:
| ? | Forulic acid (peak area) | Ligustilide (peak area) |
| CK | 1521.958 | 25232.77 |
| 2% | 1573.826 | 26618.29 |
Table 4. 2% impact of preparation group on effective component in Radix Angelicae Sinensis
Utilize the method for detecting residue of having set up in the present invention to detect the sample of 2% preparation group.In 3 samples that extract, all do not detect that preparation is residual.
Embodiment 2
Prepare 5% preparation, method is as follows:
Root of Stellera chamaejasme through drying, pulverize, sieve, is extracted 4 times to each 26 hours repeatedly by the alcohol immersion of 12 times of quality; Extract, through filtration, reduced pressure concentration, dry, makes stellera chamaejasme L extract;
Water or ethanol water be stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizes cracker to pulverize, and crosses 45 mesh sieves, makes evengranular nematocide.Wherein root of Stellera chamaejasme extract and diatomite proportioning composition in preparation, count root of Stellera chamaejasme extractive content as 5% take quality percentage composition, and diatomite content is 95%.
Select at random 6 continuous ridges in Qin Xu township, Minxian County, Dingxi City of Radix Angelicae Sinensis main growing area Gansu Province and carried out Radix Angelicae Sinensis plantation field trial.Every ridge all long 8m of ground, respectively are control group (CK), 5% preparation dispenser group.Preparation 3 g are evenly sprinkling upon to the root 4 cm places around of Radix Angelicae Sinensis seedling.
Output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation the results are shown in following table:
| ? | Output (Kg) | Fiber crops stomatosis is without (%) | Fiber crops stomatosis one-levels (%) | Fiber crops stomatosis secondarys (%) | Three grades of stomatosis of fiber crops (%) | Quality one-level (%) | Quality secondary (%) | Three grades of qualities (%) | Reed head (cm) | Root long (cm) | Lateral root number (cm) |
| CK | 7.42 | 53.98 | 19.46 | 17.69 | 9.73 | 28.84 | 39.22 | 31.94 | 1.70 | 15.18 | 2.27 |
| 5% | 8.52 | 76.92 | 7.69 | 8.47 | 6.92 | 29.81 | 37.21 | 32.98 | 2.02 | 21.97 | 1.97 |
Table 5. 5% preparation group output, Radix Angelicae Sinensis fiber crops stomatosis are prevented and treated result and quality evaluation result
The numb stomatosis incidence of disease of control group is 46.88%, and dispenser sequela rate obviously reduces, and the 5% preparation group incidence of disease is significantly reduced to 23.08%; And be also very significantly improved in dispenser sequela situation, endangering serious secondary, three grades of numb stomatosis incidences of disease reductions, three grades of sick incidences of disease of control group are 9.73%, and three grades of sick incidences of disease of 5% preparation group are only 6.92%.
The gross yield of dispenser group Radix Angelicae Sinensis will be apparently higher than control group, and the gross yield of control group is 7.42 kg, and 5% preparation group is 8.52 kg.A control group reed average diameter is 1.70 cm, and 5% preparation group average diameter is 2.02 cm; Control group average root length is 15.18 cm, and 5% preparation group average root length is 21.97 cm; 2.27 of the average lateral roots of control group, and the average lateral root number of 5% preparation group is 1.97.
In the numb stomatosis of control, said preparation can also effectively improve Radix Angelicae Sinensis emergence rate, reduces the bolting of Radix Angelicae Sinensis, thereby has improved the patent medicine rate of Radix Angelicae Sinensis, and then makes Radix Angelicae Sinensis realize increasing both production and income (in table 6).
| ? | Emerge and count (strain) | Early a kind of sedge number (strain) | Early a kind of sedge rate (%) | Become a useful person and count (strain) | Lumber recovery (%) |
| CK | 317 | 52 | 16.40 | 114 | 35.96 |
| 5% | 357 | 53 | 14.85 | 130 | 36.41 |
The impact that table 6. 5% preparation group is emerged and become a useful person Radix Angelicae Sinensis
Show in field test results, 5% preparation group has certain preventive and therapeutic effect (in table 7) equally to Radix Angelicae Sinensis leaf spot.
| Leaf spot | Without (%) | One-level (%) | Secondary (%) | Three grades (%) |
| CK | 10.06 | 50.65 | 33.33 | 6.67 |
| 5% | 20.07 | 56.67 | 20.08 | 3.18 |
Table 7. 5% impact of preparation group on Radix Angelicae Sinensis leaf spot
The Radix Angelicae Sinensis fingerprint spectrum method that utilizes the present invention to set up, to the content detection result of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis as following table:
| ? | Forulic acid (peak area) | Ligustilide (peak area) |
| CK | 1521.958 | 25232.77 |
| 5% | 1871.017 | 26847.44 |
Table 8. 5% impact of preparation group on effective component in Radix Angelicae Sinensis
5% preparation group can obviously improve the content of drug effect components of Radix Angelicae Sinensis, improves the medical value of Radix Angelicae Sinensis.Wherein the content of forulic acid exceeds 22.93% than contrast; The content of Ligustilide exceeds 6.40% than contrast.
Utilize the method for detecting residue of having set up in the present invention to detect the sample of 5% preparation group.In 3 samples that extract, all do not detect that preparation is residual.
Embodiment 3
Prepare 10% preparation, method is as follows:
Root of Stellera chamaejasme through drying, pulverize, sieve, is extracted 5 times to each 20 hours repeatedly by the alcohol immersion of 11 times of quality; Extract, through filtration, reduced pressure concentration, dry, makes stellera chamaejasme L extract;
Water or ethanol water be stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizes cracker to pulverize, and crosses 45 mesh sieves, makes evengranular nematocide.Wherein root of Stellera chamaejasme extract and diatomite proportioning composition in preparation, count root of Stellera chamaejasme extractive content as 10% take quality percentage composition, and diatomite content is 90%.
Select at random 6 continuous ridges in Qin Xu township, Minxian County, Dingxi City of Radix Angelicae Sinensis main growing area Gansu Province and carried out Radix Angelicae Sinensis plantation field trial.Every ridge all long 8m of ground, respectively are control group (CK), 10% preparation dispenser group.Preparation 4 g are evenly sprinkling upon to the root 3 cm places around of Radix Angelicae Sinensis seedling.
Output, Radix Angelicae Sinensis fiber crops stomatosis prevents and treats result and quality evaluation the results are shown in following table:
| ? | Output (Kg) | Fiber crops stomatosis is without (%) | Fiber crops stomatosis one-levels (%) | Fiber crops stomatosis secondarys (%) | Three grades of stomatosis of fiber crops (%) | Quality one-level (%) | Quality secondary (%) | Three grades of qualities (%) | Reed head (cm) | Root long (cm) | Lateral root number (cm) |
| CK | 7.42 | 53.98 | 19.46 | 17.69 | 9.73 | 28.84 | 39.22 | 31.94 | 1.70 | 15.18 | 2.27 |
| 10% | 9.42 | 74.03 | 12.99 | 11.04 | 1.94 | 24.42 | 39.17 | 36.41 | 2.39 | 20.80 | 1.73 |
Table 9. 10% preparation group output, Radix Angelicae Sinensis fiber crops stomatosis are prevented and treated result and quality evaluation result
The numb stomatosis incidence of disease of control group is 46.88%, and dispenser sequela rate obviously reduces, and the 10% preparation group incidence of disease is significantly reduced to 25.97%; And be also very significantly improved in dispenser sequela situation, endangering serious secondary, three grades of numb stomatosis incidences of disease significantly reduce, three grades of sick incidences of disease of control group are 9.73%, and three grades of sick incidences of disease of 10% preparation group are only 1.94%.
The gross yield of dispenser group Radix Angelicae Sinensis will be apparently higher than control group, and the gross yield of control group is 7.42 kg, and 10% preparation group is 9.42 kg.A control group reed average diameter is 1.70 cm, and 10% preparation group average diameter is 2.39 cm; Control group average root length is 15.18 cm, and 10% preparation group average root length is 20.80 cm; 2.27 of the average lateral roots of control group, and the average lateral root number of 10% preparation group is 1.73.
In the numb stomatosis of control, said preparation can also effectively improve Radix Angelicae Sinensis emergence rate, reduces the bolting of Radix Angelicae Sinensis, thereby has improved the patent medicine rate of Radix Angelicae Sinensis, and then makes Radix Angelicae Sinensis realize increasing both production and income (in table 10).
| ? | Emerge and count (strain) | Early a kind of sedge number (strain) | Early a kind of sedge rate (%) | Become a useful person and count (strain) | Lumber recovery (%) |
| CK | 317 | 52 | 16.40 | 114 | 35.96 |
| 10% | 367 | 72 | 19.62 | 154 | 41.96 |
The impact that table 10. 10% preparation group is emerged and become a useful person Radix Angelicae Sinensis
Show in field test results, said preparation has certain preventive and therapeutic effect (in table 7) equally to Radix Angelicae Sinensis leaf spot.
| Leaf spot | Without (%) | One-level (%) | Secondary (%) | Three grades (%) |
| CK | 10.06 | 50.65 | 33.33 | 6.67 |
| 10% | 26.67 | 53.33 | 20.00 | 0 |
Table 11. 10% impact of preparation group on Radix Angelicae Sinensis leaf spot
Utilize the Radix Angelicae Sinensis fingerprint spectrum method of having set up in the present invention, to the content detection result of drug effect Ferulic Acid and Ligustilide in Radix Angelicae Sinensis as following table:
| ? | Forulic acid (peak area) | Ligustilide (peak area) |
| CK | 1521.958 | 25232.77 |
| 10% | 2240.5 | 29208.45 |
Table 12. 10% impact of preparation group on effective component in Radix Angelicae Sinensis
10% preparation group obviously improves the content of drug effect components of Radix Angelicae Sinensis, improves the medical value of Radix Angelicae Sinensis.Wherein the content of forulic acid exceeds 47.21% than contrast; The content of Ligustilide exceeds 15.76% than contrast.
Utilize the method for detecting residue of having set up in the present invention to detect the sample of 10% preparation group.In 3 samples that extract, all do not detect that preparation is residual.
Claims (5)
1. prevent and treat the plant resource nematocide of Radix Angelicae Sinensis fiber crops stomatosis for one kind, it is characterized in that nematocide is made up of stellera chamaejasme L extract and diatomite proportioning, it is 2-15% that each component and content are counted stellera chamaejasme L extract content with quality percentage composition, and diatomite content is 85-98%; Prepare by following method successively:
A, by Chinese Stellera Root through drying, pulverize, sieve, repeatedly extract by alcohol immersion; Extract, through filtration, reduced pressure concentration, dry, makes stellera chamaejasme L extract;
B, water or ethanol water be stellera chamaejasme L extract hydrotropy, then diatomite is added wherein, by stirring, drying under reduced pressure, utilizes cracker to pulverize, and crosses 45 mesh sieves, makes evengranular nematocide.
2. in A step, the mass ratio of Chinese Stellera Root and ethanol is 1:6-20.
3. the plant resource nematocide of control Radix Angelicae Sinensis fiber crops stomatosis as claimed in claim 1, is characterized in that in A step, and the mass ratio of Chinese Stellera Root and ethanol is 1:6-20.
4. the plant resource nematocide of control Radix Angelicae Sinensis fiber crops stomatosis as claimed in claim 1, is characterized in that in A step, and the number of times repeatedly extracting is 2 to 5 times, and each extraction time is 20 to 30 hours.
5. a nematocide as claimed in claim 1 is as control Radix Angelicae Sinensis fiber crops stomatosis, it is characterized in that Radix Angelicae Sinensis fiber crops stomatosis is had to inhibitory action, in Radix Angelicae Sinensis transplanting time and vegetative period process, be applied to Radix Angelicae Sinensis plantation around in 1-8 cm place soil, every strain chemical applied to plants amount is at 2-6 g.
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| CN106105613A (en) * | 2016-06-24 | 2016-11-16 | 渭源县鹿鸣现代农业专业合作社 | A kind of prevention and controls of Radix Angelicae Sinensis fiber crops stomatosis |
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| CN108033858A (en) * | 2017-12-19 | 2018-05-15 | 河南省农业科学院园艺研究所 | A kind of dedicated clayey soil modifying agent of industrialized agriculture |
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| CN105669315A (en) * | 2016-02-22 | 2016-06-15 | 甘肃省农业科学院植物保护研究所 | Plant source radix euphorbiae lantu insecticide and fertilizer mixture granule and preparation method |
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| CN112903876A (en) * | 2021-03-02 | 2021-06-04 | 中国科学院兰州化学物理研究所 | Method for extracting root soil and root allelochemical substances of stellera chamaejasme and detecting content of root allelochemical substances |
| CN112903876B (en) * | 2021-03-02 | 2022-04-01 | 中国科学院兰州化学物理研究所 | Method for extracting root soil and root allelochemical substances of stellera chamaejasme and detecting content of root allelochemical substances |
| CN116210709A (en) * | 2023-03-14 | 2023-06-06 | 郑州大学 | Application of 5-O-methyl-vidamiloride in plant root system development |
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